LOCUS       NM_001174100            2156 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens poly(rC) binding protein 4 (PCBP4), transcript variant
            5, mRNA.
ACCESSION   NM_001174100
VERSION     NM_001174100.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   3  (bases 1 to 2156)
  AUTHORS   Ito,Y., Narita,N., Nomi,N., Sugimoto,C., Takabayashi,T., Yamada,T.,
            Karaya,K., Matsumoto,H. and Fujieda,S.
  TITLE     Suppression of Poly(rC)-Binding Protein 4 (PCBP4) reduced cisplatin
            resistance in human maxillary cancer cells
  JOURNAL   Sci Rep 5, 12360 (2015)
   PUBMED   26196957
  REMARK    GeneRIF: PCBP4 plays important roles in the induction of cisplatin
            resistance in human maxillary cancers.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2156)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (bases 1 to 2156)
  AUTHORS   Scoumanne,A., Cho,S.J., Zhang,J. and Chen,X.
  TITLE     The cyclin-dependent kinase inhibitor p21 is regulated by
            RNA-binding protein PCBP4 via mRNA stability
  JOURNAL   Nucleic Acids Res 39 (1), 213-224 (2011)
   PUBMED   20817677
REFERENCE   6  (bases 1 to 2156)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     The poly(C)-binding proteins: a multiplicity of functions and a
            search for mechanisms
  JOURNAL   RNA 8 (3), 265-278 (2002)
   PUBMED   12003487
  REMARK    Review article
REFERENCE   7  (bases 1 to 2156)
  AUTHORS   Tchernev,V.T., Mansfield,T.A., Giot,L., Kumar,A.M., Nandabalan,K.,
            Li,Y., Mishra,V.S., Detter,J.C., Rothberg,J.M., Wallace,M.R.,
            Southwick,F.S. and Kingsmore,S.F.
  TITLE     The Chediak-Higashi protein interacts with SNARE complex and signal
            transduction proteins
  JOURNAL   Mol Med 8 (1), 56-64 (2002)
   PUBMED   11984006
REFERENCE   8  (bases 1 to 2156)
  AUTHORS   Taylor,W.R. and Stark,G.R.
  TITLE     Regulation of the G2/M transition by p53
  JOURNAL   Oncogene 20 (15), 1803-1815 (2001)
   PUBMED   11313928
  REMARK    Review article
REFERENCE   9  (bases 1 to 2156)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     Identification of two novel mammalian genes establishes a subfamily
            of KH-domain RNA-binding proteins
  JOURNAL   Genomics 67 (3), 301-316 (2000)
   PUBMED   10936052
REFERENCE   10 (bases 1 to 2156)
  AUTHORS   Zhu,J. and Chen,X.
  TITLE     MCG10, a novel p53 target gene that encodes a KH domain RNA-binding
            protein, is capable of inducing apoptosis and cell cycle arrest in
            G(2)-M
  JOURNAL   Mol Cell Biol 20 (15), 5602-5618 (2000)
   PUBMED   10891498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA074216.1, AK225815.1 and
            AC115284.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001174100.1.
            
            Summary: This gene encodes a member of the KH-domain protein
            subfamily. Proteins of this subfamily, also referred to as
            alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine
            regions. Alpha-CPs play important roles in post-transcriptional
            activities and have different cellular distributions. This gene is
            induced by the p53 tumor suppressor, and the encoded protein can
            suppress cell proliferation by inducing apoptosis and cell cycle
            arrest in G(2)-M. This gene's protein is found in the cytoplasm,
            yet it lacks the nuclear localization signals found in other
            subfamily members. Multiple alternatively spliced transcript
            variants have been described, but the full-length nature for only
            some has been determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (5) differs in the 5' UTR, and
            includes an additional in-frame exon in the central coding region,
            compared to variant 1. The encoded isoform (c) is longer than
            isoform a. Variants 3, 4 and 5 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225815.1, SRR14038193.3674551.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000461554.6/ ENSP00000417196.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA074216.1         13-21
            10-183              AK225815.1         1-174
            184-184             AC115284.4         43921-43921         c
            185-516             AK225815.1         176-507
            517-517             AC115284.4         42492-42492         c
            518-2156            AK225815.1         508-2146
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.2"
     gene            1..2156
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="poly(rC) binding protein 4"
                     /db_xref="GeneID:57060"
                     /db_xref="HGNC:HGNC:8652"
                     /db_xref="MIM:608503"
     exon            1..129
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            130..277
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            278..422
