LOCUS       NM_001385026           12003 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens pseudopodium enriched atypical kinase 1 (PEAK1),
            transcript variant 1, mRNA.
ACCESSION   NM_001385026
VERSION     NM_001385026.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Zhu,J., Wang,F., Weng,Y. and Zhao,J.
  TITLE     Exosome-delivered circSATB2 targets the miR-330-5p/PEAK1 axis to
            regulate proliferation, migration and invasion of lung cancer cells
  JOURNAL   Thorac Cancer 13 (21), 3007-3017 (2022)
   PUBMED   36148757
  REMARK    GeneRIF: Exosome-delivered circSATB2 targets the miR-330-5p/PEAK1
            axis to regulate proliferation, migration and invasion of lung
            cancer cells.
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Zuidema,A., Atherton,P., Kreft,M., Hoekman,L., Bleijerveld,O.B.,
            Nagaraj,N., Chen,N., Fassler,R. and Sonnenberg,A.
  TITLE     PEAK1 Y635 phosphorylation regulates cell migration through
            association with Tensin3 and integrins
  JOURNAL   J Cell Biol 221 (8) (2022)
   PUBMED   35687021
  REMARK    GeneRIF: PEAK1 Y635 phosphorylation regulates cell migration
            through association with Tensin3 and integrins.
REFERENCE   3  (bases 1 to 12003)
  AUTHORS   Wang,X., Zheng,Y. and Wang,Y.
  TITLE     PEAK1 promotes invasion and metastasis and confers drug resistance
            in breast cancer
  JOURNAL   Clin Exp Med 22 (3), 393-402 (2022)
   PUBMED   34554318
  REMARK    GeneRIF: PEAK1 promotes invasion and metastasis and confers drug
            resistance in breast cancer.
REFERENCE   4  (bases 1 to 12003)
  AUTHORS   Li,P., Zhao,H., Liu,X., Huang,Y. and Chen,D.
  TITLE     Clinical Significance of PEAK1 Expression and BRAF V600E Mutation
            in Papillary Thyroid Cancer
  JOURNAL   Br J Biomed Sci 79, 10268 (2022)
   PUBMED   35996515
  REMARK    GeneRIF: Clinical Significance of PEAK1 Expression and BRAF V600E
            Mutation in Papillary Thyroid Cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12003)
  AUTHORS   Pan,M., Yin,X. and Huang,Y.C.
  TITLE     Pseudopodium enriched atypical kinase 1(PEAK1) promotes invasion
            and of melanoma cells by activating JAK/STAT3 signals
  JOURNAL   Bioengineered 12 (1), 5045-5055 (2021)
   PUBMED   34365903
  REMARK    GeneRIF: Pseudopodium enriched atypical kinase 1(PEAK1) promotes
            invasion and of melanoma cells by activating JAK/STAT3 signals.
REFERENCE   6  (bases 1 to 12003)
  AUTHORS   Bristow,J.M., Reno,T.A., Jo,M., Gonias,S.L. and Klemke,R.L.
  TITLE     Dynamic phosphorylation of tyrosine 665 in pseudopodium-enriched
            atypical kinase 1 (PEAK1) is essential for the regulation of cell
            migration and focal adhesion turnover
  JOURNAL   J Biol Chem 288 (1), 123-131 (2013)
   PUBMED   23105102
  REMARK    GeneRIF: phosphorylation of tyrosine 665 in pseudopodium-enriched
            atypical kinase 1 (PEAK1) is essential for the regulation of cell
            migration and focal adhesion turnover
REFERENCE   7  (bases 1 to 12003)
  AUTHORS   Boudeau,J., Miranda-Saavedra,D., Barton,G.J. and Alessi,D.R.
