LOCUS       NM_024989              11090 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens post-GPI attachment to proteins inositol deacylase 1
            (PGAP1), transcript variant 1, mRNA.
ACCESSION   NM_024989
VERSION     NM_024989.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Hong,J., Li,T., Chao,Y., Xu,Y., Zhu,Z., Zhou,Z., Gu,W., Qu,Q. and
            Li,D.
  TITLE     Molecular basis of the inositol deacylase PGAP1 involved in quality
            control of GPI-AP biogenesis
  JOURNAL   Nat Commun 15 (1), 8 (2024)
   PUBMED   38167496
  REMARK    GeneRIF: Molecular basis of the inositol deacylase PGAP1 involved
            in quality control of GPI-AP biogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Huang,F., Su,Z., Yang,J., Zhao,X. and Xu,Y.
  TITLE     Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1
            axis to aggravate the development of osteoarthritis
  JOURNAL   J Orthop Surg Res 18 (1), 670 (2023)
   PUBMED   37691099
  REMARK    GeneRIF: Downregulation of lncRNA NEAT1 interacts with
            miR-374b-5p/PGAP1 axis to aggravate the development of
            osteoarthritis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11090)
  AUTHORS   Li,H., Lian,K., Mao,J., Huang,F., Zhang,C. and Zang,J.
  TITLE     LncRNA LEMD1-AS1 relieves chondrocyte inflammation by targeting
            miR-944/PGAP1 in osteoarthritis
  JOURNAL   Cell Cycle 21 (19), 2038-2050 (2022)
   PUBMED   35686740
  REMARK    GeneRIF: LncRNA LEMD1-AS1 relieves chondrocyte inflammation by
            targeting miR-944/PGAP1 in osteoarthritis.
REFERENCE   4  (bases 1 to 11090)
  AUTHORS   Bosch,D.G., Boonstra,F.N., Kinoshita,T., Jhangiani,S., de Ligt,J.,
            Cremers,F.P., Lupski,J.R., Murakami,Y. and de Vries,B.B.
  TITLE     Cerebral visual impairment and intellectual disability caused by
            PGAP1 variants
  JOURNAL   Eur J Hum Genet 23 (12), 1689-1693 (2015)
   PUBMED   25804403
  REMARK    GeneRIF: PGAP1 mutation and a proven functional loss of PGAP1 were
            found in a patient with cerebral visual impairment and intellectual
            diasability.
REFERENCE   5  (bases 1 to 11090)
  AUTHORS   Granzow,M., Paramasivam,N., Hinderhofer,K., Fischer,C.,
            Chotewutmontri,S., Kaufmann,L., Evers,C., Kotzaeridou,U.,
            Rohrschneider,K., Schlesner,M., Sturm,M., Pinkert,S., Eils,R.,
            Bartram,C.R., Bauer,P. and Moog,U.
  TITLE     Loss of function of PGAP1 as a cause of severe encephalopathy
            identified by Whole Exome Sequencing: Lessons of the bioinformatics
            pipeline
  JOURNAL   Mol Cell Probes 29 (5), 323-329 (2015)
   PUBMED   26050939
  REMARK    GeneRIF: Study confirms homozygous loss-of function mutations in
            PGAP1 as a cause of severe encephalopathy.
REFERENCE   6  (bases 1 to 11090)
  AUTHORS   Novarino,G., Fenstermaker,A.G., Zaki,M.S., Hofree,M., Silhavy,J.L.,
            Heiberg,A.D., Abdellateef,M., Rosti,B., Scott,E., Mansour,L.,
            Masri,A., Kayserili,H., Al-Aama,J.Y., Abdel-Salam,G.M.H.,
            Karminejad,A., Kara,M., Kara,B., Bozorgmehri,B., Ben-Omran,T.,
            Mojahedi,F., El Din Mahmoud,I.G., Bouslam,N., Bouhouche,A.,
            Benomar,A., Hanein,S., Raymond,L., Forlani,S., Mascaro,M.,
            Selim,L., Shehata,N., Al-Allawi,N., Bindu,P.S., Azam,M., Gunel,M.,
            Caglayan,A., Bilguvar,K., Tolun,A., Issa,M.Y., Schroth,J.,
            Spencer,E.G., Rosti,R.O., Akizu,N., Vaux,K.K., Johansen,A.,
            Koh,A.A., Megahed,H., Durr,A., Brice,A., Stevanin,G., Gabriel,S.B.,
            Ideker,T. and Gleeson,J.G.
