LOCUS       NM_001317134            3647 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
            (PFKFB4), transcript variant 1, mRNA.
ACCESSION   NM_001317134
VERSION     NM_001317134.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Liu,Y., Shi,Q., Liu,Y., Li,X., Wang,Z., Huang,S., Chen,Z. and He,X.
  TITLE     Fibrillarin reprograms glucose metabolism by driving the
            enhancer-mediated transcription of PFKFB4 in liver cancer
  JOURNAL   Cancer Lett 602, 217190 (2024)
   PUBMED   39182558
  REMARK    GeneRIF: Fibrillarin reprograms glucose metabolism by driving the
            enhancer-mediated transcription of PFKFB4 in liver cancer.
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Chen,R., Gao,X. and Shen,Y.
  TITLE     MiR-3195 inhibits non-small cell lung cancer malignant behaviors
            along with cisplatin resistance through targeting PFKFB4
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 73-78 (2024)
   PUBMED   39097893
  REMARK    GeneRIF: MiR-3195 inhibits non-small cell lung cancer malignant
            behaviors along with cisplatin resistance through targeting PFKFB4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3647)
  AUTHORS   Chen,S., Han,J., Wang,W., Wu,Z., Wang,J. and Cheng,B.
  TITLE     Expression and prognostic analysis of PFKFB4 in oral squamous cell
            carcinoma
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 193-199 (2024)
   PUBMED   39097875
  REMARK    GeneRIF: Expression and prognostic analysis of PFKFB4 in oral
            squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3647)
  AUTHORS   Altunok,T.H., Muchut,R.J., Iglesias,A.A. and Yalcin,A.
  TITLE     Transforming growth factor beta1 upregulates
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-4 expression
            in A549 and MCF-10A cells
  JOURNAL   Cell Biochem Funct 41 (8), 1220-1229 (2023)
   PUBMED   37707291
  REMARK    GeneRIF: Transforming growth factor beta1 upregulates
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-4 expression
            in A549 and MCF-10A cells.
REFERENCE   5  (bases 1 to 3647)
  AUTHORS   Yu,T., Zhang,Q., Yu,S.K., Nie,F.Q., Zhang,M.L., Wang,Q. and Lu,K.H.
  TITLE     THOC3 interacts with YBX1 to promote lung squamous cell carcinoma
            progression through PFKFB4 mRNA modification
  JOURNAL   Cell Death Dis 14 (7), 475 (2023)
   PUBMED   37500615
  REMARK    GeneRIF: THOC3 interacts with YBX1 to promote lung squamous cell
            carcinoma progression through PFKFB4 mRNA modification.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3647)
  AUTHORS   Manzano,A., Perez,J.X., Nadal,M., Estivill,X., Lange,A. and
            Bartrons,R.
  TITLE     Cloning, expression and chromosomal localization of a human testis
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene
  JOURNAL   Gene 229 (1-2), 83-89 (1999)
   PUBMED   10095107
REFERENCE   7  (bases 1 to 3647)
  AUTHORS   Hirata,T., Kato,M., Okamura,N., Fukasawa,M. and Sakakibara,R.
  TITLE     Expression of human placental-type
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various
            cells and cell lines
  JOURNAL   Biochem Biophys Res Commun 242 (3), 680-684 (1998)
   PUBMED   9464277
REFERENCE   8  (bases 1 to 3647)
  AUTHORS   Sakai,A., Kato,M., Fukasawa,M., Ishiguro,M., Furuya,E. and
            Sakakibara,R.
  TITLE     Cloning of cDNA encoding for a novel isozyme of fructose
            6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human
            placenta
  JOURNAL   J Biochem 119 (3), 506-511 (1996)
   PUBMED   8830046
REFERENCE   9  (bases 1 to 3647)
  AUTHORS   Pilkis,S.J., Claus,T.H., Kurland,I.J. and Lange,A.J.
  TITLE     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic
            signaling enzyme
  JOURNAL   Annu Rev Biochem 64, 799-835 (1995)
   PUBMED   7574501
  REMARK    Review article
REFERENCE   10 (bases 1 to 3647)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY714243.1, AC134772.2 and
            BC010269.1.
            
            On May 31, 2019 this sequence version replaced NM_001317134.1.
