LOCUS       NM_003719               4606 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens phosphodiesterase 8B (PDE8B), transcript variant 1,
            mRNA.
ACCESSION   NM_003719
VERSION     NM_003719.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   He,Z., Peng,Y., Wang,D., Yang,C., Zhou,C., Gong,B., Song,S. and
            Wang,Y.
  TITLE     Single-cell transcriptomic analysis identifies downregulated
            phosphodiesterase 8B as a novel oncogene in IDH-mutant glioma
  JOURNAL   Front Immunol 15, 1427200 (2024)
   PUBMED   38989284
  REMARK    GeneRIF: Single-cell transcriptomic analysis identifies
            downregulated phosphodiesterase 8B as a novel oncogene in
            IDH-mutant glioma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Campolo,F., Capponi,C., Tarsitano,M.G., Tenuta,M., Pozza,C.,
            Gianfrilli,D., Magliocca,F., Venneri,M.A., Vicini,E., Lenzi,A.,
            Isidori,A.M. and Barbagallo,F.
  TITLE     cAMP-specific phosphodiesterase 8A and 8B isoforms are
            differentially expressed in human testis and Leydig cell tumor
  JOURNAL   Front Endocrinol (Lausanne) 13, 1010924 (2022)
   PUBMED   36277728
  REMARK    GeneRIF: cAMP-specific phosphodiesterase 8A and 8B isoforms are
            differentially expressed in human testis and Leydig cell tumor.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4606)
  AUTHORS   Ni,J., Yi,X., Liu,Z., Sun,W., Yuan,Y., Yang,J., Jiang,H., Shen,L.,
            Tang,B., Liu,Y. and Wang,J.
  TITLE     Clinical findings of autosomal-dominant striatal degeneration and
            PDE8B mutation screening in parkinsonism and related disorders
  JOURNAL   Parkinsonism Relat Disord 69, 94-98 (2019)
   PUBMED   31726290
  REMARK    GeneRIF: Clinical findings of autosomal-dominant striatal
            degeneration and PDE8B mutation screening in parkinsonism and
            related disorders.
REFERENCE   4  (bases 1 to 4606)
  AUTHORS   Fan,T.S., Wu,R.M., Lin,H.I., Cheng,C. and Lin,C.H.
  TITLE     PDE8B mutation is not associated with Parkinson's disease in a
            Taiwanese population
  JOURNAL   Neurobiol Aging 71, 265 (2018)
   PUBMED   29909144
  REMARK    GeneRIF: Study shows that PDE8B may not play a major role in
            familial and early-onset Parkinson disease without distinctive
            lesions of the striatum on brain MRI in this Taiwanese population.
REFERENCE   5  (bases 1 to 4606)
  AUTHORS   Soto-Pedre,E., Siddiqui,M.K., Doney,A.S., Palmer,C.N.A.,
            Pearson,E.R. and Leese,G.P.
  TITLE     Replication confirms the association of loci in FOXE1, PDE8B, CAPZB
            and PDE10A with thyroid traits: a Genetics of Diabetes Audit and
            Research Tayside study (GoDARTS)
  JOURNAL   Pharmacogenet Genomics 27 (10), 356-362 (2017)
   PUBMED   28727628
  REMARK    GeneRIF: replication confirmed at genome-wide significance the
            association of loci at FOXE1 with hypothyroidism, and PDE8B, CAPZB
            and PDE10A with serum TSH. A total of 12 SNPs seemed to explain
            nearly 7% of the serum TSH variation
REFERENCE   6  (bases 1 to 4606)
  AUTHORS   Perez-Torres,S., Cortes,R., Tolnay,M., Probst,A., Palacios,J.M. and
            Mengod,G.