  TITLE     Exome sequencing links corticospinal motor neuron disease to common
            neurodegenerative disorders
  JOURNAL   Science 343 (6170), 506-511 (2014)
   PUBMED   24482476
REFERENCE   7  (bases 1 to 11090)
  AUTHORS   Ueda,Y., Yamaguchi,R., Ikawa,M., Okabe,M., Morii,E., Maeda,Y. and
            Kinoshita,T.
  TITLE     PGAP1 knock-out mice show otocephaly and male infertility
  JOURNAL   J Biol Chem 282 (42), 30373-30380 (2007)
   PUBMED   17711852
REFERENCE   8  (bases 1 to 11090)
  AUTHORS   Tanaka,S., Maeda,Y., Tashima,Y. and Kinoshita,T.
  TITLE     Inositol deacylation of glycosylphosphatidylinositol-anchored
            proteins is mediated by mammalian PGAP1 and yeast Bst1p
  JOURNAL   J Biol Chem 279 (14), 14256-14263 (2004)
   PUBMED   14734546
  REMARK    GeneRIF: PGAP1 encoded an ER-associated, 922-amino acid membrane
            protein bearing a lipase consensus motif.
REFERENCE   9  (bases 1 to 11090)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   10 (bases 1 to 11090)
  AUTHORS   Ploug,M., Ronne,E., Behrendt,N., Jensen,A.L., Blasi,F. and Dano,K.
  TITLE     Cellular receptor for urokinase plasminogen activator.
            Carboxyl-terminal processing and membrane anchoring by
            glycosyl-phosphatidylinositol
  JOURNAL   J Biol Chem 266 (3), 1926-1933 (1991)
   PUBMED   1846368
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK022439.1, AB128038.1 and
            AC012486.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_024989.3.
            
            Summary: The protein encoded by this gene functions early in the
            glycosylphosphatidylinositol (GPI) biosynthetic pathway, catalyzing
            the inositol deacylation of GPI. The encoded protein is required
            for the production of GPI that can attach to proteins, and this may
            be an important factor in the transport of GPI-anchored proteins
            from the endoplasmic reticulum to the Golgi. Defects in this gene
            are a cause an autosomal recessive form of cognitive impairment.
            [provided by RefSeq, Jul 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.86063.1,
                                           SRR1803612.240698.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354764.9/ ENSP00000346809.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1001              AK022439.1         24-1024
            1002-2860           AB128038.1         911-2769
            2861-11090          AC012486.9         91529-99758         c
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.1"
     gene            1..11090
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="post-GPI attachment to proteins inositol deacylase
                     1"
                     /db_xref="GeneID:80055"
                     /db_xref="HGNC:HGNC:25712"
                     /db_xref="MIM:611655"
     exon            1..238
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..2860
