LOCUS       NM_001127649           18626 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens peroxisomal biogenesis factor 26 (PEX26), transcript
            variant 2, mRNA.
ACCESSION   NM_001127649
VERSION     NM_001127649.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Yan,B., Cao,L., Gao,L., Wei,S., Wang,M., Tian,Y., Yang,J. and
            Chen,E.
  TITLE     PEX26 Functions as a Metastasis Suppressor in Colorectal Cancer
  JOURNAL   Dig Dis Sci 69 (1), 112-122 (2024)
   PUBMED   37957408
  REMARK    GeneRIF: PEX26 Functions as a Metastasis Suppressor in Colorectal
            Cancer.
REFERENCE   2  (bases 1 to 18626)
  AUTHORS   Dahabieh,M.S., Huang,F., Goncalves,C., Flores Gonzalez,R.E.,
            Prabhu,S., Bolt,A., Di Pietro,E., Khoury,E., Heath,J., Xu,Z.Y.,
            Remy-Sarrazin,J., Mann,K.K., Orthwein,A., Boisvert,F.M.,
            Braverman,N., Miller,W.H. and Del Rincon,S.V.
  TITLE     Silencing PEX26 as an unconventional mode to kill drug-resistant
            cancer cells and forestall drug resistance
  JOURNAL   Autophagy 18 (3), 540-558 (2022)
   PUBMED   34074205
  REMARK    GeneRIF: Silencing PEX26 as an unconventional mode to kill
            drug-resistant cancer cells and forestall drug resistance.
REFERENCE   3  (bases 1 to 18626)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 18626)
  AUTHORS   Weller,S., Cajigas,I., Morrell,J., Obie,C., Steel,G., Gould,S.J.
            and Valle,D.
  TITLE     Alternative splicing suggests extended function of PEX26 in
            peroxisome biogenesis
  JOURNAL   Am J Hum Genet 76 (6), 987-1007 (2005)
   PUBMED   15858711
  REMARK    GeneRIF: PEX26 deficiency impairs peroxisomal import of both PTS1-
            and PTS2-targeted matrix proteins.  It undergoes alternative
            splicing to produce several splice forms, including PEX26-delta,
            which rescues peroxisome biogenesis in PEX26-deficient cells.
REFERENCE   5  (bases 1 to 18626)
  AUTHORS   Fransen,M., Vastiau,I., Brees,C., Brys,V., Mannaerts,G.P. and Van
            Veldhoven,P.P.
  TITLE     Analysis of human Pex19p's domain structure by pentapeptide
            scanning mutagenesis
  JOURNAL   J Mol Biol 346 (5), 1275-1286 (2005)
   PUBMED   15713480
REFERENCE   6  (bases 1 to 18626)
  AUTHORS   Steinberg,S., Chen,L., Wei,L., Moser,A., Moser,H., Cutting,G. and
            Braverman,N.
  TITLE     The PEX Gene Screen: molecular diagnosis of peroxisome biogenesis
            disorders in the Zellweger syndrome spectrum
  JOURNAL   Mol Genet Metab 83 (3), 252-263 (2004)
   PUBMED   15542397
REFERENCE   7  (bases 1 to 18626)
  AUTHORS   Matsumoto,N., Tamura,S., Furuki,S., Miyata,N., Moser,A.,
            Shimozawa,N., Moser,H.W., Suzuki,Y., Kondo,N. and Fujiki,Y.
  TITLE     Mutations in novel peroxin gene PEX26 that cause
            peroxisome-biogenesis disorders of complementation group 8 provide
            a genotype-phenotype correlation
  JOURNAL   Am J Hum Genet 73 (2), 233-246 (2003)
   PUBMED   12851857
  REMARK    GeneRIF: degree of temperature sensitivity in pex26 cell lines is
            predictive of the clinical phenotype in patients with PEX26
            deficiency
REFERENCE   8  (bases 1 to 18626)
  AUTHORS   Matsumoto,N., Tamura,S. and Fujiki,Y.
  TITLE     The pathogenic peroxin Pex26p recruits the Pex1p-Pex6p AAA ATPase
            complexes to peroxisomes
  JOURNAL   Nat Cell Biol 5 (5), 454-460 (2003)
   PUBMED   12717447
REFERENCE   9  (bases 1 to 18626)
  AUTHORS   Vanderver,A., Tonduti,D., Schiffmann,R., Schmidt,J. and van der
            Knaap,M.S.
  TITLE     Leukodystrophy Overview - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24501781
REFERENCE   10 (bases 1 to 18626)
  AUTHORS   Steinberg,S.J., Raymond,G.V., Braverman,N.E. and Moser,A.B.
  TITLE     Zellweger Spectrum Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016027.15, CB989626.1,
            BC047320.1, AB089678.1 and CA433324.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001127649.2.
            
            Summary: This gene belongs to the peroxin-26 gene family. It is
            probably required for protein import into peroxisomes. It anchors
            PEX1 and PEX6 to peroxisome membranes, possibly to form heteromeric
            AAA ATPase complexes required for the import of proteins into
            peroxisomes. Defects in this gene are the cause of peroxisome
            biogenesis disorder complementation group 8 (PBD-CG8). PBD refers
            to a group of peroxisomal disorders arising from a failure of
            protein import into the peroxisomal membrane or matrix. The PBD
            group is comprised of four disorders: Zellweger syndrome (ZWS),
            neonatal adrenoleukodystrophy (NALD), infantile Refsum disease
            (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP).
