LOCUS       NM_173791               9672 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens PDZ domain containing 8 (PDZD8), mRNA.
ACCESSION   NM_173791
VERSION     NM_173791.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Al-Amri,A.H., Armstrong,P., Amici,M., Ligneul,C., Rouse,J.,
            El-Asrag,M.E., Pantiru,A., Vancollie,V.E., Ng,H.W.Y., Ogbeta,J.A.,
            Goodchild,K., Ellegood,J., Lelliott,C.J., Mullins,J.G.L.,
            Bretman,A., Al-Ali,R., Beetz,C., Al-Gazali,L., Al Shamsi,A.,
            Lerch,J.P., Mellor,J.R., Al Sayegh,A., Ali,M., Inglehearn,C.F. and
            Clapcote,S.J.
  TITLE     PDZD8 Disruption Causes Cognitive Impairment in Humans, Mice, and
            Fruit Flies
  JOURNAL   Biol Psychiatry 92 (4), 323-334 (2022)
   PUBMED   35227461
  REMARK    GeneRIF: PDZD8 Disruption Causes Cognitive Impairment in Humans,
            Mice, and Fruit Flies.
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Walch,P., Selkrig,J., Knodler,L.A., Rettel,M., Stein,F.,
            Fernandez,K., Vieitez,C., Potel,C.M., Scholzen,K., Geyer,M.,
            Rottner,K., Steele-Mortimer,O., Savitski,M.M., Holden,D.W. and
            Typas,A.
  TITLE     Global mapping of Salmonella enterica-host protein-protein
            interactions during infection
  JOURNAL   Cell Host Microbe 29 (8), 1316-1332 (2021)
   PUBMED   34237247
REFERENCE   3  (bases 1 to 9672)
  AUTHORS   Shirane,M., Wada,M., Morita,K., Hayashi,N., Kunimatsu,R.,
            Matsumoto,Y., Matsuzaki,F., Nakatsumi,H., Ohta,K., Tamura,Y. and
            Nakayama,K.I.
  TITLE     Protrudin and PDZD8 contribute to neuronal integrity by promoting
            lipid extraction required for endosome maturation
  JOURNAL   Nat Commun 11 (1), 4576 (2020)
   PUBMED   32917905
  REMARK    GeneRIF: Protrudin and PDZD8 contribute to neuronal integrity by
            promoting lipid extraction required for endosome maturation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9672)
  AUTHORS   Guillen-Samander,A., Bian,X. and De Camilli,P.
  TITLE     PDZD8 mediates a Rab7-dependent interaction of the ER with late
            endosomes and lysosomes
  JOURNAL   Proc Natl Acad Sci U S A 116 (45), 22619-22623 (2019)
   PUBMED   31636202
  REMARK    GeneRIF: PDZD8 tethers the endoplasmic reticulum to late endosomes
            and lysosomes via an interaction with Rab7.
REFERENCE   5  (bases 1 to 9672)
  AUTHORS   Hirabayashi,Y., Kwon,S.K., Paek,H., Pernice,W.M., Paul,M.A.,
            Lee,J., Erfani,P., Raczkowski,A., Petrey,D.S., Pon,L.A. and
            Polleux,F.
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in
            mammalian neurons
  JOURNAL   Science 358 (6363), 623-630 (2017)
   PUBMED   29097544
REFERENCE   6  (bases 1 to 9672)
  AUTHORS   Bai,S.W., Herrera-Abreu,M.T., Rohn,J.L., Racine,V., Tajadura,V.,
            Suryavanshi,N., Bechtel,S., Wiemann,S., Baum,B. and Ridley,A.J.
  TITLE     Identification and characterization of a set of conserved and new
            regulators of cytoskeletal organization, cell morphology and
            migration
  JOURNAL   BMC Biol 9, 54 (2011)
   PUBMED   21834987
  REMARK    Erratum:[BMC Biol. 2024 Oct 10;22(1):228. doi:
            10.1186/s12915-024-02036-3.. PMID: 39390519]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9672)
  AUTHORS   Henning,M.S., Stiedl,P., Barry,D.S., McMahon,R., Morham,S.G.,
            Walsh,D. and Naghavi,M.H.