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="isoform 1 is encoded by transcript variant 1; GPI
                     inositol-deacylase; post-GPI attachment to proteins factor
                     1; post-GPI attachment to proteins 1"
                     /codon_start=1
                     /product="GPI inositol-deacylase isoform 1"
                     /protein_id="NP_079265.2"
                     /db_xref="CCDS:CCDS2318.1"
                     /db_xref="GeneID:80055"
                     /db_xref="HGNC:HGNC:25712"
                     /db_xref="MIM:611655"
                     /translation="MFLHSVNLWNLAFYVFMVFLATLGLWDVFFGFEENKCSMSYMFE
                     YPEYQKIELPKKLAKRYPAYELYLYGEGSYAEEHKILPLTGIPVLFLPGNAGSYKQVR
                     SIGSIALRKAEDIDFKYHFDFFSVNFNEELVALYGGSLQKQTKFVHECIKTILKLYKG
                     QEFAPKSVAIIGHSMGGLVARALLTLKNFKHDLINLLITQATPHVAPVMPLDRFITDF
                     YTTVNNYWILNARHINLTTLSVAGGFRDYQVRSGLTFLPKLSHHTSALSVVSSAVPKT
                     WVSTDHLSIVWCKQLQLTTVRAFFDLIDADTKQITQNSKKKLSVLYHHFIRHPSKHFE
                     ENPAIISDLTGTSMWVLVKVSKWTYVAYNESEKIYFTFPLENHRKIYTHVYCQSTMLD
                     TNSWIFACINSTSMCLQGVDLSWKAELLPTIKYLTLRLQDYPSLSHLVVYVPSVRGSK
                     FVVDCEFFKKEKRYIQLPVTHLFSFGLSSRKVVLNTNGLYYNLELLNFGQIYQAFKIN
                     VVSKCSAVKEEITSIYRLHIPWSYEDSLTIAQAPSSTEISLKLHIAQPENNTHVALFK
                     MYTSSDCRYEVTVKTSFSQILGQVVRFHGGALPAYVVSNILLAYRGQLYSLFSTGCCL
                     EYATMLDKEAKPYKVDPFVIIIKFLLGYKWFKELWDVLLLPELDAVILTCQSMCFPLI
                     SLILFLFGTCTAYWSGLLSSASVRLLSSLWLALKRPSELPKDIKMISPDLPFLTIVLI
                     IVSWTTCGALAILLSYLYYVFKVVHLQASLTTFKNSQPVNPKHSRRSEKKSNHHKDSS
                     IHHLRLSANDAEDSLRMHSTVINLLTWIVLLSMPSLIYWLKNLRYYFKLNPDPCKPLA
                     FILIPTMAILGNTYTVSIKSSKLLKTTSQFPLPLAVGVIAFGSAHLYRLPCFVFIPLL
                     LHALCNFM"
     exon            239..392
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            393..568
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            569..740
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            741..898
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            899..951
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1018
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1124
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1180
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1264
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1311
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1363
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1441
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1517
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1591
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1643
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1710
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1819
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1858
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1952
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2043