            Alternatively spliced transcript variants have been identified for
            this gene. [provided by RefSeq, Dec 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.284023.1,
                                           SRR1660803.154118.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399744.8/ ENSP00000382648.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC016027.15        138441-138444
            5-18                CB989626.1         28-41
            19-1471             BC047320.1         1-1453
            1472-1690           AB089678.1         1184-1402
            1691-3925           AC016027.15        148912-151146
            3926-4261           CA433324.1         18-353              c
            4262-18626          AC016027.15        151483-165847
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..18626
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="peroxisomal biogenesis factor 26"
                     /db_xref="GeneID:55670"
                     /db_xref="HGNC:HGNC:22965"
                     /db_xref="MIM:608666"
     exon            1..617
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /inference="alignment:Splign:2.1.0"
     CDS             388..1305
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="isoform a is encoded by transcript variant 2;
                     peroxisome biogenesis factor 26; peroxisome assembly
                     protein 26; peroxin-26; peroxisome biogenesis disorder,
                     complementation group 8; peroxisome biogenesis disorder,
                     complementation group A"
                     /codon_start=1
                     /product="peroxisome assembly protein 26 isoform a"
                     /protein_id="NP_001121121.1"
                     /db_xref="CCDS:CCDS13750.1"
                     /db_xref="GeneID:55670"
                     /db_xref="HGNC:HGNC:22965"
                     /db_xref="MIM:608666"
                     /translation="MKSDSSTSAAPLRGLGGPLRSSEPVRAVPARAPAVDLLEEAADL
                     LVVHLDFRAALETCERAWQSLANHAVAEEPAGTSLEVKCSLCVVGIQALAEMDRWQEV
                     LSWVLQYYQVPEKLPPKVLELCILLYSKMQEPGAVLDVVGAWLQDPANQNLPEYGALA
                     EFHVQRVLLPLGCLSEAEELVVGSAAFGEERRLDVLQAIHTARQQQKQEHSGSEEAQK
                     PNLEGSVSHKFLSLPMLVRQLWDSAVSHFFSLPFKKSLLAALILCLLVVRFDPASPSS
                     LHFLYKLAQLFRWIRKAAFSRLYQLRIRD"
     exon            618..758
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /inference="alignment:Splign:2.1.0"
     exon            759..1054
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1201
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..18626
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4224..4229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="hexamer: ATTAAA"
     polyA_site      4250
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="major polyA site"
     regulatory      6184..6189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="hexamer: AATAAA"
     polyA_site      6215
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
     regulatory      8295..8300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="hexamer: AATAAA"
     polyA_site      8315
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
     regulatory      13645..13650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="hexamer: AATAAA"
     polyA_site      13669
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
     regulatory      18609..18614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
                     /note="hexamer: AATAAA"
     polyA_site      18626
                     /gene="PEX26"
                     /gene_synonym="PBD7A; PBD7B; PEX26M1T; Pex26pM1T"
ORIGIN      
        1 gcgcggctgc ggggctgggc gagcgcaaag atgtccgcgc ccgctgccgg gaggcgaggt
       61 gagtctttga tcgtaaccag gagcccggag ctgaggcagt tcctgcacgt gtcgcggggc
