LOCUS       NM_033023               2742 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens platelet derived growth factor subunit A (PDGFA),
            transcript variant 2, mRNA.
ACCESSION   NM_033023 XM_926001 XM_930866 XM_935642 XM_942520 XM_946069
VERSION     NM_033023.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wang,K., Li,C., Chen,H., Gu,P., Lu,J., Zhao,H. and Zhuo,X.
  TITLE     Increased Expression of PDGFA Is Associated With Poor Prognosis and
            Immune Infiltration in Head and Neck Squamous Cell Carcinoma
  JOURNAL   J Oral Pathol Med 53 (9), 584-594 (2024)
   PUBMED   39295197
  REMARK    GeneRIF: Increased Expression of PDGFA Is Associated With Poor
            Prognosis and Immune Infiltration in Head and Neck Squamous Cell
            Carcinoma.
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Hammerschmidt,T.G., Encarnacao,M., Lamberty Faverzani,J., de Fatima
            Lopes,F., Poswar de Oliveira,F., Fischinger Moura de Sousa,C.,
            Ribeiro,I., Alves,S., Giugliani,R. and Regla Vargas,C.
  TITLE     Molecular profile and peripheral markers of neurodegeneration in
            patients with Niemann-Pick type C: Decrease in Plasminogen
            Activator Inhibitor type 1 and Platelet-Derived Growth Factor type
            AA
  JOURNAL   Arch Biochem Biophys 735, 109510 (2023)
   PUBMED   36608914
  REMARK    GeneRIF: Molecular profile and peripheral markers of
            neurodegeneration in patients with Niemann-Pick type C: Decrease in
            Plasminogen Activator Inhibitor type 1 and Platelet-Derived Growth
            Factor type AA.
REFERENCE   3  (bases 1 to 2742)
  AUTHORS   Tsai,Y.C., Kuo,T.N., Chao,Y.Y., Lee,P.R., Lin,R.C., Xiao,X.Y.,
            Huang,B.M. and Wang,C.Y.
  TITLE     PDGF-AA activates AKT and ERK signaling for testicular interstitial
            Leydig cell growth via primary cilia
  JOURNAL   J Cell Biochem 124 (1), 89-102 (2023)
   PUBMED   36306470
  REMARK    GeneRIF: PDGF-AA activates AKT and ERK signaling for testicular
            interstitial Leydig cell growth via primary cilia.
REFERENCE   4  (bases 1 to 2742)
  AUTHORS   Cui,H.Y., Wei,W., Qian,M.R., Tian,R.F., Fu,X., Li,H.W., Nan,G.,
            Yang,T., Lin,P., Chen,X., Zhu,Y.M., Wang,B., Sun,X.X., Dou,J.H.,
            Jiang,J.L., Li,L., Wang,S.J. and Chen,Z.N.
  TITLE     PDGFA-associated protein 1 is a novel target of c-Myc and
            contributes to colorectal cancer initiation and progression
  JOURNAL   Cancer Commun (Lond) 42 (8), 750-767 (2022)
   PUBMED   35716012
  REMARK    GeneRIF: PDGFA-associated protein 1 is a novel target of c-Myc and
            contributes to colorectal cancer initiation and progression.
REFERENCE   5  (bases 1 to 2742)
  AUTHORS   Demoulin,J.B. and Essaghir,A.
  TITLE     PDGF receptor signaling networks in normal and cancer cells
  JOURNAL   Cytokine Growth Factor Rev 25 (3), 273-283 (2014)
   PUBMED   24703957
  REMARK    Review article
REFERENCE   6  (bases 1 to 2742)
  AUTHORS   Ishibashi,T., Bottaro,D.P., Chan,A., Miki,T. and Aaronson,S.A.
  TITLE     Expression cloning of a human dual-specificity phosphatase
  JOURNAL   Proc Natl Acad Sci U S A 89 (24), 12170-12174 (1992)
   PUBMED   1281549
REFERENCE   7  (bases 1 to 2742)
  AUTHORS   Betsholtz,C., Johnsson,A., Heldin,C.H., Westermark,B., Lind,P.,
            Urdea,M.S., Eddy,R., Shows,T.B., Philpott,K., Mellor,A.L. et al.
  TITLE     cDNA sequence and chromosomal localization of human
            platelet-derived growth factor A-chain and its expression in tumour
            cell lines
  JOURNAL   Nature 320 (6064), 695-699 (1986)
   PUBMED   3754619
REFERENCE   8  (bases 1 to 2742)
  AUTHORS   Heldin,C.H., Westermark,B. and Wasteson,A.
