LOCUS       NM_199203               2843 bp    mRNA    linear   PRI 28-OCT-2022
DEFINITION  Homo sapiens PEDS1-UBE2V1 readthrough (PEDS1-UBE2V1), mRNA.
ACCESSION   NM_199203
VERSION     NM_199203.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Prakash T, Sharma VK, Adati N, Ozawa R, Kumar N, Nishida Y,
            Fujikake T, Takeda T and Taylor TD.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M and
            Tokunaga K.
  TITLE     An approach based on a genome-wide association study reveals
            candidate loci for narcolepsy
  JOURNAL   Hum Genet 128 (4), 433-441 (2010)
   PUBMED   20677014
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 2843)
  AUTHORS   Thomson TM, Lozano JJ, Loukili N, Carrio R, Serras F, Cormand B,
            Valeri M, Diaz VM, Abril J, Burset M, Merino J, Macaya A, Corominas
            M and Guigo R.
  TITLE     Fusion of the human gene for the polyubiquitination coeffector UEV1
            with Kua, a newly identified gene
  JOURNAL   Genome Res 10 (11), 1743-1756 (2000)
   PUBMED   11076860
REFERENCE   4  (bases 1 to 2843)
  AUTHORS   Long M.
  TITLE     A new function evolved from gene fusion
  JOURNAL   Genome Res 10 (11), 1655-1657 (2000)
   PUBMED   11076848
  REMARK    Review article
            Erratum:[Genome Res 2001 Feb;11(2):308]
REFERENCE   5  (bases 1 to 2843)
  AUTHORS   Deng L, Wang C, Spencer E, Yang L, Braun A, You J, Slaughter C,
            Pickart C and Chen ZJ.
  TITLE     Activation of the IkappaB kinase complex by TRAF6 requires a
            dimeric ubiquitin-conjugating enzyme complex and a unique
            polyubiquitin chain
  JOURNAL   Cell 103 (2), 351-361 (2000)
   PUBMED   11057907
REFERENCE   6  (bases 1 to 2843)
  AUTHORS   Hofmann RM and Pickart CM.
  TITLE     Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in
            assembly of novel polyubiquitin chains for DNA repair
  JOURNAL   Cell 96 (5), 645-653 (1999)
   PUBMED   10089880
REFERENCE   7  (bases 1 to 2843)
  AUTHORS   Ma L, Broomfield S, Lavery C, Lin SL, Xiao W and Bacchetti S.
  TITLE     Up-regulation of CIR1/CROC1 expression upon cell immortalization
            and in tumor-derived human cell lines
  JOURNAL   Oncogene 17 (10), 1321-1326 (1998)
   PUBMED   9771976
REFERENCE   8  (bases 1 to 2843)
  AUTHORS   Xiao W, Lin SL, Broomfield S, Chow BL and Wei YF.
  TITLE     The products of the yeast MMS2 and two human homologs (hMMS2 and
            CROC-1) define a structurally and functionally conserved Ubc-like
            protein family
  JOURNAL   Nucleic Acids Res 26 (17), 3908-3914 (1998)
   PUBMED   9705497
REFERENCE   9  (bases 1 to 2843)
  AUTHORS   Sancho E, Vila MR, Sanchez-Pulido L, Lozano JJ, Paciucci R, Nadal
            M, Fox M, Harvey C, Bercovich B, Loukili N, Ciechanover A, Lin SL,
            Sanz F, Estivill X, Valencia A and Thomson TM.
  TITLE     Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating
            enzymes, in in vitro differentiation and cell cycle behavior of
            HT-29-M6 intestinal mucosecretory cells
  JOURNAL   Mol Cell Biol 18 (1), 576-589 (1998)
   PUBMED   9418904
REFERENCE   10 (bases 1 to 2843)
  AUTHORS   Rothofsky ML and Lin SL.
  TITLE     CROC-1 encodes a protein which mediates transcriptional activation
            of the human FOS promoter
  JOURNAL   Gene 195 (2), 141-149 (1997)
   PUBMED   9305758
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034423.21.
