LOCUS       NM_000439               5136 bp    mRNA    linear   PRI 07-MAY-2024
DEFINITION  Homo sapiens proprotein convertase subtilisin/kexin type 1 (PCSK1),
            transcript variant 1, mRNA.
ACCESSION   NM_000439
VERSION     NM_000439.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Manco,L., Albuquerque,D., Aranda,B., Rodrigues,D.,
            Machado-Rodrigues,A.M. and Padez,C.
  TITLE     Differential sex-association between PCSK1 polymorphisms and
            obesity risk in Portuguese children
  JOURNAL   Am J Hum Biol 36 (5), e24023 (2024)
   PUBMED   38009939
  REMARK    GeneRIF: Differential sex-association between PCSK1 polymorphisms
            and obesity risk in Portuguese children.
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Guijo,B., Argente,J. and Martos-Moreno,G.A.
  TITLE     The N221D variant in PCSK1 is highly prevalent in childhood obesity
            and can influence the metabolic profile
  JOURNAL   J Pediatr Endocrinol Metab 36 (12), 1140-1145 (2023)
   PUBMED   37877373
  REMARK    GeneRIF: The N221D variant in PCSK1 is highly prevalent in
            childhood obesity and can influence the metabolic profile.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5136)
  AUTHORS   Velazquez-Roman,J., Angulo-Zamudio,U.A., Leon-Sicairos,N.,
            Flores-Villasenor,H., Benitez-Baez,M., Espinoza-Salomon,A.,
            Karam-Leon,A., Villamil-Ramirez,H., Canizales-Quinteros,S.,
            Macias-Kauffer,L., Monroy-Higuera,J., Acosta-Smith,E. and
            Canizalez-Roman,A.
  TITLE     Association of PCSK1 and PPARG1 Allelic Variants with Obesity and
            Metabolic Syndrome in Mexican Adults
  JOURNAL   Genes (Basel) 14 (9), 1775 (2023)
   PUBMED   37761915
  REMARK    GeneRIF: Association of PCSK1 and PPARG1 Allelic Variants with
            Obesity and Metabolic Syndrome in Mexican Adults.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5136)
  AUTHORS   Shah,B.P., Sleiman,P.M., Mc Donald,J., Moeller,I.H. and Kleyn,P.
  TITLE     Functional characterization of all missense variants in LEPR,
            PCSK1, and POMC genes arising from single-nucleotide variants
  JOURNAL   Expert Rev Endocrinol Metab 18 (2), 209-219 (2023)
   PUBMED   36864747
  REMARK    GeneRIF: Functional characterization of all missense variants in
            LEPR, PCSK1, and POMC genes arising from single-nucleotide
            variants.
REFERENCE   5  (bases 1 to 5136)
  AUTHORS   Folon,L., Baron,M., Toussaint,B., Vaillant,E., Boissel,M.,
            Scherrer,V., Loiselle,H., Leloire,A., Badreddine,A., Balkau,B.,
            Charpentier,G., Franc,S., Marre,M., Aboulouard,S., Salzet,M.,
            Canouil,M., Derhourhi,M., Froguel,P. and Bonnefond,A.
  TITLE     Contribution of heterozygous PCSK1 variants to obesity and
            implications for precision medicine: a case-control study
  JOURNAL   Lancet Diabetes Endocrinol 11 (3), 182-190 (2023)
   PUBMED   36822744
  REMARK    GeneRIF: Contribution of heterozygous PCSK1 variants to obesity and
            implications for precision medicine: a case-control study.
REFERENCE   6  (bases 1 to 5136)
  AUTHORS   Benjannet,S., Reudelhuber,T., Mercure,C., Rondeau,N., Chretien,M.
            and Seidah,N.G.
  TITLE     Proprotein conversion is determined by a multiplicity of factors
            including convertase processing, substrate specificity, and
            intracellular environment. Cell type-specific processing of human
            prorenin by the convertase PC1
  JOURNAL   J Biol Chem 267 (16), 11417-11423 (1992)
   PUBMED   1597471
REFERENCE   7  (bases 1 to 5136)
  AUTHORS   Seidah,N.G., Hamelin,J., Gaspar,A.M., Day,R. and Chretien,M.
  TITLE     The cDNA sequence of the human pro-hormone and pro-protein
            convertase PC1
  JOURNAL   DNA Cell Biol 11 (4), 283-289 (1992)
   PUBMED   1605851
REFERENCE   8  (bases 1 to 5136)
  AUTHORS   Creemers,J.W., Roebroek,A.J. and Van de Ven,W.J.
