LOCUS       NM_000440               5645 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens phosphodiesterase 6A (PDE6A), transcript variant 1,
            mRNA.
ACCESSION   NM_000440
VERSION     NM_000440.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Kuehlewein,L., Strasser,T., Blumenstock,G., Stingl,K.,
            Fischer,M.D., Wilhelm,B., Zrenner,E., Wissinger,B., Kohl,S.,
            Weisschuh,N. and Zobor,D.
  CONSRTM   RD-CURE Consortium
  TITLE     Central Visual Function and Genotype-Phenotype Correlations in
            PDE6A-Associated Retinitis Pigmentosa
  JOURNAL   Invest Ophthalmol Vis Sci 63 (5), 9 (2022)
   PUBMED   35533076
  REMARK    GeneRIF: Central Visual Function and Genotype-Phenotype
            Correlations in PDE6A-Associated Retinitis Pigmentosa.
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Li,Y., Li,R., Dai,H. and Li,G.
  TITLE     Novel variants in PDE6A and PDE6B genes and its phenotypes in
            patients with retinitis pigmentosa in Chinese families
  JOURNAL   BMC Ophthalmol 22 (1), 27 (2022)
   PUBMED   35033039
  REMARK    GeneRIF: Novel variants in PDE6A and PDE6B genes and its phenotypes
            in patients with retinitis pigmentosa in Chinese families.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5645)
  AUTHORS   Cote,R.H., Gupta,R., Irwin,M.J. and Wang,X.
  TITLE     Photoreceptor Phosphodiesterase (PDE6): Structure, Regulatory
            Mechanisms, and Implications for Treatment of Retinal Diseases
  JOURNAL   Adv Exp Med Biol 1371, 33-59 (2022)
   PUBMED   34170501
  REMARK    GeneRIF: Photoreceptor Phosphodiesterase (PDE6): Structure,
            Regulatory Mechanisms, and Implications for Treatment of Retinal
            Diseases.
            Review article
REFERENCE   4  (bases 1 to 5645)
  AUTHORS   Kuehlewein,L., Zobor,D., Andreasson,S.O., Ayuso,C., Banfi,S.,
            Bocquet,B., Bernd,A.S., Biskup,S., Boon,C.J.F., Downes,S.M.,
            Fischer,M.D., Holz,F.G., Kellner,U., Leroy,B.P., Meunier,I.,
            Nasser,F., Rosenberg,T., Rudolph,G., Stingl,K., Thiadens,A.A.H.J.,
            Wilhelm,B., Wissinger,B., Zrenner,E., Kohl,S. and Weisschuh,N.
  CONSRTM   RD-CURE Consortium
  TITLE     Clinical Phenotype and Course of PDE6A-Associated Retinitis
            Pigmentosa Disease, Characterized in Preparation for a Gene
            Supplementation Trial
  JOURNAL   JAMA Ophthalmol 138 (12), 1241-1250 (2020)
   PUBMED   33057649
  REMARK    GeneRIF: Clinical Phenotype and Course of PDE6A-Associated
            Retinitis Pigmentosa Disease, Characterized in Preparation for a
            Gene Supplementation Trial.
REFERENCE   5  (bases 1 to 5645)
  AUTHORS   Khateb,S., Nassisi,M., Bujakowska,K.M., Mejecase,C., Condroyer,C.,
            Antonio,A., Foussard,M., Demontant,V., Mohand-Said,S., Sahel,J.A.,
            Zeitz,C. and Audo,I.
  TITLE     Longitudinal Clinical Follow-up and Genetic Spectrum of Patients
            With Rod-Cone Dystrophy Associated With Mutations in PDE6A and
            PDE6B
  JOURNAL   JAMA Ophthalmol 137 (6), 669-679 (2019)
   PUBMED   30998820
  REMARK    GeneRIF: Mutations in PDE6A and PDE6B accounted for 1.6% and 2.4%,
            respectively, in a cohort of French patients with rod-cone
            dystrophy (RCD)
REFERENCE   6  (bases 1 to 5645)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
REFERENCE   7  (bases 1 to 5645)
  AUTHORS   Catty,P., Pfister,C., Bruckert,F. and Deterre,P.
