LOCUS       NM_001172437            6639 bp    mRNA    linear   PRI 05-JUN-2024
DEFINITION  Homo sapiens paternally expressed 10 (PEG10), transcript variant 2,
            mRNA.
ACCESSION   NM_001172437
VERSION     NM_001172437.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Qin,L., Wang,J., Cheng,F., Cheng,J., Zhang,H., Zheng,H., Liu,Y.,
            Liang,Z., Wang,B., Li,C., Wang,H., Ju,Y., Tian,H. and Meng,S.
  TITLE     GPC3 and PEG10 peptides associated with placental gp96 elicit
            specific T cell immunity against hepatocellular carcinoma
  JOURNAL   Cancer Immunol Immunother 72 (12), 4337-4354 (2023)
   PUBMED   37932427
  REMARK    GeneRIF: GPC3 and PEG10 peptides associated with placental gp96
            elicit specific T cell immunity against hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Baird,L., Cannon,P., Kandel,M., Nguyen,T.V., Nguyen,A., Wong,G.,
            Murphy,C., Brownfoot,F.C., Kadife,E., Hannan,N.J., Tong,S.,
            Bartho,L.A. and Kaitu'u-Lino,T.J.
  TITLE     Paternal Expressed Gene 10 (PEG10) is decreased in early-onset
            preeclampsia
  JOURNAL   Reprod Biol Endocrinol 21 (1), 65 (2023)
   PUBMED   37464405
  REMARK    GeneRIF: Paternal Expressed Gene 10 (PEG10) is decreased in
            early-onset preeclampsia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6639)
  AUTHORS   Black,H.H., Hanson,J.L., Roberts,J.E., Leslie,S.N., Campodonico,W.,
            Ebmeier,C.C., Holling,G.A., Tay,J.W., Matthews,A.M., Ung,E.,
            Lau,C.I. and Whiteley,A.M.
  TITLE     UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain
            neuronal health in ALS
  JOURNAL   Elife 12, e79452 (2023)
   PUBMED   36951542
  REMARK    GeneRIF: UBQLN2 restrains the domesticated retrotransposon PEG10 to
            maintain neuronal health in ALS.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6639)
  AUTHORS   Lux,H., Flammann,H., Hafner,M. and Lux,A.
  TITLE     Genetic and molecular analyses of PEG10 reveal new aspects of
            genomic organization, transcription and translation
  JOURNAL   PLoS One 5 (1), e8686 (2010)
   PUBMED   20084274
  REMARK    GeneRIF: study provides new data on the genomic organization as
            well as expression and translation of PEG10, a prerequisite in
            order to study and understand the role of PEG10 in cancer,
            embryonic development and normal cell homeostasis
            GeneRIF: Evidence for translation initiation from an in-frame,
            upstream non-AUG (CUG) codon, and from the downstream AUG,
            resulting in different isoforms.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6639)
  AUTHORS   Clark,M.B., Janicke,M., Gottesbuhren,U., Kleffmann,T., Legge,M.,
            Poole,E.S. and Tate,W.P.
  TITLE     Mammalian gene PEG10 expresses two reading frames by high
            efficiency -1 frameshifting in embryonic-associated tissues
  JOURNAL   J Biol Chem 282 (52), 37359-37369 (2007)
   PUBMED   17942406
  REMARK    GeneRIF: the ORF1-2 protein of PEG10, synthesized utilizing the
            most efficient -1 frameshift mechanism yet documented in vivo, will
            have an essential function that is intrinsic to the importance of
            PEG10 in mammals
REFERENCE   6  (bases 1 to 6639)
  AUTHORS   Manktelow,E., Shigemoto,K. and Brierley,I.
