LOCUS       NM_001385079            9334 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens phosphodiesterase 10A (PDE10A), transcript variant 3,
            mRNA.
ACCESSION   NM_001385079
VERSION     NM_001385079.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Sano,Y., Yamamoto,Y., Kubota,M., Moriguchi,S., Matsuoka,K.,
            Kurose,S., Tagai,K., Endo,H., Yamagata,B., Suzuki,H., Tarumi,R.,
            Nomoto,K., Takado,Y., Kawamura,K., Zhang,M.R., Tabuchi,H.,
            Mimura,M., Uchida,H., Higuchi,M. and Takahata,K.
  TITLE     Alterations of striatal phosphodiesterase 10 A and their
            association with recurrence rate in bipolar I disorder
  JOURNAL   Transl Psychiatry 14 (1), 403 (2024)
   PUBMED   39358334
  REMARK    GeneRIF: Alterations of striatal phosphodiesterase 10 A and their
            association with recurrence rate in bipolar I disorder.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Kalampokini,S., Xiromerisiou,G., Bargiotas,P., Anastasiadou,V.C.,
            Costeas,P. and Hadjigeorgiou,G.M.
  TITLE     PDE10A Mutation as an Emerging Cause of Childhood-Onset
            Hyperkinetic Movement Disorders: A Review of All Published Cases
  JOURNAL   Neuropediatrics 55 (4), 217-223 (2024)
   PUBMED   38442915
  REMARK    GeneRIF: PDE10A Mutation as an Emerging Cause of Childhood-Onset
            Hyperkinetic Movement Disorders: A Review of All Published Cases.
            Review article
REFERENCE   3  (bases 1 to 9334)
  AUTHORS   Bohlega,S., Abusrair,A.H., Al-Qahtani,Z., Guzman-Vega,F.J.,
            Ramakrishnan,R., Aldosari,H., Aldakheel,A., Al-Qahtani,S.,
            Monies,D. and Arold,S.T.
  TITLE     Expanding the genotype-phenotype landscape of PDE10A-associated
            movement disorders
  JOURNAL   Parkinsonism Relat Disord 108, 105323 (2023)
   PUBMED   36805523
  REMARK    GeneRIF: Expanding the genotype-phenotype landscape of
            PDE10A-associated movement disorders.
REFERENCE   4  (bases 1 to 9334)
  AUTHORS   Borneman,R.M., Gavin,E., Musiyenko,A., Richter,W., Lee,K.J.,
            Crossman,D.K., Andrews,J.F., Wilhite,A.M., McClellan,S., Aragon,I.,
            Ward,A.B., Chen,X., Keeton,A.B., Berry,K., Piazza,G.A.,
            Scalici,J.M. and da Silva,L.M.
  TITLE     Phosphodiesterase 10A (PDE10A) as a novel target to suppress
            beta-catenin and RAS signaling in epithelial ovarian cancer
  JOURNAL   J Ovarian Res 15 (1), 120 (2022)
   PUBMED   36324187
  REMARK    GeneRIF: Phosphodiesterase 10A (PDE10A) as a novel target to
            suppress beta-catenin and RAS signaling in epithelial ovarian
            cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9334)
  AUTHORS   Luo,L., Cai,Y., Zhang,Y., Hsu,C.G., Korshunov,V.A., Long,X.,
            Knight,P.A., Berk,B.C. and Yan,C.
  TITLE     Role of PDE10A in vascular smooth muscle cell hyperplasia and
            pathological vascular remodelling
  JOURNAL   Cardiovasc Res 118 (12), 2703-2717 (2022)
   PUBMED   34550322
  REMARK    GeneRIF: Role of PDE10A in vascular smooth muscle cell hyperplasia
            and pathological vascular remodelling.
            Erratum:[Cardiovasc Res. 2023 May 2;119(3):879. doi:
            10.1093/cvr/cvac011.. PMID: 35137003]
REFERENCE   6  (bases 1 to 9334)
  AUTHORS   Fujishige,K., Kotera,J., Yuasa,K. and Omori,K.
