LOCUS       NM_006197               8652 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pericentriolar material 1 (PCM1), transcript variant
            1, mRNA.
ACCESSION   NM_006197
VERSION     NM_006197.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 8652)
  AUTHORS   Wang,L., Paudyal,S.C., Kang,Y., Owa,M., Liang,F.X., Spektor,A.,
            Knaut,H., Sanchez,I. and Dynlacht,B.D.
  TITLE     Regulators of tubulin polyglutamylation control nuclear shape and
            cilium disassembly by balancing microtubule and actin assembly
  JOURNAL   Cell Res 32 (2), 190-209 (2022)
   PUBMED   34782749
REFERENCE   4  (bases 1 to 8652)
  AUTHORS   Liu,W.N., Wu,K.X., Wang,X.T., Lin,L.R., Tong,M.L. and Liu,L.L.
  TITLE     LncRNA-ENST00000421645 promotes T cells to secrete IFN-gamma by
            sponging PCM1 in neurosyphilis
  JOURNAL   Epigenomics 13 (15), 1187-1203 (2021)
   PUBMED   34382410
  REMARK    GeneRIF: LncRNA-ENST00000421645 promotes T cells to secrete
            IFN-gamma by sponging PCM1 in neurosyphilis.
REFERENCE   5  (bases 1 to 8652)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 8652)
  AUTHORS   Corvi,R., Berger,N., Balczon,R. and Romeo,G.
  TITLE     RET/PCM-1: a novel fusion gene in papillary thyroid carcinoma
  JOURNAL   Oncogene 19 (37), 4236-4242 (2000)
   PUBMED   10980597
REFERENCE   7  (bases 1 to 8652)
  AUTHORS   Engelender,S., Sharp,A.H., Colomer,V., Tokito,M.K., Lanahan,A.,
            Worley,P., Holzbaur,E.L. and Ross,C.A.
  TITLE     Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued
            subunit of dynactin
  JOURNAL   Hum Mol Genet 6 (13), 2205-2212 (1997)
   PUBMED   9361024
REFERENCE   8  (bases 1 to 8652)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
REFERENCE   9  (bases 1 to 8652)
  AUTHORS   Ohata,H., Fujiwara,Y., Koyama,K. and Nakamura,Y.
  TITLE     Mapping of the human autoantigen pericentriolar material 1 (PCM1)
            gene to chromosome 8p21.3-p22
  JOURNAL   Genomics 24 (2), 404-406 (1994)
   PUBMED   7698772
REFERENCE   10 (bases 1 to 8652)
  AUTHORS   Balczon,R., Bao,L. and Zimmer,W.E.
  TITLE     PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle
            distribution
  JOURNAL   J Cell Biol 124 (5), 783-793 (1994)
   PUBMED   8120099
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087273.5 and AC087625.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_006197.3.
            
            Summary: The protein encoded by this gene is a component of
            centriolar satellites, which are electron dense granules scattered
            around centrosomes. Inhibition studies show that this protein is
            essential for the correct localization of several centrosomal
            proteins, and for anchoring microtubules to the centrosome.
            Chromosomal aberrations involving this gene are associated with
            papillary thyroid carcinomas and a variety of hematological
            malignancies, including atypical chronic myeloid leukemia and
            T-cell lymphoma. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Oct
            2015].