      121 cggagaagct agggccaggt attccaggga tgcaagaatc ctgcaaatct gacgtgtaaa
      181 ctgcttcccc agcctccagg cgagcccagc ttttgcctca gataggcccc ttccttttcc
      241 ttctcgggga atcgacctcg ggaaggggtg tgggcaaaga gatgaggact ctccctcttc
      301 gcccaggcca actcgggata tcccggagcc tctggggagg cggtcactcc gacgtctgag
      361 gacctgggcc ttggacccgg actcgttatg aagagcgatt cttcgacctc tgcagccccc
      421 ctcagggggc tcgggggacc cctgcgcagc agcgagccgg tgcgcgcggt cccggcccgg
      481 gcgccggccg tggaccttct ggaggaggcg gccgacctcc tggtggtgca cctggacttc
      541 cgggcggcgc tggagacctg cgagcgggcc tggcagagtc tggccaacca cgccgtggca
      601 gaggaacccg cgggcacctc attggaggtg aagtgctccc tgtgtgttgt ggggatccag
      661 gccctggcag aaatggatcg gtggcaagaa gtcctctcct gggtccttca gtattaccag
      721 gtccctgaaa agctaccccc caaagtcctg gagctgtgca ttcttttata cagcaaaatg
      781 caagagcctg gagctgtgct ggatgtggtg ggtgcctggc tccaagaccc agccaatcaa
      841 aaccttccag aatatggagc cttggcagaa tttcacgtgc agcgggtgct gctgcctctg
      901 ggctgcttat cggaggctga ggagctagtg gtgggctctg cagcctttgg tgaggagcgg
      961 cgactggatg tacttcaggc cattcacaca gcgaggcagc agcagaaaca ggaacactca
     1021 ggctctgagg aggcccagaa gccaaacctg gaaggctctg tctcccacaa gttcctgtca
     1081 ctaccgatgt tggttcgcca gctttgggac tctgcggtga gccacttctt ttctctgccc
     1141 ttcaaaaaga gtctcctggc tgccttgatc ctctgtctcc tggtggtgag atttgatcca
     1201 gcttcccctt cctccctgca cttcctctac aagctggccc agctcttccg ctggatccgg
     1261 aaggctgcat tttctcgcct ctaccagctc cgcatccgtg actgagggtc cctgcgcacc
     1321 acagcctctc tgctcctcac gtccgtggcc acagaagcag agcgacagag cgacacatcc
     1381 acaggcgccc ctggggaaat gggaccagcc taatctcgcg gagtgcactg tgtcttgctg
     1441 cctgggtgcc ctctcctttg caccctactt cggctggtcg cggtagatga tgtggaaaca
     1501 aagcaggacc agcaggcaca gcacctccag aacagtgccc cggatgacca gaggcccctt
     1561 gaaaaggagg ggtttgggga cagggactgt gtccatgaaa cattccatct tcttggtgaa
     1621 ggcaaggggt tggttcttca ggtcaggatg ttaatggagc tggaagttca gaaaaagcct
     1681 ggtgaagtga cccttggcct ttcacttctt gagaaacata cttctttgct gggcatggtg
     1741 actcacgcct gtaatcccag cactttggaa gggtgaggtg ggtggatcac ttgtggccag
     1801 gagttcaaaa cctgcctggc caacatggtg aaaccccatc tctactaaaa atacaaaaat
     1861 tagctagatg tggtgcacgc ctatagtcct agctacttgg gaggctgagg caggagaatc
     1921 acttgagcca gggaggcaaa agttgcagtg agctgagatt gtgctaccgc actctctcaa
     1981 aacaaacaaa aaaacatgcc ccacaggaca gtaccttaat tagctagagt agatctgaga
     2041 gggcctcttc ttgcctgcac tgtgctcccc agagctgatc taattctgta tcaataggct
     2101 gttcccatgg tcttgccatg cgcttgaagc tgcaggagcc ttctcactgt tcaggctggg
     2161 gtgtggtttt cagaaccacc gggttgacta ctgaaaacca agtgagcctt acagctctta
     2221 tcgctgggta aagtgatctt gcctggtgcc tcttggtctt cagctcaatt ttccaggttg
     2281 tcctggccaa gtcttgctct gttacgctca gtgcctctgc ccactctttc cccgccagcc
     2341 cttccctccc gccctgcaaa cttgtccctt ggctgtttca ccaggttctg cctgtatcct
     2401 tgcctccttg gtacacacca tttcaggttc ttccctttct gtgtcccatt gtcctagtta
     2461 tgttctgttg tttgtgtaat gcccaaattt ttctgctcgt gtggccttga aaagtaggcc
     2521 agcctcagag gctgctgagc tgaaaatgga actgagtaat caggtgagct ggaagggatg
     2581 tggggggcgg ggcagacggg agacatgggt tttgaaggca gtgaacaata aaaccttagg
     2641 gaggtggcac cgaggcctta gcatttgagg agctgaaatg tttcagtgtt gttttctcac
     2701 cagccacaag catcttatga gtttctgtgc aaggaagcgt agagcactgc tgtgctgtca
     2761 ggatgaagaa gggctttctg caggggctgg cctttctcta cccagaggcc aagcaggctg
     2821 ccctgcactg tgtccttggt gttgatgccc aaaatagaga aggtgcttgc tagcttttcc
     2881 ttggtacatt ttcgggggtt gcaaggcaac aagttttctt ttgttttgtt tgtttgtttg
     2941 tttgtttgtt tttgagatgg agtctctgtc acccaggctg gagtgcagtg gcgtgatctc
     3001 agctcactgc aagctccgcc tcccgggttc atgccattct cctgcctcag cctcccgagt
     3061 agctgggact acaggtgccc accacctcgc ctggctaata tttttgtatt tttagtagag
     3121 tctgggtttc actgtgttag ccgggatggt ctcgatctcc tgaacttgtg atccacccac
     3181 ctaggcctcc caaagtgctg ggattacagg tgtgagccat cgcgcccggc caacttttct
