LOCUS       NM_002610              14072 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens pyruvate dehydrogenase kinase 1 (PDK1), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_002610
VERSION     NM_002610.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Kociper,B., Skorja Milic,N., Ogrizek,I., Mis,K. and Pirkmajer,S.
  TITLE     Inhibition of the ubiquitin-proteasome system reduces the abundance
            of pyruvate dehydrogenase kinase 1 in cultured myotubes
  JOURNAL   J Muscle Res Cell Motil 45 (3), 155-169 (2024)
   PUBMED   39080182
  REMARK    GeneRIF: Inhibition of the ubiquitin-proteasome system reduces the
            abundance of pyruvate dehydrogenase kinase 1 in cultured myotubes.
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   Su,M., Liang,Z., Shan,S., Gao,Y., He,L., Liu,X., Wang,A., Wang,H.
            and Cai,H.
  TITLE     Long non-coding RNA NEAT1 promotes aerobic glycolysis and
            progression of cervical cancer through WNT/beta-catenin/PDK1 axis
  JOURNAL   Cancer Med 13 (9), e7221 (2024)
   PUBMED   38733179
  REMARK    GeneRIF: Long non-coding RNA NEAT1 promotes aerobic glycolysis and
            progression of cervical cancer through WNT/beta-catenin/PDK1 axis.
REFERENCE   3  (bases 1 to 14072)
  AUTHORS   Zhu,G., Guan,F., Li,S., Zhang,Q., Zhang,X., Qin,Y., Sun,Z.,
            Peng,S., Cheng,J., Li,Y., Ren,R., Fan,T. and Liu,H.
  TITLE     Glutaminase potentiates the glycolysis in esophageal squamous cell
            carcinoma by interacting with PDK1
  JOURNAL   Mol Carcinog 63 (5), 897-911 (2024)
   PUBMED   38353358
  REMARK    GeneRIF: Glutaminase potentiates the glycolysis in esophageal
            squamous cell carcinoma by interacting with PDK1.
REFERENCE   4  (bases 1 to 14072)
  AUTHORS   Wu,K., Wu,B., Yan,K., Ding,Q. and Miao,Z.
  TITLE     KLK10 promotes the progression of KRAS mutant colorectal cancer via
            PAR1-PDK1-AKT signaling pathway
  JOURNAL   Cell Biol Int 48 (4), 440-449 (2024)
   PUBMED   38115179
REFERENCE   5  (bases 1 to 14072)
  AUTHORS   Chiou,Y.Y., Lee,C.Y., Yang,H.W., Cheng,W.C. and Ji,K.D.
  TITLE     Circadian modulation of glucose utilization via CRY1-mediated
            repression of Pdk1 expression
  JOURNAL   J Biol Chem 300 (2), 105637 (2024)
   PUBMED   38199564
  REMARK    GeneRIF: Circadian modulation of glucose utilization via
            CRY1-mediated repression of Pdk1 expression.
REFERENCE   6  (bases 1 to 14072)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Site specificity of four pyruvate dehydrogenase kinase isoenzymes
            toward the three phosphorylation sites of human pyruvate
            dehydrogenase
  JOURNAL   J Biol Chem 276 (40), 37223-37229 (2001)
   PUBMED   11486000
REFERENCE   7  (bases 1 to 14072)
  AUTHORS   Kolobova,E., Tuganova,A., Boulatnikov,I. and Popov,K.M.
  TITLE     Regulation of pyruvate dehydrogenase activity through
            phosphorylation at multiple sites
  JOURNAL   Biochem J 358 (Pt 1), 69-77 (2001)
   PUBMED   11485553
REFERENCE   8  (bases 1 to 14072)
  AUTHORS   Gudi,R., Bowker-Kinley,M.M., Kedishvili,N.Y., Zhao,Y. and
            Popov,K.M.
  TITLE     Diversity of the pyruvate dehydrogenase kinase gene family in
            humans
  JOURNAL   J Biol Chem 270 (48), 28989-28994 (1995)
   PUBMED   7499431
  REMARK    Erratum:[J Biol Chem 1996 Jan 12;271(2):1250]
REFERENCE   9  (bases 1 to 14072)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Mutagenesis studies of the phosphorylation sites of recombinant
            human pyruvate dehydrogenase. Site-specific regulation
  JOURNAL   J Biol Chem 270 (24), 14297-14304 (1995)
   PUBMED   7782287
REFERENCE   10 (bases 1 to 14072)
  AUTHORS   Liu,S., Baker,J.C., Andrews,P.C. and Roche,T.E.
