LOCUS       NM_020357               2285 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens PEST proteolytic signal containing nuclear protein
            (PCNP), transcript variant 1, mRNA.
ACCESSION   NM_020357
VERSION     NM_020357.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Chen,Y.G., Liu,H.X., Hong,Y., Dong,P.Z., Liu,S.Y., Gao,Y.R., Lu,D.,
            Li,T., Wang,D.Y., Wu,D.D. and Ji,X.Y.
  TITLE     PCNP is a novel regulator of proliferation, migration, and invasion
            in human thyroid cancer
  JOURNAL   Int J Biol Sci 18 (9), 3605-3620 (2022)
   PUBMED   35813472
  REMARK    GeneRIF: PCNP is a novel regulator of proliferation, migration, and
            invasion in human thyroid cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Han,Z.P., Yang,Y., Chen,H.Y., Zhong,M.Y. and Zhuang,G.H.
  TITLE     TIPE2 and PCNP expression abnormalities in peripheral blood
            mononuclear cells associated with disease activity in rheumatoid
            arthritis: a meta-analysis
  JOURNAL   Eur Rev Med Pharmacol Sci 25 (3), 1242-1249 (2021)
   PUBMED   33629294
  REMARK    GeneRIF: TIPE2 and PCNP expression abnormalities in peripheral
            blood mononuclear cells associated with disease activity in
            rheumatoid arthritis: a meta-analysis.
            Review article
REFERENCE   3  (bases 1 to 2285)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2285)
  AUTHORS   Dong,P., Fu,H., Chen,L., Zhang,S., Zhang,X., Li,H., Wu,D. and Ji,X.
  TITLE     PCNP promotes ovarian cancer progression by accelerating
            beta-catenin nuclear accumulation and triggering EMT transition
  JOURNAL   J Cell Mol Med 24 (14), 8221-8235 (2020)
   PUBMED   32548978
  REMARK    GeneRIF: PCNP promotes ovarian cancer progression by accelerating
            beta-catenin nuclear accumulation and triggering EMT transition.
REFERENCE   5  (bases 1 to 2285)
  AUTHORS   Wu,D.D., Gao,Y.R., Li,T., Wang,D.Y., Lu,D., Liu,S.Y., Hong,Y.,
            Ning,H.B., Liu,J.P., Shang,J., Shi,J.F., Wei,J.S. and Ji,X.Y.
  TITLE     PEST-containing nuclear protein mediates the proliferation,
            migration, and invasion of human neuroblastoma cells through MAPK
            and PI3K/AKT/mTOR signaling pathways
  JOURNAL   BMC Cancer 18 (1), 499 (2018)
   PUBMED   29716528
  REMARK    GeneRIF: PCNP mediates the proliferation, migration, and invasion
            of human neuroblastoma cells through mitogen-activated protein
            kinase and PI3K/AKT/mTOR signaling pathways, implying that PCNP is
            a therapeutic target for patients with neuroblastoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2285)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   7  (bases 1 to 2285)
  AUTHORS   Mori,T., Li,Y., Hata,H. and Kochi,H.
  TITLE     NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP,
            a PEST-containing nuclear protein
  JOURNAL   FEBS Lett 557 (1-3), 209-214 (2004)
   PUBMED   14741369
  REMARK    GeneRIF: NIRF has ubiquitination activity, the hallmark of a
            ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7
            cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo.
REFERENCE   8  (bases 1 to 2285)
  AUTHORS   Mori,T., Li,Y., Hata,H., Ono,K. and Kochi,H.
  TITLE     NIRF, a novel RING finger protein, is involved in cell-cycle
            regulation
  JOURNAL   Biochem Biophys Res Commun 296 (3), 530-536 (2002)
   PUBMED   12176013
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC073861.29.
