LOCUS       NM_177966               8780 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens phosphodiesterase 12 (PDE12), transcript variant 1,
            mRNA.
ACCESSION   NM_177966
VERSION     NM_177966.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Wei,Q., Chen,L., Luo,W., Chen,C., Shi,Y., Xie,J., Xie,X. and
            Luo,H.B.
  TITLE     PDE12 disrupts mitochondrial oxidative phosphorylation and mediates
            mitochondrial dysfunction to induce oral mucosal epithelial barrier
            damage in oral submucous fibrosis
  JOURNAL   Eur J Pharmacol 967, 176353 (2024)
   PUBMED   38325798
  REMARK    GeneRIF: PDE12 disrupts mitochondrial oxidative phosphorylation and
            mediates mitochondrial dysfunction to induce oral mucosal
            epithelial barrier damage in oral submucous fibrosis.
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Tekin,H., Josefsen,K., Krogvold,L., Dahl-Jorgensen,K., Gerling,I.,
            Pociot,F. and Buschard,K.
  TITLE     PDE12 in type 1 diabetes
  JOURNAL   Sci Rep 12 (1), 18149 (2022)
   PUBMED   36307540
  REMARK    GeneRIF: PDE12 in type 1 diabetes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8780)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 8780)
  AUTHORS   Wood,E.R., Bledsoe,R., Chai,J., Daka,P., Deng,H., Ding,Y.,
            Harris-Gurley,S., Kryn,L.H., Nartey,E., Nichols,J., Nolte,R.T.,
            Prabhu,N., Rise,C., Sheahan,T., Shotwell,J.B., Smith,D., Tai,V.,
            Taylor,J.D., Tomberlin,G., Wang,L., Wisely,B., You,S., Xia,B. and
            Dickson,H.
  TITLE     The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of
            the Innate Immune Response and the Discovery of Antiviral
            Inhibitors
  JOURNAL   J Biol Chem 290 (32), 19681-19696 (2015)
   PUBMED   26055709
  REMARK    GeneRIF: PDE12 is a negative regulator of the antiviral innate
            immune response.
REFERENCE   5  (bases 1 to 8780)
  AUTHORS   Poulsen,J.B., Andersen,K.R., Kjaer,K.H., Vestergaard,A.L.,
            Justesen,J. and Martensen,P.M.
  TITLE     Characterization of human phosphodiesterase 12 and identification
            of a novel 2'-5' oligoadenylate nuclease - The ectonucleotide
            pyrophosphatase/phosphodiesterase 1
  JOURNAL   Biochimie 94 (5), 1098-1107 (2012)
   PUBMED   22285541
REFERENCE   6  (bases 1 to 8780)
  AUTHORS   Rorbach,J., Nicholls,T.J. and Minczuk,M.
  TITLE     PDE12 removes mitochondrial RNA poly(A) tails and controls
            translation in human mitochondria
  JOURNAL   Nucleic Acids Res 39 (17), 7750-7763 (2011)
   PUBMED   21666256
  REMARK    GeneRIF: 2'-phosphodiesterase is a mitochondrial protein that
            specifically removes poly(A) extensions from mitochondrial mRNAs
            both in vitro and in mitochondria of cultured cells.
REFERENCE   7  (bases 1 to 8780)
  AUTHORS   Poulsen,J.B., Andersen,K.R., Kjaer,K.H., Durand,F., Faou,P.,
            Vestergaard,A.L., Talbo,G.H., Hoogenraad,N., Brodersen,D.E.,
            Justesen,J. and Martensen,P.M.
  TITLE     Human 2'-phosphodiesterase localizes to the mitochondrial matrix
            with a putative function in mitochondrial RNA turnover
  JOURNAL   Nucleic Acids Res 39 (9), 3754-3770 (2011)
   PUBMED   21245038
  REMARK    GeneRIF: 2'-PDE locates to the mitochondrial matrix of human cells,
            and comprise an active 3'-5' exoribonuclease exhibiting a
            preference for oligo-adenosine RNA like canonical cytoplasmic
            deadenylases.
