LOCUS       NM_012393               5369 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS),
            mRNA.
ACCESSION   NM_012393
VERSION     NM_012393.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Zuo,S., Li,L., Wen,X., Gu,X., Zhuang,A., Li,R., Ye,F., Ge,S.,
            Fan,X., Fan,J., Chai,P. and Lu,L.
  TITLE     NSUN2-mediated m5 C RNA methylation dictates retinoblastoma
            progression through promoting PFAS mRNA stability and expression
  JOURNAL   Clin Transl Med 13 (5), e1273 (2023)
   PUBMED   37228185
  REMARK    GeneRIF: NSUN2-mediated m[5] C RNA methylation dictates
            retinoblastoma progression through promoting PFAS mRNA stability
            and expression.
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Ali,E.S., Sahu,U., Villa,E., O'Hara,B.P., Gao,P., Beaudet,C.,
            Wood,A.W., Asara,J.M. and Ben-Sahra,I.
  TITLE     ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De
            Novo Purine Synthesis
  JOURNAL   Mol Cell 78 (6), 1178-1191 (2020)
   PUBMED   32485148
  REMARK    GeneRIF: ERK2 Phosphorylates PFAS to Mediate Posttranslational
            Control of De Novo Purine Synthesis.
REFERENCE   3  (bases 1 to 5369)
  AUTHORS   Lu,A., Disoma,C., Zhou,Y., Chen,Z., Zhang,L., Shen,Y., Zhou,M.,
            Du,A., Zheng,R., Li,S., Alsaadawe,M., Li,S., Li,J., Wang,W.,
            Jiang,T., Peng,J. and Xia,Z.
  TITLE     Protein interactome of the deamidase
            phosphoribosylformylglycinamidine synthetase (PFAS) by LC-MS/MS
  JOURNAL   Biochem Biophys Res Commun 513 (3), 746-752 (2019)
   PUBMED   30987822
  REMARK    GeneRIF: A total of 429 proteins are newly identified to interact
            with PFAS. PFAS interacts with CAD, CCT2, PRDX1, and PHGDH.
REFERENCE   4  (bases 1 to 5369)
  AUTHORS   Mangold,C.A., Yao,P.J., Du,M., Freeman,W.M., Benkovic,S.J. and
            Szpara,M.L.
  TITLE     Expression of the purine biosynthetic enzyme phosphoribosyl
            formylglycinamidine synthase in neurons
  JOURNAL   J Neurochem 144 (6), 723-735 (2018)
   PUBMED   29337348
  REMARK    GeneRIF: Study observed FGAMS as distinct puncta throughout
            neuronal processes, where it co-localized with neurofilament heavy
            chain, tubulin, and mitochondria. Data provide insight into
            potential purine biosynthetic mechanisms utilized within neurons
            during homeostasis as well as viral infection.
REFERENCE   5  (bases 1 to 5369)
  AUTHORS   Baresova,V., Krijt,M., Skopova,V., Souckova,O., Kmoch,S. and
            Zikanova,M.
  TITLE     CRISPR-Cas9 induced mutations along de novo purine synthesis in
            HeLa cells result in accumulation of individual enzyme substrates
            and affect purinosome formation
  JOURNAL   Mol Genet Metab 119 (3), 270-277 (2016)
   PUBMED   27590927
REFERENCE   6  (bases 1 to 5369)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   7  (bases 1 to 5369)
  AUTHORS   Patterson,D., Bleskan,J., Gardiner,K. and Bowersox,J.
  TITLE     Human phosphoribosylformylglycineamide amidotransferase (FGARAT):
            regional mapping, complete coding sequence, isolation of a
            functional genomic clone, and DNA sequence analysis
  JOURNAL   Gene 239 (2), 381-391 (1999)
   PUBMED   10548741
REFERENCE   8  (bases 1 to 5369)
  AUTHORS   Brodsky,G., Barnes,T., Bleskan,J., Becker,L., Cox,M. and
            Patterson,D.
  TITLE     The human GARS-AIRS-GART gene encodes two proteins which are
            differentially expressed during human brain development and
            temporally overexpressed in cerebellum of individuals with Down
            syndrome
  JOURNAL   Hum Mol Genet 6 (12), 2043-2050 (1997)
   PUBMED   9328467
REFERENCE   9  (bases 1 to 5369)
  AUTHORS   Barnes,T.S., Bleskan,J.H., Hart,I.M., Walton,K.A., Barton,J.W. and
            Patterson,D.
