LOCUS       NM_016297               5339 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens prenylcysteine oxidase 1 (PCYOX1), mRNA.
ACCESSION   NM_016297
VERSION     NM_016297.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Banfi,C., Amadio,P., Zara,M., Brioschi,M., Sandrini,L. and
            Barbieri,S.S.
  TITLE     Prenylcysteine Oxidase 1 (PCYOX1), a New Player in Thrombosis
  JOURNAL   Int J Mol Sci 23 (5), 2831 (2022)
   PUBMED   35269975
  REMARK    GeneRIF: Prenylcysteine Oxidase 1 (PCYOX1), a New Player in
            Thrombosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Banfi,C., Baetta,R., Barbieri,S.S., Brioschi,M., Guarino,A.,
            Ghilardi,S., Sandrini,L., Eligini,S., Polvani,G., Bergman,O.,
            Eriksson,P. and Tremoli,E.
  TITLE     Prenylcysteine oxidase 1, an emerging player in atherosclerosis
  JOURNAL   Commun Biol 4 (1), 1109 (2021)
   PUBMED   34548610
  REMARK    GeneRIF: Prenylcysteine oxidase 1, an emerging player in
            atherosclerosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5339)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5339)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5339)
  AUTHORS   Herrera-Marcos,L.V., Lou-Bonafonte,J.M., Martinez-Gracia,M.V.,
            Arnal,C., Navarro,M.A. and Osada,J.
  TITLE     Prenylcysteine oxidase 1, a pro-oxidant enzyme of low density
            lipoproteins
  JOURNAL   Front Biosci (Landmark Ed) 23 (6), 1020-1037 (2018)
   PUBMED   28930587
  REMARK    GeneRIF: Elevated prenylcysteine oxidase 1 (PCYOX1) activity could
            help to propagate the oxidative burden of low density lipoproteins
            (LDLs), thus making PCYOX1 a potential pharmacological target and a
            new biomarker in cardiovascular disease [Review].
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5339)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 5339)
  AUTHORS   Digits,J.A., Pyun,H.J., Coates,R.M. and Casey,P.J.
  TITLE     Stereospecificity and kinetic mechanism of human prenylcysteine
            lyase, an unusual thioether oxidase
  JOURNAL   J Biol Chem 277 (43), 41086-41093 (2002)
   PUBMED   12186880
  REMARK    GeneRIF: initial studies of the kinetic mechanism and
            stereospecificity of this unusual enzyme
REFERENCE   8  (bases 1 to 5339)
  AUTHORS   Tschantz,W.R., Digits,J.A., Pyun,H.J., Coates,R.M. and Casey,P.J.
  TITLE     Lysosomal prenylcysteine lyase is a FAD-dependent thioether oxidase
  JOURNAL   J Biol Chem 276 (4), 2321-2324 (2001)
   PUBMED   11078725
REFERENCE   9  (bases 1 to 5339)
  AUTHORS   Tschantz,W.R., Zhang,L. and Casey,P.J.
  TITLE     Cloning, expression, and cellular localization of a human
            prenylcysteine lyase
  JOURNAL   J Biol Chem 274 (50), 35802-35808 (1999)
   PUBMED   10585463
REFERENCE   10 (bases 1 to 5339)
  AUTHORS   Zhang,L., Tschantz,W.R. and Casey,P.J.
  TITLE     Isolation and characterization of a prenylcysteine lyase from
            bovine brain
  JOURNAL   J Biol Chem 272 (37), 23354-23359 (1997)
   PUBMED   9287348
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC417137.1, AY359063.1 and AC079338.5.
            
            On Nov 22, 2018 this sequence version replaced NM_016297.3.