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     CDS             342..1553
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="isoform c is encoded by transcript variant 5; RNA
                     binding protein MCG10; LYST-interacting protein;
                     alpha-CP4"
                     /codon_start=1
                     /product="poly(rC)-binding protein 4 isoform c"
                     /protein_id="NP_001167571.1"
                     /db_xref="CCDS:CCDS2839.1"
                     /db_xref="GeneID:57060"
                     /db_xref="HGNC:HGNC:8652"
                     /db_xref="MIM:608503"
                     /translation="MSGSDGGLEEEPELSITLTLRMLMHGKEVGSIIGKKGETVKRIR
                     EQSSARITISEGSCPERITTITGSTAAVFHAVSMIAFKLDEDLCAAPANGGNVSRPPV
                     TLRLVIPASQCGSLIGKAGTKIKEIRETTGAQVQVAGDLLPNSTERAVTVSGVPDAII
                     LCVRQICAVILESPPKGATIPYHPSLSLGTVLLSANQGFSVQGQYGAVTPAEVTKLQQ
                     LSSHAVPFATPSVVPGLDPGTQTSSQEFLVPNDLIGCVIGRQGSKISEIRQMSGAHIK
                     IGNQAEGAGERHVTITGSPVSIALAQYLITACLETAKSTSGGTPSSAPADLPAPFSPP
                     LTALPTAPPGLLGTPYAISLSNFIGLKPMPFLALPPASPGPPPGLAAYTAKMAAANGS
                     KKAERQKFSPY"
     exon            423..446
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            447..479
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            480..596
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            597..728
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            729..857
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            858..932
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            933..977
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1041
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1094
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1264
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..2156
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2136..2141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="hexamer: AATAAA"
     polyA_site      2156
                     /gene="PCBP4"
                     /gene_synonym="CBP; LIP4; MCG10"
                     /note="major polyA site"
ORIGIN      
        1 aaacccctca ttgttcgcag ctgatgtcac tcgcagttgt gagcggccgc ctctcccggg
       61 gacaatgtgg gactgagcgg cccagccgcc gtgccgccgc cgccgccgcc gcaggacagc
      121 cccagcgagg ccatttccag cacatagaag agagattgga aaccaacgtg cagaactgcc
      181 agtcccctga cacgctgtgc cccacccact gcagcccagt gctgaatgaa ccctgcccag
      241 aggtgtctgt agtgagcttc tgccctagtg acttttgagc cggccaggtt gcagcgcgga
      301 cacactcgca ggtcgctgtg gccccagcct cgcctgacag aatgagcggc tcggacgggg
      361 gactggagga ggagccagag ctcagcatca ccctcacgct gcggatgctg atgcacggga
      421 aggaagtggg cagcatcatc gggaagaagg gcgagactgt aaagcgaatc cgggagcaga
      481 gcagtgcccg gatcaccatc tccgagggct cctgccctga acgcatcacc accatcaccg
      541 ggtctacagc agctgtcttc catgcagtct ccatgattgc tttcaaactg gatgaggacc
      601 tttgtgctgc tcctgcaaat ggtggaaatg tctccaggcc tccagtgacc ctgcgccttg
      661 tcatccctgc cagtcagtgt ggctcactga ttgggaaggc tggcaccaag atcaaggaga
      721 tccgagagac tacgggtgcc caggtacagg tggcagggga cctgctcccc aactccacag
      781 agcgagctgt tacggtatct ggggtgcctg atgccatcat cctgtgtgtg cgccagatct
      841 gcgctgttat cctggagtcc ccacccaaag gagccactat cccctaccat ccgagcctct
      901 ccctaggtac tgttcttctc tctgccaacc agggcttctc tgtccagggt cagtatgggg
      961 ctgtgacccc agctgaggtc accaagctcc agcagctctc aagccatgcg gtcccctttg
     1021 ccacacccag cgtggtgcca ggactggatc ccggcacaca gaccagctca caggagttct
     1081 tggttcccaa cgatttgatt ggctgtgtga tcgggcgcca gggcagcaag atcagcgaga
     1141 tccggcagat gtcaggggca catatcaaga tcgggaacca agcagagggc gctggggagc
     1201 ggcatgtcac catcactggc tctccggtct ccatcgccct ggcccagtac ctcatcactg
     1261 cctgtctaga gacggccaag tctacctctg gggggacgcc cagctcggcc cccgcagacc
     1321 tgcctgcccc cttctcgcca cccctgacgg ccctgcccac agctccccct ggcctgctgg
     1381 gcacacccta tgccatctcc ctctccaact tcatcggcct caagcccatg cccttcttgg
     1441 ctttaccacc tgcttcccca gggccgccgc cgggcttggc ggcctacact gccaagatgg
     1501 cagcagctaa tgggagcaag aaggctgagc ggcagaaatt ctccccctac tgaggccagc
     1561 tgaggtacag gcaggggcag gcaggaccac cagcaggggg ctgcctctgc accctacccg
     1621 cccaaggaga ctccaccctg gggtcccaaa cgccgctaac gcccagacgc atggatgcac
     1681 cccctaccct gcctccatct atgggagttc tttctctcag agtgggggca gtttctggcc
     1741 caggggtctg agctgcggca gccccagggc agggggccct acctcctcag ctctgtgctt
     1801 ggatacaggg agcagccagg agactcccta gtgcccccac catggcgggt gtcactcacg
     1861 cactccccat cccttagggc ttcctggcct actgcatcct tgtgggagtc agggaggagg
     1921 gcccgttggg tagctggggc caggcttctc tccccaccac ctgcagattt cttgctgctt
     1981 ccactgatac ccttttgact ggaatgaact ggctgggctt gtcagggggc accccaaaga
     2041 gggggcactg ccaggtagct gggggagtgg catggggcag gggcccagtt ctcagcagca
     2101 gacactctgt acagtttttt caatccctgt ttttgaataa atattctcag cgacca
//