REFERENCE   8  (bases 1 to 8780)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8780)
  AUTHORS   Kohno,T., Yamaguchi,H. and Hakoshima,T.
  TITLE     Crystallization and preliminary X-ray crystallographic analysis of
            human phosphodiesterase 12
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun 66 (Pt 5), 520-522
            (2010)
   PUBMED   20445249
  REMARK    GeneRIF: crystals of phosphodiesterase 12 belonged to space group
            P3(1)21 or P3(2)21, with unit-cell parameters a = b = 111.3, c =
            192.4 A, and diffracted to 2.5 A resolution.
REFERENCE   10 (bases 1 to 8780)
  AUTHORS   Kubota,K., Nakahara,K., Ohtsuka,T., Yoshida,S., Kawaguchi,J.,
            Fujita,Y., Ozeki,Y., Hara,A., Yoshimura,C., Furukawa,H.,
            Haruyama,H., Ichikawa,K., Yamashita,M., Matsuoka,T. and Iijima,Y.
  TITLE     Identification of 2'-phosphodiesterase, which plays a role in the
            2-5A system regulated by interferon
  JOURNAL   J Biol Chem 279 (36), 37832-37841 (2004)
   PUBMED   15231837
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092418.3, AL831824.1 and U79276.1.
            
            On Nov 23, 2018 this sequence version replaced NM_177966.6.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the canonical protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.131458.1,
                                           SRR1803613.242445.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311180.9/ ENSP00000309142.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-469               AC092418.3         146608-147076
            470-2269            AL831824.1         1-1800
            2270-5433           AC092418.3         150672-153835
            5434-5479           U79276.1           1596-1641
            5480-8780           AC092418.3         153882-157182
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.3"
     gene            1..8780
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="phosphodiesterase 12"
                     /db_xref="GeneID:201626"
                     /db_xref="HGNC:HGNC:25386"
                     /db_xref="MIM:616519"
     exon            1..1414
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1936
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /EC_number="3.1.13.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; 2'-phosphodiesterase; 2',5'-phosphodiesterase
                     12; mitochondrial deadenylase"
                     /codon_start=1
                     /product="2',5'-phosphodiesterase 12 isoform 1 precursor"
                     /protein_id="NP_808881.3"
                     /db_xref="CCDS:CCDS33772.1"
                     /db_xref="GeneID:201626"
                     /db_xref="HGNC:HGNC:25386"
                     /db_xref="MIM:616519"
                     /translation="MWRLPGARAALRVIRTAVEKLSRAEAGSQTAAGAMERAVVRCVP
                     SEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNA
                     SGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERN
                     PPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSP
                     SSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGT
                     CTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNL
                     IQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFS
                     LLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRI
                     CVANTHLYWHPKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHF
                     VINGSIPEDHEDWASNGEEERCNMSLTHFFKLKSACGEPAYTNYVGGFHGCLDYIFID
                     LNALEVEQVIPLPSHEEVTTHQALPSVSHPSDHIALVCDLKWK"
     exon            1415..1493
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..8780
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3867..3872
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="hexamer: AATAAA"
     polyA_site      3889
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="major polyA site"
     regulatory      4980..4985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="hexamer: AATAAA"
     polyA_site      5004
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
     regulatory      8365..8370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="hexamer: CATAAA"
     polyA_site      8395
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
     regulatory      8757..8762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
                     /note="hexamer: AATAAA"
     polyA_site      8780
                     /gene="PDE12"
                     /gene_synonym="2'-PDE; 2-PDE"
ORIGIN      
        1 gcgagatctg aatgagtcaa agccggcggc ctcggctcct cagctccacc tgacagtagg
       61 ccgctgatcg gccgcgggtc ttgtcgaccg ctaggccacc aggttcatgt ggaggctccc
      121 aggcgcccgc gccgcgcttc gggtgatccg gacggcggtg gagaagctga gccgggctga
      181 agcggggagc cagacagcgg cgggagcgat ggagcgcgct gtagtgcgct gcgtaccttc
      241 ggaacccaag ctgagcctgt cattcgcttt ggctgatggt agccacaaga acatgcagcg
      301 cgaccagagc gagccgctgg gtcgagtcct cagccgcatc gctaccaatg ccctaaaggg
      361 tcacgctaag gcggccgccg ccaagaagag caggaagagc cggccgaatg ctagcggcgg
      421 tgcggcctgt tcagggccgg ggcctgagcc ggctgtgttc tgcgagcccg tggtgaagct
      481 gtactaccgg gaagaggcag tggctgagga cgtgctcaac gtggatgcct ggcaagacgg
      541 cgcggtgctg cagatcggcg atgttaagta caaggtggag cgcaacccgc ccgccttcac
      601 cgaactgcag ttgccgcgct acatcatggc cgggttccct gtgtgcccca aactcagcct
      661 cgaatttggg gatcccgcca gctccctttt ccgctggtat aaggaagcca agcccggagc
      721 ggcggagccc gaggtcggtg tcccctcgtc attgtctccc tcctcacctt cttcttcttg
      781 gactgagact gatgtggagg agcgtgtcta caccccgtcc aatgccgaca tcgggctaag
      841 gctcaagctt cactgcaccc caggcgatgg gcagcgcttt gggcacagcc gggagttgga
      901 aagtgtgtgt gtggtagagg ctgggcctgg cacctgcact tttgaccacc ggcatctcta
      961 cacgaagaag gtgactgagg acgctctcat ccgcactgtc tcttacaaca tcctggcaga
     1021 cacgtacgcg cagactgagt tctcgcgaac ggttctgtac ccatactgtg ccccctacgc
     1081 cctggagctc gactaccgcc agaaccttat ccagaaggaa ctcaccggct acaacgccga
     1141 tgtcatctgt ttgcaggagg ttgaccgcgc agtgttttct gacagcttgg tacccgccct
     1201 agaggccttc gggctcgagg gggtgtttcg aatcaagcag cacgaaggcc tggccacttt
     1261 ctaccgaaag tctaagttca gccttcttag ccagcatgac atttcattct acgaagccct