  TITLE     The cGMP phosphodiesterase-transducin complex of retinal rods.
            Membrane binding and subunits interactions
  JOURNAL   J Biol Chem 267 (27), 19489-19493 (1992)
   PUBMED   1326553
REFERENCE   8  (bases 1 to 5645)
  AUTHORS   Pittler,S.J. and Baehr,W.
  TITLE     The molecular genetics of retinal photoreceptor proteins involved
            in cGMP metabolism
  JOURNAL   Prog Clin Biol Res 362, 33-66 (1991)
   PUBMED   1672236
  REMARK    Review article
REFERENCE   9  (bases 1 to 5645)
  AUTHORS   Pittler,S.J., Baehr,W., Wasmuth,J.J., McConnell,D.G.,
            Champagne,M.S., vanTuinen,P., Ledbetter,D. and Davis,R.L.
  TITLE     Molecular characterization of human and bovine rod photoreceptor
            cGMP phosphodiesterase alpha-subunit and chromosomal localization
            of the human gene
  JOURNAL   Genomics 6 (2), 272-283 (1990)
   PUBMED   2155175
REFERENCE   10 (bases 1 to 5645)
  AUTHORS   Takemoto,D.J. and Cunnick,J.M.
  TITLE     Visual transduction in rod outer segments
  JOURNAL   Cell Signal 2 (2), 99-104 (1990)
   PUBMED   2169289
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M26061.1, BC035909.1,
            AC008427.9, AC008545.3 and BU734142.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000440.2.
            
            Summary: This gene encodes the cyclic-GMP (cGMP)-specific
            phosphodiesterase 6A alpha subunit, expressed in cells of the
            retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a
            heterotrimer composed of an alpha, beta, and two gamma subunits.
            cGMP is an important regulator of rod cell membrane current, and
            its dynamic concentration is established by phosphodiesterase 6A
            cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein
            is a subunit of a key phototransduction enzyme and participates in
            processes of transmission and amplification of the visual signal.
            Mutations in this gene have been identified as one cause of
            autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M26061.1, BC035909.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA1968832 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000255266.10/ ENSP00000255266.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               M26061.1           1-111
            112-2944            BC035909.1         99-2931
            2945-3846           AC008427.9         45019-45920         c
            3847-5245           AC008545.3         89095-90493         c
            5246-5642           BU734142.1         18-414              c
            5643-5645           AC008545.3         88695-88697         c
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..5645
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /note="phosphodiesterase 6A"
                     /db_xref="GeneID:5145"
                     /db_xref="HGNC:HGNC:8785"
                     /db_xref="MIM:180071"
     exon            1..594
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2703
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /EC_number="3.1.4.35"
                     /note="isoform 1 is encoded by transcript variant 1; rod
                     cGMP-specific 3',5'-cyclic phosphodiesterase subunit
                     alpha; cGMP phosphodiesterase alpha subunit; rod
                     photoreceptor cGMP phosphodiesterase alpha subunit; PDE
                     V-B1; GMP-PDE alpha; phosphodiesterase 6A, cGMP-specific,
