LOCUS       NM_002611               3364 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript
            variant 1, mRNA.
ACCESSION   NM_002611 XR_109402 XR_111716 XR_115120
VERSION     NM_002611.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Kitamura,S., Yamaguchi,K., Murakami,R., Furutake,Y., Higasa,K.,
            Abiko,K., Hamanishi,J., Baba,T., Matsumura,N. and Mandai,M.
  TITLE     PDK2 leads to cisplatin resistance through suppression of
            mitochondrial function in ovarian clear cell carcinoma
  JOURNAL   Cancer Sci 112 (11), 4627-4640 (2021)
   PUBMED   34464482
  REMARK    GeneRIF: PDK2 leads to cisplatin resistance through suppression of
            mitochondrial function in ovarian clear cell carcinoma.
REFERENCE   3  (bases 1 to 3364)
  AUTHORS   Gong,J., Du,C., Sun,N., Xiao,X. and Wu,H.
  TITLE     Circular RNA hsa_circ_0005397 promotes hepatocellular carcinoma
            progression by regulating the miR-326/PDK2 axis
  JOURNAL   J Gene Med 23 (6), e3332 (2021)
   PUBMED   33783904
  REMARK    GeneRIF: Circular RNA hsa_circ_0005397 promotes hepatocellular
            carcinoma progression by regulating the miR-326/PDK2 axis.
REFERENCE   4  (bases 1 to 3364)
  AUTHORS   Chen,H.L., Cui,B.M., Kang,Y.Z., Wang,L.W., Wang,J.N., Feng,Y.,
            Zhang,P., Lu,D. and Chen,J.
  TITLE     [Expression Level of Protein Kinase D in Oral Squamous Cell
            Carcinoma with Diverse Differentiation]
  JOURNAL   Sichuan Da Xue Xue Bao Yi Xue Ban 51 (6), 755-759 (2020)
   PUBMED   33236596
  REMARK    GeneRIF: [Expression Level of Protein Kinase D in Oral Squamous
            Cell Carcinoma with Diverse Differentiation].
REFERENCE   5  (bases 1 to 3364)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3364)
  AUTHORS   Steussy,C.N., Popov,K.M., Bowker-Kinley,M.M., Sloan,R.B. Jr.,
            Harris,R.A. and Hamilton,J.A.
  TITLE     Structure of pyruvate dehydrogenase kinase. Novel folding pattern
            for a serine protein kinase
  JOURNAL   J Biol Chem 276 (40), 37443-37450 (2001)
   PUBMED   11483605
REFERENCE   7  (bases 1 to 3364)
  AUTHORS   Baker,J.C., Yan,X., Peng,T., Kasten,S. and Roche,T.E.
  TITLE     Marked differences between two isoforms of human pyruvate
            dehydrogenase kinase
  JOURNAL   J Biol Chem 275 (21), 15773-15781 (2000)
   PUBMED   10748134
REFERENCE   8  (bases 1 to 3364)
  AUTHORS   Gold,M.R., Scheid,M.P., Santos,L., Dang-Lawson,M., Roth,R.A.,
            Matsuuchi,L., Duronio,V. and Krebs,D.L.
  TITLE     The B cell antigen receptor activates the Akt (protein kinase
            B)/glycogen synthase kinase-3 signaling pathway via
            phosphatidylinositol 3-kinase
  JOURNAL   J Immunol 163 (4), 1894-1905 (1999)
   PUBMED   10438924
REFERENCE   9  (bases 1 to 3364)
  AUTHORS   Kobayashi,T. and Cohen,P.
  TITLE     Activation of serum- and glucocorticoid-regulated protein kinase by
            agonists that activate phosphatidylinositide 3-kinase is mediated
            by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
  JOURNAL   Biochem J 339 (Pt 2) (Pt 2), 319-328 (1999)
   PUBMED   10191262
REFERENCE   10 (bases 1 to 3364)
  AUTHORS   Gudi,R., Bowker-Kinley,M.M., Kedishvili,N.Y., Zhao,Y. and
            Popov,K.M.
  TITLE     Diversity of the pyruvate dehydrogenase kinase gene family in
            humans
  JOURNAL   J Biol Chem 270 (48), 28989-28994 (1995)
   PUBMED   7499431
  REMARK    Erratum:[J Biol Chem 1996 Jan 12;271(2):1250]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002401.2, BC040478.1 and
            BQ024783.1.