  TITLE     Characterization of the frameshift signal of Edr, a mammalian
            example of programmed -1 ribosomal frameshifting
  JOURNAL   Nucleic Acids Res 33 (5), 1553-1563 (2005)
   PUBMED   15767280
  REMARK    GeneRIF: Programmed -1 ribosomal frameshifting in mouse and human
            Peg10 genes.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6639)
  AUTHORS   Lux,A., Beil,C., Majety,M., Barron,S., Gallione,C.J., Kuhn,H.M.,
            Berg,J.N., Kioschis,P., Marchuk,D.A. and Hafner,M.
  TITLE     Human retroviral gag- and gag-pol-like proteins interact with the
            transforming growth factor-beta receptor activin receptor-like
            kinase 1
  JOURNAL   J Biol Chem 280 (9), 8482-8493 (2005)
   PUBMED   15611116
  REMARK    GeneRIF: PEG10 contains two overlapping reading frames from which
            two proteins are translated by a -1 ribosomal frameshift mechanism.
REFERENCE   8  (bases 1 to 6639)
  AUTHORS   Shigemoto,K., Brennan,J., Walls,E., Watson,C.J., Stott,D.,
            Rigby,P.W. and Reith,A.D.
  TITLE     Identification and characterisation of a developmentally regulated
            mammalian gene that utilises -1 programmed ribosomal frameshifting
  JOURNAL   Nucleic Acids Res 29 (19), 4079-4088 (2001)
   PUBMED   11574691
REFERENCE   9  (bases 1 to 6639)
  AUTHORS   Ono,R., Kobayashi,S., Wagatsuma,H., Aisaka,K., Kohda,T.,
            Kaneko-Ishino,T. and Ishino,F.
  TITLE     A retrotransposon-derived gene, PEG10, is a novel imprinted gene
            located on human chromosome 7q21
  JOURNAL   Genomics 73 (2), 232-237 (2001)
   PUBMED   11318613
  REMARK    GeneRIF: PEG10 gene is imprinted, with preferential expression from
            the paternal allele.
REFERENCE   10 (bases 1 to 6639)
  AUTHORS   Volff,J., Korting,C. and Schartl,M.
  TITLE     Ty3/Gypsy retrotransposon fossils in mammalian genomes: did they
            evolve into new cellular functions?
  JOURNAL   Mol Biol Evol 18 (2), 266-270 (2001)
   PUBMED   11158386
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP250746.1, AK299837.1,
            AF216076.1, DA683494.1 and DA297295.1.
            
            On Oct 22, 2014 this sequence version replaced NM_001172437.1.
            
            Summary: This is a paternally expressed imprinted gene that is
            thought to have been derived from the Ty3/Gypsy family of
            retrotransposons. It contains two overlapping open reading frames,
            RF1 and RF2, and expresses two proteins: a shorter, gag-like
            protein (with a CCHC-type zinc finger domain) from RF1; and a
            longer, gag/pol-like fusion protein (with an additional aspartic
            protease motif) from RF1/RF2 by -1 translational frameshifting (-1
            FS). While -1 FS has been observed in RNA viruses and transposons
            in both prokaryotes and eukaryotes, this gene represents the first
            example of -1 FS in a eukaryotic cellular gene. This gene is highly
            conserved across mammalian species and retains the heptanucleotide
            (GGGAAAC) and pseudoknot elements required for -1 FS. It is
            expressed in adult and embryonic tissues (most notably in placenta)
