LOCUS       NM_004567               3499 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
            (PFKFB4), transcript variant 2, mRNA.
ACCESSION   NM_004567
VERSION     NM_004567.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Liu,Y., Shi,Q., Liu,Y., Li,X., Wang,Z., Huang,S., Chen,Z. and He,X.
  TITLE     Fibrillarin reprograms glucose metabolism by driving the
            enhancer-mediated transcription of PFKFB4 in liver cancer
  JOURNAL   Cancer Lett 602, 217190 (2024)
   PUBMED   39182558
  REMARK    GeneRIF: Fibrillarin reprograms glucose metabolism by driving the
            enhancer-mediated transcription of PFKFB4 in liver cancer.
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Chen,R., Gao,X. and Shen,Y.
  TITLE     MiR-3195 inhibits non-small cell lung cancer malignant behaviors
            along with cisplatin resistance through targeting PFKFB4
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 73-78 (2024)
   PUBMED   39097893
  REMARK    GeneRIF: MiR-3195 inhibits non-small cell lung cancer malignant
            behaviors along with cisplatin resistance through targeting PFKFB4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3499)
  AUTHORS   Chen,S., Han,J., Wang,W., Wu,Z., Wang,J. and Cheng,B.
  TITLE     Expression and prognostic analysis of PFKFB4 in oral squamous cell
            carcinoma
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 193-199 (2024)
   PUBMED   39097875
  REMARK    GeneRIF: Expression and prognostic analysis of PFKFB4 in oral
            squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3499)
  AUTHORS   Altunok,T.H., Muchut,R.J., Iglesias,A.A. and Yalcin,A.
  TITLE     Transforming growth factor beta1 upregulates
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-4 expression
            in A549 and MCF-10A cells
  JOURNAL   Cell Biochem Funct 41 (8), 1220-1229 (2023)
   PUBMED   37707291
  REMARK    GeneRIF: Transforming growth factor beta1 upregulates
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-4 expression
            in A549 and MCF-10A cells.
REFERENCE   5  (bases 1 to 3499)
  AUTHORS   Yu,T., Zhang,Q., Yu,S.K., Nie,F.Q., Zhang,M.L., Wang,Q. and Lu,K.H.
  TITLE     THOC3 interacts with YBX1 to promote lung squamous cell carcinoma
            progression through PFKFB4 mRNA modification
  JOURNAL   Cell Death Dis 14 (7), 475 (2023)
   PUBMED   37500615
  REMARK    GeneRIF: THOC3 interacts with YBX1 to promote lung squamous cell
            carcinoma progression through PFKFB4 mRNA modification.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3499)
  AUTHORS   Manzano,A., Perez,J.X., Nadal,M., Estivill,X., Lange,A. and
            Bartrons,R.
  TITLE     Cloning, expression and chromosomal localization of a human testis
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene
  JOURNAL   Gene 229 (1-2), 83-89 (1999)
   PUBMED   10095107
REFERENCE   7  (bases 1 to 3499)
  AUTHORS   Hirata,T., Kato,M., Okamura,N., Fukasawa,M. and Sakakibara,R.
  TITLE     Expression of human placental-type
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various
            cells and cell lines
  JOURNAL   Biochem Biophys Res Commun 242 (3), 680-684 (1998)
   PUBMED   9464277
REFERENCE   8  (bases 1 to 3499)
  AUTHORS   Sakai,A., Kato,M., Fukasawa,M., Ishiguro,M., Furuya,E. and
            Sakakibara,R.
  TITLE     Cloning of cDNA encoding for a novel isozyme of fructose
            6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human
            placenta
  JOURNAL   J Biochem 119 (3), 506-511 (1996)
   PUBMED   8830046
REFERENCE   9  (bases 1 to 3499)
  AUTHORS   Pilkis,S.J., Claus,T.H., Kurland,I.J. and Lange,A.J.
  TITLE     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic
            signaling enzyme
  JOURNAL   Annu Rev Biochem 64, 799-835 (1995)
   PUBMED   7574501
  REMARK    Review article
REFERENCE   10 (bases 1 to 3499)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB069657.1 and BC010269.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004567.3.