                     rod, alpha; retinitis pigmentosa type 43"
                     /codon_start=1
                     /product="rod cGMP-specific 3',5'-cyclic phosphodiesterase
                     subunit alpha isoform 1"
                     /protein_id="NP_000431.2"
                     /db_xref="CCDS:CCDS4299.1"
                     /db_xref="GeneID:5145"
                     /db_xref="HGNC:HGNC:8785"
                     /db_xref="MIM:180071"
                     /translation="MGEVTAEEVEKFLDSNIGFAKQYYNLHYRAKLISDLLGAKEAAV
                     DFSNYHSPSSMEESEIIFDLLRDFQENLQTEKCIFNVMKKLCFLLQADRMSLFMYRTR
                     NGIAELATRLFNVHKDAVLEDCLVMPDQEIVFPLDMGIVGHVAHSKKIANVPNTEEDE
                     HFCDFVDILTEYKTKNILASPIMNGKDVVAIIMAVNKVDGSHFTKRDEEILLKYLNFA
                     NLIMKVYHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYS
                     VGLLDMTKQKEFFDVWPVLMGEVPPYSGPRTPDGREINFYKVIDYILHGKEDIKVIPN
                     PPPDHWALVSGLPAYVAQNGLICNIMNAPAEDFFAFQKEPLDESGWMIKNVLSMPIVN
                     KKEEIVGVATFYNRKDGKPFDEMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIF
                     QDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQAELPDADKYEINKFHF
                     SDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRKITYHNWRHGFN
                     VGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGS
                     SILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQ
                     KIVDQSKTYESEQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSQVALLVAA
                     EFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPM
                     LDGITNNRKEWKALADEYDAKMKVQEEKKQKQQSAKSAAAGNQPGGNPSPGGATTSKS
                     CCIQ"
     exon            595..747
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            748..837
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            838..978
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1053
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1118
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1185
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1233
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1383
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1527
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1593
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1740
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1848
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1958
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2046
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2147
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2255
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2319
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2394
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2478
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..2626
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..5645
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5622..5627
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /note="hexamer: AATAAA"
     polyA_site      5645
                     /gene="PDE6A"
                     /gene_synonym="CGPR-A; PDEA; RP43"
                     /note="major polyA site"
ORIGIN      
        1 agtatgtttt gcagacaaga cccagagaag tccagactgg acttgttgca gactgcaaaa
       61 ctgccattgg aaggcctccg tcccagtcct tctacagagt agccagtggg attcccagcc