  TITLE     Emerging roles of pseudokinases
  JOURNAL   Trends Cell Biol 16 (9), 443-452 (2006)
   PUBMED   16879967
  REMARK    Review article
REFERENCE   8  (bases 1 to 12003)
  AUTHORS   Zody,M.C., Garber,M., Sharpe,T., Young,S.K., Rowen,L., O'Neill,K.,
            Whittaker,C.A., Kamal,M., Chang,J.L., Cuomo,C.A., Dewar,K.,
            FitzGerald,M.G., Kodira,C.D., Madan,A., Qin,S., Yang,X., Abbasi,N.,
            Abouelleil,A., Arachchi,H.M., Baradarani,L., Birditt,B., Bloom,S.,
            Bloom,T., Borowsky,M.L., Burke,J., Butler,J., Cook,A.,
            DeArellano,K., DeCaprio,D., Dorris,L. 3rd, Dors,M., Eichler,E.E.,
            Engels,R., Fahey,J., Fleetwood,P., Friedman,C., Gearin,G.,
            Hall,J.L., Hensley,G., Johnson,E., Jones,C., Kamat,A., Kaur,A.,
            Locke,D.P., Madan,A., Munson,G., Jaffe,D.B., Lui,A., Macdonald,P.,
            Mauceli,E., Naylor,J.W., Nesbitt,R., Nicol,R., O'Leary,S.B.,
            Ratcliffe,A., Rounsley,S., She,X., Sneddon,K.M., Stewart,S.,
            Sougnez,C., Stone,S.M., Topham,K., Vincent,D., Wang,S.,
            Zimmer,A.R., Birren,B.W., Hood,L., Lander,E.S. and Nusbaum,C.
  TITLE     Analysis of the DNA sequence and duplication history of human
            chromosome 15
  JOURNAL   Nature 440 (7084), 671-675 (2006)
   PUBMED   16572171
REFERENCE   9  (bases 1 to 12003)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   10 (bases 1 to 12003)
  AUTHORS   Manning,G., Whyte,D.B., Martinez,R., Hunter,T. and Sudarsanam,S.
  TITLE     The protein kinase complement of the human genome
  JOURNAL   Science 298 (5600), 1912-1934 (2002)
   PUBMED   12471243
  REMARK    GeneRIF: Also known as SgK269. Contains a divergent and possibly
            inactive eukaryotic protein kinase domain. Has one human paralog,
            known as SgK223 or pragmin. These two constitute the NKF3 subfamily
            of protein kinases, found in vertebrates and echinoderms
            Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090984.5, AC060773.10,
            AC087465.5 and AC107883.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a non-receptor tyrosine kinase that is a
            member of the new kinase family three (NFK3) family. In migrating
            cells, the encoded protein is associated with the actin
            cytoskeleton and focal adhesions and promotes developing focal
            adhesion elongation. This protein may play a role in the regulation
            of cell migration, proliferation and cancer metastasis. [provided
            by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1, 2, 4,
            and 5 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB082533.1, SRR14038193.1169575.1
                                    [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682557.1/ ENSP00000507603.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC090984.5         94977-95075         c
            100-162             AC060773.10        47828-47890
            163-244             AC060773.10        126549-126630
            245-342             AC060773.10        127998-128095
            343-490             AC060773.10        129023-129170
            491-650             AC087465.5         146445-146604       c
            651-3901            AC087465.5         72868-76118         c
