LOCUS       NM_182649               1276 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens proliferating cell nuclear antigen (PCNA), transcript
            variant 2, mRNA.
ACCESSION   NM_182649
VERSION     NM_182649.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Kim,S., Park,S.H., Kang,N., Ra,J.S., Myung,K. and Lee,K.Y.
  TITLE     Polyubiquitinated PCNA triggers SLX4-mediated break-induced
            replication in alternative lengthening of telomeres (ALT) cancer
            cells
  JOURNAL   Nucleic Acids Res 52 (19), 11785-11805 (2024)
   PUBMED   39291733
  REMARK    GeneRIF: Polyubiquitinated PCNA triggers SLX4-mediated
            break-induced replication in alternative lengthening of telomeres
            (ALT) cancer cells.
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Liu,D., Zuo,R., Liu,W., He,Y., Wang,Y., Yue,P., Gong,W., Cui,J.,
            Zhu,F., Luo,Y., Qi,L., Guo,Y., Chen,L., Li,G., Liu,Z., Chen,P. and
            Guo,H.
  TITLE     DNAJC24 acts directly with PCNA and promotes malignant progression
            of LUAD by activating phosphorylation of AKT
  JOURNAL   FASEB J 38 (9), e23630 (2024)
   PUBMED   38713100
  REMARK    GeneRIF: DNAJC24 acts directly with PCNA and promotes malignant
            progression of LUAD by activating phosphorylation of AKT.
REFERENCE   3  (bases 1 to 1276)
  AUTHORS   Yildirim,H., Turan,G. and Turan,M.
  TITLE     Expression of CD44, PCNA and E-cadherin in pterygium tissues
  JOURNAL   Indian J Ophthalmol 72 (Suppl 3), S501-S504 (2024)
   PUBMED   38648458
  REMARK    GeneRIF: Expression of CD44, PCNA and E-cadherin in pterygium
            tissues.
REFERENCE   4  (bases 1 to 1276)
  AUTHORS   He,Q., Wang,F., O'Donnell,M.E. and Li,H.
  TITLE     Cryo-EM reveals a nearly complete PCNA loading process and unique
            features of the human alternative clamp loader CTF18-RFC
  JOURNAL   Proc Natl Acad Sci U S A 121 (18), e2319727121 (2024)
   PUBMED   38669181
  REMARK    GeneRIF: Cryo-EM reveals a nearly complete PCNA loading process and
            unique features of the human alternative clamp loader CTF18-RFC.
REFERENCE   5  (bases 1 to 1276)
  AUTHORS   Huang,R., Nie,W., Mi,L., Yao,C. and Zhu,H.
  TITLE     EIF3B stabilizes PCNA by counteracting SYVN1-mediated
            ubiquitination to serve as a promotor in cholangiocarcinoma
  JOURNAL   Aging (Albany NY) 16 (8), 7311-7330 (2024)
   PUBMED   38687509
  REMARK    GeneRIF: EIF3B stabilizes PCNA by counteracting SYVN1-mediated
            ubiquitination to serve as a promotor in cholangiocarcinoma.
REFERENCE   6  (bases 1 to 1276)
  AUTHORS   Kemeny,M.M., Alava,G. and Oliver,J.M.
  TITLE     Improving responses in hepatomas with circadian-patterned hepatic
            artery infusions of recombinant interleukin-2
  JOURNAL   J Immunother (1991) 12 (4), 219-223 (1992)
   PUBMED   1477073
REFERENCE   7  (bases 1 to 1276)
  AUTHORS   Chen,M., Pan,Z.Q. and Hurwitz,J.
  TITLE     Sequence and expression in Escherichia coli of the 40-kDa subunit
            of activator 1 (replication factor C) of HeLa cells
  JOURNAL   Proc Natl Acad Sci U S A 89 (7), 2516-2520 (1992)
   PUBMED   1313560
REFERENCE   8  (bases 1 to 1276)
  AUTHORS   Webb,G., Parsons,P. and Chenevix-Trench,G.
  TITLE     Localization of the gene for human proliferating nuclear
            antigen/cyclin by in situ hybridization
  JOURNAL   Hum Genet 86 (1), 84-86 (1990)
   PUBMED   1979311
REFERENCE   9  (bases 1 to 1276)
  AUTHORS   Morris,G.F. and Mathews,M.B.
  TITLE     Analysis of the proliferating cell nuclear antigen promoter and its
            response to adenovirus early region 1
  JOURNAL   J Biol Chem 265 (27), 16116-16125 (1990)
   PUBMED   1975809
REFERENCE   10 (bases 1 to 1276)
  AUTHORS   Travali,S., Ku,D.H., Rizzo,M.G., Ottavio,L., Baserga,R. and
            Calabretta,B.