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2241
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2377
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2428
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2616
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2721
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..11090
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8486..8491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="hexamer: AATAAA"
     polyA_site      8508
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="major polyA site"
     regulatory      9738..9743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="hexamer: AATAAA"
     polyA_site      9757
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="major polyA site"
     regulatory      11069..11074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
                     /note="hexamer: AATAAA"
     polyA_site      11090
                     /gene="PGAP1"
                     /gene_synonym="Bst1; ISPD3024; MRT42; NEDDSBA; SPG67"
ORIGIN      
        1 agtgtctagc acgctcagtc cgggcttggg gccccgcggc ggagaaggag gtagaggggg
       61 cggcggcggc ggcggcggtg gtggcggcac catgtttctt cactcagtta atctctggaa
      121 cctggcgttt tatgtcttca tggtctttct ggcaaccctg gggctgtggg atgtcttctt
      181 cggcttcgag gagaataagt gcagtatgag ctacatgttt gagtacccgg agtatcagaa
      241 aatagaactt ccaaagaaac tggcaaaacg ctatcccgca tatgagttgt atctttatgg
      301 agagggatcc tatgctgaag aacacaaaat tctccctttg acgggtattc cagttctctt
      361 tcttcctggt aatgctggaa gttataagca agttcgttct attggctcca ttgcacttag
      421 aaaagcagag gacattgact tcaagtacca ctttgacttc tttagtgtga acttcaatga
      481 agaactggtg gctttgtatg gtggaagtct tcagaagcag accaagtttg tacatgaatg
      541 tattaaaaca attctcaaac tctataaggg tcaagaattt gctccaaaaa gtgtggcaat
      601 aattggtcat tctatgggtg gccttgttgc aagagcattg cttacactga aaaattttaa
      661 gcatgatctg ataaatcttc ttattacaca agccacacct catgttgctc ctgtgatgcc
      721 attagatcgt ttcattacag atttttatac gactgtaaac aactattgga ttctaaatgc
      781 tcgacacata aatttaacca cactttctgt agctggagga ttccgggatt accaagttcg
      841 ttcaggattg acttttctac caaaattaag ccatcatacc agtgccttat ctgttgtgag
      901 ttcagcagtg cctaagacct gggtctcaac agaccacctc tccattgtgt ggtgtaaaca
      961 attgcagttg actacagttc gagcattctt tgatcttatt gatgctgata ctaaacaaat
     1021 aactcaaaat tccaagaaga aactgtcagt tttgtatcac cactttataa gacacccatc
     1081 aaaacatttt gaggaaaatc cagctataat ttctgactta acagggacat ctatgtgggt
     1141 tctagtaaaa gtgtccaaat ggacctatgt agcttacaac gaatctgaga agatatattt
     1201 tacatttcct cttgaaaatc atagaaaaat ctacactcat gtctattgtc agagcactat
     1261 gctggataca aatagttgga tttttgcttg cataaacagc acttctatgt gcctgcaagg
     1321 ggttgattta tcatggaaag ctgaactgct gccaacaatt aagtatctga cattaagact
     1381 tcaagactat ccatctttgt ctcatcttgt tgtttatgta ccatctgttc gtggaagtaa
     1441 gtttgttgta gattgtgaat tctttaaaaa agagaaaaga tacatacagc ttcctgtaac
     1501 tcatcttttt tcctttggat tgtcttcaag gaaagtggtg ttaaatacaa atggcctata