            3902-4095           AC087465.5         52581-52774         c
            4096-4841           AC087465.5         27083-27828         c
            4842-12003          AC107883.6         7036-14197
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.3"
     gene            1..12003
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /note="pseudopodium enriched atypical kinase 1"
                     /db_xref="GeneID:79834"
                     /db_xref="HGNC:HGNC:29431"
                     /db_xref="MIM:614248"
     exon            1..99
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            100..162
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            163..244
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            245..342
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            343..490
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            491..650
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            651..3901
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     CDS             765..6005
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /EC_number="2.7.10.2"
                     /note="isoform a is encoded by transcript variant 1;
                     tyrosine-protein kinase SgK269; NKF3 kinase family member;
                     sugen kinase 269; inactive tyrosine-protein kinase PEAK1"
                     /codon_start=1
                     /product="inactive tyrosine-protein kinase PEAK1 isoform
                     a"
                     /protein_id="NP_001371955.1"
                     /db_xref="CCDS:CCDS42062.1"
                     /db_xref="GeneID:79834"
                     /db_xref="HGNC:HGNC:29431"
                     /db_xref="MIM:614248"
                     /translation="MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNVK
                     TNANHSNNHRIRNTGNFRPPVAKKPTIAVKPTMIVADGQSICGELSIQEHCENKPVII
                     GWNRNRAALSQKPLNNNNEDDEGISHVPKPYGNNDSAKKMSDNNNGLTEVLKEIAGLD
                     TAPQIRGNETNSRETFLGRINDCYKRSLERKLPPSCMIGGIKETQGKHVILSGSTEVI
                     SNEGGRFCYPEFSSGEESEEDVLFSNMEEEHESWDESDEELLAMEIRMRGQPRFANFR
                     ANTLSPVRFFVDKKWNTIPLRNKSLQRICAVDYDDSYDEILNGYEENSVVSYGQGSIQ
                     SMVSSDSTSPDSSLTEESRSETASSLSQKICNGGLSPGNPGDSKDMKEIEPNYESPSS
                     NNQDKDSSQASKSSIKVPETHKAVLALRLEEKDGKIAVQTEKEESKASTDVAGQAVTI
                     NLVPTEEQAKPYRVVNLEQPLCKPYTVVDVSAAMASEHLEGPVNSPKTKSSSSTPNSP
                     VTSSSLTPGQISAHFQKSSAIRYQEVWTSSTSPRQKIPKVELITSGTGPNVPPRKNCH
                     KSAPTSPTATNISSKTIPVKSPNLSEIKFNSYNNAGMPPFPIIIHDEPTYARSSKNAI
                     KVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSVISHTYEEIETESKVPDNTTSKT
                     TDCLQTKGFSNSTEHKRGSVAQKVQEFNNCLNRGQSSPQRSYSSSHSSPAKIQRATQE
                     PVAKIEGTQESQMVGSSSTREKASTVLSQIVASIQPPQSPPETPQSGPKACSVEELYA
                     IPPDADVAKSTPKSTPVRPKSLFTSQPSGEAEAPQTTDSPTTKVQKDPSIKPVTPSPS
                     KLVTSPQSEPPAPFPPPRSTSSPYHAGNLLQRHFTNWTKPTSPTRSTEAESVLHSEGS
                     RRAADAKPKRWISFKSFFRRRKTDEEDDKEKEREKGKLVGLDGTVIHMLPPPPVQRHH
                     WFTEAKGESSEKPAIVFMYRCDPAQGQLSVDQSKARTDQAAVMEKGRAENALLQDSEK
                     KRSHSSPSQIPKKILSHMTHEVTEDFSPRDPRTVVGKQDGRGCTSVTTALSLPELERE
