LOCUS       NM_017990               8549 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens pyruvate dehydrogenase phosphatase regulatory subunit
            (PDPR), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_017990 XM_942678 XM_946219
VERSION     NM_017990.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Brock,P., Sevigny,M., Liyanarachchi,S., Comiskey,D.F. Jr., Li,W.,
            Saarinen,S., Yilmaz,A.S., Nieminen,A.I., Ringel,M.D., Peltomaki,P.,
            Ollila,S. and Nieminen,T.T.
  TITLE     PDPR Gene Variants Predisposing to Papillary Thyroid Cancer
  JOURNAL   Thyroid 34 (5), 575-582 (2024)
   PUBMED   38062777
  REMARK    GeneRIF: PDPR Gene Variants Predisposing to Papillary Thyroid
            Cancer.
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 8549)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8549)
  AUTHORS   Chen,X., Li,X., Wang,P., Liu,Y., Zhang,Z., Zhao,G., Xu,H., Zhu,J.,
            Qin,X., Chen,S., Hu,L. and Kong,X.
  TITLE     Novel association strategy with copy number variation for
            identifying new risk Loci of human diseases
  JOURNAL   PLoS One 5 (8), e12185 (2010)
   PUBMED   20808825
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8549)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   6  (bases 1 to 8549)
  AUTHORS   Sugden,M.C. and Holness,M.J.
  TITLE     Recent advances in mechanisms regulating glucose oxidation at the
            level of the pyruvate dehydrogenase complex by PDKs
  JOURNAL   Am J Physiol Endocrinol Metab 284 (5), E855-E862 (2003)
   PUBMED   12676647
  REMARK    Review article
REFERENCE   7  (bases 1 to 8549)
  AUTHORS   Ohara,O., Nagase,T., Mitsui,G., Kohga,H., Kikuno,R., Hiraoka,S.,
            Takahashi,Y., Kitajima,S., Saga,Y. and Koseki,H.
  TITLE     Characterization of size-fractionated cDNA libraries generated by
            the in vitro recombination-assisted method
  JOURNAL   DNA Res 9 (2), 47-57 (2002)
   PUBMED   12056414
REFERENCE   8  (bases 1 to 8549)
  AUTHORS   Lawson,J.E., Park,S.H., Mattison,A.R., Yan,J. and Reed,L.J.
  TITLE     Cloning, expression, and properties of the regulatory subunit of
            bovine pyruvate dehydrogenase phosphatase
  JOURNAL   J Biol Chem 272 (50), 31625-31629 (1997)
   PUBMED   9395502
REFERENCE   9  (bases 1 to 8549)
  AUTHORS   Teague,W.M., Pettit,F.H., Wu,T.L., Silberman,S.R. and Reed,L.J.
  TITLE     Purification and properties of pyruvate dehydrogenase phosphatase
            from bovine heart and kidney
  JOURNAL   Biochemistry 21 (22), 5585-5592 (1982)
   PUBMED   6293549
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009022.10 and AC009060.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 14, 2019 this sequence version replaced NM_017990.4.