  TITLE     The human phosphodiesterase PDE10A gene genomic organization and
            evolutionary relatedness with other PDEs containing GAF domains
  JOURNAL   Eur J Biochem 267 (19), 5943-5951 (2000)
   PUBMED   10998054
REFERENCE   7  (bases 1 to 9334)
  AUTHORS   Kotera,J., Fujishige,K., Yuasa,K. and Omori,K.
  TITLE     Characterization and phosphorylation of PDE10A2, a novel
            alternative splice variant of human phosphodiesterase that
            hydrolyzes cAMP and cGMP
  JOURNAL   Biochem Biophys Res Commun 261 (3), 551-557 (1999)
   PUBMED   10441464
REFERENCE   8  (bases 1 to 9334)
  AUTHORS   Fujishige,K., Kotera,J., Michibata,H., Yuasa,K., Takebayashi,S.,
            Okumura,K. and Omori,K.
  TITLE     Cloning and characterization of a novel human phosphodiesterase
            that hydrolyzes both cAMP and cGMP (PDE10A)
  JOURNAL   J Biol Chem 274 (26), 18438-18445 (1999)
   PUBMED   10373451
REFERENCE   9  (bases 1 to 9334)
  AUTHORS   Loughney,K., Snyder,P.B., Uher,L., Rosman,G.J., Ferguson,K. and
            Florio,V.A.
  TITLE     Isolation and characterization of PDE10A, a novel human 3',
            5'-cyclic nucleotide phosphodiesterase
  JOURNAL   Gene 234 (1), 109-117 (1999)
   PUBMED   10393245
REFERENCE   10 (bases 1 to 9334)
  AUTHORS   Loughney,K., Martins,T.J., Harris,E.A., Sadhu,K., Hicks,J.B.,
            Sonnenburg,W.K., Beavo,J.A. and Ferguson,K.
  TITLE     Isolation and characterization of cDNAs corresponding to two human
            calcium, calmodulin-regulated, 3',5'-cyclic nucleotide
            phosphodiesterases
  JOURNAL   J Biol Chem 271 (2), 796-806 (1996)
   PUBMED   8557689
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121789.38, KF458359.1,
            AL160160.14, AL136130.7 and AL117345.22.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The protein encoded by this gene belongs to the cyclic
            nucleotide phosphodiesterase family. It plays a role in signal
            transduction by regulating the intracellular concentration of
            cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP
            to the corresponding nucleoside 5' monophosphate, but has higher
            affinity for cAMP, and is more efficient with cAMP as substrate.
            Alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (3) encodes the longest isoform
            (3). It uses upstream transcription and translation start sites,
            compared to variant 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF127480.1, ERR4352443.747412.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000539869.4/ ENSP00000438284.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1224              AL121789.38        67382-68605         c
            1225-1295           KF458359.1         121-191             c
            1296-1424           AL160160.14        36060-36188         c
            1425-1453           AL136130.7         25138-25166
            1454-1574           AL136130.7         57119-57239
            1575-1624           AL136130.7         58504-58553
            1625-1765           AL117345.22        2530-2670
            1766-1921           AL117345.22        4778-4933
            1922-1972           AL117345.22        6435-6485
            1973-2031           AL117345.22        7562-7620
            2032-2083           AL117345.22        9198-9249
            2084-2226           AL117345.22        19130-19272
