LOCUS       NM_020786               6997 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pyruvate dehydrogenase phosphatase catalytic subunit 2
            (PDP2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_020786 XM_043826
VERSION     NM_020786.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Zhu,J.J., Huang,F.Y., Chen,H., Zhang,Y.L., Chen,M.H., Wu,R.H.,
            Dai,S.Z., He,G.S., Tan,G.H. and Zheng,W.P.
  TITLE     Autocrine phosphatase PDP2 inhibits ferroptosis by
            dephosphorylating ACSL4 in the Luminal A Breast Cancer
  JOURNAL   PLoS One 19 (3), e0299571 (2024)
   PUBMED   38466744
  REMARK    GeneRIF: Autocrine phosphatase PDP2 inhibits ferroptosis by
            dephosphorylating ACSL4 in the Luminal A Breast Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 6997)
  AUTHORS   Kono,M., Yoshida,N., Maeda,K., Skinner,N.E., Pan,W., Kyttaris,V.C.,
            Tsokos,M.G. and Tsokos,G.C.
  TITLE     Pyruvate dehydrogenase phosphatase catalytic subunit 2 limits Th17
            differentiation
  JOURNAL   Proc Natl Acad Sci U S A 115 (37), 9288-9293 (2018)
   PUBMED   30150402
  REMARK    GeneRIF: ICER was found to bind to the Pdp2 promoter and suppress
            its expression. Furthermore, forced expression of PDP2 in CD4(+)
            cells reduced the in vitro Th17 differentiation, whereas
            shRNA-based suppression of PDP2 expression increased in vitro Th17
            differentiation
REFERENCE   4  (bases 1 to 6997)
  AUTHORS   Kato,J. and Kato,M.
  TITLE     Crystallization and preliminary crystallographic studies of the
            catalytic subunits of human pyruvate dehydrogenase phosphatase
            isoforms 1 and 2
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun 66 (Pt 3), 342-345
            (2010)
   PUBMED   20208177
  REMARK    GeneRIF: catalytic subunit of PDP2 crystals belonged to space group
            P2(1)2(1)2(1), with unit-cell parameters a = 53.6, b = 69.1, c =
            109.7 A.
REFERENCE   5  (bases 1 to 6997)
  AUTHORS   Sugden,M.C. and Holness,M.J.
  TITLE     Recent advances in mechanisms regulating glucose oxidation at the
            level of the pyruvate dehydrogenase complex by PDKs
  JOURNAL   Am J Physiol Endocrinol Metab 284 (5), E855-E862 (2003)
   PUBMED   12676647
  REMARK    Review article
REFERENCE   6  (bases 1 to 6997)
  AUTHORS   Caruso,M., Maitan,M.A., Bifulco,G., Miele,C., Vigliotta,G.,
            Oriente,F., Formisano,P. and Beguinot,F.
  TITLE     Activation and mitochondrial translocation of protein kinase Cdelta
            are necessary for insulin stimulation of pyruvate dehydrogenase
            complex activity in muscle and liver cells
  JOURNAL   J Biol Chem 276 (48), 45088-45097 (2001)
   PUBMED   11577086
REFERENCE   7  (bases 1 to 6997)
  AUTHORS   Huang,B., Gudi,R., Wu,P., Harris,R.A., Hamilton,J. and Popov,K.M.
  TITLE     Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino
            acid sequences, expression, and regulation
  JOURNAL   J Biol Chem 273 (28), 17680-17688 (1998)
   PUBMED   9651365
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292539.1, AC044802.6 and
            BM668681.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_020786.3.
