LOCUS       NM_018257               3859 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens protein-L-isoaspartate (D-aspartate)
            O-methyltransferase domain containing 2 (PCMTD2), transcript
            variant 1, mRNA.
ACCESSION   NM_018257
VERSION     NM_018257.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3859)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB224815.1, AK001745.1, BC033665.2 and CA443672.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018257.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.182872.1,
                                           SRR1660805.257041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308824.11/ ENSP00000307854.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DB224815.1         54-73
            21-182              AK001745.1         1-162
            183-3674            BC033665.2         61-3552
            3675-3859           CA443672.1         18-202              c
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..3859
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="protein-L-isoaspartate (D-aspartate)
                     O-methyltransferase domain containing 2"
                     /db_xref="GeneID:55251"
                     /db_xref="HGNC:HGNC:15882"
                     /db_xref="MIM:620077"
     exon            1..123
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     exon            124..454
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..1233
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein-L-isoaspartate O-methyltransferase
                     domain-containing protein 2"
                     /codon_start=1
                     /product="protein-L-isoaspartate O-methyltransferase
                     domain-containing protein 2 isoform 1"
                     /protein_id="NP_060727.2"
                     /db_xref="CCDS:CCDS13559.1"
                     /db_xref="GeneID:55251"
                     /db_xref="HGNC:HGNC:15882"
                     /db_xref="MIM:620077"
                     /translation="MGGAVSAGEDNDELIDNLKEAQYIRTELVEQAFRAIDRADYYLE
                     EFKENAYKDLAWKHGNIHLSAPCIYSEVMEALDLQPGLSFLNLGSGTGYLSSMVGLIL
                     GPFGVNHGVELHSDVIEYAKQKLDFFIRTSDSFDKFDFCEPSFVTGNCLEISPDCSQY
                     DRVYCGAGVQKEHEEYMKNLLKVGGILVMPLEEKLTKITRTGPSAWETKKILAVSFAP
                     LIQPCHSESGKSRLVQLPPVAVRSLQDLARIAIRGTIKKIIHQETVSKNGNGLKNTPR
                     FKRRRVRRRRMETIVFLDKEVFASRISNPSDDNSCEDLEEERREEEEKTPPETKPDPP
                     VNFLRQKVLSLPLPDPLKYYLLYYREK"
     exon            455..557
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     exon            558..729
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     exon            730..853
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     exon            854..3859
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3267..3272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="hexamer: AATAAA"
     polyA_site      3286
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="major polyA site"
     regulatory      3463..3468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="hexamer: AGTAAA"
     polyA_site      3490
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
     regulatory      3631..3636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="hexamer: AATAAA"
     polyA_site      3674
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
     regulatory      3837..3842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
                     /note="hexamer: AATAAA"
     polyA_site      3859
                     /gene="PCMTD2"
                     /gene_synonym="C20orf36"
ORIGIN      
        1 ggatgtttac ggcggccgag gttggagcgg cgctgctcgg ccgcggacac acgagggacg
       61 cgcccgagga gctgcaggtg gcagcccagg cggtccgaac ccgtcggccg gccgagcctg
      121 gagtattgcc taagtgtaat cttgaacatg ggcggtgctg tgagtgctgg tgaagacaat
      181 gatgagctga tagataattt gaaagaagca cagtatatcc ggactgagct ggtagagcag
      241 gctttcagag ctatcgatcg tgcagactat tatcttgaag aatttaaaga aaatgcttat
      301 aaagacttgg catggaagca tggaaacatt cacctctcag ccccgtgcat ctactcggag
      361 gtgatggaag ccctagatct gcagcctgga ctctcgtttc tgaacctggg cagtggcact
      421 gggtatctca gctccatggt gggcctcatt ctaggtcctt ttggtgtgaa ccatggggtg