  TITLE     Expression in human lung tumor cells of the proprotein processing
            enzyme PC1/PC3. Cloning and primary sequence of a 5 kb cDNA
  JOURNAL   FEBS Lett 300 (1), 82-88 (1992)
   PUBMED   1547893
REFERENCE   9  (bases 1 to 5136)
  AUTHORS   Seidah,N.G., Mattei,M.G., Gaspar,L., Benjannet,S., Mbikay,M. and
            Chretien,M.
  TITLE     Chromosomal assignments of the genes for neuroendocrine convertase
            PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2)
            to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region)
  JOURNAL   Genomics 11 (1), 103-107 (1991)
   PUBMED   1765368
REFERENCE   10 (bases 1 to 5136)
  AUTHORS   Sanke,T., Bell,G.I., Sample,C., Rubenstein,A.H. and Steiner,D.F.
  TITLE     An islet amyloid peptide is derived from an 89-amino acid precursor
            by proteolytic processing
  JOURNAL   J Biol Chem 263 (33), 17243-17246 (1988)
   PUBMED   3053705
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA192592.1, X64810.1 and
            AC008951.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000439.4.
            
            Summary: This gene encodes a member of the subtilisin-like
            proprotein convertase family, which includes proteases that process
            protein and peptide precursors trafficking through regulated or
            constitutive branches of the secretory pathway. The encoded protein
            undergoes an initial autocatalytic processing event in the ER to
            generate a heterodimer which exits the ER and sorts to subcellular
            compartments where a second autocatalytic even takes place and the
            catalytic activity is acquired. The protease is packaged into and
            activated in dense core secretory granules and expressed in the
            neuroendocrine system and brain. This gene encodes one of the seven
            basic amino acid-specific members which cleave their substrates at
            single or paired basic residues. It functions in the proteolytic
            activation of polypeptide hormones and neuropeptides precursors.
            Mutations in this gene have been associated with susceptibility to
            obesity and proprotein convertase 1/3 deficiency. Alternatively
            spliced transcript variants encoding multiple isoforms have been
            observed for this gene [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.27882.1,
                                           SRR1660803.86657.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311106.8/ ENSP00000308024.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DA192592.1         34-50
            18-1274             X64810.1           1-1257
            1275-1275           AC008951.6         4279-4279
            1276-1855           X64810.1           1259-1838
            1856-1856           AC008951.6         17671-17671
            1857-5054           X64810.1           1840-5037
            5055-5136           AC008951.6         24665-24746
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q15"
     gene            1..5136
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="proprotein convertase subtilisin/kexin type 1"
                     /db_xref="GeneID:5122"
                     /db_xref="HGNC:HGNC:8743"
                     /db_xref="MIM:162150"
     exon            1..386
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..2468
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /EC_number="3.4.21.93"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; prohormone convertase 3; prohormone convertase
                     1; neuroendocrine convertase 1; proprotein convertase 1/3"
                     /codon_start=1
                     /product="neuroendocrine convertase 1 isoform 1
                     preproprotein"
                     /protein_id="NP_000430.3"
                     /db_xref="CCDS:CCDS4081.1"
                     /db_xref="GeneID:5122"
                     /db_xref="HGNC:HGNC:8743"
                     /db_xref="MIM:162150"
                     /translation="MERRAWSLQCTAFVLFCAWCALNSAKAKRQFVNEWAAEIPGGPE
                     AASAIAEELGYDLLGQIGSLENHYLFKHKNHPRRSRRSAFHITKRLSDDDRVIWAEQQ
                     YEKERSKRSALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKG
                     VVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAM
                     QANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKT
                     VEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSA
                     SQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF
                     ALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALV
                     DLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQ
                     FEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGT
                     WTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMV
                     DPGEEQPTQENPKENTLVSKSPSSSSVGGRRDELEEGAPSQAMLRLLQSAFSKNSPPK
                     QSPKKSPSAKLNIPYENFYEALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHRDDR
                     LLQALVDILNEEN"
     exon            387..491
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            492..602
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            603..749
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            750..826
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            827..915
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1088
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1301
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1402
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1636
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1794
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1928
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2090
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..5136
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2469..2474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="hexamer: AATAAG"
     polyA_site      2487
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="major polyA site"
     regulatory      4967..4972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="hexamer: AATACA"
     polyA_site      4990
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="major polyA site"
     regulatory      5107..5112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
                     /note="hexamer: AAGAAA"
     polyA_site      5136
                     /gene="PCSK1"
                     /gene_synonym="BMIQ12; NEC1; PC1; PC1/3; PC3; SPC3"
ORIGIN      
        1 actcagcctg gagaccgaag cgcttcactg agcgctcgcc gccgcccagc ctctcctctc
       61 gcgcctccta gctcttcgca gagcaaccag gagccaggag tggtctagag cccgagggtg
      121 ggaaggggga gtctgtctgg cttttctcct atcttgcttc tttttcctct tcccttccca
      181 ctcttgttca agcgagtgtg tgagctatgg agcgaagagc ctggagtctg cagtgcactg
      241 ctttcgtcct cttttgcgct tggtgtgcac tgaacagtgc aaaagcgaaa aggcaatttg
      301 tcaatgaatg ggcagcggag atccccgggg gcccggaagc agcctcggcc atcgccgagg
      361 agctgggcta tgaccttttg ggtcagattg gttcacttga aaatcactac ttattcaaac
      421 ataaaaacca ccccagaagg tctcgaagga gtgcctttca tatcactaag agattatctg
      481 atgatgatcg tgtgatatgg gctgaacaac agtatgaaaa agaaagaagt aaacgttcag
      541 ctctaaggga ctcagcacta aatctcttca atgatcccat gtggaatcag caatggtact
      601 tgcaagatac caggatgacg gcagccctgc ccaagctgga ccttcatgtg atacctgttt
      661 ggcaaaaagg cattacgggc aaaggagttg ttatcaccgt actggatgat ggtttggagt
      721 ggaatcacac ggacatttat gccaactatg atccagaggc tagctatgat tttaatgata
      781 atgaccatga tccatttccc cgatatgatc ccacaaacga gaacaaacac gggaccagat
      841 gtgcaggaga aattgccatg caagcaaata atcacaaatg cggggttgga gttgcataca
      901 attccaaagt tggaggcata agaatgctgg atggcattgt gacggatgct attgaggcca
      961 gttcaattgg attcaatcct ggacacgtgg atatttacag tgcaagctgg ggccctaatg
     1021 atgatgggaa aactgtggag gggcctggcc ggctagccca gaaggctttt gaatatggtg
     1081 tcaaacaggg gagacagggg aaggggtcca tcttcgtctg ggcttcggga aacggggggc
     1141 gtcagggaga taattgtgac tgtgatggct acacagacag catctacacc atctccatca
     1201 gcagtgcctc ccagcaaggc ctatccccct ggtacgctga gaagtgctcc tccacactgg
     1261 ccacctctta cagcagcgga gattacaccg accagagaat cacgagcgct gacctgcaca
     1321 atgactgcac ggagacgcac acaggcacct cggcctctgc acctctggct gctggcatct
     1381 tcgctctggc cctggaagca aacccaaatc tcacctggcg agatatgcag cacctggttg
     1441 tctggacctc tgagtatgac ccgctggcca ataaccctgg atggaaaaag aatggagcag
     1501 gcttgatggt gaatagtcga tttggatttg gcttgctaaa tgccaaagct ctggtggatt
     1561 tagctgaccc caggacctgg aggagcgtgc ctgagaagaa agagtgtgtt gtaaaggaca
     1621 atgactttga gcccagagcc ctgaaagcta atggagaagt tatcattgaa attccaacaa
