LOCUS       NM_004566               4482 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
            (PFKFB3), transcript variant 1, mRNA.
ACCESSION   NM_004566
VERSION     NM_004566.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Lu,S., Zhao,R., Han,Y., Shao,S., Ji,Y., Zhang,J., Pan,H., Sun,J.
            and Feng,Y.
  TITLE     Identification of PFKFB3 as a key factor in the development of
            colorectal cancer and immunotherapy resistance
  JOURNAL   Clin Exp Med 24 (1), 219 (2024)
   PUBMED   39261380
  REMARK    GeneRIF: Identification of PFKFB3 as a key factor in the
            development of colorectal cancer and immunotherapy resistance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Jia,W., Wu,Q., Shen,M., Yu,X., An,S., Zhao,L., Huang,G. and Liu,J.
  TITLE     PFKFB3 regulates breast cancer tumorigenesis and Fulvestrant
            sensitivity by affecting ERalpha stability
  JOURNAL   Cell Signal 119, 111184 (2024)
   PUBMED   38640982
  REMARK    GeneRIF: PFKFB3 regulates breast cancer tumorigenesis and
            Fulvestrant sensitivity by affecting ERalpha stability.
REFERENCE   3  (bases 1 to 4482)
  AUTHORS   Chen,Y., Zhang,P., Han,F., Zhou,Y., Wei,J., Wang,C., Song,M.,
            Lin,S., Xu,Y. and Chen,X.
  TITLE     MiR-106a-5p targets PFKFB3 and improves sepsis through regulating
            macrophage pyroptosis and inflammatory response
  JOURNAL   J Biol Chem 300 (6), 107334 (2024)
   PUBMED   38705396
  REMARK    GeneRIF: MiR-106a-5p targets PFKFB3 and improves sepsis through
            regulating macrophage pyroptosis and inflammatory response.
REFERENCE   4  (bases 1 to 4482)
  AUTHORS   Cheng,Y., Wang,P. and Liu,L.
  TITLE     PFKFB3 Regulates the Growth and Migration of Ovarian Cancer Cells
            through Pyroptosis and Warburg Effect Progression
  JOURNAL   J Environ Pathol Toxicol Oncol 43 (4), 53-64 (2024)
   PUBMED   39016141
  REMARK    GeneRIF: PFKFB3 Regulates the Growth and Migration of Ovarian
            Cancer Cells through Pyroptosis and Warburg Effect Progression.
REFERENCE   5  (bases 1 to 4482)
  AUTHORS   Yalcin,A., Clem,B.F., Imbert-Fernandez,Y., Ozcan,S.C., Peker,S.,
            O'Neal,J., Klarer,A.C., Clem,A.L., Telang,S. and Chesney,J.
  TITLE     6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression
            and suppresses apoptosis via Cdk1-mediated phosphorylation of p27
  JOURNAL   Cell Death Dis 5 (7), e1337 (2014)
   PUBMED   25032860
  REMARK    GeneRIF: PFKFB3 promotes cell cycle progression and suppresses
            apoptosis via Cdk1-mediated phosphorylation of p27.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4482)
  AUTHORS   Mahlknecht,U., Chesney,J., Hoelzer,D. and Bucala,R.
  TITLE     Cloning and chromosomal characterization of the
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 gene
            (PFKFB3, iPFK2)
  JOURNAL   Int J Oncol 23 (4), 883-891 (2003)
   PUBMED   12963966
  REMARK    GeneRIF: PFKFB3 cloning and characterization
REFERENCE   7  (bases 1 to 4482)
  AUTHORS   Manzano,A., Rosa,J.L., Ventura,F., Perez,J.X., Nadal,M.,
            Estivill,X., Ambrosio,S., Gil,J. and Bartrons,R.
  TITLE     Molecular cloning, expression, and chromosomal localization of a
            ubiquitously expressed human 6-phosphofructo-2-kinase/ fructose-2,
            6-bisphosphatase gene (PFKFB3)
  JOURNAL   Cytogenet Cell Genet 83 (3-4), 214-217 (1998)
   PUBMED   10072580
REFERENCE   8  (bases 1 to 4482)
  AUTHORS   Sakai,A., Kato,M., Fukasawa,M., Ishiguro,M., Furuya,E. and
            Sakakibara,R.