            
            On May 17, 2019 this sequence version replaced NM_002611.4.
            
            Summary: This gene encodes a member of the pyruvate dehydrogenase
            kinase family. The encoded protein phosphorylates pyruvate
            dehydrogenase, down-regulating the activity of the mitochondrial
            pyruvate dehydrogenase complex. Overexpression of this gene may
            play a role in both cancer and diabetes. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.21464.1,
                                           SRR1803613.61486.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000503176.6/
                                                  ENSP00000420927.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AC002401.2         60133-60197         c
            66-1731             BC040478.1         85-1750
            1732-1732           AC002401.2         45028-45028         c
            1733-2271           BC040478.1         1752-2290
            2272-2581           BQ024783.1         18-327              c
            2582-3364           AC002401.2         43396-44178         c
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..3364
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /note="pyruvate dehydrogenase kinase 2"
                     /db_xref="GeneID:5164"
                     /db_xref="HGNC:HGNC:8810"
                     /db_xref="MIM:602525"
     exon            1..203
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1309
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /EC_number="2.7.11.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; pyruvate dehydrogenase kinase, isoenzyme 2; PDH
                     kinase 2; [Pyruvate dehydrogenase (acetyl-transferring)]
                     kinase isozyme 2, mitochondrial; pyruvate dehydrogenase
                     kinase, isozyme 2; pyruvate dehydrogenase, lipoamide,
                     kinase isozyme 2, mitochondrial"
                     /codon_start=1
                     /product="pyruvate dehydrogenase kinase, isozyme 2 isoform
                     1 precursor"
                     /protein_id="NP_002602.2"
                     /db_xref="CCDS:CCDS11559.1"
                     /db_xref="GeneID:5164"
                     /db_xref="HGNC:HGNC:8810"
                     /db_xref="MIM:602525"
                     /translation="MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNA
                     CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD
                     PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYL
                     SRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMA
                     SPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM
                     VALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISR
                     LYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWC
                     VPSTEPKNTSTYRVS"
     exon            204..345
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            346..417
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            418..602
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            603..692
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            693..770
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            771..847
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            848..946
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1054
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1168
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..3364
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2249..2254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /note="hexamer: AGTAAA"
     polyA_site      2271
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /note="major polyA site"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
                     /note="hexamer: AATAAA"
     polyA_site      2581
                     /gene="PDK2"
                     /gene_synonym="PDHK2; PDKII"
ORIGIN      
        1 ggcggccgaa ccgcgtcgct gggccgaaag gtgcgcgagc gctgcccgcg cggggaccac