            
            Summary: This gene is a mitochondrial protein that functions as a
            phosphatase and is involved in the enzymatic resetting of the
            pyruvate dehydrogenase complex. Alternative splicing results in
            multiple transcript variants encoding the same protein. [provided
            by RefSeq, Aug 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.84916.1,
                                           SRR1803617.18194.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311765.4/ ENSP00000309548.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2207              AK292539.1         36-2242
            2208-6980           AC044802.6         155655-160427
            6981-6997           BM668681.1         15-31               c
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..6997
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /note="pyruvate dehydrogenase phosphatase catalytic
                     subunit 2"
                     /db_xref="GeneID:57546"
                     /db_xref="HGNC:HGNC:30263"
                     /db_xref="MIM:615499"
     exon            1..126
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /inference="alignment:Splign:2.1.0"
     exon            127..6997
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..1770
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /EC_number="3.1.3.43"
                     /note="protein phosphatase 2C, magnesium-dependent,
                     catalytic subunit 2; protein phosphatase, Mg2+/Mn2+
                     dependent 2B; [Pyruvate dehydrogenase
                     [acetyl-transferring]]-phosphatase 2, mitochondrial;
                     pyruvate dehydrogenase phosphatase isoenzyme 2; pyruvate
                     dehyrogenase phosphatase catalytic subunit 2"
                     /codon_start=1
                     /product="[Pyruvate dehydrogenase
                     [acetyl-transferring]]-phosphatase 2, mitochondrial
                     precursor"
                     /protein_id="NP_065837.1"
                     /db_xref="CCDS:CCDS10822.1"
                     /db_xref="GeneID:57546"
                     /db_xref="HGNC:HGNC:30263"
                     /db_xref="MIM:615499"
                     /translation="MSSTVSYWILNSTRNSIATLQGGRRLYSRYVSNRNKLKWRLFSR
                     VPPTLNSSPCGGFTLCKAYRHTSTEEDDFHLQLSPEQINEVLRAGETTHKILDLESRV
                     PNSVLRFESNQLAANSPVEDRRGVASCLQTNGLMFGIFDGHGGHACAQAVSERLFYYV
                     AVSLMSHQTLEHMEGAMESMKPLLPILHWLKHPGDSIYKDVTSVHLDHLRVYWQELLD
                     LHMEMGLSIEEALMYSFQRLDSDISLEIQAPLEDEVTRNLSLQVAFSGATACMAHVDG
                     IHLHVANAGDCRAILGVQEDNGMWSCLPLTRDHNAWNQAELSRLKREHPESEDRTIIM
                     EDRLLGVLIPCRAFGDVQLKWSKELQRSILERGFNTEALNIYQFTPPHYYTPPYLTAE
                     PEVTYHRLRPQDKFLVLASDGLWDMLSNEDVVRLVVGHLAEADWHKTDLAQRPANLGL
                     MQSLLLQRKASGLHEADQNAATRLIRHAIGNNEYGEMEAERLAAMLTLPEDLARMYRD
                     DITVTVVYFNSESIGAYYKGG"
