LOCUS       NM_001312673            5546 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens phosphate cytidylyltransferase 1A, choline (PCYT1A),
            transcript variant 2, mRNA.
ACCESSION   NM_001312673
VERSION     NM_001312673.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Mao,Z., Li,T., Zhao,H., Wang,X., Kang,Y. and Kang,Y.
  TITLE     Methylome and transcriptome profiling revealed epigenetic silencing
            of LPCAT1 and PCYT1A associated with lipidome alterations in
            polycystic ovary syndrome
  JOURNAL   J Cell Physiol 236 (9), 6362-6375 (2021)
   PUBMED   33521992
  REMARK    GeneRIF: Methylome and transcriptome profiling revealed epigenetic
            silencing of LPCAT1 and PCYT1A associated with lipidome alterations
            in polycystic ovary syndrome.
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5546)
  AUTHORS   Yu,J., Wu,C., Wu,Q., Huang,J., Fu,W., Xie,X., Li,W., Tang,W., Xu,C.
            and Jin,G.
  TITLE     PCYT1A suppresses proliferation and migration via inhibiting mTORC1
            pathway in lung adenocarcinoma
  JOURNAL   Biochem Biophys Res Commun 529 (2), 353-361 (2020)
   PUBMED   32703435
  REMARK    GeneRIF: PCYT1A suppresses proliferation and migration via
            inhibiting mTORC1 pathway in lung adenocarcinoma.
REFERENCE   4  (bases 1 to 5546)
  AUTHORS   Yue,L., McPhee,M.J., Gonzalez,K., Charman,M., Lee,J., Thompson,J.,
            Winkler,D.F.H., Cornell,R.B., Pelech,S. and Ridgway,N.D.
  TITLE     Differential dephosphorylation of CTP:phosphocholine
            cytidylyltransferase upon translocation to nuclear membranes and
            lipid droplets
  JOURNAL   Mol Biol Cell 31 (10), 1047-1059 (2020)
   PUBMED   32186954
  REMARK    GeneRIF: Differential dephosphorylation of CTP:phosphocholine
            cytidylyltransferase upon translocation to nuclear membranes and
            lipid droplets.
REFERENCE   5  (bases 1 to 5546)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5546)
  AUTHORS   Weinhold,P.A. and Feldman,D.A.
  TITLE     Fatty acids promote the formation of complexes between
            choline-phosphate cytidylyltransferase and cytidylyltransferase
            binding protein
  JOURNAL   Arch Biochem Biophys 318 (1), 147-156 (1995)
   PUBMED   7726555
REFERENCE   7  (bases 1 to 5546)
  AUTHORS   Kalmar,G.B., Kay,R.J., LaChance,A.C. and Cornell,R.B.
  TITLE     Primary structure and expression of a human CTP:phosphocholine
            cytidylyltransferase
  JOURNAL   Biochim Biophys Acta 1219 (2), 328-334 (1994)
   PUBMED   7918629
REFERENCE   8  (bases 1 to 5546)
  AUTHORS   Johnson,J.E. and Cornell,R.B.
  TITLE     Membrane-binding amphipathic alpha-helical peptide derived from
            CTP:phosphocholine cytidylyltransferase
  JOURNAL   Biochemistry 33 (14), 4327-4335 (1994)
   PUBMED   8155650
REFERENCE   9  (bases 1 to 5546)
  AUTHORS   Hunt,A.N., Normand,C.S. and Postle,A.D.
  TITLE     CTP:cholinephosphate cytidylyltransferase in human and rat lung:
            association in vitro with cytoskeletal actin
  JOURNAL   Biochim Biophys Acta 1043 (1), 19-26 (1990)
   PUBMED   2155660
REFERENCE   10 (bases 1 to 5546)
  AUTHORS   Vance,J.E. and Vance,D.E.
  TITLE     Does rat liver Golgi have the capacity to synthesize phospholipids
            for lipoprotein secretion?
  JOURNAL   J Biol Chem 263 (12), 5898-5909 (1988)
   PUBMED   2833521
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK309073.1, L28957.1,
            AK304534.1, DB223595.1 and AC069257.28.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001312673.1.
            
            Summary: This gene belongs to the cytidylyltransferase family and
            is involved in the regulation of phosphatidylcholine biosynthesis.
