LOCUS       NM_006212               7073 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
            (PFKFB2), transcript variant 1, mRNA.
ACCESSION   NM_006212
VERSION     NM_006212.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Zhang,L. and Liu,J.
  TITLE     miR-21-5p inhibits the growth of brain glioma cells through
            regulating the glycolysis mediated by PFKFB2
  JOURNAL   Funct Integr Genomics 23 (4), 322 (2023)
   PUBMED   37864733
  REMARK    GeneRIF: miR-21-5p inhibits the growth of brain glioma cells
            through regulating the glycolysis mediated by PFKFB2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Liu,F., Wei,X., Chen,Z., Chen,Y., Hu,P. and Jin,Y.
  TITLE     PFKFB2 is a favorable prognostic biomarker for colorectal cancer by
            suppressing metastasis and tumor glycolysis
  JOURNAL   J Cancer Res Clin Oncol 149 (12), 10737-10752 (2023)
   PUBMED   37311985
  REMARK    GeneRIF: PFKFB2 is a favorable prognostic biomarker for colorectal
            cancer by suppressing metastasis and tumor glycolysis.
REFERENCE   3  (bases 1 to 7073)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 7073)
  AUTHORS   Shen,X., Zhu,X., Hu,P., Ji,T., Qin,Y. and Zhu,J.
  TITLE     Knockdown circZNF131 Inhibits Cell Progression and Glycolysis in
            Gastric Cancer Through miR-186-5p/PFKFB2 Axis
  JOURNAL   Biochem Genet 60 (5), 1567-1584 (2022)
   PUBMED   35059934
  REMARK    GeneRIF: Knockdown circZNF131 Inhibits Cell Progression and
            Glycolysis in Gastric Cancer Through miR-186-5p/PFKFB2 Axis.
REFERENCE   5  (bases 1 to 7073)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 7073)
  AUTHORS   Pilkis,S.J., Claus,T.H., Kurland,I.J. and Lange,A.J.
  TITLE     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic
            signaling enzyme
  JOURNAL   Annu Rev Biochem 64, 799-835 (1995)
   PUBMED   7574501
  REMARK    Review article
REFERENCE   7  (bases 1 to 7073)
  AUTHORS   Goldmuntz,E.A., Remmers,E.F., Zha,H., Mathern,P., Du,Y.,
            Crofford,L.J. and Wilder,R.L.
  TITLE     Genetic map of seven polymorphic markers comprising a single
            linkage group on rat chromosome 5
  JOURNAL   Mamm Genome 4 (11), 670-675 (1993)
   PUBMED   7904197
REFERENCE   8  (bases 1 to 7073)
  AUTHORS   Rider,M.H., Vandamme,J., Lebeau,E., Vertommen,D., Vidal,H.,
            Rousseau,G.G., Vandekerckhove,J. and Hue,L.
  TITLE     The two forms of bovine heart
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from
            alternative splicing
  JOURNAL   Biochem J 285 (Pt 2) (Pt 2), 405-411 (1992)
   PUBMED   1322130
REFERENCE   9  (bases 1 to 7073)
  AUTHORS   Hilliker,C.E., Darville,M.I., Aly,M.S., Chikri,M., Szpirer,C.,
            Marynen,P., Rousseau,G.G. and Cassiman,J.J.
  TITLE     Human and rat chromosomal localization of two genes for
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of
            somatic cell hybrids and in situ hybridization
  JOURNAL   Genomics 10 (4), 867-873 (1991)
   PUBMED   1655632
REFERENCE   10 (bases 1 to 7073)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX510096.1, BC069583.1 and
            AL445493.8.
            
            On May 13, 2005 this sequence version replaced NM_006212.1.