  TITLE     Purification of, generation of monoclonal antibodies to, and
            mapping of phosphoribosyl N-formylglycinamide amidotransferase
  JOURNAL   Biochemistry 33 (7), 1850-1860 (1994)
   PUBMED   8110788
REFERENCE   10 (bases 1 to 5369)
  AUTHORS   Zalkin,H. and Dixon,J.E.
  TITLE     De novo purine nucleotide biosynthesis
  JOURNAL   Prog Nucleic Acid Res Mol Biol 42, 259-287 (1992)
   PUBMED   1574589
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292804.1 and BC063538.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012393.2.
            
            Summary: Purines are necessary for many cellular processes,
            including DNA replication, transcription, and energy metabolism.
            Ten enzymatic steps are required to synthesize inosine
            monophosphate (IMP) in the de novo pathway of purine biosynthesis.
            The enzyme encoded by this gene catalyzes the fourth step of IMP
            biosynthesis. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC146768.1, SRR1660809.69783.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314666.11/ ENSP00000313490.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2018              AK292804.1         12-2029
            2019-5369           BC063538.1         121-3471
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..5369
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /note="phosphoribosylformylglycinamidine synthase"
                     /db_xref="GeneID:5198"
                     /db_xref="HGNC:HGNC:8863"
                     /db_xref="MIM:602133"
     exon            1..51
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            52..272
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..4147
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /EC_number="6.3.5.3"
                     /note="FGAR amidotransferase; FGAM synthase;
                     formylglycinamide ribotide amidotransferase;
                     formylglycinamide ribotide synthetase; formylglycinamide
                     ribonucleotide amidotransferase; IKZF1/PFAS fusion;
                     phosphoribosylformylglycineamide amidotransferase"
                     /codon_start=1
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="NP_036525.1"
                     /db_xref="CCDS:CCDS11136.1"
                     /db_xref="GeneID:5198"
                     /db_xref="HGNC:HGNC:8863"
                     /db_xref="MIM:602133"
                     /translation="MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNV
                     NWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTST
                     NIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQS
                     FSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVE
                     AFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAI
                     QGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDV
                     QCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDY
                     GNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVV
                     KVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKG
                     NPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALL
                     LRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTP
                     VDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTN
                     KVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAA
                     RLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVA
                     VDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVAL
                     SPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGG
                     LVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAG
                     LHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAE
                     EERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGF
                     EVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRF
                     RKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYES
                     RWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADD
                     DGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTS
                     PWLQLFINARNWTLEGSC"
     exon            273..408
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            409..514