     3241 ttagaaaaat gagtaaagta ggtaaacttc acaggcccct tagaattaaa tccttagggc
     3301 atggttcaag tctgtaggaa gcaaatctat acttttttat atgggctttt aagtaaagct
     3361 tagaaccctg gggggagaaa agcagtcagg tgaggtttga gattccttct atagaatgat
     3421 ttcttaaaat gaaaatctgg gcccacttaa ctaccctaga taaaacgctt caatggcttc
     3481 ccatggctca ctggataaca tctaaattct tcatggaatt gaaggcccct atgtgctggt
     3541 tttggctaag atctcccctt tgatgtgggt cccctgttga ttatatgtgc ggccttccag
     3601 ctgcccacca tcacagctac agctcgctcc ccttggcctc tgtgccttgg ctcgcactgt
     3661 tcctactgaa ggcctagcac ctgctcttgg cctggtgagc ccctccacct tccaggctta
     3721 ctcaaagaat tctttctgcc cctccctgtg tcccgggacc tgtttcatat cgcttctgtg
     3781 gtcgtgaccc catggtcttg taattgtgaa cttctttgct gccccttcga agctggggag
     3841 tgcttgttgc agatgcatcc aagaggggta attgcgtatc aacagtggat ttgctggttg
     3901 tttctacagg gttctgaagc atgcaacata gaacagatac ctttaaattt aggggcgcta
     3961 aggaaagcag gaaccagtaa tcctcttctg tggtgggatg ggagggtggg ggagaggggt
     4021 ggaatggaga ggaaacaggc tattgttggc cagccctggg tggaggtggg tgcaagcagg
     4081 acagatagtg cctgggggtg ggggcggagg gttcgcctct aggaaggggc tagaagggat
     4141 gaggaaatag ttcatagctt cgtgcaggtg atccctattc tgcgtgtatg tgtttgcaca
     4201 tgagtgtttg tagaccatag accattaaaa aatacggaat tcaaaagtta caaaaagggt
     4261 attcttggaa aagaaagttg ttttttttta gtcttgctgt gttgcccagg ctggacctga
     4321 actcctgggc ttgagcaagt cttcccctca gcctctggag tagctgggcc tacagtgtgt
     4381 gctgccacac ccagctaata cagtctttct actcccgctc tctattcccc agtttcttgc
     4441 ccagaggcaa cccttgccag tctgtttatc cctccagata cagtctctga tagtaaactt
     4501 ttctttctct tacacaaatg atagtagtca catacactac acacactgtg ccacgtttaa
     4561 aaagaaaaaa aacaattctc ggctgggcgc ggtggcgcat gcctgtaatc ccagcacttt
     4621 gggaggccaa ggtgggtgaa tcacctgaag tcaggagttc gagatcagca tggccaacat
     4681 ggtgaaaccc catctctact aaaaatacaa aaaaaaaatg agccgggcat ggtggtgggt
     4741 acctgtaatc ccagctactc aggaggctga ggcaggagaa tcacttgaac ccgggaggca
     4801 agggttgcag tgagctgaga tcgcgccatt gcactcccac ctgggcaaca aagagtgaaa
     4861 cttggtctca gaaacgaaac aaaacacaaa aacctttctc agtcccagca tatgtggagc
     4921 agcctcattc ttcatagctg tgtgtcattc cgttgcgtga tggggtcaca gagcacagac
     4981 ctggtgccct tttccttttt aatatgtgga aacccctcca tgctttccaa agcctacaag
     5041 tacagcagcc ccaagtttag ggtgagcagc agtggtcaga gctctttact attacttttg
     5101 ggcaaacgca agccaggctg gcaaccacca ctgccgccga ggggagatac aagcaggcca
     5161 gttcacactc tggacgttca gtttctttct acatctagaa ggtggcctct gctatccact
     5221 taaagcagcc caaggaactg tcagaaagcg aaagtgatga catgaaccgc gagtagggca
     5281 cctccctgtg ccgtcgtctg ctttccttcc tccctcccag cctccctgtg ccgtcctctg
     5341 ctttcctccc tcccagcacg gaaaagccgt ggggccgatg gactttggcc tcacatccac
     5401 agatggggcc tgtgggaatt ctactgattt actctctggc ctagtcaagc aacccaccca
     5461 cggcctggcc aaggtcggcc tcaccgccct ggcctagcac ctttggaaac tgaatacatg
     5521 gagtgttaac cagaaaaatg tctggccctt ttgcctcgct gcctctggaa acctcagtgt
     5581 ttcgggctgg taggaagcta caggacagac agtgggcacg gtgcctagca gcctgtcgcg
     5641 cccccgcccg cctctgcatt cctccagcgg tggtgtacgg atccagacat tgtttgccac
     5701 ctctaggcct cttgataaaa cagcaagtat ttttcttgtc ttaaagcaat gcatgtatat
     5761 tttagaaaat attaattgca tagaaggaaa tacaggactc gtgatcccac cactcagaaa
     5821 taattactat taatacggtt gattttcttt cagctttttt ccccctaatt aattaactaa
     5881 ttcttttttt tcctgcaact cctgcttcac agactttttt ttttttttta acttggcatg
     5941 gtggctcaca cctataatcc cagcactttg ggaggccaga gtgggaggat tgcttgagcc
     6001 taggagttca aagctagcct gggcaaccta gtgggacccc atttcttttt tttggggggg
     6061 gggtgggtta taaaagccct tttataaagc caattttaaa caagaaaaaa acaaaaagtt
     6121 tacaaaagaa aaaaagatac agaaaaagaa taacttgctt catatgtccc aaaaagagaa
     6181 aaaaataaac gggacaatgc caacatgctc aacaataaag ggttcttttt cttacttttt
     6241 taatacaaaa tacaagcaaa ggatacacat acttaaaaca gagctcagga gcagacacgc