     regulatory      6969..6974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /note="hexamer: ATTAAA"
     polyA_site      6997
                     /gene="PDP2"
                     /gene_synonym="PDPC 2; PPM2B; PPM2C2"
                     /note="major polyA site"
ORIGIN      
        1 cttctggagc tgggtcctga ctagggaccg cctgggtgag gtgaggacct ggtggccgca
       61 gttgtggcac tgtgcgcagg cgctgaactg accggacgga gcgggcggct gtggcctcgc
      121 cagctggttt aaaaatatcc ttttttgctg aaggaacaca tttgctggta tagtttcaga
      181 atgtcaagta ctgtgtccta ctggatctta aattctacaa ggaacagcat tgccacattg
      241 caagggggta gacgcttata ctccaggtat gtctcaaata ggaataaatt aaaatggagg
      301 ctcttttccc gggtgccacc caccctaaac agttccccat gtggtggctt tactctgtgc
      361 aaagcctaca gacacacatc aacagaggaa gatgattttc acttgcaact cagccctgag
      421 cagataaatg aagtgcttcg agctggcgag acaacccaca agattcttga ccttgaaagc
      481 agagtcccaa attcagtgtt gcggtttgag agcaaccagc tggctgccaa ttccccagtg
      541 gaggaccggc gaggtgtagc ctcctgcctg caaaccaatg gactgatgtt tggcatcttc
      601 gatggacatg gtggtcatgc atgtgcccaa gcagtgagcg agaggctctt ctactatgtg
      661 gcagtgtccc tgatgtccca ccagaccctg gagcacatgg agggagctat ggaaagcatg
      721 aaacccttgc tgcccatcct gcattggctc aagcacccag gggacagtat ctacaaggat
      781 gtcacatctg tgcatcttga ccacctccgt gtctattggc aggaactgct tgatttgcac
      841 atggaaatgg gactaagcat tgaagaagca ttaatgtact ccttccagag actggattct
      901 gacatctcgc tggaaatcca ggcccccctg gaagatgagg tgacaaggaa cctgtcactc
      961 caggttgctt tctctggggc aacagcttgc atggcccatg ttgatggaat tcacttgcac
     1021 gtggcaaatg ctggtgactg ccgagccatc cttggtgtcc aagaggacaa tggcatgtgg
     1081 tcttgtctgc cccttacacg tgaccacaat gcctggaacc aggccgagct gtcccggcta
     1141 aagagggagc accctgagtc agaggacagg acgatcatca tggaggacag gctactgggc
     1201 gtcctcatcc cctgcagggc ctttggggat gttcagctga agtggagtaa agagttgcag
     1261 cgcagcattc tggagagggg cttcaatacc gaggccctca acatttacca gttcacaccc
     1321 ccacactact acactccacc ctacctgact gctgagcctg aggtcacata ccacaggctg
     1381 aggccccagg ataagttcct tgtgctggcc tcagatggcc tgtgggacat gctgagcaat
     1441 gaggacgtgg taaggctggt ggtggggcac ctggctgagg cagattggca caagacagac
     1501 ctggcccaga gacccgccaa cttggggctc atgcagagcc tgctgctgca gaggaaagcc
     1561 agcgggctcc acgaggctga ccaaaatgca gccacgcggc tgatcagaca tgccatcggg
     1621 aacaatgagt atggggagat ggaggcagag cggctggcgg cgatgctgac attgccagag
     1681 gacttggcga ggatgtacag ggatgatatc actgtcactg tggtgtattt taactcagaa
     1741 tcaatcggtg catattacaa ggggggttaa gaatctccca tcctattgtc aaggttaaca
     1801 taaatgctct tctaaaatgt ttcacttact cctaaactag ctatccaaac cttactatta
     1861 aaagcgcagg cagatttaat ttgctaaata gactaacagg aggaaaaaaa caaacagcct
     1921 agctttaaaa aacagtgaaa tagcagtgat ttcatgtccc tgtatgttct gattaagtct
     1981 tatatgcaga gaggacagag cataaagtct atagaattgg cttgggcttg tgtagcccca
     2041 gtcatttgat aaagatgttg ttaaactgtg tcagcctgag ccttcaaagg gtaaatttaa
     2101 tcatgattct gagaataaag aacttcagga gcttcttagg tcttgccaca aaaatctgca
     2161 aatttgataa ttctcctgaa ttcacaatca tggacttgta gactatgaag agatatttta