            
            Transcript Variant: Variants 1, 24, and 25 encode isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.199778.1,
                                           SRR1803611.211583.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325083.13/ ENSP00000327077.8
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AC087273.5         21663-21863         c
            202-269             AC087273.5         20071-20138         c
            270-387             AC087273.5         9145-9262           c
            388-633             AC087273.5         7472-7717           c
            634-903             AC087273.5         5842-6111           c
            904-1074            AC087273.5         4990-5160           c
            1075-1252           AC087625.12        64792-64969
            1253-1362           AC087625.12        68221-68330
            1363-1579           AC087625.12        70576-70792
            1580-1763           AC087625.12        73076-73259
            1764-1937           AC087625.12        74210-74383
            1938-2095           AC087625.12        74870-75027
            2096-2331           AC087625.12        75146-75381
            2332-2483           AC087625.12        77620-77771
            2484-2613           AC087625.12        77921-78050
            2614-2754           AC087625.12        79640-79780
            2755-2945           AC087625.12        80707-80897
            2946-3146           AC087625.12        82174-82374
            3147-3366           AC087625.12        83605-83824
            3367-3512           AC087625.12        83934-84079
            3513-3703           AC087625.12        84586-84776
            3704-3875           AC087625.12        87183-87354
            3876-4234           AC087625.12        89935-90293
            4235-4399           AC087625.12        98197-98361
            4400-4572           AC087625.12        103053-103225
            4573-4701           AC087625.12        103565-103693
            4702-4822           AC087625.12        107465-107585
            4823-4981           AC087625.12        109148-109306
            4982-5118           AC087625.12        111089-111225
            5119-5253           AC087625.12        123869-124003
            5254-5451           AC087625.12        127153-127350
            5452-5511           AC087625.12        128215-128274
            5512-5641           AC087625.12        128843-128972
            5642-5802           AC087625.12        129273-129433
            5803-5875           AC087625.12        131570-131642
            5876-6132           AC087625.12        132190-132446
            6133-6225           AC087625.12        142967-143059
            6226-6340           AC087625.12        143150-143264
            6341-8652           AC087625.12        145243-147554
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..8652
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="pericentriolar material 1"
                     /db_xref="GeneID:5108"
                     /db_xref="HGNC:HGNC:8727"
                     /db_xref="MIM:600299"
     exon            1..201
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            202..269
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            270..387
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..6366
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pericentriolar material 1 protein; pericentriolar material
                     1, PCM1; PCM-1; hPCM-1"
                     /codon_start=1
                     /product="pericentriolar material 1 protein isoform 1"
                     /protein_id="NP_006188.4"
                     /db_xref="CCDS:CCDS47812.1"
                     /db_xref="GeneID:5108"
                     /db_xref="HGNC:HGNC:8727"
                     /db_xref="MIM:600299"
                     /translation="MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRS
                     SEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEK
                     LKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNP
                     PNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITK
                     ASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENL
                     KKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSI
                     TSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDE
                     KVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGL
                     APVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVH
                     YYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNA
                     QCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEE
                     EEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDD
                     DAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQ
                     QQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTV
                     NQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGL
                     AETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVR
                     TDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTR
                     QQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCL
                     LQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQ
                     HPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFP
                     SNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEV
                     ESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFS
                     SMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESAS
                     MSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMF
                     EALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISE
                     SHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNAS
                     VLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDG
                     AGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKE
                     HMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDC
                     GEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILE
                     DHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSIN
                     LSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRD
                     FKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQ
                     PNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKS
                     DEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEP
                     ETVGAQSI"
     exon            388..633
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            634..903
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1074
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1252
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1362
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1579
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1763
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1937
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2095
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2331
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2483
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2484..2613
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2754
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2945
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3146
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3366
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..3512
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3703
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3704..3875
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3876..4234
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4235..4399
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4400..4572
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4701
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4702..4822
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4823..4981
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4982..5118
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5119..5253
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5254..5451
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5452..5511
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5512..5641
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5642..5802
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5803..5875
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5876..6132
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            6133..6225
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            6226..6340
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            6341..8652
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6432..6437
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="hexamer: AATAAA"
     polyA_site      6462
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="major polyA site"
     regulatory      6652..6657
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="hexamer: AATAAA"
     polyA_site      6679
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
     regulatory      8629..8634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="hexamer: AATAAA"
     regulatory      8633..8638
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
                     /note="hexamer: AATATA"
     polyA_site      8647
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
     polyA_site      8652
                     /gene="PCM1"
                     /gene_synonym="PTC4; RET/PCM-1"
ORIGIN      
        1 acaatgagat cctaagatgg cggtggctgc ggcggttggc gctgcgtagc tgaggtcgaa
       61 aaggcggcca ctggggccga ggcagccagg aaacgtgtgg gcctctctgc tgcggtctcc
      121 gagggccgac cgctgccggc ggcgggtcgt gggggctgac tgtcgctctg cctttgacag
      181 gagaggctgc ttcttgtaga ggaaacagct ttgaagtgtg gagcgggaaa ggagcagttt
      241 ctgagctgca aaaactagtt tctaaacaga gagttaattg ttaaatccag tatggccaca
      301 ggaggaggtc cctttgaaga tggcatgaat gatcaggatt taccaaactg gagtaatgag
      361 aatgttgatg acaggctcaa caatatggat tggggtgccc aacagaagaa agcaaataga
      421 tcatcagaaa agaataagaa aaagtttggt gtagaaagtg ataaaagagt aaccaatgat
      481 atttctccgg agtcgtcacc aggagttgga aggcgaagaa caaagactcc acatacgttc
      541 ccacacagta gatacatgag tcagatgtct gtcccagagc aggcagaatt agagaaactg
      601 aaacagcgga taaacttcag tgatttagat cagagaagca ttggaagtga ttcccaaggt
      661 agagcaacag ctgctaacaa caaacgtcag cttagtgaaa accgaaagcc cttcaacttt
      721 ttgcctatgc agattaatac taacaagagc aaagatgcat ctacaaaccc cccaaacaga
      781 gaaacgattg gatcagcaca gtgtaaagag ttgtttgctt ctgctttaag taatgacctc
      841 ttgcaaaact gtcaggtgtc tgaagaagat gggaggggag aacctgcaat ggagagcagc
      901 cagattgtaa gcaggcttgt tcaaattcgc gattatatta ctaaagctag ttccatgcgg
      961 gaagatcttg tagagaaaaa tgagagatct gctaatgttg agcgccttac tcatctaata
     1021 gatcacctta aagaacaaga gaagtcatat atgaaatttc ttaaaaaaat ccttgccaga
     1081 gatcctcagc aggagcctat ggaagagata gaaaatttga agaaacaaca tgatttatta
     1141 aaaagaatgt tacaacagca ggagcaacta agagctctac agggacggca ggctgcactt
     1201 ctagctctgc aacataaagc agagcaagct attgcagtga tggatgattc tgttgttgca
     1261 gaaactgcag gtagcttatc tggcgtcagt atcacatctg aactaaatga agaattgaat
     1321 gacttaattc agcgttttca taatcagctt cgtgattctc agcctccagc tgttccagac
     1381 aatagaagac aggcagaaag tctttcatta actagggagg tttcccagag caggaaacca
     1441 tcagcttcag aacgtttacc tgatgagaaa gtcgaacttt ttagcaaaat gagagtgcta
     1501 caggaaaaga aacaaaaaat ggacaaattg cttggagaac ttcatacact tcgagatcag
     1561 catcttaaca attcatcatc ctctccacaa aggagtgtcg atcagagaag tacttcagct
     1621 ccctctgctt ctgtaggctt ggcaccggtt gtcaatggag aatccaatag cctcacatca
     1681 tctgttcctt atcctactgc ttctctagta tctcagaatg agagtgaaaa cgaaggccac
     1741 ctcaatccat ctgaaaaact ccagaagtta aatgaagttc gaaagagatt gaatgagcta
     1801 agagaattag ttcattatta tgaacaaacg tcagacatga tgacagatgc tgtgaatgaa
     1861 aacaggaaag atgaagaaac tgaagagtca gaatatgatt ctgagcatga aaattccgag
     1921 cctgttacta acattcgaaa tccacaagta gcttccactt ggaatgaagt aaatagtcat
     1981 agtaatgcac agtgtgtttc taataataga gatgggcgaa cagttaattc taattgtgaa
     2041 attaacaaca gatctgctgc caacataagg gctctaaaca tgcctccttc tttagattgt