     1561 ctacaatcta gagcttctga actttggaca gatataccaa gcttttaaaa tcaacgtggt
     1621 aagcaagtgc tcagcagtca aagaagaaat aaccagtatc tatagacttc atattccttg
     1681 gtcttatgaa gattcactaa ccattgcaca ggctccatct tccacagaaa tttctctgaa
     1741 actccatatt gctcaaccag aaaacaatac ccatgtggca ttatttaaaa tgtacacgtc
     1801 atcagactgt cggtacgagg tgacagttaa gacttccttt tcacaaatac ttggacaggt
     1861 agttagattt catggtggag ctcttcctgc ttatgtcgta tctaatatcc ttcttgctta
     1921 tagaggacag ttatattctc ttttctcaac aggttgttgc ttagaatatg ctaccatgtt
     1981 ggataaagaa gccaaaccat acaaagttga tccttttgta attatcatta agtttctgtt
     2041 ggggtataaa tggtttaaag aattgtggga tgtattattg ttaccagaat tagatgccgt
     2101 cattttaact tgtcagagta tgtgtttccc cctgatatcc ttgattctct ttctgtttgg
     2161 aacgtgtact gcctactgga gtggcctact gtcttctgca tctgtgagac ttctttcttc
     2221 attgtggcta gctttgaaaa gaccctctga acttcctaaa gatatcaaga tgatatcacc
     2281 agacttgccc tttttgacaa ttgtcttgat catagttagt tggacaactt gtggagcact
     2341 agccatactt ctttcttatc tttactatgt gtttaaggtt gttcatctgc aagccagctt
     2401 aacaactttt aagaatagcc agcctgtgaa tcccaaacac tctagaagaa gtgaaaagaa
     2461 atccaatcat cataaagact cctcaataca ccatcttcgt ttatctgcca acgatgctga
     2521 agatagcctt cgcatgcaca gtactgtgat taacttacta acatggattg tattactcag
     2581 catgccttct ctaatttatt ggctaaagaa tcttaggtat tattttaaac ttaatcctga
     2641 tccatgtaaa cctttggcat ttatccttat tccgactatg gcaattcttg gaaatactta
     2701 cactgtttca ataaaatcaa gtaaattgtt gaagactact tcacaatttc cacttcctct
     2761 ggctgttggt gtgattgctt ttgggtcagc acatttatat aggcttccat gctttgtctt
     2821 cattcctctt ttactccatg cattatgcaa ctttatgtaa gattggactt aaggaatgat
     2881 gaagataatt tatgtgttta gggccagtga taagagggaa cacacagatc catcagtatg
     2941 gacagcaaga tcctttggag aagacaagtc tatttttaca atattgaaaa taggaaatta
     3001 gttttgtaat gtttgaggga agtagttgaa gcatggtttt gttttgtggt gtggaatcca
     3061 tgtagtaatc atttttgaaa aattcatgaa gggatatatg gtgatcacta tcattgagga
     3121 ctcctgtgca tataaaatag tctgttttat caactgtacg agaagtctga tatgagagat
     3181 ttagtagatg cagcattatt tgcagtctca ctgcaagcat tctgctcatt tcatcaaact
     3241 ttttttcaca aaagtaggtt attttgaatt tgctatagtt tacctattaa gaaataagtc
     3301 tttaaataac tgatgaaatt tatagctgtt tggtttctca aaggttaaat agccacagaa
     3361 agcctttggt tagtttttgg cagccaccat gaacaaagtg gatcttgtct tcttacatct
     3421 atgaaaatag agctttgaat ggtaaggaga tatgttttct tggtaaccaa tgcaagattg
     3481 atgggtggaa acatgattca aacttacaca atttttcttg ctatttttca aatatgaatc
     3541 ttactatata ttctcggtga acatcaggag actattaaag aggtctgctg ttaaatgtaa
     3601 agaaaaaatg ctcgtagcta tttgcttcct ggtattggag cagttcagtt gtttagttta
     3661 taccattgga ttcaattcat tgcaccatgg ttgccaaaag tgcctgaggt cataatggat
     3721 tgttaaaata actaaattcc agtggttgga aactctaggt ttgtaccatt ttttctgctg
     3781 tgggaaaaaa caacaacaac aacatgatca aggtaacatc acatttgatg tataatatta
     3841 tactattaat ggaatatcag tagacaactg ttaacccatt agtagcatga gtataaacag
