LOCUS       NM_020418               1962 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens poly(rC) binding protein 4 (PCBP4), transcript variant
            1, mRNA.
ACCESSION   NM_020418
VERSION     NM_020418.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   3  (bases 1 to 1962)
  AUTHORS   Ito,Y., Narita,N., Nomi,N., Sugimoto,C., Takabayashi,T., Yamada,T.,
            Karaya,K., Matsumoto,H. and Fujieda,S.
  TITLE     Suppression of Poly(rC)-Binding Protein 4 (PCBP4) reduced cisplatin
            resistance in human maxillary cancer cells
  JOURNAL   Sci Rep 5, 12360 (2015)
   PUBMED   26196957
  REMARK    GeneRIF: PCBP4 plays important roles in the induction of cisplatin
            resistance in human maxillary cancers.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1962)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (bases 1 to 1962)
  AUTHORS   Scoumanne,A., Cho,S.J., Zhang,J. and Chen,X.
  TITLE     The cyclin-dependent kinase inhibitor p21 is regulated by
            RNA-binding protein PCBP4 via mRNA stability
  JOURNAL   Nucleic Acids Res 39 (1), 213-224 (2011)
   PUBMED   20817677
REFERENCE   6  (bases 1 to 1962)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     The poly(C)-binding proteins: a multiplicity of functions and a
            search for mechanisms
  JOURNAL   RNA 8 (3), 265-278 (2002)
   PUBMED   12003487
  REMARK    Review article
REFERENCE   7  (bases 1 to 1962)
  AUTHORS   Tchernev,V.T., Mansfield,T.A., Giot,L., Kumar,A.M., Nandabalan,K.,
            Li,Y., Mishra,V.S., Detter,J.C., Rothberg,J.M., Wallace,M.R.,
            Southwick,F.S. and Kingsmore,S.F.
  TITLE     The Chediak-Higashi protein interacts with SNARE complex and signal
            transduction proteins
  JOURNAL   Mol Med 8 (1), 56-64 (2002)
   PUBMED   11984006
REFERENCE   8  (bases 1 to 1962)
  AUTHORS   Taylor,W.R. and Stark,G.R.
  TITLE     Regulation of the G2/M transition by p53
  JOURNAL   Oncogene 20 (15), 1803-1815 (2001)
   PUBMED   11313928
  REMARK    Review article
REFERENCE   9  (bases 1 to 1962)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     Identification of two novel mammalian genes establishes a subfamily
            of KH-domain RNA-binding proteins
  JOURNAL   Genomics 67 (3), 301-316 (2000)
   PUBMED   10936052
REFERENCE   10 (bases 1 to 1962)
  AUTHORS   Zhu,J. and Chen,X.
  TITLE     MCG10, a novel p53 target gene that encodes a KH domain RNA-binding
            protein, is capable of inducing apoptosis and cell cycle arrest in
            G(2)-M
  JOURNAL   Mol Cell Biol 20 (15), 5602-5618 (2000)
   PUBMED   10891498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC115284.4, DA701799.1 and
            BC017098.1.
            
            On Jun 2, 2019 this sequence version replaced NM_020418.3.
            
            Summary: This gene encodes a member of the KH-domain protein
            subfamily. Proteins of this subfamily, also referred to as
            alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine
            regions. Alpha-CPs play important roles in post-transcriptional
            activities and have different cellular distributions. This gene is
            induced by the p53 tumor suppressor, and the encoded protein can
            suppress cell proliferation by inducing apoptosis and cell cycle
            arrest in G(2)-M. This gene's protein is found in the cytoplasm,
            yet it lacks the nuclear localization signals found in other
            subfamily members. Multiple alternatively spliced transcript
            variants have been described, but the full-length nature for only
            some has been determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the shorter isoform
            (a). The 5' UTR may be incomplete due to lack of 5'-complete
            transcript support for this variant, and the presence of
            alternative splicing choices further upstream.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017098.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC115284.4         49322-49341         c
            21-25               DA701799.1         68-72
            26-1962             BC017098.1         1-1937
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.2"
     gene            1..1962
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="poly(rC) binding protein 4"
                     /db_xref="GeneID:57060"
                     /db_xref="HGNC:HGNC:8652"
                     /db_xref="MIM:608503"
     exon            1..129
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            130..212
