LOCUS       NM_020315               2012 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens pyridoxal phosphatase (PDXP), mRNA.
ACCESSION   NM_020315
VERSION     NM_020315.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Schulze,M., Hutterer,M., Sabo,A., Hoja,S., Lorenz,J.,
            Rothhammer-Hampl,T., Herold-Mende,C., Flossbach,L., Monoranu,C. and
            Riemenschneider,M.J.
  TITLE     Chronophin regulates active vitamin B6 levels and transcriptomic
            features of glioblastoma cell lines cultured under non-adherent,
            serum-free conditions
  JOURNAL   BMC Cancer 18 (1), 524 (2018)
   PUBMED   29724193
  REMARK    GeneRIF: In non-adherent glioblastoma cell lines cultured in
            serum-free medium, chronophin knockdown induces phenotypic changes,
            e.g. in colony formation and transcription, but these are highly
            dependent on the cellular background. CIN knockdown in the
            glioblastoma cell lines NCH421k and NCH644 increased active vitamin
            B6 levels.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Delorme-Walker,V., Seo,J.Y., Gohla,A., Fowler,B., Bohl,B. and
            DerMardirossian,C.
  TITLE     Chronophin coordinates cell leading edge dynamics by controlling
            active cofilin levels
  JOURNAL   Proc Natl Acad Sci U S A 112 (37), E5150-E5159 (2015)
   PUBMED   26324884
  REMARK    GeneRIF: Chronophin has a role in coordinating cell leading edge
            dynamics by controlling active cofilin levels
REFERENCE   3  (bases 1 to 2012)
  AUTHORS   Huang,T.Y., Michael,S., Xu,T., Sarkeshik,A., Moresco,J.J.,
            Yates,J.R. 3rd, Masliah,E., Bokoch,G.M. and DerMardirossian,C.
  TITLE     A novel Rac1 GAP splice variant relays poly-Ub accumulation signals
            to mediate Rac1 inactivation
  JOURNAL   Mol Biol Cell 24 (3), 194-209 (2013)
   PUBMED   23223568
  REMARK    GeneRIF: Splicing of SH3BP1 and CIN gene loci produces the novel
            brain specific splice variant BGIN.
REFERENCE   4  (bases 1 to 2012)
  AUTHORS   Irvin,M.R., Shrestha,S., Chen,Y.D., Wiener,H.W., Haritunians,T.,
            Vaughan,L.K., Tiwari,H.K., Taylor,K.D., Scherzer,R., Saag,M.S.,
            Grunfeld,C., Rotter,J.I. and Arnett,D.K.
  TITLE     Genes linked to energy metabolism and immunoregulatory mechanisms
            are associated with subcutaneous adipose tissue distribution in
            HIV-infected men
  JOURNAL   Pharmacogenet Genomics 21 (12), 798-807 (2011)
   PUBMED   21897333
REFERENCE   5  (bases 1 to 2012)
  AUTHORS   Huang,T.Y., Minamide,L.S., Bamburg,J.R. and Bokoch,G.M.
  TITLE     Chronophin mediates an ATP-sensing mechanism for cofilin
            dephosphorylation and neuronal cofilin-actin rod formation
  JOURNAL   Dev Cell 15 (5), 691-703 (2008)
   PUBMED   19000834
  REMARK    GeneRIF: Study demonstrates the ATP-sensitive interaction of the
            cofilin phosphatase chronophin (CIN) with the chaperone hsp90 to
            form a biosensor that mediates cofilin/actin rod formation.
REFERENCE   6  (bases 1 to 2012)
  AUTHORS   Kim,D.W., Eum,W.S., Choi,H.S., Kim,S.Y., An,J.J., Lee,S.H.,
            Sohn,E.J., Hwang,S.I., Kwon,O.S., Kang,T.C., Won,M.H., Cho,S.W.,
            Lee,K.S., Park,J. and Choi,S.Y.