  TITLE     Cloning of cDNA encoding for a novel isozyme of fructose
            6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human
            placenta
  JOURNAL   J Biochem 119 (3), 506-511 (1996)
   PUBMED   8830046
REFERENCE   9  (bases 1 to 4482)
  AUTHORS   Pilkis,S.J., Claus,T.H., Kurland,I.J. and Lange,A.J.
  TITLE     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic
            signaling enzyme
  JOURNAL   Annu Rev Biochem 64, 799-835 (1995)
   PUBMED   7574501
  REMARK    Review article
REFERENCE   10 (bases 1 to 4482)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB068734.1, AL157395.17,
            AK291263.1, AL359960.23 and AI633567.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004566.3.
            
            Summary: The protein encoded by this gene belongs to a family of
            bifunctional proteins that are involved in both the synthesis and
            degradation of fructose-2,6-bisphosphate, a regulatory molecule
            that controls glycolysis in eukaryotes. The encoded protein has a
            6-phosphofructo-2-kinase activity that catalyzes the synthesis of
            fructose-2,6-bisphosphate (F2,6BP), and a
            fructose-2,6-biphosphatase activity that catalyzes the degradation
            of F2,6BP. This protein is required for cell cycle progression and
            prevention of apoptosis. It functions as a regulator of
            cyclin-dependent kinase 1, linking glucose metabolism to cell
            proliferation and survival in tumor cells. Several alternatively
            spliced transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040482.1, SRR1803616.54660.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379775.9/ ENSP00000369100.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                DB068734.1         56-79
            25-25               AL157395.17        147051-147051
            26-2011             AK291263.1         26-2011
            2012-4220           AL359960.23        4318-6526
            4221-4482           AI633567.1         17-278              c
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p15.1"
     gene            1..4482
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     3"
                     /db_xref="GeneID:5209"
                     /db_xref="HGNC:HGNC:8874"
                     /db_xref="MIM:605319"
     exon            1..405
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             330..1892
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform 1 is encoded by transcript variant 1;
                     6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase;
                     inducible
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase;
                     fructose-6-phosphate,2-kinase/fructose-2,
                     6-bisphosphatase; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3;
                     renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase
                     brain/placenta-type isozyme;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 3 isoform 1"
                     /protein_id="NP_004557.1"
                     /db_xref="CCDS:CCDS7078.1"
                     /db_xref="GeneID:5209"
                     /db_xref="HGNC:HGNC:8874"
                     /db_xref="MIM:605319"
                     /translation="MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLP
                     ARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQC
                     ALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTV
                     VASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDV
                     GRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGK
                     KFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTY
                     EEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVL
                     RCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAK
                     KGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMK
                     GSRSSADSSRKH"
     exon            406..531
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            532..628
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            629..695
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            696..770
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            771..827
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            828..952
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1160
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1307
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1412
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1542
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1605
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1670
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1844
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..4482
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4459..4464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /note="hexamer: AATAAA"
     polyA_site      4482
                     /gene="PFKFB3"
                     /gene_synonym="iPFK-2; IPFK2; PFK2"
                     /note="major polyA site"
ORIGIN      
        1 agccaagccg gagaggaggc gagcagcagg gcctggtggc gagagcgcgg ctgtcactgc
       61 gcccgagcat cccagagctt tccgagcgga cgagccggcc gtgccgggca tccccagcct
      121 cgctaccctc gcagcacacg tcgagccccg cacaggcgag ggtccggaac ttagcccaaa
      181 gcacgtttcc cctggcagcg caggaaacgc ccggccgcgc gccggcgcac gcccccctct
      241 cctcctttgt tccgggggtc ggcggccgct ctcctgccag cgtcgggatc tcggccccgg
      301 gaggcgggcc gtcgggcgca gccgcgaaga tgccgttgga actgacgcag agccgagtgc
      361 agaagatctg ggtgcccgtg gaccacaggc cctcgttgcc cagatcctgt gggccaaagc
      421 tgaccaactc ccccaccgtc atcgtcatgg tgggcctccc cgcccggggc aagacctaca
      481 tctccaagaa gctgactcgc tacctcaact ggattggcgt ccccacaaaa gtgttcaacg
      541 tcggggagta tcgccgggag gctgtgaagc agtacagctc ctacaacttc ttccgccccg
      601 acaatgagga agccatgaaa gtccggaagc aatgtgcctt agctgccttg agagatgtca
      661 aaagctacct ggcgaaagaa gggggacaaa ttgcggtttt cgatgccacc aatactacta
      721 gagagaggag acacatgatc cttcattttg ccaaagaaaa tgactttaag gcgtttttca
      781 tcgagtcggt gtgcgacgac cctacagttg tggcctccaa tatcatggaa gttaaaatct
      841 ccagcccgga ttacaaagac tgcaactcgg cagaagccat ggacgacttc atgaagagga
      901 tcagttgcta tgaagccagc taccagcccc tcgaccccga caaatgcgac agggacttgt
      961 cgctgatcaa ggtgattgac gtgggccgga ggttcctggt gaaccgggtg caggaccaca
     1021 tccagagccg catcgtgtac tacctgatga acatccacgt gcagccgcgt accatctacc
     1081 tgtgccggca cggcgagaac gagcacaacc tccagggccg catcgggggc gactcaggcc
     1141 tgtccagccg gggcaagaag tttgccagtg ctctgagcaa gttcgtggag gagcagaacc
     1201 tgaaggacct gcgcgtgtgg accagccagc tgaagagcac catccagacg gccgaggcgc
     1261 tgcggctgcc ctacgagcag tggaaggcgc tcaatgagat cgacgcgggc gtctgtgagg
     1321 agctgaccta cgaggagatc agggacacct accctgagga gtatgcgctg cgggagcagg
     1381 acaagtacta ttaccgctac cccaccgggg agtcctacca ggacctggtc cagcgcttgg
     1441 agccagtgat catggagctg gagcggcagg agaatgtgct ggtcatctgc caccaggccg
     1501 tcctgcgctg cctgcttgcc tacttcctgg ataagagtgc agaggagatg ccctacctga
     1561 aatgccctct tcacaccgtc ctgaaactga cgcctgtcgc ttatggctgc cgtgtggaat