      121 atgggcgagg tgacagcaga ggaggtggag aagttcctgg actcgaatat tggctttgcc
      181 aaacagtact acaacctcca ctaccgggcc aagctcatct ccgacctcct tggggccaag
      241 gaggctgccg tggacttcag caactaccac tccccgagca gcatggagga gagcgaaatc
      301 atctttgatc tcctgcggga ctttcaggag aatttacaga cagagaaatg catcttcaat
      361 gtcatgaaga agctgtgctt cctcctgcag gcagaccgca tgagcctgtt catgtaccgg
      421 acccgcaatg gcatcgcaga gctggccacc aggcttttca atgtccacaa ggatgctgtc
      481 ctcgaggact gcctggtgat gcccgaccaa gagatcgtct tccctttgga catgggcatc
      541 gtgggccatg tcgcacactc taagaagatt gctaacgtcc ccaacacaga ggaggatgag
      601 catttctgtg actttgtgga catcctcaca gagtacaaga ccaagaacat cttggcttcc
      661 cccataatga atgggaagga tgtggtggcc ataatcatgg ctgtgaataa agtggatgga
      721 tcccacttca ccaagagaga tgaagagatt cttctcaagt acctcaattt tgcaaatcta
      781 atcatgaagg tgtaccacct gagttacctg cacaactgtg aaactcgacg tggccagata
      841 ctgctgtggt ctgggagcaa agtctttgaa gaacttacgg acatcgaacg acagttccac
      901 aaagccctgt acacagtccg tgctttcctc aactgtgaca gatactctgt gggtctctta
      961 gacatgacca agcagaagga attttttgat gtgtggccgg ttctgatggg tgaagttcca
     1021 ccttactctg gtcccaggac tccggatgga agagaaatta acttttacaa ggtcattgac
     1081 tacatcctgc atggcaaaga ggacatcaaa gtcatcccga atccacctcc tgaccattgg
     1141 gctttagtaa gcggtctccc agcttatgtt gcccagaatg gcctgatttg caacatcatg
     1201 aatgcgcctg cggaggactt ttttgcattt cagaaagaac ctctggatga gtctggatgg
     1261 atgattaaaa atgtgctttc aatgccgatt gtgaacaaga aggaagaaat tgttggagtg
     1321 gccacatttt acaatcgtaa agatgggaag ccctttgatg aaatggatga gacgctcatg
     1381 gagtctttga ctcaatttct gggctggtct gtcttaaatc ctgacaccta tgagtcaatg
     1441 aataaacttg aaaataggaa ggatattttc caggacatag taaaatatca tgtgaagtgt
     1501 gacaatgaag aaattcagaa aatcttgaaa accagagagg tgtatgggaa ggagccatgg
     1561 gagtgtgagg aagaggagct ggctgagatc ctgcaagcgg agctgccaga tgcagataaa
     1621 tacgaaatta ataaatttca cttcagtgac ttacccctaa cagaactgga gctggtaaaa
     1681 tgtggaatac agatgtatta tgagctcaaa gtggtggata aatttcacat tccacaagag
     1741 gccctggtgc ggttcatgta ctccctgagt aagggctacc gcaagatcac ctaccacaac
     1801 tggcggcacg gcttcaacgt ggggcagacc atgttctccc tgctggtgac gggaaagctg
     1861 aagcgctact tcacggacct agaggccttg gccatggtca ctgctgcttt ctgccatgac
     1921 attgaccaca gaggcaccaa taacctctac cagatgaaat cccagaaccc actggccaag
     1981 ctccatgggt cctctatctt ggaaagacac cacttggagt ttggcaaaac actgctcaga
     2041 gacgagagcc tgaatatctt tcaaaacctc aatcgtcgac agcatgagca tgccatccac
     2101 atgatggaca ttgcaatcat tgccacagac ctcgccctgt atttcaagaa gaggacgatg
     2161 ttccaaaaga tcgtggatca gtctaagaca tatgagagtg aacaggagtg gacacagtac
     2221 atgatgctgg agcagacacg gaaggaaatc gttatggcca tgatgatgac cgcctgtgat
     2281 ctctcagcca tcaccaaacc ctgggaggtg cagagccagg tagctctgct ggtggctgct
     2341 gaattctggg aacaaggtga cctggagcgc acggtgctgc aacagaatcc cattcccatg
     2401 atggacagaa acaaagcaga tgaactccct aagcttcaag tcggcttcat tgactttgtt
     2461 tgcaccttcg tctacaagga attctcccgt ttccacgagg agatcacccc aatgttggac
     2521 gggatcacca acaatcgcaa ggagtggaag gcgcttgctg atgagtacga tgccaagatg
     2581 aaggtgcagg aggagaagaa gcagaaacag cagtcggcca agtcagcagc cgcaggaaat
     2641 cagccggggg gaaaccccag cccagggggt gcaactacat ccaagtcctg ctgcatccag
     2701 taacaccact ggggatgtgc tggctggacg gcaccaccct ttcctgggaa gagatgactc
     2761 aagccagtgg aagaccacac accttgagaa gtagaagagt cataggattt gaaagctgtt
     2821 agagaattta gcttccagga ctgttcaatc ttttggcttc cctgggccac attggaagaa
