LOCUS       NM_003681               7341 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens pyridoxal kinase (PDXK), transcript variant 1, mRNA.
ACCESSION   NM_003681
VERSION     NM_003681.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Tan,W., Liu,B. and Ling,H.
  TITLE     [Pyridoxal kinase (PDXK) promotes the proliferation of serous
            ovarian cancer cells and is associated with poor prognosis]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 36 (6), 542-548 (2020)
   PUBMED   32696745
  REMARK    GeneRIF: [Pyridoxal kinase (PDXK) promotes the proliferation of
            serous ovarian cancer cells and is associated with poor prognosis].
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Mascolo,E., Barile,A., Mecarelli,L.S., Amoroso,N., Merigliano,C.,
            Massimi,A., Saggio,I., Hansen,T., Tramonti,A., Di Salvo,M.L.,
            Barbetti,F., Contestabile,R. and Verni,F.
  TITLE     The expression of four pyridoxal kinase (PDXK) human variants in
            Drosophila impacts on genome integrity
  JOURNAL   Sci Rep 9 (1), 14188 (2019)
   PUBMED   31578392
  REMARK    GeneRIF: The expression of four pyridoxal kinase (PDXK) human
            variants in Drosophila impacts on genome integrity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7341)
  AUTHORS   Chelban,V., Wilson,M.P., Warman Chardon,J., Vandrovcova,J.,
            Zanetti,M.N., Zamba-Papanicolaou,E., Efthymiou,S., Pope,S.,
            Conte,M.R., Abis,G., Liu,Y.T., Tribollet,E., Haridy,N.A.,
            Botia,J.A., Ryten,M., Nicolaou,P., Minaidou,A., Christodoulou,K.,
            Kernohan,K.D., Eaton,A., Osmond,M., Ito,Y., Bourque,P.,
            Jepson,J.E.C., Bello,O., Bremner,F., Cordivari,C., Reilly,M.M.,
            Foiani,M., Heslegrave,A., Zetterberg,H., Heales,S.J.R., Wood,N.W.,
            Rothman,J.E., Boycott,K.M., Mills,P.B., Clayton,P.T. and Houlden,H.
  CONSRTM   Care4Rare Canada Consortium and the SYNaPS Study Group
  TITLE     PDXK mutations cause polyneuropathy responsive to pyridoxal
            5'-phosphate supplementation
  JOURNAL   Ann Neurol 86 (2), 225-240 (2019)
   PUBMED   31187503
REFERENCE   4  (bases 1 to 7341)
  AUTHORS   Dolina,S., Margalit,D., Malitsky,S., Pressman,E. and Rabinkov,A.
  TITLE     Epilepsy as a pyridoxine-dependent condition: quantified urinary
            biomarkers for status evaluation and monitoring antiepileptic
            treatment
  JOURNAL   Med Hypotheses 79 (2), 157-164 (2012)
   PUBMED   22647618
  REMARK    GeneRIF: The affected pyridoxine metabolism is discussed as an
            inborn genetic trait in epilepsy in general, rather than a specific
            sign of pyridoxine-dependent epilepsy solely.
REFERENCE   5  (bases 1 to 7341)
  AUTHORS   Oshikawa,M., Tsutsui,C., Ikegami,T., Fuchida,Y., Matsubara,M.,
            Toyama,S., Usami,R., Ohtoko,K. and Kato,S.
  TITLE     Full-length transcriptome analysis of human retina-derived cell
            lines ARPE-19 and Y79 using the vector-capping method
  JOURNAL   Invest Ophthalmol Vis Sci 52 (9), 6662-6670 (2011)
   PUBMED   21697133
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7341)
  AUTHORS   Hanna,M.C., Turner,A.J. and Kirkness,E.F.
  TITLE     Human pyridoxal kinase. cDNA cloning, expression, and modulation by
            ligands of the benzodiazepine receptor
  JOURNAL   J Biol Chem 272 (16), 10756-10760 (1997)
   PUBMED   9099727
REFERENCE   7  (bases 1 to 7341)
  AUTHORS   Laine-Cessac,P. and Allain,P.
  TITLE     Kinetic studies of the effects of K+, Na+ and Li+ on the catalytic
            activity of human erythrocyte pyridoxal kinase
  JOURNAL   Enzyme Protein 49 (5-6), 291-304 (1996)
   PUBMED   9252787
REFERENCE   8  (bases 1 to 7341)
  AUTHORS   Zhang,Z., Smith,E., Surowiec,S.M., Merrill,A.H. Jr. and
            McCormick,D.B.