            2227-2319           AL117345.22        21626-21718
            2320-2506           AL117345.22        24220-24406
            2507-2649           AL117345.22        41448-41590
            2650-2733           AL117345.22        42643-42726
            2734-2884           AL117345.22        45111-45261
            2885-3040           AL117345.22        49454-49609
            3041-3213           AL117345.22        58541-58713
            3214-3325           AL117345.22        94405-94516
            3326-3406           AL117345.22        98549-98629
            3407-3495           AL117345.22        101696-101784
            3496-9334           AL117345.22        104780-110618
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..9334
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /note="phosphodiesterase 10A"
                     /db_xref="GeneID:10846"
                     /db_xref="HGNC:HGNC:8772"
                     /db_xref="MIM:610652"
     exon            1..1295
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     CDS             431..3598
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /EC_number="3.1.4.17"
                     /note="isoform 3 is encoded by transcript variant 3; cAMP
                     and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase
                     10A; phosphodiesterase 10A1 (PDE10A1); dJ416F21.1
                     (phosphodiesterase 10A); phosphodiesterase 10A7 (PDE10A7);
                     3',5' cAMP/cGMP phosphodiesterase"
                     /codon_start=1
                     /product="cAMP and cAMP-inhibited cGMP 3',5'-cyclic
                     phosphodiesterase 10A isoform 3"
                     /protein_id="NP_001372008.1"
                     /db_xref="CCDS:CCDS94029.1"
                     /db_xref="GeneID:10846"
                     /db_xref="HGNC:HGNC:8772"
                     /db_xref="MIM:610652"
                     /translation="MASLEEPLAPRPQGPLPAAGDEPGCGPGKLRPEPRLSAAGGGSA
                     AGPGPAPEWPGRGRAERAAPPRPPLSSAGRPSPAGGPGALSARGGGCGWVAARAPLAL
                     AFSSRVPSSSPSFFYFWPPPPPPPPSFLPSSSAFHLPVRLPGREGAAAAAAAGGGGDA
                     GGGGGGGQEAAPLSVPTSSSHRGGGGSGGGRRRLFLSPALQGLLLPARAGPRPPPPPR
                     LPLGQAARRAGSPGFPGAGPGGGGQTPRRPQGASFALAAAAALLFGSDMEDGPSNNAS
                     CFRRLTECFLSPSLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKNNKSEDES
                     APKEVSRYQDTNMQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYF
                     LGECNNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGDERFP
                     RGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQEVATANLAWASV
                     AIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKNLVNADRCALF
                     QVDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVLNIPDAYA
                     DPRFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAV
                     FCALALHCANMYHRIRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIEL
                     FHFDIGPFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVT
                     VAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTST
                     MEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEM
                     YQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKL
                     GIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEK
                     VIRGEETATWISSPSVAQKAAASED"
     exon            1296..1424
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1453
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1574
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1624
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1765
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1921
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..1972
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2031
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2083
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2226
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2319
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2506
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2649
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..2733
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2884
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3040
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3213
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3325
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            3326..3406
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            3407..3495
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..9334
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9111..9116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /note="hexamer: AATAAA"
     polyA_site      9138
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
     regulatory      9315..9320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /note="hexamer: AATACA"
     polyA_site      9334
                     /gene="PDE10A"
                     /gene_synonym="ADSD2; HSPDE10A; IOLOD; LINC00473;
                     PDE10A19"
                     /note="major polyA site"
ORIGIN      
        1 agtgggcgtc ttctgccgcc gccgccacca ccgcctccac gggcagcggc actgcggcgc
       61 ttttctccgc tgctttcgag aagccgccga ggctcagccg ccggagcgcg cacacaccac
      121 gccacgcctg cagggagggt ctccgccgtc cctagccccg gcccgggagg acgccgattg
      181 tgcgcgcctg agggtagcca ggcgccgtcg ccggggcctg gcccccaggc ccgggtcctc
      241 ccgcaaccca agccgagcac aatgtgaaga ctagactggg agggaagccg aagaccggag
      301 aggaggacca ggcagcggtt cgggcggccg gcaggggtgg gcaggtcctg cgggggcgga
      361 gcccaccgag ccccggcccg ccgcgcccct cccgcccgcg gcctgcccct ccgcggcccg
      421 gggaggaacc atggccagcc tcgaggagcc tcttgctccc cgcccccagg gtcccctgcc
      481 agcggccggg gacgagccgg gctgcggccc cggcaaactc cgcccggaac cccggctcag
      541 cgcggccggc ggggggagcg cggcgggccc gggcccggcc cccgagtggc ctggccgtgg
      601 ccgggcggag cgcgccgcgc ccccgcgccc gcccctctcc tccgcaggcc gccccagccc
      661 cgctgggggc cccggcgccc tctctgcgcg cggcggcggc tgcggctggg tcgcggcccg
      721 cgctcccttg gcgctcgcct tctcgtcccg ggtcccctcc tcctctccat ctttctttta
      781 cttctggccg ccgccccccc cgccccctcc ctcttttctc ccctcctcat ctgcctttca
      841 cctccccgtg cgtctcccag gaagggaggg cgcagcggcg gcggcggcgg cgggaggagg
      901 cggcgatgct ggcggaggag gaggaggagg acaagaggca gctcccttga gcgtccccac
      961 cagcagtagt caccgcggcg gcggtggcag cggcggcggg cggcggcggc tcttcctctc
     1021 gcctgcgctc cagggcttgc tgcttccagc ccgtgccggt ccccggccgc cgccgccgcc
     1081 ccggctcccc cttggccagg ccgcccgccg cgcgggctcc cccggcttcc ccggcgccgg
     1141 cccaggcggc ggcggccaaa ctccgcggcg tccccagggc gccagcttcg ccctcgccgc
