LOCUS       NM_014224               1382 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens pepsinogen A5 (PGA5), mRNA.
ACCESSION   NM_014224
VERSION     NM_014224.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Guo,Z., Wu,Y., Chen,J., Zhang,S. and Zhang,C.
  TITLE     The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal
            Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and
            24-Hour Multichannel Intraluminal Impedance-pH Monitoring
  JOURNAL   Med Sci Monit 26, e927381 (2020)
   PUBMED   33220027
  REMARK    GeneRIF: The Role of Salivary Pepsin in the Diagnosis of
            Gastroesophageal Reflux Disease (GERD) Evaluated Using
            High-Resolution Manometry and 24-Hour Multichannel Intraluminal
            Impedance-pH Monitoring.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Samuels,T.L., Altman,K.W., Gould,J.C., Kindel,T., Bosler,M.,
            MacKinnon,A., Hagen,C.E. and Johnston,N.
  TITLE     Esophageal pepsin and proton pump synthesis in barrett's esophagus
            and esophageal adenocarcinoma
  JOURNAL   Laryngoscope 129 (12), 2687-2695 (2019)
   PUBMED   31046139
  REMARK    GeneRIF: Local synthesis of pepsin and proton pumps in BE and EAC
            is not uncommon. Absence of these molecules in normal (noncancer)
            esophagi, SCC, and in vitro data support a role for pepsin in
            reflux-attributed carcinogenic changes in the esophagus
REFERENCE   3  (bases 1 to 1382)
  AUTHORS   Terasawa,T., Nishida,H., Kato,K., Miyashiro,I., Yoshikawa,T.,
            Takaku,R. and Hamashima,C.
  TITLE     Prediction of gastric cancer development by serum pepsinogen test
            and Helicobacter pylori seropositivity in Eastern Asians: a
            systematic review and meta-analysis
  JOURNAL   PLoS One 9 (10), e109783 (2014)
   PUBMED   25314140
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1382)
  AUTHORS   Zhang,X.M., Li,J.X., Zhang,G.Y., Li,X.H. and Gu,H.
  TITLE     The value of serum pepsinogen levels for the diagnosis of gastric
            diseases in Chinese Han people in midsouth China
  JOURNAL   BMC Gastroenterol 14, 3 (2014)
   PUBMED   24383519
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1382)
  AUTHORS   Kageyama,T.
  TITLE     Pepsinogens, progastricsins, and prochymosins: structure, function,
            evolution, and development
  JOURNAL   Cell Mol Life Sci 59 (2), 288-306 (2002)
   PUBMED   11915945
  REMARK    Review article
REFERENCE   6  (bases 1 to 1382)
  AUTHORS   Takahashi,K.
  TITLE     Gene structures of pepsinogens A and C
  JOURNAL   Scand J Clin Lab Invest Suppl 210, 97-110 (1992)
   PUBMED   1455184
  REMARK    Review article
REFERENCE   7  (bases 1 to 1382)
  AUTHORS   Ichinose,M., Miki,K., Wong,R.N., Tatematsu,M., Furihata,C.,
            Konishi,T., Matsushima,M., Tanji,M., Sano,J., Kurokawa,K. et al.
  TITLE     Methylation and expression of human pepsinogen genes in normal
            tissues and their alteration in stomach cancer
  JOURNAL   Jpn J Cancer Res 82 (6), 686-692 (1991)
   PUBMED   1906854
REFERENCE   8  (bases 1 to 1382)
  AUTHORS   ten Dam,M.A., van Kamp,G.J., Kok,A., Meuwissen,S.G., Donker,A.J.
            and ten Kate,R.W.
  TITLE     The effect of acute and chronic protein loading on urinary
            pepsinogen A excretion
  JOURNAL   Nephron 59 (2), 239-243 (1991)
   PUBMED   1956484
REFERENCE   9  (bases 1 to 1382)
  AUTHORS   Meijerink,P.H., Bebelman,J.P., Pals,G., Arwert,F., Planta,R.J.,
            Eriksson,A.W. and Mager,W.H.
  TITLE     Analysis of the promoter of a human pepsinogen A gene
  JOURNAL   Adv Exp Med Biol 306, 87-90 (1991)
   PUBMED   1812762
REFERENCE   10 (bases 1 to 1382)
  AUTHORS   Sogawa,K., Fujii-Kuriyama,Y., Mizukami,Y., Ichihara,Y. and
            Takahashi,K.
  TITLE     Primary structure of human pepsinogen gene
  JOURNAL   J Biol Chem 258 (8), 5306-5311 (1983)
   PUBMED   6300126
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003037.2.
            
            On Nov 21, 2019 this sequence version replaced NM_014224.4.