            
            Summary: Pyruvate dehydrogenase complex (PDC) catalyzes the
            oxidative decarboxylation of pyruvate and links glycolysis to the
            tricarboxylic acid cycle and fatty acid synthesis. The
            dephosphorylation and reactivation of PDC is catalyzed by pyruvate
            dehydrogenase phosphatase (PDP). The dimeric PDP has a catalytic
            subunit and a regulatory subunit. This gene encodes the
            FAD-containing regulatory subunit of PDP. The encoded protein acts
            to decrease the sensitivity of the PDP catalytic subunit to
            magnesium ions. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jan
            2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB082521.1, SRR11853563.12882.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288050.9/ ENSP00000288050.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AC009022.10        28797-28905         c
            110-219             AC009022.10        28307-28416         c
            220-478             AC009022.10        22518-22776         c
            479-612             AC009022.10        15844-15977         c
            613-694             AC009022.10        14372-14453         c
            695-858             AC009022.10        14115-14278         c
            859-980             AC009022.10        12693-12814         c
            981-1098            AC009022.10        11818-11935         c
            1099-1248           AC009022.10        10937-11086         c
            1249-1441           AC009022.10        6851-7043           c
            1442-1566           AC009022.10        4214-4338           c
            1567-1722           AC009022.10        848-1003            c
            1723-1856           AC009022.10        551-684             c
            1857-2005           AC009060.7         44787-44935
            2006-2118           AC009060.7         45698-45810
            2119-2213           AC009060.7         47411-47505
            2214-2303           AC009060.7         49741-49830
            2304-2486           AC009060.7         54668-54850
            2487-8549           AC009060.7         57752-63814
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..8549
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /note="pyruvate dehydrogenase phosphatase regulatory
                     subunit"
                     /db_xref="GeneID:55066"
                     /db_xref="HGNC:HGNC:30264"
                     /db_xref="MIM:617835"
     exon            1..109
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            110..219
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            220..478
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..2891
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /EC_number="1.5.8.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial"
                     /codon_start=1
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial isoform 1 precursor"
                     /protein_id="NP_060460.4"
                     /db_xref="CCDS:CCDS45520.1"
                     /db_xref="GeneID:55066"
                     /db_xref="HGNC:HGNC:30264"
                     /db_xref="MIM:617835"
                     /translation="MMFYRLLSIVGRQRASPGWQNWSSARNSTSAAEARSMALPTQAQ