            and reported to have a role in cell proliferation, differentiation
            and apoptosis. Overexpression of this gene has been associated with
            several malignancies, such as hepatocellular carcinoma and B-cell
            lymphocytic leukemia. Knockout mice lacking this gene showed early
            embryonic lethality with placental defects, indicating the
            importance of this gene in embryonic development. Additional
            isoforms resulting from alternatively spliced transcript variants,
            and use of upstream non-AUG (CUG) start codon have been reported
            for this gene. [provided by RefSeq, Oct 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.968662.1,
                                           SRR14038193.2364031.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 11318613
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ribosomal slippage     :: PMID: 15611116, 17942406
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                BP250746.1         1-45
            46-2631             AK299837.1         1-2586
            2632-4390           AF216076.1         2152-3910
            4391-4801           DA683494.1         26-436
            4802-5025           DA297295.1         357-580
            5026-6639           AF216076.1         4545-6158
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.3"
     gene            1..6639
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /note="paternally expressed 10"
                     /db_xref="GeneID:23089"
                     /db_xref="HGNC:HGNC:14005"
                     /db_xref="MIM:609810"
     exon            1..267
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(263..1447,1447..2616)
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /ribosomal_slippage
                     /note="protein translation is dependent on -1 ribosomal
                     frameshift; isoform 3 is encoded by transcript variant 2;
                     retrotransposon-derived protein PEG10; embryonal carcinoma
                     differentiation regulated; MEF3 like 1; ty3/Gypsy-like
                     protein; paternally expressed gene 10 protein; myelin
                     expression factor 3-like protein 1; mammalian
                     retrotransposon-derived protein 2; retrotransposon-derived
                     gag-like polyprotein; retrotransposon gag
                     domain-containing protein 3; Sushi-Ichi retrotransposon
                     homolog 1; mammalian retrotransposon-derived 2;
                     retrotransposon Gag like 2"
                     /codon_start=1
                     /product="retrotransposon-derived protein PEG10 isoform 3"
                     /protein_id="NP_001165908.1"
                     /db_xref="GeneID:23089"
                     /db_xref="HGNC:HGNC:14005"
                     /db_xref="MIM:609810"
                     /translation="MRNKRVLKTKKRRSGRGGQDPGLHPHRSEATAGRSPPTPTVTLG
                     PDCPPPPPPPPPNNNNNNNSKHTGHKSACVPNMTERRRDELSEEINNLREKVMKQSEE
                     NNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPE
                     KFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHY
                     LMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEP
                     ALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALV
                     LPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSS
                     PAGKLPGPAVEGPSATGPEIIRSPQDDASSPHLQVMLQIHLPGRHTLFVRAMIDSGAS
                     GNFIDHEYVAQNGIPLRIKDWPILVEAIDGRPIASGPVVHETHDLIVDLGDHREVLSF
                     DVTQSPFFPVVLGVRWLSTHDPNITWSTRSIVFDSEYCRYHCRMYSPIPPSLPPPAPQ
                     PPLYYPVDGYRVYQPVRYYYVQNVYTPVDEHVYPDHRLVDPHIEMIPGAHSIPSGHVY
                     SLSEPEMAALRDFVARNVKDGLITPTIAPNGAQVLQVKRGWKLQVSYDCRAPNNFTIQ
                     NQYPRLSIPNLEDQAHLATYTEFVPQIPGYQTYPTYAAYPTYPVGFAWYPVGRDGQGR
                     SLYVPVMITWNPHWYRQPPVPQYPPPQPPPPPPPPPPPPSYSTL"
     exon            268..6629
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1456..1508
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /note="recoding_pseudoknot"
                     /function="stimulates -1 ribosomal frameshifting"
     regulatory      6603..6608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /note="hexamer: AATAAA"
     polyA_site      6629
                     /gene="PEG10"
                     /gene_synonym="EDR; HB-1; Mar2; Mart2; MEF3L; RGAG3; RTL2;
                     SIRH1"
                     /note="major polyA site"
ORIGIN      
        1 ctcctcggtg caacctatat aaggctcaca gtctgcgctc ctggtacacg cgcttcaact
       61 tcggttggtg tgtgtcgaag aaacctgact gcgccctgag gagaacagcg gagaaggtcc
      121 accgagcctg gcgaaaggtc cgctgagcgg gctgtcgtcc ggagccactc cgggctgcgg
      181 agcacccagt ggagaccgcg cctggctcag gtgtgggacc ccatccttcc tgtcttcgca
      241 gaggagtcct cgcgtggtga gtatgcgaaa taagcgggtt ttgaaaacaa aaaaaagaag
      301 gagtggaaga gggggccagg atccaggcct ccatccccac agaagtgaag ctacagctgg
      361 gaggtctcct cccaccccaa ccgtcaccct gggtcccgac tgcccacctc ctcctcctcc
      421 ccctcccccc aacaacaaca acaacaacaa ctccaagcac accggccata agagtgcgtg
      481 tgtccccaac atgaccgaac gaagaaggga cgagctctct gaagagatca acaacttaag
      541 agagaaggtc atgaagcagt cggaggagaa caacaacctg cagagccagg tgcagaagct
      601 cacagaggag aacaccaccc ttcgagagca agtggaaccc acccctgagg atgaggatga
      661 tgacatcgag ctccgcggtg ctgcagcagc tgctgcccca ccccctccaa tagaggaaga
      721 gtgcccagaa gacctcccag agaagttcga tggcaaccca gacatgctgg ctcctttcat
      781 ggcccagtgc cagatcttca tggaaaagag caccagggat ttctcagttg atcgtgtccg
      841 tgtctgcttc gtgacaagca tgatgaccgg ccgtgctgcc cgttgggcct cagcaaagct
      901 ggagcgctcc cactacctga tgcacaacta cccagctttc atgatggaaa tgaagcatgt
      961 ctttgaagac cctcagaggc gagaggttgc caaacgcaag atcagacgcc tgcgccaagg
     1021 catggggtct gtcatcgact actccaatgc tttccagatg attgcccagg acctggattg
     1081 gaacgagcct gcgctgattg accagtacca cgagggcctc agcgaccaca ttcaggagga
     1141 gctctcccac ctcgaggtcg ccaagtcgct gtctgctctg attgggcagt gcattcacat
     1201 tgagagaagg ctggccaggg ctgctgcagc tcgcaagcca cgctcgccac cccgggcgct
     1261 ggtgttgcct cacattgcaa gccaccacca ggtagatcca accgagccgg tgggaggtgc
     1321 ccgcatgcgc ctgacgcagg aagaaaaaga aagacgcaga aagctgaacc tgtgcctcta
     1381 ctgtggaaca ggaggtcact acgctgacaa ttgtcctgcc aaggcctcaa agtcttcgcc
     1441 ggcgggaaac tccccggccc cgctgtagag ggaccttcag cgaccgggcc agaaataata