     3901 tacacctgaa taaattggag acattagcca ctaggtttaa cagtggaatc ttgatttgcc
     3961 taggtgactt ctgggattac tgtttgacaa ataagagtaa cattttattt catttcagaa
     4021 tttacgtcac ttttagctac aagagtagga agaaggtaat cggcaaggca gaagagtata
     4081 ctctttgcct taggatagcg taaactcagg ctgagacata cccggcttat agagttcttc
     4141 tagatgtgta gactgtaaat gcccaaatcc tctcaactaa agttttagtg attccacaaa
     4201 gcctctcatg taaatttcca gtgattccac cattgcactt gtgaatatgt atccttgtta
     4261 gtacccaggg atgtcctcga gcaccagttt tattttatct gccattgcat ctggattcca
     4321 ttacagcctc tcagctgtta ctgcctgtgg acagttactt ctgcttactg cctgtagaga
     4381 gttacctaac ttctcttctc agttcttcct caggtcctgg ctattttggc ctcagttgaa
     4441 gggagtcttg ctctcatctc tgagggtttt aagtttgttt gatcccattg ttgtcttttc
     4501 tagctttgag catgtttttc agtattcata ttttaactta ctgagaacat taaagggaaa
     4561 tgataaactc gtggtgggga tatggcagac aggtgcttgt ttgtttgaga gaagtagcag
     4621 aagagataaa atacaaagtg ctatatgttt cagctggaga ggaaagagag agaatttatt
     4681 agattatata cttgtcccat ggcataccac gtatatgttt aaatagggac acatctccct
     4741 atgtttaact atacttataa acaactttga tacacattgc gtcttttatt ctgtcacctg
     4801 atattttagt gtatctcaag ttacagatta catgtgtcct taaactattt ctgaatttgg
     4861 acttagttcc atatacagaa agaactttag aaaattcatt aatttggatc ttctattgat
     4921 agccataaat attatgttta tgtattctaa aacctctttg tttagttagt actgttcatg
     4981 aatgtaacaa gcttcaattt ctcatttgtg agtagtacat ttgctttttg tttgtttgtt
     5041 tgtttgtttt tgagatggag tctcacgctg tcaccaggct ggagtgcagt ggcgcgattt
     5101 cagctcactg caacctccac ctcccaggtg caagtgatgc ccctgcctca gcctcccgag
     5161 tagctgggac tacagacacc cgccaccaca cctggctaat ttttgtattt ttagtagaga
     5221 cggggtttca ccatgttggc taggctggtc tcaaactctt gacctcgtga tttgcccgcc
     5281 tctgcctccc caaagtgctg ggattacagg cgtgagccac cacgcccagc cgtacattta
     5341 ctttttaaag cagcagacta ggtacactaa ttctcactca aatattttca tgggaatgta
     5401 gttatcacca agtcctaaag tattatttat gccaaaaaaa atttcatttt aaggactaca
     5461 aaaatgattc taattaaaca ttttataatc aatagtaggt tgggtcttta gccattatat
     5521 gtgtatatat acagacacat atgtatacac ttacattttg acagggtctt cattgagtct
     5581 tgatgcactt taaacccagc tggctaccag agatgcgaag gtgggctctt tgaagattag
     5641 caaaatggac gtttctgtca cttgagaaaa ggaaagttct ttgcctttaa attacacagt
     5701 tttcatcatg cccacaatct atattattgg ctggttaaac agcactgccc tattagcaat
     5761 gttaacaaaa atgaaattat ttattggcgg ttatagatta tctaattcag gaaatttctg
     5821 agctcaactt ttacagcaac tgttatgcct tctaatttag caattgagtt atgagtaagt
     5881 tttgtgctta actcctagac cctattgttg ataaccagat caaatatagt ctgtacagag
     5941 gaaaacactg ggaacattta gtatttctaa agcctccttt ggagttacta ctgattgtaa
     6001 tttggaactg ataataggta gagattgcta acactgtttt ttttcctgga tcttttttat
     6061 gccagaaatt aaacaggttc tgctaactct tttttttctc ttggttatca ccagaatgaa
     6121 aatatttaaa gtgatgactc tagaaaagcc atctgtgcct ggttaacatt gagtttgagt
     6181 ctcttcaata tatattgatc atgtattgat taatctttat tttttcatat tttggctaga
     6241 caaattcaga tctatataat ggaatacccc ttcttgagtg aactatacta ctaatctaca