     1681 gagcttgtga aggacaagaa aatgctatca agtccctgga gcatgtacaa tttgaagcaa
     1741 caattgaata ttcccgaaga ggagaccttc atgtcacact tacttctgct gctggaacta
     1801 gcactgtgct cttggctgaa agagaacggg atacatctcc taatggcttt aagaattggg
     1861 acttcatgtc tgttcacaca tggggagaga accctatagg tacttggact ttgagaatta
     1921 cagacatgtc tggaagaatt caaaatgaag gaagaattgt gaactggaag ctgattttgc
     1981 acgggacctc ttctcagcca gagcatatga agcagcctcg tgtgtacacg tcctacaaca
     2041 ctgttcagaa tgacagaaga ggggtggaga agatggtgga tccaggggag gagcagccca
     2101 cacaagagaa ccctaaggag aacaccctgg tgtccaaaag ccccagcagc agcagcgtag
     2161 ggggccggag ggatgagttg gaggagggag ccccttccca ggccatgctg cgactcctgc
     2221 aaagtgcttt cagtaaaaac tcaccgccaa agcaatcacc aaagaagtcc ccaagtgcaa
     2281 agctcaacat cccttatgaa aacttctacg aagccctgga aaagctgaac aaaccttccc
     2341 agcttaaaga ctctgaagac agtctgtata atgactatgt tgatgttttt tataacacta
     2401 aaccttacaa gcacagagac gaccggctgc ttcaagctct ggtggacatt ctgaatgagg
     2461 aaaattaaaa taagtgtgtg gtcccaagtt ggaaatattc atgcttcttc cttaccctgc
     2521 gattttgcct gtgtctgaag tggttgtttt gtcatgaatt cttatgctta taatatcctt
     2581 tgtggcacct tttctttttc tccctaaact gtacatgtga aggggatgag ctcaagcagg
     2641 aagttcaact tccagaattg atcataggta tttcaaaaca catctttcct gtctgcacaa
     2701 gtgaagtgtt ttgttctttc tggagtcaca gttgacaaaa agctcttaca ctacattaga
     2761 acactgcatt agagcccatt tcaattctca aaagaaaagg caaaacctgg gatatcaatt
     2821 aatttgaaaa cataatctgc aaagaatgag aaggagtcag aaactgtttc tgtagcttgt
     2881 tccctgtctt gtccatgtgg ttcttcaaat tttgatgcca agaaagtatt tggtaggcct
     2941 aatgaaggag ttcactgtaa gactcattcc ctagatcttt ctattccaaa gtgccactca
     3001 ttcctgtagt caaaatctgg tcatgttggt caaaagctgg attatttaga tctagaaaca
     3061 gatcttgaaa tctgaatgct ctggtttgag caattttcga acattctttg cctggtgcac
     3121 tgtgtctgtg gtgccagagg cgtccgtgga tccagaggtg gttatgactc gtgctgcatg
     3181 cctggtcttt cctctgtttc tccttctgaa agttttctat acctgtctcc tttctcagcc
     3241 acaaaataaa tgttgggaga aatgatatat accactttcc cagaaaaaaa aaaacttaca
     3301 cttgggactt ggcaaattcc tagtcacaat ttttttcagc agtaacagga aaccacttat
     3361 cacatggaga cctaatgtaa taatagaaaa atactcataa tagggagaaa ccaagagaag
     3421 ttttgttttt gtttttttcc aactgtgttc attagaacag cgtgttctaa gtatttgaaa
     3481 ctgaatgttt attccttgat actaaaagtt cttctccaat cctatcactg atagtgtcca
     3541 aattctcacc aaattgctcc taagcttcaa atcagaagca gaaactggca ggccatggac
     3601 cttaattgtc cctcaggtag attttgtttg gtatgcagaa tgtttttaaa atatgagtgg
     3661 ttattgaaaa tatgatgttt cacataaaac ctcattctcg gacccatctt tgctcatggc
     3721 aacagttagc tggagctgag tagcagctgc ctgattagat gactctcagt ccccatggca
     3781 ccctgctcca tgttacctag agcaggcact tgattccttg ctgggcagta tccaataggc
     3841 atttgatttt gcccactcct acactaagcg aatgtgtaca aagtgtaaat gcattaggaa
     3901 aaacaaacta cccgcatctt ctgttaggca ggatctgtac aataataatt atgagtttgc
     3961 ttatgtaatc tcacctcacc tggatgatca ctaatactaa ttcatttatt actaaccttc
     4021 tggcttcctt ctctcaatat gcttacaaag tctccagtca cctacaatgc tggctttctc
     4081 ccactgagtt tgctgtttgc aatttttcca tgaagtttga acttcataag gtaattcatg
     4141 gcattgaact ggttcatgaa aagaacacta gagtctgtca tttgctttgg cttgaagtat
     4201 ggttggtaac acaaattttc acctgctctt ctaccatttg aatttgtgta gagggtgttt
     4261 gcagagcaat gcccgtaatg cttagagaat gttctcctaa aagacttgcg gaatcactct
     4321 gtccttggaa gtttcatata ttgtttgata tgaagtgtta gatagaattt ccaatattgg
     4381 agcatatcaa aaagtattaa aactaaaaag gaccagagaa ttcttagatt ggcccggaaa
     4441 ggccaataaa gagttagaat gaaaactcat tacttttcca ttcccaatct agtgctagat
     4501 gtataaatct ttcttttgat tcttcctaac aaaatatttt ctgggttaaa accccagcca
     4561 actcattggg ttgtagccaa aggttcactc tcaagaagct ttaatattta aataaaatca
     4621 tattgaatgt ttccaacctg gagtataata ttcagatata aaacagtttt gtcagtcttt
     4681 cttagtgcct gtgtggattt ttgtgaaaat gtcaaagaga aaacttatat actatttccc
     4741 ttgaaatttt aaactatatt ttctttacag gtatttataa tataccaatg cttttatcaa
     4801 acagaatttt aaagagcata ataaattata ttaaagaacc aaaagttttc ctgagaataa
     4861 gaaagtttca cccaataaaa tatttttgaa aggcatgttc ctctgtcaat gaaaaaaagt
     4921 acatgtatgt gttgtgatat taaaagtgac atttgtctaa tagcctaata caacatgtag
     4981 ctgagtttaa catgtgtggt cttggtattc ttaagggaac ttccacatta tacatttgat
     5041 gtattgacca gaatatgtaa aatatgctta taaatcagaa aaataaattg tttctcacta
     5101 agtcaaaaga aaaaaagctc atgtttccac cacaaa
//