  TITLE     Synthesis of N-(4'-pyridoxyl)sphingosine and its uptake and
            metabolism by isolated cells
  JOURNAL   Membr Biochem 10 (1), 53-59 (1993)
   PUBMED   8510562
REFERENCE   9  (bases 1 to 7341)
  AUTHORS   Merrill,A.H. Jr., Henderson,J.M., Wang,E., McDonald,B.W. and
            Millikan,W.J.
  TITLE     Metabolism of vitamin B-6 by human liver
  JOURNAL   J Nutr 114 (9), 1664-1674 (1984)
   PUBMED   6088736
REFERENCE   10 (bases 1 to 7341)
  AUTHORS   Chern,C.J. and Beutler,E.
  TITLE     Biochemical and electrophoretic studies of erythrocyte pyridoxine
            kinase in white and black Americans
  JOURNAL   Am J Hum Genet 28 (1), 9-17 (1976)
   PUBMED   2009
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001052.1 and AP001053.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003681.4.
            
            Summary: The protein encoded by this gene phosphorylates vitamin
            B6, a step required for the conversion of vitamin B6 to
            pyridoxal-5-phosphate, an important cofactor in intermediary
            metabolism. The encoded protein is cytoplasmic and probably acts as
            a homodimer. Alternatively spliced transcript variants have been
            described, but their biological validity has not been determined.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU794642.1, SRR1803615.86988.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291565.9/ ENSP00000291565.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-253               AP001052.1         90927-91179
            254-308             AP001052.1         105867-105921
            309-413             AP001052.1         113465-113569
            414-497             AP001052.1         115522-115605
            498-544             AP001052.1         117877-117923
            545-630             AP001052.1         120793-120878
            631-676             AP001052.1         122298-122343
            677-788             AP001052.1         124316-124427
            789-925             AP001052.1         125381-125517
            926-992             AP001052.1         127496-127562
            993-3196            AP001052.1         127749-129952
            3197-7341           AP001053.1         1-4145
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..7341
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="pyridoxal kinase"
                     /db_xref="GeneID:8566"
                     /db_xref="HGNC:HGNC:8819"
                     /db_xref="MIM:179020"
     exon            1..253
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1105
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /EC_number="2.7.1.35"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pyridoxine kinase; pyridoxal (pyridoxine, vitamin B6)
                     kinase; pyridoxamine kinase; vitamin B6 kinase; epididymis
                     secretory sperm binding protein Li 1a"
                     /codon_start=1
                     /product="pyridoxal kinase isoform 1"
                     /protein_id="NP_003672.1"
                     /db_xref="CCDS:CCDS13699.1"
                     /db_xref="GeneID:8566"
                     /db_xref="HGNC:HGNC:8819"
                     /db_xref="MIM:179020"
                     /translation="MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFS
                     NHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELK
                     QQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGR
                     KIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMER
                     IRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKA
                     QAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL"
     exon            254..308
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            309..413
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            414..497
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            498..544
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            545..630
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            631..676
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            677..788
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            789..925
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            926..992
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     exon            993..7341
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1201
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="major polyA site"
     polyA_site      1256
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="major polyA site"
     regulatory      1283..1288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="hexamer: AACAAA"
     polyA_site      1301
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
     regulatory      3964..3969
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="hexamer: AATACA"
     polyA_site      3989
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="major polyA site"
     regulatory      7321..7326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
                     /note="hexamer: AATAAA"
     polyA_site      7341
                     /gene="PDXK"
                     /gene_synonym="C21orf124; C21orf97; HEL-S-1a; HMSN6C; PKH;
                     PNK; PRED79"
ORIGIN      
        1 gtcagaagga ggcgtctgcg ctgatcgggt ccgccgcgcg ccagagccag agtcgcagcc
       61 gaggggagcc ggggccggag cccgagcccg agccgagccg gagcccgagc gagcggcgga
      121 gaccgtgccc ccgcctcggc cccgcgccgc cgcggccagg cccggcatgg aggaggagtg
      181 ccgggtgctc tccatacaga gccacgtcat ccgcggctac gtgggcaacc gggcggccac
      241 gttcccgctg caggttttgg gatttgagat tgacgcggtg aactctgtcc agttttcaaa
      301 ccacacaggc tatgcccact ggaagggcca agtgctgaat tcagatgagc tccaggagtt
      361 gtacgaaggc ctgaggctga acaacatgaa taaatatgac tacgtgctca caggttatac
      421 gagggacaag tcgttcctgg ccatggtggt ggacattgtg caggagctga agcagcagaa
      481 ccccaggctg gtgtacgtgt gtgatccagt cttgggtgac aagtgggacg gcgaaggctc
      541 gatgtacgtc ccggaggacc tccttcccgt ctacaaagaa aaagtggtgc cgcttgcaga
      601 cattatcacg cccaaccagt ttgaggccga gttactgagt ggccggaaga tccacagcca
      661 ggaggaagcc ttgcgggtga tggacatgct gcactctatg ggccccgaca ccgtggtcat
      721 caccagctcc gacctgccct ccccgcaggg cagcaactac ctgattgtgc tggggagtca
      781 gaggaggagg aatcccgctg gctccgtggt gatggaacgc atccggatgg acattcgcaa
      841 agtggacgcc gtctttgtgg gcactgggga cctgtttgct gccatgctcc tggcgtggac
      901 acacaagcac cccaataacc tcaaggtggc ctgtgagaag accgtgtcta ccttgcacca
      961 cgttctgcag aggaccatcc agtgtgcaaa agcccaggcc ggggaaggag tgaggcccag
     1021 ccccatgcag ctggagctgc ggatggtgca gagcaaaagg gacatcgagg acccagagat
     1081 cgtcgtccag gccacggtgc tgtgagggcc ccgccgcttg cccgtgacac gcagcgcgtt
     1141 ggtgtctccg tgtttgtccc tgtgaaaaca tgtaacgtct gccttagagc catgaccgaa
     1201 acttgatatt tttttctttc atgagtgtcc ggcatctgct ggtcttcatt gtgaaacgtg
     1261 ccagtcgtgc tttgtgaaaa ataacaaagt ggtcacagaa atttgtgatc tgaaaacccg
     1321 gctcccttcc ccacaaggct cctgggcctc cgggaagacg ggcccctgtt tgccatctcg
     1381 ggggtgttcc ctgtgggagg gtgagtgggt gaggccgagc ctgctgcgtg tggagcctcg
     1441 agtgggccct ggctgccact accgtacaga ggccgtgtcg cgctgggctg ggcctgggtg
     1501 gcctctgtct ttgcatctct gagaaggagt cgggtggtaa cggttggggt caggaagaat