     1621 ccatctacct gaacgtggag tccgtctgca cacaccggga gaggtcagag gatgcaaaga
     1681 agggacctaa cccgctcatg agacgcaata gtgtcacccc gctagccagc cccgaaccca
     1741 ccaaaaagcc tcgcatcaac agctttgagg agcatgtggc ctccacctcg gccgccctgc
     1801 ccagctgcct gcccccggag gtgcccacgc agctgcctgg acaaaacatg aaaggctccc
     1861 ggagcagcgc tgactcctcc aggaaacact gaggcagacg tgtcggttcc attccatttc
     1921 catttctgca gcttagcttg tgtcctgccc tccgcccgag gcaaaacgta tcctgaggac
     1981 ttcttccgga gagggtgggg tggagcagcg ggggagcctt ggccgaagag aaccatgctt
     2041 ggcaccgtct gtgtcccctc ggccgctgga caccagaaag ccacgtgggt ccctggcgcc
     2101 ctgcctttag ccgtggggcc cccacctcca ctctctgggt ttcctaggaa tgtccagcct
     2161 cggagacctt cacaaagcct tgggagggtg atgagtgctg gtcctgacag gaggccgctg
     2221 gggacactgt gctgttttgt ttcgtttctg tgatctcccg gcacgtttgg agctgggaag
     2281 accacactgg tggcagaatc ctaaaattaa aggaggcagg ctcctagttg ctgaaagtta
     2341 aggaatgtgt aaaacctcca cgtgactgtt tggtgcatct tgacctggga agacgcctca
     2401 tgggaacgaa cttggacagg tgttgggttg aggcctcttc tgcaggaagt ccctgagctg
     2461 agacgcaagt tggctgggtg gtccgcaccc tggctctcct gcaggtccac acaccttcca
     2521 ggcctgtggc ctgcctccaa agatgtgcaa gggcaggctg gctgcacggg gagagggaag
     2581 tattttgccg aaatatgaga actggggcct cctgctccca gggagctcca gggcccctct
     2641 ctcctcccac ctggacttgg ggggaactga gaaacacttt cctggagctg ctggcttttg
     2701 cacttttttg atggcagaag tgtgacctga gagtcccacc ttctcttcag gaacgtagat
     2761 gttggggtgt cttgccctgg ggggcttgga acctctgaag gtggggagcg gaacacctgg
     2821 catccttccc cagcacttgc attaccgtcc ctgctcttcc caggtgggga cagtggccca
     2881 agcaaggcct cactcgcagc cacttcttca agagctgcct gcacactgtc ttggagcatc
     2941 tgccttgtgc ctggcactct gccggtgcct tgggaaggtc ggaagagtgg actttgtcct
     3001 ggccttccct tcatggcgtc tatgacactt ttgtggtgat ggaaagcatg ggacctgtcg
     3061 tctcagcctg ttggtttctc ctcattgcct caaaccctgg ggtaggtggg acggggggtc
     3121 tcgtgcccag atgaaaccat ttggaaactc ggcagcagag tttgtccaaa tgaccctttt
     3181 caggatgtct caaagcttgt gccaaaggtc acttttcttt cctgccttct gctgtgagcc
     3241 ctgagatcct cctcccagct caagggacag gtcctgggtg agggtgggag atttagacac
     3301 ctgaaactgg gcgtggagag aagagccgtt gctgtttgtt ttttgggaag agcttttaaa
     3361 gaatgcatgt ttttttcctg gttggaattg agtaggaact gaggctgtgc ttcaggtatg
     3421 gtacaatcaa gtgggggatt ttcatgctga accattcaag ccctccccgc ccgttgcacc
     3481 cactttggct ggcgtctgct ggagaggatg tctctgtccg cattcccgtg cagctccagg
     3541 ctcgcgcagt tttctctctc tccctggatg ttgagtctca tcagaatatg tgggtagggg
     3601 gtggacgtgc acgggtgcat gattgtgctt aacttggttg tatttttcga tttgacatgg
     3661 aaggcctgtt gctttgctct tgagaatagt ttctcgtgtc cccctcgcag gcctcattct
     3721 ttgaacatcg actctgaagt ttgatacaga taggggcttg atagctgtgg tcccctctcc
     3781 cctctgacta cctaaaatca atacctaaat acagaagcct tggtctaaca cgggactttt
     3841 agtttgcgaa gggcctagat agggagagag gtaacatgaa tctggacagg gagggagata
     3901 ctatagaaag gagaacactg cctactttgc aagccagtga cctgcctttt gaggggacat
     3961 tggacggggg ccgggggcgg gggttgggtt tgagctacag tcatgaactt ttggcgtcta
     4021 ctgattcctc caactctcca ccccacaaaa taacggggac caatattttt aactttgcct
     4081 atttgttttt gggtgagttt cccccctcct tattctgtcc tgagaccacg ggcaaagctc
     4141 ttcattttga gagagaagaa aaactgtttg gaaccacacc aatgatattt ttctttgtaa
     4201 tacttgaaat ttattttttt attattttga tagcagatgt gctatttatt tatttaatat
     4261 gtataaggag cctaaacaat agaaagctgt agagattggg tttcattgtt aattggtttg
     4321 ggagcctcct atgtgtgact tatgacttct ctgtgttctg tgtatttgtc tgaattaatg
     4381 acctgggata taaagctatg ctagctttca aacaggagat gcctttcaga aatttgtata
     4441 ttttgcagtt gccagaccaa taaaatacct ggttgaaata ca
//