            
            Summary: The protein encoded by this gene is one of four
            bifunctional kinase/phosphatases that regulate the concentration of
            the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The
            encoded protein is highly expressed in cancer cells and is induced
            by hypoxia. This protein is essential to the survival of cancer
            cells under conditions of hypoxia, because it increases the amount
            of F2,6BP and ATP at a time when the cell cannot produce much of
            them. This finding suggests that this protein may be a good target
            for disruption in cancer cells, hopefully imperiling their
            survival. Several transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (2) lacks an alternate coding exon
            in the 5' end compared to variant 1. The resulting isoform (b) has
            a shorter and distinct N-terminus compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010269.1, SRR1660805.63806.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000232375.8/ ENSP00000232375.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DB069657.1         121-129
            10-3499             BC010269.1         1-3490
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..3499
                     /gene="PFKFB4"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     4"
                     /db_xref="GeneID:5210"
                     /db_xref="HGNC:HGNC:8875"
                     /db_xref="MIM:605320"
     exon            1..123
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1436
                     /gene="PFKFB4"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform b is encoded by transcript variant 2;
                     bifunctional enzyme with kinase and biphosphatase
                     activities; 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase
                     testis-type isozyme;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4;
                     PFK/FBPase 4"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 4 isoform b"
                     /protein_id="NP_004558.1"
                     /db_xref="CCDS:CCDS2771.1"
                     /db_xref="GeneID:5210"
                     /db_xref="HGNC:HGNC:8875"
                     /db_xref="MIM:605320"
                     /translation="MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVM
                     VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKI
                     RKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICV
                     DPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKI
                     MDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSP
                     RGREFAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEE
                     MTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQ
                     AVMRCLLAYFLDKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQ
                     NVDISRPPEEALVTVPAHQ"
     exon            124..240
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            241..337
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            338..404
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            405..479
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            480..536
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            537..658
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            659..866
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1013
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1118
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1248
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1311
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1376
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..3499
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3477..3482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFKFB4"
                     /note="hexamer: AATAAA"
     polyA_site      3499
                     /gene="PFKFB4"
                     /note="major polyA site"
ORIGIN      
        1 gcagtccgac tcatcccggc cccgggatgg cgtccccacg ggaattgaca cagaaccccc
       61 tgaagaagat ctggatgcca tacagcaatg ggcggcccgc tctgcacgct tgccagcgcg