     6301 agtcctggga acccttcaat aagagcaaag caggagtttg ttttttcttt gtgcagatac
     6361 atacagagac tgggatatgt aaaaattaag tatcacaaaa gaccatcaca cgattctacc
     6421 aatgcatgtt gcatctataa ttcacgaaca tggtcaacaa gatcatgttc acttcaaccc
     6481 catttcattt aaattaaaga aaaaaacctg gccgggcgcg gtggctcacg cctgtaatcc
     6541 cagcaccttg ggaagccgag gcaggtggat cacgaggtca ggagatcgag accatcctgg
     6601 ctaacacggt gaaatcccat ctctactaaa aaaatacaaa aaattagccg ggcgtggtgg
     6661 cgggtgcctg tagccccagc tactcgggag gctgaggcag aagaatggcg tgaacccagg
     6721 aggcggagct tgcagtgagc ctagatggcg ccactgcact ccagcctggg ggacagagca
     6781 agactccgtc ttaaaaaaaa aaaaaacaaa aataaacctt ttaaataaag tggttacatt
     6841 ctaactttaa cttccttagt accatgctgc aggtttcagc actgttaagg tattgcaaga
     6901 atgcccaacc ctctggtgtc tgattgtgta tctagcaaca ttgcagtatg aagaaaagag
     6961 atgccccggg tacgacccca tttctattaa aaataaatgt aaacacccaa ccctcttggc
     7021 agcccatgca cttgagaacc tgtagaaaat ggcagtgtgc aaccagcatg gctgcgagct
     7081 cagcgtcttc actagagaga gtggaggtgg ttaggaaagg gcaggcggga aggggccatg
     7141 ttggccggac aactgcaggt gaagatggga ttgtgaagta cagtccaagg cccactcacc
     7201 aagggctgga tccaaatatg tggggttggg attctgtgac ctacttccaa ttttaatatt
     7261 gacctgagct catatttagc tctacatgtt tcccagctcg tattttccaa cagccccttt
     7321 aaaactttta gcatcctctt gtcacttttg tactatccaa ggtgggacgg acaggtgtca
     7381 gataatagca gagacttggt gaagaaatga aagcactttt gtagaagagt gtcatttcag
     7441 aaaaaagcct tttttttttg agatggagtc tcgctctgtc acccaggctg gaatgcagtg
     7501 gcgtgatctc agctcactgc aagctccgcc tcctgggttc acaccattct cctgcctcag
     7561 cctcccgagt ggctgggact ataggcgccc gccaccacgc ctggctaatt ttttggtatt
     7621 tttagtagag actgggtttc accgtgttag ccaggatggt ctcgatctcc tgacctcgtg
     7681 atccgcctcc cacagtgctg ggattacagg cgtgagccac ggcacctgga gagaaaaaag
     7741 cttctttaat gacgacctca acataattta catggagaag tttcttaggt ggaaaaccga
     7801 tttgtatggt cctcagaatg taacctacag gttcttgccc tcagaaaaat gcatgcatac
     7861 ttttatgtta caggcttatt ttccaaatgc ggtagagggc catcccttct ttggcttctt
     7921 gccttgtctc tgaccccact gactgcccct tttctgcctc catagctagt ttcttatttt
     7981 cctttggctt tttttttctt ttcttttctt ttcttctttt tttttttgag atggaatctt
     8041 gctcttgtca cctaggctgg agtgcagtgg tgcgatctcg gctcactgca ccctcctctt
     8101 gggttcaagc cagcgaggta gatattatca ttttagagac agcaaaacag actcagaggg
     8161 gctaatgtgc ccagtgcccc accgctttta aatggtagag ctgggatttg aatctaggca
     8221 tacaggtacc aggatttctg ttcttatctc ccccatagca cctggaatga ggccatccat
     8281 gtaagaggcc aagtaataaa gatttgttga atgaacttgg attttggcct taggaattag
     8341 ggaaggaaga aatagaagca gcaagagttg gtgagcaata agacccgcac cagggctgaa
     8401 gggtggaggt ggggcagggg agggctctgt gctttggcat ctgtttagta gtatcgtcgc
     8461 ccagctccct ccagagaaag agcaggaggt gttcttctca gatgtaccag caatgacaaa
     8521 gcctggcggg tgtcctcctg ggtaggtata aatacaagct ggtagtttca ttggatgact
     8581 cacacagata agccatgctc ttggttacca gtggacttag ctgggtctca aagaatgggc
     8641 tgctttctct ggaaagctgg ggagctggta aaattttttg gtcgccccac cagagacatc
     8701 ctacccattc ccagtccctc tgtgggctct gacatctctg tccggcgggc accccctgct
     8761 ctggaacatt ctgggaatgc ttcccactcc ccttttagta cttgtttgtg ttctgtcctg
     8821 ttcctggatt tcatggtctc catggtgaca agtgcttgtt ttcagaggtg ctaaagacaa
     8881 tggctaaaag atgtgctcta ttaatcctcc caggaaacat attttgcaaa agcctttttt
     8941 tttttttttt tgagaaacaa ggcctaaccc caatggagta atttgctgcg taaatgagag
     9001 acatcatggg attgtggtta agagtgtggg tcccagaccc ataatgccca agtttgtatc
     9061 tttttttttt gagacagagt ctcactctgt cgtccaagct ggagggcagt ggcatgatct
     9121 cgacacactg caacatccac ttcctaggtt caagcgattc tcttgcctca gccttccgag
     9181 tagctggaac tacaggcaca cactaccacg cctggctaat ttttgaattt ttagtagaga
     9241 cggggtttta ccatgttggc caggctggtc ttgaactctt gacctcaagt gatctgcatg
     9301 cctcacctcc caaagtgttg ggaatacagg catgagccac cacacccggc ctgagtttgt
