LOCUS       NM_001185181             734 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens prefoldin subunit 6 (PFDN6), transcript variant 1,
            mRNA.
ACCESSION   NM_001185181
VERSION     NM_001185181.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 734)
  AUTHORS   Liang,J., Xia,L., Oyang,L., Lin,J., Tan,S., Yi,P., Han,Y., Luo,X.,
            Wang,H., Tang,L., Pan,Q., Tian,Y., Rao,S., Su,M., Shi,Y., Cao,D.,
            Zhou,Y. and Liao,Q.
  TITLE     The functions and mechanisms of prefoldin complex and
            prefoldin-subunits
  JOURNAL   Cell Biosci 10, 87 (2020)
   PUBMED   32699605
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 734)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 734)
  AUTHORS   Cloutier,P., Poitras,C., Faubert,D., Bouchard,A., Blanchette,M.,
            Gauthier,M.S. and Coulombe,B.
  TITLE     Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of
            the PAQosome
  JOURNAL   J Proteome Res 19 (1), 18-27 (2020)
   PUBMED   31738558
REFERENCE   5  (bases 1 to 734)
  AUTHORS   Gestaut,D., Roh,S.H., Ma,B., Pintilie,G., Joachimiak,L.A.,
            Leitner,A., Walzthoeni,T., Aebersold,R., Chiu,W. and Frydman,J.
  TITLE     The Chaperonin TRiC/CCT Associates with Prefoldin through a
            Conserved Electrostatic Interface Essential for Cellular
            Proteostasis
  JOURNAL   Cell 177 (3), 751-765 (2019)
   PUBMED   30955883
REFERENCE   6  (bases 1 to 734)
  AUTHORS   Simons,C.T., Staes,A., Rommelaere,H., Ampe,C., Lewis,S.A. and
            Cowan,N.J.
  TITLE     Selective contribution of eukaryotic prefoldin subunits to actin
            and tubulin binding
  JOURNAL   J Biol Chem 279 (6), 4196-4203 (2004)
   PUBMED   14634002
REFERENCE   7  (bases 1 to 734)
  AUTHORS   Hansen,W.J., Cowan,N.J. and Welch,W.J.
  TITLE     Prefoldin-nascent chain complexes in the folding of cytoskeletal
            proteins
  JOURNAL   J Cell Biol 145 (2), 265-277 (1999)
   PUBMED   10209023
REFERENCE   8  (bases 1 to 734)
  AUTHORS   Vainberg,I.E., Lewis,S.A., Rommelaere,H., Ampe,C.,
            Vandekerckhove,J., Klein,H.L. and Cowan,N.J.
  TITLE     Prefoldin, a chaperone that delivers unfolded proteins to cytosolic
            chaperonin
  JOURNAL   Cell 93 (5), 863-873 (1998)
   PUBMED   9630229
REFERENCE   9  (bases 1 to 734)
  AUTHORS   Herberg,J.A., Beck,S. and Trowsdale,J.
  TITLE     TAPASIN, DAXX, RGL2, HKE2 and four new genes (BING 1, 3 to 5) form
            a dense cluster at the centromeric end of the MHC
  JOURNAL   J Mol Biol 277 (4), 839-857 (1998)
   PUBMED   9545376
REFERENCE   10 (bases 1 to 734)
  AUTHORS   Herberg,J.A., Sgouros,J., Jones,T., Copeman,J., Humphray,S.J.,
            Sheer,D., Cresswell,P., Beck,S. and Trowsdale,J.
  TITLE     Genomic analysis of the Tapasin gene, located close to the TAP loci
            in the MHC
  JOURNAL   Eur J Immunol 28 (2), 459-467 (1998)
   PUBMED   9521053
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL662820.6, CB124036.1, BM686351.1 and BE794658.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001185181.2.
            
            Summary: PFDN6 is a subunit of the heteromeric prefoldin complex
            that chaperones nascent actin (see MIM 102560) and alpha- and
            beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains
            pending their transfer to the cytosolic chaperonin containing TCP1
            (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed
            10209023]).[supplied by OMIM, Jul 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. All variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI914776.1, SRR1163658.494215.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374606.10/ ENSP00000363734.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AL662820.6         18605-18638
            35-60               CB124036.1         1-26
            61-619              BM686351.1         8-566
            620-734             BE794658.1         439-553
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.32"
     gene            1..734
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /note="prefoldin subunit 6"
                     /db_xref="GeneID:10471"
                     /db_xref="HGNC:HGNC:4926"
                     /db_xref="MIM:605660"
     exon            1..320
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..646
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /note="KE2 protein; HLA class II region expressed gene
                     KE2"
                     /codon_start=1
                     /product="prefoldin subunit 6"
                     /protein_id="NP_001172110.1"
                     /db_xref="CCDS:CCDS4773.1"
                     /db_xref="GeneID:10471"
                     /db_xref="HGNC:HGNC:4926"
                     /db_xref="MIM:605660"
                     /translation="MAELIQKKLQGEVEKYQQLQKDLSKSMSGRQKLEAQLTENNIVK
                     EELALLDGSNVVFKLLGPVLVKQELGEARATVGKRLDYITAEIKRYESQLRDLERQSE
                     QQRETLAQLQQEFQRAQAAKAGAPGKA"
     exon            321..391
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /inference="alignment:Splign:2.1.0"
     exon            392..516
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /inference="alignment:Splign:2.1.0"
     exon            517..734
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      713..718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /note="hexamer: AATAAA"
     polyA_site      734
                     /gene="PFDN6"
                     /gene_synonym="H2-KE2; HKE2; KE-2; PFD6"
                     /note="major polyA site"
ORIGIN      
        1 attactactg aaggaagaac gtgagtaggt taggatttcg gttgagaggc ttggggtctt
       61 gcgtttcgcc caccatctcc tggggacagg gtggagtcga tatccgggac gggggggagg
      121 ttgcggtgcc cctcagggct acctctcaag agtgctatca tttccgcagg ccagatcaga
      181 aaagggagct caggtacctt ccagagagtg agacccagcg cccttgtctc gcacccagta
      241 ggctttcatc cccgccatgg cggagctgat ccagaagaag ctacagggag aagtggagaa
      301 atatcaacag ctacagaagg acttaagtaa atccatgtcg gggaggcaga aacttgaagc
      361 acaactaaca gaaaataata tcgtgaaaga ggaactggcc ctgctggatg ggtccaacgt
      421 ggtctttaaa cttctgggtc cggtgctagt caaacaggag ctgggggagg ctcgggccac
      481 agtagggaag aggctggact atatcacagc tgaaattaag cgatacgaat cccagcttcg
      541 ggatcttgag cggcagtcag agcaacagag ggagaccctt gctcagctgc agcaggagtt
      601 ccagcgggcc caggcagcaa aggcaggggc tcctggcaag gcctgacccc atggtggggg
      661 gaggggaggg gaggggaggg aatgaggcag ctctaggatc tatactgtag ctaataaaat
      721 gtaaaaacac ctgg
//