            
            Summary: Prenylcysteine is released during the degradation of
            prenylated proteins. PCYOX1 catalyzes the degradation of
            prenylcysteine to yield free cysteines and a hydrophobic isoprenoid
            product (Tschantz et al., 1999 [PubMed 10585463]).[supplied by
            OMIM, Mar 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.246017.1,
                                           SRR1660805.179835.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000433351.7/ ENSP00000387654.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DC417137.1         39-47
            10-1690             AY359063.1         1-1681
            1691-5339           AC079338.5         17889-21537
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.3"
     gene            1..5339
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /note="prenylcysteine oxidase 1"
                     /db_xref="GeneID:51449"
                     /db_xref="HGNC:HGNC:20588"
                     /db_xref="MIM:610995"
     exon            1..140
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1546
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /EC_number="1.8.3.5"
                     /note="prenylcysteine lyase"
                     /codon_start=1
                     /product="prenylcysteine oxidase 1 precursor"
                     /protein_id="NP_057381.3"
                     /db_xref="CCDS:CCDS1902.1"
                     /db_xref="GeneID:51449"
                     /db_xref="HGNC:HGNC:20588"
                     /db_xref="MIM:610995"
                     /translation="MGRVVAELVSSLLGLWLLLCSCGCPEGAELRAPPDKIAIIGAGI
                     GGTSAAYYLRQKFGKDVKIDLFEREEVGGRLATMMVQGQEYEAGGSVIHPLNLHMKRF
                     VKDLGLSAVQASGGLLGIYNGETLVFEESNWFIINVIKLVWRYGFQSLRMHMWVEDVL
                     DKFMRIYRYQSHDYAFSSVEKLLHALGGDDFLGMLNRTLLETLQKAGFSEKFLNEMIA
                     PVMRVNYGQSTDINAFVGAVSLSCSDSGLWAVEGGNKLVCSGLLQASKSNLISGSVMY
                     IEEKTKTKYTGNPTKMYEVVYQIGTETRSDFYDIVLVATPLNRKMSNITFLNFDPPIE
                     EFHQYYQHIVTTLVKGELNTSIFSSRPIDKFGLNTVLTTDNSDLFINSIGIVPSVREK
                     EDPEPSTDGTYVWKIFSQETLTKAQILKLFLSYDYAVKKPWLAYPHYKPPEKCPSIIL
                     HDRLYYLNGIECAASAMEMSAIAAHNAALLAYHRWNGHTDMIDQDGLYEKLKTEL"
     exon            141..347
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            348..522
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            523..734
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            735..887
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            888..5339
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3425..3430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /note="hexamer: ATTAAA"
     polyA_site      3447
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
     regulatory      5320..5325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /note="hexamer: AATAAA"
     polyA_site      5339
                     /gene="PCYOX1"
                     /gene_synonym="PCL1"
                     /note="major polyA site"
ORIGIN      
        1 gaggccagct gcagagcttg tggaggccat ggggcgcgtc gtcgcggagc tcgtctcctc
       61 gctgctgggg ttgtggctgt tgctgtgcag ctgcggatgc cccgagggcg ccgagctgcg