                     DGKEDISDPMDPNPCSATYSNLGQSRAAMIPPKQPRQPKGAVDDAIAFGGKTDQEAPN
                     ASQPTPPPLPKKMIIRANTEPISKDLQKSMESSLCVMANPTYDIDPNWDASSAGSSIS
                     YELKGLDIESYDSLERPLRKERPVPSAANSISSLTTLSIKDRFSNSMESLSSRRGPSC
                     RQGRGIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFG
                     VDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAVR
                     QSLAVHFNIQQDCGHFLAEVPNRLLPWEDPDDPEKDEDDMEETEEDAKGETDGKNPKP
                     CSEAASSQKENQGVMSKKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDLYERQVC
                     LLLLQLCSGLEHLKPYHVTHCDLRLENLLLVHYQPGGTAQGFGPAEPSPTSSYPTRLI
                     VSNFSQAKQKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFD
                     ENPELKEREYTRADLPRIPFRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLW
                     GPREDLFQTFTACPSLVQRNTLLQNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQ
                     YLAFATTDSLSCIVKILQHR"
     exon            3902..4095
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            4096..4841
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     exon            4842..12003
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11974..11979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /note="hexamer: AATAAA"
     polyA_site      12003
                     /gene="PEAK1"
                     /gene_synonym="SGK269"
                     /note="major polyA site"
ORIGIN      
        1 agtcagtgtc agttgctcgg cggcggcggc cgtggtcacc aggaagggga cgggacggac
       61 ggtgatggtg gtcgccgcgg cggcgtgtgc gcccctcagg tatctgccca aactggaact
      121 tatgctgtag acatttcttt gtggtttatc taaaaaccaa aggaaaagaa tacttgagat
      181 ccatttgtaa ggaaagaaaa ggaagcaaca acataatgcc cagagttgga gccaattaaa
      241 ccaggctgga gtgcggtggc gtgatcttgg ctcactgcaa cctctgcctt ccgggttcga
      301 gtaattctcc tgcctcagcc tcccaagcag ctgggtctac agtgagacta attacatcct
      361 tcaatgaagt accattttgt gagaaagtga gaattcaatg atttatgaaa aaatccaaga
      421 aggagaaaca aacattttct aatatgaaag tagtaatgac tacagctata taaataacag
      481 taacaaagag taaacattgc tatcttcaga aattaaggaa tgagaggcaa ggaagaaagg
      541 acattcatct tggaaagacc caaagcacct tgaaatttgg aaggaatgtt ttgctagctg
      601 ctaaaaccac ctttgtcacc agaatgaatg aagtgcttca gatttaaata attgatcatt
      661 cttagaagag ctgctgtaac acatagggga agtgaaaact aacagatgaa agttttgtca
      721 tgcactgaaa gaaaagcatt gtctgtgaag ttctattttt aaaaatgtct gcttgtaaca
      781 cctttactga acatgtttgg aaacctggtg aatgcaagaa ttgctttaaa cctaaaagtt
      841 tgcaccagct tcccccagac cctgagaagg cacccatcac ccatggcaat gtgaaaacta
      901 atgccaatca cagtaacaac caccgcatca ggaacacggg caatttccgg cctcctgtgg
      961 ctaaaaaacc cactatagct gtgaagccca ctatgatagt ggcagatggg caaagtatat
     1021 gtggtgagct tagcatccaa gaacactgtg agaacaaacc tgtcatcata gggtggaacc
     1081 gaaacagagc tgccttgagt cagaaaccac ttaacaataa taatgaagat gatgaaggaa
     1141 ttagccatgt tcctaagcct tatggcaata atgatagtgc aaagaagatg tcagataaca
     1201 ataatggact aactgaagtg ttaaaggaga tagcaggctt ggatactgcc cctcagataa
     1261 gaggaaatga aacaaactcc agagaaacat tcttgggaag aataaatgat tgctataaac
     1321 gatcattgga aagaaagctt ccaccaagtt gcatgatagg tgggataaag gaaactcagg
     1381 gcaagcatgt tattctgagt gggagcacag aagtgattag taatgaaggg ggccggttct
     1441 gttacccaga gttttccagt ggcgaggaga gtgaagagga tgtacttttc agtaacatgg