     1501 aggtccccac aagatgatgc ctcatctcca cacttgcaag tgatgctcca gattcatctt
     1561 ccgggcagac acaccctgtt cgtccgagcc atgatcgatt ctggtgcttc tggcaacttc
     1621 attgatcacg aatatgttgc tcaaaatgga attcctctaa gaatcaagga ctggccaata
     1681 cttgtggaag caattgatgg gcgccccata gcatcgggcc cagttgtcca cgaaactcac
     1741 gacctgatag ttgacctggg agatcaccga gaggtgctgt catttgatgt gactcagtct
     1801 ccattcttcc ctgtcgtcct aggggttcgc tggctgagca cacatgatcc caatatcaca
     1861 tggagcactc gatctatcgt ctttgattct gaatactgcc gctaccactg ccggatgtat
     1921 tctccaatac caccatcgct cccaccacca gcaccacaac cgccactcta ttatccagta
     1981 gatggataca gagtttacca accagtgagg tattactatg tccagaatgt gtacactcca
     2041 gtagatgagc acgtctaccc agatcaccgc ctggttgacc ctcacataga aatgatacct
     2101 ggagcacaca gtattcccag tggacatgtg tattcactgt ccgaacctga aatggcagct
     2161 cttcgagatt ttgtggcaag aaatgtaaaa gatgggctaa ttactccaac gattgcacct
     2221 aatggagccc aagttctcca ggtgaagagg gggtggaaac tgcaagtttc ttatgattgc
     2281 cgagctccaa acaattttac tatccagaat cagtatcctc gcctatctat tccaaattta
     2341 gaagaccaag cacacctggc aacgtacact gaattcgtac ctcaaatacc tggataccaa
     2401 acatacccca catatgccgc gtacccgacc tacccagtag gattcgcctg gtacccagtg
     2461 ggacgagacg gacaaggaag atcactatat gtacctgtga tgatcacttg gaatccacac
     2521 tggtaccgcc agcctccggt accacagtac ccgccgccac agccgccgcc tccaccacca
     2581 ccaccgccgc cgcctccatc ttacagtacc ctgtaaatac ctgtcatgtc cttcaggatc
     2641 tctgccctca aaatttattc ctgttcagct tctcaatcag tgactgtgtg ctaaatttta
     2701 ggctactgta tcttcaggcc acctgaggca catcctctct gaaacggcta tggaaggtta
     2761 gggccactct ggactggcac acatcctaaa gcaccaaaag accttcaaca ttttctgaga
     2821 gcaacagagt atttgccaat aaatgatctc tcatttttcc accttgactg ccaatctaac
     2881 taaaataatt aataagttta ctttccagcc agtcctggaa gtctgggttt tacctgccaa
     2941 aacctccatc accatctaaa ttataggctg ccaaatttgc tgtttaacat ttacagagaa
     3001 gctgatacaa acgcaggaaa tgctgatttc tttatggagg gggagacgag gaggaggagg
     3061 acatgacttt tcttgcggtt tcggtaccct ctttttaaat cactggagga ctgaggcctt
     3121 attaaggaag ccaaaattat cggtgcagtg tggaaaggct tccgtgatcc tctcgctgca
     3181 cccttagaaa cttcaccgtc ttcaaactcc atttccatgg ttctgttaat tctcaaggag
     3241 cagcaactcg actggttctc ccaggagcag gaaaaaccct tgtgacatga aacatctcag
     3301 gcctgaaaag aaagtgctct ctcagatgga ctcttgcatg ttaagactat gtcttcacat
     3361 catggtgcaa atcacatgta cccaatgact ccggctttga cacaacacct taccatcatc
     3421 atgccatgat ggcttccaca aagcattaaa cctggtaacc agagattact ggtggctcca
     3481 gcgttgttag atgttcatga aatgtgacca cctctcaatc acctttgagg gctaaagagt
     3541 agcacatcaa aaggactcca aaatcccata cccaactctt aagagatttg tcctggtact
     3601 tcagaaagaa ttttcatgag tgttcttaat tggctggaaa agcaccagct gacgttttgg
     3661 aagaatctat ccatgtgtct gcctccatat gcatctgggc atttcatctt cagtcccctc
     3721 attagactgt agcattagga tgtgtggaga gaggagaaat gatttagcac ccagattcac
     3781 actcctatgc ctggaagggg gacatctttg aagaagagga attagggctg tggacactgt
     3841 cttgaggatg tggacttcct tagtgagctc cacattactt gatggtaacc acttcaaaag
     3901 gatcagaatc cacgtaatga aaaaggtccc tctagaggat ggagctgatg tgaagctgcc
     3961 aatggatgaa aagcctcaga aagcaactca aaggactcaa agcaacggac aacacaagag
     4021 ttgtcttcag cccagtgaca cctctgatgt cccctggaag ctttgtgcta acctgggact
