LOCUS       NM_021259               3672 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens post-GPI attachment to proteins 6 (PGAP6), mRNA.
ACCESSION   NM_021259
VERSION     NM_021259.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Lee,G.H., Fujita,M., Nakanishi,H., Miyata,H., Ikawa,M., Maeda,Y.,
            Murakami,Y. and Kinoshita,T.
  TITLE     PGAP6, a GPI-specific phospholipase A2, has narrow substrate
            specificity against GPI-anchored proteins
  JOURNAL   J Biol Chem 295 (42), 14501-14509 (2020)
   PUBMED   32816994
  REMARK    GeneRIF: PGAP6, a GPI-specific phospholipase A2, has narrow
            substrate specificity against GPI-anchored proteins.
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3672)
  AUTHORS   Lee,G.H., Fujita,M., Takaoka,K., Murakami,Y., Fujihara,Y.,
            Kanzawa,N., Murakami,K.I., Kajikawa,E., Takada,Y., Saito,K.,
            Ikawa,M., Hamada,H., Maeda,Y. and Kinoshita,T.
  TITLE     A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling
            in embryos by shedding CRIPTO
  JOURNAL   J Cell Biol 215 (5), 705-718 (2016)
   PUBMED   27881714
  REMARK    GeneRIF: PGAP6 plays a critical role in Nodal signaling modulation
            through CRIPTO shedding.
REFERENCE   4  (bases 1 to 3672)
  AUTHORS   Palmieri,M., Impey,S., Kang,H., di Ronza,A., Pelz,C., Sardiello,M.
            and Ballabio,A.
  TITLE     Characterization of the CLEAR network reveals an integrated control
            of cellular clearance pathways
  JOURNAL   Hum Mol Genet 20 (19), 3852-3866 (2011)
   PUBMED   21752829
  REMARK    GeneRIF: TMEM8A protein localizes in lysosomes in HeLa cells
REFERENCE   5  (bases 1 to 3672)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   6  (bases 1 to 3672)
  AUTHORS   Martin,J., Han,C., Gordon,L.A., Terry,A., Prabhakar,S., She,X.,
            Xie,G., Hellsten,U., Chan,Y.M., Altherr,M., Couronne,O., Aerts,A.,
            Bajorek,E., Black,S., Blumer,H., Branscomb,E., Brown,N.C.,
            Bruno,W.J., Buckingham,J.M., Callen,D.F., Campbell,C.S.,
            Campbell,M.L., Campbell,E.W., Caoile,C., Challacombe,J.F.,
            Chasteen,L.A., Chertkov,O., Chi,H.C., Christensen,M., Clark,L.M.,
            Cohn,J.D., Denys,M., Detter,J.C., Dickson,M.,
            Dimitrijevic-Bussod,M., Escobar,J., Fawcett,J.J., Flowers,D.,
            Fotopulos,D., Glavina,T., Gomez,M., Gonzales,E., Goodstein,D.,
            Goodwin,L.A., Grady,D.L., Grigoriev,I., Groza,M., Hammon,N.,
            Hawkins,T., Haydu,L., Hildebrand,C.E., Huang,W., Israni,S.,
            Jett,J., Jewett,P.B., Kadner,K., Kimball,H., Kobayashi,A.,
            Krawczyk,M.C., Leyba,T., Longmire,J.L., Lopez,F., Lou,Y., Lowry,S.,
            Ludeman,T., Manohar,C.F., Mark,G.A., McMurray,K.L., Meincke,L.J.,
            Morgan,J., Moyzis,R.K., Mundt,M.O., Munk,A.C., Nandkeshwar,R.D.,
            Pitluck,S., Pollard,M., Predki,P., Parson-Quintana,B., Ramirez,L.,
            Rash,S., Retterer,J., Ricke,D.O., Robinson,D.L., Rodriguez,A.,
            Salamov,A., Saunders,E.H., Scott,D., Shough,T., Stallings,R.L.,
            Stalvey,M., Sutherland,R.D., Tapia,R., Tesmer,J.G., Thayer,N.,
            Thompson,L.S., Tice,H., Torney,D.C., Tran-Gyamfi,M., Tsai,M.,
            Ulanovsky,L.E., Ustaszewska,A., Vo,N., White,P.S., Williams,A.L.,
            Wills,P.L., Wu,J.R., Wu,K., Yang,J., Dejong,P., Bruce,D.,
            Doggett,N.A., Deaven,L., Schmutz,J., Grimwood,J., Richardson,P.,
            Rokhsar,D.S., Eichler,E.E., Gilna,P., Lucas,S.M., Myers,R.M.,
            Rubin,E.M. and Pennacchio,L.A.