  TITLE     Recombinant expression and evaluation of the lipoyl domains of the
            dihydrolipoyl acetyltransferase component of the human pyruvate
            dehydrogenase complex
  JOURNAL   Arch Biochem Biophys 316 (2), 926-940 (1995)
   PUBMED   7864652
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK312700.1, AC018712.5 and
            BQ023252.1.
            
            On Jun 3, 2019 this sequence version replaced NM_002610.4.
            
            Summary: Pyruvate dehydrogenase (PDH) is a mitochondrial
            multienzyme complex that catalyzes the oxidative decarboxylation of
            pyruvate and is one of the major enzymes responsible for the
            regulation of homeostasis of carbohydrate fuels in mammals. The
            enzymatic activity is regulated by a
            phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by
            a specific pyruvate dehydrogenase kinase (PDK) results in
            inactivation. Multiple alternatively spliced transcript variants
            have been found for this gene. [provided by RefSeq, Jun 2013].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, compared to variant 1. The resulting isoform (2)
            lacks an internal segment, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.133463.1,
                                           SRR1803615.57471.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000282077.8/
                                                  ENSP00000282077.3
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1372              AK312700.1         1-1372
            1373-4106           AC018712.5         153909-156642       c
            4107-4537           BQ023252.1         17-447              c
            4538-14072          AC018712.5         143943-153477       c
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..14072
                     /gene="PDK1"
                     /note="pyruvate dehydrogenase kinase 1"
                     /db_xref="GeneID:5163"
                     /db_xref="HGNC:HGNC:8809"
                     /db_xref="MIM:602524"
     exon            1..257
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1372
                     /gene="PDK1"
                     /EC_number="2.7.11.2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; pyruvate dehydrogenase kinase, isoenzyme 1;
                     mitochondrial pyruvate dehydrogenase, lipoamide, kinase
                     isoenzyme 1; PDH kinase 1; [Pyruvate dehydrogenase
                     (acetyl-transferring)] kinase isozyme 1, mitochondrial"
                     /codon_start=1
                     /product="[Pyruvate dehydrogenase (acetyl-transferring)]
                     kinase isozyme 1, mitochondrial isoform 2 precursor"
                     /protein_id="NP_002601.1"
                     /db_xref="CCDS:CCDS2250.1"
                     /db_xref="GeneID:5163"
                     /db_xref="HGNC:HGNC:8809"
                     /db_xref="MIM:602524"
                     /translation="MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPG
                     QVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLL
                     RTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGV
                     IEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGS
                     INPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHM
                     VFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNY
                     MYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKAL
                     STDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA"
     exon            258..399
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            400..471
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            472..656
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            657..752
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            753..830
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..907
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1006
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1117
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1231
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..14072
                     /gene="PDK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4167..4172
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDK1"
                     /note="hexamer: AATAAA"
     polyA_site      4189
                     /gene="PDK1"
                     /note="major polyA site"
     regulatory      4203..4208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDK1"
                     /note="hexamer: AATAAA"
     polyA_site      4233
                     /gene="PDK1"
                     /note="major polyA site"
     regulatory      14047..14052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDK1"
                     /note="hexamer: ATTAAA"
     polyA_site      14072
                     /gene="PDK1"
ORIGIN      
        1 actcggcaga ggcgcgggga aacctggcgt actggctgtg gcttctctag cgggactcgg
       61 catgaggctg gcgcggctgc ttcgcggagc cgccttggcc ggcccgggcc cggggctgcg
      121 cgccgccggc ttcagccgca gcttcagctc ggactcgggc tccagcccgg cgtccgagcg
      181 cggcgttccg ggccaggtgg acttctacgc gcgcttctcg ccgtccccgc tctccatgaa
      241 gcagttcctg gacttcggat cagtgaatgc ttgtgaaaag acctcattta tgtttctgcg
      301 gcaagagttg cctgtcagac tggcaaatat aatgaaagaa ataagtctcc ttccagataa
      361 tcttctcagg acaccatccg ttcaattggt acaaagctgg tatatccaga gtcttcagga
      421 gcttcttgat tttaaggaca aaagtgctga ggatgctaaa gctatttatg actttacaga
      481 tactgtgata cggatcagaa accgacacaa tgatgtcatt cccacaatgg cccagggtgt
      541 gattgaatac aaggagagct ttggggtgga tcctgtcacc agccagaatg ttcagtactt
      601 tttggatcga ttctacatga gtcgcatttc aattagaatg ttactcaatc agcactcttt
      661 attgtttggt ggaaaaggca aaggaagtcc atctcatcga aaacacattg gaagcataaa
      721 tccaaactgc aatgtacttg aagttattaa agatggctat gaaaatgcta ggcgtctgtg
      781 tgatttgtat tatattaact ctcccgaact agaacttgaa gaactaaatg caaaatcacc
      841 aggacagcca atacaagtgg tttatgtacc atcccatctc tatcacatgg tgtttgaact
      901 tttcaagaat gcaatgagag ccactatgga acaccatgcc aacagaggtg tttacccccc
      961 tattcaagtt catgtcacgc tgggtaatga ggatttgact gtgaagatga gtgaccgagg
     1021 aggtggcgtt cctttgagga aaattgacag acttttcaac tacatgtatt caactgcacc
     1081 aagacctcgt gttgagacct cccgcgcagt gcctctggct ggttttggtt atggattgcc
     1141 catatcacgt ctttacgcac aatacttcca aggagacctg aagctgtatt ccctagaggg