     6301 tgattatata gtaaggaaaa aagaagaaat aactgtaata ggcatagtgt ttgttgttgg
     6361 ttgtcttgtc attcatgtga tactactcat ttccaaaatt cacacaaact tacatgaggt
     6421 ggattatttg ttttgttcat tatttagttc ctatatgttt tttctttaga aacagagtct
     6481 cattctgtca cccaggctgg agtccaatgg ggcggtcata gttcactgca gccttgaact
     6541 ctttggctca tgtgatcctc ccatctcagc ctcccacagc aggtgagact acaggtacat
     6601 gccactgtgc ctgacttttt aatttttttg tagagacgag gtttcagttt gttgcccaag
     6661 ctgatcttga actcctggtt tcaagcgatc ctcccacctc ggcctcccaa agtagtggga
     6721 tttcaggcat gaccacctgg cctagttcct atacttttct taattcttca gacttctcac
     6781 atttagtata gtgcattcat ttcatcttgc tgtttattag caccctttgt ggccaaggga
     6841 aataaaaggt ggtaaaattc agttttcagt ttagttcttg aaagctctgg gaaatggagg
     6901 aaacacaaaa ctatgaatta aactagggct gttgatttct gaacccccag ataaatcagt
     6961 tgacccacat tttcatttta ggtgttaggt ccaaattagc ataatgtctt gcattattat
     7021 taggttcagt gtgaaacttt acagtgctgc atttgaagtt tagtaactgg ttattattaa
     7081 tcatttggga aaaatgaaaa tgtgttggga ctttctatga ctaggcattt gttgattatt
     7141 tttcatgatt gctttttgtt ttctcattgt gtaggatttg tgaacttgta tattacagga
     7201 aacaagatac tttgtaaaat ttactgggga aaatccattt ggagtgcatg acatttgcca
     7261 ggataagaaa gcagtaatat gtttgtatta taaaattaca ccctgccaga aaactttctt
     7321 tcctagtaag gtaaatgtag aagggacttt tacagcatag taagttgatt aggagccaaa
     7381 attttattcc agtttttttt tgaactaaga atgttttaaa ttctgtaatg aacttttatg
     7441 tttacccatt actcatgcat tctttcacaa tatgtttaat agcctgagga aataggaaag
     7501 ctgtgaagct actaccattc tttactttta ataagaataa taggaaagaa aagtcaggtc
     7561 agtaatccaa atccaaatat gtatactgca aatgctcaag aagtcacatt ttttgataaa
     7621 ttgtattgag tacagaagaa cttatatgaa tttattatct gttaataact tagttttgac
     7681 aacagaataa catttggaaa ttgtgagaat aatcaagctg tttttccatt aacagtgtaa
     7741 attcataaca tgtccttcaa aaggtgatat tctaagctgt cttaattgtc tacggttgat
     7801 aacttttaaa taaagtacag gactttctga aagtgtttgg catgttatgc tgccaaaaac
     7861 aatctgtgtt ttgaaatacc aattaatcag ttaatttctg aagactttgt ataggacttg
     7921 atatatgagt cagaatctgt ctgtactcat tctgtacatt gtaactttga acacttatga
     7981 aaaactgtat ctgttggtgt gttttgatta gttagtgtag atttgtttgc gtatttgaat
     8041 tccgatttta gtttaggaag actaaaagta gccatttttg taaagttcat atgctatttt
     8101 ttaatgtcat ttttgttttt aatatttata caatagtgat gttactagta aaaaatgttt
     8161 atagataaca cgtagagcta ttaactgttc aaaagcctac atgataggca tattttgtat
     8221 ttcgtgttgc actcgttctg tttcatattg gactttttac atcccttttt tagcaaaaaa
     8281 aagagacaca tttgaattct ctttagcata aagctgtgca ttggaaacta tgtgactgta
     8341 tccatacggt tagcaaaata ctctttgcca ccaaaggtaa atgaaactgt aaaatacctc
     8401 tggatatttg tgccaatgaa cttttcttag catattagga ttaaagcaaa aataatcttt
     8461 tcagtatgtt tcatctagga cttacaataa atgtttaaac catgaaaact tatatttcat
     8521 accctaattt ttctttgaaa atattcaaga tttgtagcct agtgtgttct attttgtgtg
     8581 attgcttgaa tgttttctga ttttattggg taaaagtttg cctagtaatg atctttcagc
     8641 aattcattga aataattatt tgaagtaatc ttgcttcaga tttaatttgt gatgtacatt