     9361 atcttgactc acctgctttg accttcaaat aatctttcct gtgcctcagt tacttcatag
     9421 gtttgttgtg aggattaact ggtgctaatc catgtaaaac actgaaaagg tgcatagcat
     9481 gtagtgagca ggtgctagtt attattactg ttgctcttaa ttaagtgagg taaggagggg
     9541 agagttggag tggtagaacc cagcatcttg tgaacatcag accacagaaa aggcagcact
     9601 tctgattcct gttcttagcc taggaactag gctttgcaaa tggctctgaa tttttttttt
     9661 ttttttgaga tggagtctcg ctctgtcgcc caggctggag tgcagtggca cgatctcagc
     9721 tcactgcaag ctctgcctcc caggttcatg ccattcttct gcctcagcct cccgaatagc
     9781 tgggactaca ggcgcccgcc accacacccg gctaattttt tgtattttta gtagagatgg
     9841 ggtttcaccg tgttagccag gatggtctcg atctcctgac cttgtgatct gcccacctcg
     9901 gcctcccaaa gtgctgggat tacaggcgtg agccaccacg cccggccggc tctgatttct
     9961 taaagtgcta agccttttta tatattcatt tcagaccatg tttaattttc taaatatgca
    10021 atacattcat agggtttttg aaaaggcttg aaaaagtcta gtgtgaaagc agtctgtatt
    10081 agtccatttt cacactgctt tagagaagta tcagagactg ggtaattgtt gaagaaaaga
    10141 ggtttaactg actcacagtt ctgcatggct gtgaggcctc aggaaacaag cagtcatagt
    10201 ggaaggcaaa ggggaagcaa ggcacatctt acatggcagc agcagagaga atgagggggg
    10261 atgccacact ttaaaaccat cagaactcgt gagaactgac tcactatcac aagaacaaca
    10321 ggggagaaac tgttcctgtg atccagtcac ctcccaccag gtccctcctc cgacgtgggg
    10381 attacagttt gagatgagat ttgagtgggg acacagagcc aaaccatatc acctcccatc
    10441 tcttcattta gcaagttcac ctaccccaat ggcagctatg gccgttagtt tttttttttt
    10501 ttcttttttt tgagacagag cctcactctg tcgcccaggc tggagtgcag gggtgtgatc
    10561 tcggctcact gcaagctccg ccttccgggt tcacgccatt ctcctgcctc agcctcccaa
    10621 gtggctggga ctacaggctc ctgccaccgc gcccggctaa ttttttgtat ttttagtaga
    10681 gacggggtct caccgtgtta gccaggatgg tcttgatctc ctgacctcgt gatccacttg
    10741 cctcggtctc ccaaagtgct gggattacag gcatgagcca ccgtgcctgg ccacagccat
    10801 tagttttaaa tttactcttc cagaggtaaa tttaatacac caatacaaaa ccagtatgga
    10861 tatagatcct agtttcttct cctttttata aaaaaaacct gtagtatatt atgtactctg
    10921 ttttattcct tccttttttt ttcttataaa aaatacagag atggggtctt actacactga
    10981 ccaggctggt gttgaacttg cctcaagtga tcctcccatc ttgacctccc aaagtgctgg
    11041 gattataggc ataagccacc acacccagat gattttttta tttttcaaag aagtggcatg
    11101 attttgatga caataaagga caatatgtaa ttaatggtaa ttataataca gccttctaga
    11161 ggccaggtgc agtggcccat gcccgtaatc ccaccacttt gggtggccaa ggcaggtgga
    11221 tcatgaggcc aggagtttga gaccagcctg gccaacatga tgaaacccca tctcttcaaa
    11281 aaatctaaaa attagctggg tgtggtggcg tgcacctgta atctcagcta cttgggaggc
    11341 tgaggcacaa gaatcacttg aacccaggag gcggaggttg cagtgggccg agatcgagcc
    11401 actgcactcc agcctgggca acagagcgag actctgtctc acacatacac acacaaaaaa
    11461 agtataaata tatagctttc tagatatagg attggactgg ttttggttct ggctctgtta
    11521 ctaatttgcc ctataacttt gcataagtca tttaatctcg ggtccaattt ttctcacctc
    11581 caagatgggg acaaaaatta tcttccttgg ctgggtgcgg tggctcatgc ctgcaatccc
    11641 agcacttcgg gaggccaaga ccagcctggc caacatgatg aaaccccacc tctactaaaa
    11701 atacaaaaat tagctgggtg tgatggtgca cacctttaat cccagctact caggaggagg
    11761 ctgaggcagg agaatctctg gaacctggga ggcagaggtt gcagtgagct gagatcctgc
    11821 cactgcactc cagtctgggc gacagagtga gattctgtct caaaaaaaag aaaaaagaaa
    11881 aaaaaattac ctccctcatt ttctttataa tattattgtg taaaatgagt aaaagtctat
    11941 aagaaaaact ttgaaaagta ttaagtaata cacatgtgta tgtgtgcgtg tgtagacata
    12001 tatatataat atatatacac atatgcaatg gagtaatgaa aatactatat aataaatata
    12061 aaatggatta aaactatata aaatagatgc atgaaatggc tacatagata tggatgtttg
    12121 catataggca catttagctg gattaagtta gatttaaatg gtccaataga gaactgtgca
    12181 tacaattaca taggcaacca caaatcaacc ctttctctgg gctatctaaa ataatcaggt
    12241 actagaccaa aaaatgacat gctgtctgcc ttacctttta gtgatgattt gtaggaagag
    12301 gaaggtaggg gctggtgagt ggaaaagtag tagagtttgt gagggaatgt tctgtatgtc
    12361 tgaagacaaa gtctggagat tggtgggcca gaaggtgtga atctactctg aaggacaggc
    12421 aagagtccag cccagggaaa aaggtgcaga tgggtaggat ttgggtgggt acttgaatta