  TITLE     PDZD8 is a novel moesin-interacting cytoskeletal regulatory protein
            that suppresses infection by herpes simplex virus type 1
  JOURNAL   Virology 415 (2), 114-121 (2011)
   PUBMED   21549406
  REMARK    GeneRIF: PDZD8 is a novel moesin-interacting protein that
            suppresses infection by herpes simplex virus type 1.
REFERENCE   8  (bases 1 to 9672)
  AUTHORS   Henning,M.S., Morham,S.G., Goff,S.P. and Naghavi,M.H.
  TITLE     PDZD8 is a novel Gag-interacting factor that promotes retroviral
            infection
  JOURNAL   J Virol 84 (17), 8990-8995 (2010)
   PUBMED   20573829
  REMARK    GeneRIF: A mutant of PDZD8 lacking the coiled-coil domain in its
            Gag-interacting region failed to bind Gag and promote HIV-1
            infection.
REFERENCE   9  (bases 1 to 9672)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   10 (bases 1 to 9672)
  AUTHORS   Lee,S.Y., Obata,Y., Yoshida,M., Stockert,E., Williamson,B.,
            Jungbluth,A.A., Chen,Y.T., Old,L.J. and Scanlan,M.J.
  TITLE     Immunomic analysis of human sarcoma
  JOURNAL   Proc Natl Acad Sci U S A 100 (5), 2651-2656 (2003)
   PUBMED   12601173
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL359836.16 and AL391988.14.
            
            On Nov 23, 2018 this sequence version replaced NM_173791.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK091746.1, SRR1803616.264173.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334464.7/ ENSP00000334642.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1085              AL359836.16        29261-30345         c
            1086-1208           AL391988.14        98596-98718         c
            1209-1311           AL391988.14        76488-76590         c
            1312-1474           AL391988.14        47802-47964         c
            1475-9672           AL391988.14        34890-43087         c
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.3-q26.11"
     gene            1..9672
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="PDZ domain containing 8"
                     /db_xref="GeneID:118987"
                     /db_xref="HGNC:HGNC:26974"
                     /db_xref="MIM:614235"
     exon            1..1085
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..3678
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="sarcoma antigen NY-SAR-84/NY-SAR-104"
                     /codon_start=1
                     /product="PDZ domain-containing protein 8"
                     /protein_id="NP_776152.1"
                     /db_xref="CCDS:CCDS7600.1"
                     /db_xref="GeneID:118987"
                     /db_xref="HGNC:HGNC:26974"
                     /db_xref="MIM:614235"
                     /translation="MGLLLMILASAVLGSFLTLLAQFFLLYRRQPEPPADEAARAGEG
                     FRYIKPVPGLLLREYLYGGGRDEEPSGAAPEGGATPTAAPETPAPPTRETCYFLNATI
                     LFLFRELRDTALTRRWVTKKIKVEFEELLQTKTAGRLLEGLSLRDVFLGETVPFIKTI