                     VVICGGGITGTSVAYHLSKMGWKDIVLLEQGRLAAGSTRFCAGILSTARHLTIEQKMA
                     DYSNKLYYQLEQETGIQTGYTRTGSIFLAQTQDRLISLKRINAGLNVIGIPSEIISPK
                     KVAELHHLLNVHDLVGAMHVPEDAVVSSADVALALASAASQNGVQIYDRTSVLHVMVK
                     KGQVTGVETDKGQIECQYFVNCAGQWAYELGLSNEEPVSIPLHACEHFYLLTRPLETP
                     LQSSTPTIVDADGRIYIRNWQGGILSGGFEKNPKPIFTEGKNQLEIQNLQEDWDHFEP
                     LLSSLLRRMPELETLEIMKLVNCPETFTPDMRCIMGESPAVQGYFVLAGMNSAGLSFG
                     GGAGKYLAEWMVHGYPSENVWELDLKRFGALQSSRTFLRHRVMEVMPLMYDLKVPRWD
                     FQTGRQLRTSPLYDRLDAQGARWMEKHGFERPKYFVPPDKDLLALEQSKTFYKPDWFD
                     IVESEVKCCKEAVCVIDMSSFTKFEITSTGDQALEVLQYLFSNDLDVPVGHIVHTGML
                     NEGGGYENDCSIARLNKRSFFMISPTDQQVHCWAWLKKHMPKDSNLLLEDVTWKYTAL
                     NLIGPRAVDVLSELSYAPMTPDHFPSLFCKEMSVGYANGIRVMSMTHTGEPGFMLYIP
                     IEYALHVYNEVMSVGQKYGIRNAGYYALRSLRIEKFFAFWGQDINNLTTPLECGRESR
                     VKLEKGMDFIGRDALLQQKQNGVYKRLTMFILDDHDSDLDLWPWWGEPIYRNGQYVGK
                     TTSSAYSYSLERHVCLGFVHNFSEDTGEEQVVTADFINRGEYEIDIAGYRFQAKAKLY
                     PVASLFTQKRRKDDMELSDLHGK"
     exon            479..612
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            613..694
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            695..858
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            859..980
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1098
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1248
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1441
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1566
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1722
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1856
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2005
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2118
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2213
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2303
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2304..2486
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..8549
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8523..8528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /note="hexamer: AATAAA"
     polyA_site      8549
                     /gene="PDPR"
                     /gene_synonym="PDP3"
                     /note="major polyA site"
ORIGIN      
        1 atccccgaac cccgctttcc ggcccgcggc gaccccaggc aactgttgtg gctgccgcat
       61 tgctcccgcc gggctgtagc tgagcgcgga gcccgtgggg accggtgaga tggaactgtt
      121 ttcccgcgtt gagacgtgcg gtccgcttgt gcttttagaa ctaaagactg ctgcagagtc
      181 cggaggaaga agtcacctag aaaaatctgg gacagggcat ttgagttcct gagatctagt
      241 tggtgagaga catgatgttc taccggttgc tgtcgattgt tggaagacaa agagccagcc
      301 caggatggca gaactggtcc tctgcaagaa acagcacgtc agctgccgag gcgcgttcca
      361 tggccctgcc cacccaggca caggtggtca tctgtggagg tggaatcacg ggcacttctg
      421 tggcctatca cctctccaaa atggggtgga aggatattgt ccttttggag cagggcaggc
      481 tggctgctgg ctctaccagg ttctgtgctg gcatcctgag cactgccagg cacttgacca
      541 ttgagcagaa gatggcagac tactcaaaca aactctacta tcagttagag caagaaacag
      601 ggatccaaac aggttacaca aggacaggct caatctttct ggcccaaact caggaccgac
      661 tgatctccct gaagcgcatc aacgcagggc tgaatgttat aggtatccct tctgagatca
