LOCUS       NM_001363871            5037 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 6,
            mRNA.
ACCESSION   NM_001363871 XM_017004302
VERSION     NM_001363871.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Tam,C.H.T., Lim,C.K.P., Luk,A.O.Y., Shi,M., Man Cheung,H.,
            Ng,A.C.W., Lee,H.M., Lau,E.S.H., Fan,B., Jiang,G., Kong,A.P.S.,
            Ozaki,R., Chow,E.Y.K., Lee,K.F., Siu,S.C., Hui,G., Tsang,C.C.,
            Lau,K.P., Leung,J.Y.Y., Cheung,E.Y.N., Tsang,M.W., Kam,G.,
            Lau,I.T., Li,J.K.Y., Yeung,V.T.F., Lau,E., Lo,S., Fung,S.,
            Cheng,Y.L., Chow,C.C., Fan,X., Chan,T.F., Yip,K.Y.L., Lok,S.,
            Yu,W., Tsui,S.K.W., Lan,H.Y., Szeto,C.C., Tang,N.L.S.,
            Tomlinson,B., Huang,Y., Jenkins,A.J., Keech,A., So,W.Y.,
            Chan,J.C.N. and Ma,R.C.W.
  CONSRTM   Hong Kong Diabetes Biobank Study Group, FIELD Study Investigators;
            TRansomics ANalysis of Complications and ENdpoints in Diabetes
            (TRANSCEND) Consortium
  TITLE     Identification of a Common Variant for Coronary Heart Disease at
            PDE1A Contributes to Individualized Treatment Goals and Risk
            Stratification of Cardiovascular Complications in Chinese Patients
            With Type 2 Diabetes
  JOURNAL   Diabetes Care 46 (6), 1271-1281 (2023)
   PUBMED   37125963
  REMARK    GeneRIF: Identification of a Common Variant for Coronary Heart
            Disease at PDE1A Contributes to Individualized Treatment Goals and
            Risk Stratification of Cardiovascular Complications in Chinese
            Patients With Type 2 Diabetes.
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 5037)
  AUTHORS   Amin,S.A., Bhargava,S., Adhikari,N., Gayen,S. and Jha,T.
  TITLE     Exploring pyrazolo[3,4-d]pyrimidine phosphodiesterase 1 (PDE1)
            inhibitors: a predictive approach combining comparative validated
            multiple molecular modelling techniques
  JOURNAL   J Biomol Struct Dyn 36 (3), 590-608 (2018)
   PUBMED   28132591
  REMARK    GeneRIF: the p-substituted benzyl side chain at N2-position helps
            to enhance the PDE1 inhibitory profile. Depending on these
            observations, some new molecules are predicted that may possess
            better PDE1 inhibition
REFERENCE   4  (bases 1 to 5037)
  AUTHORS   Yang,Z., Zhou,T., Sun,B., Wang,Q., Dong,X., Hu,X., Zhong,J.,
            Song,B. and Li,L.
  TITLE     PDE1A polymorphism contributes to the susceptibility of
            nephrolithiasis
  JOURNAL   BMC Genomics 18 (1), 982 (2017)
   PUBMED   29262781
  REMARK    GeneRIF: evidence that the rs182089527 mutation in PDE1A is
            involved in the development of nephrolithiasis and kidney cysts.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5037)
  AUTHORS   Bautista Nino,P.K., Durik,M., Danser,A.H., de Vries,R.,
            Musterd-Bhaggoe,U.M., Meima,M.E., Kavousi,M., Ghanbari,M.,
            Hoeijmakers,J.H., O'Donnell,C.J., Franceschini,N., Janssen,G.M., De
            Mey,J.G., Liu,Y., Shanahan,C.M., Franco,O.H., Dehghan,A. and
            Roks,A.J.
  TITLE     Phosphodiesterase 1 regulation is a key mechanism in vascular aging
  JOURNAL   Clin Sci (Lond) 129 (12), 1061-1075 (2015)
   PUBMED   26464516
  REMARK    GeneRIF: Report significant associations of PDE1A single nucleotide
            polymorphisms with diastolic blood pressure and carotid
            intima-media thickness.
REFERENCE   6  (bases 1 to 5037)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   7  (bases 1 to 5037)
  AUTHORS   Zauli,G., Milani,D., Mirandola,P., Mazzoni,M., Secchiero,P.,
            Miscia,S. and Capitani,S.
  TITLE     HIV-1 Tat protein down-regulates CREB transcription factor
            expression in PC12 neuronal cells through a phosphatidylinositol
            3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway
  JOURNAL   FASEB J 15 (2), 483-491 (2001)
   PUBMED   11156964
REFERENCE   8  (bases 1 to 5037)
  AUTHORS   Michibata,H., Yanaka,N., Kanoh,Y., Okumura,K. and Omori,K.
