LOCUS       NM_001320399            2513 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens PEST proteolytic signal containing nuclear protein
            (PCNP), transcript variant 5, mRNA.
ACCESSION   NM_001320399
VERSION     NM_001320399.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Chen,Y.G., Liu,H.X., Hong,Y., Dong,P.Z., Liu,S.Y., Gao,Y.R., Lu,D.,
            Li,T., Wang,D.Y., Wu,D.D. and Ji,X.Y.
  TITLE     PCNP is a novel regulator of proliferation, migration, and invasion
            in human thyroid cancer
  JOURNAL   Int J Biol Sci 18 (9), 3605-3620 (2022)
   PUBMED   35813472
  REMARK    GeneRIF: PCNP is a novel regulator of proliferation, migration, and
            invasion in human thyroid cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Han,Z.P., Yang,Y., Chen,H.Y., Zhong,M.Y. and Zhuang,G.H.
  TITLE     TIPE2 and PCNP expression abnormalities in peripheral blood
            mononuclear cells associated with disease activity in rheumatoid
            arthritis: a meta-analysis
  JOURNAL   Eur Rev Med Pharmacol Sci 25 (3), 1242-1249 (2021)
   PUBMED   33629294
  REMARK    GeneRIF: TIPE2 and PCNP expression abnormalities in peripheral
            blood mononuclear cells associated with disease activity in
            rheumatoid arthritis: a meta-analysis.
            Review article
REFERENCE   3  (bases 1 to 2513)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2513)
  AUTHORS   Dong,P., Fu,H., Chen,L., Zhang,S., Zhang,X., Li,H., Wu,D. and Ji,X.
  TITLE     PCNP promotes ovarian cancer progression by accelerating
            beta-catenin nuclear accumulation and triggering EMT transition
  JOURNAL   J Cell Mol Med 24 (14), 8221-8235 (2020)
   PUBMED   32548978
  REMARK    GeneRIF: PCNP promotes ovarian cancer progression by accelerating
            beta-catenin nuclear accumulation and triggering EMT transition.
REFERENCE   5  (bases 1 to 2513)
  AUTHORS   Wu,D.D., Gao,Y.R., Li,T., Wang,D.Y., Lu,D., Liu,S.Y., Hong,Y.,
            Ning,H.B., Liu,J.P., Shang,J., Shi,J.F., Wei,J.S. and Ji,X.Y.
  TITLE     PEST-containing nuclear protein mediates the proliferation,
            migration, and invasion of human neuroblastoma cells through MAPK
            and PI3K/AKT/mTOR signaling pathways
  JOURNAL   BMC Cancer 18 (1), 499 (2018)
   PUBMED   29716528
  REMARK    GeneRIF: PCNP mediates the proliferation, migration, and invasion
            of human neuroblastoma cells through mitogen-activated protein
            kinase and PI3K/AKT/mTOR signaling pathways, implying that PCNP is
            a therapeutic target for patients with neuroblastoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2513)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   7  (bases 1 to 2513)
  AUTHORS   Mori,T., Li,Y., Hata,H. and Kochi,H.
  TITLE     NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP,
            a PEST-containing nuclear protein
  JOURNAL   FEBS Lett 557 (1-3), 209-214 (2004)
   PUBMED   14741369
  REMARK    GeneRIF: NIRF has ubiquitination activity, the hallmark of a
            ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7
            cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo.
REFERENCE   8  (bases 1 to 2513)
  AUTHORS   Mori,T., Li,Y., Hata,H., Ono,K. and Kochi,H.
  TITLE     NIRF, a novel RING finger protein, is involved in cell-cycle
            regulation
  JOURNAL   Biochem Biophys Res Commun 296 (3), 530-536 (2002)
   PUBMED   12176013
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA304502.1, BI596913.1, AC073861.29, AB037675.1 and BU608892.1.