            
            Summary: This gene encodes a protein precursor of the digestive
            enzyme pepsin, a member of the peptidase A1 family of
            endopeptidases. The encoded precursor is secreted by gastric chief
            cells and undergoes autocatalytic cleavage in acidic conditions to
            form the active enzyme, which functions in the digestion of dietary
            proteins. This gene is found in a cluster of related genes on
            chromosome 11, each of which encodes one of multiple pepsinogens.
            Pepsinogen levels in serum may serve as a biomarker for atrophic
            gastritis and gastric cancer. [provided by RefSeq, Jul 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029055.1, SRR18074967.3370298.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312403.10/ ENSP00000309542.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AP003037.2         21160-21267
            109-271             AP003037.2         21754-21916
            272-389             AP003037.2         24150-24267
            390-508             AP003037.2         25137-25255
            509-708             AP003037.2         25931-26130
            709-825             AP003037.2         28404-28520
            826-970             AP003037.2         29654-29798
            971-1069            AP003037.2         29901-29999
            1070-1382           AP003037.2         31117-31429
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.2"
     gene            1..1382
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /note="pepsinogen A5"
                     /db_xref="GeneID:5222"
                     /db_xref="HGNC:HGNC:8887"
                     /db_xref="MIM:169730"
     exon            1..108
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1219
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /EC_number="3.4.23.1"
                     /note="pepsin A; pepsinogen-5; pepsin A-5; Pepsin A-4;
                     Pepsinogen-4; pepsinogen 5, group I (pepsinogen A)"
                     /codon_start=1
                     /product="pepsin A-5 preproprotein"
                     /protein_id="NP_055039.1"
                     /db_xref="CCDS:CCDS8001.1"
                     /db_xref="GeneID:5222"
                     /db_xref="HGNC:HGNC:8887"
                     /db_xref="MIM:169730"
                     /translation="MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKK
                     HNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWV
                     PSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTN
                     QIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLS
                     ADDKSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGETIACAEGCQAIV
                     DTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPS
                     AYILQSEGSCISGFQGMNVPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA"
     exon            109..271
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            272..389
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            390..508
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            509..708
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            709..825
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            826..970
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1069
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1382
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1360..1365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /note="hexamer: AATAAA"
     polyA_site      1382
                     /gene="PGA5"
                     /gene_synonym="Pg5"
                     /note="major polyA site"
ORIGIN      
        1 gcaccttcct cccatcttgc cttctccctc gagttgggac ccgggaagaa ccatgaagtg
       61 gctgctgctg ctgggtctgg tggcgctctc tgagtgcatc atgtacaagg tccccctcat
      121 cagaaagaag tccttgaggc gcaccctgtc cgagcgtggc ctgctgaagg acttcctgaa
      181 gaagcacaac ctcaacccag ccagaaagta cttcccccag tgggaggctc ccaccctggt
      241 agatgaacag cccctggaga actacctgga tatggagtac ttcggcacta tcggcatcgg
      301 aactcctgcc caggatttca ccgtcgtctt tgacaccggc tcctccaacc tgtgggtgcc
      361 ctcagtctac tgctccagtc ttgcctgcac caaccacaac cgcttcaacc ctgaggattc
      421 ttccacctac cagtccacca gcgagacagt ctccatcacc tacggcaccg gcagcatgac
      481 aggcatcctc ggatacgaca ctgtccaggt tggaggcatc tctgacacca atcagatctt
      541 cggcctgagc gagacggaac ctggctcctt cctgtattat gctcccttcg atggcatcct
      601 ggggctggcc taccccagca tttcctcctc cggggccaca cccgtctttg acaacatctg
      661 gaaccagggc ctggtttctc aggacctctt ctctgtctac ctcagcgccg atgacaagag
      721 tggcagcgtg gtgatctttg gtggcattga ctcttcttac tacactggaa gtctgaactg
      781 ggtgcctgtt accgtcgagg gttactggca gatcaccgtg gacagcatca ccatgaacgg
      841 agagaccatc gcctgtgctg agggctgcca ggccattgtt gacaccggca cctctctgct
      901 gaccggccca accagcccca ttgccaacat ccagagcgac atcggagcca gcgagaactc
      961 agatggcgac atggtggtca gctgctcagc catcagcagc ctgcccgaca tcgtcttcac
     1021 catcaatgga gtccagtacc ccgtgccacc cagtgcctac atcctgcaga gcgaggggag
     1081 ctgcatcagt ggcttccagg gcatgaacgt ccccaccgaa tctggagagc tttggatcct
     1141 gggtgatgtc ttcatccgcc agtactttac cgtcttcgac agggcaaaca accaggtcgg
     1201 cctggcccct gtggcttaag cctaagtctc ttcagccacc tcccaggaag atctggcctc
     1261 cgtcctatgc ccactttaga tgtatctaat tctcctgact gttcttccca ggggagtgtg
     1321 aaggtcttgg ccctgttccc tgtcctacca ataacgtaga ataaaaacat aacccactga
     1381 aa
//