  TITLE     Structure of the human gene for the proliferating cell nuclear
            antigen
  JOURNAL   J Biol Chem 264 (13), 7466-7472 (1989)
   PUBMED   2565339
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK313286.1, BC000491.2 and
            BU626265.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 28, 2018 this sequence version replaced NM_182649.1.
            
            Summary: The protein encoded by this gene is found in the nucleus
            and is a cofactor of DNA polymerase delta. The encoded protein acts
            as a homotrimer and helps increase the processivity of leading
            strand synthesis during DNA replication. In response to DNA damage,
            this protein is ubiquitinated and is involved in the RAD6-dependent
            DNA repair pathway. Two transcript variants encoding the same
            protein have been found for this gene. Pseudogenes of this gene
            have been described on chromosome 4 and on the X chromosome.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.537031.1,
                                           SRR1803611.24070.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379143.10/ ENSP00000368438.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AK313286.1         1-15
            16-1271             BC000491.2         1-1256
            1272-1276           BU626265.1         18-22               c
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.3"
     gene            1..1276
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /note="proliferating cell nuclear antigen"
                     /db_xref="GeneID:5111"
                     /db_xref="HGNC:HGNC:8729"
                     /db_xref="MIM:176740"
     exon            1..381
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..946
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /note="cyclin; DNA polymerase delta auxiliary protein"
                     /codon_start=1
                     /product="proliferating cell nuclear antigen"
                     /protein_id="NP_872590.1"
                     /db_xref="CCDS:CCDS13087.1"
                     /db_xref="GeneID:5111"
                     /db_xref="HGNC:HGNC:8729"
                     /db_xref="MIM:176740"
                     /translation="MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSH
                     VSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALV
                     FEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVI
                     SCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKA
                     TPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS"
     exon            382..479
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            480..547
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            548..742
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            743..866
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1276
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1249..1254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /note="hexamer: AATAAA"
     polyA_site      1276
                     /gene="PCNA"
                     /gene_synonym="ATLD2"
                     /note="major polyA site"
ORIGIN      
        1 attaaacggt tgcaggcgta gcagagtggt cgttgtcttt ctaggtctca gccggtcgtc
       61 gcgacgttcg cccgctcgct ctgaggctcc tgaagccgaa accagctaga ctttcctcct
      121 tcccgcctgc ctgtagcggc gttgttgcca ctccgccacc atgttcgagg cgcgcctggt
      181 ccagggctcc atcctcaaga aggtgttgga ggcactcaag gacctcatca acgaggcctg
      241 ctgggatatt agctccagcg gtgtaaacct gcagagcatg gactcgtccc acgtctcttt
      301 ggtgcagctc accctgcggt ctgagggctt cgacacctac cgctgcgacc gcaacctggc
      361 catgggcgtg aacctcacca gtatgtccaa aatactaaaa tgcgccggca atgaagatat
      421 cattacacta agggccgaag ataacgcgga taccttggcg ctagtatttg aagcaccaaa
      481 ccaggagaaa gtttcagact atgaaatgaa gttgatggat ttagatgttg aacaacttgg
      541 aattccagaa caggagtaca gctgtgtagt aaagatgcct tctggtgaat ttgcacgtat
      601 atgccgagat ctcagccata ttggagatgc tgttgtaatt tcctgtgcaa aagacggagt
      661 gaaattttct gcaagtggag aacttggaaa tggaaacatt aaattgtcac agacaagtaa
      721 tgtcgataaa gaggaggaag ctgttaccat agagatgaat gaaccagttc aactaacttt
      781 tgcactgagg tacctgaact tctttacaaa agccactcca ctctcttcaa cggtgacact
      841 cagtatgtct gcagatgtac cccttgttgt agagtataaa attgcggata tgggacactt
      901 aaaatactac ttggctccca agatcgagga tgaagaagga tcttaggcat tcttaaaatt
      961 caagaaaata aaactaagct ctttgagaac tgcttctaag atgccagcat atactgaagt
     1021 cttttctgtc accaaatttg tacctctaag tacatatgta gatattgttt tctgtaaata
     1081 acctattttt ttctctattc tctgcaattt gtttaaagaa taaagtccaa agtcagatct
     1141 ggtctagtta acctagaagt atttttgtct cttagaaata cttgtgattt ttataataca
     1201 aaagggtctt gactctaaat gcagttttaa gaattgtttt tgaatttaaa taaagttact
     1261 tgaatttcaa acatca
//