     1201 ttacgggaca gatgcagtta tctacattaa ggctctgtca acagactcaa tagaaagact
     1261 cccagtgtat aacaaagctg cctggaagca ttacaacacc aaccacgagg ctgatgactg
     1321 gtgcgtcccc agcagagaac ccaaagacat gacgacgttc cgcagtgcct agacacactt
     1381 gggacatcgg aaaatccaaa tgtggctttt gtattaaatt tggaaggtat ggtgttcaga
     1441 actatattat accaagtact ttatttatcg ttttcacaaa actatttgag tagaataaat
     1501 ggaaactgaa ttccatttgt gcccgttaaa cctcctaaag gatgaaattg cacctatttt
     1561 acacttatat tttcacagtt aattgaacat atttttaaac aactgtagtt ttgggcaact
     1621 tttcactttg tggtagactt cagaagtgtg gaaatcttcg ggtttctata ggaaactagt
     1681 tttttttttt taagaaatac tttcatttat gtttgctaga aacattttct aaatgatagt
     1741 gattagctgg ttagccatct tcctgttatt tggaggagtt ctgccatctt ttattcggta
     1801 gtgacagctg taatatggtc ttcatgttta tcagtgactt gactacaagt aaagcagaaa
     1861 acagttggcc tgtaatcaca gaaccagcca cccttcggcg tctacatgca ttgtcttagc
     1921 tctgaggtta atttaccatt tttaaatttt attgtaaaga agtgtaatga ctagctctct
     1981 gtggatctat gccaaaatca tgggccatct tttctaagtg tacttctgat taatgcaagg
     2041 ggaaattttt atactgaaaa caaacaaaca aactagcagc tactaaaata gagaagaaat
     2101 tgagggcaaa gtaatatgtc tccaaaactg aactgattgc ttgttgagat tacctagaaa
     2161 gcttttggaa aaaaatagag cttttctgca tcctactcca gatacactga atcggaatcc
     2221 ctagggctag agcctagaaa tctcactttt taaaatgctt ccaaggtaag tctgatgcct
     2281 accctaggtt gggaaccact ctttcaaagt aaacagtgat gacacagcat ttgctaaagt
     2341 aacatctgta ggttttggtt tccacctatt taaagtcaga tttatagact cgaggagaca
     2401 gaaactgaat cccacacctg gtgcaattag aagcataatt agaagcagaa gaacaaaatg
     2461 ccacgtaacc aaagcatcag agccatcatt gcacgtgtct ttttttcttt tttctttttt
     2521 ttttttcaag acagggtctc gctcttttgc ccaggatgga gtacagtggc atggtcaagg
     2581 ctcactgcag cttcaatctt ctgggctcaa gtaatccttc cacctcagcc tcccatatag
     2641 ctggaactac agttgtgcac taccacaccc agctaatttt ttgatttctt gtggagatga
     2701 ggtctcagta tgttcccagg ctagtgttga actcctgagc tcaagaaatc ctcctgcctg
     2761 agtctctgaa agtgctggga ttacaggcgt gagccaccac acatggccta ttgcacaagt
     2821 cttgacaaac aacatatatt ttctaaaact atccagattt cattatcagg gaaaagtttg
     2881 cttaggttta ttacagcagt ccttggcatt tgtatggtaa tttgtacttt acaaatactt
     2941 tgatatatta taactctatt ggtctttgaa acaaagcgat acagtgtagg tggtatcatt
     3001 ctctttctca ctcagtgtgg cccagagttg ctcagaattg gagcagagcc tgagacgtat
     3061 ctgcagatcc tgtcatcagc tggcaagtcc aggagactgt gtcatttaga gactgtgttg
     3121 ttagttatcc ctcaacatct tctaaggtgg caggaaataa tattggaaat aacattttaa
     3181 agtaaaaatt ttaaagttta aagaagagtt ttgccactta aacaggggag ctttgtctgg
     3241 aaaatacact gagttgaaac acttcatcct tggaaggatt atataagatg aacagttgtg
     3301 ataaatgtgt agattagagg gatgtgaatg ggcagttagt ccagtgccct catttaagag
     3361 gccaagatcc tgattcagag gaggcatcct ttgcccagag ctgcttagct aatctgacca
     3421 aatgttggga aaaatgtctc acctaaccca ctattcctta attatggatt ttgtgaaaaa
     3481 caatagaaca tgttaatgag taatttatat tagttcgatg tattacaatt ttttagcttt
     3541 aaattacagt tttcttataa tgttgaaatg ttttagaatc ctttgaatct aagtatttgt
     3601 ttcctaaatg aaacatttgt acaacatttg atgtttttac ttatgaaata ttctcctccc
     3661 ccaagaaaat ttaaactttt tctctctatt taaaagctaa gaaatgtttt aaaggaaaaa
     3721 tgaaattatc ttcctttagc ttatttttaa ggtaaaacag ctttttactc tgttattgtg
