LOCUS       NM_001104631            8160 bp    mRNA    linear   PRI 23-APR-2024
DEFINITION  Homo sapiens phosphodiesterase 4D (PDE4D), transcript variant 1,
            mRNA.
ACCESSION   NM_001104631
VERSION     NM_001104631.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Nummela,P., Zafar,S., Veikkolainen,E., Ukkola,I., Cinella,V.,
            Ayo,A., Asghar,M.Y., Valimaki,N., Tornquist,K., Karhu,A.,
            Laakkonen,P., Aaltonen,L.A. and Ristimaki,A.
  TITLE     GNAS mutation inhibits growth and induces phosphodiesterase 4D
            expression in colorectal cancer cell lines
  JOURNAL   Int J Cancer 154 (11), 1987-1998 (2024)
   PUBMED   38319157
  REMARK    GeneRIF: GNAS mutation inhibits growth and induces
            phosphodiesterase 4D expression in colorectal cancer cell lines.
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Mydlarova Blascakova,M., Hricova,K., Maslejova,K., Majherova,M.,
            Vaskova,H., Poracova,J., Bernatova,R., Konecna,M. and Sedlak,V.
  TITLE     Association of the PDE4D gene variant with selected markers in
            individuals with ischaemic heart disease: a pilot study
  JOURNAL   Cent Eur J Public Health 31 (Suppl 1), S75-S81 (2023)
   PUBMED   38272484
  REMARK    GeneRIF: Association of the PDE4D gene variant with selected
            markers in individuals with ischaemic heart disease: a pilot study.
REFERENCE   3  (bases 1 to 8160)
  AUTHORS   Rosand,J., Bayley,N., Rost,N. and de Bakker,P.I.
  TITLE     Many hypotheses but no replication for the association between
            PDE4D and stroke
  JOURNAL   Nat Genet 38 (10), 1091-2 (2006)
   PUBMED   17006457
REFERENCE   4  (bases 1 to 8160)
  AUTHORS   Gretarsdottir,S., Thorleifsson,G., Reynisdottir,S.T., Manolescu,A.,
            Jonsdottir,S., Jonsdottir,T., Gudmundsdottir,T., Bjarnadottir,S.M.,
            Einarsson,O.B., Gudjonsdottir,H.M., Hawkins,M., Gudmundsson,G.,
            Gudmundsdottir,H., Andrason,H., Gudmundsdottir,A.S.,
            Sigurdardottir,M., Chou,T.T., Nahmias,J., Goss,S.,
            Sveinbjornsdottir,S., Valdimarsson,E.M., Jakobsson,F.,
            Agnarsson,U., Gudnason,V., Thorgeirsson,G., Fingerle,J., Gurney,M.,
            Gudbjartsson,D., Frigge,M.L., Kong,A., Stefansson,K. and
            Gulcher,J.R.
  TITLE     The gene encoding phosphodiesterase 4D confers risk of ischemic
            stroke
  JOURNAL   Nat Genet 35 (2), 131-138 (2003)
   PUBMED   14517540
  REMARK    GeneRIF: fine mapping of locus and testing for association with
            stroke
            Erratum:[Nat Genet. 2005 May;37(5):555]
REFERENCE   5  (bases 1 to 8160)
  AUTHORS   Dominiczak,A.F. and McBride,M.W.
  TITLE     Genetics of common polygenic stroke
  JOURNAL   Nat Genet 35 (2), 116-117 (2003)
   PUBMED   14517535
REFERENCE   6  (bases 1 to 8160)
  AUTHORS   Le Jeune,I.R., Shepherd,M., Van Heeke,G., Houslay,M.D. and
            Hall,I.P.
  TITLE     Cyclic AMP-dependent transcriptional up-regulation of
            phosphodiesterase 4D5 in human airway smooth muscle cells.