     2221 tttgtttgtc ttttatttaa attactaagg tctaggttta caaagcatac tacaggaccc
     2281 ttttctatgc aatatcctaa cttttttttt tgtgattatg cttgtgagaa attttgtaat
     2341 actacattct agttctgttt tcgtttattt taaaactgaa tccttaaact tgcaaacacg
     2401 cctactgtaa gcatgcttgg aatgacttgt tttggttttc agctgatagg atctatgcct
     2461 ctggaccagc tgaacttact ggtgcagctt tttgtgccct ctactgagtc ccattccaga
     2521 aactttgagc catgctagga ggtaactagt ctatgcctta actcctgacc tttcctgcat
     2581 tagtaaaatt tcctcctggg ctgagccttt gggggttgat tcacagcatt taagctttct
     2641 gattatacct ctcaaacatt cttctgtcaa ttccacacaa tccttctacc tctgctatgc
     2701 tgaacccctc attcaccctg cacctgccaa ctgcaaaata ttgtctgaaa gaatctttca
     2761 catgttcctg cttcctatgg ctgcaggaaa ggagaaaatg tgattgcatg tggtagttgt
     2821 ggtgatagac gtttagacag gacaggttaa tttagcggat tgtggactta agattcaacc
     2881 tctatctgga gaggtgaaaa cacaaaggct taaggacaac agtttctaac caagtttctc
     2941 tgaatccttt cctgagaggt ggtgggcagg agggaggagt gagacttatc agatgggacg
     3001 cttgccccaa gactgtggga taaaccacca ttgctagtcc tcaggaatta gaccatattt
     3061 agtataggag ttggggcaaa aataaaagtg taatttgtaa tggtttctgt gggctgtaga
     3121 ctctttgtat ttacttggaa attcagttat gtggatccat tttggagatg aatttcatct
     3181 catgagttgc tgtggcattt aaacaacagg aaatgtgtcc ctaaatggaa acaaatataa
     3241 tcccacaaaa ccaacaattt tatttgcctc tactacgtca ggcagaattc ttcaattaag
     3301 ttgaactaca tttgttgatc ccttttctca tcttacccct ttatgccacc tactgaaaag
     3361 aaaaacaaat agctaggttt ggtttcctgt tttttgtcta tgtcccagct gacattcagt
     3421 caaatgttgg gatacttcta atttggtggt gtttctttgt acatttttca gtgcaactgt
     3481 ttagtcatgt gttttcatct aagaaattat cttttgaata tttaagcctt gatcctttta
     3541 ttctgaaacc attaatattc cttcttggct gggtgcagta gtttacgcct gtaatcccag
     3601 cactgtggga ggccgaggcg gatggatcac ctgaggtcgg gaattcgaga ccagcctgac
     3661 caacatggag aaactccatc tctactaaaa atacaaaatt agccaggtgt ggtggtgcat
     3721 gcctgtaatc ccagctactc gggaggctga ggcaggaaaa tcacttgaac ccgggaggcg
     3781 gaggttgcgg tgagccaaga tcgcaccatt gcactccagc ctgggcaaca agagtgaaac
     3841 tctgtctcaa aaaaaaaaaa aaaaaatcca tcttatctct tagtccgtcc ttccttcctt
     3901 ccttccttcc ttccttcctt ccttccttcc ttccttcctt ccttccttcc ttcctctctc
     3961 tctctttctt tctttctttc cttcttgaca gggtctccct gtagccaggc tggagtgcaa
     4021 tggcatgatc ttggctcact gcaacctctg cagcccaggc tcaaacgatc ctcctgcctc
     4081 agcctcccga gtagctggga ctatgcacca ccatgcctgg ctaatttttt taattttttg
     4141 tagagacagg gttttgccat gttgcccagg ctggtctcaa actcctagac tcaagcaatc
     4201 tgcccacctc ggcctcccaa ggtgctggga tgataggcat gagccaccgc acccagccta
     4261 tctcctagat cttgaatcaa aaataaaatg taaatcactt ccattctgct agcatgaggg
     4321 tttgttgcta aaattgagga ttctatctga cgtttacttt ttgttgtttt ttgagacaag
     4381 gtcttgcttt gtcgcccatg ctggagtgca gtggcatcat tataactcac tgtcaaactc
     4441 ctggactcca atgttccctc cccttttagc ctcccaagta gctgggtcta caggcatgaa
     4501 ccatcaggcc cagataatta aaaaaacttt ttttggtgaa aacatgatct tgctatgttg
     4561 ctcaggctgg ttgtctggca ttttctttgt cctttattct ttatactagc taaaccctgg