     3781 gtaatggaca gaatattaca tacaaaaata ttctgggaga gctttttcct agttggtttt
     3841 aaatcattgt gccacctgaa aggtttttag attttatagg agctaatttg tccaccagca
     3901 ttaatgtaac acagtgtagt tatgaaaata tattgaagga caggaagtgg acacgaagtg
     3961 atttttgtaa cctgagcagt taatgaatgt gccaacattt tctaggaagg gacagcaaga
     4021 atattctgct ctgtagttaa aatactggct ggcttttgat gtcttcatgc ttaattgtga
     4081 tcactttctt gcactgtgat gtttttacgt gaatatgttg aagtagaagt ctaccatatt
     4141 attttataaa atgttttctg tatggcaata aactgaaaac atggatcaac ccttcttttg
     4201 aaaataaact gagtcaattt agccttttaa aaatatagtc atctctttta aatagaatcc
     4261 tcttccacca tcaaggctca acattttgta agcatccaaa aaattggtaa ttagggggct
     4321 tgcactaaat ttcactatct tcagtagaga ggaactgttt ggaacttaga tttccaatgt
     4381 gtatattcta atggagaaag caagaggtag agtttgtatg tttgacttac cttagatttt
     4441 tattttccat acatactgca aatgattgac ttgttgcata aatgaagatc ttctgttgtg
     4501 tgcttttcaa acactgtaaa taaatttgaa atttgaataa ctttccacag tataactgta
     4561 aaaaaaaaaa aaaaaagtct taatttaacc agcattaccc tctgcttgta ccaattgaat
     4621 ggcagaacta ctatagtcaa aaataaattc ctatcctatt tttaattgat gggatatagg
     4681 cagcacaatt tcccttcatt gttgaagcca acaagttccg taacagaact ccagaggtaa
     4741 atagctggga aaactgttag ttttaaggtc ccactgttgc tctgtgaatg actaatcaga
     4801 ggaagccaaa aatcagccaa ctaatttcag tttatgtctt taattgtgat gtaagtgtga
     4861 agtttctaca tatgggtaat cagggttgag ttaggtagtt tgcaaacatg acttttgctg
     4921 gaggaacttt taatcatgtc ttattaattt ctggtcccaa taattaattt tcttacagtc
     4981 aaacccaatg cacttgaaaa ataatataat taatactgtg tgaagaattt tttgcatgtt
     5041 aaaaaaagca aaccactagt ttgcttagtt ctggcattca taaactactg taccttctgt
     5101 tctattgaca tgtttggaaa aaagaattga acatatgtac cacccccctc tttctagagt
     5161 ctccacagtt ctttagcaat tgctaaccat aggtttttaa tagcaaaaga caagattgtc
     5221 agtttttggt gcaaaactct actctttttg gttgacttgg aaaagctgtt gtttatattg
     5281 ggaattgtga cagctgtctt ttactttgtt aaattagcaa ttatttagca aaaatacaga
     5341 gtttcacccc agtaaaataa acttccacgt gagagttgtg ttcattcaag ggttattttt
     5401 tgagagtgga agtacatttc ctgcatttgt cacagataat atcagaaatt ttgttgtttt
     5461 gattaaaatg ttgccatcta cttataagtt aaaaatgctg agaaggtttt ttttggaaac
     5521 agtttatgag gccaggacat ttttaagttt gactcaaata taaaaagcca cagtattctt
     5581 gccatggttt tgtacttaga tgtctctcat tatggaattc agaatgtgac agttatagta
     5641 ctacatgaaa atacttaata tagagttcta cagttacagt attgcatgaa aatacagtat
     5701 ggtatgaaat tatttgtcca aaactgtact ccagaaatgg agcccatatg attatataac
     5761 tataaaatag gatgggacag agaaaaatat gtctgtatta cagcataaac atatctggtt
     5821 taatatgaac tatttctttt catctcttta ctctcagtat ctccataatg gccagtatgt
     5881 agtaggcacc tgtaattgtg aagcagcctc attgtctggg gtaaatacca aggttcttgg
     5941 tctcatggcc aaggagatcg aggtcgcaga cacacacagt ttggagcagg agtttaatag
     6001 gcaaaaggaa acagctctgt tacagagagg ggttccgagc aggttgccaa gttgtagtaa
     6061 aaatgtcagg gtttttataa atgggctagt gaggaggggg ctcatgagga ggggatatct
     6121 tatcctccta gggtccgagg acgctttagt tgggaccagg tgtgctatct gtacagagca
     6181 tagcttttta tcagctctta tcccattcct tgaccacata ggctcttaga ctcttattct
     6241 atgttgtctt cgtccagctt atctggaagg gagagtttct ctgtctgttc ccatacatct
     6301 tgcagctgca ggcatccccc cggtctgctt ttagcttcct tatcttagtg cccctaaagg