                     RLVRPVVPSATGEPDGPEGEALPAACPEELAFEAEVEYNGGFHLAIDVDLVFGKSAYL
                     FVKLSRVVGRLRLVFTRVPFTHWFFSFVEDPLIDFEVRSQFEGRPMPQLTSIIVNQLK
                     KIIKRKHTLPNYKIRFKPFFPYQTLQGFEEDEEHIHIQQWALTEGRLKVTLLECSRLL
                     IFGSYDREANVHCTLELSSSVWEEKQRSSIKTVELIKGNLQSVGLTLRLVQSTDGYAG
                     HVIIETVAPNSPAAIADLQRGDRLIAIGGVKITSTLQVLKLIKQAGDRVLVYYERPVG
                     QSNQGAVLQDNFGQLEENFLSSSCQSGYEEEAAGLTVDTESRELDSEFEDLASDVRAQ
                     NEFKDEAQSLSHSPKRVPTTLSIKPLGAISPVLNRKLAVGSHPLPPKIQSKDGNKPPP
                     LKTSEITDPAQVSKPTQGSAFKPPVPPRPQAKVPLPSADAPNQAEPDVLVEKPEKVVP
                     PPLVDKSAEKQAKNVDAIDDAAAPKQFLAKQEVAKDVTSETSCPTKDSSDDRQTWESS
                     EILYRNKLGKWTRTRASCLFDIEACHRYLNIALWCRDPFKLGGLICLGHVSLKLEDVA
                     LGCLATSNTEYLSKLRLEAPSPKAIVTRTALRNLSMQKGFNDKFCYGDITIHFKYLKE
                     GESDHHVVTNVEKEKEPHLVEEVSVLPKEEQFVGQMGLTENKHSFQDTQFQNPTWCDY
                     CKKKVWTKAASQCMFCAYVCHKKCQEKCLAETSVCGATDRRIDRTLKNLRLEGQETLL
                     GLPPRVDAEASKSVNKTTGLTRHIINTSSRLLNLRQVSKTRLSEPGTDLVEPSPKHTP
                     NTSDNEGSDTEVCGPNSPSKRGNSTGIKLVRKEGGLDDSVFIAVKEIGRDLYRGLPTE
                     ERIQKLEFMLDKLQNEIDQELEHNNSLVREEKETTDTRKKSLLSAALAKSGERLQALT
                     LLMIHYRAGIEDIETLESLSLDQHSKKISKYTDDTEEDLDNEISQLIDSQPFSSISDD
                     LFGPSESV"
     exon            1086..1208
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1311
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1474
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..9672
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4620..4625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="hexamer: AATAAA"
     polyA_site      4640
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="major polyA site"
     regulatory      6431..6436
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="hexamer: AATAAA"
     polyA_site      6454
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="major polyA site"
     regulatory      9459..9464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="hexamer: AATAAA"
     polyA_site      9486
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="major polyA site"
     regulatory      9646..9651
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
                     /note="hexamer: ATTAAA"
     polyA_site      9672
                     /gene="PDZD8"
                     /gene_synonym="IDDADF; PDZK8"
ORIGIN      
        1 acaccggccc ttcgggagcg tctgaggagg cgcgggccgc tgcgggctcg ggcgccgcag
       61 cgcgccggcc cgagcccctg gacgaggccc acggagccgc tcgccccgac ccagccgccc
      121 gatgtcctca aaatggaggc agcgggggcg gcggcgtgaa gaaagcggcg ctgtgggcgc
      181 gggagtaggg gcccgggcgg aggcggtggc gggatggggc tgctgctcat gatcctggcg
      241 tcggccgtgc tgggttcctt cctcacgctc ctcgcccagt tcttcctgct gtaccgcaga
      301 cagcccgagc cgccggcgga cgaggccgcc cgcgcgggcg agggcttccg ctacatcaag