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            515..704
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            705..810
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            811..951
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1076
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1205
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1337
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1466
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1540
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1697
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1759
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1921
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2047
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2179
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2410
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2591
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2675
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2831
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2951
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            2952..3097
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            3098..3305
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            3306..3397
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3512
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3836
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     exon            3837..5369
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5303..5308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /note="hexamer: ACTAAA"
     polyA_site      5321
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /note="major polyA site"
     regulatory      5347..5352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /note="hexamer: AATAAA"
     polyA_site      5369
                     /gene="PFAS"
                     /gene_synonym="FGAMS; FGAR-AT; FGARAT; GATD8; PURL"
                     /note="major polyA site"
ORIGIN      
        1 aaacgcgcgc gacgattcga ggtgctctgt ggccgcgagt gcatcttcca cgaacctaat
       61 tcatctctcc agcaaaggac acatctctcc agcaaaggac acctctctcc agcaaaggac
      121 acctgcagag atgtccccag tccttcactt ctatgttcgt ccctctggcc atgagggggc
      181 agcccctgga cacactcgga ggaaactgca agggaaactg ccagagctgc agggcgtcga
      241 gactgaactg tgctacaacg tgaactggac agctgaggcc ctccccagtg ctgaggagac
      301 aaagaagctg atgtggctgt ttggttgccc cttactgctg gatgatgttg ctcgggagtc
      361 ctggctcctt cctggctcca atgacctgct gctggaggtc gggcccaggc tgaacttctc
      421 caccccaaca tccaccaaca tcgtgtcagt gtgccgcgcc actgggctgg ggcctgtgga
      481 tcgtgtggag accacccggc gctaccggct ctcgtttgcc caccccccgt cagctgaggt
      541 ggaagccatt gctctggcta ccctgcacga ccggatgaca gagcagcact tcccccatcc
      601 catccagagt ttctcccctg agagcatgcc ggaacccctc aatggcccta tcaatatact
      661 gggtgagggc cggcttgcgc tggagaaggc caaccaggag cttggtctgg ctttagactc
      721 ttgggaccta gacttctaca ccaagcgctt ccaggagcta cagcggaacc cgagcactgt
      781 ggaggccttt gacttggcgc agtccaatag cgagcacagc cgacactggt tcttcaaggg
      841 ccagctccac gtggatgggc agaagctggt gcactcactg tttgagtcca tcatgagcac
      901 ccaggaatcc tcgaacccca acaacgtcct caaattctgt gataacagca gtgcaatcca
      961 gggaaaggaa gtccgattcc tacggcctga ggaccccaca cggccaagcc gcttccagca
     1021 acagcaaggg ctgagacatg ttgtcttcac agcagagact cacaactttc ccacaggagt
     1081 atgccccttt agtggtgcaa ccactggcac agggggccgg attcgagatg tccagtgcac
     1141 aggccgcggg gcccacgtgg tggctggcac tgccggctat tgctttggaa atctgcatat
     1201 tccaggttac aatctgccct gggaggatcc aagcttccag tatcctggga attttgcccg
     1261 gcccctggag gttgccattg aagccagtaa tggagcttct gactatggca acaagtttgg
     1321 ggaaccagtg ctggctggct tcgcccgctc cttgggcctc cagctcccag acggccagcg
     1381 gcgtgagtgg atcaagccca tcatgtttag tgggggcatt gggtccatgg aagctgacca
     1441 cataagcaag gaggccccag agccaggcat ggaagttgta aaggttggag gtcccgtcta
     1501 caggattgga gttggaggtg gagctgcttc atctgtgcag gtgcagggag ataacaccag
     1561 tgacctggac tttggggctg tgcagcgagg agacccggag atggaacaga agatgaaccg
     1621 tgtgatcagg gcttgtgtgg aggcccccaa gggaaacccc atctgcagcc ttcatgatca
     1681 gggcgctggt ggcaatggca atgtcctaaa agagctgagt gacccagctg gagccatcat
     1741 ttacaccagc cgcttccagc ttggggaccc aaccctgaat gccctggaaa tctggggggc
     1801 tgagtaccag gaatcaaatg ctcttctgct gaggtccccc aaccgggact tcctgactca