     6361 gaaaggaatg tgcttattaa gggccactgt tttactgggg ttaactgcat gaggtcaaaa
     6421 agagctatat ttgagctgct gtttgttaaa agaaaagttt tctgccgggg actcgcttta
     6481 ccctgtctac ctaaattctt tctgccttct ataacaattg tatgctcagt aaatacataa
     6541 ggaataatga acttaataac atttttttcc attctcaaaa tgatacttaa atgactcagt
     6601 acaacaaaga aactgagtag taaccagtgc aaaactagca aaaggatgaa atacacaaga
     6661 aaatgagaga atttaagagg agtagcaaaa cagcactgct aaagttacgg gtttatttta
     6721 aagtttaaaa tagaagtaaa caagcaaaca tactgataat ggttaggctt tctgtcccca
     6781 ccgcacaaat ctcatcttga attataatct caacatgtta agggagagac cagatgaaag
     6841 taattggatc atggggttgg tttctcccat gctgttctcg tgatagttcc catgagatct
     6901 gatggtttta taagtatttg gtagttcctc ctgcattcat tcttcctgct gccctgtgaa
     6961 gaggtgcctt ccaccatgat tgtgagtttc tggaggcttc tgcagccttg cggaactgtg
     7021 agtcaatcct ttttattata aattaccccg tctcaggcag ttctttatag cagtgtgggg
     7081 atggactaat actcgctggg ttagctgggg taggtaaagc cacagctgtg gcagcaacca
     7141 gctagtgggt gtcccttgat ggaggctttg caggctgcag cagaagggac ccttcagata
     7201 aacctccagt caggaaagga aaaggcccaa ataaacagta tttagatttt tttttaaagg
     7261 acataactac caatagagat ttaaaataat cataagaata ccattaactt tatgataaaa
     7321 attttgtaag tggagaagaa atggatatat ttctaggaaa acagtagaag atccatctaa
     7381 gattttgaca atttaaatca accactaagg aagtggaatc agtaatcaca agtccacctc
     7441 gccaaaagac aatttttgaa agatgaatgt taccaaaaca tcaagaaact gataattcct
     7501 gtcttataga aactttgaaa tgagagaaag ctacccaacc catttcagaa agttagtaaa
     7561 atctcaaagc tacacttgag caaagactgt gaaatattaa tagaccaatc ttgtttatga
     7621 acatagatgg aaaaattgct gatgaaatat tggcacattg aatgtagcag tgcataaaat
     7681 attgtatcat gagcaaaagg agaatctttc ccaggaacag gaggatgttt taataaaaat
     7741 atgtaattta cctctctaca gattaaagga gaaaaactat atgatcacat caacagaaac
     7801 aggaaattca gtttctttgg gggatagttg taattaaaag tgcttaactc acttatataa
     7861 gccattagtg gatgttacaa taaacctcgt atagtgtcag atgtaagaca gacatactgg
     7921 aattgtctat gaggttggat ccatgggcct gccatggaaa atttgttgag taatacgtcc
     7981 aaggcgtcac catttgccag gcgttgcaag tatcaccatt tgcaaggtgt taacaaaaag
     8041 ttacctgcct atggattctc ctttctaact ctgacctaat attttggagc atagaggaaa
     8101 gaaacatctt atgtttctgt gtttgggtaa acattagact tataaaaggg cctagggttt
     8161 ttttgtttgg taaaccattg cctttcctgc tttggtgtgg caaatcttcc tttggagaaa
     8221 aaggtccctt taatcttaaa accaactctc agtgaccaac aaaagcaaca aataggaaca
     8281 accacccatt aagaaccatc aggtgatgcc tcatttgctg tggtgcacag ctgaacttga
     8341 ttgttactct tggatcaggg ttggcttgat aaggactcac ttaaccaatg gaaagtaagg
     8401 ttaaatcttt gtaagcaatg ctcctgatgc gaatcacctg gagatcttga gcaaatgcag
     8461 actggttcag cagatctgga gtggggcctg agattctgca tttctaacaa gtttcctggt
     8521 gatattaatg ctgctggtct gtgagccata ctttgagtag caaaggacta aaggttgtga
     8581 gataaactgc ttttagcagc ccaaagggcc agctggaaag acgagaccag ttacagagca
     8641 aatggcaaga cagatctggt aatctgattc ttagagtact taaaaaagat aagaaggatc
     8701 tattcctgat atacagggta cttggtggat gtgtccatga cctcgagatc gaaaacccat
     8761 tagttaatct ttgtgacatc tgaattttaa ggatcttacc cagtgaacat ataactagtg
     8821 gaaatcctga taattggcta atgcaaggaa gtgataccca tgtgtaatgc ttatctttgt
     8881 atccatttca tggggaaggt catggaagac gacagcaaac ttgagtggtt aatgagagct