     1561 tctgccaagt atctttactg tcattctgac catagcctct ttgttcccgc attcgaactt
     1621 ttggttctta ctttgctgct cgtttagtcc ctggggattt cagatcttag gctgttgttt
     1681 caccgtatgg gagggttgat gtgagcttgc ttggagacac acggtgcagc atcagggacc
     1741 ttcccaggcc ccagcaaatt caagtcggtc tgcagacctc tcagctaccc gcgggacctc
     1801 ttgtaaccca tcggcatctt ccaggaatcc gccgagtgac ttgaggaaga tgctaacgca
     1861 gtaaggtctg tgctgggcca agagcagctt tgaagctcca gagaaccacc ccgtcaggtt
     1921 ccttgtggaa gctcccctca tccgtggtgc agcaggctga gcactgcgcg tttgccacgt
     1981 gctgcccgtg acagcacatt gagccacagc atttgtagac aggacagagg ggtgcctgcc
     2041 ccctgcccct gctggcacat ttaacccttg tcccctgacc tcagttctgt gccccaccaa
     2101 atgcccaggg gcaagaggcc accctggaag ctgccaatct tccaaggtgg gtgtggggca
     2161 cggtgggggc gggcagctcc caggcccttg ggcaggctgg ggtgacggca gaggccacag
     2221 caccagctct gacaagtcct atcatcctct gctcagcagt gacctccctg gccccacttt
     2281 gcccagagtt tggggtcccc ccaggtatag ctataggcgg cagtgcctgt ccctggcctg
     2341 ccttgatttc agccacaccc ctgcagccct gcatcccagc tctggggtgt gcagaggttt
     2401 gtgtctccag ggaacccacg gctggagaga aatagggaga tgcaggaagt gggggcccat
     2461 ggggccccca agaagcggac tctccaaggg gtacccccac cccgctacct tccccacgga
     2521 cgggcccctc ctggagccca taccctcctg tgaggccatt ccagtgtctt ctagaaagac
     2581 tcgcttgcca ggagtgcgtt ctttgttgaa aaatgccctg aagcgaaaag atgcaggttt
     2641 atatggaacc cccaccccct cccccactct cccactctgt tcgttctgaa tgtcttcacg
     2701 agcgtgcatc agggcgcctg gctcccccac ctcagccagt gagtcagaca cgggtttcgc
     2761 agccatgttt cctggctccg aggacacggg tggcaggccc gttgcagccc agagccactg
     2821 gtccctacag ggcgccgcca caccagcagg aaggaggatg gctgtgtccg gagcctggcg
     2881 gggaggcggc ctccccagta tgtgagtgca gggatctgcc agaaccacct ggccctctgt
     2941 agggcgttta actggaaata ccctcactgc caagtggaga ctggggcgtg tgccacattg
     3001 ccagccacca ggaaagcttt tctttttctt tttttttttt ttttaaacac caagagcacg
     3061 tatagcatgg gggaaagaac ctaaatgtct ctctgtcctg tgagctggtg aaaaacccag
     3121 catgagaacg cagtgtcagg tgtgggactc cttctgcccc tgcagtgggt gttacgggcg
     3181 gtgtgccctg gcgagcaagc tttgattctt ggttctttga gctcgtttca gaggctgagt
     3241 ccccacatca gctttagttc ttggacttcc ctgtattaag caagaattag gagaatggct
     3301 gtccctgcag gcgcctcccg taaatcctga gctctctggc gcaatctgaa acttctcttc
     3361 tgttttcttt ggctgtatca gccgaaccag gagaggcctg ggctgcgact aaggagaaag
     3421 aaatcggggg tttctgagag cagatggtgc ctttgtgggt gcagggcttt tgtggaaatt
     3481 gtcagcctct acgggcagag tccggcatcc cctccccaga ctgcctgctg tcaaaccacg
     3541 gagcagctgg agcctgccct gtccacggcc cgtttccacc cgggcatgtt cgtctctcct
     3601 gacttcggca gaggcccctg gtggccttca gtttcagttt ctcatccagg aaggtaacct
     3661 tgggcattgg cagtgggttt ccctatggct tggatccaga ttagaattga tctttgtttt
     3721 cactttccat agttaataac atgcaaaata atgagaagaa tttattttaa ggtgacagct
     3781 atactggtcc aacatcgcct gcttattgtc agggtacaga agtttaatac tttcttaatc
     3841 cagtttttca aacttctccc tgtagaccgt aaggatgaat tccacaatag gatccttttt
     3901 aaaatcgatt ttaaattgtt gcctagtcct gccaaggtta ttatgtgcat ctgttatttt
     3961 tccaatacat gtaaacagtt gcagcatgat gctttgttta atgtcctgtt cttaagctcg
     4021 ttagagccag ttttgaaacg tttggtctta ccgtgaacgg aggctggctt ggcttagcca
     4081 cgctgatgag taagtgaggg atgtctccat cttgagatca ccaggcaaga gagttgcctg
     4141 caccaggtaa gaggccaaag cccctggggt aacagtcccc accgctaccc gaggtaaaac
     4201 aataaaagct atgtggttga gctcaggcct ctcgtgcctg gtgtcagaga aggcagagcc
     4261 cacagtaggt gcacggtgca aggccctggg agggcactgg ccagggaagg tggtatagat
     4321 ggccctcaga ttgcggggcc ccgagcagct ccccactctg cccgtccacc ttccctggct
     4381 ccagcctcat tctctcttta gtttaactat gcaaagagag gaggttgaga gtgttctggc
