LOCUS       NM_001242318            6961 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens phosphodiesterase 7A (PDE7A), transcript variant 3,
            mRNA.
ACCESSION   NM_001242318
VERSION     NM_001242318.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Aftanas,L.I., Filimonova,E.A., Anisimenko,M.S., Berdyugina,D.A.,
            Rezakova,M.V., Simutkin,G.G., Bokhan,N.A., Ivanova,S.A.,
            Danilenko,K.V. and Lipina,T.V.
  TITLE     The habenular volume and PDE7A allelic polymorphism in major
            depressive disorder: preliminary findings
  JOURNAL   World J Biol Psychiatry 24 (3), 223-232 (2023)
   PUBMED   35673941
  REMARK    GeneRIF: The habenular volume and PDE7A allelic polymorphism in
            major depressive disorder: preliminary findings.
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Zhang,X. and Dong,S.
  TITLE     Circ_0091702 relieves lipopolysaccharide (LPS)-induced cell injury
            by regulating the miR-182/PDE7A axis in sepsis
  JOURNAL   Biosci Biotechnol Biochem 85 (9), 1962-1970 (2021)
   PUBMED   34077501
  REMARK    GeneRIF: Circ_0091702 relieves lipopolysaccharide (LPS)-induced
            cell injury by regulating the miR-182/PDE7A axis in sepsis.
REFERENCE   3  (bases 1 to 6961)
  AUTHORS   Hao,L., Yun,Y., Liang,R. and Yuan,G.
  TITLE     Long non-coding RNA TUSC7 suppressed colorectal cancer progression
            via regulation of miR-23b/PDE7A Axis
  JOURNAL   Clin Invest Med 43 (4), E35-E43 (2020)
   PUBMED   33370523
  REMARK    GeneRIF: Long non-coding RNA TUSC7 suppressed colorectal cancer
            progression via regulation of miR-23b/PDE7A Axis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6961)
  AUTHORS   Morales-Garcia,J.A., Alonso-Gil,S., Santos,A. and Perez-Castillo,A.
  TITLE     Phosphodiesterase 7 Regulation in Cellular and Rodent Models of
            Parkinson's Disease
  JOURNAL   Mol Neurobiol 57 (2), 806-822 (2020)
   PUBMED   31473904
  REMARK    GeneRIF: Phosphodiesterase 7 Regulation in Cellular and Rodent
            Models of Parkinson's Disease.
REFERENCE   5  (bases 1 to 6961)
  AUTHORS   de Medeiros,A.S., Wyman,A.R., Alaamery,M.A., Allain,C., Ivey,F.D.,
            Wang,L., Le,H., Morken,J.P., Habara,A., Le,C., Cui,S., Lerner,A.
            and Hoffman,C.S.
  TITLE     Identification and characterization of a potent and
            biologically-active PDE4/7 inhibitor via fission yeast-based assays
  JOURNAL   Cell Signal 40, 73-80 (2017)
   PUBMED   28867658
  REMARK    GeneRIF: Here we describe a compound, BC54, that is a selective
            inhibitor of enzymes from the cAMP-specific PDE4 and PDE7 families.
            Consistent with the biological effect of other PDE4 and PDE7
            inhibitors, BC54 displays potent anti-inflammatory properties and
            is superior to a combination of rolipram (a PDE4 inhibitor) and
            BRL50481 (a PDE7A inhibitor) for inducing apoptosis in chronic
            lymphocytic leukemia (CLL) cells
REFERENCE   6  (bases 1 to 6961)
  AUTHORS   Han,P., Fletcher,C.F., Copeland,N.G., Jenkins,N.A., Yaremko,L.M.
            and Michaeli,T.
  TITLE     Assignment of the mouse Pde7A gene to the proximal region of
            chromosome 3 and of the human PDE7A gene to chromosome 8q13
  JOURNAL   Genomics 48 (2), 275-276 (1998)
   PUBMED   9521885
REFERENCE   7  (bases 1 to 6961)
  AUTHORS   Han,P., Zhu,X. and Michaeli,T.
  TITLE     Alternative splicing of the high affinity cAMP-specific
            phosphodiesterase (PDE7A) mRNA in human skeletal muscle and heart
  JOURNAL   J Biol Chem 272 (26), 16152-16157 (1997)
   PUBMED   9195912
REFERENCE   8  (bases 1 to 6961)
  AUTHORS   Bloom,T.J. and Beavo,J.A.