     8941 tcactaatgt tgtctgctgc aggcaataag tgaagtctct ggcagtggga agcatggacc
     9001 caagaccgaa agctctggca ctttatagca gtggtggggg gtagatagga cttagtgtgg
     9061 ccccttaaag agcaaggtcc aagtgatttt ctctggtgaa tgtcgatgtg gatctagtct
     9121 ccagggttag atccaggcag gtaacatcaa agtcagttga taagacctct tatgcctgtg
     9181 aatggaaggc cctgataaaa cttcattatt aatgttctgc attaatttag cggatgacta
     9241 tagtcatttc acataatcta atacatgaat gtcctgtatg ggtgactgct gagggtttaa
     9301 caaaggagag tccatttata tttggctggt aagatcgatg cttctgtcac tactgatggg
     9361 caaggatatt aacatcaata tctaaaaaaa ttctgagatt tggattgtga tgtgttaaat
     9421 ttctagacca atttgaggag aatttaacaa tagcttttca atttatgaac atggcgtata
     9481 tcttttttgg cggggggtga ggacagagtc ttgctctgag attacccagg ctggagttta
     9541 gtagcatgat cttggctcac tgcaacctct gcctcctgag ttgaagcaat tctcctgcct
     9601 cagcctcccc agtagctggg attacaggcg agcgccatca tgcctgacta attttcgtat
     9661 ttttagcaga gacagggtgt cgccacctca agggatccac ctgccttggc ctcccaaagt
     9721 gctgggattt ataagcgtga gtcaccgtac ctggccagac atagcatata tcttccttta
     9781 catagattgc ctttgatttc tttcattgtt actttgtagt tttcagctta cagattccgc
     9841 gcatattttg ttagacttaa atctaagtat ttgctttttt tttaacttac aaattttttt
     9901 aaaaaaacgt ttttcctctc caatgttcat tgctaataca tttcacgttt gaatctccag
     9961 ttcagaaaaa tcatggcctc tggagtttta ctgaggacta gagatagcta agtaacatag
    10021 ttccacctta ttcacttggt ctttgatgtg gtgtccataa ttgagtaaat ggcagtatgt
    10081 ccaatggcaa caccatgaac ttgactttgc cctcttgtct agccctctcc atttagactt
    10141 ggaagggtca gcagtaaaat ttcactggcc cagtccaggg ctgcttgagt gttactgttt
    10201 tgtgccacaa tatgatgtgg ccagactact tttctagttt cgagattatt ttggagcttt
    10261 atattgggta tgttcttatc caactatcat taggggcatt gatctctgat gcccttttct
    10321 ttcttatctt ccaaatggca ggcagtaaat ggcttattaa gcaagtataa gattcaagat
    10381 ctcagcccca aagatacatt tgtagatgcc aggcttaaca ttcctgaggc tctcagagaa
    10441 aacttttccc tctatagact ccccatttta agaaggaggc acagggcttg gttggcccat
    10501 ttagttagtg acacacctat attactctcc ttggcatctt accccagccc atccattggg
    10561 ttgtgtcagt cttttatttc taagtgagga tccagtcaac aggaggcaca agagatagaa
    10621 ccaaatacca taatgcctcc ttctattcca ctatgaacag tcaaggacac tggaggcctc
    10681 tgggtgggac acataggcgt tcaagagcct ctgagcttaa agatatgtga gtggagctaa
    10741 ctagactgtc caggtttggt ctacactgta attttttttt tttttttttt tttttggaag
    10801 cagagtcttg ctcttttgcc caggccagag tgtagtggtg tgatctcagc tcagtgcagc
    10861 gtctgcttcc tgggttcaag tgattctcct gcctcagcct ccaagtagct gggactacag
    10921 gtgcctgcca ccatgcccag ctaatttttg tatttttagt agagacgggg tttcaccgtg
    10981 ttggccaggc tggtctcgaa ctcctgagct caagtgatcc tcctgccttg gcctcccaaa
    11041 gtgttgggat agcaggcatg aaccaccgtg ctcagcctct ctgtaaatct tgagtcggca
    11101 ctgagctcat gaaagctgtg ctgagtgggt ttctttagat tttcattttc atgtttgccc
    11161 catttatgct aatattaatt attggaaaat aattcaccta ctttgcttct ctctgaccaa
    11221 cataggggca ggatctgtgg aaagacattt accacctgtt tgaaaaggtc agcgtgctga
    11281 tattcactaa ctactacccc cacaactccc cctcccccaa acctgataga tgctataatt
    11341 tctgcagtcc aatcactatt cttcccaaac tccatgagcc agattttata catactttgc
    11401 gtgaccataa aactctgtac tattatagac ataaaaccat ttaatttcat agataccttc
    11461 agtggtggca tcactgggtc ctctcttgga tagcatagtc ctatgaccaa atgggtcagt