  TITLE     Alterations on phosphodiesterase type 7 and 8 isozyme mRNA
            expression in Alzheimer's disease brains examined by in situ
            hybridization
  JOURNAL   Exp Neurol 182 (2), 322-334 (2003)
   PUBMED   12895443
  REMARK    GeneRIF: In Alzheimer's disease brains we found that PDE8B was the
            only PDE isozyme showing a significant increase, in cortical areas
            and parts of the hippocampal formation, at Braak stages III-VI
REFERENCE   7  (bases 1 to 4606)
  AUTHORS   Gamanuma,M., Yuasa,K., Sasaki,T., Sakurai,N., Kotera,J. and
            Omori,K.
  TITLE     Comparison of enzymatic characterization and gene organization of
            cyclic nucleotide phosphodiesterase 8 family in humans
  JOURNAL   Cell Signal 15 (6), 565-574 (2003)
   PUBMED   12681444
  REMARK    GeneRIF: Comparison of enzymatic characterization and gene
            organization of PDE8B and PDE8A.
REFERENCE   8  (bases 1 to 4606)
  AUTHORS   Hayashi,M., Shimada,Y., Nishimura,Y., Hama,T. and Tanaka,T.
  TITLE     Genomic organization, chromosomal localization, and alternative
            splicing of the human phosphodiesterase 8B gene
  JOURNAL   Biochem Biophys Res Commun 297 (5), 1253-1258 (2002)
   PUBMED   12372422
  REMARK    GeneRIF: selective usage of exons produces three different PDE8B
            variants that exhibit a tissue-specific expression pattern
REFERENCE   9  (bases 1 to 4606)
  AUTHORS   Persani,L., Borgato,S., Lania,A., Filopanti,M., Mantovani,G.,
            Conti,M. and Spada,A.
  TITLE     Relevant cAMP-specific phosphodiesterase isoforms in human
            pituitary: effect of Gs(alpha) mutations
  JOURNAL   J Clin Endocrinol Metab 86 (8), 3795-3800 (2001)
   PUBMED   11502813
REFERENCE   10 (bases 1 to 4606)
  AUTHORS   Hayashi,M., Matsushima,K., Ohashi,H., Tsunoda,H., Murase,S.,
            Kawarada,Y. and Tanaka,T.
  TITLE     Molecular cloning and characterization of human PDE8B, a novel
            thyroid-specific isozyme of 3',5'-cyclic nucleotide
            phosphodiesterase
  JOURNAL   Biochem Biophys Res Commun 250 (3), 751-756 (1998)
   PUBMED   9784418
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022414.8, AC022422.5,
            KF457931.1, KC877015.1 and AC010234.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 8, 2019 this sequence version replaced NM_003719.4.
            
            Summary: The protein encoded by this gene is a cyclic nucleotide
            phosphodiesterase (PDE) that catalyzes the hydrolysis of the second
            messenger cAMP. The encoded protein, which does not hydrolyze cGMP,
            is resistant to several PDE inhibitors. Defects in this gene are a
            cause of autosomal dominant striatal degeneration (ADSD). Several
            transcript variants encoding different isoforms have been found for
            this gene.[provided by RefSeq, Jul 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF079529.2, AL831924.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264917.10/ ENSP00000264917.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-585               AC022414.8         88667-89251         c
            586-645             AC022422.5         58317-58376
            646-706             AC022422.5         71862-71922
            707-836             KF457931.1         245-374             c
            837-896             AC022422.5         75321-75380
            897-954             AC022422.5         77725-77782
            955-1043            AC022422.5         83550-83638
            1044-1122           AC022422.5         91176-91254
            1123-1263           AC022422.5         95742-95882