    12481 aaaaaatgaa tttgtgaggc aatattgctg gagtcaaaac caaaaaatca aattgaccag
    12541 ggcaaaaaat agcctggggc ctagcatggc tctgctgcca cgtaggatga ctggacctct
    12601 ttccaggttc gtgttaacag tccatactac ttatggtata cgtgaaatgc tttctattca
    12661 ttcaagcaac cttactaaaa acaccccatg aaaataaaag tctatatctt cccttcaata
    12721 tacattcata tgtgcttatt ttaagcatat atctaccaat ttgagtttgg cattcttttt
    12781 catttaatat tattacctat tcagcttcca acattatcac ttccttacta cacacatttg
    12841 tttcctagga ctgctgttaa caaattgcca caaatgtatg gcttaaaaca acagaaattt
    12901 attatctcac agctctgaag gccagaagtc agccaaagcc tctgcctctg aaggctgtag
    12961 gcaagattcc ttccttgttt cttccagctt ctgctggctc taagtgttcc ctggcctgcg
    13021 acagcatcac tccaatccct gcctcagtct tcgcttgacc ttcttcgtac ctctgtatct
    13081 tctctgccaa ggatgcatgt cactgaattt aagagccacc ctaatccagg atgatctcat
    13141 cttgaggtcc tcaattacat caagatcctt attccaaata atgtcacatt ctgagatttt
    13201 aggcaaacat atctttgggg ggccaaaatt caatccccta atccatgtta gtaaccatgc
    13261 agtgttcctt cttatttcta tgttgacctt ttcagctctt tctatttatt gttattattt
    13321 ttgagataga gtttcaatct tgttgcccag gctggagtgc aatggtgcaa tcttggctca
    13381 cggcaacctc cgcctcccag gttcaagcaa ttctcctgcc tcagcctcct gagtagctgg
    13441 tatgacaggc atgcaccacc atgcccagct aattttatat ttttagtaga ggcagggttt
    13501 cgctgtgttg gccagactgg tctcaaactc ctgatcttgg gtgatctgcc ctcctcagcc
    13561 tcccaaagtg ctgggattag aggcgtgagc caccatgcct ggccagctct ttctaactaa
    13621 aaaataataa taataataat aaaaaataaa agatgatact tgcaaaatac ctcaagtgta
    13681 cctcatttac ctcaaatata cctcatggtc cgaaagtatt tagtcagcaa tgtaccatta
    13741 tagaaaaaca catagcttgt ggctccaact ttaaaaccag aataaccaag tgaaaagtca
    13801 gtacagattc tcacttctac ttaaaaaatc aaaggtacac attgggaatt gaacagctat
    13861 gttttttctt ctttctagag atgtgacata catattttct ggtcagtaat attctaccaa
    13921 tggtattaat tcaaagcagg tagatgacca gcgatgctgt ttactacatc ctacgcatgc
    13981 tgagatagat gagtgggaca ccatctcggt aggaaagagt ttgacaggaa gaagaaattg
    14041 cagagtccct gcctgtccca gagaacctaa caaactgatc tatatggaaa ggattttcag
    14101 caaacatgca caaacacagt taatatgctg gaagaagagg atagctttta gtctatttcc
    14161 agcacacggc ctacactcac tggtacccca tacaacagga atgaatgccg acttagcatt
    14221 tttgccttca cagcgctggc aactaaaagg ctcacagata ctctgatctt taagcagata
    14281 atcatggaat tctcaacatc atatccacta ggttaggctg agggttcatc tataaaagta
    14341 tttttccaaa aataatgctt aaaagagact tctagaaaca gtgggactgt atcaggatca
    14401 acagaagact tcttgaccca aagattcaaa tatagaacta ggaattgatt ttcacatgta
    14461 gtttttaggt aaaggacatc tctttcagtt gattcctaca gccaagacta gctttgatcc
    14521 tgggagattc agaaacctca agatgggtag ctggcttgag taacatccta gataatagtc
    14581 ccagtcaaaa accccattga ggttgaagga ctggaaactg acctgtgtga aaaagagcta
    14641 tcagtgcagg aaaagactca tgctgatttt gaccttcctc tgaccctttt ccctcaactg
    14701 cttggtgggg agtagcgagg agaaggagca tgtggcaccc tcattggagg ctggagcact
    14761 gctgcagaaa gtgatctctt actcatactt caagacacag cctaagtatg accacctgta
    14821 tggacccttc cctaatgccc aggatcaaga aaagaatgtg gccaccttcc aaggctcaga
    14881 gtagctgtgc aatgacttgt gtcaaaaccc gatccagagc agcagtgatg aaatcaccct
    14941 ctcctttaag acctggcatt acaatggcct catcctgcat gtgggcaagt cagctctctg
    15001 atggatggtg caatttcctt ggtcattaac ctgaggtctg gggcctttaa agctattgag
    15061 gagccagtga atggagaatt cgacaacgcc tggcatgatt gagggtgaca catcacggca
    15121 gatgatggct gaggagacat tgcccagaag tctcatctta ggtggttgag taggaagttt
    15181 gtgaagcagg tggtgggtga gatggagtgg gtagagattc aggtatggag atgtcagtgt
    15241 ctaactttct tctagtcttt gcaatttcac aaggaaaatc actaaaatgt gcgtctcttc
    15301 atttaaaaga aaaaaaagtt catgccaaag ctgctgggac aaagaggact catgatagtg
    15361 ggttattcca cactgttgac acactgaatg tatgctggca gaagggcctc aggtggaatg
    15421 ctgggcatgg gtttgcccca cagcagtctg attcaaaagc acctgagaga tgggaggagt
    15481 actagggtta ctaaacacaa gaaggtgttt caggagaaac acatcaagcc atttgttttt