     1201 cgcggccgcg ctgctcttcg gctccgacat ggaagatgga ccttctaata atgcgagctg
     1261 cttccgaagg ctgaccgagt gcttcctgag ccccagtttg acagatgaaa aagtgaaggc
     1321 atatctttct cttcaccccc aggtattaga tgaatttgta tctgaaagtg ttagtgcaga
     1381 gacagtagag aaatggctga agaggaagaa caacaaatca gaagatgaat cagctcctaa
     1441 ggaagtcagc aggtaccaag atacgaatat gcagggagtt gtatatgaac taaacagcta
     1501 tatagaacaa cggttggaca caggaggaga caaccagcta ctcctctatg aactgagcag
     1561 catcattaaa atagccacaa aagccgatgg atttgcactg tatttccttg gagagtgcaa
     1621 taatagcctg tgtatattca cgccacctgg gataaaggaa ggaaaacccc gcctcatccc
     1681 tgctgggccc atcactcagg gcaccaccgt ctctgcttat gtggccaagt ccaggaaaac
     1741 actgctagta gaagacatcc ttggagatga acgatttcca agaggtactg gactggaatc
     1801 agggactcgt atccagtctg ttctttgctt accaattgtc actgcaattg gtgacttgat
     1861 tggtattctc gagctgtatc ggcactgggg caaagaagcc ttctgtctta gtcaccagga
     1921 ggttgcaaca gcaaatcttg cctgggcttc agtagcaata catcaggtgc aggtatgcag
     1981 aggccttgcc aaacagacag aattgaatga cttcctactc gacgtatcaa aaacatattt
     2041 tgataacata gttgcaatag attctctact tgaacacata atgatatatg caaaaaacct
     2101 ggtgaatgcc gatcgttgtg cgcttttcca ggtggaccat aagaacaagg agttatattc
     2161 agaccttttt gatattggag aggaaaagga aggaaaacct gtcttcaaga agaccaaaga
     2221 gataagattt tcaattgaga aaggaattgc tggccaagta gcaagaacag gggaagtcct
     2281 gaacattcca gatgcctatg cagacccacg ctttaacaga gaagtagact tgtacacagg
     2341 ctacaccacg cggaacatcc tgtgcatgcc catcgtcagc cgaggcagcg tgataggtgt
     2401 ggtgcagatg gtcaacaaaa tcagtggcag tgccttctct aaaacagatg aaaacaactt
     2461 caaaatgttt gccgtctttt gtgctttagc cttacactgt gctaatatgt atcatagaat
     2521 tcgccactca gagtgcattt accgggtaac gatggaaaag ctgtcctacc atagcatttg
     2581 tacttcagaa gagtggcaag gtctcatgca attcaccctt cccgtgcgtc tctgcaaaga
     2641 aattgaatta ttccactttg acattggtcc ttttgaaaac atgtggcctg gaatttttgt
     2701 ctacatggtt catcggtcct gtgggacatc ctgctttgag cttgaaaagt tgtgtcgttt
     2761 tattatgtct gtgaagaaga actatcggcg ggttccttat cacaactgga agcatgcggt
     2821 cactgtagca cactgcatgt atgccatact tcagaacaat cacacgcttt tcacagacct
     2881 tgagcgcaaa ggactgctga ttgcgtgtct gtgtcatgac ctggaccaca ggggcttcag
     2941 taacagctac ctgcagaagt tcgaccaccc tctggccgct ctctactcca cttccaccat
     3001 ggagcagcac cacttctccc agactgtgtc catcctccag ttggaagggc acaatatctt
     3061 ctccactctg agctccagtg aatatgagca ggtgcttgag atcatccgca aagccatcat
     3121 tgccacagac cttgctttat actttggaaa caggaagcag ttggaagaga tgtaccagac
     3181 cggatcacta aaccttaata atcaatcaca tagagaccgt gtaattggtt tgatgatgac
     3241 tgcctgtgac ctttgttctg tgacaaaact gtggcccgtt acaaaattga cggcaaatga
     3301 tatatatgca gaattctggg ctgagggtga tgaaatgaag aaattgggaa tacagcctat
     3361 tcctatgatg gacagagaca agaaggatga agtcccccaa ggccagcttg ggttctacaa
     3421 tgccgtggcc attccctgct atacaaccct tacccagatc ctccctccca cggagcctct
     3481 tctgaaagca tgcagggata atctcagtca gtgggagaag gtgattcgag gggaggagac
     3541 tgcaacctgg atttcatccc catccgtggc tcagaaggca gctgcatctg aagattgagc
     3601 actggtcacc ctgacacgct gtcccaccta cagatcctca tcttgcttct ttgacattct
     3661 tttccttttt ttgggggggg tggggggaac ctgcacctgg taactggggt gcaaacctct
     3721 tcaagaaggt aacatcaaat aaataagtca agcagaggac ttcctgccaa tctcttctgt
     3781 gaggcatcat agacactgag caaccaggac cacccccacg ttcagaaatc agctggccaa
     3841 gtgactccat ttgacttgca aaccagcctt ttctaatagg ctaatattgc tgaggcctta