            
            On May 21, 2020 this sequence version replaced NM_199203.2.
            
            Summary: The TMEM189-UEV mRNA is an infrequent but naturally
            occurring read-through transcript of the neighboring TMEM189 and
            UBE2V1 genes. Ubiquitin-conjugating E2 enzyme variant proteins
            constitute a distinct subfamily within the E2 protein family. They
            have sequence similarity to other ubiquitin-conjugating enzymes but
            lack the conserved cysteine residue that is critical for the
            catalytic activity of E2s. The protein produced by this transcript
            has UEV1 B domains but the protein is localized to the cytoplasm
            rather than to the nucleus. The significance of this read-through
            mRNA and the function of its protein product has not yet been
            determined. [provided by RefSeq, Oct 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments and on
            experimental data in PMID:11076860.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 7335, 387521
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-207               AL034423.21        111611-111817       c
            208-327             AL034423.21        101596-101715       c
            328-419             AL034423.21        88950-89041         c
            420-564             AL034423.21        87640-87784         c
            565-777             AL034423.21        86069-86281         c
            778-926             AL034423.21        54766-54914         c
            927-1052            AL034423.21        42223-42348         c
            1053-2843           AL034423.21        39218-41008         c
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..2843
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /note="PEDS1-UBE2V1 readthrough"
                     /db_xref="GeneID:387522"
                     /db_xref="HGNC:HGNC:33521"
     exon            1..207
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1199
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /note="TMEM189-UBE2V1 readthrough transcript;
                     transmembrane protein 189-ubiquitin-conjugating enzyme E2
                     variant 1 read-through"
                     /codon_start=1
                     /product="TMEM189-UBE2V1 fusion protein"
                     /protein_id="NP_954673.2"
                     /db_xref="GeneID:387522"
                     /db_xref="HGNC:HGNC:33521"
                     /translation="MAGAENWPGQQLELDEDEASCCRWGAQHAGARELAALYSPGKRL
                     QEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWG
                     SVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPE
                     ALEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHH
                     VSPHETYFCITTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMII
                     GPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKW
                     QNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN"
     exon            208..327
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            328..419
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..564
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..777
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            778..926
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1052
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..2843
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2576..2581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /note="hexamer: AATAAA"
     polyA_site      2602
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /note="major polyA site"
     regulatory      2814..2819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