      361 ccagtgccgg gcctgctcct aagggagtac ctttatggcg gcggccggga tgaggagccc
      421 tccggagcgg cccctgaggg cggcgcgacc cccaccgcgg cccccgagac ccccgccccg
      481 ccgacgcggg agacttgcta cttcctcaac gccaccatcc tattcctgtt ccgggagttg
      541 cgggacaccg cgctgacccg ccgctgggtc accaagaaga tcaaggtgga gttcgaggag
      601 ctgctgcaga ccaagacggc cgggcgcctg ctggaggggc tgagcctgcg ggacgtgttc
      661 ctgggcgaga cggtgccctt catcaagacc atccggctcg tgcggccagt cgtgccctcg
      721 gccaccgggg agcccgatgg ccctgaaggg gaggcgctgc ccgccgcctg ccccgaggag
      781 ctggccttcg aggcggaggt ggagtacaac gggggcttcc acctggccat cgacgtggac
      841 ctggtcttcg gcaagtccgc ctacttgttt gtcaagctgt cccgcgtggt gggaaggctg
      901 cgcttggtct ttacgcgcgt gcccttcacc cactggttct tctccttcgt ggaagacccg
      961 ctgatcgact tcgaggtgcg ctcccagttt gaagggcggc ccatgcccca gctcacctcc
     1021 atcatcgtca accagctcaa gaagatcatc aagcgcaagc acaccctacc gaattacaag
     1081 atcaggttta agccgttttt tccataccag accttgcaag gatttgaaga agatgaagag
     1141 catatccata tacaacaatg ggcacttact gaaggccgtc ttaaagttac gttgttagaa
     1201 tgtagcaggt tactcatttt tggatcctat gacagagagg caaatgttca ttgcacactt
     1261 gagttaagca gtagtgtttg ggaagaaaaa cagaggagtt ctattaagac ggttgaatta
     1321 ataaaaggaa atttacaaag tgttggactt acacttcgtc ttgtccagtc aactgatggg
     1381 tatgctgggc acgtcatcat tgaaactgtg gctccaaact cgcctgctgc aattgcagat
     1441 cttcagcggg gagatcgact tatcgccatt ggaggtgtga aaatcacatc aacactgcaa
     1501 gtgttgaagc ttatcaagca ggctggtgac cgagtcctgg tgtactatga aaggcctgtt
     1561 ggccagagta atcaaggtgc agtgctgcaa gataactttg gccagttgga agaaaacttt
     1621 ttgtcaagct catgccaatc gggttatgaa gaggaagctg ccgggttgac agtagatact
     1681 gaaagtagag agctggattc tgaatttgaa gacttggcaa gtgatgtcag agcacaaaat
     1741 gagttcaaag atgaggcaca atcattaagt catagtccca aacgtgttcc aacaacactt
     1801 tctattaaac cccttggagc tatatcacca gttttaaacc gtaaattagc tgtaggaagt
     1861 cacccactac caccgaaaat tcagtccaaa gatggaaata aacctccacc cctaaaaact
     1921 tctgagataa cagacccagc acaagtgtca aaaccaaccc aaggatctgc tttcaaacca
     1981 cctgtgccac cacgaccaca agcgaaagtt cctttgcctt ccgccgatgc tccaaatcag
     2041 gcagaaccag atgttctcgt tgaaaagcca gagaaggtgg tgccacctcc tcttgtagat
     2101 aaatctgctg aaaagcaagc aaaaaatgtg gatgccatag acgatgcagc tgcacctaag
     2161 caatttttag caaagcaaga agtggccaaa gatgtcactt cagaaacttc ctgccctact
     2221 aaggacagtt cggacgaccg tcaaacatgg gaatcatcag aaattcttta tcgtaataag
     2281 ctaggaaaat ggacaagaac cagagcatcc tgtttgtttg acatagaagc ctgtcacagg
     2341 tacttaaaca ttgcattgtg gtgcagggat cctttcaagt tgggaggtct catctgtttg
     2401 gggcatgtta gtttaaaact tgaagatgtg gctttaggat gcctagctac atcaaacacg
     2461 gaataccttt ccaaattgag actggaagcc ccctcaccta aggctatagt cactagaacc
     2521 gcactacgca atctgagtat gcaaaaggga ttcaatgaca aattttgcta tggtgacatt
     2581 actattcact tcaaatattt gaaagaagga gaatcagacc accatgtagt tactaacgta
     2641 gaaaaagaaa aagaacccca tttggttgaa gaagtttctg ttctccctaa agaggagcaa
