LOCUS       NM_015032               7472 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PDS5 cohesin associated factor B (PDS5B), mRNA.
ACCESSION   NM_015032
VERSION     NM_015032.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,L., Ma,T., Zhou,W., Li,J., Li,Y. and Xu,H.
  TITLE     [PDS5B inhibits the proliferation of A549 human lung cancer cells
            via downregulation of Wnt5a]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 39 (1), 15-20 (2023)
   PUBMED   36631010
  REMARK    GeneRIF: [PDS5B inhibits the proliferation of A549 human lung
            cancer cells via downregulation of Wnt5a].
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Zhang,N., Coutinho,L.E. and Pati,D.
  TITLE     PDS5A and PDS5B in Cohesin Function and Human Disease
  JOURNAL   Int J Mol Sci 22 (11), 5868 (2021)
   PUBMED   34070827
  REMARK    GeneRIF: PDS5A and PDS5B in Cohesin Function and Human Disease.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7472)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 7472)
  AUTHORS   Morales,C., Ruiz-Torres,M., Rodriguez-Acebes,S., Lafarga,V.,
            Rodriguez-Corsino,M., Megias,D., Cisneros,D.A., Peters,J.M.,
            Mendez,J. and Losada,A.
  TITLE     PDS5 proteins are required for proper cohesin dynamics and
            participate in replication fork protection
  JOURNAL   J Biol Chem 295 (1), 146-157 (2020)
   PUBMED   31757807
  REMARK    GeneRIF: PDS5 proteins are required for proper cohesin dynamics and
            participate in replication fork protection.
REFERENCE   5  (bases 1 to 7472)
  AUTHORS   Martino,J., Brunette,G.J., Barroso-Gonzalez,J., Moiseeva,T.N.,
            Smith,C.M., Bakkenist,C.J., O'Sullivan,R.J. and Bernstein,K.A.
  TITLE     The human Shu complex functions with PDS5B and SPIDR to promote
            homologous recombination
  JOURNAL   Nucleic Acids Res 47 (19), 10151-10165 (2019)
   PUBMED   31665741
  REMARK    GeneRIF: our study uncovers a protein complex, which consists of
            SWS1, SWSAP1, SPIDR and PDS5B, involved in DNA repair and provides
            insight into Shu complex function and composition.
REFERENCE   6  (bases 1 to 7472)
  AUTHORS   Losada,A., Yokochi,T. and Hirano,T.
  TITLE     Functional contribution of Pds5 to cohesin-mediated cohesion in
            human cells and Xenopus egg extracts
  JOURNAL   J Cell Sci 118 (Pt 10), 2133-2141 (2005)
   PUBMED   15855230
REFERENCE   7  (bases 1 to 7472)
  AUTHORS   Fallone,C.A.
  TITLE     Epidemiology of the antibiotic resistance of Helicobacter pylori in
            Canada
  JOURNAL   Can J Gastroenterol 14 (10), 879-882 (2000)
   PUBMED   11111111
  REMARK    Review article
REFERENCE   8  (bases 1 to 7472)
  AUTHORS   Geck,P., Maffini,M.V., Szelei,J., Sonnenschein,C. and Soto,A.M.
  TITLE     Androgen-induced proliferative quiescence in prostate cancer cells:
            the role of AS3 as its mediator
  JOURNAL   Proc Natl Acad Sci U S A 97 (18), 10185-10190 (2000)
   PUBMED   10963680
REFERENCE   9  (bases 1 to 7472)
  AUTHORS   Geck,P., Szelei,J., Jimenez,J., Sonnenschein,C. and Soto,A.M.