     1861 tgtcagtgcc cgtgaacgtt gcccggcttg cttcgtgggc accatcactg gagaccggag
     1921 aatagtgctg gtggacgatc gggagtgtcc tgtcagaaga aatggccagg gggatgcccc
     1981 cccgacaccc ctgccaaccc ctgtggacct ggagctcgaa tgggtgctgg gcaagatgcc
     2041 tcggaaggag ttcttcctgc agaggaagcc ccccatgctg cagcctctgg ccttgccccc
     2101 agggctgagc gtgcaccagg ctctggagag ggttctgagg ctgcccgccg tggccagcaa
     2161 gcgctacctc accaataagg tggaccgctc tgtgggaggc ctggtggccc agcagcagtg
     2221 cgtggggccc ctgcaaactc ctctggcaga tgtagcggtt gtggcactga gccatgagga
     2281 gctcataggg gctgccacag ccttgggaga acagccagtc aagagcctgc tggacccaaa
     2341 agtcgccgcc cggctggccg tggccgaagc cctcaccaac ctggtgtttg ctctggtcac
     2401 tgacctccgg gatgtgaagt gtagcgggaa ctggatgtgg gcagccaagc tcccagggga
     2461 gggcgcagct ttggcggatg cctgtgaggc tatggtggca gtgatggcag ccctgggtgt
     2521 ggcagtggat ggtggcaagg actccctcag catggctgct cgggttggca ctgagaccgt
     2581 gcgggctcct gggtcactgg tcatctcagc ctatgccgtc tgcccagaca tcacagccac
     2641 tgtgacccca gacctcaagc atcctgaagg gagaggccat ctgctctatg tggctctgag
     2701 ccctgggcag caccggctcg ggggcacagc tctggcccag tgcttctccc agcttgggga
     2761 acaccctcca gacctggacc ttcctgagaa cttggtgcgg gccttcagca tcactcaggg
     2821 gctgctgaaa gaccgcctcc tctgctcagg ccacgatgtc agtgacggag gcctcgtcac
     2881 atgcctgctg gagatggcct ttgctggaaa ttgcgggcta caggtggatg tgcctgtccc
     2941 cagggttgat gtcctgtctg tgctgttcgc tgaggagcca ggcctcgtgc tggaggtgca
     3001 ggagccagac ctggcccagg tgctgaagcg ttaccgggat gctggcctcc attgcctgga
     3061 gctgggccac acaggcgagg ccgggcccca cgccatggtc cgggtgtcag tgaacggggc
     3121 tgtggttctg gaggagcctg ttggggagct gcgagccctc tgggaggaga cgagtttcca
     3181 gctggaccgg ctacaggcag agcctcgctg tgtggcagag gaggaacggg gcctgaggga
     3241 gcggatgggg cccagctatt gcctgccccc cacctttccc aaagcctccg tgccccgtga
     3301 gcctggtggt cccagccccc gagtcgccat cttgcgagag gagggcagta atggagaccg
     3361 ggagatggcc gatgccttcc acttagctgg gtttgaggta tgggacgtga ccatgcagga
     3421 cctctgctct ggggcaattg ggctggacac tttccgtggc gtggccttcg tgggcggctt
     3481 cagctatgca gatgtcctgg gctctgccaa agggtgggca gctgctgtga cctttcatcc
     3541 cagggctggg gctgagctga ggcgcttccg gaagcggcca gacaccttca gcctgggcgt
     3601 gtgtaatggc tgtcaactgc tggctctgct cggctgggtg ggaggcgacc ccaatgagga
     3661 tgctgcagag atgggccctg actcccagcc agcccggcca ggccttctgc tacgccacaa
     3721 cctgtctggg cgctacgagt ctcgctgggc cagcgtgcgt gtggggcctg ggccagccct
     3781 gatgctgcga gggatggagg gcgccgtgct gcccgtgtgg agtgcgcacg gggaaggtta
     3841 cgtagcattt tcttctccgg aactccaagc tcagattgag gccaggggct tggctccact
     3901 gcactgggct gatgatgacg ggaaccccac agagcagtac cctctgaatc ccaatgggtc
     3961 cccagggggc gtggctggca tctgctcctg tgatggccgc cacctggctg tcatgcctca
     4021 ccctgagcgg gccgttaggc cttggcagtg ggcatggcga ccccctccat ttgatactct
     4081 gaccacctcc ccctggctcc agctctttat caatgcccga aactggaccc tggaagggag
     4141 ctgctgactg gccacagggg ctcacctggg ccccatggct tttcacctaa gtgggtcctg
     4201 ccccctcccc catgaccttc aggagcaccc catattattt ccaaaaatat cttggacaga
     4261 caaggaccaa aatgccaaaa tctcagcgga ctcgataatc tgcctgctga tgttccttct
     4321 gtggctgtgt ctattttcag ttctgctcta acatggcatg ccctttctca gcccaggaaa
     4381 cagcatgtgg ttcagagaaa agagcgacaa ggaaaagtta ggactcctga ggtccgaaca
     4441 ggggcttctg ttgcccactt cacaacaccc agtgatcacc ggtgtgcaat tgcctccttg
     4501 gctctgaggg atgttttgcg ctcccttttc tcatcattgg ggttagcggg tgcagacaaa
     4561 ttcagcaata gtatgcagat cagcccctca ccacctcatt gttctcatct ggaactgaaa
     4621 ctttctggat ttctcttgaa gtgctacact gcactgaatg taaggaattg ttgcttgtgg
     4681 aagtttctca gcgtttctgg ctgtcttagg gctggcctca gaacccagca ttcctgttat
     4741 ttgcttctaa attagcagct ctcttttttt tttttttttt gaggcagtct cactctgtca
     4801 cccaggctgg agtacaatgg cgtgatctcg gcccactgca acctctgcct cctgggttca
     4861 agcaattttc ctgcctcagc ctcccgagta gctgggagta caggcacaca ccaccacacc
     4921 cagctaattt ttgtattttt agtagagata gggtttcacc atgtctccca ggctggtctc
     4981 aaactcctaa cctcaagtga ttcgcctgcc tcggcctccc aaagtgctgg gattacaggt
     5041 gggagccact acagctggcc cagcagctct gtttctgata gaggtggttg gggctctcat
     5101 ccctagatcc taacccttta gtatgctgga attctactct tcacttactg cattgactgt
     5161 tgttgattag ttattattgc aaagcactgt caccggcctc agggagttta tgtgtaatag
     5221 aattaaaaat aatagctgtg tataacactt agctcaagcc acgcatgtgt gaggcatttg
     5281 gtatgtatct gaattaattc tcactaaaat tcagcaaagg acttgatagc ccctccccgc
     5341 cttttcaata aaggatgaat gaaggttga
//
