LOCUS       NM_170753               2266 bp    mRNA    linear   PRI 03-JUN-2024
DEFINITION  Homo sapiens piggyBac transposable element derived 3 (PGBD3), mRNA.
ACCESSION   NM_170753
VERSION     NM_170753.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Weiner,A.M. and Gray,L.T.
  TITLE     What role (if any) does the highly conserved CSB-PGBD3 fusion
            protein play in Cockayne syndrome?
  JOURNAL   Mech Ageing Dev 134 (5-6), 225-233 (2013)
   PUBMED   23369858
  REMARK    Review article
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Gray,L.T., Fong,K.K., Pavelitz,T. and Weiner,A.M.
  TITLE     Tethering of the conserved piggyBac transposase fusion protein
            CSB-PGBD3 to chromosomal AP-1 proteins regulates expression of
            nearby genes in humans
  JOURNAL   PLoS Genet 8 (9), e1002972 (2012)
   PUBMED   23028371
REFERENCE   3  (bases 1 to 2266)
  AUTHORS   Bailey,A.D., Gray,L.T., Pavelitz,T., Newman,J.C., Horibata,K.,
            Tanaka,K. and Weiner,A.M.
  TITLE     The conserved Cockayne syndrome B-piggyBac fusion protein
            (CSB-PGBD3) affects DNA repair and induces both interferon-like and
            innate antiviral responses in CSB-null cells
  JOURNAL   DNA Repair (Amst) 11 (5), 488-501 (2012)
   PUBMED   22483866
  REMARK    GeneRIF: CSB-PGBD3 fusion protein is important in both health and
            disease, and could play a role in Cockayne syndrome.
REFERENCE   4  (bases 1 to 2266)
  AUTHORS   Horibata,K., Saijo,M., Bay,M.N., Lan,L., Kuraoka,I., Brooks,P.J.,
            Honma,M., Nohmi,T., Yasui,A. and Tanaka,K.
  TITLE     Mutant Cockayne syndrome group B protein inhibits repair of DNA
            topoisomerase I-DNA covalent complex
  JOURNAL   Genes Cells 16 (1), 101-114 (2011)
   PUBMED   21143350
REFERENCE   5  (bases 1 to 2266)
  AUTHORS   Newman,J.C., Bailey,A.D., Fan,H.Y., Pavelitz,T. and Weiner,A.M.
  TITLE     An abundant evolutionarily conserved CSB-PiggyBac fusion protein
            expressed in Cockayne syndrome
  JOURNAL   PLoS Genet 4 (3), e1000031 (2008)
   PUBMED   18369450
  REMARK    GeneRIF: a domesticated PiggyBac-like transposon PGBD3, residing
            within intron 5 of the CSB gene, functions as an alternative 3'
            terminal exon
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2266)
  AUTHORS   Sarkar,A., Sim,C., Hong,Y.S., Hogan,J.R., Fraser,M.J.,
            Robertson,H.M. and Collins,F.H.
  TITLE     Molecular evolutionary analysis of the widespread piggyBac
            transposon family and related 'domesticated' sequences
  JOURNAL   Mol Genet Genomics 270 (2), 173-180 (2003)
   PUBMED   12955498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Hugh Robertson. The reference sequence was
            derived from BC063690.1 and AL138760.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 4, 2013 this sequence version replaced NM_170753.2.
            
            Summary: This gene is a member of a small family of genes derived
            from piggyBac transposable elements. The encoded protein contains a
            zinc-ribbon domain characteristic of transposon-derived proteins
            and may function as a regulator of transcription. Alternative
            splicing occurs between a splice site from exon 5 of the adjacent
            upstream gene 'excision repair cross-complementation group 6'
            (ERCC6, GeneID: 2074) and the 3' splice site upstream of the open
            reading frame (ORF) of this gene, which activates the alternative
            polyadenylation site downstream of the piggyback-derived-3 ORF. The
            resulting transcripts encode a fusion protein that shares sequence
            with the product of each individual gene. Pseudogenes for this gene
            are defined on chromosomes 4, 5 and 12. [provided by RefSeq, Mar
            2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063690.1, SRR1660809.197215.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1400              BC063690.1         1-1400
            1401-2266           AL138760.14        153484-154349       c
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..2266
                     /gene="PGBD3"
                     /note="piggyBac transposable element derived 3"
                     /db_xref="GeneID:267004"
                     /db_xref="HGNC:HGNC:19400"
     exon            1..249
                     /gene="PGBD3"