            1264-1352           AC022422.5         97388-97476
            1353-1413           AC022422.5         99669-99729
            1414-1456           AC022422.5         146571-146613
            1457-1534           AC022422.5         151043-151120
            1535-1611           AC022422.5         153704-153780
            1612-1776           KC877015.1         240-404             c
            1777-1822           AC022422.5         157999-158044
            1823-1958           AC022422.5         158423-158558
            1959-2157           AC022422.5         159434-159632
            2158-2375           AC010234.5         139088-139305       c
            2376-2496           AC010234.5         137647-137767       c
            2497-2664           AC010234.5         135546-135713       c
            2665-2794           AC010234.5         131638-131767       c
            2795-4606           AC010234.5         129278-131089       c
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.3"
     gene            1..4606
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /note="phosphodiesterase 8B"
                     /db_xref="GeneID:8622"
                     /db_xref="HGNC:HGNC:8794"
                     /db_xref="MIM:603390"
     exon            1..585
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..2904
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /EC_number="3.1.4.53"
                     /note="isoform 1 is encoded by transcript variant 1; 3',5'
                     cyclic nucleotide phosphodiesterase 8B; high affinity
                     cAMP-specific and IBMX-insensitive 3',5'-cyclic
                     phosphodiesterase 8B; cell proliferation-inducing gene 22
                     protein"
                     /codon_start=1
                     /product="high affinity cAMP-specific and IBMX-insensitive
                     3',5'-cyclic phosphodiesterase 8B isoform 1"
                     /protein_id="NP_003710.1"
                     /db_xref="CCDS:CCDS4037.1"
                     /db_xref="GeneID:8622"
                     /db_xref="HGNC:HGNC:8794"
                     /db_xref="MIM:603390"
                     /translation="MGCAPSIHVSQSGVIYCRDSDESSSPRQTTSVSQGPAAPLPGLF
                     VQTDAADAIPPSRASGPPSVARVRRARTELGSGSSAGSAAPAATTSRGRRRHCCSSAE
                     AETQTCYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCN
                     IARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHTVILAVVSRVS
                     DDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALD
                     HCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTC
                     IKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDNNKQIHK
                     IHRDSGDNSQTEPHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIE
                     APITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGL
                     MTDGLRRLSGNEYVFTKNVHQSHSHLAMPITINDVPPCISQLLDNEESWDFNIFELEA
                     ITHKRPLVYLGLKVFSRFGVCEFLNCSETTLRAWFQVIEANYHSSNAYHNSTHAADVL
                     HATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTA
                     VLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFV
                     NSINKPMAAEIEGSDCECNPAGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGR
                     ISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPA
                     LMQHLADNYKHWKTLDDLKCKSLRLPSDS"
     exon            586..645
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            646..836
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            837..896