            
            Summary: The protein encoded by this gene is one of four
            bifunctional kinase/phosphatases that regulate the concentration of
            the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The
            encoded protein is highly expressed in cancer cells and is induced
            by hypoxia. This protein is essential to the survival of cancer
            cells under conditions of hypoxia, because it increases the amount
            of F2,6BP and ATP at a time when the cell cannot produce much of
            them. This finding suggests that this protein may be a good target
            for disruption in cancer cells, hopefully imperiling their
            survival. Several transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY714243.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               AY714243.1         88-269
            183-189             AC134772.2         169846-169852       c
            190-1657            AY714243.1         277-1744
            1658-3647           BC010269.1         1501-3490
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..3647
                     /gene="PFKFB4"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     4"
                     /db_xref="GeneID:5210"
                     /db_xref="HGNC:HGNC:8875"
                     /db_xref="MIM:605320"
     exon            1..123
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1584
                     /gene="PFKFB4"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform a is encoded by transcript variant 1;
                     bifunctional enzyme with kinase and biphosphatase
                     activities; 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase
                     testis-type isozyme;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4;
                     PFK/FBPase 4"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 4 isoform a"
                     /protein_id="NP_001304063.1"
                     /db_xref="GeneID:5210"
                     /db_xref="HGNC:HGNC:8875"
                     /db_xref="MIM:605320"
                     /translation="MGGPLCTLASAVNFQRWASFPTGGRVPLGLKAELGQELQQRCAQ
                     DRDRKVCRVGLCLCPGTLCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREF
                     NVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSEEGGHVAVFDAT
                     NTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATE
                     DFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHV
                     TPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVWTSQMK
                     RTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKG
                     ESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVL
                     KLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ"
     exon            124..271
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            272..388
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            389..485
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            486..552
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            553..627
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            628..684
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            685..806
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            807..1014
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1161
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1266
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1396
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1459
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1524
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..3647
                     /gene="PFKFB4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3625..3630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFKFB4"
                     /note="hexamer: AATAAA"
     polyA_site      3647
                     /gene="PFKFB4"
                     /note="major polyA site"
ORIGIN      
        1 gcagtccgac tcatcccggc cccgggatgg cgtccccacg ggaattgaca cagaaccccc
       61 tgaagaagat ctggatgcca tacagcaatg ggcggcccgc tctgcacgct tgccagcgcg
      121 gtgaactttc aaaggtgggc tagttttcca acaggaggaa gggtgcctct tggccttaaa