      121 tgctccgcca gataaaatcg cgattattgg agccggaatt ggtggcactt cagcagccta
      181 ttacctgcgg cagaaatttg ggaaagatgt gaagatagac ctgtttgaaa gagaagaggt
      241 cgggggccgc ctggctacca tgatggtgca ggggcaagaa tacgaggcag gaggttctgt
      301 catccatcct ttaaatctgc acatgaaacg ttttgtcaaa gacctgggtc tctctgctgt
      361 tcaggcctct ggtggcctac tggggatata taatggagag actctggtat ttgaggagag
      421 caactggttc ataattaacg tgattaaatt agtttggcgc tatggatttc aatccctccg
      481 tatgcacatg tgggtagagg acgtgttaga caagttcatg aggatctacc gctaccagtc
      541 tcatgactat gccttcagta gtgtcgaaaa attacttcat gctctaggag gagatgactt
      601 ccttggaatg cttaatcgaa cacttcttga aaccttgcaa aaggccggct tttctgagaa
      661 gttcctcaat gaaatgattg ctcctgttat gagggtcaat tatggccaaa gcacggacat
      721 caatgccttt gtgggggcgg tgtcactgtc ctgttctgat tctggccttt gggcagtaga
      781 aggtggcaat aaacttgttt gctcagggct tctgcaggca tccaaaagca atcttatatc
      841 tggctcagta atgtacatcg aggagaaaac aaagaccaag tacacaggaa atccaacaaa
      901 gatgtatgaa gtggtctacc aaattggaac tgagactcgt tcagacttct atgacatcgt
      961 cttggtggcc actccgttga atcgaaaaat gtcgaatatt acttttctca actttgatcc
     1021 tccaattgag gaattccatc aatattatca acatatagtg acaactttag ttaaggggga
     1081 attgaataca tctatcttta gctctagacc catagataaa tttggcctta atacagtttt
     1141 aaccactgat aattcagatt tgttcattaa cagtattggg attgtgccct ctgtgagaga
     1201 aaaggaagat cctgagccat caacagatgg aacatatgtt tggaagatct tttcccaaga
     1261 aactcttact aaagcacaaa ttttaaagct ctttctgtcc tatgattatg ctgtgaagaa
     1321 gccatggctt gcatatcctc actataagcc cccggagaaa tgcccctcta tcattctcca
     1381 tgatcgactt tattacctca atggcataga gtgtgcagca agtgccatgg agatgagtgc
     1441 cattgcagcc cacaacgctg cactccttgc ctatcaccgc tggaacgggc acacagacat
     1501 gattgatcag gatggcttat atgagaaact taaaactgaa ctatgaagtg acacactcct
     1561 ttttcccctc ctagttccaa atgactatca gtggcaaaaa agaacaaaat ctgagcagag
     1621 atgattttga accagatatt ttgccattat cattgtttaa taaaagtaat ccctgctggt
     1681 cataggaaaa cacacggttc taattaagtg tgaaggtata gctattgcac ttatgccatc
     1741 tccaaaattt cttaagtatt ctttcactat ccattaggag tttttcttaa acttgtctga
     1801 taataagaat cacctggagt taggaggtgg tggttgcagt gagccaatct caccattgca
     1861 cttcagcctg agcaacacga gcaaaactcc gtctcaaaag taaataaaaa taatcacctg
     1921 gagtttgtta aaccatatgg attctcaagc tcctctcttg aagattctga ttcagtaggt
     1981 ctgggagtgg cgcgctggat tttgatcaaa attgtagagc attttaaggt gagtacctga
     2041 gggagaactt aaagacatct tagttgggga gtagtccttt tgaattttac agctagatat
     2101 aatcttcagt cagataaaat ttatgggagc tggtgtctta tgcctgactc ttagtaattt
     2161 cataccggtt tgaagtacgt gtgcccatgc ctaaagcctt gactttcaga atgttgtctt
     2221 ttgattcttc tgtcttgatt tgattagggg tgaaatttag aagtcttagt aatgtaactt
     2281 gaagatgtta aacaaaaatc tcaagtaaaa tgaaaagcaa atatgggcta ctgaattaag
     2341 aaactggcat tctagtatta aatcctcact tcaggagctt ttaaaaatac tgagaccccc
     2401 ccataaccag agattcagat tcaaagactg aggataggac cttagcattg tagctattta
     2461 aagtttctaa tgtgcaccca gggttgggaa tcaccaatgt gggtgtgaaa atgcctacaa
     2521 agggttttag tgccttagaa gtcctaagaa gcccaatctg tatcaaagca gatccatttt
     2581 gcaaggatct ttcttttaga actttctcag ttctcttagt aagaacttta gaagtaatct
     2641 tgataataag cacagacagc ctaacagcag aggcaactta aataactcct gagcagttgg
     2701 cactagaaca gaatacttgg aatgacacca aagttaacca agtccagcat atgtccaaag