            
            On May 17, 2019 this sequence version replaced NM_020357.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.176923.1,
                                           SRR1660803.167145.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265260.8/ ENSP00000265260.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC073861.29        115346-115445
            101-315             AC073861.29        120956-121170
            316-390             AC073861.29        126603-126677
            391-446             AC073861.29        131381-131436
            447-2285            AC073861.29        133793-135631
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q12.3"
     gene            1..2285
                     /gene="PCNP"
                     /note="PEST proteolytic signal containing nuclear protein"
                     /db_xref="GeneID:57092"
                     /db_xref="HGNC:HGNC:30023"
                     /db_xref="MIM:615210"
     exon            1..100
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..573
                     /gene="PCNP"
                     /note="isoform a is encoded by transcript variant 1;
                     PEST-containing nuclear protein"
                     /codon_start=1
                     /product="PEST proteolytic signal-containing nuclear
                     protein isoform a"
                     /protein_id="NP_065090.1"
                     /db_xref="CCDS:CCDS2942.1"
                     /db_xref="GeneID:57092"
                     /db_xref="HGNC:HGNC:30023"
                     /db_xref="MIM:615210"
                     /translation="MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGES
                     SSRSAEKRSAEEEAADLPTKPTKISKFGFAIGSQTTKKASAISIKLGSSKPKETVPTL
                     APKTLSVAAAFNEDEDSEPEEMPPEAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQK
                     LWERNIKSHLGNVHDQDN"
     exon            101..315
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            316..390
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            391..446
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            447..2285
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2238..2243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCNP"
                     /note="hexamer: AATAAA"
     polyA_site      2257
                     /gene="PCNP"
                     /note="major polyA site"
     polyA_site      2285
                     /gene="PCNP"
ORIGIN      
        1 cttggcgtgg ctgcagggga ggccgcggcg gggaaaatgg cggacgggaa ggcgggagac
       61 gagaagcctg aaaagtcgca gcgagctgga gccgccggag gacctgaaga agaagcagaa
      121 aaacctgtga aaactaagac tgtttcttcc agtaatggag gggaaagttc cagtcgcagc
      181 gctgagaagc gatcagctga agaagaagct gccgacctcc caacaaagcc tacaaagatc
      241 tccaagtttg gatttgccat aggtagtcag acgacaaaga aagcatcagc catatccatc
      301 aaacttggat caagtaagcc taaagaaact gttccaactc ttgctccaaa aactctttca
      361 gtagcagcag cttttaatga agatgaagat agtgaaccag aggaaatgcc tccagaagca
      421 aagatgagga tgaagaatat tggaagggat acaccaacat cagctggacc aaactccttc
      481 aataaaggaa agcatgggtt ttctgataac cagaagctgt gggagcgaaa tataaaatct
      541 catcttggaa atgtccatga ccaagacaat taaatgatgt tttgaaattg gggtgtgggg
      601 tgggtgtaaa gttaaaagga acagtttcct tttttaaaga atggtataag actatctttg
      661 gagccgcttt ttttttcttt ttcatttttt taaaagattg agtggtacac taataaatga
      721 gagtttgaaa ttagaggtaa tttatgtttt atatacagat ttcaagacat ttgctaattt
      781 tgtagtttca tgtgattagt ttccaaaggt tacagataat aaagaaatca gaaatggtac
      841 ctttttaaga attgcatatt tttttagaca caactattag tacattaaga gggaagcaaa
      901 gttactgtct atttaaaact gcaagcagtt aactctctta actcccttat tacctaaact
      961 tgtctggctc ccaggaacag ccttatagag agagggagta ttgtattggg aagaaaatgt
     1021 tactgaacta ttgactgaaa gtaaatttag ataaaataca gcttttttcc ttatgggcat
     1081 ttgttttgtt tcaagtcatc ataaactagg tattgcattg ctatccgtgg atacagacgc
     1141 ttagctctta aaagattttt tttttatgta aactgttgaa tatttgaaat agtccacttc
     1201 accttaatgg gtcttgtcta tcttcattag tcttcaaaga aaaaccattt gctaccaaag
     1261 taaatcagta ttttgaatgt gcttctcttg ttttttgttt attagctagt tcctgtaagc
     1321 atttccacca gaacttgagg caaatcgtaa ggaagctgtt tcttttaaaa cacaaaccac
     1381 caccaaaaat ttaaatgtac atattgctta agtatttggc tgtttttatt ttttaaaagg
     1441 tataaacacc aaaaaaaaaa ttaacattgt atgaagatgg aaaataagaa gatgcacttt
     1501 ctgtaacttt gtctaaggat ttaaattact aacttatgaa ctccaatttg aattgaactt
     1561 aactatcggc tttcttactg gtaaaattat atggtttatt ttaaatgcgt acatattgac
     1621 caatggcctc tgaaaaagca cattttagat actgaaattg aaggaaagaa aatgcatctt
     1681 caaacatttt ttggaatctc accacatata ctttgttaga tttgtgtatt gtagggtgtt
     1741 tgttttgtat ttttgtattg tatatgaact ttttttaaat gtgacagtta aacacatctt
     1801 taaaagcata gtcacagaca aaagcataca gtataaaaat ttccttgaaa actcctacaa
     1861 tattatattt ggaggcagct tcagactgtt ttattggtgg tagctgcttg ctgaggtctt
     1921 ttagttggta ataactccag agaagcagcc tgtgtatatt cctaacactt tgttcactag
     1981 catttaagtt tagaataagc ccaagtaaga caatggaaat gtatatagaa ctcttagttc
     2041 ttacatgatt taattatatc gatacatgaa tttaacttac tttaatgtag gcaaactatc
     2101 aattttttgt ccattttcct gtttgttaaa ataacatacc tctcctacgt attattttct
     2161 tgacccaaat gaaatattaa cctaaggtca agctgggaga gagaaatgac tgagatgaat
     2221 gtctttacta aagtaccaat aaatttgtca aactcaatag tgtttttatt ttctattcca
     2281 tttaa
//