            
            Summary: The protein encoded by this gene is involved in both the
            synthesis and degradation of fructose-2,6-bisphosphate, a
            regulatory molecule that controls glycolysis in eukaryotes. The
            encoded protein has a 6-phosphofructo-2-kinase activity that
            catalyzes the synthesis of fructose-2,6-bisphosphate, and a
            fructose-2,6-biphosphatase activity that catalyzes the degradation
            of fructose-2,6-bisphosphate. This protein regulates
            fructose-2,6-bisphosphate levels in the heart, while a related
            enzyme encoded by a different gene regulates
            fructose-2,6-bisphosphate levels in the liver and muscle. This
            enzyme functions as a homodimer. Two transcript variants encoding
            two different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.255565.1,
                                           SRR1803615.19660.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367080.8/ ENSP00000356047.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               BX510096.1         2-195
            195-1699            BC069583.1         136-1640
            1700-7073           AL445493.8         31914-37287
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..7073
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     2"
                     /db_xref="GeneID:5208"
                     /db_xref="HGNC:HGNC:8873"
                     /db_xref="MIM:171835"
     exon            1..92
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            93..194
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1627
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform a is encoded by transcript variant 1;
                     PFKFB, cardiac; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme;
                     fructose-2,6-bisphosphatase, cardiac isozyme;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2;
                     PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase 2"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 2 isoform a"
                     /protein_id="NP_006203.2"
                     /db_xref="CCDS:CCDS31004.1"
                     /db_xref="GeneID:5208"
                     /db_xref="HGNC:HGNC:8873"
                     /db_xref="MIM:171835"
                     /translation="MSGASSSEQNNNSYETKTPNLRMSEKKCSWASYMTNSPTLIVMI
                     GLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIR
                     KQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMILNFAEQNSFKVFFVESVCDD
                     PDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYRPLDPDNYDKDLSFIKV
                     INVGQRFLVNRVQDYIQSKIVYYLMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSV
                     RGKQFAQALRKFLEEQEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEE
                     MTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQ
                     AVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVNTHRDKPT
                     NNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRAQDMQEGAD"
     exon            195..320
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            321..417
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            418..484