            Identification and characterization of a novel PDE4D5 promoter
  JOURNAL   J Biol Chem 277 (39), 35980-35989 (2002)
   PUBMED   12121997
  REMARK    GeneRIF: PDE4D5 is upregulated in tracheal smooth muscle cells
            because of a CRE-containing, isoform-specific promoter
REFERENCE   7  (bases 1 to 8160)
  AUTHORS   Nemoz,G., Zhang,R., Sette,C. and Conti,M.
  TITLE     Identification of cyclic AMP-phosphodiesterase variants from the
            PDE4D gene expressed in human peripheral mononuclear cells
  JOURNAL   FEBS Lett 384 (1), 97-102 (1996)
   PUBMED   8797812
REFERENCE   8  (bases 1 to 8160)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
REFERENCE   9  (bases 1 to 8160)
  AUTHORS   Milatovich,A., Bolger,G., Michaeli,T. and Francke,U.
  TITLE     Chromosome localizations of genes for five cAMP-specific
            phosphodiesterases in man and mouse
  JOURNAL   Somat Cell Mol Genet 20 (2), 75-86 (1994)
   PUBMED   8009369
REFERENCE   10 (bases 1 to 8160)
  AUTHORS   Swinnen,J.V., Joseph,D.R. and Conti,M.
  TITLE     The mRNA encoding a high-affinity cAMP phosphodiesterase is
            regulated by hormones and cAMP
  JOURNAL   Proc Natl Acad Sci U S A 86 (21), 8197-8201 (1989)
   PUBMED   2554303
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027322.4, L20969.1,
            AC092343.3 and BF197530.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001104631.1.
            
            Summary: This gene encodes one of four mammalian counterparts to
            the fruit fly 'dunce' gene. The encoded protein has
            3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP,
            which acts as a signal transduction molecule in multiple cell
            types. This gene uses different promoters to generate multiple
            alternatively spliced transcript variants that encode functional
            proteins.[provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (PDE4D4, also known as isoform 1).
            
            Sequence Note: The sequence L20969.1 is from a chimeric clone. Only
            the region representing PDE4D was propagated into this RefSeq
            record. This RefSeq record was created from transcript and genomic
            sequence data to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005449.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340635.11/ ENSP00000345502.6
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                AC027322.4         148810-148856       c
            48-2719             L20969.1           366-3037
            2720-7706           AC092343.3         126856-131842
            7707-8160           BF197530.1         13-466              c
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q11.2-q12.1"
     gene            1..8160
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /note="phosphodiesterase 4D"
                     /db_xref="GeneID:5144"
                     /db_xref="HGNC:HGNC:8783"
                     /db_xref="MIM:600129"
     exon            1..559
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2534
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /EC_number="3.1.4.53"
                     /note="isoform PDE4D4 is encoded by transcript variant 1;
                     phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3
                     dunce homolog, Drosophila); cAMP-specific 3',5'-cyclic