            Mutations in this gene are associated with spondylometaphyseal
            dysplasia with cone-rod dystrophy. Alternatively spliced transcript
            variants have been found for this gene. [provided by RefSeq, Aug
            2015].
            
            Transcript Variant: This variant (2) lacks a 5' non-coding exon,
            thus has a shorter 5' UTR compared to variant 1. Variants 1 and 2
            encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.80988.1,
                                           SRR1803616.271858.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000431016.6/ ENSP00000394617.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                AK309073.1         27-103
            78-872              L28957.1           1-795
            873-1703            AK304534.1         756-1586
            1704-2051           DB223595.1         220-567
            2052-5546           AC069257.28        142070-145564
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..5546
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /note="phosphate cytidylyltransferase 1A, choline"
                     /db_xref="GeneID:5130"
                     /db_xref="HGNC:HGNC:8754"
                     /db_xref="MIM:123695"
     exon            1..112
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            113..239
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1226
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /EC_number="2.7.7.15"
                     /note="phosphorylcholine transferase A; CTP:phosphocholine
                     cytidylyltransferase A; CT A; CCT A; CCT-alpha;
                     CTP:phosphocholine cytidylyltransferase-alpha;
                     choline-phosphate cytidylyltransferase alpha;
                     phosphorylcholine transferase alpha; phosphate
                     cytidylyltransferase 1, choline, alpha"
                     /codon_start=1
                     /product="choline-phosphate cytidylyltransferase A"
                     /protein_id="NP_001299602.1"
                     /db_xref="CCDS:CCDS3315.1"
                     /db_xref="GeneID:5130"
                     /db_xref="HGNC:HGNC:8754"
                     /db_xref="MIM:123695"
                     /translation="MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQP
                     APFSDEIEVDFSKPYVRVTMEEASRGTPCERPVRVYADGIFDLFHSGHARALMQAKNL
                     FPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAE
                     HRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYAR
                     RNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQ
                     KWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTRERSPSPSF
                     RWPFSGKTSPPCSPANLSRHKAAAYDISEDEED"
     exon            240..339
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            340..456
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            457..608
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            609..687
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            688..830
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1019
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..5546
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5521..5526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /note="hexamer: AATAAA"
     polyA_site      5546
                     /gene="PCYT1A"
                     /gene_synonym="CCTA; CCTalpha; CGL5; CT; CTA; CTPCT;
                     PCYT1; SMDCRD"