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..559
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            560..616
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            617..741
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            742..949
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1096
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1201
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1331
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1394
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1459
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..7073
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6171..6176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /note="hexamer: ATTAAA"
     polyA_site      6197
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /note="major polyA site"
     regulatory      7046..7051
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /note="hexamer: AATAAA"
     polyA_site      7073
                     /gene="PFKFB2"
                     /gene_synonym="PFK-2/FBPase-2"
                     /note="major polyA site"
ORIGIN      
        1 ggtcccggcc acaagctgtc ggctcggttc ggtcgcgtta cagggcaggc gccggggcca
       61 aggcagggag ggatcttagg agagatcgta ggacatctga agagctgcca tgtctggggc
      121 atcttcctca gaacagaaca acaacagcta tgaaaccaaa accccaaatc ttcgaatgtc
      181 agagaagaaa tgttcatggg cctcctacat gaccaactcc ccgactctga tcgttatgat
      241 tggtttgcca gcccggggta aaacctacgt gtccaagaaa ctaacacgct acctcaactg
      301 gattggagtc cccaccaaag tgtttaatct tggggtgtat cggcgtgaag cagtcaagtc
      361 ctataagtcc tacgacttct ttcggcatga caatgaggag gccatgaaga tccgcaaaca
      421 gtgtgctctg gtggcgctgg aagatgttaa ggcgtatctc actgaggaga atggtcagat
      481 tgcggtgttt gatgccacca atacaacccg ggagaggagg gacatgattt tgaactttgc
      541 tgaacagaat tccttcaagg tattctttgt ggaatccgtc tgtgatgatc ctgatgtcat
      601 tgctgccaat attctggagg ttaaggtatc aagccctgac tatcctgaaa ggaacagaga
      661 gaacgtgatg gaggacttcc tgaagagaat tgaatgctac aaagttacct accgacctct
      721 tgacccagac aactatgaca aggatctttc tttcatcaag gtgataaacg tgggccagcg
      781 atttttagtc aacagagtcc aggactacat ccagagcaag atagtctact acctcatgaa
      841 tatccacgtc cagcctcgca ccatttacct ttgccggcat ggagaaagcg agttcaatct
      901 cttggggaag attgggggtg actctggcct ctcggtgcgg ggaaagcagt ttgcccaagc
      961 tctaaggaaa tttctggagg aacaggaaat aacagacctc aaagtgtgga caagccagtt
     1021 gaagaggacc atacagactg ctgaatctct cggggtgccc tatgagcagt ggaagattct
     1081 gaatgagatt gatgctggtg tgtgtgaaga gatgacctat gcagagattg agaaacggta
     1141 cccagaagag tttgcacttc gagatcaaga gaagtatctg tatcgatatc ctggtgggga
     1201 gtcataccag gacctggtgc agcggctgga gcctgtcatc atggagctgg aacgtcaggg
     1261 caatgtcctc gtcatctccc accaggctgt catgcgctgc ctcctggcct acttcttgga
     1321 taagggcgca gatgagctac catacttgag atgccctctc cataccatct tcaaacttac
     1381 tcctgtggcc tatgggtgca aagtggaaac aattaaactt aacgtggagg ctgtgaacac
     1441 gcaccgtgac aagccaacta acaacttccc caagaaccaa acccctgtaa ggatgagaag
     1501 gaacagcttt acgcctctgt ccagttcgaa tacaataagg cgtccaagaa attacagtgt
     1561 tgggagccgg cccctcaagc ccctcagccc tctccgtgcc caggacatgc aagaaggggc
     1621 cgactagccg aagacccaag tcagcattcc ggtggtgtaa ctgtgtgttt ccctccagcc
     1681 ctggcctcct gcccttgtca ctaatcacca aggagtcatt aacttcctcc ctctatgccc
