LOCUS       NM_005313               3680 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens protein disulfide isomerase family A member 3 (PDIA3),
            mRNA.
ACCESSION   NM_005313
VERSION     NM_005313.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Lin,J., Lyu,Z., Feng,H., Xie,H., Peng,J., Zhang,W., Zheng,J.,
            Zheng,J., Pan,Z. and Li,Y.
  TITLE     CircPDIA3/miR-449a/XBP1 feedback loop curbs pyroptosis by
            inhibiting palmitoylation of the GSDME-C domain to induce
            chemoresistance of colorectal cancer
  JOURNAL   Drug Resist Updat 76, 101097 (2024)
   PUBMED   38861804
  REMARK    GeneRIF: CircPDIA3/miR-449a/XBP1 feedback loop curbs pyroptosis by
            inhibiting palmitoylation of the GSDME-C domain to induce
            chemoresistance of colorectal cancer.
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wu,W., Peng,G., Wang,K., Yang,Y., Liu,Z. and Xiao,G.
  TITLE     Single-cell analysis identified PDIA3 as regulator of malignant
            characteristics and macrophage function in human cancers
  JOURNAL   Funct Integr Genomics 24 (4), 136 (2024)
   PUBMED   39138692
  REMARK    GeneRIF: Single-cell analysis identified PDIA3 as regulator of
            malignant characteristics and macrophage function in human cancers.
            Erratum:[Funct Integr Genomics. 2024 Sep 2;24(5):151. doi:
            10.1007/s10142-024-01439-3.. PMID: 39222202]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3680)
  AUTHORS   Fan,J., Wang,L., Zhang,C., Wu,X., Han,L., Zhang,X., Gao,S., Xue,J.
            and Zhang,Q.
  TITLE     PDIA3 driven STAT3/PD-1 signaling promotes M2 TAM polarization and
            aggravates colorectal cancer progression
  JOURNAL   Aging (Albany NY) 16 (10), 8880-8897 (2024)
   PUBMED   38761176
  REMARK    GeneRIF: PDIA3 driven STAT3/PD-1 signaling promotes M2 TAM
            polarization and aggravates colorectal cancer progression.
REFERENCE   4  (bases 1 to 3680)
  AUTHORS   Yang,M., Li,Q., Yang,H., Li,Y., Lu,L., Wu,X., Liu,Y., Li,W.,
            Shen,J., Xiao,Z., Zhao,Y., Du,F., Chen,Y., Deng,S., Cho,C.H., Li,X.
            and Li,M.
  TITLE     Downregulation of PDIA3 inhibits gastric cancer cell growth through
            cell cycle regulation
  JOURNAL   Biomed Pharmacother 173, 116336 (2024)
   PUBMED   38412717
  REMARK    GeneRIF: Downregulation of PDIA3 inhibits gastric cancer cell
            growth through cell cycle regulation.
REFERENCE   5  (bases 1 to 3680)
  AUTHORS   Nowak,J.I., Olszewska,A.M., Piotrowska,A., Myszczynski,K.,
            Domzalski,P. and Zmijewski,M.A.
  TITLE     PDIA3 modulates genomic response to 1,25-dihydroxyvitamin D3 in
            squamous cell carcinoma of the skin
  JOURNAL   Steroids 199, 109288 (2023)
   PUBMED   37549780
  REMARK    GeneRIF: PDIA3 modulates genomic response to 1,25-dihydroxyvitamin
            D3 in squamous cell carcinoma of the skin.
REFERENCE   6  (bases 1 to 3680)
  AUTHORS   Hirano,N., Shibasaki,F., Sakai,R., Tanaka,T., Nishida,J.,
            Yazaki,Y., Takenawa,T. and Hirai,H.
  TITLE     Molecular cloning of the human glucose-regulated protein
            ERp57/GRP58, a thiol-dependent reductase. Identification of its
            secretory form and inducible expression by the oncogenic
            transformation
  JOURNAL   Eur J Biochem 234 (1), 336-342 (1995)
   PUBMED   8529662
REFERENCE   7  (bases 1 to 3680)
  AUTHORS   Bourdi,M., Demady,D., Martin,J.L., Jabbour,S.K., Martin,B.M.,
            George,J.W. and Pohl,L.R.
  TITLE     cDNA cloning and baculovirus expression of the human liver
            endoplasmic reticulum P58: characterization as a protein disulfide
            isomerase isoform, but not as a protease or a carnitine
            acyltransferase
  JOURNAL   Arch Biochem Biophys 323 (2), 397-403 (1995)
   PUBMED   7487104
REFERENCE   8  (bases 1 to 3680)
  AUTHORS   Hochstrasser,D.F., Frutiger,S., Paquet,N., Bairoch,A., Ravier,F.,
            Pasquali,C., Sanchez,J.C., Tissot,J.D., Bjellqvist,B., Vargas,R. et
            al.