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            213..357
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..1359
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="isoform a is encoded by transcript variant 1; RNA
                     binding protein MCG10; LYST-interacting protein;
                     alpha-CP4"
                     /codon_start=1
                     /product="poly(rC)-binding protein 4 isoform a"
                     /protein_id="NP_065151.2"
                     /db_xref="CCDS:CCDS2840.2"
                     /db_xref="GeneID:57060"
                     /db_xref="HGNC:HGNC:8652"
                     /db_xref="MIM:608503"
                     /translation="MSGSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKKGETVKRIR
                     EQSSARITISEGSCPERITTITGSTAAVFHAVSMIAFKLDEDLCAAPANGGNVSRPPV
                     TLRLVIPASQCGSLIGKAGTKIKEIRESPPKGATIPYHPSLSLGTVLLSANQGFSVQG
                     QYGAVTPAEVTKLQQLSSHAVPFATPSVVPGLDPGTQTSSQEFLVPNDLIGCVIGRQG
                     SKISEIRQMSGAHIKIGNQAEGAGERHVTITGSPVSIALAQYLITACLETAKSTSGGT
                     PSSAPADLPAPFSPPLTALPTAPPGLLGTPYAISLSNFIGLKPMPFLALPPASPGPPP
                     GLAAYTAKMAAANGSKKAERQKFSPY"
     exon            358..381
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            382..414
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            415..531
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            532..663
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            664..738
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            739..783
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            784..847
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            848..900
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1070
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1962
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1942..1947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="hexamer: AATAAA"
     polyA_site      1962
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="major polyA site"
ORIGIN      
        1 aaacccctca ttgttcgcag ctgatgtcac tcgcagttgt gagcggccgc ctctcccggg
       61 gacaatgtgg gactgagcgg cccagccgcc gtgccgccgc cgccgccgcc gcaggacagc
      121 cccagcgagc ctctggagtc cctgtggcag aactcaaatg gaaggaggag cagctgcacc
      181 caggggagca gggtgtcacc tccgttggat agagccggcc aggttgcagc gcggacacac
      241 tcgcaggtcg ctgtggcccc agcctcgcct gacagaatga gcggctcgga cgggggactg
      301 gaggaggagc cagagctcag catcaccctc acgctgcgga tgctgatgca cgggaaggaa
      361 gtgggcagca tcatcgggaa gaagggcgag actgtaaagc gaatccggga gcagagcagt
      421 gcccggatca ccatctccga gggctcctgc cctgaacgca tcaccaccat caccgggtct
      481 acagcagctg tcttccatgc agtctccatg attgctttca aactggatga ggacctttgt
      541 gctgctcctg caaatggtgg aaatgtctcc aggcctccag tgaccctgcg ccttgtcatc
      601 cctgccagtc agtgtggctc actgattggg aaggctggca ccaagatcaa ggagatccga
      661 gagtccccac ccaaaggagc cactatcccc taccatccga gcctctccct aggtactgtt
      721 cttctctctg ccaaccaggg cttctctgtc cagggtcagt atggggctgt gaccccagct
      781 gaggtcacca agctccagca gctctcaagc catgcggtcc cctttgccac acccagcgtg
      841 gtgccaggac tggatcccgg cacacagacc agctcacagg agttcttggt tcccaacgat
      901 ttgattggct gtgtgatcgg gcgccagggc agcaagatca gcgagatccg gcagatgtca
      961 ggggcacata tcaagatcgg gaaccaagca gagggcgctg gggagcggca tgtcaccatc
     1021 actggctctc cggtctccat cgccctggcc cagtacctca tcactgcctg tctagagacg
     1081 gccaagtcta cctctggggg gacgcccagc tcggcccccg cagacctgcc tgcccccttc
     1141 tcgccacccc tgacggccct gcccacagct ccccctggcc tgctgggcac accctatgcc
     1201 atctccctct ccaacttcat cggcctcaag cccatgccct tcttggcttt accacctgct
     1261 tccccagggc cgccgccggg cttggcggcc tacactgcca agatggcagc agctaatggg
     1321 agcaagaagg ctgagcggca gaaattctcc ccctactgag gccagctgag gtacaggcag
     1381 gggcaggcag gaccaccagc agggggctgc ctctgcaccc tacccgccca aggagactcc
     1441 accctggggt cccaaacgcc gctaacgccc agacgcatgg atgcaccccc taccctgcct
     1501 ccatctatgg gagttctttc tctcagagtg ggggcagttt ctggcccagg ggtctgagct
     1561 gcggcagccc cagggcaggg ggccctacct cctcagctct gtgcttggat acagggagca
     1621 gccaggagac tccctagtgc ccccaccatg gcgggtgtca ctcacgcact ccccatccct
     1681 tagggcttcc tggcctactg catccttgtg ggagtcaggg aggagggccc gttgggtagc
     1741 tggggccagg cttctctccc caccacctgc agatttcttg ctgcttccac tgataccctt
     1801 ttgactggaa tgaactggct gggcttgtca gggggcaccc caaagagggg gcactgccag
     1861 gtagctgggg gagtggcatg gggcaggggc ccagttctca gcagcagaca ctctgtacag
     1921 ttttttcaat ccctgttttt gaataaatat tctcagcgac ca
//