     2701 tttgttggac agatgggttt aacagaaaac aaacacagtt ttcaggatac tcagttccag
     2761 aacccaacat ggtgtgacta ctgtaagaaa aaagtttgga ctaaagcagc ttcccagtgt
     2821 atgttttgtg cttatgtttg ccataaaaaa tgtcaagaaa agtgtctagc tgagacttct
     2881 gtttgtggag caactgatag gcgaatagac aggacactga aaaaccttag gctggaagga
     2941 caggaaaccc tcttaggcct gcctcctcgt gttgatgctg aagctagcaa gtcagtcaat
     3001 aaaacaacag gtttgacaag gcatattatc aatactagtt ctcgtttatt aaatttgcgt
     3061 caagtctcta aaactcgcct ttctgaacca ggaaccgatc tcgtagaacc ttcaccaaaa
     3121 cacacaccca acacgtcaga caacgaaggc agtgacacgg aggtctgtgg tccaaacagt
     3181 ccttctaaac ggggaaacag cacaggaata aagttagtga gaaaagaggg tggtctggat
     3241 gacagtgttt tcattgcagt taaagaaatt ggtcgtgatc tgtacagggg cttgcctaca
     3301 gaggaaagga tccagaaact agagttcatg ttggataagc tacagaatga aattgatcag
     3361 gagttggaac acaataattc ccttgttaga gaagaaaaag agacaactga tacaaggaaa
     3421 aaatcacttc tttctgctgc cttagctaaa tcaggtgaaa ggctacaagc tctaacactt
     3481 cttatgattc actacagagc aggcattgaa gatatagaaa ctttagaaag tctgtcttta
     3541 gaccagcact ccaaaaaaat aagcaagtac acagatgata cagaagaaga ccttgataat
     3601 gaaataagcc aactaataga ctctcagcca ttcagcagca tatcagatga cttatttggc
     3661 ccatccgagt ctgtgtagca gacaggtcta tttaagcttt caaatgaaca gggtaaagtt
     3721 gcatctaaag taccacagat acaaccatgt ttaaatcctc gtatgcactc tggcctgctt
     3781 ctccagttac ttgcttgtgt aagaacaaaa atgagaaagg ttgttttcca gtaaaaacat
     3841 gaccagctta ctaattggtt gttttggatt gcatttatag ctatgctttt ttgggtttat
     3901 actgggaatt tatttttact aaattattta acttttctaa ttatgtaatt atgtaagcta
     3961 gcttttcatg tttatgtatg tatggtgtcc ccttgtgtta tttttcttcc tcttggtttt
     4021 tgaattagtg ttaaatagaa tactgtctag attcttaaaa tattttcatt tccatcatgg
     4081 ttataacaaa tttgctgcat gcccaaactg acaacagcaa tcactgaggg aacaggtttt
     4141 gaatctttct tttgtgttat gaagtttatc gtctctactt gcttgagatt tttgttattt
     4201 tgggggtttg ggggtgcttt ttgttttgtt tttgccaaat gtaacatgaa agcagatgct
     4261 gcagctttag tctgttatgc tgatttagta aaaaaaaatt ttttacatat attgcttgct
     4321 ttcgatgctt ctgtgaaatt tttttctaaa gcttttgtgc agctgtatgg taaaaatatg
     4381 gtgattaatt tgaagagctt acattgaaag acaatgtaat aggaaataaa tgtagattgc
     4441 agttggtcaa gaattttgta gagaggataa caagacttaa ttactgaaaa acagtaacat
     4501 agcattttga aatataatct tttaaaatat tgatgctttc cttttaaatg gaaatttaaa
     4561 ttttataatt aaaagtttaa acatttatga taattttcct catcagttct cccataggaa
     4621 ataaagcatg tgaaagggta tttaaagttt tggaggactc tttttaaaat gactgtgttg
     4681 ataactagtt tgggctggtt ttgttttaga aaaaacattt tcatgtagga gtattctgtg
     4741 aaggaaagga atcatgcaaa atatactttt tgctttggcg tcttacagtt gtaaaggaat
     4801 ggtgatcatt ctgaatactt ctgtagtgag tattcattaa gaaaagaatg acaaaaatag
     4861 tattcataca taggaattgg gaccagttct ctgaaaatga agatatactc acactgggtc
     4921 cattttacgg gtcccagtaa catttgactg agggaaaaca agtaagctcg actgttgttg
     4981 gggtacctta atggtttcgg tctgttatat acctataaaa tagacaagta tatgtttgtc