     2881 ttgtcttggg tcacacataa aatacagtaa cactaatgat agctgttgaa cttaaaaaaa
     2941 aaaatcgcca aaaaaaaaaa tttcataatg ttttaagaaa gtttatgaat ttgtgttggg
     3001 ccgcattcaa agccgtcctg ggctgcatgt ggcccacggg ccgtgggttg aacaagcttg
     3061 gcttatacct tatccagtgg cttttaaaca ttcttttagt cttgagaact gcctattgag
     3121 ctaaaaccaa tattctagct ttcatagaca tcagttaaac atttaattac atttaattaa
     3181 tgccagattc actggcttgc ttagaatcat ttttgttctt atactttcag ttaatgtatt
     3241 gtaaaaattt tcaatcattt tcaaaagtag agaattgtac gttgaatccc cacataccca
     3301 tcacccagat tccaatatca agattttgct acatgcattt cgtctgtcct ttttacattt
     3361 tcttttcaat tttttttctt acctgaagtg tttaaaggca aatcctggcc aggcgtggtg
     3421 gctcacacct gtaatcccag cacttcagga ggctgaggtg ggcagactgc atgagcccag
     3481 gagtctgaga ccaacctgag caaaatggca aaaccctgtc tctacaaaaa atacaaaaat
     3541 tagcactcac ctgtagtctc agctacctgg gaggcagagg tgagaggatc tcttgaaccc
     3601 aggaggcgga agttgcagtg agctgagatc atgctactgc actccagctt gggcgaccga
     3661 gcgagactct gactcaaaca aaagcaaaag aaaaggcaaa tcccaaacat atcatttcac
     3721 acctctacta tctcatcttt aaagaaataa gtatgttttc taacataacc acatcactgg
     3781 cccatgcaac caatgcctgc ctaataccca gctgtgatct atgctttgat gggctgcaca
     3841 gaccccaagc ttcagcaaag gacttgggac cccaccagct ttgagtgctg ttgacagacc
     3901 cttagtatca accccatcag gtgttactgc agccttcccc agggtggcct acacccaatg
     3961 actgatccat gccaggtgga gaggcctggc catcttggct gagcatggga taactgtgca
     4021 gggctgttcc agctccacga ctcctcgtgg ggtccactaa ggctgtcgct gggctcacat
     4081 cccaactcga cttctccctc tgcccactcc tatttccttc ccttccccaa gcatcggtcc
     4141 ccagggcact gcttaataag catcctgcag actaagtcca ttttagagtc tgctttctgg
     4201 gacacccaac ttgtgatgcc agcccttaat caaccttcct tcattctcat gaacgttttc
     4261 aatggttggc ttgcctgaat ctgatccaaa caaattcaca tattgaattt agttgtttct
     4321 ttttgttggt ttttagaaaa aagaagatgt actttttatt cttggatttt tcattgattt
     4381 cacagagtat aaattaagtc agattattct caattccaga acttctgttt cttaaattag
     4441 caggcattct gttggtttaa aaagatagat gttggccggg cgcggtggct cacgcctgta
     4501 attccagcac tttgggaggc caaggtgggt ggatcacctg aggtcaggag ttcaagacca
     4561 gcctggccaa catggtgaaa ccccatctct actaaaaata caaaaattag ctgggtgtgg
     4621 tggtgtgcac ctgtaatccc agctactcag gaggctgagg caggagaatc gcttgaacct
     4681 gggaggcgaa ggttgcagtg agctgagatc atgccactgc actccagcct gggcaacaag
     4741 agcaagactc cgtctcaaaa aaaaaaaaaa gaaaaaaaaa agatgtggca gatagaattt
     4801 ctctctttct ctatctttca tgacgaggtc tcactctgtc gcccaggctg gagtgcagtg
     4861 gtatgaccat agctcactgg agccccagct gcccgggctc aagcaatcct ccatctcagc
     4921 ttaccaagca gcggtgacca caggcatgca ccaccatgcc cagctaattt tttaaaacct
     4981 atctttgtag acatggggtc tcattctgtt gcccaggctg gcctcaaact cctgacttca
     5041 agcaatcctc ctgcctcagc ctcccaaagt gttgggatta caggcgtgag ccactgcacc
     5101 tggccaggac ttatttcttt taaaccgaat ctaagtatgt tctggcaagt atgcaatacc
     5161 tagcggaatg cactggcctg atcagcattc ataccatagt aactgatgtt gaaaacagca
     5221 caaataaacc agaagttgaa aatggcaata acatgctaaa aatttgcaag tcaatttgtt
     5281 acacttgtcc aaatcttgat tttttttcct tctctatcgt tcccccccca ccaccagctt
     5341 tatggaggta taattagcaa ataaaaagta tacatattta aggtatacac tgtgatgttt
     5401 tgatatacat tgtgaaatgg ttaccataat taagctaatt aatatatcca tcacctcaca
     5461 tagccatggt taagtttctg aagtctcttt taatctacaa agtactcctt ccttttttcc
     5521 attgtcatca ccttgcagaa acagagtcag ttttcccaca ttctgtattt atctgtttgt
     5581 ttctttgtgg ccgcatgtaa cacattcctc tgtctcattc taataaatgg acattagctc
     5641 tagaa
//