  TITLE     The sequence and analysis of duplication-rich human chromosome 16
  JOURNAL   Nature 432 (7020), 988-994 (2004)
   PUBMED   15616553
REFERENCE   7  (bases 1 to 3672)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
REFERENCE   8  (bases 1 to 3672)
  AUTHORS   Motohashi,T., Miyoshi,S., Osawa,M., Eyre,H.J., Sutherland,G.R.,
            Matsuda,Y., Nakamura,Y., Shibuya,A., Iwama,A. and Nakauchi,H.
  TITLE     Molecular cloning and chromosomal mapping of a novel five-span
            transmembrane protein gene, M83
  JOURNAL   Biochem Biophys Res Commun 276 (1), 244-250 (2000)
   PUBMED   11006113
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            Z97634.26, AL537021.3, AB045292.1, BC021557.1, DB224383.1,
            AL546643.3, BC004276.1 and AI678371.1.
            
            On Nov 23, 2018 this sequence version replaced NM_021259.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB045292.1, BC021557.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000431232.7/ ENSP00000401338.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                Z97634.26          16068-16095         c
            29-124              AL537021.3         6-101
            125-137             AB045292.1         54-66
            138-306             BC021557.1         1-169
            307-628             DB224383.1         134-455
            629-1084            BC021557.1         492-947
            1085-1085           AL546643.3         112-112
            1086-2595           BC021557.1         949-2458
            2596-3552           BC004276.1         593-1549
            3553-3672           AI678371.1         13-132              c
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..3672
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /note="post-GPI attachment to proteins 6"
                     /db_xref="GeneID:58986"
                     /db_xref="HGNC:HGNC:17205"
                     /db_xref="MIM:619342"
     exon            1..278
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..2473
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /EC_number="3.1.1.4"
                     /note="transmembrane protein 6; transmembrane protein 8
                     (five membrane-spanning domains); five-span transmembrane
                     protein M83; GPI processing phospholipase A2; post-GPI
                     attachment to proteins factor 6; transmembrane protein 8A;
                     post-glycosylphosphatidylinositol attachment to proteins
                     6"
                     /codon_start=1
                     /product="post-GPI attachment to proteins factor 6
                     precursor"
                     /protein_id="NP_067082.2"
                     /db_xref="CCDS:CCDS10407.1"
                     /db_xref="GeneID:58986"
                     /db_xref="HGNC:HGNC:17205"
                     /db_xref="MIM:619342"
                     /translation="MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGL
                     VSEHFSQAPQRLSFYSWYGSARLFRFRVPPDAVLLRWLLQVSRESGAACTDAEITVHF
                     RSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSNASVNVSHPAPGDWFVAAHLP
                     PSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSYLKVFVPDY
                     TRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDR
                     WLQVTAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPD
                     HQDLGRSGRVDRSPFCLTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNT
                     GMDSGGSLTISLRANKTEMRNETVVVACVNAASPFLGFNTSLNCTTAFFQGYPLSLSA
                     WSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVETTLYLVPCLNDCGPYGQC
                     LLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLAPIAVSV
                     RRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILC
                     MARLKTVLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQ
                     CYPTSWQRWAFYLLPGVSMASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPP
                     DQPAEPWACSQKFPCHYQICKNDREELYAVT"
     exon            279..456
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            457..664
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            665..792
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            793..1061
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1381
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1472
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1596
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1733
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1912
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2059
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2176
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..3672
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2576..2581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /note="hexamer: AATAAA"
     polyA_site      2598
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /note="major polyA site"
     regulatory      3573..3578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /note="hexamer: AGTAAA"
     polyA_site      3621
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
     polyA_site      3672
                     /gene="PGAP6"
                     /gene_synonym="GPI-PLA2; M83; TMEM6; TMEM8; TMEM8A"
                     /note="major polyA site"
ORIGIN      
        1 agtgcccggg gcgacggcgg cggggaccgg ccgggccatg ggcgccgggg gcagaggcgg
       61 aggcggcaga ggcggaggcg gcagaggctg cccgggcggc ggcgggggcc ggcggcgcgg
      121 gacgcgggcc gggtagcgcc gggccgagcg cggagccatg ggccgggctg gcaccgggac
      181 cgggggcgag gcggtggccg cggtggtggc ggggccgctg ctgctgctgc tgcttgcccg
      241 gcccccgcct gcctccgccg gctacagcgg gaagagcgag gtggggctgg tgtccgagca
      301 cttctcgcag gccccgcaga ggctgtcctt ctacagctgg tacggcagtg ccaggctctt
      361 ccgcttccgc gtgcccccag atgctgtgct tctacgctgg ctcctgcagg tctcccggga
      421 gagcggcgct gcctgcaccg acgcggagat caccgtgcac ttccgttccg gcgcccctcc
      481 ggtcatcaac ccgctgggca ccagcttccc ggacgacacc gcggtacagc cctccttcca
      541 ggtcggggtg ccgctgagca ccacaccgag aagcaatgcc tccgtcaacg tttcccaccc
      601 ggcccccggg gactggttcg tggccgccca cctgcccccc tcatcccaga agatcgagtt
      661 gaagggcttg gctcccacct gtgcctacgt cttccagcct gaactgctgg tcacgcgggt
      721 ggtcgagatt tccatcatgg agccggacgt gccccttcct cagaccctcc tctcccatcc
      781 cagctacctc aaggtctttg tccccgatta cacgcgggag cttctgctgg agctgcggga
      841 ctgcgtgtcc aatgggagcc tgggctgccc cgtgcgtctc accgtgggcc cggtcaccct
      901 gcctagcaac ttccagaagg tgctcacctg caccggtgcc ccctggccct gccgcctgct
      961 gctgccctca ccgccctggg accggtggct gcaagtgaca gctgagagcc tggtggggcc
