LOCUS       NM_002605               4036 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 1,
            mRNA.
ACCESSION   NM_002605
VERSION     NM_002605.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Qasim,H., Rajaei,M., Xu,Y., Reyes-Alcaraz,A., Abdelnasser,H.Y.,
            Stewart,M.D., Lahiri,S.K., Wehrens,X.H.T. and McConnell,B.K.
  TITLE     AKAP12 Upregulation Associates With PDE8A to Accelerate Cardiac
            Dysfunction
  JOURNAL   Circ Res 134 (8), 1006-1022 (2024)
   PUBMED   38506047
  REMARK    GeneRIF: AKAP12 Upregulation Associates With PDE8A to Accelerate
            Cardiac Dysfunction.
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Mukherjee,S., Roy,S., Mukherjee,S., Harikishore,A., Bhunia,A. and
            Mandal,A.K.
  TITLE     14-3-3 interaction with phosphodiesterase 8A sustains PKA signaling
            and downregulates the MAPK pathway
  JOURNAL   J Biol Chem 300 (3), 105725 (2024)
   PUBMED   38325743
  REMARK    GeneRIF: 14-3-3 interaction with phosphodiesterase 8A sustains PKA
            signaling and downregulates the MAPK pathway.
REFERENCE   3  (bases 1 to 4036)
  AUTHORS   Campolo,F., Capponi,C., Tarsitano,M.G., Tenuta,M., Pozza,C.,
            Gianfrilli,D., Magliocca,F., Venneri,M.A., Vicini,E., Lenzi,A.,
            Isidori,A.M. and Barbagallo,F.
  TITLE     cAMP-specific phosphodiesterase 8A and 8B isoforms are
            differentially expressed in human testis and Leydig cell tumor
  JOURNAL   Front Endocrinol (Lausanne) 13, 1010924 (2022)
   PUBMED   36277728
  REMARK    GeneRIF: cAMP-specific phosphodiesterase 8A and 8B isoforms are
            differentially expressed in human testis and Leydig cell tumor.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4036)
  AUTHORS   Turner,M.J., Sato,Y., Thomas,D.Y., Abbott-Banner,K. and
            Hanrahan,J.W.
  TITLE     Phosphodiesterase 8A Regulates CFTR Activity in Airway Epithelial
            Cells
  JOURNAL   Cell Physiol Biochem 55 (6), 784-804 (2021)
   PUBMED   34936285
  REMARK    GeneRIF: Phosphodiesterase 8A Regulates CFTR Activity in Airway
            Epithelial Cells.
REFERENCE   5  (bases 1 to 4036)
  AUTHORS   Salvetat,N., Chimienti,F., Cayzac,C., Dubuc,B., Checa-Robles,F.,
            Dupre,P., Mereuze,S., Patel,V., Genty,C., Lang,J.P., Pujol,J.F.,
            Courtet,P. and Weissmann,D.
  TITLE     Phosphodiesterase 8A to discriminate in blood samples depressed
            patients and suicide attempters from healthy controls based on
            A-to-I RNA editing modifications
  JOURNAL   Transl Psychiatry 11 (1), 255 (2021)
   PUBMED   33931591
  REMARK    GeneRIF: Phosphodiesterase 8A to discriminate in blood samples
            depressed patients and suicide attempters from healthy controls
            based on A-to-I RNA editing modifications.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4036)
  AUTHORS   Gamanuma,M., Yuasa,K., Sasaki,T., Sakurai,N., Kotera,J. and
            Omori,K.
  TITLE     Comparison of enzymatic characterization and gene organization of
            cyclic nucleotide phosphodiesterase 8 family in humans
  JOURNAL   Cell Signal 15 (6), 565-574 (2003)
   PUBMED   12681444
  REMARK    GeneRIF: Comparison of enzymatic characterization and gene
            organization of PDE8B and PDE8A.
REFERENCE   7  (bases 1 to 4036)
  AUTHORS   Bass,B.L.