    15541 gacccttaat acttaacatg aatgatgagt tgcagaacag tcagtctttc caagatgtat
    15601 tgttctatcc tcaatagctg cagcctctcc aagccagtgg gctggtgaca tccaggatga
    15661 tcctcttcct tgcctggagg tgggatctag tgccacagct tgttcgggga agctggcata
    15721 gaagatgatt tgctcaacaa agtcaccgct aaactatcgc ttaggtctag tccccggcct
    15781 atggcaccaa gtactcctgg tagtacttgt acacagaatt tctctataat aaccagaaca
    15841 tgtcatccat ggtaatgtct gctacatatc caaccacagc atcaaattct gtctgatgaa
    15901 gggcaccagc cccttcatct ttatcatgcc taatattgtg aaggccatgt gctaaatcca
    15961 gcaatccgct ccagtaggtg tccttcagga tacttttctg tcaagtaaat acatttatta
    16021 aaaataggca tgtagccggg cgcagtggct cacacctgta atcgcagtac tttaggaggc
    16081 tgaggcgggc gaatcacgag gtcaggagtt cgagaccagc ctggccaaca cggtgaaacc
    16141 ccatctctac taaaaataca aaaaattagc tgggcatggt ggcgtgtgcc tgtaatccca
    16201 gatactcagg aggctgaggc aggagaattg cttgaaccca ggaggcagag gtggcagtga
    16261 gccgagatcg tgcgatcgtg ctattgcact ccagcctggg cgacagtgtg cgactgcatc
    16321 tcaaaaaaaa aaaaaaaaaa aaggcatgta aaagttaatc tcatggaggt agagagtaga
    16381 atgacaatta ctagaggctg ggcagggtgt gtatgtggga tgaagaggtg gcttgatgga
    16441 cacaaacata gatagaagga gtgagttcta atgcttgata gcagaatagg gtgaccattg
    16501 ttaacactgt attgggtgtt tcaaaataga cggaataaag gatttgaaat gttctcaaca
    16561 catagaaatg agaaatactc aaggtcatgg gtatcctgga taccctgagt tgatcactac
    16621 acattctatg catgtaatga aatgtcacat ctacttcaat aatatgtaca aatattattt
    16681 atcaaaaagc aggcatgtag gtgagttgat tttcttccat gtcttcaaac tcctggtaat
    16741 acttgtccac aaaatttctc tataataacc ggaacatgtc atccatgata atgtgtacta
    16801 tatatccaac cacaacatca aattctgtat cagaggcaga agagaaggac agcatgaagc
    16861 tcgattcctc caactgtcct ttggctggtg gccctccacc ctgcccacag cccggccagc
    16921 tcagccccgt ctctgtccca tacagagcca cagccacagt gagagcctgg cagcctgccc
    16981 ggaggaggct tcttggccca gcccggtcaa cacccacatg caccaccacc aactttttgc
    17041 tattgtgaat aacatagctg taaacagatt gcatatgaaa gtgctgtgaa atcaccaagg
    17101 gctgagtaga acagttaaga cgatagtagt tgttttcttt ctgggttttt ctcttgggct
    17161 ggtttttgaa agtggatcta caacattgat agagatcagt ttttctgctc ttccaacata
    17221 ctgccaggac gtggtccaga aagattgggt actttgcaat actcatagca atatgtgaag
    17281 tgattggaaa aaatgagact attcaaccag ttgtttcagt gttggttagg aggaaaatgc
    17341 ccagaaaggg gaacagttaa tcatctacac aataccaaga gcaggagggg atgaccgacg
    17401 gcttcatctt tcctagtcta tcccacacag acctttaggt gctctttttt ttcttttttt
    17461 cgctctgtcg cccatgctgg agtgcaatgg cgcgatctcg gctcaccaca acttccacct
    17521 cccaggttca agcgattctc ctgccttagc ctcctgagta gctgggatta caggcatgca
    17581 ccaccacgcc tggctaattt ttgtattttt agtagagacg gggtttctcc atgttggtct
    17641 ggctggtctc gaactcccaa cctcaggtgg tccacctgcc tcagccttcc aaagcgttgg
    17701 gattacaggc atgagccatc gcacccgcct ctttaggtgt tcttttagga gctggtagcg
    17761 gaggaaggtg gttacccatc tgtgacagag ccttggatgt cacgaggcca caggctggcc
    17821 tttcctcctg tttacaggag gtgagttttc ctgagttccc agcctcagtg ttctctgcac
    17881 agtctgtcac tgactcccgc tcccagtgct cactgcctct tgttctcact gcctcctgtt
    17941 ctcactgcct cctgtttctg ctgagttctt tgcatctgcc tgatgttggt ttaaagctcc
    18001 acccctccca ggggaatttg tcccatgatt cacctcctct ttggtgatct cttgattgcg
    18061 gctgctgcag ccatgtaaac cttaaaggcg agccaatttg tttgtgatcc agtggccaaa
    18121 atggttcacc ctcaacttcc cttaatcccc tgcagcatct gagacacatg ggtgaatgta
    18181 tgtaaggtac ttggggctaa ccaaagttaa atacatatga caagtacctg aatctagata
    18241 atctaatggt aagatatcgt ttgctctctg gacttcatgg gctcacccca gcaatggggt
    18301 gcctggaaat gaagagatct ttcttgtgtc tcatcctctg ggctcccagc ctccctcaac
    18361 atcatgacct ccatctgtaa cacccaccat cctggagcag catcctggat attggaactc
    18421 tgaagtctaa cctaagagaa caaggacaag cctctctatg aaaggtttgc ccttccagat
    18481 ccagttgagc tctgctctgg agccagactc tctgaagcat agcccagctc tagctcttac
    18541 cagttatgtg atcaggaatg agagttaatg tttctatgtt tctgattctt catttgtaaa
    18601 atgaggataa taaaaattta acttca
//