       61 aaccaaagtc gcggccgccg cagccatgcg ctgggtgtgg gcgctgctga agaatgcgtc
      121 cctggcaggg gcgcccaagt acatagagca cttcagcaag ttctccccgt ccccgctgtc
      181 catgaagcag tttctggact tcggatccag caatgcctgt gagaaaacct ccttcacctt
      241 cctcaggcag gagctgcctg tgcgcctggc caacatcatg aaagagatca acctgcttcc
      301 cgaccgagtg ctgagcacac cctccgtgca gctggtgcag agctggtatg tccagagcct
      361 cctggacatc atggagttcc tggacaagga tcccgaggac catcgcaccc tgagccagtt
      421 cactgacgcc ctggtcacca tccggaaccg gcacaacgac gtggtgccca ccatggcaca
      481 aggcgtgctt gagtacaagg acacctacgg cgatgacccc gtctccaacc agaacatcca
      541 gtacttcctg gaccgcttct acctcagccg catctccatc cgcatgctca tcaaccagca
      601 caccctcatc tttgatggca gcaccaaccc agcccatccc aaacacatcg gcagcatcga
      661 ccccaactgc aacgtctctg aggtggtcaa agatgcctac gacatggcta agctcctgtg
      721 tgacaagtat tacatggcct cacctgacct ggagatccag gagatcaatg cagccaactc
      781 caaacagccg attcacatgg tctacgtccc ctcccacctc taccacatgc tctttgagct
      841 cttcaagaat gccatgaggg cgactgtgga aagccatgag tccagcctca ttctcccacc
      901 catcaaggtc atggtggcct tgggtgagga agatctgtcc atcaagatga gtgaccgagg
      961 tgggggtgtt cccttgagga agattgagcg actcttcagc tacatgtact ccacagcacc
     1021 caccccccag cctggcaccg ggggaacgcc gctggctggc tttggttatg ggctccccat
     1081 ttcccgcctc tacgccaagt acttccaggg agacctgcag ctcttctcca tggaaggctt
     1141 tgggaccgat gctgtcatct atctcaaggc cctgtccacg gactcggtgg agcgcctgcc
     1201 tgtctacaac aagtcagcct ggcgccacta ccagaccatc caggaggccg gcgactggtg
     1261 tgtgcccagc acggagccca agaacacgtc cacgtaccgc gtcagctaag ggccgccgtg
     1321 catctgcacc tgagaggacg gactgccgcc tctgggtccc cccaccgtgg tgcccctcac
     1381 catcctcctg ggggagcagg gggtgggttc tccctgatga ccaggttctg tctctatgga
     1441 agtcactgcg gtgataggtc tgtgatggtc cctaagtgcc agtccatctc tgtggagacc
     1501 cctcggtggc ctccctatct ctgtgggcga tgcctgaggg ttagggatgt ctccaccctg
     1561 atggggtgtc ccagagacat tttcccatgg cagtcctcct ctctgagacc agggctgtca
     1621 cttttctgcc aggggtactg ggtccccctc agcaccctcc acagcacagg ccttccaagt
     1681 ggatgtcccg ttgccttatt cccccagccc acaaaggcac cctggccttg gcctgctgaa
     1741 gtgttaggaa gagggtgggt gccctccaga cctggggact gagtggggaa aggagttaca
     1801 cccgtgagtg gggaatgagg ctggtcctgc agcctctccc tccgctcagg gcttgaaggt
     1861 cggtggcgga gggggtggct ctcacagggc ccaactctaa agtggaagaa ccttgttaga
     1921 ccgagagctt gccatccagc caagctgctc gaggccctgc agtggccttg gcaatgtctg
     1981 tgccacctcc tgagccctcc cagcatgtcc tcacatgctc atgcccaccc gctcctccac
     2041 aagcctagtc catcctgcct gagctccagc ccccagcccc cactgtgccc agacatgtgt
     2101 gctcagggtg gctttctccc taggaccttc tgtgtatata gttagtttta taaccctgaa
     2161 tgcccccacc cttcccctaa gcacacaggg gttaaagctg tgtgtccctc ccagtggctg
     2221 tggcagtgac agtgacaccc acacccacag taaagaggag actgaatgag actggcctgg
     2281 ctgcatccct gggggagggg acccacacgt gcgcacgtac acacgcacac tgcggtgccc
     2341 tgtgaccacc acatgacgca ccggggacgt ccggcccagc tcaggactgt gagtaaaaca
     2401 ctgggcaaag ccatctgttc agaacgccgg atggcccagc acctggtgac agacctgttg
     2461 tcccaccata ctcagggccc agcagagtcc ggccaaccca acaactggag cagacagaaa
     2521 ttctggtcac tggcacatca taaggattta ttgaaataaa ttgagaactg cctccccttc
     2581 acatgcaggt gggcacccac tatagctact gatggcttta aggatgtggg tgccttccaa
     2641 cctcccatgg ccctgccacc aggcccagat ggtgccagtg tcctcatatc tgtttccctc
     2701 cgaagcccct ctcccagcac agatagcaga ggggggataa gggctgagtc ccaggtacag
     2761 ggtgctggcc gtaagccaca gcccctgggc aggaatgagg gagccagcat gcattggtgc
     2821 catctgccgc tctgtctggg cagatgggag gagctggagg ggtttccaag cgggagcccc
     2881 caggcctcat cggtaactct gaccagtgtc ctggaagcgg gactagctgc tgaccagcgc
     2941 cacacatgaa cacttcctcc aaggacattt cctaaaaagg acacatgctg tccccaggct
     3001 gctggtaaac tccaccccca tccagggagc tggccccagc cctcagtgtg gcccagggaa
     3061 gcagagcttg caccgcctgc cagttctgag cccactgatt ccctgacaat gcctggggca
     3121 gatgggggag ggctgatgaa cctttccaat ctcgtagctg ccggggaccc ccaggttttg
     3181 cctctctgcc tggctggggt gagggctgac caggtcagca gctcctaggc cttggctggt
     3241 ggaagtggtg tcctctgtcc ctatcagttc ctgtttgctc agctcccaaa gaggacttag
     3301 gtgccctctg aggaagcagc aggaagtgaa gggagtcctg gcttggagaa tgggtcaaag
     3361 acca
//