     1021 cctcgggaca gtggctttca gtgctgtagc tgccctcaca gcttgcaggc cacggagcgt
     1081 gaccatccag ccccttctgc agagcagcca aaaccagagc ttcaatgcct cctctggtct
     1141 gctgtccccg agccccgacc accaggacct gggcaggagt ggcagggtgg accgcagccc
     1201 cttctgcctc acaaactacc cagtcacgcg ggaggacatg gacgtggtgt cggtgcactt
     1261 ccagcccctg gacagggtct cggtgagggt gtgttcggac acgccctccg tgatgcggct
     1321 gcgcctgaac accggcatgg acagcggggg ttccctcacc atctccctgc gggccaacaa
     1381 gacagagatg cggaacgaga ccgtcgtagt ggcctgcgtg aatgctgcct cgcccttcct
     1441 tggcttcaat acttcgctca actgcaccac agccttcttc cagggctacc ctttgtctct
     1501 gagcgcctgg tctcgcaggg ccaacctcat catcccctac ccagagacag acaactggta
     1561 cctctccctg cagctcatgt gccctgagaa tgctgaggac tgtgagcagg ctgtggtcca
     1621 cgtggagacc accttgtacc tggtgccctg tttgaacgat tgtggaccct atggccagtg
     1681 cctcctgctc cgcagacaca gctacctgta tgccagctgc agctgcaagg caggctggcg
     1741 tgggtggagc tgcacggaca acagcacagc ccagacggtg gcccagcaga gggcggccac
     1801 actgctgctc acgctcagca acctcatgtt cctggccccc atcgccgtct cagtgcggcg
     1861 attcttcctg gtggaggcct ccgtctacgc ctacaccatg ttcttctcca cgttctacca
     1921 cgcctgcgac cagcccgggg aggcggtgct gtgcatcctc agctacgaca cgctgcagta
     1981 ctgcgacttc ttgggctccg gggcggccat ctgggtcacc atcctgtgca tggcacggct
     2041 caagacagtc ctgaaatacg tgctgtttct tctgggtaca ctggtcatcg ccatgtcctt
     2101 gcagctggac cgcaggggca tgtggaacat gctggggccc tgcctctttg ccttcgtgat
     2161 catggcctcc atgtgggctt accgctgcgg gcaccggcgc cagtgctacc ccacctcgtg
     2221 gcagcgctgg gccttctacc tcctgcccgg cgtctctatg gcctctgtgg gcatcgccat
     2281 ctacacctcc atgatgacta gcgacaacta ctactacacc cacagcatct ggcacatcct
     2341 gctggccggg agcgcagcct tgctgctgcc gccacctgac cagcccgccg agccctgggc
     2401 ctgctcgcag aaattcccct gccactatca gatctgcaag aacgatcggg aggaactgta
     2461 cgcagtgacg tgacactggc ctggggacag ctgctgctct gatgacctct tcagccagga
     2521 gctgtatcga gggggaggcg cctggtccag ccctggacag attgatttcc agctgaataa
     2581 attggcctag ataccctctt acttcctcct actgaggaga gggccacccc tccctcacac
     2641 gcacctctcc tcgccagctg cctgctcgct gctgagatcc ctctgcagat cccagctgct
     2701 gtctgcagtg gcccctgggg cctgggcttg gttacttgga gaccacaggt gcctgtgcga
     2761 gggtggggct gtgtgctctg ctggcctgtg ggtggctaga gatggggaga aggactccct
     2821 atggtcctgt tccctggcga ggctgtggtg agacccatgg cgcccaggag ccctgagccc
     2881 accactgggg ccgggagctg tggactccga gacccctgct cctggcccct gtgtggccct
     2941 gatgccagcc tgcctggggc atggaggatc cccctctgct gctgtggtca ccatagggac
     3001 gggagacgga ggtttccccc agagctgatg ggcactgggg atgcaggctc ccgccccgcc
     3061 cctccctttt tcctggctct ggagccttgg gtccctgaga agagcttcct tggactgggg
     3121 tgctctgctg ctctgggggt ttggcccaga aggaacccct acccagccct gtccacccac
     3181 cccagcgttc ctgggccccc ctctgggagc ttcctcttgg tcccaggagc tgcggtggtg
     3241 tctgccccct gcactgggct ctgtgccctg tgggtgttca ggagactgga ctgtctctgc
     3301 tcctcatgca aaccttgtgg gggaagcaat gaccaggagc agcacctggc ccctccctga
     3361 ggcctgtgag tgtctcggat ccgccctgga gaagtgagtg gctgagaccc gggtggggcc
     3421 ctcctaccct tggctctggg ggcttccctt ctgggtcttg tccgggatat ggccttcctg
     3481 aaggtcttac ctcccccagc cgccctgcac ttgaccacca aagcaatctc taagcttata
     3541 aaggagaaac atatatatat ggggtttttt ggagtaaagg aaaattctaa gatattaaga
     3601 catattttta tgtagatctg atctatctgt gtttggtgaa tttaatattc tacattgatt
     3661 tttcaaacta aa
//