     4621 agtatccttg aagcctttac tggcatacag agtacctgaa tcttactttg gagcctagca
     4681 tgggtcataa atatatctga ttcattaacc tgtgagacag ctgagagaac acagactcct
     4741 tacctgtatc tccaggcata tctggttttt ctctgcctaa tagattttcc tgctttctag
     4801 ttagtaatat tgagaaagaa gaaaggctta tttatcattt gctttttttc cactgtgaat
     4861 catctgttct ttccatgaac attctgggga gcacctcctg attaaactcc tgtctccctg
     4921 accactatcc tgctgtcatt ggacttcttg aagcacatgg gctactgccc caggacactg
     4981 ggatttgaga ggagtttagt ggtaattgtc aggtctttca aagcatttat tattcctttt
     5041 atacggagat ttcagtattg agacttaaaa tgaactgaaa aatgagattg aacatttaat
     5101 attttggatg taacttttga agaaagtatg ctttggtgct taaaattgta tatgatttta
     5161 ggtaagaaac tttgataata ttggcataat ttagatttat tttctttctt ttttttgaga
     5221 cagtctcact cagtcgccca ggctgaagtg cagtgacaca gtctcagctc actgcaacgt
     5281 ctgcctccca gattgaagtg actctcgtgc ctctgccaca gagtagctgg gattacaggc
     5341 atgcaccacc acacaccgct aattttttgt atttttagta gagacagagt ttcaccatgt
     5401 tggccaggct gcgaactcct gagctcaagt gatcctccca cctcagcttc ccaaagtgct
     5461 agcattacag gcatgagcca ccacacctca ccagattttt aaaaaatata taactgcatc
     5521 tctcttgatt ctggggcttg gtaaaaatgg atagataaga tagtattcta aattcaaatt
     5581 cgtggctagg cacagtggcc cacacctgta atcccagcac tttgggattc caagacagaa
     5641 gactcacttg agtacagtat gagaccagcc tgggcaacat agatcttgcc tctacaaaaa
     5701 aaaaaaaaaa tagccaggtg tggcacatgc ctgtagtctc agctgcttgg aaggctgaaa
     5761 tgagaggatc tcttgagccc aggaggtcta ggccagagtg agctgtgatc gtgccattgc
     5821 actccagact gagtgacaga gtgagactgt gtctaaaaaa aaagtttgaa ttaaaaaaaa
     5881 aaaaaaaaaa gtcggctgtg cacggtggct catgcctgtg atcccagcac tttcggaggc
     5941 caaagtgggt ggattacctg aggtcaggag tttgagacca gcctgaccaa catggtaaaa
     6001 ctccatctct actaaaaata caaaaattag cctggaatag tggcgcatgc ctgtaatccc
     6061 agctacttgt gaggctgagg caggagaatc ccttgaaccc agaaggcaga ggttgcagtg
     6121 agctgagatt gtgccattgc actccagcct gggcaacatg agcgaaactc catctcaaaa
     6181 aaacaaaaac aaaaaacaaa ctcaaattag catgatagat caggctactt agattcagct
     6241 ttctgagatg ttatttgtta acattaaggc taatttatta taatgaaaat gtaaactttg
     6301 aggtatgtta atatataaac atctttttcc tttggcctaa aaggtcttta gtattcagca
     6361 cactgggtaa agttcagttt agacacaatc aaattggcat cttttaaaca taagtgaaat
     6421 atagagagct tgaacttgag ttacttaaag aagatacagt ataattaatt atacagaaca
     6481 aaacaaagat gtgtttagag tgactctggc ctgactcttg tccatcagcc cctagtagcc
     6541 tatcttcagc ctttgggaag tcttaacttc ccaggttaaa ctagaatatt ctggaactga
     6601 gctgtccaaa tttcatctta aagtggatca tttgactctg aaacacgatt ctcatggcag
     6661 aaaaagccct ggtgtttcct tggagctggg gataaaggcc agtgagccag cctgtgatga
     6721 gagctgcccc tggcactatc tgagtggaaa gctcattggg acagccatgc agctatcaga
     6781 gccgactgaa gccctgtccc ttacccctca ggcacctgat gcttaacttg cctcctggga
     6841 aataatgatc tagcatttac ttggaacaat gattaatcat gttctttaat aattgctgac
     6901 atttgtattt taagctgtat accaatgttc gtatttgaca ttgattttct aaccattaga
     6961 gatatttaat taaaacttgg aaatgaaaga aacaaaa
//