     4081 gcctgacttc ctttagcctg gtcccttgct actaccttga actgttttat ctaacctctc
     4141 tttttctgtt taattctttg ctactgccat tgaccctgct gcaggatttg tgtcattttc
     4201 ctgcctggtt gctgagactc cattttgctg ccacacacag agatgtaaga ggcaggcttt
     4261 aattgccaaa gcacagtttg agcagtagaa aacaacatgg tgtatatctc aaattgcctg
     4321 acatgaagag gagtctaacg gtgaagtttc acttttcatc agcatcatct ttcacatgtt
     4381 cattatcatc tgctcttatt cttgcatgtt taaacactta aaatttttag tataattttt
     4441 agtgtgtttt gaagtggtga ctaggctttc aaaaacttcc attgaattac aaagcactat
     4501 ccagttctta ttgttaaact aagtaaaaat gataagtaac atagtgtaaa atattccttt
     4561 actgtgaact tcttacaatg ctgtgaatga gaggctcctc agaactggag catttgtata
     4621 ataattcatc ctgttcatct tcaattttaa catcatatat aatttcaatt ctatcaattg
     4681 ggcctttaaa aatcatataa aaggatataa aatttgaaaa gagaaaccta attggctatt
     4741 taatccaaaa caactttttt tttccttcaa tggaatcaga aagcttgtca atcactcatg
     4801 tgttttagag taattacttt taaaatggtg catttgtgct tctgaactat tttgaagagt
     4861 cacttctgtt tacctcaagt atcaattcat cctccataca tttgaattca agttgttttt
     4921 ttgtcaaatt tacagttgtc aattgatctt caagctgcag ggtgcctaga aatgggccgt
     4981 tgtctgtagc cctggcatgt gcacacggac atttgccacc actgcaagca aaagtctgga
     5041 gaagttcacc aacgacaaga acgattaggg aaaatatgct gctgtgggtt aacaactcag
     5101 aaagtccctg atccacattt ggctgtttac taaagcttgt gattaacttt ttggcagtgt
     5161 gtactatgct ctattgctat atatgctatc tataaatgta gatgttaagg ataagtaatt
     5221 ctaaatttat tattctatag ttttgaagtt tggttaagtt tcctttcact caattgattt
     5281 attttgttgt taatcaaatt tatgttaatt ggatccttta aatttttttt ggcattttcc
     5341 aacaaaaatg gctttattca taagaaagga aaaaaatcaa tggaatttga tatctaaaga
     5401 agttagaaag ggagcaaaat aaaaaacata aaggagatag atgaattagt aagcaaatca
     5461 gtagtcgagt ttttcaaact ggcaaaatta attaattgac ttttagccca aatttacatt
     5521 gttaattaaa tcaagaagga agaagatcta agagctccca ttgataggca agcctagaga
     5581 gaactagcta aatttatcat gctaggatat tgaaacacag aaagtttaca tacatttatg
     5641 aagggtcaat ttagtttgga cagtgaggta tttgtcttag tggaaaaaag gagaattagt
     5701 ctgatcaaat cgtgaagtaa tacagtgaac ttgcaggtgc acaaaataag agggccacat
     5761 ctatatggtg cagtctggaa ttctgtttaa gtttgtaggt acctcttgga cttctgaatt
     5821 gatccagttg tcatccacca cagacatctc acatcagata cagacagttc caagattgac
     5881 aacagagaac aacctgctgg aaagacctgg gcagaaatgg agagccctgc gggaaccatg
     5941 ctacattttc atctaaagag agaatgcaca tctgatgaga ctgaaagttc tttgttgttt
     6001 tagattgtag aatggtattg aattggtctg tggaaaattg cattgctttt atttctttgt
     6061 gtaatcaagt ttaagtaata ggggatatat aatcataagc attttagggt gggagggact
     6121 attaagtaat tttaagtggg tggggttatt tagaatgtta gaataatatt atgtattaga
     6181 tatcgctata agtggacatg cgtacttact tgtaaccctt taccctataa ttgctatcct
     6241 taaagatttc aaataaactc ggagggaact gcagggagac caacttattt agagcgaatt
     6301 ggacatggat aaaaacccca gtgggagaaa gttcaaaggt gattagatta ataatttaat
     6361 agaggatgag tgacctctga taaattactg ctagaatgaa cttgtcaatg atggatggta
     6421 aattttcatg gaagttataa aagtgataaa taaaaaccct tgcttttacc cctgtcagta
     6481 gccctcctcc taccactgaa ccccattgcc cctacccctc cttctaactt tattgctgta
     6541 ttctcttcac tctatatttc tctctatttg ctaatattgc attgctgtta caataaaaat
     6601 tcaataaaga tttagtggtt aagtgcaaaa aaaaaaaaa
//