  TITLE     Platelet-derived growth factor: purification and partial
            characterization
  JOURNAL   Proc Natl Acad Sci U S A 76 (8), 3722-3726 (1979)
   PUBMED   291037
REFERENCE   9  (bases 1 to 2742)
  AUTHORS   Antoniades,H.N., Scher,C.D. and Stiles,C.D.
  TITLE     Purification of human platelet-derived growth factor
  JOURNAL   Proc Natl Acad Sci U S A 76 (4), 1809-1813 (1979)
   PUBMED   287022
REFERENCE   10 (bases 1 to 2742)
  AUTHORS   Nath,K., Gogi,R., Khan,A.A. and Hameed,S.
  TITLE     Vascular hamartoma and vascular tumours of orbit
  JOURNAL   Indian J Ophthalmol 25 (1), 18-23 (1977)
   PUBMED   612586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147651.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 17, 2020 this sequence version replaced NM_033023.4.
            
            Summary: This gene encodes a member of the protein family comprised
            of both platelet-derived growth factors (PDGF) and vascular
            endothelial growth factors (VEGF). The encoded preproprotein is
            proteolytically processed to generate platelet-derived growth
            factor subunit A, which can homodimerize, or alternatively,
            heterodimerize with the related platelet-derived growth factor
            subunit B. These proteins bind and activate PDGF receptor tyrosine
            kinases, which play a role in a wide range of developmental
            processes. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (2) lacks exon 6, compared to
            variant 1, and encodes the shorter isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and experimental evidence.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X06374.1, SRR14038192.1986271.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402802.8/ ENSP00000383889.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-908               AC147651.4         47535-48442         c
            909-1005            AC147651.4         45990-46086         c
            1006-1110           AC147651.4         40947-41051         c
            1111-1298           AC147651.4         39405-39592         c
            1299-1425           AC147651.4         29712-29838         c
            1426-2742           AC147651.4         25854-27170         c
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.3"
     gene            1..2742
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /note="platelet derived growth factor subunit A"
                     /db_xref="GeneID:5154"
                     /db_xref="HGNC:HGNC:8799"
                     /db_xref="MIM:173430"
     exon            1..908
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             846..1436
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; PDGF A-chain; platelet-derived growth factor
                     alpha chain; PDGF subunit A; platelet-derived growth
                     factor alpha polypeptide; platelet-derived growth factor
                     A-chain"
                     /codon_start=1
                     /product="platelet-derived growth factor subunit A isoform
                     2 preproprotein"
                     /protein_id="NP_148983.1"
                     /db_xref="CCDS:CCDS47524.1"
                     /db_xref="GeneID:5154"
                     /db_xref="HGNC:HGNC:8799"
                     /db_xref="MIM:173430"
                     /translation="MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDL
                     QRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVI
                     YEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRK
                     KPKLKEVQVRLEEHLECACATTSLNPDYREEDTDVR"
     exon            909..1005
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1110
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1298
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1425
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..2742