     1501 aggaggagca cgagagttgg gatgagagtg atgaagagct gttggccatg gagattcgca
     1561 tgagagggca acctcgcttt gccaacttca gagcaaacac attgtctcct gttcgattct
     1621 ttgtggacaa aaaatggaat accatccccc tgcgaaacaa gtctctgcag agaatctgtg
     1681 ctgtggacta tgatgacagc tatgatgaaa tcctgaatgg ttatgaggaa aattctgtgg
     1741 tctcttatgg acaaggaagc attcagagca tggtgtcatc tgactccaca tcaccagatt
     1801 cttctttaac agaagaatca cgttctgaga cagccagtag tttatcccag aagatttgta
     1861 atgggggatt atctcctggt aacccaggag attctaagga catgaaggaa attgagccca
     1921 attatgaaag tccctctagt aataatcagg ataaagattc atcacaggct tccaaaagct
     1981 caataaaagt tccagagacc cacaaagcag tccttgctct ccgattagaa gagaaagatg
     2041 gcaagattgc tgtacaaact gagaaggaag aaagtaaagc ctctacagat gttgctgggc
     2101 aagcagtaac cataaacctt gtccccacag aagagcaagc aaaaccttac cgagttgtga
     2161 acctggaaca gccattgtgc aagccatata ctgtcgtgga tgtgtcagca gccatggcca
     2221 gtgagcacct cgagggccct gttaacagcc ccaagacaaa aagctcatcc tctactccaa
     2281 actctccagt tacatcatct tcattgacac caggacaaat aagtgcccat ttccaaaaat
     2341 ccagtgcaat tcgataccaa gaagtatgga cttctagcac cagtccacga caaaagatac
     2401 ctaaagtaga actaattact agtggaactg gaccaaatgt tcctccaagg aaaaactgtc
     2461 acaaatcagc acctacatca cccacagcta caaacatttc ctccaaaacc atccctgtta
     2521 agtcacctaa tttgtctgaa attaaattta atagttataa caatgctggt atgccacctt
     2581 ttccaattat cattcatgac gagccaactt atgctcggag ttccaaaaat gctatcaaag
     2641 ttcccattgt tatcaatcca aatgcatatg acaatctagc tatctacaaa agttttctgg
     2701 gaacaagtgg agaactctca gtgaaggaaa aaaccacaag tgtaataagc catacttatg
     2761 aagaaataga aacagaaagc aaagtgcctg ataacaccac tagcaaaacc actgactgtc
     2821 ttcaaactaa agggttttca aacagcacag agcataaaag gggctcagtg gctcagaagg
     2881 ttcaagagtt taacaactgt ctcaacagag gtcagtcttc accacagaga agctatagtt
     2941 ccagccacag ctccccagca aagatccaga gagccactca agagcctgtg gccaaaatag
     3001 aaggcactca ggagtctcag atggtgggca gcagcagcac cagagagaaa gcaagcacag
     3061 tgctttctca gattgtggct tcaatccaac ccccacagtc tcctccagaa acacctcaat
     3121 ctggccctaa agcttgcagt gtggaagagc tttatgccat tcctccagat gctgatgttg
     3181 ctaagagcac acctaagagt acgccagtcc ggcccaaatc tctctttaca tctcagccta
     3241 gtggtgaggc tgaagcacct cagaccacag acagtcctac caccaaagta cagaaagacc
     3301 catccataaa gccagtcacc ccctctccct ccaaattagt gactagcccc caaagtgagc
     3361 caccagctcc ctttcccccg ccacgctcta cttcttctcc ttaccatgca ggtaaccttt
     3421 tgcagaggca tttcaccaac tggaccaagc caaccagccc taccaggtca acagaagctg
     3481 aatcagtttt gcactctgaa ggcagcaggc gggcagctga tgcaaaacct aagcgctgga
     3541 tatcatttaa aagcttcttc cgccgtcgga aaacagatga ggaggatgac aaagagaaag
     3601 agcgagagaa agggaaactg gtgggcctgg atggcacagt cattcacatg ctgcctcctc
     3661 ctccagttca gcgccatcac tggttcacag aggcgaaagg agagtccagt gagaaaccag
     3721 ccattgtctt catgtacagg tgcgaccctg ctcaaggcca gctcagtgtg gatcagagca
     3781 aggctaggac agaccaggca gcagtcatgg agaagggtag agcagagaat gcattactac
     3841 aggactcaga gaagaagagg agtcattctt ctccatcaca gattcctaaa aagattctca
     3901 gtcacatgac ccatgaagta acagaggatt tttctcctcg ggatccaaga actgttgttg
     3961 ggaagcaaga tggcaggggc tgcacttcag tcacaacagc attgtcccta cctgaactgg
     4021 aaagggaaga tggaaaagaa gacatttcag atcctatgga cccgaaccct tgtagtgcaa