     2101 cgatataata gagaagggga acaggagatt catgttgcac aaggtgaaga tgatgaggag
     2161 gaggaggaag aagcagaaga ggagggagtc agtggagctt cattatctag tcacaggagc
     2221 agtctggttg atgagcatcc agaagatgct gaatttgaac agaagatcaa ccgacttatg
     2281 gctgcaaaac agaaacttag acagttacaa gatcttgttg ctatggtaca ggatgatgat
     2341 gcagctcaag gagttatctc tgccagtgca tcaaatttgg atgatttcta cccagcagaa
     2401 gaagacacca agcaaaattc aaataacact agaggaaatg ccaataaaac acagaaagat
     2461 actggagtaa atgaaaaggc aagagagaaa ttttatgagg ctaaactaca gcagcaacag
     2521 agagagctaa aacaattgca ggaagaaaga aagaaactga ttgacattca ggagaaaatt
     2581 caagcattgc aaacggcatg ccctgactta cagctgtcag ctgctagtgt gggtaactgt
     2641 cccaccaaaa aatatatgcc agctgttact tcaaccccaa ctgttaatca acacgagacc
     2701 agtacaagca aatctgtttt tgagcctgaa gattcttcaa tagtagataa tgagttgtgg
     2761 tcagaaatga gaagacatga aatgttgagg gaggagctgc gacagagaag aaagcagctt
     2821 gaagctctga tggctgaaca tcagaggagg caaggtctag ctgaaactgc atctccagtg
     2881 gctgtgtcat tgagaagtga tggatctgag aacctatgta ctcctcagca aagtagaaca
     2941 gaaaaaacga tggcaacttg gggagggtct acccagtgtg cactagatga agaaggagat
     3001 gaagacggtt acctttctga aggaattgtt cggacagatg aagaggagga agaagagcaa
     3061 gatgccagtt ccaatgataa cttttctgtg tgtccttcta acagtgtgaa tcataactcc
     3121 tacaatggaa aggaaactaa aaataggtgg aagaacaatt gccctttttc ggcagatgaa
     3181 aattatcgtc ctttagccaa gacaaggcaa cagaatatca gcatgcaacg gcaagaaaac
     3241 cttcgttggg tgtcagagct ctcttacgta gaagagaaag aacaatggca agaacaaatc
     3301 aatcagctaa agaaacagct tgattttagt gtcagtattt gtcagacttt gatgcaagac
     3361 cagcagactc tatcttgtct gctacaaact cttctcacgg gtccttacag tgttatgccc
     3421 agcaatgttg catctcctca agtacacttc ataatgcacc agttgaacca gtgctatact
     3481 cagctaacat ggcaacagaa taatgttcag aggttgaaac aaatgctaaa tgaacttatg
     3541 cgccagcaaa atcagcatcc agaaaaacct ggaggcaagg aaagaggcag tagtgcatcg
     3601 caccctcctt ctcccagttt attttgtcct ttcagctttc caacacagcc tgtaaatctc
     3661 ttcaatatac ctggatttac taacttttca tcatttgcac caggtatgaa tttcagccct
     3721 ttatttcctt ctaattttgg agatttttct cagaatatct ctacacccag tgaacagcag
     3781 caacccttag cccagaattc ttcaggaaaa acagaatata tggcttttcc aaaacctttt
     3841 gaaagcagtt cctctattgg agcagagaaa ccaaggaata aaaaactgcc tgaagaggag
     3901 gtggaaagca gtaggacacc atggttatat gaacaagaag gtgaagtaga gaaaccattt
     3961 atcaagactg gattttcagt gtctgtagaa aaatctacaa gtagtaaccg caaaaatcaa
     4021 ttagatacaa acggaagaag acgccagttt gatgaagaat cactggaaag ctttagcagt
     4081 atgcctgatc cagtagatcc aacaacagtg actaaaacat tcaagacaag aaaagcgtct
     4141 gcacaggcca gcctggcatc taaagataaa actcccaagt caaaaagtaa gaagaggaat
     4201 tctactcagc tgaaaagcag agttaaaaac atcaggtatg aaagtgccag tatgtctagc
     4261 acatgtgaac cttgcaaaag taggaacaga cattcagccc agactgaaga gcctgttcaa
     4321 gcaaaagtat tcagcagaaa gaatcatgag caactggaaa aaataataaa atgtaatagg
     4381 tctacagaaa tatcttcaga aactgggagt gatttttcca tgtttgaagc tttgcgagat
     4441 actatttatt ctgaagtagc tacattaatt tctcaaaatg aatctcgtcc acattttctt
     4501 attgaactct tccatgagct gcagctacta aacacagact acttgagaca gagggcttta
     4561 tatgcattgc aggacatagt atccagacat atttctgaga gccatgaaaa aggagaaaat
     4621 gtaaagtcag taaactctgg tacttggata gcatcaaact cagaacttac tcctagtgag
     4681 agccttgcta ctactgatga tgaaactttt gagaagaact ttgaaagaga aacccataaa
     4741 ataagtgagc aaaatgatgc tgataatgct agtgtcctgt ctgtatcatc aaattttgag
     4801 ccttttgcaa cagatgatct aggtaacacc gtgattcact tagatcaagc attagccaga
     4861 atgagagaat atgagcgtat gaagactgag gctgaaagta actcaaatat gagatgcacc
     4921 tgcaggatta ttgaggatgg agatggtgct ggtgcaggta ctacagttaa taatttagaa
     4981 gaaactcccg ttattgaaaa tcgtagttca caacaacctg taagtgaagt ttctaccatc
     5041 ccatgtccta gaattgatac tcagcagctg gaccggcaaa ttaaagcaat tatgaaagaa
     5101 gtcattcctt ttttgaagga gcacatggat gaagtatgct cctcgcagct tctaacttca
     5161 gtaaggcgca tggttttgac ccttacccag caaaatgatg agagcaaaga gtttgtaaag
     5221 ttctttcata aacaacttgg aagtatatta caggattcac tggcaaaatt tgctggcaga
     5281 aaactgaaag actgtggaga agatcttctt gtagagatat ctgaagtgtt gttcaatgaa
     5341 ttggctttct ttaagcttat gcaagatttg gataataata gtataactgt taaacagaga