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            897..954
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1043
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1122
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1263
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1352
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1413
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1456
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1534
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1611
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1776
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1822
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1958
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2157
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2375
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2496
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2664
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2665..2794
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2795..4606
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4526..4531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /note="hexamer: ACTAAA"
     polyA_site      4557
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /note="major polyA site"
     polyA_site      4606
                     /gene="PDE8B"
                     /gene_synonym="ADSD; PPNAD3"
                     /note="major polyA site"
ORIGIN      
        1 gtgtatgcgc gctcccccgc tcggggagga agatggccca aaagggaaag ttggggtgac
       61 gcgcgcggtc cccggaggct cggcgggggg caccgcggcc agcccgacgg agcggcggac
      121 acacaggccg gggggcgcgc agtccgggcg ccgccgcggc cgccccctca ctgcaggtgg
      181 cagcgggtgc gctgggtccc ggcggccgcg ggcgcgggcg ggcgcgcggg ggagcccggc
      241 cgagggatgg gctgcgcccc cagcatccat gtctcgcaga gcggcgtgat ctactgccgg
      301 gactcggacg agtccagctc gccccgccag accaccagcg tgtcgcaggg cccggcggca
      361 cccctgcccg gcctcttcgt ccagaccgac gccgccgacg ccatcccccc gagccgcgcg
      421 tcgggacccc ccagcgtagc ccgcgtccgc agggcccgca ccgagctggg cagcggtagc
      481 agcgcgggtt ccgcagcccc cgccgcgacc accagcaggg gccggaggcg ccactgctgc
      541 agcagcgccg aggccgagac tcagacctgc tacaccagcg tgaagcaggt gtcttctgcg
      601 gaggtgcgca tcgggcccat gagactgacg caggacccta ttcaggtttt gctgatcttt
      661 gcaaaggaag atagtcagag cgatggcttc tggtgggcct gcgacagagc tggttataga
      721 tgcaatattg ctcggactcc agagtcagcc cttgaatgct ttcttgataa gcatcatgaa
      781 attattgtaa ttgatcatag acaaactcag aacttcgatg cagaagcagt gtgcaggtcg
      841 atccgggcca caaatccctc cgagcacacg gtgatcctcg cagtggtttc gcgagtatcg
      901 gatgaccatg aagaggcgtc agtccttcct cttctccacg caggcttcaa caggagattt
      961 atggagaata gcagcataat tgcttgctat aatgaactga ttcaaataga acatggggaa
     1021 gttcgctccc agttcaaatt acgggcctgt aattcagtgt ttacagcatt agatcactgt
     1081 catgaagcca tagaaataac aagcgatgac cacgtgattc agtatgtcaa cccagccttc
     1141 gaaaggatga tgggctacca caaaggtgag ctcctgggaa aagaactcgc tgatctgccc
     1201 aaaagcgata agaaccgggc agaccttctc gacaccatca atacatgcat caagaaggga
     1261 aaggagtggc agggggttta ctatgccaga cggaaatccg gggacagcat ccaacagcac
     1321 gtgaagatca ccccagtgat tggccaagga gggaaaatta ggcattttgt ctcgctcaag
     1381 aaactgtgtt gtaccactga caataataag cagattcaca agattcatcg tgattcagga
     1441 gacaattctc agacagagcc tcattcattc agatataaga acaggaggaa agagtccatt
     1501 gacgtgaaat cgatatcatc tcgaggcagt gatgcaccaa gcctgcagaa tcgtcgctat
     1561 ccgtccatgg cgaggatcca ctccatgacc atcgaggctc ccatcacaaa ggttataaat
     1621 ataatcaatg cagcccaaga aaacagccca gtcacagtag cggaagcctt ggacagagtt
     1681 ctagagattt tacggaccac agaactgtac tcccctcagc tgggtaccaa agatgaagat