                     /note="major polyA site"
ORIGIN      
        1 atcagctgcc ttccccgggc gtctccccgc aacctcctca acttccctag tcagtgacgc
       61 ggcgccggcc agaaatccga ccggaccggg ctcgggggag cgtgagttgc agttaaaaga
      121 agatggatgc acagtgttca gccaaggtca atgcaaggaa gaggagaaaa gaggcgcccg
      181 gacccaacgg ggcaacagaa gaagatgggg ttccttccaa agtgcagcgc tgtgcagtgg
      241 gcttacggca accagctcct ttttctgatg aaattgaagt tgactttagt aagccctatg
      301 tcagggtaac tatggaagaa gccagcagag gaactccttg tgagcgacct gtgagagttt
      361 atgccgatgg aatatttgac ttatttcact ctggtcacgc ccgagctctg atgcaagcga
      421 agaacctttt ccctaatacg tacctcattg tgggagtttg cagtgatgag ctcacacaca
      481 acttcaaagg cttcacggtg atgaacgaga atgagcgcta tgacgcagtc cagcactgcc
      541 gctacgtgga tgaggtggtg aggaatgcgc cctggacgct gacacccgag ttcctggccg
      601 aacaccggat tgattttgta gcccatgatg atattcctta ttcatctgct ggcagtgatg
      661 atgtttataa gcacatcaag gaggcaggca tgtttgctcc aacacagagg acagaaggta
      721 tctccacatc agacatcatc acccgaattg tgcgggatta tgatgtgtat gcgaggcgga
      781 acctgcagag gggctacaca gcaaaggagc tcaatgtcag ctttatcaac gagaagaaat
      841 accacttgca ggagagggtt gacaaagtaa agaagaaagt gaaagatgtg gaggaaaagt
      901 caaaagaatt tgttcagaag gtggaggaaa aaagcattga cctcattcag aagtgggagg
      961 agaagtcccg agaattcatt ggaagttttc tggaaatgtt tggtccggaa ggagcactga
     1021 aacatatgct gaaagagggg aagggccgga tgctgcaggc catcagcccg aagcagagcc
     1081 ccagcagcag ccctactcgc gagcgctccc cctccccctc tttccgatgg cccttctccg
     1141 gcaagacttc cccaccttgc tccccagcaa atctctccag gcacaaggct gcagcctatg
     1201 atatcagtga ggatgaagaa gactaatgtt tcctccctcc tttcctgtcc tccctttctg
     1261 tcccattacc ttcagaagct ctctgttgaa ttccaaattg tgaccccaac actaaaccta
     1321 aggacagcta caaaggaaag acaactgggg aaagaagacc taggactggg ggaacccaaa
     1381 cagcctgtcc tgcaagtgac atcactcacc cacacgaagg agggggactg caccttagaa
     1441 gcctgctctg catccattta ccccccccca agggctttgt ttcactgttt atgttcacgt
     1501 gatctcaaca gggaaattgt cattttcatt aattttttaa aaaattcgtc tttcaaatgt
     1561 agaactaatt ttttgcccct gaggagtggg cagaagaagg aggtagtgta atacgcagag
     1621 gccttttctt cccggtatac tatagcattt gccttccgtt ctgaagctat gtggcttgcc
     1681 aagtccttca cgaaatgagc cacgctgatg ggtaatttgt ggagaaatat cttaattttg
     1741 atttccttaa ggaaaagcga aaagcagagt tacagtcaca aatcccctga acgtactgct
     1801 gtcaccagtg tcattgtggc tgcgggaagg agatctgaac ctaacttttc ttactccctg
     1861 aggagtttct tgttggaccc aatggggtcc atgaagacac tgatggggat ggaagcagac
     1921 ccatggcatc aactgcagtg tttctgctgt gttcatcttc tcagaaaact ctgtgaccca
     1981 gttctttggg agcttttgtc agtctctttc gtgccatcta ccttcgaagg aaggaacact
     2041 tttagagtta caatgatgag gagaggagcg ggacaatact caatcctata ccaagtcgat
     2101 tgagattagt ttcctactgg gagtaagtgc ctggccctgc tactcctatt tgaatgctat
     2161 taatattacc tagcctttta caaaggatac tttgtaaatg tctgctgttg gaacttgatg
     2221 ttaatccatt ttggtcatag ccatattaga gagatttgca cagaatgtaa aggcaaaagc
     2281 ataggaaaca ccttcctttc taaagcaaca gccttatcac cttgacctct gtccctcttc
     2341 agaacattct tccctggaac cagtgctcca catctttctt ttcctctgag agctgcagct
     2401 ggcagggacc tccctctgct gctcctccag caagccacag agcataccct cacgtgacaa
     2461 gagtgtggta ggttttctcc ccacttttca cacacgcctg gtggttgtgg ttccatctgc
     2521 cttgttggct tgcccggggg gattcaacac ttgactttca aatcaaagaa tgctaatgct
     2581 tagcacttgc tgttgagcat gctctaactt ttaaagccct ggcctcctgt ctccttagct
     2641 gcttagctac gcggcctagc agatcatcac ccatcagtaa tcctgtggct agatccaggg
     2701 gaaaattgct tatgtccagg ccccacccct gtcactcata aatacccctt gtgggggtgg