  TITLE     RNA editing by adenosine deaminases that act on RNA
  JOURNAL   Annu Rev Biochem 71, 817-846 (2002)
   PUBMED   12045112
  REMARK    Review article
REFERENCE   8  (bases 1 to 4036)
  AUTHORS   Wang,P., Wu,P., Egan,R.W. and Billah,M.M.
  TITLE     Human phosphodiesterase 8A splice variants: cloning, gene
            organization, and tissue distribution
  JOURNAL   Gene 280 (1-2), 183-194 (2001)
   PUBMED   11738832
REFERENCE   9  (bases 1 to 4036)
  AUTHORS   Glavas,N.A., Ostenson,C., Schaefer,J.B., Vasta,V. and Beavo,J.A.
  TITLE     T cell activation up-regulates cyclic nucleotide phosphodiesterases
            8A1 and 7A3
  JOURNAL   Proc Natl Acad Sci U S A 98 (11), 6319-6324 (2001)
   PUBMED   11371644
REFERENCE   10 (bases 1 to 4036)
  AUTHORS   Fisher,D.A., Smith,J.F., Pillar,J.S., St Denis,S.H. and Cheng,J.B.
  TITLE     Isolation and characterization of PDE8A, a novel human
            cAMP-specific phosphodiesterase
  JOURNAL   Biochem Biophys Res Commun 246 (3), 570-577 (1998)
   PUBMED   9618252
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087468.11, BC060762.1 and
            AF388183.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002605.2.
            
            Summary: The protein encoded by this gene belongs to the cyclic
            nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This
            PDE hydrolyzes the second messenger, cAMP, which is a regulator and
            mediator of a number of cellular responses to extracellular
            signals. Thus, by regulating the cellular concentration of cAMP,
            this protein plays a key role in many important physiological
            processes. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene.[provided by
            RefSeq, Jul 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036190.1, SRR1803613.60208.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394553.6/ ENSP00000378056.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               AC087468.11        68465-68582         c
            119-641             BC060762.1         64-586
            642-1182            AF388183.1         517-1057
            1183-4036           BC060762.1         1128-3981
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.3"
     gene            1..4036
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /note="phosphodiesterase 8A"
                     /db_xref="GeneID:5151"
                     /db_xref="HGNC:HGNC:8793"
                     /db_xref="MIM:602972"
     exon            1..493
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..2797
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /EC_number="3.1.4.53"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cAMP-specific cyclic nucleotide phosphodiesterase 8A; high
                     affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic
                     phosphodiesterase 8A"
                     /codon_start=1
                     /product="high affinity cAMP-specific and IBMX-insensitive
                     3',5'-cyclic phosphodiesterase 8A isoform 1"
                     /protein_id="NP_002596.1"
                     /db_xref="CCDS:CCDS10336.1"
                     /db_xref="GeneID:5151"
                     /db_xref="HGNC:HGNC:8793"
                     /db_xref="MIM:602972"
                     /translation="MGCAPSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQKTAALPR
                     TRGAGLLESELRDGSGKKVAVADVQFGPMRFHQDQLQVLLVFTKEDNQCNGFCRACEK
                     AGFKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQLDAEALCRSIRSSKLSENTVIVG
                     VVRRVDREELSVMPFISAGFTRRYVENPNIMACYNELLQLEFGEVRSQLKLRACNSVF
                     TALENSEDAIEITSEDRFIQYANPAFETTMGYQSGELIGKELGEVPINEKKADLLDTI
                     NSCIRIGKEWQGIYYAKKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKAEK
                     ISECVQSDTHTDNQTGKHKDRRKGSLDVKAVASRATEVSSQRRHSSMARIHSMTIEAP
                     ITKVINIINAAQESSPMPVTEALDRVLEILRTTELYSPQFGAKDDDPHANDLVGGLMS
                     DGLRRLSGNEYVLSTKNTQMVSSNIITPISLDDVPPRIARAMENEEYWDFDIFELEAA
                     THNRPLIYLGLKMFARFGICEFLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLH
                     ATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAV
                     LESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVN
                     SINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEW
                     AARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVD
                     LPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE"
     exon            494..550
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            551..741
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            742..798
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            799..853
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            854..942
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1021
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1159
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1248
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1300
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1343
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1421
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1492
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1657
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1706
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1842
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..2041
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2259
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2392
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2560
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2690
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..4036
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3291..3296
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /note="hexamer: ATTAAA"
     polyA_site      3321
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
     regulatory      4013..4018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /note="hexamer: ATTAAA"
     polyA_site      4036
                     /gene="PDE8A"
                     /gene_synonym="HsT19550"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgagg cgagcccggc gatgtgagag gcggccgtcg gctcctgcct caggaagggc
       61 atgttcggag gggcggcctc ggcacgccac ccgcctaagc gcccccttcc caccgcagcc
      121 gccgccgccg cagcgcccgc accgcgataa aaggggcggc cgcgtttcct gacgcgagat
      181 ccgcgctcgc cgccgcccgc ccaggcggcg atgacacggc gcccgcggcg gcccggaggc
      241 gccgggtggg ccgtttgctg accggatcgc ggctacccgc cagcgtgtcc gcggcgccgc
      301 cgccagcatg ggctgtgccc cgagcatcca catttccgag cgcctggtgg ccgaggacgc
      361 gcctagcccc gcggcaccgc cgctgtcgtc cggcgggccg cgcctcccgc agggccagaa
      421 gacggccgcc ttgccccgga cccgcggcgc cggcctcttg gagtcggagc ttcgcgacgg
      481 cagcggcaag aaggtagcag tagctgatgt gcagtttggc cccatgagat ttcatcaaga
      541 tcaacttcag gtacttttag tgtttaccaa agaagataac caatgtaatg gattctgcag
      601 ggcatgtgaa aaagcagggt ttaagtgtac agttaccaag gaggctcagg ctgtccttgc
      661 ctgtttcctg gacaaacatc atgacattat catcatagac cacagaaatc ctcgacagct
      721 ggatgcagag gcactgtgca ggtctatcag atcatcaaaa ctctcagaaa acacagttat
      781 tgttggtgta gtacgcaggg tggatagaga agagttgtcc gtaatgcctt tcatttctgc
      841 tggatttaca aggaggtatg tagaaaaccc caacatcatg gcctgctaca atgaactgct
      901 ccagctggag tttggagagg tgcgatcaca actgaaactc agggcttgta actcagtatt
      961 cactgcatta gaaaacagtg aagatgcaat tgaaattaca agcgaagacc gttttataca