            
            Transcript Variant: This variant (5) contains an alternate 5' exon
            and uses an alternate splice junction compared to variant 1. The
            resulting isoform (e) has a shorter and distinct N-terminus
            compared to isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.65689.1,
                                           SRR1803617.161414.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               DA304502.1         1-110
            111-430             BI596913.1         37-356
            431-461             AC073861.29        121074-121104
            462-619             BI596913.1         387-544
            620-2469            AB037675.1         390-2239
            2470-2513           BU608892.1         1-44                c
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q12.3"
     gene            1..2513
                     /gene="PCNP"
                     /note="PEST proteolytic signal containing nuclear protein"
                     /db_xref="GeneID:57092"
                     /db_xref="HGNC:HGNC:30023"
                     /db_xref="MIM:615210"
     exon            1..186
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            187..364
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..785
                     /gene="PCNP"
                     /note="isoform e is encoded by transcript variant 5;
                     PEST-containing nuclear protein"
                     /codon_start=1
                     /product="PEST proteolytic signal-containing nuclear
                     protein isoform e"
                     /protein_id="NP_001307328.1"
                     /db_xref="GeneID:57092"
                     /db_xref="HGNC:HGNC:30023"
                     /db_xref="MIM:615210"
                     /translation="MLFEVAAPINLLSKNSSSCNGGESSSRSAEKRSAEEEAADLPTK
                     PTKISKFGFAIGSQTTKKASAISIKLGSSKPKETVPTLAPKTLSVAAAFNEDEDSEPE
                     EMPPEAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQKLWERNIKSHLGNVHDQDN"
     exon            365..527
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            528..602
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            603..658
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     exon            659..2497
                     /gene="PCNP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2450..2455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCNP"
                     /note="hexamer: AATAAA"
     polyA_site      2469
                     /gene="PCNP"
                     /note="major polyA site"
     polyA_site      2497
                     /gene="PCNP"
ORIGIN      
        1 cttttcattc ctcgggaccg ctctaggctg ccaccacgcc ggctggcccc aaaaactcga
       61 tggttgttgg gcggggtcgt gacgtccttg gcgtggctgc aggggaggcc gcggcgggga
      121 aaatggcgga cgggaaggcg ggagacgaga agcctgaaaa gtcgcagcga gctggagccg
      181 ccggagttaa ttcatgggag cctcttctac tgccacacaa tctcagttcc tgctgtatac
      241 tctaacaaac agtggaagag actgttgggt cagtaggtga caactgcaga ggtatcttcc
      301 ttataacgat gctttttgag gttgctgctc ccatcaatct gctcagtaaa aatagctcat
      361 cttgtaatgg aggggaaagt tccagtcgca gcgctgagaa gcgatcagct gaagaagaag
      421 ctgccgacct cccaacaaag cctacaaaga tctccaagtt tggatttgcc ataggtagtc
      481 agacgacaaa gaaagcatca gccatatcca tcaaacttgg atcaagtaag cctaaagaaa
      541 ctgttccaac tcttgctcca aaaactcttt cagtagcagc agcttttaat gaagatgaag
      601 atagtgaacc agaggaaatg cctccagaag caaagatgag gatgaagaat attggaaggg
      661 atacaccaac atcagctgga ccaaactcct tcaataaagg aaagcatggg ttttctgata
      721 accagaagct gtgggagcga aatataaaat ctcatcttgg aaatgtccat gaccaagaca
      781 attaaatgat gttttgaaat tggggtgtgg ggtgggtgta aagttaaaag gaacagtttc
      841 cttttttaaa gaatggtata agactatctt tggagccgct ttttttttct ttttcatttt
      901 tttaaaagat tgagtggtac actaataaat gagagtttga aattagaggt aatttatgtt
      961 ttatatacag atttcaagac atttgctaat tttgtagttt catgtgatta gtttccaaag
     1021 gttacagata ataaagaaat cagaaatggt acctttttaa gaattgcata tttttttaga
     1081 cacaactatt agcacattaa gagggaagca aagttactgt ctatttaaaa ctgcaagcag
     1141 ttaactctct taactccctt attacctaaa cttgtctggc tcccaggaac agccttatag
     1201 agagagggag tattgtattg ggaagaaaat gttactgaac tattgactga aagtaaattt
     1261 agataaaata cagctttttt ccttatgggc atttgttttg tttcaagtca tcataaacta
     1321 ggtattgcat tgctatccgt ggatacagac gcttagctct taaaagattt tttttttatg
     1381 taaactgttg aatatttgaa atagtccact tcaccttaat gggtcttgtc tatcttcatt
     1441 agtcttcaaa gaaaaaccat ttgctaccaa agtaaatcag tattttgaat gtgcttctct
     1501 tgttttttgt ttattagcta gttcctgtaa gcatttccac cagaacttga ggcaaatcgt
     1561 aaggaagctg tttcttttaa aacacaaacc accaccaaaa atttaaatgt acatattgct
     1621 taagtatttg gctgttttta ttttttaaaa ggtataaaca ccaaaaaaaa aattaacatt
     1681 gtatgaagat ggaaaataag aagatgcact ttctgtaact ttgtctaagg atttaaatta
     1741 ctaacttatg aactccaatt tgaattgaac ttaactatcg gctttcttac tggtaaaatt
     1801 atatggttta ttttaaatgc gtacatattg accaatggcc tctgaaaaag cacattttag
     1861 atactgaaat tgaaggaaag aaaatgcatc ttcaaacatt ttttggaatc tcaccacata
     1921 tactttgtta gatttgtgta ttgtagggtg tttgttttgt atttttgtat tgtatatgaa
     1981 ctttttttaa atgtgacagt taaacacatc tttaaaagca tagtcacaga caaaagcata
     2041 cagtataaaa atttccttga aaactcctac aatattatat ttggaggcag cttcagactg
     2101 ttttattggt ggtagctgct tgctgaggtc ttttagttgg taataactcc agagaagcag
     2161 cctgtgtata ttcctaacac tttgttcact agcatttaag tttagaataa gcccaagtaa
     2221 gacaatggaa atgtatatag aactcttagt tcttacatga tttaattata tcgatacatg
     2281 aatttaactt actttaatgt aggcaaacta tcaatttttt gtccattttc ctgtttgtta
     2341 aaataacata cctctcctac gtattatttt cttgacccaa atgaaatatt aacctaaggt
     2401 caagctggga gagagaaatg actgagatga atgtctttac taaagtacca ataaatttgt
     2461 caaactcaat agtgttttta ttttctattc catttaaaaa aaaaaaaaaa aaa
//