     4081 catacagcaa cttagggcaa tctagagcag ccatgatacc tcccaagcag ccacgacagc
     4141 ccaagggagc tgtggacgat gccatcgcct ttggagggaa aacagaccaa gaagcaccca
     4201 atgcttccca acctacacca cccccactgc caaagaagat gatcataaga gccaatacag
     4261 agccaatctc caaggacctc caaaaatcca tggaaagtag tctttgtgtc atggctaatc
     4321 ccacctatga tatcgacccc aactgggatg ccagcagtgc tggttcttcc atcagctatg
     4381 aactcaaagg actggacatt gagtcttatg actccttgga aaggcctttg cgcaaggaga
     4441 gacctgtccc ctcagcagca aacagcattt ccagcttaac cactctcagt attaaggata
     4501 gattttccaa cagcatggaa tccctctcca gccggcgtgg gccctcttgc agacagggcc
     4561 gaggcatcca gaagccgcag agacaagcac tttatcgagg acttgagaat cgggaggaag
     4621 tagtgggtaa aatccgaagc cttcatacag atgccttgaa gaaactggct gttaaatgcg
     4681 aagacctttt catggctggg cagaaagacc agctccgttt tggagtggac agctggtcag
     4741 acttcaggct aaccagtgac aaaccatgtt gtgaggcagg tgatgcggtt tactatactg
     4801 cttcatatgc aaaagatcca cttaataact atgcagtcaa gatctgtaag agcaaagcta
     4861 aagaatctca gcagtattat cacagcttgg ctgtccggca gagtctggct gtccatttta
     4921 acattcagca ggactgtggt catttccttg ctgaagtccc taaccgtctg cttccctggg
     4981 aggatccaga tgaccctgaa aaggatgagg atgacatgga agagactgaa gaagacgcca
     5041 aaggagaaac ggatgggaaa aacccaaagc cctgttctga agcagcatca tcccagaaag
     5101 agaatcaggg agtcatgagc aagaagcaga ggagccacgt tgtggtcatc accagggagg
     5161 ttccatgtct tactgtggct gattttgtgc gagactctct ggcccagcat gggaaaagcc
     5221 ctgatttgta tgagaggcag gtgtgtctgc tgctcttaca gctatgctct ggtcttgagc
     5281 acctcaaacc ctaccatgtc actcactgcg atctacgcct agagaacctg ctacttgtcc
     5341 actaccagcc tggggggact gcccaaggct ttgggcctgc agagcccagc cccacctcat
     5401 cttatcccac taggcttata gtgagcaact tctctcaggc caagcagaag agccatctgg
     5461 tggaccccga gatcctccgg gaccagtctc gccttgcccc agagatcata acagctaccc
     5521 agtataaaaa gtgtgatgag ttccagacag gcatcctcat ctatgagatg ctgcacctac
     5581 ccaacccctt tgatgagaac ccagagctga aggagaggga atacacacga gcagacctgc
     5641 ctcgcatccc attccgctcc ccctactccc ggggtctgca gcagctggcc agctgcctcc
     5701 tgaatcccaa cccttctgag cggatcctca tttcagacgc caaaggcatc ctccagtgtc
     5761 tgctctgggg cccccgcgaa gatctcttcc agactttcac cgcctgccct agcctagtac
     5821 agaggaacac cctgctccaa aactggctag acatcaagcg aacactgctc atgatcaagt
     5881 ttgctgagaa gtccctggac agggaaggtg gaatcagcct tgaggactgg ctttgtgctc
     5941 agtatttggc ttttgccact acagactccc tcagttgtat tgtgaaaatt ctgcagcacc
     6001 gttaatgtgt acctggatga tgcttttact acatcttcac cttcttcatg tcacccatgc
     6061 acttcccctc cctagtgctc actccaaatt caaggaaaga agaaaggcaa acctctatcc
     6121 ctgtccgtga acaagtgggt ctattcagaa aggttatcca ctgccccaga ttgaatgaga
     6181 agctgagtct gtcttaactt tacagaaatt cagctgcaca aacttgcagt tgccttccct
     6241 tgaaacacct tctatcctaa tagtcccata aatgcaggta aaaatgaaaa tccatattct
     6301 tgaagggaca gcttctttgg tatgaactca gccagtgagc tgtgtccatt tcctccataa
     6361 ttctggatat cacctatctt tttagcacca agtatttatt ctcactagca gtagacagac
     6421 ttaaccagtc tctaccacga cccagtagac tctgacaaca gactcttcct ttaaggtgtc
     6481 aacaactgac ttactaggca ggactgccat ccttagcatc agcccagttt tggttaccag
     6541 ggactatatc ttttgttcca ggagagagta gaaagaaaag gagagatccc atccttgggt
     6601 catagaccca ttttcattgc cattatctgt aagtatgtgc actgcatgga ggacagccct
     6661 aatagtctcc cctctgctgc cactgcctga ttctccactc tgtccccact tgccagtgaa