     1021 gtatgcaaat cctgcatttg aaacaacaat gggctatcag tcaggtgaat taatagggaa
     1081 ggagttagga gaagtgccta taaatgaaaa aaaggctgac ttgctcgata ctataaattc
     1141 atgcatcagg ataggcaagg agtggcaagg aatttactat gccaaaaaga aaaacggaga
     1201 taatatacaa caaaatgtga agataatacc tgtcattgga cagggaggaa aaattagaca
     1261 ctatgtgtcc attatcagag tgtgcaatgg caacaataag gctgagaaaa tatccgaatg
     1321 tgttcagtct gacactcata cagataatca gacaggcaaa cataaagaca ggagaaaagg
     1381 ctcactagac gtcaaagctg ttgcctcccg tgcaactgaa gtttccagcc agagacgaca
     1441 ctcttccatg gcccggatac attccatgac aattgaggcg cccatcacca aggtaatcaa
     1501 tattatcaat gctgcccagg aaagtagtcc catgcctgtg acagaagccc tagaccgtgt
     1561 gctggaaatt ctaagaacca ctgagttata ttcaccacag tttggtgcta aagatgatga
     1621 tccccatgcc aatgaccttg ttgggggctt aatgtctgat ggtttgcgaa gactatcagg
     1681 gaatgaatat gttctttcaa caaaaaacac tcaaatggtt tcaagcaata taatcactcc
     1741 catctccctt gatgatgtcc caccacggat agctcgggcc atggaaaatg aggaatactg
     1801 ggactttgat atttttgaac tggaggctgc cacccacaat aggcctttga tttatcttgg
     1861 tctcaaaatg tttgctcgct ttggaatctg tgaattctta cactgctccg agtcaacgct
     1921 aagatcatgg ttacaaatta tcgaagccaa ttatcattcc tccaatccct accacaattc
     1981 tacacattct gctgatgtgc ttcatgccac tgcctatttt ctctccaagg agaggataaa
     2041 ggaaacttta gatccaattg atgaggtcgc tgcactcatc gcagccacca ttcatgatgt
     2101 ggatcaccct gggagaacca actccttcct gtgtaatgct ggaagtgagc tggccatttt
     2161 gtacaatgac actgctgtgc tggagagcca ccatgcggcc ttggccttcc agctgaccac
     2221 tggagatgat aaatgcaata tatttaaaaa catggagagg aatgattatc ggacactgcg
     2281 ccaggggatt atcgacatgg tcttagccac agaaatgaca aagcactttg agcatgtcaa
     2341 caaatttgtc aacagcatca acaaaccctt ggcaacacta gaagaaaatg gggaaactga
     2401 taaaaaccag gaagtgataa acactatgct taggactcca gagaaccgga ccctaatcaa
     2461 acgaatgctg attaaatgtg ctgatgtgtc caatccctgc cgacccctgc agtactgcat
     2521 cgagtgggct gcacgcattt cggaagaata tttttctcag actgatgaag agaagcagca
     2581 gggcttacct gtggtgatgc cagtgtttga cagaaatacc tgcagcatcc ccaaatccca
     2641 aatctctttc attgattact tcatcacaga catgtttgat gcttgggatg cctttgtaga
     2701 cctgcctgat ttaatgcagc atcttgacaa caactttaaa tactggaaag gactggacga
     2761 aatgaagctg cggaacctcc gaccacctcc tgaatagtgg gagacaccac ccagagccct
     2821 gaagctttgt tccttcggtc atttggaatt cctgagggca gccagagctc cttggtcctt
     2881 tcagtactag gcagaacagc ccccgatctg catagcctgt gaaagcccac ggggacatca
     2941 gtaaccttct gcagccacca tccaatgcca ttactgtcaa gtgagacttg gccactgtag
     3001 cctgggcctg ctgcaggagc tcttcagaaa ggcacatgag gaccacggtt tgcctcagtt
     3061 tctggtaaaa cacaaggtct ggagtgcccc tgcaaagggt attgatggac ttcctgccag
     3121 tgacagagca tgtctattgc aaacaattct ctcagttacg ttcagcactt aagaacggct
     3181 aatggcaata ggatctttag caactttttc acatcataga aggtgcaatc gctcacttgg
     3241 gaacactact gagagtgact tctcttttaa aattgagtag cagatgaaaa attaaaattt
     3301 gaacttgatt attaatatca attaaaatgt tttatttatt ttattaaaag ctcaatattt
     3361 tctatgaatt caaaaatact tcagagccaa agccaacttc aaataccgtg accaaattta
     3421 catgattcat attcattatg cattacttgg tatacagact tattttcata atgcaaatta
     3481 ataaaatgac acttttactg cactatagaa atattcatgt atgttaaact tttctgattg
     3541 aggctaactg gaaaaagctg gggtcgtatt ctaagtgcta aagaaggctg cttctactgt
     3601 atagaaccca gggctctgaa acagctctag ccgcctaatg cacttcacag gtaactcccc
     3661 aaggtaaaac tagactctct tgttggttcg caaagaaaag ttaggactta acactttttt
     3721 ctaaaatttt ataattcaat ttccaaaagt ctactctatt ttatactgtt tctacaaaat
     3781 attccttata aaaacaaaga acaaaaattg aatatttaat gaattgacat tttataacca
     3841 acctgttttt atctacggtg ggaatctttg atgccagaaa tttataaaga ggttctgtat
     3901 cttcacacct tgaataagca taataccata aaaaatgaca cttgacatgt caatgtattt
     3961 gtcatttcat tttaaactcg tatttgtggt ttttttccca gataaaaatg aaattaaacc
     4021 atttcttttt aagaaa
//