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2240..2245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /note="hexamer: AATAAA"
     polyA_site      2269
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /note="major polyA site"
     regulatory      2715..2720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
                     /note="hexamer: ATTAAA"
     polyA_site      2742
                     /gene="PDGFA"
                     /gene_synonym="PDGF-A; PDGF1"
ORIGIN      
        1 acacgcgcgc cctgcggagc ccgcccaact ccggcgagcc gggcctgcgc ctactcctcc
       61 tcctcctctc ccggcggcgg ctgcggcgga ggcgccgact cggccttgcg cccgccctca
      121 ggcccgcgcg ggcggcgcag cgaggccccg ggcggcgggt ggtggctgcc aggcggctcg
      181 gccgcgggcg ctgcccggcc ccggcgagcg gagggcggag cgcggcgccg gagccgaggg
      241 cgcgccgcgg agggggtgct gggccgcgct gtgcccggcc gggcggcggc tgcaagagga
      301 ggccggaggc gagcgcgggg ccggcggtgg gcgcgcaggg cggctcgcag ctcgcagccg
      361 gggccgggcc aggcgtccag gcaggtgatc ggtgtggcgg cggcggcggc ggcggcccca
      421 gactccctcc ggagttcttc ttggggctga tgtccgcaaa tatgcagaat taccggccgg
      481 gtcgctcctg aagccagcgc ggggagcgag cgcggcggcg gccagcaccg ggaacgcacc
      541 gaggaagaag cccagccccc gccctccgcc ccttccgtcc ccacccccta cccggcggcc
      601 caggaggctc cccgcgctgc gggcgcgcac tccctgtttc tcctcctcct ggctggcgct
      661 gcctgcctct ccgcactcac tgctcgcgcc gggcgcgctc cgccagctcc gtgctccccg
      721 cgccaccctc ctccgggccg cgctccctaa gggatggtac tgaatttcgc cgccacagga
      781 gaccggctgg agcgcccgcc ccgcggcctc gcctctcctc cgagcagcca gcgcctcggg
      841 acgcgatgag gaccttggct tgcctgctgc tcctcggctg cggatacctc gcccatgttc
      901 tggccgagga agccgagatc ccccgcgagg tgatcgagag gctggcccgc agtcagatcc
      961 acagcatccg ggacctccag cgactcctgg agatagactc cgtagggagt gaggattctt
     1021 tggacaccag cctgagagct cacggggtcc atgccactaa gcatgtgccc gagaagcggc
     1081 ccctgcccat tcggaggaag agaagcatcg aggaagctgt ccccgctgtc tgcaagacca
     1141 ggacggtcat ttacgagatt cctcggagtc aggtcgaccc cacgtccgcc aacttcctga
     1201 tctggccccc gtgcgtggag gtgaaacgct gcaccggctg ctgcaacacg agcagtgtca
     1261 agtgccagcc ctcccgcgtc caccaccgca gcgtcaaggt ggccaaggtg gaatacgtca
     1321 ggaagaagcc aaaattaaaa gaagtccagg tgaggttaga ggagcatttg gagtgcgcct
     1381 gcgcgaccac aagcctgaat ccggattatc gggaagagga cacggatgtg aggtgaggat
     1441 gagccgcagc cctttcctgg gacatggatg tacatggcgt gttacattcc tgaacctact
     1501 atgtacggtg ctttattgcc agtgtgcggt ctttgttctc ctccgtgaaa aactgtgtcc
     1561 gagaacactc gggagaacaa agagacagtg cacatttgtt taatgtgaca tcaaagcaag
     1621 tattgtagca ctcggtgaag cagtaagaag cttccttgtc aaaaagagag agagagaaag
     1681 agagagagaa aacaaaacca caaatgacaa aaacaaaacg gactcacaaa aatatctaaa
     1741 ctcgatgaga tggagggtcg ccccgtggga tggaagtgca gaggtctcag cagactggat
     1801 ttctgtccgg gtggtcacag gtgctttttt gccgaggatg cagagcctgc tttgggaacg
     1861 actccagagg ggtgctggtg ggctctgcag gggcccgcag gaagcaggaa tgtcttggaa
     1921 accgccacgc gaactttaga aaccacacct cctcgctgta gtatttaagc ccatacagaa
     1981 accttcctga gagccttaag tggttttttt ttttgttttt gttttgtttt tttttttttt
     2041 gttttttttt tttttttttt acaccataaa gtgattatta agctttcctt tttactcttt
     2101 ggctagcttt tttttttttt tttttttttt aattatctct tggatgacat ttacaccgat
     2161 aacacacagg ctgctgtaac tgtcaggaca gtgcgacggt atttttccta gcaagatgca
     2221 aactaatgag atgtattaaa ataaacatgg tatacctacc tatgcatcat ttcctaaatg
     2281 tttctggctt tgtgtttctc ccttaccctg ctttatttgt taatttaagc cattttgaaa
     2341 gaactatgcg tcaaccaatc gtacgccgtc cctgcggcac ctgccccaga gcccgtttgt
     2401 ggctgagtga caacttgttc cccgcagtgc acacctagaa tgctgtgttc ccacgcggca
     2461 cgtgagatgc attgccgctt ctgtctgtgt tgttggtgtg ccctggtgcc gtggtggcgg
     2521 tcactccctc tgctgccagt gtttggacag aacccaaatt ctttattttt ggtaagatat
     2581 tgtgctttac ctgtattaac agaaatgtgt gtgtgtggtt tgtttttttg taaaggtgaa
     2641 gtttgtatgt ttacctaata ttacctgttt tgtatacctg agagcctgct atgttctttt
     2701 tttgttgatc caaaattaaa aaaaaaaata ccaccaacaa aa
//