  TITLE     Human Ca2+/calmodulin-dependent phosphodiesterase PDE1A: novel
            splice variants, their specific expression, genomic organization,
            and chromosomal localization
  JOURNAL   Biochim Biophys Acta 1517 (2), 278-287 (2001)
   PUBMED   11342109
REFERENCE   9  (bases 1 to 5037)
  AUTHORS   Snyder,P.B., Florio,V.A., Ferguson,K. and Loughney,K.
  TITLE     Isolation, expression and analysis of splice variants of a human
            Ca2+/calmodulin-stimulated phosphodiesterase (PDE1A)
  JOURNAL   Cell Signal 11 (7), 535-544 (1999)
   PUBMED   10405764
REFERENCE   10 (bases 1 to 5037)
  AUTHORS   Loughney,K., Martins,T.J., Harris,E.A., Sadhu,K., Hicks,J.B.,
            Sonnenburg,W.K., Beavo,J.A. and Ferguson,K.
  TITLE     Isolation and characterization of cDNAs corresponding to two human
            calcium, calmodulin-regulated, 3',5'-cyclic nucleotide
            phosphodiesterases
  JOURNAL   J Biol Chem 271 (2), 796-806 (1996)
   PUBMED   8557689
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010888.12, AC012500.7 and AC011291.8.
            
            On Jul 2, 2021 this sequence version replaced NM_001363871.3.
            
            Summary: Cyclic nucleotide phosphodiesterases (PDEs) play a role in
            signal transduction by regulating intracellular cyclic nucleotide
            concentrations through hydrolysis of cAMP and/or cGMP to their
            respective nucleoside 5-prime monophosphates. Members of the PDE1
            family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM
            114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin
            in the presence of Ca(2+) (Michibata et al., 2001 [PubMed
            11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by
            OMIM, Oct 2009].
            
            Transcript Variant: This variant (6) differs in the 5' UTR and uses
            an alternate start codon, compared to variant 1. The encoded
            isoform (6) is shorter and has distinct N- terminus, compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2276366.1,
                                           SRR14038194.302722.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409365.6/ ENSP00000386767.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-459               AC010888.12        45433-45891         c
            460-573             AC012500.7         719-832             c
            574-756             AC011291.8         124742-124924       c
            757-823             AC011291.8         119064-119130       c
            824-940             AC011291.8         115647-115763       c
            941-1081            AC011291.8         114638-114778       c
            1082-1182           AC011291.8         108496-108596       c
            1183-1308           AC011291.8         90572-90697         c
            1309-1410           AC011291.8         86320-86421         c
            1411-1531           AC011291.8         86071-86191         c
            1532-1613           AC011291.8         73611-73692         c
            1614-1734           AC011291.8         71100-71220         c
            1735-1922           AC011291.8         70524-70711         c
            1923-2039           AC011291.8         31668-31784         c
            2040-5037           AC011291.8         24673-27670         c
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q32.1"
     gene            1..5037
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /note="phosphodiesterase 1A"
                     /db_xref="GeneID:5136"
                     /db_xref="HGNC:HGNC:8774"
                     /db_xref="MIM:171890"
     exon            1..459
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             407..1996
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /EC_number="3.1.4.17"
                     /note="isoform 6 is encoded by transcript variant 6;
                     calcium/calmodulin-stimulated cyclic nucleotide
                     phosphodiesterase; calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A; 61 kDa
                     Cam-PDE; phosphodiesterase 1A, calmodulin-dependent; dual
                     specificity calcium/calmodulin-dependent 3',5'-cyclic
                     nucleotide phosphodiesterase 1A"
                     /codon_start=1
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A isoform 6"
                     /protein_id="NP_001350800.1"