      181 gctgagcttg ggcaggaact tcagcagaga tgtgctcagg atagagacag aaaagtatgc
      241 cgtgtgggcc tgtgtctgtg tcccgggaca ttgtgcatga ccaactgccc aactctcatt
      301 gtcatggtgg gcctgcccgc caggggcaag acctacatct ccaagaagct gactcgatac
      361 ctgaactgga ttggtgtgcc cactcgggag ttcaatgttg gccagtatcg ccgggacgtg
      421 gtcaagacct acaaatcttt tgaatttttt ctccccgaca atgaagaggg cctgaaaatc
      481 aggaagcagt gtgccctggc agccctccgt gacgtccggc ggttccttag tgaggagggg
      541 ggacatgtgg cggtttttga tgccacaaac accacccgag aacggagagc gaccatcttt
      601 aattttggag aacagaatgg ctacaagacc ttttttgtcg agtccatctg tgtggatcct
      661 gaggtcatag ctgccaacat cgtgcaagtg aaactgggca gccctgacta tgtcaaccgc
      721 gacagtgatg aggctacgga ggacttcatg aggcgcattg agtgctatga gaactcctac
      781 gagtcgctag atgaggacct ggatagggac ctgtcctata tcaagatcat ggatgtgggc
      841 cagagctacg tggtgaaccg tgtggctgac cacatccaga gccgcatcgt atattacctc
      901 atgaacatcc acgtgacccc ccgctccatc tacctctgcc ggcacgggga gagcgagctc
      961 aacctcaagg gccggattgg cggggaccca ggactgtccc ctcggggcag ggagtttgcc
     1021 aagagtctag cccagttcat cagtgaccaa aatatcaagg atctgaaggt ctggacaagc
     1081 cagatgaaga ggacaatcca gacggctgag gcactgggtg tgccctatga acagtggaag
     1141 gtcctcaacg agatcgatgc gggcgtctgt gaggaaatga cctacgagga aattcaggat
     1201 aattatccac tggagttcgc cctgcgggac caggacaagt accggtaccg gtaccctaaa
     1261 ggggagtcct acgaggacct ggtccagaga ctggagcctg tcatcatgga gctggagagg
     1321 caagagaatg tgctggtcat ctgccaccag gctgtgatgc gctgcctgct ggcctacttc
     1381 ctcgacaagg cagcagaaca gctgccctac ctcaagtgtc cgctgcacac agtcctgaag
     1441 ctgactcctg tggcatatgg ttgtaaagtg gagtccatat tcctgaacgt ggctgctgtg
     1501 aacacgcacc gggacaggcc tcagaacgtg gacatctcaa gacctccaga ggaagccctt
     1561 gtcacggtgc ctgctcacca gtgaccatgt tcatccactg tgaccactag gcaggcactg
     1621 ctctctgcag agggggtcat tccaggccct ccagtgtgtg tgatagtcac catgccatgc
     1681 agggatattc ttgaagccac acatggctgg cggaacccag agcccccacc ccagcccacc
     1741 tggctctttg ttgacagtcg gcgacaaggt tgtgcgtggc tcctgacctg ctgctaagag
     1801 tcacttgacc agactgcatc tgcatgggct gcgcggaggt tgcccagccc cagtttcttc
     1861 cggcgcagct cttaggtgtt cactctcgcc agctcagttg gctttgtgaa gtgtgaaacc
     1921 ctacaatgtg aaaggaaagt gcttgctgtg atgttcctac tgtggcccag ctgcccagca
     1981 tggacctggt gactctccac agggcctcta ccatcctctc tgtggccact tcctgagcca
     2041 gaggccaggt cttcatgggg ccctgagctt ctgctgcctc tggtgagagg gagagccctt
     2101 cccatcctta cccaccagga actagagccc caaccacagc agatgcttcc tgggcagcca
     2161 ctggccaggc cgttgtatcc atgtcaccct tagttgtggg cattcatgaa agcaatgcgc
     2221 ttgcttcagc acattgggat gcacagaaac gtgagggcag gggggcagtt gtggtcgccc
     2281 ctccccagtt gtgcatgtac ccggctactt aggtatgctt aggggagctg gtggccaggg
     2341 gtgccccttc aggcctgctc tcacagtgaa accacttcaa gacagggcag gaagttacct
     2401 tcctggtgca cacaccgaag tgctcgaggg agaggctgcc gccccctctc cccttccctg
     2461 gctgtggaca ctggcagcca atggggaggc ggcctctccc ctgttgctgc ccgtgtccac
     2521 acaggaatgt acactggtgg cagaaacatg catttgccat aaataattca gaaacacttg
     2581 tatggtcctg taaaacttct ctggtgtttg ttgacttctg cctgtgggtc tgaggaaggg
     2641 agaaggcaga acccataggc agtccctggg caagggagga ttttcccggc cgcagcacag
     2701 ccttccattc atgtgcacgt gagtctggtg aaaatggcct tgtgaagtcc agtgtctgtc
     2761 atgcccctga gttcttttgg tcccagagag gcagcctggg cagtctgttt acagctggca
     2821 aacagactgg ctggcaagtt gtggggctgg gtcagaaagt gtctggactt gtaatcgggt
     2881 gccctgctgt ccacaggtgg actctgggga agccaggccc ctggatccca agtcctttgt
     2941 gtccggagcc tttcctggcc tgtgcctgga gggtagcaca tctttccagc ctcccagggc
     3001 tttgcagcct gcagatgcca cctgtccctg agagaagtta gcgtccctgg ccctggccgg
     3061 tggtctctca tccttgtgtg ctgctctgct aagagatgtc caaggcggag ccggggcaag
     3121 atccttccag actcatctgt cagagcccca agccctttag acccagagcc caaggaccat
     3181 gcctttggga cattaggact gcagcctttg cttctgtgta ttttggagtt ttggtgactt
     3241 ttgtcacctg gacacactca tttgttagcc atagtgggtt cccttggtca gcaacagtgc
     3301 atgtacctct ggatgtcatc tgaggtgaga ccaccgaggc cttttctctc tgtgtacaga
     3361 ggggagttag gagttgctgg actggatgca ttacgaggac tggggacagg gtagagggac
     3421 atccagggat cagggcatga gtgggggcaa ccccccggcc tctgccctgg catggtctcc
     3481 gcatgggctg aggtgtagct gattggctgc cacatttcgg ccatgctggc tggcgtgccc
     3541 atgttgcaga tattttcccg agttccccag aatggatggt attgaatctc agccacatgc
     3601 aacactgtgt ccagcattct ttgcaataaa tactttttaa aaaataa
//