     8701 gttgtacaag ccattttagt acaggtgtct catttatgta gttgaaaggt gactgtggaa
     8761 tttcttttgt tttttatgta tttccattca gtctctttat atagtcacat gtttcacagg
     8821 ggaacttgtc cactggaacc taacatagta caacctttta atatgcgtat cttaaaggaa
     8881 aatattataa aacaagatat taatactaaa gtgttaacat taatatgttg ttctcatgga
     8941 agaaaagtag aaaatgtgat aggcttagaa attatttttt atcagcatta agtgcttcaa
     9001 tttctcccca taaagattct aaggaaattt cagttcctca tattatagtt ttccccataa
     9061 tttaatatta ctaagtattt ctctgcccag taatgttgat gcagtttgca taaatagcct
     9121 tggaagtaag gaggcaggac agaaagccaa atatcgaaat ctctggcctt gatttagtga
     9181 cagtttattc taatggggac cataggtgtt attagtaaaa agatagtgta caaggcctaa
     9241 gttcagttta cattgttctt tgaaatgagt tcatcttttg tgttgaataa ttgtattcta
     9301 agtaggagat gcctgtattt aacataatca tgctttctat ataatcaaat atgtatttgt
     9361 tggaatactg gtagaaataa ccttcccttc ctccactgtg cagtggaaaa aaaactcccg
     9421 atttatactg tatataaatt aggaaattat gttacatatt tacattttta aaaatataaa
     9481 aatgacatat tattttgaag tgattgtatt atagtttctg aagcttatga ctggctataa
     9541 atataacaac atacccatga gaaaccagac caaaattgaa tatatatcat cactgtgacc
     9601 ttgaacttat agtcatttgg gataaaacag agtgtgcctt gaaatgataa agagtgccct
     9661 gcaattttat aactgtacat tttattatgg gtggtcctct caaattggcc ccaaatcctt
     9721 gtcttcgagg ttggtgaaat aaaagtttaa aataaaagtt gactggtatt tcctttagta
     9781 tttccagaac tattgctgta gaggcctccc ttaattttca gagttctcat tttgaattaa
     9841 agtagactta cgctacatag atgtagaaaa ttaggtttgg cttaggtttc tgaaaaaata
     9901 ttctaggaga aggtggtagc attgttctga acttaccaca cttggaaaaa ctaatcttca
     9961 aatgtaattt tttgtcttat taatgagtaa tctgtcaaag acatgaattc tttgacttgt
    10021 gctcattctt tctgaactta ttgaggctta tatatatcaa ataaggttaa tttacacaat
    10081 aattattttt aataattaat aaactcattc atgtagtata ttttaaaaca ttatttcctc
    10141 tcatttttcc ttctcttttt tcttcctata tatttttaag gcttcaagag aaaacgggct
    10201 tcagttttta aaaaaatgta gacttgcatt tttactgtgc ttatttaaaa taatggatat
    10261 aatttatatt aaggtccaaa ttattttatt cacaggataa tcatggtctc tgataaaaga
    10321 tgcccacatt tctgtgtact cacagttgtt gtatattttt caatttcaat tttgaaagta
    10381 aactcaacac attgttttcc cattttacct ccactaccta ctcgccagac aaaaatatag
    10441 atttctctaa aaacacatac aaagaaaatg tctttcctta aaacatgatc ttattgacat
    10501 gaaggtagtt tgagcaaggg tatgtaataa aataaatggc tctcaacctg catcaatttt
    10561 tatactatag ttaaaaaatt tatagaggac aacctatgtg ttatgctgag atagactcag
    10621 taatcatttg tttgcaaatt gattattttt ttctcatcca tccagaaagt acagtgcata
    10681 acaacaacac ttaacaagtg cagaggaacc tgtcctgtag tatataataa aaaatgattg
    10741 tcaccttgct gatctttcca tttaaacatg catgatctcc agtactttgt acaatgtata
    10801 gtatattaaa tacatttatg tgtaattttg tgtgtatttt tatatggtat aattaaaatt
    10861 attacattta tgtgattgta attcacaaaa agtactactt actatattct gtagagtgtg
    10921 ttgtgaatgt ttttacctgt gtttgagcat tatggtgtag gtttaataga tgaatatttg
    10981 ccttcagtct ctgctcaggt attaatgtgt agtaattttt ggtgctttct ttttaatctg
    11041 tgataatctg gtgttctgaa tgttcaaaaa taaatttggt tgaatacgca
//