     1321 cgagtccgac ccacttcaca aagaactgct ggagaaacta gttttgtacc catcagcgca
     1381 ggagaaggtg ctccagagat cttctgttct tcaggtttca gttcttcagt ctacaaagga
     1441 ctcttctaaa aggatatgtg ttgctaatac ccatctttac tggcatccta aaggtgggta
     1501 tattcgcctc attcaaatgg cagtagcctt ggctcacata agacatgttt catgtgatct
     1561 gtatcctggc ataccagtta tattttgtgg ggactttaat agtacaccat caacaggaat
     1621 gtatcatttt gtcatcaatg gcagcattcc agaggatcat gaagactggg cttccaatgg
     1681 ggaggaggaa agatgcaata tgtctcttac acatttcttc aagctgaaaa gtgcttgtgg
     1741 tgaacctgct tacacaaatt atgttggtgg ctttcatgga tgtctagatt acattttcat
     1801 tgacttaaat gctttagagg ttgaacaggt gattccatta cctagtcatg aagaagttac
     1861 cacccaccag gccttaccta gtgtttccca tccctctgat cacatagcac ttgtatgtga
     1921 tttaaaatgg aaatagatgt gtgtttaatg gaattgaagt ctgaaaagga agtagttatt
     1981 ttagcagaaa atttaatatg aatcaaagct tatatgtaaa cttcaaggag gaatggtaaa
     2041 atgttcagcc ctcctagtta tgttcctgat gtcttcgtta tgaaactgtt gatgtttgca
     2101 tcatacatct tctctttcct tgttttcctc tacaattgga ggagaaacaa atatatttct
     2161 tactagcaaa atagaaaatt gaattatttt tctccaaatt gagactctca gaaaaggaag
     2221 attgaattag cgtgtttttt gtttgtttgt ttttgttttt gtttttgttt ttttgagatg
     2281 gagtttcact cttgttgccc aggctggagt gcaatggcac aatctcggct cactgcaacc
     2341 tccgccccct gggtttaagc gattctcctg cctcagcttc ccgagtagct gggattacag
     2401 gcgtgcgcca acatgtctgg ctaatttttg tatttttagt agaaatgggg tttcgccacg
     2461 ttggccaggc tggtcttgaa ctcctgacct caggtgatcc acccacctcg gcctcccaaa
     2521 gtgttgggat tacaggcgtg agccaccgca cccggccctt gtgtacattt ttataagaga
     2581 atttttttag ctaggagttc agaattttta aagtaccatt tgaatgatct taatttttct
     2641 ttcatgacaa cacattccaa aatgaatcat gcttatgtac taagagggaa aatgtattta
     2701 agttaagggt gagagactta agttataggt gaccttagag acctaaggtg agagacttga
     2761 cacatggaag gagtaacatt agggtctacc tctacctcaa tttagttagc gatttactac
     2821 aatttcagag ctttaacaaa agataaaaat aaatcgtcac caattgttat tgcttctcat
     2881 ctttcatttt tcaatgaaca agtaaggtat tttcattctt atttttagga ttttagtttt
     2941 tagtgtatgg tacaaatgaa cacagtttat attctaattc ttactgcagc tcattttaat
     3001 ttttaggatg caagcacaat ttagtattca aagtgagtag caacatattc aacttgatcc
     3061 cattgtcttc agttactctt gcccatgaaa aatgttcata aatgaacagg gtatttgacc
     3121 atatgatatt agaaaataca gcacattact ttatgagaaa ctacctactg atatgggctt
     3181 gaaattttgg atgaatcatt gagcatttct acactagaag taatttcaaa attgttggtt
     3241 tttataaaca ggaaaaaggt tgagtagtgg gacttttaag catctctgaa ataaaaaact
     3301 tctttttaca gacaagcatt atagtttgag ttacagacaa cagtgtgtat atatgtaata
     3361 tatatatagt aaaatgaaat ttaaatatga agccaaactt tttaaaatta gaaactacaa
     3421 atggttatac tgattagtgt ctagcctaga gtggtaacca tgctttacta attcagttat
     3481 gaaatacatt atttataatg cattagctgt attagctgtt gcttttttga tgttcaggat
     3541 aactatgtta tctcatttct gcatttaatt aatagctcga gtattaaaag cccactccct
     3601 tcaagaaaag ctttgatttt ccccagtcat gaaagccctt gtttcaaatt ctttaatctc
     3661 tgaacctagt atcataagaa tttcctcttt tgataacatc tgtactttca tattctgctc
     3721 actatcaaat gtattgttaa cacttagtaa gtttgaaaat gaaggggttt tatctgcatt
     3781 tgacattgaa ccttgaagta ctttaagtac tccaagggga aaattaaagt ggaagtttct