     5041 cctttgtagg taactgctat gatgattgct atatagtgaa actgacatga gtttaaaact
     5101 agatggagca gcttttattg ctggcaggaa aaagcatggt gtaatattaa ggactggggc
     5161 ccaggtctag actccaggac tccataaaag agtttaagtt caagcccttc cattaatgaa
     5221 ctgtgtaagc ataggcagtt acaaaaggac ccatctctaa aatgggaata ataatacttt
     5281 acctccctca cagtattgtt gtgagggaaa caatagcata actgcaaaag tgctttgaac
     5341 agtaaaaaga tatacaagta aaaggcatta tttatatgag taagaaagac atttttgagg
     5401 ggagaaaagt tattccactt taaaaatcaa tagccaagtc tcatgccttt tgttacttta
     5461 gtaagcgaaa atttcaggta ctaaatacct ttcttagcca agtttataac aaaaacatct
     5521 cagtatactt cattacttat ttaagcgtga tgtctttttt tttttttttt tttttttttt
     5581 tttttttttt ttttgagaca gagtctcgct gtctcccagg ctggagtgca gtggcatgat
     5641 ctcggctcac tgcaagctcc gcctcccagg ttcacgccat tctcctgcct cagcctccgg
     5701 agtagctggg agtacaggcg cggctaattt tttgtatttt tagtagagac ggggtttcac
     5761 catgttagcc aggatggtct cgatctcctg acctcgtgat ccacctgcct cggcctccca
     5821 aagtgctggg attacaggcg tgagccacca cgcccggcca cgtgatctta ttttaaaaga
     5881 acactgctat agaagaattt acacaccaaa ttccctgaaa gctaaagtat tgttccataa
     5941 cttacacttg aggcaatctg ctcagtgtgt aaccacttta taaaaaataa cttaccattg
     6001 ataacctgga aggtggacta cttgtaagta gctggtgttt attgattaaa ccatggaagt
     6061 tttcatgata cccaagcatg ttgctgaaac tgatgcattt gaaagagctg ttgaaataat
     6121 gcaaaacttt ggttttataa gaatctactc atgttttaaa agtatttaac tctgtagacc
     6181 acaattatag aaagctcagt aatgctgcta atgacatttg taaactatat ctgtaatgta
     6241 gtgcaatatt tttatatttc actgggagag gcagtagaga catcatagtt gtactttagt
     6301 gcaagtgggg gaacactcca aatagcctag agtatagtaa ggtacaaagc ttcaatgaaa
     6361 aaagctgtat tatattacat tactgatgta atcttgctgc catgctatgt atcaatagct
     6421 aaaaaataat aataaagact accacagaaa acaagaatat cttcatttaa tgaaatcttt
     6481 aaaaaaggtg attgctgcat ctctatggaa ttatcttctg gtataatttg gccatgcaga
     6541 aaagcataac aaagtccact tttcctaaac aaaattgctg ttttgcagac tgccccaaaa
     6601 cattttttta ctattgtttc cttatggatt atcaagttta tgggtttcct ttggctatgt
     6661 attagtaatt agactttaaa atatattttc tacattagta ttagctgagt gaatggctta
     6721 gacaaactga gcaacatttt agcattatta gcaatcataa taggctatac ttcttcatat
     6781 caagccttat attcaatatc agaagtactt gaaataagtg gctattttgt gatgctggga
     6841 ggagaaaaca caacctaaaa attttgaggg gtactatcaa gttctaaatg tatagctgaa
     6901 ttttttttaa agcaatttgg aaatatttgg ctcttaaaaa taagataaat aataaaatgt
     6961 taagtcccct aaaagtgaat aaattttaaa tacctacttt taaaaatact gtcttctaaa
     7021 ttgacataat tgcttttctt accaaaagaa ggagaggttc ccctaattcc ttttgggcca
     7081 tagatccgct tttaggatct gattaaagat gtggactttc accgcaggaa gatacccatg
     7141 ggcacaccat tcaaaatgta tcagatttca acggttttac aaactccccc gggattaagt
     7201 caaatgggtg gatattacag ctgttttcat cagatatggt tatttgttga caaccatcag
     7261 gacaacaatg tttatagatg gaaggataac ttcctggttt ttttccacat tgaatgtggc
     7321 tagttacata tctcaattta aaataaattg tggaaagcca aaaaagtatg gtcaagctaa