     5401 tgcaaaagga aaatagaagc aactggagtg atacaatctt gtgccaaaga ggctaaaagg
     5461 attcttgaag atcatggctc acctgctgga gagattgatg atgaagacaa agacaaggat
     5521 gaaactgaaa cagttaagca gactcaaaca tctgaggtgt atgatggtcc caaaaatgta
     5581 agatctgata tttctgatca agaggaagat gaagaaagtg aaggatgtcc agtgtctatt
     5641 aatttgtcta aagctgaaac tcaggcttta actaattatg gaagtggaga agatgaaaat
     5701 gaggatgaag aaatggaaga atttgaagaa ggccctgtgg atgtccagac ttccctccag
     5761 gctaacactg aagctactga agaaaatgaa catgatgaac aggtcctaca acgtgacttt
     5821 aaaaagacag cagaaagcaa aaatgtccca ttggaacgag aagccactag taaaaatgac
     5881 caaaataact gtcctgtgaa accctgttac ctcaatatct tggaagatga gcaaccttta
     5941 aatagtgctg cccataagga gtcacctcct actgttgatt caactcaaca gcctaaccct
     6001 ttgccgttac gtttacctga aatggaaccc ttagtgccta gagtcaaaga agttaaatct
     6061 gctcaggaaa ctcctgaaag ctctctggct ggaagtcctg atactgaatc tccagtgtta
     6121 gtgaatgact atgaagcaga atctggtaat ataagtcaaa agtctgatga agaagatttt
     6181 gtaaaagttg aagatttacc actgaaactg acaatatatt cagaggcaga tctaagaaag
     6241 aaaatggtag aagaagaaca gaaaaaccat ttatctggtg aaatatgtga aatgcagacc
     6301 gaagaattag ctggaaattc tgagacacta aaagaacctg aaacggtggg agcccagagt
     6361 atatgagatg tcttcagagg ctcatctaac tctgtcctta catactcaat gcatatatga
     6421 aaacaatact aaataaacat ctgatctgta taaaaatgta aattagtttg acactgcttt
     6481 tttgataggt gtggtcattt ctccccatgg tagtttaaaa catcagaaac tgaattctgg
     6541 acagatttaa gccttgacac actgtgtttt tttttttttc ccccttcttt tttgggtctt
     6601 catttttttc cccattgtga tgtttggtaa cgaatttaaa atgtagtttt aaataaagtt
     6661 tggacttatc tataaagtat cttttttgga aattatattg aattctatac agcaagtcaa
     6721 tgttttatat aactttaggc tgctcagaga agagcaatgg ttaagagtta gttagagaaa
     6781 tatattattt gttataaagc ccatccatta ggccagtctt ccaactaatg ccagtgttgc
     6841 tgctgttggg tctgatgttc ttcttttaga tacctgcagg tcctattcct gtgcaagaat
     6901 agggcagatt atcaagatat ccaggatacc tatgaagtta ttatagaata tttattaatc
     6961 cattgaaatt ggataataag tttagaacat aggttctcag tatctagaac ttacatcatt
     7021 atcatctgtt tgttaggatt tgaaattctg gaaaatattt atctacatcg cctcagacta
     7081 aaagtaaaaa aaataagctt tatataatta gggagatttc tgcacagaga agtaacattg
     7141 tggttaattt taaatgaaaa acttaacttt ttcaagtggg gataaataat acaactaaat
     7201 ttctgtaata gtaagattct gtatgccttc agataaactt gcctattgag atggtaattt
     7261 aaagccaaag catagcagtt tcttttgtgt gtagtgaggt tgaagcagat ttgcaggtga
     7321 agtattgaaa gtttatgtga ctttaagtca gcttttgaaa agtgattgat ttgcttttta
     7381 tcccaaactg tccatatacc cagtaaggct tcaaaaaacc agtcaacaat gagtaagtca
     7441 atcttataga ttcttcttcc tcgaataaaa tacaaagaat tagttccaat aagtatttta
     7501 actttgttaa caactgaaat accccataaa aaaagaactt gttgagagta tttctttaaa
     7561 atggttactt gctgcccagg caccatggct cactcctgta atcccagcac cttgggaggc
     7621 cgaggcgggc agatcacgag gtcaagagat tgagaccatc ctgcccaaca tgggggaaac
     7681 cccgtctcta ctaaaaataa aaaaattagc tgggagaggt ggcacgtgcc tgtagtccta
     7741 gctacttggg aggctaaggc aggagaatag cctgaacccg ggaggtggag gttgcaggtt
     7801 gcagtgagcc gagatcatgc cactgcactc cagcctggca acagagcgag actctgtctc
     7861 aaaaaaaaaa aaaaaaaaaa aaaaaagtta acttgctgta tacctcagtg taatgtccat
     7921 tcaaggagta ttaaatgagg atttccctgc gaggacattt actgtattgc tacttaaatt
     7981 atggaagaca atatatcttc aactttaata aaacctattc agaaaattac caattcagaa
     8041 ttcggagttc ttatccaggt gctctaacta acttcaggga aattggaaca ataagttatg
     8101 ttacatgcac actcaaattc tttattttct ccactttaag caggaaaggg taaaaactgt
     8161 tttggtactc aagcccagcc ttacatactg tgtttctctc tctgtctgca tgcatattaa
     8221 agtggaaaaa ttgtatttat atcttagtta ttaccatagt acctatgaac cttatcaaaa
     8281 ttgcttattt gactggtgtt acagctgcta ttaatctaag tctattgttt ttctatttta
     8341 gtagataatt tagttttaaa atacgtaggg tttgagagca gatatattta tttaatctgt
     8401 tttctctagt aactattgct gaagggttag gcattcagta ttcctatttg tcctaatttt
     8461 gaagttaaaa attttggtta cagatagata gagggagaaa agttcaaaat gagtgagaga
     8521 gaactttatg caggttgaga taatgcctaa aataatgagc tggccagact gtggaggtac
     8581 tctttgtatt ttgtaacatt gactttgggt aaatgctttt tcactgttaa taaatatata
     8641 tcctgtatac aa
//