                     /note="hexamer: AATAAA"
     polyA_site      2843
                     /gene="PEDS1-UBE2V1"
                     /gene_synonym="CROC-1B; CROC1B; KUA-UEV; TMEM189-UBE2V1"
ORIGIN      
        1 attgggcggc gtgatctcgc cgcggttccg cggccctgcc gccgccgccg ccagcagagc
       61 gcaccgggcc gatcgggcga gtggccatgg cgggcgccga gaactggccg ggccagcagc
      121 tggagctgga cgaggacgag gcgtcttgtt gccgctgggg cgcgcagcac gccggggccc
      181 gcgagctggc tgcgctctac tcgccaggca agcgcctcca ggagtggtgc tctgtgatcc
      241 tgtgcttcag cctcatcgcc cacaacctgg tccatctcct gctgctggcc cgctgggagg
      301 acacacccct cgtcatactc ggtgttgttg caggggctct cattgctgac ttcttgtctg
      361 gcctggtaca ctggggtgct gacacatggg gctctgtgga gctgcccatt gtggggaagg
      421 ctttcatccg acccttccgg gagcaccaca ttgacccgac agctatcaca cggcacgact
      481 tcatcgagac caacggggac aactgcctgg tgacactgct gccgctgcta aacatggcct
      541 acaagttccg cacccacagc cctgaagccc tggagcagct atacccctgg gagtgcttcg
      601 tcttctgcct gatcatcttc ggcaccttca ccaaccagat ccacaagtgg tcgcacacgt
      661 actttgggct gccacgctgg gtcaccctcc tgcaggactg gcatgtcatc ctgccacgta
      721 aacaccatcg catccaccac gtctcacccc acgagaccta cttctgcatc accacaggag
      781 taaaagtccc tcgcaatttc cgactgttgg aagaactcga agaaggccag aaaggagtag
      841 gagatggcac agttagctgg ggtctagaag atgacgaaga catgacactt acaagatgga
      901 cagggatgat aattgggcct ccaagaacaa tttatgaaaa ccgaatatac agccttaaaa
      961 tagaatgtgg acctaaatac ccagaagcac ccccctttgt aagatttgta acaaaaatta
     1021 atatgaatgg agtaaatagt tctaatggag tggtggaccc aagagccata tcagtgctag
     1081 caaaatggca gaattcatat agcatcaaag ttgtcctgca agagcttcgg cgcctaatga
     1141 tgtctaaaga aaatatgaaa ctccctcagc cgcccgaagg acagtgttac agcaattaat
     1201 caaaaagaaa aaccacaggc ccttcccctt ccccccaatt cgatttaatc agtcttcatt
     1261 ttccacagta gtaaattttc tagatacgtc ttgtagacct caaagtaccg gaaaggaagc
     1321 tcccattcaa aggaaattta tcttaagata ctgtaaatga tactaatttt ttgtccattt
     1381 gaaatatata agttgtgcta taacaaatca tcctgtcaag tgtaaccact gtccacgtag
     1441 ttgaacttct gggatcaaga aagtctattt aaattgattc ccatcataac tggtggggca
     1501 catctaactc aactgtgaaa agacacatca cacaatcacc ttgctgctga ttacacggcc
     1561 tggggtctct gccttctccc cttaccctcc cgcctcccac cctccctgca acaacagccc
     1621 tctagcctgg ggggcttgtt agagtagatg tgaaggtttc aggtcgcagc ctgtgggact
     1681 actgctaggt gtgtggggtg tttcgcctgc acccctggtt tctttaagtc ttaagtgatg
     1741 ccccttccaa accatcatcc tgtccccacg ctcctccact cccgcccttg gccgaagcat
     1801 agattgtaac ccctccactc ccctctgaga ttggccttcg gtgaggaatt cagggctttc
     1861 cccatatctt ctctccccca cctttatcga ggggtgctgc tttttctccc tcctcctcaa
     1921 gttccttttt gcaccgtcac cacccaacac cttccatgac acttccttgc tttggccaga
     1981 agccatcagg taaggttgga aagagcctct gacctccctt gtttagtttt ggaaccatac
     2041 tcactcactc tccaccagcc tgggaaatga atattgggtc ctcagccctg ccaccctctg
     2101 ctgtcatcag ctgatgcatt gtttttagct caggttttga taaggtgaaa agaatagtca
     2161 ccagggttac tcagacctgc cagctctcgg agtccttggt ggttgaactt ggagaaagac
     2221 cgcatgaaga tacttgtaag cacacatgat ccctctgaat tgttttactt tcctgtaact
     2281 gcttttgctt ttaaaaattg aagaagtttt aaacagggct ttcatttggt catccttgca
     2341 atccattggg gtctagtttg gaatctgaca actggaacaa aaagaacctt gaatccggtg
     2401 catgccttgg ttttggtgct gctgctgctt cccaagatcc tcagcaggga ttaagaagga
     2461 acccggtgtg cacagcagat ccccgaaatt ggtgggcttg acctcctggc aaattgctgc
     2521 gtctttccac ttgctgttca ggaccactaa atgctgaaat gtggatgcat accgaaataa
     2581 aagcaattca ttgtgtacta aaggtttttt tttttttttt aatttagtat ttgtgtaaaa
     2641 ccaccttttg aagcagcaac tatcaagtct gaaaagcaat tgatgtttcc attaatcttt
     2701 ttctgggggg aaaaccttag ttctaaggat ttaacatcct gtaagtgaag tttaacataa
     2761 cagtattcca taagcagcct ttttattgtc agaccattgc ctgattttaa tataataaaa
     2821 aaaaagtgtg cgttaatatt taa
//