  TITLE     Early gene expression during androgen-induced inhibition of
            proliferation of prostate cancer cells: a new suppressor candidate
            on chromosome 13, in the BRCA2-Rb1 locus
  JOURNAL   J Steroid Biochem Mol Biol 68 (1-2), 41-50 (1999)
   PUBMED   10215036
REFERENCE   10 (bases 1 to 7472)
  AUTHORS   Couch,F.J., Rommens,J.M., Neuhausen,S.L., Belanger,C., Dumont,M.,
            Abel,K., Bell,R., Berry,S., Bogden,R., Cannon-Albright,L.,
            Farid,L., Frye,C., Hattier,T., Janecki,T., Jiang,P., Kehrer,R.,
            Leblanc,J.F., McArthur-Morrison,J., Meney,D., Miki,Y., Peng,Y.,
            Samson,C., Schroeder,M., Snyder,S.C., Simard,J. et al.
  TITLE     Generation of an integrated transcription map of the BRCA2 region
            on chromosome 13q12-q13
  JOURNAL   Genomics 36 (1), 86-99 (1996)
   PUBMED   8812419
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA856411.1, AB023196.2 and
            AL137201.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015032.3.
            
            Summary: This gene encodes a protein that interacts with the
            conserved protein complex termed cohesin. The cohesin complex holds
            together sister chromatids and facilitates accurate chromosome
            segregation during mitosis and meiosis. This protein is also a
            negative regulator of cell proliferation and may be a
            tumor-suppressor gene. [provided by RefSeq, Jul 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL137201.1, SRR1660805.165093.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315596.15/ ENSP00000313851.10
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                DA856411.1         26-78
            54-4565             AB023196.2         1-4512
            4566-7472           AL137201.1         4538-7444
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.1"
     gene            1..7472
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="PDS5 cohesin associated factor B"
                     /db_xref="GeneID:23047"
                     /db_xref="HGNC:HGNC:20418"
                     /db_xref="MIM:605333"
     exon            1..142
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            143..269
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..4505
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="androgen-induced shutoff 3; androgen-induced
                     proliferation inhibitor; androgen induced inhibitor of
                     proliferation; androgen-induced prostate proliferative
                     shutoff-associated protein AS3"
                     /codon_start=1
                     /product="sister chromatid cohesion protein PDS5 homolog
                     B"
                     /protein_id="NP_055847.1"
                     /db_xref="CCDS:CCDS41878.1"
                     /db_xref="GeneID:23047"
                     /db_xref="HGNC:HGNC:20418"
                     /db_xref="MIM:605333"
                     /translation="MAHSKTRTNDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMD
                     MDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDK
                     LKDIFMFITRQLKGLEDTKSPQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRT
                     LFSVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLA
                     KALLKRTAQAIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQ
                     LEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDIHVPIRLECV
                     KFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNF
                     VRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQIAWIKDKLLHIYYQNSID
                     DRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKALNEMWKCQNLLRHQVKD
                     LLDLIKQPKTDASVKAIFSKVMVITRNLPDPGKAQDFMKKFTQVLEDDEKIRKQLEVL
                     VSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEMIKFLLERIAPVHIDTESISALIKQ
                     VNKSIDGTADDEDEGVPTDQAIRAGLELLKVLSFTHPISFHSAETFESLLACLKMDDE
                     KVAEAALQIFKNTGSKIEEDFPHIRSALLPVLHHKSKKGPPRQAKYAIHCIHAIFSSK
                     ETQFAQIFEPLHKSLDPSNLEHLITPLVTIGHIALLAPDQFAAPLKSLVATFIVKDLL
                     MNDRLPGKKTTKLWVPDEEVSPETMVKIQAIKMMVRWLLGMKNNHSKSGTSTLRLLTT
                     ILHSDGDLTEQGKISKPDMSRLRLAAGSAIVKLAQEPCYHEIITLEQYQLCALAINDE
                     CYQVRQVFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQCLVKNINVRREY
                     LKQHAAVSEKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKDVKECLWFVLEILMA
                     KNENNSHAFIRKMVENIKQTKDAQGPDDAKMNEKLYTVCDVAMNIIMSKSTTYSLESP
                     KDPVLPARFFTQPDKNFSNTKNYLPPEMKSFFTPGKPKTTNVLGAVNKPLSSAGKQSQ
                     TKSSRMETVSNASSSSNPSSPGRIKGRLDSSEMDHSENEDYTMSSPLPGKKSDKRDDS
                     DLVRSELEKPRGRKKTPVTEQEEKLGMDDLTKLVQEQKPKGSQRSRKRGHTASESDEQ
                     QWPEEKRLKEDILENEDEQNSPPKKGKRGRPPKPLGGGTPKEEPTMKTSKKGSKKKSG
                     PPAPEEEEEEERQSGNTEQKSKSKQHRVSRRAQQRAESPESSAIESTQSTPQKGRGRP
                     SKTPSPSQPKKNVRVGRSKQAATKENDSSEEVDVFQGSSPVDDIPQEETEEEEVSTVN
                     VRRRSAKRERR"
     exon            270..473
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            474..560
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            561..658
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            659..785
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            786..866
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1007
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1123
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1218
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1364
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1516
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1630
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1712
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1761
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1901
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2017
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2123
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2284
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2408
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2567
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2568..2636
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2773
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2774..2897
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..3102
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3103..3217
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3350
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3470
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3471..3533
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3679
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3680..3785