  TITLE     Identification and tissue-specific expression of PDE7
            phosphodiesterase splice variants
  JOURNAL   Proc Natl Acad Sci U S A 93 (24), 14188-14192 (1996)
   PUBMED   8943082
REFERENCE   9  (bases 1 to 6961)
  AUTHORS   Milatovich,A., Bolger,G., Michaeli,T. and Francke,U.
  TITLE     Chromosome localizations of genes for five cAMP-specific
            phosphodiesterases in man and mouse
  JOURNAL   Somat Cell Mol Genet 20 (2), 75-86 (1994)
   PUBMED   8009369
REFERENCE   10 (bases 1 to 6961)
  AUTHORS   Michaeli,T., Bloom,T.J., Martins,T., Loughney,K., Ferguson,K.,
            Riggs,M., Rodgers,L., Beavo,J.A. and Wigler,M.
  TITLE     Isolation and characterization of a previously undetected human
            cAMP phosphodiesterase by complementation of cAMP
            phosphodiesterase-deficient Saccharomyces cerevisiae
  JOURNAL   J Biol Chem 268 (17), 12925-12932 (1993)
   PUBMED   8389765
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC100812.2, DB460103.1,
            DR000686.1, AK292680.1, AK057586.1, AW387924.1, AC055822.10,
            CN414361.1, N49050.1 and BM818331.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001242318.2.
            
            Summary: The protein encoded by this gene belongs to the cyclic
            nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This
            PDE hydrolyzes the second messenger, cAMP, which is a regulator and
            mediator of a number of cellular responses to extracellular
            signals. Thus, by regulating the cellular concentration of cAMP,
            this protein plays a key role in many important physiological
            processes. Alternatively spliced transcript variants encoding
            different isoforms have been described for this gene. [provided by
            RefSeq, Jul 2011].
            
            Transcript Variant: This variant (3, also called PDE7A1) represents
            the longer transcript, and encodes the longer isoform (c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292680.1, SRR14038192.661791.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000401827.8/ ENSP00000385632.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AC100812.2         78508-78837         c
            331-455             DB460103.1         1-125
            456-544             DR000686.1         350-438
            545-2094            AK292680.1         12-1561
            2095-3609           AK057586.1         1267-2781
            3610-3738           AW387924.1         357-485             c
            3739-3877           AC055822.10        84859-84997
            3878-4385           CN414361.1         1-508
            4386-4690           N49050.1           1-305               c
            4691-6909           AC055822.10        85811-88029
            6910-6961           BM818331.1         225-276
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.1"
     gene            1..6961
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /note="phosphodiesterase 7A"
                     /db_xref="GeneID:5150"
                     /db_xref="HGNC:HGNC:8791"
                     /db_xref="MIM:171885"
     exon            1..694
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     CDS             557..2005
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /EC_number="3.1.4.53"
                     /note="isoform c is encoded by transcript variant 3; high
                     affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A;
                     phosphodiesterase isozyme 7; TM22; high affinity
                     cAMP-specific PDE 1; cAMP-specific phosphodiesterase 7A;
                     high affinity 3',5'-cyclic-AMP phosphodiesterase 7A"
                     /codon_start=1