     1741 acccctgaca cttcaccatt aatcttaaca cagaacatga ggttatgtgt ttataggaca
     1801 acttaagctg ttcttcagtt tgaaacatct tttcacccag gggcaagtta gcaaattgag
     1861 tcttttagga cagattctca gatgggatga tctggaggag gaggaaaaag atacactccc
     1921 ttggggctga gcatgcccca cactcttgga tcttctctct gttccctggt gtcttcacta
     1981 atgtcctcat gttggtgaag tgttggggga tggagggcgg gtgagcagtc gggggacaaa
     2041 aagtctattt ttccttcact tttgtctctt caatgtgtgt tttcctcaca ctccttactt
     2101 gactgctttc ttcccccact ttcatgtccc ctctggattg tccagtgtaa tgcatggcat
     2161 tgtggtgtct gtctaggaag gaaggggtga ttgacaagag acaagtctgg cccagttaat
     2221 gctttctgca gtgggtctaa atggatctgg actggaggag tctttccttt cctttctccc
     2281 ttcctctcca gccaggtcct gtaagggcca gcccagacta caggacatcc acagaatatt
     2341 ctggagcttg caagtagaca tagggtgaga gttcttgcct cctttcatga gaaacagttc
     2401 taagtcttcg atgccctggg agaatctggc tctgagcatg tgggaaatgt cttggttttt
     2461 atttttaatt tagagtcagg cctgggtctt ttgaggtctg cagagtgggg tttaggagag
     2521 tagggtgggc tctagctctt gcagggctct tagagagcag tcatgtcttt tctcccatga
     2581 ctctcaggtt ctttgccaat cacagcaact tttcctgcca aagccagtat cctctggggc
     2641 tgtttagaag ggcagttaga ttcaggagtc accactgatg tttgagttgc tcaaggcaag
     2701 aggcagagaa gagttcacta aaactgctta tttttgaata atttcagcac actgtcctta
     2761 agaagaaaga aacatcaaaa caaaatagtt tttacatgac catttttttc ccaaatgtgg
     2821 aaaagcttga tgatgaattt aatctctctc attggagtat tcttttgttc ataaagagaa
     2881 actatctcat cttgatgtcc agagaagtcc ttggaaccct gtgggatcta gctcgtaact
     2941 gtttgtattt ctctattcac ttctgtcatt tcattttctt tgtagggtta aacagaaaat
     3001 gtttagggaa gaaattctta gccccttgat gaccatgatg gcttattctc tttcccaatt
     3061 ttgcatgcaa aatgtacgaa tatatgtatg tttttctgag aggcaagttt agggttctca
     3121 tgggatttta aaaagagata ggtgactccc caccttaaag ttatctgctg gtcttttaga
     3181 ggtaaccagt gagaaggagt tagctatttt ccaagggtgt tttcatttgg taatggaaga
     3241 ctttttgctg tggttctcat ccaggagtat tccttagaat tgcctttagg attgttgaat
     3301 cataaacatg cctgtgtcca ccttatgctt aatactgact cagaatctct gggatctggg
     3361 tctggacatg catgtattta agtagcttcc cggatgattc tgattcatag ctcgggttaa
     3421 gaactccacc ttaggaagtt aggtggaaaa atactggata gtagtttctc agacaaaagt
     3481 gtcagcctag gaattctgaa ttcctcatag tcctgaaagg acagttcttt gggtttgatg
     3541 tttgagcaga tcataatgtt aaatgatata accccctgga aggcaggctg ctgtgtttta
     3601 gagggttatt ctaggttcta gtcccatctt taacccttta cattgctgag tgatgtggaa
     3661 caaatcactg aattaaataa ttgtctctag agagcagctg gggattttaa gcaccctgga
     3721 gagaggtgca gaccctgcca ggatgataaa ggttgtcatc actggcaact gactcctgac
     3781 cccgggtcct ttactcgtat gtcccaagta ggataccata ggcagcttct aaaggctgct
     3841 tacctagatt cttctcaaaa ttgtgtccaa gatgttaagt aacctgctat tcctgtttag
     3901 tggttacata acatgtactt agtgtctttg tggagctttg ggctggtagg ggcagggata
     3961 ggggaaagct aaacagaagt agaagaaaag gtccttgtcc agaggaaggt tattttaaga
     4021 gacaggacat gtaatttata acaaatggac atttgcaata tagcaacagt ctgagtctag
     4081 aagtgttcaa ccatcacatc gcttctcctg acttttctgt tgtcctatgg ctatgagact
     4141 acaggggttg ggggatgggg atgcccccac caccaccagt gtgagaatga gatatggttg
     4201 gggaggcgtg tttggcactt ttacaaggtt gcattgtcca catttgcttg gatggtggca
     4261 tctgtagccc aaatgggcat tcagtctttt cttctgctgt gaggtaagag taattcagac
     4321 ctaatgaatg agaagaggga gcactttgat ccacagactt tggattaaca ctgtacccac
     4381 tcttagcaaa aggggacttc tctaacaagc tagcattgtg ttaacctgtc attaacccag