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..4225
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            4226..4333
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4469
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     exon            4470..7472
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5336..5341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="hexamer: AATAAA"
     polyA_site      5360
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="major polyA site"
     regulatory      7240..7245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="hexamer: ATTAAA"
     polyA_site      7262
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
     regulatory      7455..7460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
                     /note="hexamer: AATAAA"
     polyA_site      7472
                     /gene="PDS5B"
                     /gene_synonym="APRIN; AS3; CG008"
ORIGIN      
        1 gccattttga agcggagagg aggaggaacg gcagggctgg ctgcggaagg ggaggggggg
       61 ggagaaggcg attggatgcg gcggcggcgg cggatcccgg agagccccgg agtgagcgga
      121 gtagcgagtc ggcaacccgg aggggtagaa atatttctgt catggctcat tcaaagacta
      181 ggaccaatga tggaaaaatt acatatccgc ctggggtcaa ggaaatatca gataaaatat
      241 ctaaagagga gatggtgaga cgattaaaga tggttgtgaa aacttttatg gatatggacc
      301 aggactctga agaagaaaag gagctttatt taaacctagc tttacatctt gcttcagatt
      361 tttttctcaa gcatcctgat aaagatgttc gcttactggt agcctgctgc cttgctgata
      421 ttttcaggat ttatgctcct gaagctcctt acacatcccc tgataaacta aaggatatat
      481 ttatgtttat aacaagacag ttgaaggggc tagaggatac aaagagccca caattcaata
      541 ggtattttta tttacttgag aacattgctt gggtcaagtc atataacata tgctttgagt
      601 tagaagatag caatgaaatt ttcacccagc tatacagaac cttattttca gttataaaca
      661 atggccacaa tcagaaagtc catatgcaca tggtagacct tatgagctct attatttgtg
      721 aaggtgatac agtgtctcag gagcttttgg atacggtttt agtaaatctg gtacctgctc
      781 ataagaattt aaacaagcaa gcatatgatt tggcaaaggc tttactgaag aggacagctc
      841 aagctattga gccatatatt accaattttt ttaatcaggt tctgatgctt gggaaaacat
      901 ctatcagcga tttgtcagag catgtctttg acttaatttt ggagctctac aatattgata
      961 gtcatttgct gctctctgtt ttaccccagc ttgaatttaa attaaagagc aatgataatg
     1021 aggagcgcct acaagttgtt aaactactgg caaaaatgtt tggggcaaag gattcagaat
     1081 tggcttctca aaacaagcca ctttggcagt gctacttggg caggtttaat gatatccatg
     1141 taccaatccg cctggaatgt gtgaaatttg ctagccattg tctcatgaac catcctgatt
     1201 tagcaaaaga cttaacagag tatcttaaag tgaggtcaca tgaccctgag gaagctatta
     1261 gacatgatgt tattgtgtca atagttacag ctgctaaaaa ggatattctt ctggtcaatg
     1321 atcacttact taattttgtg agagagagaa cattagacaa acgatggaga gtacgcaaag
     1381 aagccatgat gggacttgcc caaatttata agaaatatgc tttacagtca gcagctggaa
     1441 aagatgctgc aaaacagata gcatggatca aagacaaatt gctacatata tattatcaaa
     1501 atagtattga tgatcgacta cttgttgaac ggatctttgc tcaatacatg gttcctcaca
     1561 atttagaaac tacagaacgg atgaaatgct tatattactt gtatgccaca ctggatttaa
     1621 atgctgtgaa agcattgaat gaaatgtgga aatgtcaaaa tctgctccga catcaagtaa
     1681 aggatttgct tgacttgatt aagcaaccca aaacagatgc cagtgtcaag gccatatttt
     1741 caaaagtgat ggttattaca agaaatttac ctgatcctgg taaggctcag gatttcatga
     1801 agaaattcac acaggtgtta gaagatgatg agaaaataag aaagcagtta gaagtacttg
     1861 ttagtccaac atgctcctgc aagcaggctg aaggttgtgt gcgtgaaata actaagaagt
     1921 tgggcaaccc caaacagcct acaaatcctt tcctggaaat gatcaagttt ctcttggaga