      721 tctcccccaa gaaagtggcc gagcttcacc atctcctcaa cgtgcacgac ctggtggggg
      781 ccatgcatgt tcccgaggat gcagtggtgt cttccgctga cgtggctctt gccctggcaa
      841 gtgctgcctc ccaaaatggt gttcagatct atgaccggac atctgttctt catgtaatgg
      901 tcaaaaaagg tcaagttact ggagtggaga ccgataaagg acagattgaa tgccagtatt
      961 ttgtcaactg tgctggccag tgggcatacg agctgggtct gtccaacgag gagccggtta
     1021 gtatcccgct acatgcctgc gaacacttct acctcctgac tcgccccttg gagacccctc
     1081 tgcagagcag cacaccaact attgtggatg ctgatggaag aatttatatt cggaactggc
     1141 agggtggcat cctgtctggg ggctttgaga agaacccgaa accaattttc actgagggca
     1201 agaaccagct ggagattcag aatctacagg aagactggga tcactttgag cctctgttga
     1261 gttcccttct gaggaggatg ccagaattag agactctgga gatcatgaag ttggtgaact
     1321 gcccagagac cttcacacca gacatgaggt gcatcatggg cgagtctcct gcagtgcagg
     1381 gctactttgt cctggcagga atgaactctg ctggcctttc atttggtgga ggagccggaa
     1441 agtaccttgc cgaatggatg gtacatggtt atccctcaga aaacgtttgg gaattggacc
     1501 tgaaacgttt tggagccctc cagagcagcc gcacctttct gcgccaccgg gtcatggaag
     1561 tcatgccttt gatgtatgat ctgaaggttc cccgctggga cttccagacc ggtaggcagt
     1621 tacgcacctc tcctctctac gaccggctgg atgcacaggg agccaggtgg atggagaaac
     1681 atggatttga gaggccaaag tactttgttc cccccgacaa ggacctcctg gcattggagc
     1741 agagcaagac tttctataag ccagattggt ttgacatcgt ggagtctgaa gtcaagtgct
     1801 gtaaggaagc tgtgtgtgtc attgacatgt cctctttcac aaagtttgag ataacatcca
     1861 ctggggatca ggcattagaa gttctacagt acctcttctc caatgacctg gatgtgcctg
     1921 tgggccacat tgtgcatact ggcatgctca acgagggtgg agggtatgaa aatgactgca
     1981 gcatagcacg actgaacaag cgcagtttct tcatgatctc tccaaccgac cagcaggtcc
     2041 actgttgggc ctggcttaag aaacacatgc cgaaagacag caacctgctc ctggaggacg
     2101 tcacctggaa gtacacagcc ctcaatctga ttggccctcg agctgtggat gtgctgtctg
     2161 agttgtccta tgcccctatg actccagacc acttcccaag cctcttttgc aaggagatga
     2221 gtgtgggcta tgcaaatggg atccgggtga tgagcatgac gcacacagga gagccaggat
     2281 tcatgctcta catccccata gagtacgccc tgcatgtata caatgaagtg atgagtgttg
     2341 gccagaaata cggaatccgg aatgctgggt attacgctct tcgcagtctc cgaattgaga
     2401 agttttttgc cttctggggt caggatataa ataacctcac cacgcccctg gaatgtggac
     2461 gagagtctcg ggtgaaatta gagaagggca tggatttcat tggtcgcgac gccctcctgc
     2521 agcagaagca gaatggagtg tataaacgcc tcaccatgtt catcctggac gaccatgatt
     2581 cagacctaga cctttggcct tggtggggag agcccattta ccggaatggg cagtatgttg
     2641 gcaagaccac cagcagtgcc tacagctaca gcctggagcg ccacgtttgc ctgggctttg
     2701 tgcacaattt ttctgaggac acgggggaag agcaagtggt gacagcagat ttcatcaacc
     2761 ggggagagta tgagattgac atcgcgggat accgcttcca ggccaaggcc aagctctacc
     2821 ctgtcgcctc cctcttcacc cagaagcgcc gaaaggatga catggagctg agtgacttac
     2881 atgggaagtg atgccaccag ggcagcctca cctcctcccc atcatcttgt cctagagtgg
     2941 gcgtcacctt ggagcttctc ttccttccgc ctctgttcct cttctggagc ctttgcctcc
     3001 catctcttat ctccttgata taattttgaa cttgacctac tttaaacttt tttgctctgc
     3061 agccttcctt gcccttccac ctcctcctcc taatattcac tctgggctct tcttcccttc
     3121 ccacccctca ctcagcttct cgtggtggca ggaggtatgt ctgacaggac agaagcaagc
     3181 tccactgtgg acatgagtga tggtgacagc tgctttcaaa ttctgcatct caaggcaggg
     3241 caagccgggg tggtgcaggt ctcagggcac gagtccctct cccttgggtt ccattttctt