     1741 ccccacacca gtgatcttgt tggaggcctg atgactgacg gcttgagaag actgtcagga
     1801 aacgagtatg tgtttactaa gaatgtgcac cagagtcaca gtcaccttgc aatgccaata
     1861 accatcaatg atgttccccc ttgtatctct caattacttg ataatgagga gagttgggac
     1921 ttcaacatct ttgaattgga agccattacg cataaaaggc cattggttta tctgggctta
     1981 aaggtcttct ctcggtttgg agtatgtgaa tttttaaact gttctgaaac cactcttcgg
     2041 gcctggttcc aagtgatcga agccaactac cactcttcca atgcctacca caactccacc
     2101 catgctgccg acgtcctgca cgccaccgct ttctttcttg gaaaggaaag agtaaaggga
     2161 agcctcgatc agttggatga ggtggcagcc ctcattgctg ccacagtcca tgacgtggat
     2221 cacccgggaa ggaccaactc tttcctctgc aatgcaggca gtgagcttgc tgtgctctac
     2281 aatgacactg ctgttctgga gagtcaccac accgccctgg ccttccagct cacggtcaag
     2341 gacaccaaat gcaacatttt caagaatatt gacaggaacc attatcgaac gctgcgccag
     2401 gctattattg acatggtttt ggcaacagag atgacaaaac actttgaaca tgtgaataag
     2461 tttgtgaaca gcatcaacaa gccaatggca gctgagattg aaggcagcga ctgtgaatgc
     2521 aaccctgctg ggaagaactt ccctgaaaac caaatcctga tcaaacgcat gatgattaag
     2581 tgtgctgacg tggccaaccc atgccgcccc ttggacctgt gcattgaatg ggctgggagg
     2641 atctctgagg agtattttgc acagactgat gaagagaaga gacagggact acctgtggtg
     2701 atgccagtgt ttgaccggaa tacctgtagc atccccaagt ctcagatctc tttcattgac
     2761 tacttcataa cagacatgtt tgatgcttgg gatgcctttg cacatctgcc agccctgatg
     2821 caacatttgg ctgacaacta caaacactgg aagacactag atgacctaaa gtgcaaaagt
     2881 ttgaggcttc catctgacag ctaaagccaa gccacagagg gggcctcttg accgacaaag
     2941 gacactgtga atcacagtag cgtaaacgag aggccttcct ttctaatgac aatgacaggt
     3001 attggtgaag gagctaatgt ttaatatttg accttgaatc attcaagtcc ccaaatttca
     3061 ttcttagaaa gttatgttcc atgaagaaaa atatatgttc ttttgaatac ttaatgacag
     3121 aacaaatact tggcaaactc ctttgctctg ctgtcatcct gtgtaccctt gtcaatccat
     3181 ggagctggtt cactgtaact agcaggccac aggaagcaaa gccttggtgc ctgtgagctc
     3241 atctcccagg atggtgacta agtagcttag ctagtgatca gctcatcctt taccataaaa
     3301 gtcatcattg ctgtttagct tgactgtttt cctcaagaac atcgatctga aggattcata
     3361 aggagcttat ctgaacagat ttatctaaga aaaaaaaaaa acgacataaa ataagtgaaa
     3421 caactaggac caaattacag ataaactagt tagcttcaca gcctctatgg ctacatggtt
     3481 cttctggccg atggtatgac acctaagtta gaacacagcc ttggctggtg ggtgccctct
     3541 ctagactggt atcagcagcc tgtgtaaccc ctttcctgta aaaggggttc atcttaacaa
     3601 agtcatccat gatgagggaa aaagtggcat ttcatttttg gggaatccat gagcttcctt
     3661 tatttctggc tcacagaggc agccacgagg cactacacca agtattatat aaaagccatt
     3721 aaatttgaat gcccttggac aagcttttct taaaaaaaaa aaaaaaaagt ttatatacat
     3781 gtttaaaatt tttattaaaa tccaaatttt ctgggtgtaa gcccatgcag tatgttgtgc
     3841 catgcaccat ctatacgtaa tattttggga ggggataggg gaagtttcaa ggttcagata
     3901 tttttaacca gtctatattt gaaatcatgc cattagcttt aacaatgtta aaattgtgtg
     3961 gtgtaaatat atcagaaaat aggcatatgg ggaggcagta aatactattt taagctatta
     4021 attgtatgct aaaagcttct aaagcacaag tgcatatttt tatactgctc ttttcacaac
     4081 tccctgtgat ctacataaag tcagactcga gattttcctt tctcttttac cagctgatcc
     4141 ttcctatgta ttataaatat tagactccgt aaacaaactt catttttctt tctctgtact
     4201 tcatgctgca tttttaaaag gtataaacaa acagagactg aattaattga atcagtaatt
     4261 acttttttcc aatacaaatt ggaatgcaga atacttggaa cttgtacatg gggaaaaaat
     4321 gacttatcac tacataatgt gccaatgttt ttttctatgt tttgtaccaa aaatggaaaa
     4381 atatgacaat tccatgcaaa gaaatgtatc taaattattt ttgttagatg ataagagaac
     4441 ttgcttggtc aggacagcag ccagctttgt attgtaatgc tatattatgt aaatgtgatg
     4501 aaaatgtttt tgtgaagtac ttgtaactaa attcctacag ccatttttca ctcccaacag
     4561 ctgtttttat tttacctctt tggcctaaag ttttcataat ttcaaa
//