                     /product="high affinity 3',5'-cyclic-AMP phosphodiesterase
                     7A isoform c"
                     /protein_id="NP_001229247.1"
                     /db_xref="CCDS:CCDS56538.1"
                     /db_xref="GeneID:5150"
                     /db_xref="HGNC:HGNC:8791"
                     /db_xref="MIM:171885"
                     /translation="MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPNPRQL
                     SQRRGAISYDSSDQTALYIRMLGDVRVRSRAGFESERRGSHPYIDFRIFHSQSEIEVS
                     VSARNIRRLLSFQRYLRSSRFFRGTAVSNSLNILDDDYNGQAKCMLEKVGNWNFDIFL
                     FDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNPYHNAVHAA
                     DVTQAMHCYLKEPKLANSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYK
                     NTSVLENHHWRSAVGLLRESGLFSHLPLESRQQMETQIGALILATDISRQNEYLSLFR
                     SHLDRGDLCLEDTRHRHLVLQMALKCADICNPCRTWELSKQWSEKVTEEFFHQGDIEK
                     KYHLGVSPLCDRHTESIANIQIGFMTYLVEPLFTEWARFSNTRLSQTMLGHVGLNKAS
                     WKGLQREQSSSEDTDAAFELNSQLLPQENRLS"
     exon            695..755
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            756..839
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            840..991
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1055
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1151
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1252
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1384
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1476
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1621
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1718
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1799
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..6961
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3591..3596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /note="hexamer: AATAAA"
     polyA_site      3612
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /note="major polyA site"
     regulatory      6517..6522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /note="hexamer: ATTAAA"
     polyA_site      6546
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
     regulatory      6937..6942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
                     /note="hexamer: AATAAA"
     polyA_site      6961
                     /gene="PDE7A"
                     /gene_synonym="HCP1; PDE7"
ORIGIN      
        1 gtgaattacg ccgggctcgc gccggcggcg gagtgaagtc aggctccggg ggaggggagg
       61 agcaggactc cggcggcggc ggcggcggcg gcggcggcgg cccgagctgg tcgctgctcc
      121 tgagtcccgc gcgccctggg tctggccgct ggctccagct gtcttttctt gcactgcttt
      181 gctccgttcg tgctgcgctc gaactctgca gtccgggtac ctgctcctct cggccgagga
      241 gccccagggc ggcggggttc cctttccgac tttgcctgtg ctggccccgc ttgcaccccg
      301 tcccccctcg cctggagcga ggcgcgcgcc ggtcctggta attggcgccg cggccgccgc
      361 ggagccttcc cgccggcggc ccgagcggag ggctggggtt ggggaggcgt gagcgcggct
      421 tcccagcccg gggtcggtcc ccgcccgccc tgcccccgtc tcggcccgga gcccccgagc
      481 cgcggccccc tcccactgca ggggccggcg gccgcggcag ggcgggcgcc gcgcggaggc
      541 agggcgggcg tattcaatgg aagtgtgtta ccagctgccg gtactgcccc tggacaggcc
      601 ggtcccccag cacgtcctca gccgccgagg agccatcagc ttcagctcca gctccgctct
      661 cttcggctgc cccaatcccc ggcagctctc tcagaggcgt ggagctattt cctatgacag
      721 ttctgatcag actgcattat acattcgtat gctaggagat gtacgtgtaa ggagccgagc
      781 aggatttgaa tcagaaagaa gaggttctca cccatatatt gattttcgta ttttccactc
      841 tcaatctgaa attgaagtgt ctgtctctgc aaggaatatc agaaggctac taagtttcca
      901 gcgatatctt agatcttcac gcttttttcg tggtactgcg gtttcaaatt ccctaaacat
      961 tttagatgat gattataatg gacaagccaa gtgtatgctg gaaaaagttg gaaattggaa