     6721 gtttggattt atcagccact tgtggcccat tacagagctg acccaaggtg gtgtcaccaa
     6781 tgcctcagca gggtggagtg cagttcagag cttataaata gctggcaggg catgaggaga
     6841 tgattttctt gcatgtgcaa ttctctaggg gccatcagga aacagagttt aaagtaccct
     6901 ttgattactg tcagaactgt cagcatttgc agttttcccc gagcagtaat tggccagctc
     6961 ccttctatca gtagccagtg ttaactagtt taccaaatgc tgtaaacaat tgccctgaat
     7021 gaaatagctg tcagtggagc tctaagatct cctttagagg gcacagtgag gtgggctgtt
     7081 gggatggccg taggcagggg agaggaagaa ggccactgat gagactgctg acctcctcag
     7141 aaggttgacc tcttctagag agactgcctc ccagtgttta agtggctcat tgctccttct
     7201 gactctgtaa aatggctctg gaggacagct cacaggaatt ctgattgctg aggtgggtga
     7261 ttgagagctg ttcaccatgt gtgcagcctg tgctcccctt ctatggatat gtgtgcaatt
     7321 tttgtatgta tttttttagc tgtatattac agtgtttatg ttgcaacttc acctgaagcg
     7381 agtctacaga aaccctcccc cttcttccct tggcacatat ttgcgctaag agatgagcgt
     7441 tccttccact ggggttgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7501 atgtatacac atgttcacct gtgcttgttt tattcaaaac tgtgagtatg tgtttacttt
     7561 gaccttgaaa tcccaagagt cactcgaaca atgtgtgtgt acacttgtgt gcccatgtgt
     7621 gcgtgcacac gcatgctgga agtagaggcc aggcctctag aaaagcaacc tgtggcaggg
     7681 ttgacagaca tgttctctgt gcctagtgag tgagtgagcc ctgaggcccc tcatctacca
     7741 gtgcagagtt tccttaaagc caagactgac tgtgcagtat tacttacata gcacattagt
     7801 ggccttggaa atttctaaac ttttaacatt tttaacagct tttcatgtga ttcatgtata
     7861 acaaagagga tcaggaaagg aaaataatgt attttattgt gctcttatta cttttttaag
     7921 tgattgtaat atgtgaggct ctatttggag aatgtccctt atcccctggc cagcaatata
     7981 ggactcacag gaaagaaggc tggttagccc acaaagagtc ttgcctcctc tagccagagt
     8041 gcagggctct gctcctagga atggccatgg agtgggcatg ctgctgcagg ccagcagtct
     8101 gctcttggtg tcctgtggca tggccgcgcc ttgccttgaa ggttggtgtt tggttcatct
     8161 tagcatagaa catgtgcaca taactccttg cctgattatg cactgaaggg cagcacacag
     8221 accactgacc atgtcagcca gcacattccc ttaacctccc ccacctccca tcttgcaatg
     8281 tcttgcatat tgagatatac cttctcaatg tcatgttcgt ggtgccttgc ccatgtaaat
     8341 ttcaatgttg actatttaat gaggttacat atttaatgct gaatgtttcc tggtccctct
     8401 atttgttatg tctctactta tctttttttt tctttctctt tttctgttat gatttggatc
     8461 cagtcttcac tttaactcta tggtataata agcatggaca cctccactac cattatctta
     8521 cagcactggt aaaccaagca cctcttcata tcagatgttt ttagtactgt ttttatattc
     8581 agatttgtta agggcaaaag atgaatgcaa actagccaga gaaaagcctc aggcttccca
     8641 gagacaggtg gctagattaa tttttctctg ggtgatggcc aaagaatgtg agatagagag
     8701 ggaagggggt gttacaggtt agagcagaat agagaaagaa actatatttc taggcaaaaa
     8761 gtactacatg agttggtctt cagaatattt ttccaaaaaa atgaaattca gtcagtttaa
     8821 cattatcaca aatatttttc attttaattg tggtaggacc tttgaatttg ttcatcaact
     8881 ggaaagaaat cctaccctct gtagacacat acacacacat ttatatatct atgtgtttga
     8941 attgtgacta gtatagcact ttacattttc tcaagtaaca ggaagttatt gccttcaata
     9001 ttgattggaa attgttttct attgaagtct atactgtaga gttttccatt tatattgaga
     9061 gtttgaacaa ttaagctcct attggaactt ttccttaagc cacatgagca tgaacaatct
     9121 ctgtgacact tgggtgctag agaaacacaa cagtggctaa agtagccaag agcaagactg
     9181 aatgtatagc agagtgcctt gttagagtgg taacctagtc ttggaagcaa ttcagtggat
     9241 gtttcataaa gaggactaga acttcaagaa gcctcctgtg ctggctgagg tgagcccttt
     9301 tggtatatgt acactactgg gttgcctaga agccaattca tgaagaataa cagcaggatg