     4441 cattagagac aggtcactgg gatgttcatt ctggctctca gcctgctgtt atttccccac
     4501 tctcacctgt gctagttcgc tttctccaga agaggagctg aggtggtctt atcctcagat
     4561 aggacatgag aaattggaat ttggcaagca agggcttcag catcctttag tttctaaagc
     4621 aagatccctt cagagaagtc tagcaggaag aagccaggag aatgtagaat ggaaaagagc
     4681 tcttactcca aattttagag aagtttcagc tctacttctc atcttttctc tcctggtctt
     4741 acttgaatgc atgttggtaa tctgtataca ttactatgac tgtgtctatc acaagtccta
     4801 caaaataaaa atggacttaa aagtactctc tgctgaggct gtaagttttg ttttgtgaga
     4861 aataggtaaa gacattagca tttaatctac tggaataagc tggttgctgt ggcttatacc
     4921 tgtagtccca gatacttggg aggctgaggc aggagaatca cttgagacca gcctgggcaa
     4981 tatagtgaga cctcacctct aaaaaagttt tttataaatt tacttgtcta aagtggggaa
     5041 agggaaatta ttctgttttc ttattcttgc ttcaagacta tgacagattt gaaagagaat
     5101 tctaaagcag atttagagaa cctgcttctc ttcttatctc ctaatcccta aattgtaatt
     5161 tagctcttat ctgtattgtt gttttgtttt ggtaaaggga tgatttttac attgagtttt
     5221 aaagtagaat aagaaaagct tctaaaaact tgcattgtgc tagggatctg ccctatatct
     5281 ttgcctctgg tgtttcgttg ttgttgttat tgtttgtttg tttccaaaga agttggagtt
     5341 aaggacacaa tatatttgta cccctagact gaatgggtga gtattccata tgaggatctg
     5401 ggtaatcctc tttgcaaccc acatttggtc ttcagagaca ctggcatttt gaagaaacat
     5461 atgatatagc tgtttggaaa taaattcatc tatgttactt tttttttctt tttttttttt
     5521 ttttatgagc aggagatctt aattgacaga aactcattgg tggttggagt ggccaatggg
     5581 cacgggaaaa agtatccagt aatcagaaga attgtatctg ggttatgtaa tcttatgcac
     5641 attccattgt ctttgccaag cccagaagcc atgttgtgtt cattgttaag aaatttgata
     5701 gatttaccca gcttttctat gtattttgac ttattgaaaa tatgtaacaa ctgagtcggg
     5761 ttgcagcact ggtggggtag aatcgacttt ccctgaaggt gacacagatg tcagaattgt
     5821 gtccagggat ttaatttaga cccatactgt ccaggagact gtctctagtt ggatctctgt
     5881 gctgactgac tgacagacag actttagtgt ctgtgtgctg actgacagac tctagtagtg
     5941 tctatatgtt gaccaactgg tagaccagga ggatctgtgt gctgattgac tctagtagga
     6001 tctgtttgtc actgacagac tgtagtagtg tctgtgtgct gactgataga tagactatag
     6061 taaaatttgg gtgttgcctg actaacggtc tagggtctgt aagctgacag tctgcctgct
     6121 ttctgattgt atccattgaa gtgtatgtac attatggtaa ttctctgtct attaaatgtg
     6181 tctaacaaag gaaggaatta agcactccac gtgttttctt tataggggag ttctgtacac
     6241 tatgatttta aatagatatt tcttatatag tagtggccaa attctcatta ttttgtacaa
     6301 gataaaggtt atgcatcact tttatggtat tttgtgaact cagctaaggg aatgcctgtt
     6361 cagagcctgg agttgttacc tttacttgaa gtcatctcat ccagtcccct gctttagggc
     6421 aggacttcag ttccactgtt catttctgaa gcttctgtgt ccccagctta ccctgttctg
     6481 aaatgttgta ttccattgga cagggctgct atttttagtc agccatgcat ttggatttta
     6541 cacttaatct agtaagtaaa aatgagaaga aaatttggca tttaaaaatt gattttaagg
     6601 gttggcaaaa gtattttttc cagtaagcct ttcactggat atctgtgacc aatgtttacc
     6661 tacgcaatgt ttttgtatct gaattgctta tgtacgtttt ttattatatt gacctaacaa
     6721 gaagatcaac ttatgctggt atggtgatgg ttttgctatg gcaaaatcaa agggctgatc
     6781 atacatggtg ccctttggga agggggatgg tgtggggctg agcacctctg ggttgaatgg
     6841 gaatgggtca gattgggaag cctaggaaga gagttctact gtagatttcc taggcactgc
     6901 tctgttgaaa taggaacata agtctttagc aacattctga tttaatcggg tgacactgat
     6961 aacaaagtat gccactcaga tccatttaaa gtgtgcataa ctgtatttga aatgtgtttt
     7021 tgtgtgcgtg tgtgtagaat gggtaaataa aattgttgag taacttgaac cta
//