     4441 agctggagct cttttccttg tccttcctgc cctccgatgg ggccacctgt gtcggggcag
     4501 cagtgtccat gtttatggag atcagaggtg tccccactgt gtggctggac tgtactctgc
     4561 tgcccgggta gccaggagtc tctccctctc tcccctgccg cctgcctggt ctcatgggcc
     4621 tccttcacac acccctccct gtggatcgcc tgcctgggcc cagagcaggg gaactggagt
     4681 ttgtgagtga gcagagcagg ttatgtgcag acagggaaac gagaactttg gacctggctt
     4741 tctgagtcca ggtgagagct gtgtggcccc ccgatgccac tctgcccgcc ggagggatgt
     4801 gcctgctgag ccttttcctt ccacgccgcc tctcactgcc aggccagcgg cttccgctga
     4861 gactcgctgg agaggcggct cccgtgtccg tccaccgagc actcagatgg atgctgatca
     4921 ccagggccga gggggctccc agaaggaccc caggccctgg ggagggtggc tgtgggaggc
     4981 caagtccact gcccggaagt cttgtcagcc ctaagccagg gaagcctgga gcgtggcctg
     5041 gcgggtctgg gtggacaccg tccccactcc ggactcccag cacaggggag gatacctgag
     5101 cctgtatggc cctgtagccc tgggcagagc tgggcctgtc gtgtgttcct gcctggcagg
     5161 tgcaggtgct ggccatctgc aggtggaagg aggtgggaat cttggatttt ttgttttttt
     5221 ttgttttttt ttttttgagg tgaagtctcg ctctgacacc caggctggcg tgcagtggtg
     5281 tgatctcggc tcactgcaaa ctccgcttcc tgggttcaag tggttctcct gccccagcct
     5341 cccaagtagc tgggattaca ggcatgcgcc accacgctca gctgattttt gtatttttag
     5401 tagagatggg gtttcaccat gttggccaag ctggtctcaa actcctgacc tcaagtgatc
     5461 tgcccgcctc ggcctcccag agtgctggga ttacaggcat gagccagtgc acccggcgga
     5521 atcttggaat ttttatagac agcacctcag tttctgactc cagccgcaca cctcctgcct
     5581 ctgccagcag gggttgccgc cagaccagag ccagggccag gtccctgcgt ccatcccccc
     5641 cggtaggatg gacgtgagcc atccttctag gggacttttt tcagtgtgcg actcgtctct
     5701 gttaggtggt aggagccagt ttgtgtggcc tgtgccacgc tccacagtgc gtggctgggc
     5761 tctgtgtgtg gcctgtgtcc cctgtccctg caggacccag caggcatcgt ggcgtgacag
     5821 ctgtgtccaa gccactgccc gggcatccca tcacccacca gggtgcacgg tctctcctgc
     5881 tgggggcttt ctgtcgcatg tgtgtctcct gtcgactctg cagtttgttc tcagagcaga
     5941 atgtttcctg ttctcagtgc acaaagacac tggttttcaa tcggcgtcta aaaccacgtt
     6001 cctgcctttc attgcaacac ggtgtgttca tttgtttaaa acagtttaat gagtaagttt
     6061 agatgactgg tcaatatctt aaaaatgtat attagtaaga agttcttcct ggaatttttc
     6121 tttcgattct ggcagaataa acaggtgttt ttagttttcc cactgtctga gccaagcagg
     6181 accctgtccc agagcaagag atgtcccctt ccatctctga cccttgcctg ggacaagctt
     6241 tgatgggggg ccccagcttc aaggctgtgg tgggaacagc acccccaaat gccagcctct
     6301 cctttcttcc catccaccag tatactgcgg ggccatttct ggtctttgtc caacaggaaa
     6361 cccatttctg gtgggatatg ccttccagtg ccacagggcc actcacccca tgcatctctg
     6421 tcctgcccgt cagtgctggg acggacagca agggcaagcc cagtgtctgg cggataggtg
     6481 ggtgggaaca gagaggggag aatgccgtcc taagcttctg cttggggatc ccccacacga
     6541 cctgggtact gcctgggaaa cctgtcctaa gtaaaactat ggacctcgcc tcgcccaccg
     6601 gcctgcgaag ccagcatctc cgtgaaggtg gatggaagcg cctttgtcct cattttgagc
     6661 tgcaagctgg gtcagcggct ctgaagccct cgagtgactt tctaacccaa gacccagccc
     6721 ctggcaggag gagggtgggt gcagggctgg tgggacaaaa agaggcctca gcaggcctgg
     6781 aagacccttc cagtacatcc cacagcgtgt cgagcagctg ggagaacctg tgtcaagctc
     6841 gagccgtcat aggtccccat gaggtgtctg aagccccttc ttggtgatgg gaggcagagg
     6901 tgctgacgtt ctggagcatg gacgtgagtc ctcagctggc tccgcgtggg cccttggagg
     6961 gtgccaggtg tgtggtgacc ttctggatgc ctttaacttc atggctgcgt cattcctgat
     7021 ttagaacttt aaccggagct tcatctagtg attgcaaaac tggaccaatg ggaggacggc
     7081 ggcgcagccc gctccctccg tggaatggag ctcagctctt cggaggcatc aaagcacctg
     7141 tcgcctccgt ggtccccctg ctgagggagt gcggcctctg caaggttcgg gggtggcttc
     7201 gtttgcctgg agtggccggc cctgcttgtg ccatgtggat gtttgtgagc ctcggtccta
     7261 cagcactgtg taggctgcat ctgtttcgtg ctggtcctgt tgacttgtat gatatccaca
     7321 aataaatatt ttcatggcgg t
//