     3301 ggagcagcct attagttctg gtggggttgc cgtgtgtcgg atgcagccct gaggggcagc
     3361 tcgtgcctgc agcccggcag ctcgttctcc tgttctgctg tgctgtgggc tggcactcga
     3421 tacctctggc aagaggatga ttatctacct cactgataag aggcatcgca tggacgttct
     3481 ctggtctgta gtggagacaa gcagttaacc tagcaccatc ctcatcctcc ctgcagaagc
     3541 cattagcaga cctttgggcc agggcagcct ccctataatt ttcttcatct cttgctaatc
     3601 tctataataa ggttgctttt tctttcttag ttgagtaaac aagtaagcca cagtgtttga
     3661 aagggaaaaa ccatattgcc ttgtgtgttg cttttcccag tcaaaagggt ctgaccttag
     3721 aaagtcccca ccagtgatgc tgagtgttct gctatggaaa caatgctgct tttctctcag
     3781 ctgtccaaag ttcagccgtt cctccttccc cttctcccag tgcgtttgac tttgtcaggt
     3841 cagcctcact tgtccctttc acagttggcc cataaagtca tgtttctctc cctttagaat
     3901 agggagggga agggatcagg tgtgttgtcc tgggcctttc aaccttgcgg gctgtgctga
     3961 gttccagaga gctgccggaa ttccaggttt gactctccag cacagagcag agctacacag
     4021 aggtgaccca cgttagtcca ctgagagttc tgagtccaaa gggtgtacgt tgcataaggc
     4081 tagagaccgc ttttcctcct ttccccccag gctctttgtt tccctccacc cacccttcca
     4141 tatcctctgg agcaggagac aatgtgggtg cccagaagtg ccctgcgctt tcaggcagtg
     4201 tctctgtttt cccaaggtga aacttagata tcatggactg ttgtccagcc tcaatgcttc
     4261 attttcccct tgcatatcaa ctgccctaat ctgacttttt ttaagtgcaa ataaactgct
     4321 ataagacatt ttggttagta aaactctagc ctttggcgtg ttggtatctt ggaagcatta
     4381 gctctgatag gtctataagt gtattaaggg acatccttcc atttcattag agcagcttaa
     4441 aatgctcagg tgtctgcctt ctctggtgtt tctttgggat ctctgtttac ccacttacgc
     4501 tggctccttc tagacatttc ttgccacttc cccaactaga ccactcctag tgattactga
     4561 ctttagtgcc taaacctttt tggaaggttc tggttggctt gttagaggag catatgatta
     4621 gaattctgtt tgggggtcta gttgtcttgg agcaagtatg tacttaacta gccgagagat
     4681 gctgcctcta ggcagagagg aggagaggtg ttgccggctg gagggccaac cagtgaacac
     4741 cagctgtgaa ggccttggga gaggaggaac aggcccttgg gcagatgcag gcattaccag
     4801 cagggagcag acttacctcc gaagatggag acaggtgact gagagctgca ggcctcctct
     4861 gctcttccaa acacgtagca tttgcacccc tccaaagcca tctttgtaaa ggaaaacgta
     4921 tttgtaattg aatccagaag aatttagtta cacatagaca taactcttca accttaacta
     4981 tggcaataca tttgtgcttt aactgttaca tagcagtatc accacttacc aggatccaaa
     5041 tcgaaataat aaaagctgtc tccatagttt aaaatcgaat agtgccatca tcacagtata
     5101 ttagtcaaat agaagcttca tcagaaatgt atcccacata gagttttaag acttggattc
     5161 tcttctgccc ttgttaatct ccaactaatt actacagatt gacacgtttt taattagctg
     5221 tcctttgtaa gaagtcagga aatctgatgc tgtgtccaaa attatgcact gtttgttgaa
     5281 gtagaaccag aaatcctgac ctcctgttaa atgacatcag tttccccctc tgagcaacag
     5341 actgcttgtc ttgctaggag aggaggatgg ggggctgagc actcaggctg tccattgaaa
     5401 ccccttgtcc atgaataggg tcatactcct aagactgatg gggtgttgat cttctaggac
     5461 atcacttgtt tattcagtgc cccaaacaca gatttctctt ctagcacttt agagttgatc
     5521 cttgaagtct ctcctggttc attcaaatac aagctgtgtg agtctggtgg ttttctgtga
     5581 ttggtctaat gtgagctctt tgaacagaca gatctgacag tgaatgactc tcccctgctt
     5641 ctggcataac tgctttgcct ctgtctagtg tccaagcatc ttagctgttc aagaggagag
     5701 ggcagcataa cttcctgacc accggtgtca gatatcagag cattctggac tcctgagagg
     5761 cagtggcctc ttgagtgaac aggggaggcc agtagatgcc ccagatccag agccgtggct
     5821 gcaaatccag caggaataag gagggacaac cacagcctcc tcatccatgt gtcatttcca
     5881 agggtttgcc ttgtgtctca gctcattctg ggcagcacgt ttgtcttctg tccctagaga