     1021 ttttgatatc tttctatttg atagactaac aaatggaaat agtctagtaa gcttaacctt
     1081 tcatttattt agtcttcatg gattaattga gtacttccat ttagatatga tgaaacttcg
     1141 tagattttta gttatgattc aagaagatta ccacagtcaa aatccttacc ataacgcagt
     1201 ccacgctgcg gatgttactc aggccatgca ctgttactta aaggaaccta agcttgccaa
     1261 ttctgtaact ccttgggata tcttgctgag cttaattgca gctgccactc atgatctgga
     1321 tcatccaggt gttaatcaac ctttccttat taaaactaac cattacttgg caactttata
     1381 caagaatacc tcagtactgg aaaatcacca ctggagatct gcagtgggct tattgagaga
     1441 atcaggctta ttctcacatc tgccattaga aagcaggcaa caaatggaga cacagatagg
     1501 tgctctgata ctagccacag acatcagtcg ccagaatgag tatctgtctt tgtttaggtc
     1561 ccatttggat agaggtgatt tatgcctaga agacaccaga cacagacatt tggttttaca
     1621 gatggctttg aaatgtgctg atatttgtaa cccatgtcgg acgtgggaat taagcaagca
     1681 gtggagtgaa aaagtaacgg aggaattctt ccatcaagga gatatagaaa aaaaatatca
     1741 tttgggtgtg agtccacttt gcgatcgtca cactgaatct attgccaaca tccagattgg
     1801 ttttatgact tacctagtgg agcctttatt tacagaatgg gccaggtttt ccaatacaag
     1861 gctatcccag acaatgcttg gacacgtggg gctgaataaa gccagctgga agggactgca
     1921 gagagaacag tcgagcagtg aggacactga tgctgcattt gagttgaact cacagttatt
     1981 acctcaggaa aatcggttat cataaccccc agaaccagtg ggacaaactg cctcctggag
     2041 gtttttagaa atgtgaaatg gggtcttgag gtgagagaac ttaactcttg actgccaagg
     2101 tttccaagtg agtgatgcca gccagcatta tttatttcca agatttcctc tgttggatca
     2161 tttgaaccca cttgttaatt gcaagacccg aacatacagc aatatgaatt tggctttcat
     2221 gtgaaacctt gaatatgcaa agcccagcag gagagaatcc gaaaggagta acaaaggaag
     2281 ttttgatatg tgccacgact ttttcaaagc atctaatctt caaaacgtga aacttgaatt
     2341 gttcagcaac aatctcttgg aatttaacca gtctgatgca acaatgtgta tcttgtacct
     2401 tccactaagt tctctctgag aaaatggaaa tgtgaagtgc ccagcctctg ctgcctctgg
     2461 caagacaatg tttacaaatc aactctgaaa atattggttc taaattgcct tggagcatga
     2521 ttgtgaagga accactcaaa caaatttaaa gatcaaactt tagactgcag ctctttcccc
     2581 ctggtttgcc tttttcttct ttggatgcca ccaaagcctc ccatttgcta tagttttatt
     2641 tcatgcactg gaaactgagc atttatcgta gagtaccgcc aagctttcac tccagtgccg
     2701 tttggcaatg caattttttt tagcaattag tttttaattt ggggtgggag gggaagaaca
     2761 ccaatgtcct agctgtatta tgattctgca gtgaagacat tgcatgttgt tttcactact
     2821 gtacacttga cctgcacatg cgagaaaaag gtggaatgtt taaaacacca taatcagctc
     2881 agggtatttg ccaatctgaa ataaaagtgg gatgggagag tgtgtccttc agatcaaggg
     2941 tactaaagtc cctttcgctg cagtgagtga gaggtatgtt gtgtgtgaat gtacggatgt
     3001 gtgtttgcgt gcatgtttgt gcatgtgtga ctgtgcatgt tatgtttctc catgtgggca
     3061 aagatttgaa atgtaagctt ttatttatta ttttagaatg tgacataatg agcagccaca
     3121 ctcgggggag gggaaggttg gtaggtaagc tgtaacagat tgctccagtt gccttaaact
     3181 atgcacatag ctaagtgacc aaacttcttg ttttgatttg aaaaaagtgc attgttttct
     3241 tgtccctccc tttgatgaaa cgttaccctt tgacgggcct tttgatgtga acagatgttt
     3301 tctaggacaa actataagga ctaattttaa acttcaaaca ttccactttt gtaatttgtt
     3361 ttaaattgtt ttatgtatag taagcacaac tgtaatctag ttttaagaga aaccggtgct
     3421 ttcttttagt tcatttgtat ttcccttgtt actgtaaaag actgtttatt aattgtttac
     3481 agtttgttgc aacagccatt ttcttgggag aaagcttgag tgtaaagcca tttgtaaaag
     3541 gctttgccat actcatttta atatgtgcct gttgctgtta acttttgatg aataaaaacc
     3601 tatcttttca tatttttatc tttgacattt accttataac tcaatctttt ggaaaacaca
     3661 atgctaaatt acaaggaaat caaagttagc ttcttggctc ttgtaattca tccctcagat
     3721 gtttactcag tccgagctta tctactaatg actcaccttg caagtgagta agcagaagcc
     3781 cagttagggc tgttggagtc ctgactaaca caggggagag gtcagctttg agccaggcct
     3841 tttgttccta cagtggtgaa ctgcataaga catcagcaag tcttgcaagt actaaaatac
     3901 tagttgtatt tatttctatc aatcattggc ctttttaaag tgataagaaa aggccactcc
     3961 actctacttg attgatatca gttgtaaaca aggtaccact gtgccattcc ctatatcttc