    11521 cgttgatcag gcttcatgag aatatgatcg ctgggagaaa tatacagcta atttaacaac
    11581 aaatgactgt cctaattctg gagacctggg aagtattgat gagagtatag tgatcataag
    11641 cagacattgg gtgctttagc aaagccaatt tgcctgcatc ctctggaagg taaagaagat
    11701 ctcctctctc ttagaactta atcaaactct tggcatccac agaaggctag gaggatgtaa
    11761 tgaatactta ccatttttgt ggaagcccac aaccttttga atggcctttc tatactttag
    11821 tgaacctcca tgttatgaac cttacttcct caagtctctt ttgctgctag gaggcagaca
    11881 gtaacttcgg taattagaaa catctgcact cagcaacatg aagcacaagt tctgtgcagt
    11941 tgtccatatg ctgtcatgag ttttgccata tgtatcatct ggctttcaga ggacctttca
    12001 tggttgtcag gttaagttcc tgatacagaa gtggcatcag tgctggtgtt aatggctgca
    12061 gtaaagtcaa attcctagat tttagtgctc tgtggtagca gagagaatgg tggtagttta
    12121 ttttttcttt cctttttttt ttaaaaaaaa aaaaaaacct ttttcatgtt gaagttcaca
    12181 tgacctacaa ctaaccattt taaagtgtaa ttcagtgtca ttcagtacat ccacaatgtg
    12241 caatcaccac ctctgtctag ttccaaaaca tttttatcac cctaaaagga aaccttgcac
    12301 ccattaaaca gtggtagttt ctctatcatg ccacttttgt agcatgcttt ggggtcttat
    12361 tcctgggatc tttctctaca gtggtatgat tatataaggg tggaaatata aaagagtaag
    12421 cctttagaaa cagtttgtgt ctaaattagc tctcaggcat attgtcttca aaggaatgtt
    12481 ttaaaccagc caaagactaa catcaacatg cttacatttt gagtccaaaa ttaaaatcca
    12541 aaatagccaa gcatggtggc tcacgcctat aatcccaact actccagagg ctgaggtgtg
    12601 atagaatttc ttgaacctag gcgttccagg ctgcagtgag ctatgattgt gctgctgcac
    12661 tccagcctgg gtgacagagc gaggttgctt ctaaaacaaa aatttgaaat agtaagtgct
    12721 ttgatttttt ttaatataga atcaaacaat tgtgaaaata taattggcta agtttcatat
    12781 acagttcttt ctaaaactcc ttctgttctc taagatgtag tttgaaccag ccaaggatga
    12841 tggcttcaac atgtctccag ttgttttgcc actccagggc ctgactcaaa ccaaaataag
    12901 acttgctgtt accctgagac aagccaactg tcattgcttt agcaaccact acggctacag
    12961 gttgttgcat catgccaagc tctgtgttct ttctaactag tagaatggag tgggatggta
    13021 tcgtgatggt gaacccaaaa caatggtact aggaaggagg ttggccaaac tcagagtgga
    13081 ggctgaccct gtatctggtt gtagcagagt cggtcctagg ggaacaggct cagcagcagc
    13141 aaggatgcaa ggaccagaag gcagcagggg aaggaaaagg agcaggggat aggggaactt
    13201 taggagcaga gtagtgttga taactggcat ctggaaccta cagtttccag ttcagagcag
    13261 tacaccatac tcagtgtata cttacactcc aagacatttc ctgggcagtg atctagaaat
    13321 gatattttta aaaggagctg tgtttcagtg ttagactgta gttatccaag cagtatcaga
    13381 tagtgtgcag tttttgtagt aaaaggtaaa aatctgaata ataaagttta ttaataacag
    13441 ttttagttaa tacttgactg acattgagac ccttgatgca aaaaggtaag tagaagagaa
    13501 ggctgtaaac acaagaccat cataccctgt ggggaattta caatctaaat ataacaaaca
    13561 tatagagtga gataggagaa aagaaatggg atgataatga tattgctgta ctaaagtcta
    13621 tttaggaaat ggaaatatat agcaagagga gtcagctact tgggcattga tcaaagaaaa
    13681 ataaacatat agaaggaaag aaaggtcgtt ttagtttata acattttcct tccgattcta
    13741 ggtgattata tagctagttt actcttccac ccccaagatt ctggggtatt ttggctaatg
    13801 ttatttgcaa tgtccataaa ctcagaagaa gttagtatta acggggatat gaaagaacct
    13861 ccagttacta tgcaagaaat tttaaatttc catcatgtta aattgtacaa ctgtttatca
    13921 ttcactctgc tgtaagtcta tgtgaaacca aatccgtgga tttaccaatg gccattttat
    13981 cacctccaaa ctatctttta tttttttgaa ttgtatctct ctccttattc cattttcttc
    14041 tgtttgatta aaatcctgca tgttctcctg ca
//