     5941 tttgaaggat tttggactct tgtgaatggg tgactggact tggctttaca gagttgggtg
     6001 cttttttctc tctgcaatta cctgtcatag cattttgtgc tcaccacgaa ggatggtctc
     6061 tgccttctct tgtcggtgta tgccatctga acctaggaac acaaagtata ttggcctcaa
     6121 acggagaccc agggttgcca gttttccgtg ggccttcccc tcccttgaaa tgtctttaat
     6181 tacctcccct tcatcgtcag gccacgtgtg acttctgttc ttagcactgc cagggtcatt
     6241 gacttccatc taagcttgca tcaggaagat gttccttctg tgatcattgg tactgaagcc
     6301 agaaaagctc tcattcagga actctgaaga gcaaaaaggg acaaacacta actgctgagc
     6361 tgggccattt gatctccttt caccttgcat tgctgtcaca gcaccttgta tgatggcagg
     6421 acaggctcca gcagagagaa ctgcacagtg accactgtat ttttcacgct cttccaggga
     6481 tccctgtccc ccgacattga agagatctca ttcaggccag agacacagag accacatagc
     6541 ccagtgatta aaccccggtt tcactctggc cccaggagtg gagcctggcc actcctgttt
     6601 ggttctcact gggaggccca ctggccttgg atcatctcct catgcacacc cggagtttta
     6661 cctgcttgct tgctttcctg gactgctgtt tgcaagaaag taactaaaac atgaaaagta
     6721 aacctccagc ttccacagta tattacctgc cgttgcatgc atttgaaagt tagcctcctc
     6781 ccttgccacc gtcttggtgg cagtagcgat gcaagaatga tgggagcttt ccgagagcgt
     6841 tcagtgtttc actgaagaca ggacccatag ccttcatttc tggctctgtg tctcctctgg
     6901 catatggaca catttcctgg catttgcctg agtctacacc actttttgag aacctgaaat
     6961 agaagggaat cttctgtggc ccacagtctc catattggca ctagaagact ggcctggcgg
     7021 aggaatttgc gttggcttgc tttcaggggt tagctacaag attcagcttt atatctctgt
     7081 tgcttcttgg ccagtgtagt caataagggt cttctttaac atctaagata gaggtttggt
     7141 tggccgggcg tggtcgctta ctcctgtaat cccagcactt tgggaggccc agtgaggtgg
     7201 gagaattgct tgaacccagg aggcagaggt tgcagtgagc tgagattgca ccactgcact
     7261 ccagcctggg taacagagtg agactcttgt ctcaaaaaaa aaaaaaaaaa aatctaagat
     7321 agaggtttgg tcaacagtgc ttaataataa ataagaacct cctgccattc taattttcct
     7381 gctgcacccc atcccccaca cacccctcac gaacattgat ataagcagta ttaacacagt
     7441 ataaagaatg ttcaccttgc atatgtcatt tcaggcacat ggattcagga gaagcacagt
     7501 tgagtggaag aaatggtaga cttgtgaggc ttgccccagg ccttgtgtac acgcaataag
     7561 tggtgagcca tgggtctctc cgtcagcgcc tccctccccg ccaccacttc aggccaacaa
     7621 tttaaggtgc tgagttgtaa ggctcctcca ttgtcagtac agggctcgcc tttgtagccc
     7681 tgatcactac cagtacactt ttcaagacaa ctgagtattt ttgtatgcct ttgccttccc
     7741 tttgtccatg aaacatgaag agttgtttat ggttcttgac ttctctgagc agagtgtctg
     7801 catctcttgg agagttacac atttcttcat gagccatttt tctcattctt agatgcacct
     7861 gtttttatcc tttgcagacc atcttctgcc ttcttatttt cctgtctgtc aaagacagaa
     7921 attacaggag atagggaggg ttttttagca tctctttcaa aagatgtatg tcagaatttc
     7981 ctttgcacac caagaactgg agcttagagc cccactattc tctaagccag gttctagtgc
     8041 cttacactcc agaatgtcag atggtgggtg cagattggaa gaaagagaaa agttcatctc
     8101 ggtgtgtggg ttcccatccg ccccacatag cctctccttc ttcggaacaa tgggcgtggg
     8161 gtagaaagct ctttcagtga agggtgttct agcagctcag ttaacacttt actctccagt
     8221 caacacttgg gacatataaa aatgccattg taactactgt agagtcctgt gactcatcgt
     8281 ttgtgtttgt caatttgcag ttcagcttag cccttccctg ttcctgtgta gttacaatct
     8341 ggccctgaag acatccgagg cacttcagta agtgggatct tttctagaga tcctgggtga
     8401 ctttgggtgc acagggtgac cgagcatttc tgcccctgtg aatgtggcac taacactgtg
     8461 cactgtctcc accaagcaag gtttccactg agtttcttct catgttactg ggtttgtaaa
     8521 tgaataaaca cattttaact actcttgca
//