  TITLE     Human liver protein map: a reference database established by
            microsequencing and gel comparison
  JOURNAL   Electrophoresis 13 (12), 992-1001 (1992)
   PUBMED   1286669
REFERENCE   9  (bases 1 to 3680)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   10 (bases 1 to 3680)
  AUTHORS   Dawson,S.J. and White,L.A.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J Infect 24 (3), 317-320 (1992)
   PUBMED   1602151
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG826781.1, BC014433.1,
            BM854408.1, BE644608.1, AI769725.1 and AC018512.6.
            
            On Nov 23, 2018 this sequence version replaced NM_005313.4.
            
            Summary: This gene encodes a protein of the endoplasmic reticulum
            that interacts with lectin chaperones calreticulin and calnexin to
            modulate folding of newly synthesized glycoproteins. The protein
            was once thought to be a phospholipase; however, it has been
            demonstrated that the protein actually has protein disulfide
            isomerase activity. It is thought that complexes of lectins and
            this protein mediate protein folding by promoting formation of
            disulfide bonds in their glycoprotein substrates. This protein also
            functions as a molecular chaperone that prevents the formation of
            protein aggregates. [provided by RefSeq, Dec 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.215983.1,
                                           SRR1660803.78112.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300289.10/ ENSP00000300289.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                BG826781.1         48-60
            14-1947             BC014433.1         1-1934
            1948-2346           BM854408.1         56-454
            2347-2865           BE644608.1         4-522               c
            2866-3008           AI769725.1         7-149               c
            3009-3680           AC018512.6         87805-88476
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.3"
     gene            1..3680
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /note="protein disulfide isomerase family A member 3"
                     /db_xref="GeneID:2923"
                     /db_xref="HGNC:HGNC:4606"
                     /db_xref="MIM:602046"
     exon            1..269
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1620
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /EC_number="5.3.4.1"
                     /note="protein disulfide isomerase-associated 3; glucose
                     regulated protein, 58kDa; protein disulfide-isomerase A3;
                     58 kDa microsomal protein; endoplasmic reticulum P58;
                     phospholipase C-alpha; disulfide isomerase ER-60; 58 kDa
                     glucose-regulated protein; ER protein 57; ER protein 60;
                     endoplasmic reticulum resident protein 57; endoplasmic
                     reticulum resident protein 60; epididymis secretory
                     protein Li 269; epididymis secretory sperm binding protein
                     Li 93n"
                     /codon_start=1
                     /product="protein disulfide-isomerase A3 precursor"
                     /protein_id="NP_005304.3"
                     /db_xref="CCDS:CCDS10101.1"
                     /db_xref="GeneID:2923"
                     /db_xref="HGNC:HGNC:4606"
                     /db_xref="MIM:602046"
                     /translation="MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGS
                     AGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPT
                     LKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFF
                     DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDK
                     TVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGS
                     NYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGE
                     KFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNN
                     ENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGF
                     PTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL"
     exon            270..348
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            349..466
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            467..574
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            575..704
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            705..821
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            822..947
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1130
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1239
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1368
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1448
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1506
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..3680
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1922..1927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /note="hexamer: AATAAA"
     polyA_site      1942
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /note="major polyA site"