                     phosphodiesterase 4D; cAMP-specific phosphodiesterase
                     PDE4D6; testicular tissue protein Li 136; 3',5'-cyclic-AMP
                     phosphodiesterase 4D"
                     /codon_start=1
                     /product="3',5'-cyclic-AMP phosphodiesterase 4D isoform
                     PDE4D4"
                     /protein_id="NP_001098101.1"
                     /db_xref="CCDS:CCDS47213.1"
                     /db_xref="GeneID:5144"
                     /db_xref="HGNC:HGNC:8783"
                     /db_xref="MIM:600129"
                     /translation="MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLR
                     QPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATG
                     RVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTS
                     AGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHG
                     DDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKL
                     ASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFIS
                     NTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQED
                     VLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMT
                     LEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPG
                     VSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMV
                     IDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNP
                     TKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPL
                     WETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTL
                     EEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPE
                     ACVIDDRSPDT"
     exon            560..751
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            752..788
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            789..862
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            863..912
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1025
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1119
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1292
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1391
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1556
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1656
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1811
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1934
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2117
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..8160
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7101..7106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /note="hexamer: AATAAA"
     polyA_site      7127
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /note="major polyA site"
     regulatory      8129..8134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
                     /note="hexamer: ATTAAA"
     polyA_site      8160
                     /gene="PDE4D"
                     /gene_synonym="ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2;
                     STRK1"
ORIGIN      
        1 gcagaagcgg cggcggcggc agcagcagca gcagcatcag gaaggcgctc gggccagcgc
       61 ggtgaacccg ggctgggcag caggtcgcgg agccgcgagc caggatggag gcagagggca
      121 gcagcgcgcc ggcccgggcg ggcagcggag agggcagcga cagcgccggc ggggccacgc