  TITLE     Human brain pyridoxal-5'-phosphate phosphatase: production and
            characterization of monoclonal antibodies
  JOURNAL   J Biochem Mol Biol 38 (6), 703-708 (2005)
   PUBMED   16336786
  REMARK    GeneRIF: analysis of brain pyridoxal-5'-phosphate phosphatase
REFERENCE   7  (bases 1 to 2012)
  AUTHORS   Gohla,A., Birkenfeld,J. and Bokoch,G.M.
  TITLE     Chronophin, a novel HAD-type serine protein phosphatase, regulates
            cofilin-dependent actin dynamics
  JOURNAL   Nat Cell Biol 7 (1), 21-29 (2005)
   PUBMED   15580268
  REMARK    GeneRIF: We report the biochemical isolation of chronophin (CIN), a
            unique cofilin-activating phosphatase of the haloacid dehalogenase
            (HAD) superfamily and an important novel regulator of
            cofilin-mediated actin reorganization.
REFERENCE   8  (bases 1 to 2012)
  AUTHORS   Jang,Y.M., Kim,D.W., Kang,T.C., Won,M.H., Baek,N.I., Moon,B.J.,
            Choi,S.Y. and Kwon,O.S.
  TITLE     Human pyridoxal phosphatase. Molecular cloning, functional
            expression, and tissue distribution
  JOURNAL   J Biol Chem 278 (50), 50040-50046 (2003)
   PUBMED   14522954
  REMARK    GeneRIF: analysis of pyridoxal phosphatase cloning, expression and
            tissue distribution
REFERENCE   9  (bases 1 to 2012)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   10 (bases 1 to 2012)
  AUTHORS   Gao,G. and Fonda,M.L.
  TITLE     Identification of an essential cysteine residue in pyridoxal
            phosphatase from human erythrocytes
  JOURNAL   J Biol Chem 269 (11), 8234-8239 (1994)
   PUBMED   8132548
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            Z83844.5 and BC064922.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020315.4.
            
            Summary: Pyridoxal 5-prime-phosphate (PLP) is the active form of
            vitamin B6 that acts as a coenzyme in maintaining biochemical
            homeostasis. The preferred degradation route from PLP to
            4-pyridoxic acid involves the dephosphorylation of PLP by PDXP
            (Jang et al., 2003 [PubMed 14522954]).[supplied by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064922.1, SRR1163655.298190.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215904.7/ ENSP00000215904.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 Z83844.5           32673-32679
            8-2012              BC064922.1         1-2005
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..2012
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /note="pyridoxal phosphatase"
                     /db_xref="GeneID:57026"
                     /db_xref="HGNC:HGNC:30259"
                     /db_xref="MIM:609246"
     exon            1..634
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..951
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.74"
                     /note="pyridoxal phosphate phosphatase; PLP phosphatase;
                     pyridoxal (pyridoxine, vitamin B6) phosphatase; testicular
                     secretory protein Li 36"
                     /codon_start=1
                     /product="chronophin"
                     /protein_id="NP_064711.1"
                     /db_xref="CCDS:CCDS13953.1"
                     /db_xref="GeneID:57026"
                     /db_xref="HGNC:HGNC:30259"
                     /db_xref="MIM:609246"