     3841 tcggatcttg tttagaaaaa actataaata aaaaattgat gctaccaaat tgtgccttcc
     3901 taaataacat ttttgagagc attttaacag cagtttacaa atatgtaaat aatagattaa
     3961 aaccaaatct tgattctctt gtgaattttt ttttcatttt aaaaatatgt ttttgggctg
     4021 ttttcaaaga aagatgttga tagaaccctt agagtgactt gggagaaaac aaagtgtcac
     4081 atcaaaaagt tgagaaacat tttgaaagaa aaaattcgaa catgcccatg aaaaaagcat
     4141 acagcttcca cattaacact ggctagatta aactctagtc agaaaaactc agcacttaac
     4201 agacattcca tgtcctatat ccttaatttt gatgttttct gactaaacaa tatactcaga
     4261 ttgtatacag gcatttcaca ttattaggac atggaatgtt attgtgaagt gccatatact
     4321 gtgatcaaat ctccagtata cggatacgtt aaaatgattg gagtccaaag gtagggaaca
     4381 ctaagaaaat gtataaatag catacacatt tctttgtact catgagttag aatgcagtgt
     4441 tattcagaaa tgtctgttta aagcacctat gtgattgcat ttttctatta catttggatt
     4501 ttttagtaca tttaatttta agaaaacatg ggaaatctag cgactttcta tggtaaacca
     4561 cattttataa tgtcacaagt tcttgctata ggaattaaca ttctagaatt ttatattgat
     4621 tataaaagag tatttcagaa catattattc tgttaaaatg aactcttgca cagttgccgc
     4681 tacagcaaat gtgtctgcca ataatcaaaa ttcatgagca aacaatcgtt tctggctgta
     4741 atgtatgttc tttttgaaat tctgtatgct aaaatcagtt gtttcaaaat ttcaagaatt
     4801 ccactgagta gtgatttttc tgagtactaa gctatgatct catcagaatg tgctggtttc
     4861 agtctggaga tagatttctg aggagcctaa tacaagggag agaatgtcca aagaacacta
     4921 ctgtggaaag ttcttacgat ggaaattgac atcttttgta tctcattctc cagccgtgaa
     4981 ataaaggcag aggttccctg caaactagtt tggttgggat aaatccaata tttatatata
     5041 atgaaaaata aaagtttgca ttattagttt atcacaagac ccatgtgaca actcagaaaa
     5101 tattctcagc ataatttttt taagagatag agtctcactg ttacccaggc tggagtgcag
     5161 tggtgcagtc ataacttact acagcctcaa actgcggggc tcaaatgatc ctcctgcctc
     5221 agcgtcccaa gtagttgaga ttacaagccc aaccttccat aactgaaaac taactttctt
     5281 tttttctttt tttattatta tactttaagt tctagtgtac atgtgcccaa cgtgcagatt
     5341 tgttacatag gtatacatgt gccatgttgg tttgctgcac ccgttaactc gtcatttaca
     5401 ttaggtattt ctcctaatgc tatccctccc ccagccctga aaatgaattt tttaaacaca
     5461 aataaaaaat atttcagtgt tttgagaatt ttaataattt gcttaatata gtacaggaag
     5521 gcaacactat ctagagggcc aggcatagtg gctcttgact gtaatcacaa cattttggga
     5581 ggctgaggca ggaggatcac ttgagctgag gagttcaaga ccagccttgg caacattgtg
     5641 agaccctatc tctgcaaaaa ataaaattaa ccaggcatgg tggtgcatgc ctgtagtccc
     5701 agctgctcag gaggctgagg ccagaggatc acttgagccc atgaggtcaa gcctgcagtg
     5761 agttgtgatc aaggtgcgct gcactccagc ctgggtaata gagcaagacc ctgtcttcaa
     5821 aaaaccactc tagatttcat gttgtagggg gtagggagga caatttttaa cagctttaac
     5881 tgacatgggt aatatataag ttctgtgttc tcactttata tgaaagtact tttttaaatg
     5941 ttaaaaatac tagagctgta ttaacttcgt gattttattt ttcttcttag cactaacttc
     6001 aaaataacca tacagtactg cttgtaattt ttgtattttt agtagagaca gggtttcacc
     6061 atattggcca ggctggtctt gaactcctga actcaagtga tctgcctgcc tcagcctctg
     6121 aaagtgctgg gattacaggc atgagccacg gtacccggcc taaattttaa ttttatatat
     6181 aaatggcaaa ataatcttaa atatagtaaa tctgttcacc tatctgctat gctagcaaat
     6241 tctgggttgt caaaaatcca tgttcatact gtcagtgtta gatttttact cataatttaa