                     /db_xref="CCDS:CCDS86900.1"
                     /db_xref="GeneID:5136"
                     /db_xref="HGNC:HGNC:8774"
                     /db_xref="MIM:171890"
                     /translation="MDDHVTIRKKHLQRPIFRLRCLVKQLERGDVNVVDLKKNIEYAA
                     SVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKF
                     RSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSL
                     KFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYI
                     MLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHH
                     VSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPE
                     GIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDR
                     KSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGL
                     HIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQGES
                     DLHKNSEDLVNAEEKHDETHS"
     exon            460..573
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            574..756
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            757..823
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            824..940
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1081
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1182
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1308
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1410
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1531
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1613
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1734
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1922
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2039
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..5037
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4573..4578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /note="hexamer: AATAAA"
     polyA_site      4595
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /note="major polyA site"
     regulatory      4732..4737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /note="hexamer: AATAAA"
     polyA_site      4751
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
     regulatory      5014..5019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
                     /note="hexamer: AATAAA"
     polyA_site      5037
                     /gene="PDE1A"
                     /gene_synonym="CAM-PDE 1A; CAM-PDE-1A; HCAM-1; HCAM1;
                     HSPDE1A"
ORIGIN      
        1 ctttcggagc ttgaccagca tgtggaagga gagctctcat ggctgcaata acttagggag
       61 ttcctacctg gatgacactg gggtaggaag ttttctgttt gttttgttct gtttcggagg
      121 gtcccgtgca cttctcttgc ctggatcaac gtgactcttg gtggtttttg cacaccctgc
      181 tctgtggggg agtgccccaa cgtgcctgtc ttatgtggcc tctatggaca cactgctcag
      241 ctggcagaga acagggaagt tttctcccag cacccaaaaa gagaaagagg aaactacttt
      301 ttctttctgg gctccttgca gcacaataga tcaggataag cttccacatt tcctctctgg
      361 agtttctgga gtggtttcca ggaagaagtt aaaccttcac ctttaaatgg atgaccatgt
      421 cacaatcagg aagaaacatc tccaaagacc catctttaga ctaagatgct tggtgaagca
      481 gctggaaaga ggtgatgtta acgtcgtcga cttaaagaag aatattgaat atgcggcatc
      541 tgtgctggaa gcagtttata tcgatgaaac aagaagactt ctggatactg aagatgagct
      601 cagtgacatt cagactgact cagtcccatc tgaagtccgg gactggttgg cttctacctt
      661 tacacggaaa atggggatga caaaaaagaa acctgaggaa aaaccaaaat ttcggagcat
      721 tgtgcatgct gttcaagctg gaatttttgt ggaaagaatg taccgaaaaa catatcatat
      781 ggttggtttg gcatatccag cagctgtcat cgtaacatta aaggatgttg ataaatggtc
      841 tttcgatgta tttgccctaa atgaagcaag tggagagcat agtctgaagt ttatgattta
      901 tgaactgttt accagatatg atcttatcaa ccgtttcaag attcctgttt cttgcctaat
      961 cacctttgca gaagctttag aagttggtta cagcaagtac aaaaatccat atcacaattt
     1021 gattcatgca gctgatgtca ctcaaactgt gcattacata atgcttcata caggtatcat
     1081 gcactggctc actgaactgg aaattttagc aatggtcttt gctgctgcca ttcatgatta
     1141 tgagcataca gggacaacaa acaactttca cattcagaca aggtcagatg ttgccatttt
     1201 gtataatgat cgctctgtcc ttgagaatca ccacgtgagt gcagcttatc gacttatgca
     1261 agaagaagaa atgaatatct tgataaattt atccaaagat gactggaggg atcttcggaa
     1321 cctagtgatt gaaatggttt tatctacaga catgtcaggt cacttccagc aaattaaaaa
     1381 tataagaaac agtttgcagc agcctgaagg gattgacaga gccaaaacca tgtccctgat
     1441 tctccacgca gcagacatca gccacccagc caaatcctgg aagctgcatt atcggtggac