     4021 ccaggtccaa ctcattagaa gggatatggt gcaaacttct aaatcaaaca cattaacata
     4081 ttatttgtac agaagagtgt tgatgtccaa tagaaacaga atccaagcca cataattttc
     4141 ggttttctag tagccacatt tagaaaacat ggaattaatt ttagtaacat ctattccaat
     4201 acgtccaaaa tattgtatca ctttaaaatg tcaatataaa aacaattaga tgtttttaca
     4261 ttcttttggg gggtactaag tctttgaaat ccagtgtata ttgtgtactt acagcacatc
     4321 tcaatttgga ctagtcatat ctcaggcacc caatatccac atgtggctag tggctaccat
     4381 attggacagt gtgggtatag aggtctgagg gtagtagagg aatatggtca gctggtcact
     4441 ctcgtgagat ggccatacct tggcctatat ttaatgtgta cagaaagtct gataactcag
     4501 atccttaaaa attcaggccc actgtttcag tacatagaga gtggaactat catggcaaac
     4561 ccacattaac caagctcaca gtaactattt ttttaaaaca ctatcaatat tttaatttac
     4621 agtgagaaat gtaactatca attattctct ccctttaggc aaatcttcct gggaagccaa
     4681 attatgagca gctggtgagc atggattctg agcaagggaa gcaagttgcc tcgtgcaccc
     4741 atgtcccttt gggtgcacag aagacccatg tcttctgtga tattcatgtt tgtggaccag
     4801 ggtaccctct tcccctggaa ggtgctagag attctctgcc agtagttgct tctctacatc
     4861 agcttctgat tggcacatgc attaaataac gtcattacct caagccttgt ttcagttgag
     4921 cttacctggg ctccattctg cctgtataca tgattgcttc tgctctgatc cattgtttct
     4981 cttatagttt gaaactgaaa agatcatcct atttccacat tctcagggga actgtgggtg
     5041 tggctcccat ttcctgccta cacatataat aaacccattg aaaagttgtt ttattatgta
     5101 tatgtgggaa tcaccttcta tagccctttt gttttttttt ttttttgaga cagggtctcg
     5161 ctgctaacta ggctggagtg cagtggcatg atcactgcag cctccacctc ctaggctcaa
     5221 gcaatcctcc cacctcagcc tcctgagtgc caccacacca cccagctaat tttttttttt
     5281 tttttttttt ttttttttga gacagagcct tgctctgtcg cccaggctgg agtgcagtgc
     5341 cgcgatattg gctcactgca agttccgcct cccgggttca cgccattctc ctcattctcc
     5401 tgcctcagtc tcccaggtcg ctgggactac aggcgcccgc caccaggccc tgctaatttt
     5461 tttgtacttt attagtagag ttagggtttc accatgttag ccaggatggt ctcaatctcc
     5521 tgacctcgtg atccgcccgc ctcggcctcc catagtgctg ggattacagg cgtgagccac
     5581 cgtgcccggc cagctaattt tttaagaaca tttttgtggc cgggcatggt ggctcacacc
     5641 tgtaatccca gcactttggg aggctgaggt gggtggatca cctgaggtcg ggagttcgag
     5701 accagtctgg ccaacatggt gaaaccccat ctctactaaa aatgcaaaaa ttagctgggc
     5761 gtggtggcgc attcctgtaa tcccagctac tcgggaggct gaggcggaag aattgcttga
     5821 acccaggtgg cggaggttgc aaggagcaga gattgtgcta ctgcactcca gcctaggtga
     5881 cagagcaaga ctctgtctca aaaaaaaaaa aaaaactttt ttatagagac agggtctctc
     5941 tttgttggct tggctggtct tgaactcttg ggctcaagca gtcctgccac cgtggcctct
     6001 caaagtgctg ggattacagg tgtgagacac tgcatcaggc ctatagctcc acttaaatac
     6061 gacctatatt actggaatat ttttttctaa aaaattttag gaaacagtta aatatttaaa
     6121 agattacttt tttaaaaagc aagtaaatat tacagaacag aatgcaaaat ttattttaca
     6181 tgggagacta aaaattttca cacacatggc caccctttgg gcctcttggc tcaacatttc
     6241 aggggctgag ggagtctctg ctctaggagt aaaaggtcct tggagagaaa attttgacaa
     6301 gtcctaactt taaaaaagaa aaattggttc ctggtcaata tttgtactca ctgtaaatgt
     6361 gatggttggt aatgatactc tgtataaaag taaaaatcat attttatgtc ctttgcaaac
     6421 aaatatattt gtacctgagg tacagattag aggtgctagt aattccttga agattctgaa
     6481 aatgatcttt ttttccaaaa tgctttcttg gtttttatta aactttctat tttcttaaaa
     6541 ctctcacatc aggtatttcc agtgttttca agcatgtata aatattttaa tgtataaatg
     6601 ttgtttaaaa tcccctcaat cggtatctcc tgattgagaa aatacacagt gaccaatagc
     6661 taataggaat tcagtattgc tttcaaagaa atgtgcatat taatcactgt agcatctgca
     6721 tacatttgaa acacggagta atacaggtct gtgcaagaat tagcagcttg ttccttgcta
     6781 ataatgactt gtgtcatctg gaaggccccc aagagcccat ggtctacagg tggaatgacc
     6841 accacttggg agtgagctga tggagagcac ccagcaggtc tcctccatct tcctggttcc
     6901 tgtgctcacc atagggctgg tgtacattgg gccattaata aacgtttgtt gaacaaatta
     6961 a
//