      181 tcaaagcccc caagcatctc tggaggcacg agcagcacca ccagtacccg ctccggcagc
      241 cccagttccg cctcctgcat ccccatcacc acctgccccc gccgccgcca ccctcgcccc
      301 agccccagcc ccagtgtccg ctacagccgc cgccgccgcc ccccctgccg ccgcccccgc
      361 cgccgcccgg ggctgcccgc ggccgctacg cctcgagcgg ggccaccggc cgcgtccggc
      421 atcgcggcta ctcggacacc gagcgctacc tgtactgtcg cgccatggac cgcacctcct
      481 acgcggtgga gaccggccac cggcccggcc tgaagaaatc caggatgtcc tggccctcct
      541 cgttccaggg actcaggcgt tttgatgtgg acaatggcac atctgcggga cggagtccct
      601 tggatcccat gaccagccca ggatccgggc taattctcca agcaaatttt gtccacagtc
      661 aacgacggga gtccttcctg tatcgatccg acagcgatta tgacctctct ccaaagtcta
      721 tgtcccggaa ctcctccatt gccagtgata tacacggaga tgacttgatt gtgactccat
      781 ttgctcaggt cttggccagt ctgcgaactg tacgaaacaa ctttgctgca ttaactaatt
      841 tgcaagatcg agcacctagc aaaagatcac ccatgtgcaa ccaaccatcc atcaacaaag
      901 ccaccataac agaggaggcc taccagaaac tggccagcga gaccctggag gagctggact
      961 ggtgtctgga ccagctagag accctacaga ccaggcactc cgtcagtgag atggcctcca
     1021 acaagtttaa aaggatgctt aatcgggagc tcacccatct ctctgaaatg agtcggtctg
     1081 gaaatcaagt gtcagagttt atatcaaaca cattcttaga taagcaacat gaagtggaaa
     1141 ttccttctcc aactcagaag gaaaaggaga aaaagaaaag accaatgtct cagatcagtg
     1201 gagtcaagaa attgatgcac agctctagtc tgactaattc aagtatccca aggtttggag
     1261 ttaaaactga acaagaagat gtccttgcca aggaactaga agatgtgaac aaatggggtc
     1321 ttcatgtttt cagaatagca gagttgtctg gtaaccggcc cttgactgtt atcatgcaca
     1381 ccatttttca ggaacgggat ttattaaaaa catttaaaat tccagtagat actttaatta
     1441 catatcttat gactctcgaa gaccattacc atgctgatgt ggcctatcac aacaatatcc
     1501 atgctgcaga tgttgtccag tctactcatg tgctattatc tacacctgct ttggaggctg
     1561 tgtttacaga tttggagatt cttgcagcaa tttttgccag tgcaatacat gatgtagatc
     1621 atcctggtgt gtccaatcaa tttctgatca atacaaactc tgaacttgcc ttgatgtaca
     1681 atgattcctc agtcttagag aaccatcatt tggctgtggg ctttaaattg cttcaggaag
     1741 aaaactgtga cattttccag aatttgacca aaaaacaaag acaatcttta aggaaaatgg
     1801 tcattgacat cgtacttgca acagatatgt caaaacacat gaatctactg gctgatttga
     1861 agactatggt tgaaactaag aaagtgacaa gctctggagt tcttcttctt gataattatt
     1921 ccgataggat tcaggttctt cagaatatgg tgcactgtgc agatctgagc aacccaacaa
     1981 agcctctcca gctgtaccgc cagtggacgg accggataat ggaggagttc ttccgccaag
     2041 gagaccgaga gagggaacgt ggcatggaga taagccccat gtgtgacaag cacaatgctt
     2101 ccgtggaaaa atcacaggtg ggcttcatag actatattgt tcatcccctc tgggagacat
     2161 gggcagacct cgtccaccct gacgcccagg atattttgga cactttggag gacaatcgtg
     2221 aatggtacca gagcacaatc cctcagagcc cctctcctgc acctgatgac ccagaggagg
     2281 gccggcaggg tcaaactgag aaattccagt ttgaactaac tttagaggaa gatggtgagt
     2341 cagacacgga aaaggacagt ggcagtcaag tggaagaaga cactagctgc agtgactcca
     2401 agactctttg tactcaagac tcagagtcta ctgaaattcc ccttgatgaa caggttgaag
     2461 aggaggcagt aggggaagaa gaggaaagcc agcctgaagc ctgtgtcata gatgatcgtt
     2521 ctcctgacac gtaacagtgc aaaaactttc atgccttttt tttttttaag tagaaaaatt
     2581 gtttccaaag tgcatgtcac atgccacaac cacggtcaca cctcactgtc atctgccagg
     2641 acgtttgttg aacaaaactg accttgacta ctcagtccag cgctcaggaa tatcgtaacc
     2701 agttttttca cctccatgtc atccgagcaa ggtggacatc ttcacgaaca gcgtttttaa
     2761 caagatttca gcttggtaga gctgacaaag cagataaaat ctactccaaa ttattttcaa