      481 gaacttcact cagatgtgat agagtatgca aagcagaaac tggacttctt catcagaaca
      541 agtgatagtt ttgacaagtt tgacttctgt gaaccttcct ttgttactgg gaattgcctg
      601 gagatttctc cggattgttc tcagtatgat cgtgtatact gtggggctgg cgtgcagaaa
      661 gagcatgaag agtacatgaa gaatcttctc aaagtgggag ggatccttgt catgccactg
      721 gaagagaagt tgactaagat aacacgcaca ggtccttcag cttgggaaac caaaaagatt
      781 cttgctgttt cttttgctcc tctgatccag ccctgccatt cagagtcagg aaaatcaaga
      841 cttgtccagt taccaccagt ggcagttcgc agcctccagg acttggctcg catcgccatc
      901 cggggcacca ttaaaaagat tattcatcag gaaactgtga gcaaaaacgg aaacggacta
      961 aagaacaccc ccaggtttaa acgaaggaga gttcgccgcc gtcgaatgga aacgattgtc
     1021 tttttggaca aagaagtctt tgccagtcgg atttccaacc cctcagatga caacagctgt
     1081 gaagacttgg aagaggaacg gagggaagaa gaagagaaga ccccgccgga aacaaagcca
     1141 gaccccccag tgaacttcct acgccagaag gtcctgagcc tccctctgcc agatcccctg
     1201 aaatactact tgctttatta cagagaaaaa taagtctcct gtttgaaagg gggaaatagg
     1261 aagagcagat tgctgagtgt gaagttcgtg ctgcctgtgt gctgttgaag ggtcacctgg
     1321 aggcagacgt tgtggggaag ggaactgctg ggctcatcca caccatggtt ttcttctagt
     1381 tcctgattga cctctaaaat tctattcagt tgtatgattt gtttacatag ttccacaaga
     1441 ccttcattgc atagaagatt gttttcccaa agtggagaga atcttcatag agaaaaagag
     1501 aaggctgttt ctttttcggc tctgacgaaa cactgaagtc tgcgtaagag agactgtttg
     1561 atgaccgtcc ctcatgcaac atgcacggta ctcactaaaa atgaaaactg aagtggaaac
     1621 taacctgtgt tgcttataaa gtgtgaaagc acaagcttat aaatgtataa aatcttttct
     1681 gggtgtgacg cacctgcgtc caagtttgaa tttttatgat atgtaccact taattactgg
     1741 cactgagtat cactgaattt cttagttttc tagtggggaa acattattga gaagccctcc
     1801 cttattttaa gtaagttgat taaatcttat gtgagttgcc agttgtaatt tttcaaagga
     1861 aaaattttga tggggtggag gaatgaattg ccagataatc tttctggaat tccgagagaa
     1921 ttccaaagag ggtttttttt ttttttttta ggacatcttt tgataccttt aaaagaacca
     1981 ctgtcaagta atccttaaaa gaatatcttg gaaaaggaaa cagatttttt cctgtgtgta
     2041 agcaataagt gaagttacat ttgccctaac cctagggatg attctttacc cagttttaaa
     2101 gcccatcatg gtattctaag gtgttgacac cctccatcct cagagcaggt cgaaaatatt
     2161 aaatagactg gggactctat gatgggcagc ctgtgctttt tgacttcagt ttgctatttt
     2221 tctgtgatca cattagtact gattcataga ttctatcttt tataattctg gagaaaaaga
     2281 tttgttagtt ttgtaatttt tttgtaagac caaatgtatg tattttagta gctccattgc
     2341 atgagaagag tgtaactcac actgacttgt gatatcagcc ttctctgggc cttgtgtgtg
     2401 gagagctttc tatcttacca agtggtaggg ctaaaagaac aacagccttt ttggtagtca
     2461 catagcagaa tgatcagagt tacattgctt attccaaaac attggttctt tttaaaacat
     2521 ttttttttac ccaaagaaaa gaataataga aattactaac aataaatata aattcagagt
     2581 gttgatatag gattcagtat ccagagttta tttttaatct taatcctcag cttcttggga
     2641 gttgctgggc ttcagtgtct ctgtggtttc accagcttag cttgagctct ggttattttg
     2701 gatcttttct gcttttttta agtaactgag tcatttttac cacacagtcc agtttgcatg
     2761 tatagctagg aaacatgtat tgctctagat tgggcagttt aagtcatttt aaagaaagtt
     2821 agttcatagt tgttgccttt taactcatag tcaagcttca gtctttcaaa gagaaatgtg
     2881 tgattttcat ttacttgctg atattttgta gtttggagat ccttgtgggc attattctaa
     2941 ctgatacgta gacacttact tggaaatttt tggacattat attaaatgag tgctatctgt
     3001 gaaattggtt atattaggtg gcttgactaa tgttttttct ataattgtat atggactgca
     3061 tttttaaaaa aaccgcattt gcctttatgc tagattgtaa aaaattatat tagaatgcat
     3121 aagacatgtt tttccttcat atgctagact tttcctagca tttcgtattt ctgtgttgtc
     3181 agtgtgtgat ttttaaaccg gaatttggtt taaaaaaaat ctggtggtaa tatatgtgag
     3241 aaatactttg gtgtttacct tatgaaaata aaggattgta agtaaagttt cctgcgcacc
     3301 ttataccaga attcagtata atacactact ttctgttttc aaacagataa atcataatat
     3361 agtctgtatt atctgtaaga tctgtcttgt aaaccacatt cttgacaact atttgctttt
     3421 gagtagtttg tattttaata tgtgactttt gtcttgaaaa gtagtaaagc catagacttg
     3481 tgcaaaacaa gtttcaagtt tatagatatt aagtttgtaa tgtgagcatc aaatgtgtat
     3541 gtaaaaatac tttttaccag tctggaactt gggaaaatcc agggaatttg aaacatagat
     3601 tttaatgagc tggtaaacac aaatcatgtc aataaaggta gtcaggatat tttatcctta
     3661 gcattgcttc tgcatcctgt gtaggattcc aattcttgaa tatgttcttt tcaaaatctt
     3721 aagaaaagaa ccttttttct ttattaacat catgtgttta ctttcagcaa atatttgtat
     3781 tactgcttga ttctgtgaca gtcacaatag atgtagagaa ggcattattt ttcattaata
     3841 aatgtgaaat tggaatgga
//