     1981 ggatagcacc tgtgcacata gataccgaat ctatcagtgc tcttattaaa caagtgaaca
     2041 aatcaataga tggaacagca gatgatgaag atgagggtgt tccaactgat caagccatca
     2101 gagcaggtct tgaactgctt aaggtactct catttacaca tcccatctca tttcattctg
     2161 ctgaaacatt tgaatcatta ctggcttgtc tgaaaatgga tgatgaaaaa gtagcagaag
     2221 ctgcactaca aattttcaaa aacacaggaa gcaaaattga agaggatttt ccacacatca
     2281 gatcagcctt gcttcctgtt ttacatcaca aatctaaaaa aggacccccc cgtcaagcca
     2341 aatatgccat tcattgtatc catgcgatat tttctagtaa agagacccag tttgcacaga
     2401 tatttgagcc tctgcataag agcctagatc caagcaacct ggaacatctc ataacaccat
     2461 tggttactat tggtcatatt gctctccttg cacctgatca atttgctgct cctttgaaat
     2521 ctttggtagc tactttcatt gtgaaagatc ttctcatgaa tgatcggctt ccagggaaaa
     2581 agacaactaa actttgggtt ccagatgaag aagtatctcc tgagacaatg gtcaaaattc
     2641 aggctattaa aatgatggtt cgatggctac ttggaatgaa aaataatcac agtaaatcag
     2701 gaacttctac cttaagattg ctaacaacaa tattgcatag tgatggagac ttgacagaac
     2761 aggggaaaat tagtaaacca gatatgtcac gtctgagact tgctgctggg agtgctattg
     2821 tgaagctggc acaagaaccc tgttaccatg aaatcatcac attagaacaa tatcagctat
     2881 gtgcattagc tatcaacgat gaatgctatc aagtaagaca agtgtttgcc cagaaacttc
     2941 acaaaggcct ttcccgttta cggcttccac ttgagtatat ggcaatctgt gccctttgtg
     3001 caaaagatcc tgtaaaggag agaagagctc atgctaggca atgtttggtg aaaaatataa
     3061 atgtaaggcg ggagtatctg aagcagcatg cagctgttag tgaaaaatta ttgtctcttc
     3121 taccagagta tgttgttcca tatacaattc accttttggc acatgaccca gattatgtca
     3181 aagtacagga tattgaacaa cttaaagatg ttaaagaatg tctttggttt gttctggaaa
     3241 tattaatggc taaaaatgaa aataacagtc acgcttttat cagaaagatg gtagaaaata
     3301 ttaaacaaac aaaagatgcc caaggaccag atgatgcaaa aatgaatgaa aaactgtaca
     3361 ctgtgtgtga tgttgccatg aatatcatca tgtcaaagag tactacatac agtttggaat
     3421 ctcctaaaga cccggtacta ccagctcgtt tcttcactca acctgacaag aatttcagta
     3481 acaccaaaaa ttatctgcct cctgaaatga aatcattttt cactcctgga aaacctaaaa
     3541 caaccaatgt tctaggagct gttaacaagc cactttcatc agcaggcaag caatctcaga
     3601 ccaaatcatc acgaatggaa actgtaagca atgcaagcag cagctcaaat ccaagctctc
     3661 ctggaagaat aaaggggagg cttgatagtt ctgaaatgga tcacagtgaa aatgaagatt
     3721 acacaatgtc ttcacctttg ccggggaaaa aaagtgacaa gagagacgac tctgatcttg
     3781 taaggtctga attggagaag cctagaggca ggaaaaaaac gcccgtcaca gaacaggagg
     3841 agaaattagg tatggatgac ttgactaagt tggtacagga acagaaacct aaaggcagtc
     3901 agcgaagtcg gaaaagaggc catacggctt cagaatctga tgaacagcag tggcctgagg
     3961 aaaagaggct caaagaagat atattagaaa atgaagatga acagaatagt ccgccaaaaa
     4021 agggtaaaag aggccgacca ccaaaacctc ttggtggagg tacaccaaaa gaagagccaa
     4081 caatgaaaac ttctaaaaaa ggaagcaaaa aaaaatctgg acctccagca ccagaggagg
     4141 aggaagaaga agaaagacaa agtggaaata cggaacagaa gtccaaaagc aaacagcacc
     4201 gagtgtcaag gagagcacag cagagagcag aatctcctga atctagtgca attgaatcca
     4261 cacagtccac accacagaaa ggacgaggaa gaccatcaaa aacgccatca ccatcacaac
     4321 caaaaaaaaa tgtccgtgta ggacgctcca aacaagcagc tactaaggaa aatgattcaa
     4381 gtgaagaagt agatgtgttt cagggtagct ctcctgtcga tgatattcca caggaagaaa
     4441 cagaggagga ggaagtttct acagtaaatg tacggcggcg aagtgctaaa agggaacggc
     4501 gatgaacaaa tgtaattaat aactttctct gtgaaagctt tggaaaaatc tttttttttt
     4561 tttttggtca agcttgaggc tgaataaagc ctttgatgca caaaatggga ctgctgaaga
     4621 gtggacagtt ggaccttact ttggtgaccc catacatttg tggtcacatg ctttagccat
     4681 acacatggta acattgacta tggagtcttg tgaaagtgta atgtgcgatg gctatgtaga