     2821 gagagtgtga ctcatcaggc agcccaaaag tttattggac ttggggtttc tattcctttt
     2881 tatttgtttg caatattttc agaagaaagg cattgcacag agtgaactta atggacgaag
     2941 caacaaatat gtcaagaaca ggacatagca cgaatctgtt accagtagga ggaggatgag
     3001 ccacagaaat tgcataattt tctaatttca agtcttcctg atacatgact gaatagtgtg
     3061 gttcagtgag ctgcactgac ctctacattt tgtatgatat gtaaaacaga ttttttgtag
     3121 agcttacttt tattattaaa tgtattgagg tattatattt aaaaaaaact atgttcagaa
     3181 cttcatctgc cactggttat ttttttctaa ggagtaactt gcaagttttc agtacaaatc
     3241 tgtgctacac tggataaaaa tctaatttat gaattttact tgcaccttat agttcatagc
     3301 aattaactga tttgtagtga ttcattgttt gttttatata ccaatgactt ccatatttta
     3361 aaagagaaaa acaactttat gttgcaggaa accctttttg taagtcttta ttatttactt
     3421 tgcattttgt ttcactcttt ccagataagc agagttgctc ttcaccagtg tttttcttca
     3481 tgtgcaaagt gactatttgt tctataatac ttttatgtgt gttatatcaa atgtgtctta
     3541 agcttcatgc aaactcagtc atcagttcgt gttgtctgaa gcaagtggga gatatataaa
     3601 tacccagtag ctaaaatggt cagtcttttt tagatgtttt cctacttagt atctcctaat
     3661 aacgttttgc tgtgtcacta gatgttcatt tcacaagtgc atgtctttct aataatccac
     3721 acatttcatg ctctaataat ccacacattt catgctcatt tttattgttt ttacagccag
     3781 ttatagtaag aaaaaggttt ttccccttgt gctgctttat aatttagcgt gtgtctgaac
     3841 cttatccatg tttgctagat gaggtcttgt caaatatatc actaccattg tcaccggtga
     3901 aaagaaacag gtagttaagt tagggttaac attcatttca accacgaggt tgtatatcat
     3961 gactagcttt tactcttggt ttacagagaa aagttaaaca gccaactagg cagtttttaa
     4021 gaatattaac aatatattaa caaacaccaa tacaactaat cctatttggt tttaatgatt
     4081 tcaccatggg attaagaact atatcaggaa catccctgag aaacggtttt aagtgtagca
     4141 actactcttc cttaatggac agccacataa cgtgtaggaa gtcctttatc acttatcctc
     4201 gatccataag catatcttgc agaggggaac tacttcttta aacacatgga gggaaagaag
     4261 atgatgccac tggcaccaga gggttagtac tgtgatgcat cctaaaatat ttattatatt
     4321 ggtaaaaatt ctggttaaat aaaaaattag agatcactct tggctgattt cagcaccagg
     4381 aactgtatta cagttttaga gattaattcc tagtgtttac ctgattatag cagttggcat
     4441 catggggcat ttaattctga ctttatcccc acgtcagcct taataaagtc ttctttacct
     4501 tctctatgaa gactttaaag cccaaataat catttttcac attgatattc aagaattgag
     4561 atagatagaa gccaaagtgg gtatctgaca agtggaaaat caaacgttta agaagaatta
     4621 caactctgaa aagcatttat atgtggaact tctcaaggag cctcctgggg actggaaagt
     4681 aagtcatcag ccaggcaaat gactcatgct gaagagagtc cccatttcag tcccctgaga
     4741 tctagctgat gcttagatcc tttgaaataa aaattatgtc tttataactc tgatctttta
     4801 cataaagcag aagaggaatc aactagttaa ttgcaaggtt tctactctgt ttcctctgta
     4861 aagatcagat ggtaatcttt caaataagaa aaaaataaag acgtatgttt gaccaagtag
     4921 tttcacaaga atatttggga acttgtttct tttaatttta tttgtccctg agtgaagtct
     4981 agaaagaaag gtaaagagtc tagagtttat tcctctttcc aaaacattct cattcctctc
     5041 ctccctacac ttagtatttc ccccacagag tgcctagaat cttaataatg aataaaataa
     5101 aaagcagcaa tatgtcatta acaaatccag acctgaaagg gtaaagggtt tataactgca
     5161 ctaataaaga gaggctcttt ttttttcttc cagtttgttg gtttttaatg gtaccgtgtt
     5221 gtaaagatac ccactaatgg acaatcaaat tgcagaaaag gctcaatatc caagagacag
     5281 ggactaatgc actgtacaat ctgcttatcc ttgcccttct ctcttgccaa agtgtgcttc
     5341 agaaatatat actgctttaa aaaagaataa aagaatatcc ttttacaagt ggctttacat
     5401 ttcctaaaat gccataagaa aatgcaatat ctgggtactg tatggggaaa aaaatgtcca
     5461 agtttgtgta aaaccagtgc atttcagctt gcaagttact gaacacaata atgctgtttt