     3901 aaggaaatgg acaaaaatta tccagaaggg gtacttttcc attgtatctt tctaataagg
     3961 gtttaaaatg gtactattat ggtattgtac ttgggcttta acatcaatgt tgctttgatg
     4021 ttgttggata taaataggaa tttttacaca ttactattgt gaatggtgaa tgttcatgta
     4081 tgacctactt gtaattaact tgagttgtag tccacagcct caggacaaat gtcgttgagg
     4141 ttacagagta agaaatgatg gcaaaacgtc aaactcttat ttcagagctt catgaattta
     4201 gttagactaa acataattct ttaagttcaa cctaaagggc tgagatcaat aaatttaaca
     4261 ctagacgaag tagacttcct gtctttttga gaagagatga ggtatatgtt acaataaatc
     4321 tcagaacttc aagtagcagt tcaaaagatg tcagttttta aaattgtttt tgttgttgtc
     4381 ttggcagttt tactgaaccc tttgcataaa gaacaaaata aaagctcggc attgtaattt
     4441 ttttaatgga caagtcttat ggatacgaag ggtacatttt tcataatgat tcctttatat
     4501 tttcactttg tgtcattgca gaattttaga ctctcattca caatgaaaag tttattttaa
     4561 acattgttta attaaaatac catacagttc tcttttaaac atcaaaccat aaaaagtgta
     4621 ttttgtaatt ttactctgac ctgccgcagt cacctctcac ttatctcttc cacgtactgc
     4681 acggtcgtat ttcatgagct ttctgtccat agcacagaaa cagagcagaa agtagtacaa
     4741 tcatgttgga ccttctttct gttctcttta ctcttctcac agatcagatc actccataga
     4801 agcctgtggg tttcgatggt ttcttctata cacctttttg gttgaccagt attactatac
     4861 aatgtaagtg ttttaaaaaa tacgaaagta atactctgca ccccttccta caaagatgat
     4921 aaagcagtca cttctggcgc attttaataa tttaaagatt tttagtgcaa tggcacggta
     4981 acctccaaac ctgaattaga cagagactca ctcaggaagt gacaggccca tcatatcaaa
     5041 taacttattc acttttcatg tggcaggaaa ctggaatatc gcttttaata aaatggaaaa
     5101 atatgcttct acatatttac caccataggc gttttgttca tatgagcctg gtttgtgcaa
     5161 aattaaatca gaggcttcta caacatggtt tatttatgtt gtagcaaagt tggctctaca
     5221 taaacattgt tcttatttta aaattaacac tatgtgttca gttttcttgt gggcttctga
     5281 aagttgccat cttccctccg tggagctcca tttgctattt tcattataca ctatgaggta
     5341 aaatgtaata acaaaagaga gagaagtacc actgtggcta gatatataca cacacatata
     5401 tatatggatg gatgtaatat atgtagaaca cacacataga tgtatatagg atacacactc
     5461 atgtatgtaa acgtatacat atgtgtatat atgatacata cacatacaca cacacgagag
     5521 acagaaggaa agagaggaag agagaagcaa acatgtagga aaaaatataa atcagcccca
     5581 ttctcccatt ctttaagaac aatgattttt cttgatggaa aatgcatatc acttgacagt
     5641 tgacaagaaa gtgtcatttt tgtgttagtg tatcatataa ttgaatttga acaagataca
     5701 tatttttctg catacaaatt tatgaagctg gagtctacca aacacagctt tagtcacaaa
     5761 agttagttta gttatctcat ttcgcttgga gatggaattt caggaaggga tatattattt
     5821 aagattattg tttaattaca agaattttaa aagaatgtat gaattctgtt ttttaatata
     5881 gaaaataatt atggagttag gaatctcatg gctacattta catttttagg ttattacaga
     5941 aaattctctt aatttattat agtcaagtgt attatgtaat ctctatttag aacagtttcc
     6001 caaaggaatg tgattggatt tactggttgg ttaatttctt gtgaaatatt gtcataatag
     6061 aagcacagct tgaacaaaag actgaattct gatataaact gtcaatattg cttttaccat
     6121 gtgtaagatt tacttagtaa tcacagattt acctaaaata aaaggacccg ggttgcagtg
     6181 atatttagtt aatgatttgt tttggaaaac atgcagttag gagtattttt gtattttgtc
     6241 agatgtttag gagacagact ttcgggtcat gtttcttgtc ttgttcccag atcttttctt
     6301 aaaagaacag tgtgacacca gaagccaaga gatggttttt actaacaaca gctttgatga
     6361 gttcctatta aggtcgtatt gtgtattgtg ccaaattttc cacccagtat aaatttagta
     6421 acccgctatg agcatatgta ggatattcaa tttgcagata cttttgttgg ttatgttggt
     6481 ttataataga caagttttat ggtcatatgc aattattcca ttatcttttt ggacacagtg
     6541 aatatgtttt ttagctaaaa atgtccttgg aaacataaat gcctgccaat aacatggaat
     6601 attgaattcg ggcaatgtga attgattaat tagaaaaggg tcaaaagaaa tacaatggat