     7381 cctggggtgg cttttacctg atgcctacaa gcacagaaaa atagttttta attaaatatc
     7441 cacagtcaaa atgatacctt atgtatttta ttccaacaac atttggttta taaaggaata
     7501 caaacaggca caaaacatgg ttcagaagat ttattaagta aacttgctaa aatatggaca
     7561 gatacactta gcagtcaaac agttgaatat tcattgctac ctcattaaag tttttgtatc
     7621 tgtattacca ggtccaaaca taaaaaccac ctctgttcaa aaaataaatg ttcagagagc
     7681 tgtatgttct ttgttctggt atgtacattt taaaaaaaca cctctttcca gtcttgctaa
     7741 ccaagaatat tagtcatata aaagaactta gaattttttt ccccaagtac aagctatctt
     7801 ttgctccaaa acagttctga aggttttatt tatattttat cttatcccga gggaccaaca
     7861 gcaggcatac ctttgccagg ccttcttgca gaaagacaca gagccgtaaa ggcaaaaata
     7921 aaattgcaat aaagtatatg gtattggggg cagggagaac cagaaaccct caaagagaac
     7981 caatttgtag cacgttcttt tttaaggctc tacccctgta gaagtaagaa actagcctgc
     8041 ctttttagcc atatgagagt ttcctccaga gccatcttcc aaagtggcag acttggccaa
     8101 gttgcccaat gccaaataag tgagttggaa gttcgtttgc ttcaaacaca ctgcacttag
     8161 aaaccagact tgaaataatc gaagccccac agaaaagctt catgaaacga agtgttactt
     8221 tcctagagaa taagaaagtc acaagattga ggagtctgtt ctaaagttct ttgctccttg
     8281 atgtcgaagt taacacaaac acaatcttag cttaacgaaa ctaagtaatt tttgtttatg
     8341 attatagaac tgcaggattc acaagtagta ttagagacat tccacactgc acactccagt
     8401 catatctctt aacaaaatga cataaattga caatgttcac taacagtatt tgctgttaca
     8461 ctgaaaaggc acatcaatgt tgtcatgctt agaaaaatac acaacactgc acactggatg
     8521 atatagttag cacatacatt tttagatgtt ccatttttct tttctgcctg atcactatta
     8581 tttttacttc ttaatatatg attcaagaca taatttcatt cttcattttc atttaaaatt
     8641 tgtcttcaca ttgatgtgaa caactttcag gagtctaaaa aatagtagtt tcagacaacc
     8701 taaaataaat atattcctag aaaacacttg gatgacctgc aaacagatca aagatatagt
     8761 cagatattaa agtcagagca gctgttttca gcttcttgtt tccttttcta aaacttgaag
     8821 tagatggcga ttaaccttat catgtttaaa caaccttgac agtataaggt acatgtaccc
     8881 tcaattttct tcatccagta agacaagtat gcatcttaaa gggaaagctt ttagttcata
     8941 acaatatgtt tggttgcttt tctctgcttt cggaacattt actgtttttc ctttattggc
     9001 tttagtgttt gctgccaacc agataacatg ttaacatata atttacaact ctctctaggt
     9061 aggtgattac atagaaaact aatgccagct aagatttatg gattcaaaag ataactgaca
     9121 caagttcttg tcctctacac ttgagagaac tagctgttta acgcataaat atattgatag
     9181 taactacaag ttaaataaca ttcagtgtca ctatataaca tgctgtctta cttgattctg
     9241 tttttcagac ttcattctag tttctctttg ttggaagagt ctcagagata atgtgtgctt
     9301 taattttaaa cactcattac caatatgatt tttgcccaaa tgtttgttaa tgtaagttaa
     9361 aaaattatat ttggtttgga ttcaaatatt tatagattta cacaatacct atttcatcaa
     9421 aatatattgt atgatattta ataaaattaa atacataaaa taaagtttca acactattta
     9481 tacaaacccc tagaaaagct tctttttctt tataaggctg ttggcaatcg accataacca
     9541 tggaaaggaa ataataacct ttggttttac tctttcacca gggatggatg gtatgactaa
     9601 gacagttctg gatgagttgt gtcactggaa acactgtttt atacaattaa acactttgat
     9661 gatttttgag ga
//