     9361 agaaaaagag tcactgtaac ctgtggaaca acaacgtaag aatcaccaac ccctaggctg
     9421 agctagaact ggaagccagg aatatggcaa tgagtttcaa gatttgccct cagaagatga
     9481 actgggtgag gtatgggttg aggtgagggc acaagcactc atcctaccta ccctgctgct
     9541 gttgaggagc tagaagtaaa acatattggt taggatttta acagttggag tagcctccca
     9601 ttatcagatt aagtgggggt cacttttgct tccaaactca gaataagttt gtaaacatgg
     9661 gaaagtctaa gccaagagat ctaaagctct agaaatatta atgacatttc tgggagtaaa
     9721 actactaaaa attatatctt aaaagtttct agtagtatga agttcccagg ccaggcgcag
     9781 tggctcacgc ctgtaatccc aacactttag gagggcgagg tgggcagatc acctgaggtc
     9841 aggagtttga gaccagcctg gccaacatgg tgaaactcca tctctactaa aaatacaaaa
     9901 attagccggg catgatggca ggcacctgta atctaagcta ctcgggaggc tgaggcaaga
     9961 gaatctcttg aacccaggag gcaaaggttg cagtgagcca agatcacgcc atcgcactcc
    10021 agcctgggag attgagcaac ttcgtctcaa aaaaaagttt ccaggaaaga tctttgggtc
    10081 ctactaagcg tctcaatttt gagttatgaa tactttggtt cagggaagta acctgacatt
    10141 tggttttgct ggaattaggg ttttcgctag atttgactgc aattaggatt tcttctagat
    10201 tttaccagca gtgatctgaa aaagtacagg ctagtcacag aaatagagat ggatttagga
    10261 tactacacta tatttcaaat gattctagat gtgtaaatat agtatgaaca ttcttggaga
    10321 ggggtgagac aaactggact tgctgggcca accctggcat gtttttcatt ctcctcacta
    10381 gttgacaagg attgaaaatt ccgctccagc ctaatgagtg aacaggatgg ctgcccacaa
    10441 aaccaagtta ttagctttat gtcatcgaag ctcatattct caaaggccca aagtgctgtg
    10501 ccgagacaaa tccaagtgct gctcttgctg aagtgcgcac tgaagtccag ctgtccgtgc
    10561 ttacagcagt gccaaaagtg gggattccat ggaccaaaaa cggtgccata agggaagcca
    10621 gaagagttgc agtgcaagat cctgggagag attataccaa agaaatcaag cctttttatc
    10681 ctgatgcttc taagatgatg agggtgagct gtgacagatg ctgtgggagg ggagttcaca
    10741 tacattccat gtcaaggcat ttagctgata aatccatcct cagaaaagca cctttttaaa
    10801 aagaagtcca ttttgtatat tagaagatga tgatgatgat gaagatgaag aaagccctgg
    10861 cagatacaaa gttcctcagt atttaaggag aaggatctct tccatgtccc cttgacatga
    10921 ctgtggctat tgcccaaacc tcagcttgag aacatcatat aatactgaga gcggaagatt
    10981 tttttaaaga gatatggtag tttaaatagg tttgtttaaa gaactcttgg gaaatttctt
    11041 aacaaattgt aattgttttt ataaaccttc ctacagagtt gtgtaccatt catcatcacc
    11101 atcatttaat atctgtaagt gcccagaagg tacaaggtgc tatacagccg ttaaacaccc
    11161 cctccctgca actgtttaaa attagaatag atttttgcat caaccactgc tagaagcttt
    11221 ccccacctag gtgggctgca agggcctgca ttcccaacat cccattacat gtttcccact
    11281 ccagaaagtc tctgggttta ttccagcttt ttatcttgac gtgtcccatt cacccccatg
    11341 gtttggtttg gtttggtttt gttttttaaa tcaagaaagt ttcagggaat caaaagaggt
    11401 cggacatttt ttaacctagc agaagtacag agtcatatat tttgctacga atattgttgt
    11461 agtacagaaa acccactttg cccgttagtg tgtggatagt atgtgtgtgt ccgcactcat
    11521 gataacttga atgttgaacc agacataggg ttcatttttg aaaggttaaa ccacactgtt
    11581 tcaggaactt gctccaaata ctacttggtt atcccttcct ttaccagtta gaactaaaga
    11641 gtgtgatgta tgaacacact gggttgggat tttctgttga ggatatgcag ggcattttgg
    11701 catgaggcaa atacagaagc aagatttcat tctacttggt gatttgaatc atgacagtcc
    11761 tcattccaat ctctctttaa ttctctctgg ccctgcccac actctgtatt tgaaaatctt
    11821 gtttttgctc tttccggagc ttcacccctc tacttacata ttgtaaagtt gtataaatct
    11881 atcattgaaa ggtcctctct gccagcagtg gtgccaccct ttggtttgct gtggtacttt
    11941 gctgtgtact ccgtggcatc atgttactgt gtaaataaat tcattttaat acacactaaa
    12001 aaa
//