     4741 cataaagaag aaacttgtaa atatcttttt tctttttttt aatgtttctg atttctgaag
     4801 tgcttgtata gcttttatct gcggctttaa actgacagta cccgactgtt tattggatct
     4861 attgatttga aaagaatttg ttaggataga tcttaagcag taatctgtca gtgtttgtat
     4921 ttgtattctc tgcaatttta ctgtgaaaaa aaatttgttt tcaacaattg gtgtcatttt
     4981 cttgatgtca ctatttgttg gagagttaaa tggtctcttc cctttgtgta tcttacctag
     5041 tgtttactcc tgggcaccct taatcttcag aggtgctaaa ttgtctgcca ttacaccaga
     5101 aggatgcctc tgataggagg acaaccatgc aaattgtgaa atagtcctga agttcttgga
     5161 ttactttaca cctcagtatt gatttgtccc agaattttct ggcctttcat ggcaatgaaa
     5221 attttaagaa gaaagattta aagtatttta attttaaaga gtgtgttata aaataatgta
     5281 ctgaattctt tatcccattt tatcatcctt tcagttttta ttaatctact gtatcaataa
     5341 aattctgtaa tttgaatgag tttttaatag tctagaatgt tattgtgtat agatatttcc
     5401 tcttgaacgt tatgttcaga aaatgcaaat tacactataa tataaaacct gatatataca
     5461 cattagaaat attccagttc tccgtaacag tgtaaagtta atcagaaaga aaaaatttta
     5521 ttgatgcagt gtgtctttat aaagctgttc ttgaaagcca agtgttttgt attttatagt
     5581 gagttgcatg tttttgaaaa aaagtgctga aaatgggatg tgctgttttc tgtaaattca
     5641 tatttagcca tagtatatga tagattgccc cataacatag tttttcatca agagtttatt
     5701 attgtacttt attttggagc caaaaaattg attctggggg gtgggggcag cgtagaagtg
     5761 gtatatccaa gtatatcagc tactgtagtt gtcacagtct tgtgaacttt gaatgaaatt
     5821 ccactttgtc cagattggga gaagtggaaa tttatttgga tttagagcag gtcttttttt
     5881 ttcttcattg taatctgcaa aatgtagaaa taaattactt aaggcagtat cctttataca
     5941 gttgtataaa ctgtattttg aaccaaaaca tataggttac tacttaatgc ttacctaatt
     6001 tcattttagt attttaggtg ctgttatgtt ttttcatggt taacaccaga ggggaaaaaa
     6061 tcatattcta acttattaaa tttatcacat tgaatagtgg aacattttca tatgatacac
     6121 cattatgttt aagatatatt tccaagattg gttataatag atggggcata taataaagaa
     6181 gctactattt tggaaaatga cattttacta tttgccaatg tatattgcaa ttgatgtgat
     6241 tatttttctt ttaaagattt gtttgtgttt atgaaacagc catttatttt ttaaaaagtg
     6301 ttttgaattg tgtcttaatc tctccatatt caactattct tcatttacaa caatagtgac
     6361 accagtaatt atgagaggct gtcatatatc aaagcagctc aggtgggatc aacatacatt
     6421 ggttttgaaa tgtttcctta gctcggttct cccaaacaca taatttctgt ttcagcagta
     6481 caaaggcatg ttttaaaatc tataacataa agaataagga atagctttgt atttttgtca
     6541 ttgattaaat tgcaccagaa atgtttatgg aaatattaag aacattttat aaaacatacg
     6601 aaaaagtgat tgtgaaggat ttttatttgc atgattatag ttaagcaaat ttcatttcac
     6661 atttttgtaa tgctgtacag cagttcacaa aaaaatgttc attgtagatt ttgttatgtt
     6721 caatgccaat gagtctgtaa ttttacgacc tgtctgttct ttttttgggt ggattacgat
     6781 gtaaattttt cttttctttc tttctttttt tttttttttg tgtagaaaaa taggtgcaat
     6841 aatgatcaaa gttttgatgt cttgagtctc catcttaggg gattatctta cgtttaagct
     6901 taacatttca tgtagtaaga tttggagagc cacatacttt acagtaaaat taagtgtgta
     6961 taaaacatta attgctaaca attgttagca aactatttca gtgataatgt tcattttgaa
     7021 aatatgtact gtataacatt aagaaaatat ttaactccct gtaacaagtt ccattatgaa
     7081 aatcttattc ctcagtgagg ttatcttgct gcactctgta gcaaatttgt ttaatctaca
     7141 ttataataaa atttcttgct gcagtgcaac aggaggcttt ttcagtgatc ttcactgtat
     7201 atgtaaatta caaatgtggc tgtaaaactt attccaggta ttaaaggtta aattgctttc
     7261 tatatcttct tataacttgt aagtctgatt tttaagattt ccttttgtcc acttgtaaga
     7321 atgtcaggaa tttaagaatt tgtttaaatt aggcatatct ctgccatcac atagtattat
     7381 gtcaccataa tgaacaattg ctatttaaat agataaccaa ttttcagaca catttttgga
     7441 tttctgtgaa gttgaataaa cataaaagct aa
//