                     /inference="alignment:Splign:2.1.0"
     exon            250..2266
                     /gene="PGBD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..2038
                     /gene="PGBD3"
                     /codon_start=1
                     /product="piggyBac transposable element-derived protein 3"
                     /protein_id="NP_736609.2"
                     /db_xref="GeneID:267004"
                     /db_xref="HGNC:HGNC:19400"
                     /translation="MPRTLSLHEITDLLETDDSIEASAIVIQPPENATAPVSDEESGD
                     EEGGTINNLPGSLLHTAAYLIQDGSDAESDSDDPSYAPKDDSPDEVPSTFTVQQPPPS
                     RRRKMTKILCKWKKADLTVQPVAGRVTAPPNDFFTVMRTPTEILELFLDDEVIELIVK
                     YSNLYACSKGVHLGLTSSEFKCFLGIIFLSGYVSVPRRRMFWEQRTDVHNVLVSAAMR
                     RDRFETIFSNLHVADNANLDPVDKFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPY
                     FGRHGCKQFIRGKPIRFGYKFWCGATCLGYICWFQPYQGKNPNTKHEEYGVGASLVLQ
                     FSEALTEAHPGQYHFVFNNFFTSIALLDKLSSMGHQATGTVRKDHIDRVPLESDVALK
                     KKERGTFDYRIDGKGNIVCRWNDNSVVTVASSGAGIHPLCLVSRYSQKLKKKIQVQQP
                     NMIKVYNQFMGGVDRADENIDKYRASIRGKKWYSSPLLFCFELVLQNAWQLHKTYDEK
                     PVDFLEFRRRVVCHYLETHGHPPEPGQKGRPQKRNIDSRYDGINHVIVKQGKQTRCAE
                     CHKNTTFRCEKCDVALHVKCSVEYHTE"
     regulatory      2150..2155
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGBD3"
                     /note="hexamer: AATAAA"
     polyA_site      2173
                     /gene="PGBD3"
                     /note="major polyA site"
ORIGIN      
        1 ggaagatgac gaggtggagg gggcagaggc ggacctgtct ggagatggta ctgactatga
       61 gctgaagcct ctgcccaagg gcgggaaacg gcagaagaaa gtgccagtgc aggagattga
      121 tgatgacttt ttcccaagtt ctggggaaga agctgaagct gcttctgtag gagaaggagg
      181 aggaggaggt cggaaagtgg gaagataccg agatgatgga gatgaagatt attataagca
      241 gcggttaagt cccaagatgc ctcgaacact aagtttacat gaaataactg accttttaga
      301 gacagatgac agcatagaag caagtgctat agtgatacaa ccacctgaaa atgctacagc
      361 acctgtttct gatgaggaat caggagatga agaaggtgga acaataaata atctgccagg
      421 ttctttgttg cacacagctg cgtatcttat tcaagatggc tctgatgctg agtctgactc
      481 agatgatccc tcatacgcac ctaaagatga ctctcctgat gaagttccat ctacgtttac
      541 tgtgcagcaa cctccaccat caaggaggag gaaaatgaca aaaattcttt gcaaatggaa
      601 aaaagccgac ctaactgtac aacccgtagc aggtagagtt acagcaccac caaacgattt
      661 cttcaccgta atgagaactc ccacagaaat tcttgaactt tttcttgatg acgaggtcat
      721 tgaactcatt gtcaagtact ccaacttata tgcttgcagt aaaggtgtac atcttggctt
      781 gactagctct gaattcaaat gttttctggg aattattttt ctgagtggtt atgtctcagt
      841 tcctagaagg cgtatgtttt gggaacaaag aacagatgtg cataatgtac tggttagtgc
      901 tgccatgaga cgtgaccggt ttgaaactat attttctaat ttgcatgttg ctgacaatgc
      961 aaatttggat ccagtggaca aattttccaa attgcgacct ctcataagca aacttaatga
     1021 gagatgcatg aaatttgttc caaatgaaac atatttcagc tttgatgaat tcatggttcc
     1081 ttattttggt cgtcacgggt gcaaacaatt tattcgggga aagcccattc ggtttggcta
     1141 taagttttgg tgtggtgcca cctgtctggg ctacatttgc tggtttcagc cgtatcaggg
     1201 taaaaaccca aatactaaac atgaggaata tggtgtcggt gcgtcacttg tccttcagtt
     1261 tagtgaggca cttacagagg cacaccctgg acaataccat tttgtattca ataacttttt
     1321 caccagtatt gcacttcttg ataagctcag ttcaatggga catcaggcaa caggtacagt
     1381 gagaaaggat cacattgaca gagttccact ggaatcagat gtagctttaa agaaaaaaga
     1441 aagaggcaca tttgattatc gaattgatgg caaaggcaat attgtctgca gatggaatga
     1501 taacagtgtt gtcactgttg cctcatctgg tgctggtatc catcccctgt gtcttgtcag
     1561 tcgttactcc cagaaactga aaaagaagat acaagttcag cagccaaaca tgatcaaagt
     1621 gtataaccag ttcatgggag gcgtagacag agctgatgaa aacattgata agtatcgggc
     1681 atcaatccgt ggaaagaaat ggtattcaag ccctcttttg ttctgtttcg aactggtctt
     1741 acaaaatgct tggcaattgc ataaaacata tgatgagaaa ccagtggatt ttctggagtt
     1801 tcgtcgacgt gtggtatgcc attatctgga gacccatggt catcctccag aacctggcca
     1861 aaaaggaaga cctcagaagc gtaacattga ctcacgttat gatggcataa atcatgtgat
     1921 agtcaaacag ggaaagcaaa cgcgatgcgc tgaatgtcat aagaacacaa cttttcgatg
     1981 tgaaaaatgt gatgttgcct tacatgtgaa gtgttccgtt gaatatcaca ctgaatagca
     2041 ggtgtcacca cctcctgaga taagaaacat agttttatac attatgtaca gtgtagcagt
     2101 ggttttgcct agtgttccaa ttttggaacg tcacataaca atggaacata ataaattttt
     2161 ttttctcttc aaatttttgt tccttgaatt tttctaggta acatatatga atttcatgca
     2221 aaaattcaaa aaattgtaac ctcagacata aatgggttaa agatgt
//