      121 gtgtgtgcat gaccaactgc ccaactctca ttgtcatggt gggcctgccc gccaggggca
      181 agacctacat ctccaagaag ctgactcgat acctgaactg gattggtgtg cccactcggg
      241 agttcaatgt tggccagtat cgccgggacg tggtcaagac ctacaaatct tttgaatttt
      301 ttctccccga caatgaagag ggcctgaaaa tcaggaagca gtgtgccctg gcagccctcc
      361 gtgacgtccg gcggttcctt agtgaggagg ggggacatgt ggcggttttt gatgccacaa
      421 acaccacccg agaacggaga gcgaccatct ttaattttgg agaacagaat ggctacaaga
      481 ccttttttgt cgagtccatc tgtgtggatc ctgaggtcat agctgccaac atcgtgcaag
      541 tgaaactggg cagccctgac tatgtcaacc gcgacagtga tgaggctacg gaggacttca
      601 tgaggcgcat tgagtgctat gagaactcct acgagtcgct agatgaggac ctggataggg
      661 acctgtccta tatcaagatc atggatgtgg gccagagcta cgtggtgaac cgtgtggctg
      721 accacatcca gagccgcatc gtatattacc tcatgaacat ccacgtgacc ccccgctcca
      781 tctacctctg ccggcacggg gagagcgagc tcaacctcaa gggccggatt ggcggggacc
      841 caggactgtc ccctcggggc agggagtttg ccaagagtct agcccagttc atcagtgacc
      901 aaaatatcaa ggatctgaag gtctggacaa gccagatgaa gaggacaatc cagacggctg
      961 aggcactggg tgtgccctat gaacagtgga aggtcctcaa cgagatcgat gcgggcgtct
     1021 gtgaggaaat gacctacgag gaaattcagg ataattatcc actggagttc gccctgcggg
     1081 accaggacaa gtaccggtac cggtacccta aaggggagtc ctacgaggac ctggtccaga
     1141 gactggagcc tgtcatcatg gagctggaga ggcaagagaa tgtgctggtc atctgccacc
     1201 aggctgtgat gcgctgcctg ctggcctact tcctcgacaa ggcagcagaa cagctgccct
     1261 acctcaagtg tccgctgcac acagtcctga agctgactcc tgtggcatat ggttgtaaag
     1321 tggagtccat attcctgaac gtggctgctg tgaacacgca ccgggacagg cctcagaacg
     1381 tggacatctc aagacctcca gaggaagccc ttgtcacggt gcctgctcac cagtgaccat
     1441 gttcatccac tgtgaccact aggcaggcac tgctctctgc agagggggtc attccaggcc
     1501 ctccagtgtg tgtgatagtc accatgccat gcagggatat tcttgaagcc acacatggct
     1561 ggcggaaccc agagccccca ccccagccca cctggctctt tgttgacagt cggcgacaag
     1621 gttgtgcgtg gctcctgacc tgctgctaag agtcacttga ccagactgca tctgcatggg
     1681 ctgcgcggag gttgcccagc cccagtttct tccggcgcag ctcttaggtg ttcactctcg
     1741 ccagctcagt tggctttgtg aagtgtgaaa ccctacaatg tgaaaggaaa gtgcttgctg
     1801 tgatgttcct actgtggccc agctgcccag catggacctg gtgactctcc acagggcctc
     1861 taccatcctc tctgtggcca cttcctgagc cagaggccag gtcttcatgg ggccctgagc
     1921 ttctgctgcc tctggtgaga gggagagccc ttcccatcct tacccaccag gaactagagc
     1981 cccaaccaca gcagatgctt cctgggcagc cactggccag gccgttgtat ccatgtcacc
     2041 cttagttgtg ggcattcatg aaagcaatgc gcttgcttca gcacattggg atgcacagaa
     2101 acgtgagggc aggggggcag ttgtggtcgc ccctccccag ttgtgcatgt acccggctac
     2161 ttaggtatgc ttaggggagc tggtggccag gggtgcccct tcaggcctgc tctcacagtg
     2221 aaaccacttc aagacagggc aggaagttac cttcctggtg cacacaccga agtgctcgag
     2281 ggagaggctg ccgccccctc tccccttccc tggctgtgga cactggcagc caatggggag
     2341 gcggcctctc ccctgttgct gcccgtgtcc acacaggaat gtacactggt ggcagaaaca
     2401 tgcatttgcc ataaataatt cagaaacact tgtatggtcc tgtaaaactt ctctggtgtt
     2461 tgttgacttc tgcctgtggg tctgaggaag ggagaaggca gaacccatag gcagtccctg
     2521 ggcaagggag gattttcccg gccgcagcac agccttccat tcatgtgcac gtgagtctgg
     2581 tgaaaatggc cttgtgaagt ccagtgtctg tcatgcccct gagttctttt ggtcccagag
     2641 aggcagcctg ggcagtctgt ttacagctgg caaacagact ggctggcaag ttgtggggct
     2701 gggtcagaaa gtgtctggac ttgtaatcgg gtgccctgct gtccacaggt ggactctggg
     2761 gaagccaggc ccctggatcc caagtccttt gtgtccggag cctttcctgg cctgtgcctg
     2821 gagggtagca catctttcca gcctcccagg gctttgcagc ctgcagatgc cacctgtccc
     2881 tgagagaagt tagcgtccct ggccctggcc ggtggtctct catccttgtg tgctgctctg
     2941 ctaagagatg tccaaggcgg agccggggca agatccttcc agactcatct gtcagagccc
     3001 caagcccttt agacccagag cccaaggacc atgcctttgg gacattagga ctgcagcctt
     3061 tgcttctgtg tattttggag ttttggtgac ttttgtcacc tggacacact catttgttag
     3121 ccatagtggg ttcccttggt cagcaacagt gcatgtacct ctggatgtca tctgaggtga
     3181 gaccaccgag gccttttctc tctgtgtaca gaggggagtt aggagttgct ggactggatg
     3241 cattacgagg actggggaca gggtagaggg acatccaggg atcagggcat gagtgggggc
     3301 aaccccccgg cctctgccct ggcatggtct ccgcatgggc tgaggtgtag ctgattggct
     3361 gccacatttc ggccatgctg gctggcgtgc ccatgttgca gatattttcc cgagttcccc
     3421 agaatggatg gtattgaatc tcagccacat gcaacactgt gtccagcatt ctttgcaata
     3481 aatacttttt aaaaaataa
//