     5521 aattttgttt tatatcagtt aaaattcaca ataatgtaga tagaacaaat tacagacaag
     5581 gaaagaaaaa acttgaatga aatggatttt acagaaagct ttatgataat ttttgaatgc
     5641 attatttatt ttttgtgcca tgcatttttt ttctcaccaa atgaccttac ctgtaataca
     5701 gtcttgtttg tctgtttaca accatgtatt tattgcaatg tacatactgt aatgttaatt
     5761 gtaaattatc tgttcttatt aaaacatcat cccatgatgg gatggtgttg atatatttgg
     5821 aaactcttgg tgagagaatg aatggtgtgt atacatactc tgtacatttt tcttttctcc
     5881 tgtaatatag tcttgtcacc ttagagcttg tttatggaag attcaagaaa actataaaat
     5941 acttaaagat atataaattt aaaaaaacat agctgcaggt ctttggtccc agggctgtgc
     6001 cttaacttta accaatattt tcttctgttt tgctgcattt gaaaggtaac agtggagcta
     6061 gggctgggca ttttacatcc aggcttttaa ttgattagaa ttctgccaat aggtggattt
     6121 tacaaaacca cagacaacct ctgaaagatt ctgagaccct tttgagacag aagctcttaa
     6181 gtacttcttg ccagggagca gcactgcatg tgtgatggtt gtttgccatc tgttgatcag
     6241 gaactacttc agctacttgc atttgattat ttcctttttt ttttttttta actcggaaac
     6301 acaactgggg aaatatattc tttcccagtg attataaaca atctttttct tttttttaag
     6361 tccttttggc ttctagagct cataggaaaa tggacttgat ttgaaattgg agccagagtt
     6421 tactcgtgtt ggttatctat tcatcagctt cctgacatgt taagagaata cattaaagag
     6481 aaaatactgt tttttaatcc taaaattttt cttccactaa gataaaccaa atgtccttac
     6541 atatatgtaa acccatctat ttaaacgcaa aggtgggttg atgtcagttt acatagcaga
     6601 aagcattcac tatcctctaa gatttgtttc tgcaaaactt tcattgcttt agaattttaa
     6661 aatttcacct tgtacaatgg ccagccccta aagcaggaaa catttataat ggattatatg
     6721 gaaacatcct cccagtactt gcccagccct tgaatcatgt ggcttttcag tgaaaggaaa
     6781 gattcttttt ctaggaaaaa tgagcctatt ttattttatt ttattttatt ttttgacaca
     6841 aactgtagat tttagcagcc ctggcccaaa ggaatttgat tacttttgtt ttaaacagta
     6901 caaaggggac actataatta caaaaacatc cttaactgat ttgagttgtt tttatttctt
     6961 tggatatatt ttcagagtgg taaattgtgt gtgagaatta caaatgatta ttcttttagt
     7021 ggtttcttag cctctcttac agcccacggg gatagtactg tacatcaata ccttcatatg
     7081 aaatttttat atgcaatgaa aataaaagca tgggttgatt ctgcctattt atgactcaat
     7141 cttttacaaa taaaagatta ttcattttaa attatagttc aatcagcatg tctcttagga
     7201 tactgaacgt ggttgaaatg aaaggatagt gacatcataa gttagtactg atattcataa
     7261 ccaaataaag ccaacttgag taattttgct acattaaaaa ttaccaaaat tacttagatg
     7321 gcctataaga ttaagcatgg tgttttctaa gcaagctttg aaaggggcct tccatactta
     7381 cttaattgaa tattctggga tattgaaaat tattcagata cttgacaatt atttttggtt
     7441 acctactccg caaactacaa agttttaagg actcaacaat aagttaatga gacacagtgt
     7501 ttgctttcat ggagcttaca gtctggaggg gacaaaggct taaacaatac tcatataatt
     7561 atatatgtga tcagtacaat gaaggagctc agtggggtaa ataagcagga acctgaactt
     7621 gatctgttcc ggagggccac agaaggcttc cttgaggcct tgagaaagtg atttgcatct
     7681 gagttctgaa ggattgtaag aggtaactag ggaaaaagtt gacaggaaga ggaaggggat
     7741 ccagacaaga aacatttgca aagatcttga ggcataaatg agcttgagac atctggagaa
     7801 actgaggaaa agtgagagag taggcagggc ctggagccgc agagccattg ctaaccatcc
     7861 tgtgtgagat atcccccatt ctgtagcttt attctcataa ccctgctcaa ttttctttat
     7921 aacacttctc acagatttat atacgtgttt gtttttgtta tctgtctctc ccaccagacc
     7981 acagctccat gagagcaagg tctttgctta ccaatatatc actagcactt aaaactatgc
     8041 ctggtacaca gtaggttctt aatatgtgtt gaatatagcc atcaaattga tattggatat
     8101 aattcaatct gataagatat tttgagatat taaagagttt ttaacttgat accataaaaa
//