     2761 aattttagtg ttttcagcta agaaaaccac attttctctt tccttgcctc tgctaggact
     2821 gtgagcatct catagtccac tccctaaaca ctttctggta acacctagtt ttccgcctga
     2881 gggtcagagg ctagaatcat tgcctgcaca gcaggactac cttggttctt aaaacttgga
     2941 tcacccaggc aaggcgtggt ggctcacgcc tgtcatctca gcactttggg agactgaggc
     3001 tggtggatca cttgaggcca cgagtttgag accagcctga ccaacgtggt gaaaccccat
     3061 ctctactaaa aatacaaaaa ttagccaggc gtggtggtgg gcacctgtaa tcccagctac
     3121 ttgggaggct gaggcaggag aatcccttga acccaggagg cggaggttgc agtgagcaga
     3181 gatggtgccg ctgcagtcca gcctgggtga cagagcgaga ctctgtctcc aaaacaaaac
     3241 aaaaaccaca cacacataca gaaataaaga gaaataatca actagaaaaa aaacaaaact
     3301 tggatcaccc agaaagagcg tcacagaggc gagggagaat gaagcctctt gttatcctga
     3361 tcgcctatga caacgacatt tctaactctt ctttgaagcg cagttcttac tcttaaatct
     3421 ggtcatacaa gctttggctt ggaatcctgt ctgtctttga aacagctgaa gttagtgttt
     3481 tcctgtgtta cccagtgtta catagtctca tctttgaact tttctgcgtc agccttctct
     3541 ctttgggaat gctgcctgtt tctgtctaac ttattctccc tggtgtggtg tggcagtgaa
     3601 gtctgtgctg aagcccatct gcttggttcc tccaagtgca cattatgcaa gtgtatttgg
     3661 atagacaggg agatgcccgg aacgtggttt tcctcctccc tccctattgg agccatacct
     3721 cctaaatata ttgtctgggt cccaaatgtt tgagccctct tcctctgctc tcagtgggct
     3781 gctcttggca tgccctgtgc ttggaacggg acctgcaggc tgacaggcct ctgagactgc
     3841 tagggccagc aaacgtacat gttattggga aactcccttt ccccttttgg tgagcttcta
     3901 ccacctcagt ctaatcgttt tgactgaaag ctatttgtct gactgaaagc taaaatcttt
     3961 tatagtgtgg cctgttagat actttggggt aaatgtggtc tgaggtgctg gactagcacg
     4021 ggtagatgga gaaagctgtt gggaagttgc ataatgtggt catctgtgcc taattaaatg
     4081 aggttagagg tgtgcacacc accacaatgc ctgggatcta aatcttcaga tattaacgtt
     4141 tgaggctatg gagctgtttc cctgaatcac acacgagaaa gcagtcaaca tgggtgggcc
     4201 aaatgtggcc aagatgagag agccagaagt gttggcctta gagcagagcc tctgtgtgtg
     4261 agacattaga aggccagagc cggggaggtg ccggcgcagc cactgccttc cggtgggccg
     4321 gacggaaagc ttggtcttat gctagattca gaagcactga agaatggagg ttgacacttt
     4381 agaggactag gaagggatca tttgtgctct tgcagctatt gctgatagct agagccaaag
     4441 accattatat tgctgaggct tgggtaagaa agttagggat gtgaggaggt tgaaaatcct
     4501 gaagaagaga aaaaatatga gggaggctaa ggaaacttag ccactttctg atcactgatg
     4561 actccttagg ctgcacctga gtgatggtgg tgtttttgga ggctgtacct gagtgatggt
     4621 ggtttttggg gggctgcacc tgagcgatag tgattttttt ttggaggctg cacagctggt
     4681 cagaccatct ctagatgttc tttccacagt ggttcatagg gcgtcattcc cccttcctgg
     4741 attattttga ctatgggtag cttattttca agagctcttt gcctttttaa tcctgttcca
     4801 gatcacttat ccaaaatctt tccaaatcaa ttatattttg ctgcttttct ttaattccct
     4861 aaaagccata gcttctagat tttctatcag cacacattct caccagaagt tgaaccaata
     4921 caggtagaaa aagaaagccc ccttaatgtt tttaactgat gatatttttt taagcttacc
     4981 aatataagta tttttaaagg ttctattttt caaagtcata acaatgattg ttcttgtttt
     5041 ctctcataga atagactgcc atcggataaa gagtggtccc tagcttctat ttttccaagt
     5101 aaataagtag aacatgttct tgggattata ccattaaatg ttaattttct tgaagaagaa
     5161 agattgttgt ctgccaagat tttatgttag cgctcggatt gaggcagaaa acggaagcac
     5221 caggtttaac actgggatga cttgggttgt gttcctggag gtttgaagtg ggccttcccg
     5281 ccttttgagg gggaaaactg actgttttga acacatatcc tggattagtt gggttgtttt
     5341 gtattgagga tccctaaaag acaaaagagt cttggaaagg actgctggct tcgtcttgaa
     5401 cgttcgcccc atcaccagtg tgagtccgtg gggaagtctt cctcaagtgg ggttcagtct
     5461 gtaattttcc tgtctgttct tagtttgttc tgagttattt tggattttgt tctttagcca
     5521 aataaaggca gtgtggtttt acctga
//