     2761 agttaagtgt ttcatttact gtagcattct gggtgagaaa ttggttgctg aaatcttaag
     2821 acagtggtct caaccttggc tgcacattgg aatcacctgt agggttttaa agcatccaaa
     2881 tggtaattaa caggcagcaa aacttcagaa ctagttctgc atctactgtg caaagatcat
     2941 gattaactgt caagacactg gtagaacaga acaagcaaaa gattaagagt tcaaaagtaa
     3001 atgcaaccaa tttaacatgt agtgttatta aaaaattaca aaggcctaga ccagcctggg
     3061 caacatggtg aaaccccatc tctacaaaaa atttttaaaa agttggccag gcatggtaat
     3121 gcgcgcctgt ggtcccagct gctcgggagg ctgaggtggg aggatcactt gagccttgga
     3181 ggtcaaggct gcagtgaatc atgatcatgc cactgtactc cagcctgggc aacagagacc
     3241 atcttaaaaa aaaaaaatcc ttccattgaa tagaaacttc aaagtccttc atcagatcat
     3301 ggcattagct actttagcaa aatagtgtaa actttggttc tgagtaaaaa tgacttcttc
     3361 cctaccacag ttgtgaatgt taatatgctg ataaaactgc caagatatat ttgataggta
     3421 ggggattaaa ctcatggttt gtcaaaagag tgtttttttc tagttttatt cttaacagat
     3481 atgttgaggt attcatattt gtttcctttt gtggttttaa tgaagacaat ttgtaaagta
     3541 atactgttat gtatatgcta aatgttggta aatactaatt aatttccatc atttgtagac
     3601 ttgttttgca atgggatata tttttactta taataccaat ttaggctggg cgcagtggct
     3661 cacgcctgta atcccagcac tttgggaggc caaggcaaac ggatcacgag gtcaggagat
     3721 caagaccatc ctggccaaca tggtgaaacc ccatctctac tgaatacaca aaaattggct
     3781 ggacatggtg gcgcatgtct gtaatcccag ctactgtaat ctcagctact tgggaggctg
     3841 aggcaggaga attgcctcaa ccgggaggcg gaggttgcag tgagccaaga tcgcaccact
     3901 gcactccagc ctggcaacag agcgagactc tgtctcagaa aaaaaaaaca aaaaccagtt
     3961 taggctgggt atggtggctc accagcactt tgggaggctg aggcaggtgg atctcttgag
     4021 gtcgggagtt tgagaccagc ctggccaaca tggtgaaacc ctgtctttac taaaaataca
     4081 aaaattagcc aggcatggtg gcgtgtgcca gtaatcccag ctacttggga ggctgaggcg
     4141 cgagaatcgc ttgaaccctg gagatggagg ttgcagtgag cagagatcgc accactgcac
     4201 tccagcctgg gcaagagtga gactgtgtct caaaaggaaa aaaaaaaaat taaaaatgtt
     4261 gctggtatta catcaaacct actctggata aattttccag tagggcatct gaaattgctt
     4321 tggcaaaagc aaaacaggtt atcaatacac caaggacatc aagtagtttt aatgcaacta
     4381 cagtgttgag tgctttttta ctgggagaaa aatcctagca gaacctgcta ctctgggcgc
     4441 attaggtagt tttatgcaac tatagtatta agagttttat tggaagaaaa aacactgtca
     4501 gagcttactg ctccagacct ttttttttaa tagaagagac tggtaaaaat gatgcagggg
     4561 ctgaatgaaa gccagctctg ctccagtggc tctctgagtt gagcatgcat cagttacctt
     4621 aagggcttgt taaaacatcg ctaggctcca ccctgtttat tcaataagtt tgttatggga
     4681 tataagaaga tgcattttat taagttcccc agtgatgctg ctgttctact ataggaacca
     4741 cacttgaaga actagttcta caattcagct gtatgttgta aaggagtcaa gtcaatgaag
     4801 gagttataaa gagttgctga aaaatggagt aacagtgtat gggacttcac atcagttctc
     4861 cttattgatt gttagtttga tccctcttgt tcttttgttg taaacatttt ctataattag
     4921 gaaatgccat ttaagagtga gagaggtata tatctatgag ccattgtgtt tggtgtttta
     4981 caagaacttt accatactgg tgtgtagtcc attctgtaca gtttaaaagt gattcacgat
     5041 ttgcaggctt tttatcagat cacaaaaaaa tcagtcttta agcatttgct tggtaaggtt
     5101 tcttaagatt aggtttataa tacaaccatc tgtaatgtat ctctcgtttg agcttgtggg
     5161 ccatacaatt cattaactag atgaatacat tgtggacagc atcctcacta cccctctcta
     5221 ctcactcaca aagaaccatg atacactgga atgtttttct ctggaatcct ctttctactc
     5281 ttgtattaaa atttttcccc acttcatgat tatattcaaa ataaacaact acatcatta
//