     6301 aatttccccg caaaatttca cagaaaactg aattattcat ctctatcttc tgtccaattt
     6361 atgtaatgaa tgtatgtcaa agcatttata taacacagtc cattttgccc agtaattcac
     6421 tttaatgatg ttatcatgaa gtattgatgt actttttttt tagtgtattg aagtattcct
     6481 aggttgcatt tgtgctagtt tcataccatt tgtgttcctt atatattgaa gatggttcag
     6541 taggggaaat ggataggctt tatttagttg gctttcttat actggataat ataaacactt
     6601 tttttttctt ttttgagaca gggtctggct ttgtcaccca ggcttggagg gcactggtgc
     6661 catcacggct tactgcaacc tccacctcct gggcttatgc aatcctccca ccacagcctc
     6721 cccaaatagc tgggactaca ggtgcgcacc gccacacctg gctaattttt gtatatttta
     6781 taggcaaaaa aaaaaaaaaa aatggtttca ccatgttgcc caggctggtc ttgaaattct
     6841 cagttcaagc agtccaccca cctcggcctc ccaaagtgct gatattacag acatgagcca
     6901 ccccacccag tcagcacaca tttttttttt ttttaaataa gacagggtgt tgctgttgcc
     6961 caggctggag cgcagtggca tgatcatagc tcactgcagc atcaaactcc agggcttaag
     7021 cgatcctccc acctcagcct cctgagtagc tgggactata ggtgcatgcc accatgcctg
     7081 gctaattttt taaattgttt gtagagatgg gggtctctct atgttgccca gactggtctt
     7141 aaactcctgg gctcaaatga ctctcccgct tcagcctccc aaagtactgg gattataggt
     7201 gtgagccagc acacatagcc ctttttttaa aaaaattaaa aacaaatttt ttctccagaa
     7261 aaaaacatat ttatcaactt ttaaaaacat tttatgggta catagtaggt gtatatattt
     7321 atgtgggaca tatttctttt gatagagtaa attgagtatt catcacatca agcattcatt
     7381 tgtgttacga acattccaat tgtactctct cagttattct aaaatgtgat atacacactt
     7441 ttaaaaggat ttattgcgct gggcgcggtg gctcacgcct gtaatcccag cactctggga
     7501 ggctgaggcc ggtggatcat gaggtcagat cgagaccatc ctggccaaca tggtgaaacc
     7561 ctgtctctac taaaaataca aaaattagct gggcatggtg gcgcgcgcct gtagtctcag
     7621 ctgcttgggg aggctgaggc aggagaatca cttgaacccg ggaggcagag gttgcagtga
     7681 gccgagatca cgccattgca ctccagcctg gcaacaaagc gggactctgt ctcaaaaata
     7741 aaataaaata aaattttaaa aaaaggattt attgcatata attcttatat cccctgtgga
     7801 atagtttgga gaaatggtac atccaaagat aaaatcagtt agaaataaac agtggccttc
     7861 ctcagaaaag ctgggctata gtgttttggg tatcttaaac caagaaaggg tatttctatc
     7921 ccagctgtag agtttttaat aattctatta taaataatgg aatcaggcca gcacagtggc
     7981 tcacgtcaca taatcccact ctccttaaga gctttttttg tcactgcata tcaactcagt
     8041 tgacaaggac tggcccccaa aagaagctgc atactcactt ggcagtcact ccccattctc
     8101 cctagctcct ggcaacaact taacctgtct ctgtggattt gcctattctg gacatttcat
     8161 ataaattgaa tcataattta tgtagtcttt tgtgactggc ttcttttact taggatgttt
     8221 ttaaggttct tactgttgta ggatgtatca ggagtttgtt tttatgatga atgttccatc
     8281 ttatggacgt accacatttg tccattcatt agttgacaga catttgaatt gcttccactt
     8341 cctggcttct atgaataatg ctgccataaa catttgtgat aagtttttga gtgaatatgt
     8401 gtttttgttt ctcttgggca catacaggga atggaattgt tgggtgctgt gataaccttt
     8461 tgaggaactg ccagacttac ggaaagcagt tgcactattt tgcattccca ccaagcaata
     8521 tgagggttcc agtttccctc catcctcatt aacactttat tatctttttg attataggca
     8581 tcctagtgtc aagtggcatc ttattgtggt tttgatttgc atttccatga tggttaacga
     8641 tgttaagcat cttttcatgt gctcgttggc catctatata tctctggaga aatgtctttt
     8701 gaaatacttt gcccatttaa aaatgttatt tgttgtttag ccacccctcc ttaaaaaata
     8761 aataatataa taatccatca
//