     6661 gtggaaacta attctaaaaa atagccctat taatagggca gagactctgg tccatttccc
     6721 agctgtactt ctctctaaga ggtgtatgaa aaacccatct tgcaaaagag accaaaatat
     6781 tagacacaat cgttcccatt cctgactctt caatggaaaa ggaaggtaca gatgtttaca
     6841 gtggtgcccc aggtgactcg ttctcaccac aacaaaagca actaattgga aactagatgt
     6901 gttcaataat agatttcctc ttttttggca tgctttttag attttttttc attttgtaaa
     6961 atctcagtta atttttcttt attggtatat ttgtgtatgc gagacaaatc ctgtattgta
     7021 caggatataa atagatgcat aaatccttag atacctgggt tttttaatag gtttaggttt
     7081 gggtattgtg tttctggtct tttaactcaa aattaaggtt tgagctttaa taggactaaa
     7141 ttattcacct gccttggcct gtgcctttat tttaaatact gcaattttac ttcttcattc
     7201 atgtatttat tattttgctt ttcttgctta agtataattg aaaatatttg caggaacgca
     7261 caattcattt tatcaagttg aatgaatagg accattctct cctcatcaga tttctgttct
     7321 ttatttgact tcattgacat ataccctggt gactataacc agtgaactgt tcagagtcag
     7381 tcttttcggc aagaacttca gagaactgtg acactggtta tgatgtgatt ttggggggtt
     7441 tcaggtacct aagcatgatt tttaaaatca cttctctctt tacatatttc gaacatatgt
     7501 ttgctttcta cactttgcag attttattta ggatttaatt ctttaaagac tatattcatt
     7561 tagtcactat agtggatctc ttctactgca agtattcatc tgatttttaa attatgtaaa
     7621 atgtaaatca gccaagtatg tcatccgtta tagtttcccc atgctttcag cgcatggcca
     7681 tgagattttt acatgtgcat aagtatttag ccaatacaca ttttgctgac ttctttaacc
     7741 atttatattg tttaaattag taatatccaa tgcagtgcac acttttttgt cttttttcca
     7801 acggaatatt ttggttgtct gtagcgttgg aagcatttta tatgataaaa atagcaagaa
     7861 aaaattaaaa agtgtcaacg gtgtgctggt tctaaattaa gaagatagaa caaagagaaa
     7921 gacattaatg ttacctaaga aacacattta acatccgaag aagcaatgct gtcctcactt
     7981 gatgtttctc ttctcaaccc agcaatggag agcagggctt gcaaagccgt acagagccta
     8041 cgtgctcttt cctgcaagtg ggagaaaact aatcaatggc acacagggcc aggtgtggcc
     8101 ctgtgaggca aaggataatg tcacctcagg accaaggtgg aaacttacag ctgaatccag
     8161 ggcttactaa gtagggaagt cagtttacat atttcagttt tcctctgatg tttttgaggt
     8221 actgtgtatt tgcagaagtt taagcagaag ggttttgttt ctattgtaag ttaggttcta
     8281 aaagtgtata atctgatact ctagcccaac tgaaatatta tatcttgggc gttacagcct
     8341 ttgtcttttt ctttagtcac cttaataccc agtcctatgc tgtatctctg atagcctata
     8401 taaatgacat ataggaatat tattttccat atgatctgtt gtatggtgta tatgctatgt
     8461 gttcatttcg tgtgcaagtt cagagtgtaa tagatttacc cagagaaggt gtttctatac
     8521 aatgccccta cctatttgaa attacatgct tgcccaacac actgtgaaat agttaccaaa
     8581 atttgtacaa atgcagcatc ttcattcttt ctgagaagac aagatggttt tctttacatg
     8641 aacaaatgaa caaaagagat cctagatcca taacgtagct aaggcatcta agagtttgct
     8701 gttgataatc ttgctgacca aaaactactg gagagtaaca caggttatat gccatcacaa
     8761 atacaatgct catgaagaac tgatttgtag agtcaatgaa cctgtgtcca gaattttaat
     8821 aggctctcta ttggaaggag aaagaatttc aagttaacag tatctaactt tatcatagtt
     8881 gatgttagta aattttaaaa aatgatttta tatgtatgac aaaaatcttt gtaaaatgcg
     8941 caagtgcaat aatttaaaga ggtcttaact ttgcatttat aaattataaa tattgtacat
     9001 gtgtgtaatt ttttcatgta ttcatttgca gtctttgtat ttaaaaaaca tttactgtta
     9061 tgtttgtata atagaacagt aatcatttat tataatctag gcaagttgta aataaattca
     9121 taattcaaac agccagtata tatgcatata tgagtgttat attgcaaaat ctttgtttta
     9181 cttacatggt taaagcagca agaattcttt tgttgatatg taattataca cataaaatat
     9241 atatatgtat gatacatgaa atatatttag aaatgttcat aattttaatg gatatccttt
     9301 ggtgtgaata attgaataca gagtttttaa aata
//