                     /translation="MARCERLRGAALRDVLGRAQGVLFDCDGVLWNGERAVPGAPELL
                     ERLARAGKAALFVSNNSRRARPELALRFARLGFGGLRAEQLFSSALCAARLLRQRLPG
                     PPDAPGAVFVLGGEGLRAELRAAGLRLAGDPSAGDGAAPRVRAVLVGYDEHFSFAKLR
                     EACAHLRDPECLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYM
                     FECITENFSIDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSRLEEAQAYLAAGQH
                     DLVPHYYVESIADLTEGLED"
     exon            635..2012
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1990..1995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /note="hexamer: AATAAA"
     polyA_site      2012
                     /gene="PDXP"
                     /gene_synonym="CIN; dJ37E16.5; PLP"
                     /note="major polyA site"
ORIGIN      
        1 gagagcgcgc cgtgcccgcg gagagagcgc ggcgcgggag gccggcggcc ggccggctgc
       61 atggcgcgct gcgagaggct gcgcggagcg gccctgcgcg acgtgctggg ccgggcgcag
      121 ggggtcctgt tcgactgtga cggggtgctg tggaacggcg agcgcgccgt gccgggcgcc
      181 ccggagctgc tggagcggct ggcgcgggcc ggcaaggcgg ctctgtttgt gagcaacaac
      241 agccggcgcg cgcggcccga gctggccctg cgcttcgcgc gcctcggctt cggggggctg
      301 cgcgccgagc agctcttcag ctccgcgctg tgcgccgcgc gcctgctgcg ccagcgcctg
      361 cccgggcctc cggacgcgcc gggcgccgtg ttcgtgctgg gcggcgaggg gctgcgcgcc
      421 gagctgcgcg ccgcggggct gcgcctggcc ggggacccga gcgcggggga cggcgcggcc
      481 ccgcgcgtgc gcgccgtgct tgtgggctac gacgagcact tctccttcgc caagctgagg
      541 gaggcgtgcg cgcacctgcg cgaccccgag tgcctactcg tggccaccga ccgtgaccca
      601 tggcacccgc tgagcgacgg cagccggacc cctggcaccg ggagcctggc cgctgcagtg
      661 gagacagcct cgggacgcca ggccctggtg gtgggcaagc ccagccccta catgttcgag
      721 tgcatcacgg agaacttcag catcgacccc gcacgcacgc ttatggtggg tgaccgcctg
      781 gagaccgaca tcctctttgg ccaccgctgc ggcatgacca ctgtgctcac gctcacagga
      841 gtctcccgcc tagaagaggc ccaggcctac ctagcggccg gccagcacga cctcgtgccc
      901 cattactatg tggagagcat cgcagacttg acagaggggt tggaggactg agcccactgc
      961 acctgcagcc acaggcccac ccctccccac tccctgatcc cgtaggtgga ggcgatgggt
     1021 cacgagccat gttaagcaca accggctcct tggtccagtt ctgcaccggg gtggggctgg
     1081 gacccgggga aggtttgagg gcccttgcaa ccccctccca gcagtggctg ggcactcttt
     1141 gctgccccag aagctggtcc cctatggatt catcttggcc tgacccagcc aggtggcctt
     1201 atttcttccc tgtcacctcc cctccttgaa atctgggccc tggtgcctgc tgaagattcc
     1261 ctctatccct gagtacttag tcttctcccc cttccctggg gcttctagag ctctctctgc
     1321 ccctcaggtc ctggcccttg ggtccttgtc aaccagaggt ctagggaacc acaaccctta
     1381 ttgtcctggg tggaccaatc caaaggctaa gtgatagtga ctcatcaatg ttgggtcctg
     1441 tggggtacca gtttaggttc ctaagtaata gtgacccttt cacgtcctgg agcccgagtg
     1501 gaccaatcgg aagcctaagt gacgatgacc ctcgcatgtc ctaggtccct gggtggaccc
     1561 atcaggaccc caagtgatag tgacccagtg gtcttttaga tcctcagtag aacagtccag
     1621 aagcctgaga gacagtggcc ccttgataat ctgggtctca cgggaccagc taggggtcca
     1681 ggtttcagtc agtaaataag agtggtccac gtcctaaaga cacctctcct ttacaaagac
     1741 ttgtgatgct ctggggcttc tgtggccaag ccccaccctt tcctggtcat ggtacccgta
     1801 cagcgttgat ggccacagct cgaagggggg ctttcgtgtc cccctgtgcg gtcagtgttt
     1861 tcagtaccac ctctctcccg tgcccacttg gctatttact tatttattta ttgtgtgcca
     1921 gtgatggtgg gtgggggctg ggccttcccc gccacctcca cccctgttgt gacctgtcct
     1981 tccgtactta ataaagtgcg cgtgggagtg tt
//