     1501 catggcccta atggaggagt ttttcctgca gggagataaa gaagctgaat tagggcttcc
     1561 attttcccca ctttgtgatc ggaagtcaac catggtggcc cagtcacaaa taggtttcat
     1621 cgatttcata gtagagccaa cattttctct tctgacagac tcaacagaga aaattgttat
     1681 tcctcttata gaggaagcct caaaagccga aacttcttcc tatgtggcaa gcagctcaac
     1741 caccattgtg gggttacaca ttgctgatgc actaagacga tcaaatacaa aaggctccat
     1801 gagtgatggg tcctattccc cagactactc ccttgcagca gtggacctga agagtttcaa
     1861 gaacaacctg gtggacatca ttcagcagaa caaagagagg tggaaagagt tagctgcaca
     1921 aggtgaatct gatcttcata agaactcaga agacttagta aatgctgaag aaaaacatga
     1981 tgagacacat tcataggccc gaaacacctt aaagacttct gtcattttaa acatgagagg
     2041 acaatgaaat cagcatgaaa acatcctaaa ttctcaactt tccacaaagc tatggctctt
     2101 ctttcaacat agaattggat tgggccattt taattgactc ctatacaagg aattaagaag
     2161 aacataaatt ttgagctagt aactctggcc aaataaatac actcaagttt ttatcagagt
     2221 ttttggccag tgcttctgcc atttttttcc ctccacaatt tggccttctt caatcaagcc
     2281 agataaattt ttgagacaaa agtcagacag tttttaattt ttcttgcttt gaaccctgtc
     2341 ataatgactg tgcaatacat gtgcagaaga tgaggtattt taaaatttac ttccttgcac
     2401 tgtcttacac agagtgctat aactataaat ttttcaaggt cttaaataaa aggaagcaaa
     2461 aacaaaatta ttgaaaaatt ttttttgttg tgctggggaa tatactattt agattgtcct
     2521 tcttatttta aatgcatggg aacagaatga caggtgggat gctgaggagc tggttgaagc
     2581 atcagagcaa tgctacagtc caacaatgga gcattagatc cccagctagg tgacctgatg
     2641 aacacctttg taaactctca tactgggaaa tggcagtgaa acctttttct gaacccgtgg
     2701 accagcctgc gtccgtcccc atggtttcaa agcacgaatg cattacctcc atggttgtgg
     2761 ttacaagcaa gtcacatgct atctacagtt catgataaca agaccgtcaa ccattgctgg
     2821 agacagacac tagaatggaa aatgaaattt gccagcggga tgtttatccc atcagaatgt
     2881 aacaaaatat attaccagcc aagagataag agacaagaat gtgtgtgtgt gtgtgtgtgt
     2941 gtgtgtgtgt gtgttcaaaa atgtctatga aaatgaaaac ccacactttt ctgtacagat
     3001 aacattattt gatgtgactt ttatatttta ctacacagaa acaaactgca gccctcagag
     3061 gccctgcttc cttgtcatgt tttgcctgag catgtgcaaa gtctttcctt tgcttaaagc
     3121 tgaaaaacta cctttatgca ttgttagctg acaagaaaga tcaagcacaa ttagatgttg
     3181 taacttttca ctgtacaagc attgcaatga ttgtgagctt cagtgaattt atctttgtga
     3241 aaggtaaaaa ttggctggaa agaatcagga gtagccataa attaaatcat gcaaaggagg
     3301 atccatgcat cctaacaagt tttctgctgc atctgcttcc ttcctgggct ccacaatgtt
     3361 aagcaatatt ttagaaaagg gtacaattgg aagcttttaa gcctgagtgg tgagatttta
     3421 taactcacaa cttagatctc ttttaatgtg taagccatat tctatatgca tatttattat
     3481 gcatcattgt aatttaagat atatttggat gtaggctgta taaatgcttt taattgttct
     3541 gtgaatagat ccttagcttt agtttcacct gaatcttgtg caaaacacag ctaaataatt
     3601 gaattgtatt atagacatta tgaggtaatt gatcttttaa atgtaaggag taagattgat
     3661 ctataataat gtgctgggga aaacatttat agatatgtac atcatggtta ctttttgtcc
     3721 tttcaaatgg actttttatt tctatttatg gagagactgt aactactgct tttaatgtct
     3781 ttttaaagca agctatttac cagcttgaaa atatcatgca tttaaaatag aatctatctg
     3841 gggagtctaa ttactattgt tcctatttga gccagttgtc tttcagtaaa gctatagaat
     3901 ctctcaaaga agtaaataga aatactactt cttaaatgtc ataggaagca tatccacttt
     3961 gtttctatta gtactttaag atctataaga ccttattact aaaaagtaga ggaatataaa
     4021 gaattcatca atttctgtat atgggcttta acctaatttt ccaggttatg cgacttataa
     4081 agcactgtga ccaaacttcc agagacagta aaaaaaaaaa aaagaaaaaa aagttttact
     4141 ccttcagaag tattgtcatg ctactgctgt acttattttc ctaatctctc tctatctgct
     4201 atactccctc cttttctttc tttttctttt aagcttttat atttattcct tcctttgttt
     4261 tcctctctgt aagacagcta ctactgagca ctaattagta gaaagtcaga gtggaggtta
     4321 tctgtgcatg tctttctgca aacattactg ttgaggccat ctgaagagag ggattgatag
     4381 gttaggctgt tgcaccgtga atgtcctgct ttgcattttt aacctcagct ccccaacata
     4441 tatatcttgc agtttatatg ccttttcatt taaccgaaac ctcttttatt taatttttaa
     4501 cataacaacc tacaattctt gtgtttttat tatgctgtat ttttctgtgt ttcttattac
     4561 atgtgattgc taaataaatc atatttgagg gaaaacattg tgaaacattt ggtgttttat
     4621 gcaattcaat ttagtgaggc attgaaaagt tggagaaatt gggggaaacc caaaggagac
     4681 caacttaaaa attgatatga gtttggtaag aaagtgcttt ttgttccatt aaataaatac
     4741 tgattgaatt acttattatt atacctatta ttcataagct cctggctgga agcatggtgc
     4801 atatatgcat atggcaatat ttcctctaga aatttcaagt cttataaagg agataatcta
     4861 cacagactgt aatttatacc taataactag aatgccacat aaaaaagtaa ccagttgcaa
     4921 ataaaatgca cctatgtata tttgaggagg taaaaaactg cttctgtctt cagagctttc
     4981 aggactaagt ggtgcatcag ttggcattta gtaaataaaa acaagtttat aaacatt
//