     regulatory      2843..2848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /note="hexamer: AGTAAA"
     polyA_site      2865
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
     regulatory      3657..3662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
                     /note="hexamer: AGTAAA"
     polyA_site      3680
                     /gene="PDIA3"
                     /gene_synonym="ER60; ERp57; ERp60; ERp61; GRP57; GRP58;
                     HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC"
ORIGIN      
        1 agacgcgcga gcgcaagcag cgggttagtg gtcgcgcgcc cgacctccgc agtcccagcc
       61 gagccgcgac ccttccggcc gtccccaccc cacctcgccg ccatgcgcct ccgccgccta
      121 gcgctgttcc cgggtgtggc gctgcttctt gccgcggccc gcctcgccgc tgcctccgac
      181 gtgctagaac tcacggacga caacttcgag agtcgcatct ccgacacggg ctctgcgggc
      241 ctcatgctcg tcgagttctt cgccccctgg tgtggacact gcaagagact tgcacctgag
      301 tatgaagctg cagctaccag attaaaagga atagtcccat tagcaaaggt tgattgcact
      361 gccaacacta acacctgtaa taaatatgga gtcagtggat atccaaccct gaagatattt
      421 agagatggtg aagaagcagg tgcttatgat ggacctagga ctgctgatgg aattgtcagc
      481 cacttgaaga agcaggcagg accagcttca gtgcctctca ggactgagga agaatttaag
      541 aaattcatta gtgataaaga tgcctctata gtaggttttt tcgatgattc attcagtgag
      601 gctcactccg agttcctaaa agcagccagc aacttgaggg ataactaccg atttgcacat
      661 acgaatgttg agtctctggt gaacgagtat gatgataatg gagagggtat catcttattt
      721 cgtccttcac atctcactaa caagtttgag gacaagactg tggcatatac agagcaaaaa
      781 atgaccagtg gcaaaattaa aaagtttatc caggaaaaca tttttggtat ctgccctcac
      841 atgacagaag acaataaaga tttgatacag ggcaaggact tacttattgc ttactatgat
      901 gtggactatg aaaagaacgc taaaggttcc aactactgga gaaacagggt aatgatggtg
      961 gcaaagaaat tcctggatgc tgggcacaaa ctcaactttg ctgtagctag ccgcaaaacc
     1021 tttagccatg aactttctga ttttggcttg gagagcactg ctggagagat tcctgttgtt
     1081 gctatcagaa ctgctaaagg agagaagttt gtcatgcagg aggagttctc gcgtgatggg
     1141 aaggctctgg agaggttcct gcaggattac tttgatggca atctgaagag atacctgaag
     1201 tctgaaccta tcccagagag caatgatggg cctgtgaagg tagtggtagc agagaatttt
     1261 gatgaaatag tgaataatga aaataaagat gtgctgattg aattttatgc cccttggtgt
     1321 ggtcactgta agaacctgga gcccaagtat aaagaacttg gcgagaagct cagcaaagac
     1381 ccaaatatcg tcatagccaa gatggatgcc acagccaatg atgtgccttc tccatatgaa
     1441 gtcagaggtt ttcctaccat atacttctct ccagccaaca agaagctaaa tccaaagaaa
     1501 tatgaaggtg gccgtgaatt aagtgatttt attagctatc tacaaagaga agctacaaac
     1561 ccccctgtaa ttcaagaaga aaaacccaag aagaagaaga aggcacagga ggatctctaa
     1621 agcagtagcc aaacaccact ttgtaaaagg actcttccat cagagatggg aaaaccattg
     1681 gggaggacta ggacccatat gggaattatt acctctcagg gccgagagga cagaatggat
     1741 ataatctgaa tcctgttaaa ttttctctaa actgtttctt agctgcactg tttatggaaa
     1801 taccaggacc agtttatgtt tgtggttttg ggaaaaatta tttgtgttgg gggaaatgtt
     1861 gtgggggtgg ggttgagttg ggggtatttt ctaatttttt ttgtacattt ggaacagtga
     1921 caataaatga gaccccttta aactgtctta ttttccacca gattgagaac cagatgttct
     1981 ctacacacta tcactgttca atagagcttt cttcagtgat ggaaatgctc tgtaatctac
     2041 actgttcagt acaggtagct acggagcatc tgaaatatgg ctagaaacta catttttgtt
     2101 ttgattaatt taaatagcca tacagttgct accatactgg tcacggcagc tgtagactga
     2161 ctgggtccat agttcatcac ctcaaaattc tttcaaaatt tattatctct ttctctcctt
     2221 acatgtttat ttcccaggcc taccctggtg attagaacag ctgaagggcc tttcttgtta
     2281 ggctgtccat gccctaagga tgggttcctg tttatccttg ccacgcagct gagcttactg
     2341 catgtttata tctcccaagg actgttctct gctcagaaat gccctgtcaa gggtgtggca
     2401 tcacgcagtt tcatccaagt tgtttcagga attgctgaca ctgctgggtg cagttctatc
     2461 ccctaaagcc tagggtgtgg ccctttaact tccccttcag tagaactggg aaaggcagta
     2521 ccattcctag ttatgagtga agcatccact ttcttttgta acaatgagga acagaaagga
     2581 taagactgaa gagtgatctt ttgtccaact aaaccattta tctccttttg taggtaaatt
     2641 caaatcctgc ccagttatag ttttcagtca ctggagaatt ccaggtagga gccctacttt
     2701 aggtgatcct aggaactcct atgttcagaa aagaattttc ttcctactat ataattacag
     2761 tatttagctg tcaattttaa gatgaatttg gtagagcctt atagtaaagt atgtatcttg
     2821 gtcacacaca aagcttggaa aaagtaaaag atgtctaaac cataatcttg taactcataa
     2881 catctgggct ggggcccggg gatctaattg tttagagagc cccaaaagtg gctcagatgt
     2941 aaagccaggg ttaagaacca tctgccttgg aagatttaag ggaaacatat aaacttgtac
     3001 aaaggacaca gaagcagtca gttttaattt tttagccatg ttggtaaaag ttcattttca
     3061 gtacatgggt aacacccagg ccctttccca ttatatccag gtatgctaca agttctttta
     3121 actcttatca gaagttatta ttactgtttc cttagagagg ctaccaggct aaaattcact
     3181 tagtttggtt tgtctaatgt cctcattatt ttatcctgaa gatgatgtca tttctcagga
     3241 cttgaaaatg acttggctga actaaaggta aaaaagccaa gcctctgtca cttttcctag
     3301 actcctaggc acagctatgg agtctttgca cagtgcccat accctaaaaa ttaataatga
     3361 aaaccaaacc tcaagaacct agagcagctc tctacttgcc accatggact ccagtggtca
     3421 gcataagaaa agcagatagt tgcattctat ttagtttata gctgctttgt tcctttgtgt
     3481 ttcactaagc agaggctcaa aaattccctt gataacttca gctgcccctg ttcttttcct
     3541 caaactccag gatgagacct ttaatgtggg acaatttctg gtgaaggtac tcacagcgac
     3601 gcttttcttc tctgtaactt gggtactgct gcagagaaaa agcatccatg tcaaaaagta
     3661 aaaattctca ttctaccttg
//
