LOCUS       NM_001384156            2282 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens poly(rC) binding protein 3 (PCBP3), transcript variant
            25, mRNA.
ACCESSION   NM_001384156
VERSION     NM_001384156.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Qiu,X., Shi,Q., Zhang,X., Shi,X., Jiang,H. and Qin,S.
  TITLE     LncRNA A2M-AS1 Promotes Ferroptosis in Pancreatic Cancer via
            Interacting With PCBP3
  JOURNAL   Mol Cancer Res 20 (11), 1636-1645 (2022)
   PUBMED   35920801
  REMARK    GeneRIF: LncRNA A2M-AS1 Promotes Ferroptosis in Pancreatic Cancer
            via Interacting With PCBP3.
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2282)
  AUTHORS   Ger,M., Kaupinis,A., Petrulionis,M., Kurlinkus,B., Cicenas,J.,
            Sileikis,A., Valius,M. and Strupas,K.
  TITLE     Proteomic Identification of FLT3 and PCBP3 as Potential Prognostic
            Biomarkers for Pancreatic Cancer
  JOURNAL   Anticancer Res 38 (10), 5759-5765 (2018)
   PUBMED   30275197
  REMARK    GeneRIF: Low PCBP3 expression is associated with Pancreatic ductal
            adenocarcinoma.
REFERENCE   4  (bases 1 to 2282)
  AUTHORS   Wang,Y., Gao,L., Tse,S.W. and Andreadis,A.
  TITLE     Heterogeneous nuclear ribonucleoprotein E3 modestly activates
            splicing of tau exon 10 via its proximal downstream intron, a
            hotspot for frontotemporal dementia mutations
  JOURNAL   Gene 451 (1-2), 23-31 (2010)
   PUBMED   19914360
  REMARK    GeneRIF: Study examined splicing factors located on chromosome 21
            for their effect on tau exon 10 and discovered that one of them,
            hnRNPE3 (PCBP3), modestly activates splicing of exon 10 by
            interacting with its proximal downstream intron around position
            +19.
REFERENCE   5  (bases 1 to 2282)
  AUTHORS   Chkheidze,A.N. and Liebhaber,S.A.
  TITLE     A novel set of nuclear localization signals determine distributions
            of the alphaCP RNA-binding proteins
  JOURNAL   Mol Cell Biol 23 (23), 8405-8415 (2003)
   PUBMED   14612387
REFERENCE   6  (bases 1 to 2282)
  AUTHORS   Makeyev,A.V., Eastmond,D.L. and Liebhaber,S.A.
  TITLE     Targeting a KH-domain protein with RNA decoys
  JOURNAL   RNA 8 (9), 1160-1173 (2002)
   PUBMED   12358435
REFERENCE   7  (bases 1 to 2282)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     The poly(C)-binding proteins: a multiplicity of functions and a
            search for mechanisms
  JOURNAL   RNA 8 (3), 265-278 (2002)
   PUBMED   12003487
  REMARK    Review article
REFERENCE   8  (bases 1 to 2282)
  AUTHORS   Makeyev,A.V. and Liebhaber,S.A.
  TITLE     Identification of two novel mammalian genes establishes a subfamily
            of KH-domain RNA-binding proteins
  JOURNAL   Genomics 67 (3), 301-316 (2000)
   PUBMED   10936052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133493.3, AL133492.2,
            AL592528.1, AJ011931.1 and AJ239328.2.
            
            Summary: This gene encodes a member of the KH-domain protein
            subfamily. Proteins of this subfamily, also referred to as
            alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine
            regions. Alpha-CPs play important roles in post-transcriptional
            activities and have different cellular distributions. The protein
            encoded by this gene lacks the nuclear localization signals found
            in other subfamily members. Alternative splicing results in
            multiple transcript variants encoding distinct isoforms. [provided
            by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (25) differs in the 5' UTR
            compared to variant 1. Variants 1, 14, and 17-25 all encode the
            same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372079.83489.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000681687.1/ ENSP00000505796.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AL133493.3         141487-141630
            145-223             AL133492.2         4051-4129
            224-261             AL133492.2         70569-70606
            262-297             AL133492.2         90594-90629
            298-394             AL592528.1         37332-37428
            395-432             AJ011931.1         74006-74043         c
            433-587             AJ011931.1         27739-27893         c
            588-611             AJ011931.1         24479-24502         c
            612-644             AJ011931.1         23478-23510         c
            645-761             AJ011931.1         22988-23104         c
            762-893             AJ011931.1         14615-14746         c
            894-1022            AJ011931.1         13071-13199         c
            1023-1097           AJ239328.2         607-681
            1098-1139           AJ239328.2         4244-4285
            1140-1218           AJ239328.2         16573-16651
            1219-1278           AJ239328.2         17442-17501
            1279-1331           AJ239328.2         21909-21961
            1332-1501           AJ239328.2         26686-26855
            1502-2282           AJ239328.2         28326-29106
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..2282
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /note="poly(rC) binding protein 3"
                     /db_xref="GeneID:54039"
                     /db_xref="HGNC:HGNC:8651"
                     /db_xref="MIM:608502"
     exon            1..144
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            145..223
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            224..261
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            262..297
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            298..432
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     CDS             423..1538
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /note="isoform 1 is encoded by transcript variant 25;
                     poly(rC) binding protein 3 overlapping transcript; PCBP3
                     overlapping transcript 1; PCBP3-overlapping transcript"
                     /codon_start=1
                     /product="poly(rC)-binding protein 3 isoform 1"
                     /protein_id="NP_001371085.1"
                     /db_xref="CCDS:CCDS42974.2"
                     /db_xref="GeneID:54039"
                     /db_xref="HGNC:HGNC:8651"
                     /db_xref="MIM:608502"
                     /translation="MGEGDAFWAPSVLPHSTLSTLSHHPQPQFGRRMESKVSEGGLNV
                     TLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFK
                     AFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTG
                     AQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPYRPKPASTP
                     VIFAGGQAYTIQGQYAIPHPDQLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEE
                     AQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATE
                     GSSERQITITGTPANISLAQYLINARLTSEVTGMGTL"
     exon            433..587
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            588..611
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            612..644
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            645..761
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            762..893
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1022
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1097
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1139
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1218
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1278
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1331
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1501
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..2282
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2263..2268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /note="hexamer: AATAAA"
     polyA_site      2282
                     /gene="PCBP3"
                     /gene_synonym="ALPHA-CP3; PCBP3-OT1; PCBP3OT"
                     /note="major polyA site"
ORIGIN      
        1 gcctcagccg cctcagccgc ggtcgccgcc gcttcggctg acttaggctc cgccgccgcc
       61 tcctcacccg cctcgccccg accccgcccg ggcctcccgg cctctccccg ccggcccgcg
      121 ggctcgccgt ccggcgcagg gcagcacatt ggagatatat ttccacagca tctggctttt
      181 cttgttcctg ttctgaagta aatgtaactg ctatcctctg gaggactgtc tacaagagag
      241 aagctcccca cgtggttccg tgtaaatacc taggagtggg cttttctaga tcacatggtc
      301 ggtaggctcc acgacaaaag tcaacccttc tgtaaatcac ctgctgtggt tatgatgctc
      361 tgagttcaat acgtctgaac ctttgctgtc tatggatctg ctctaaacct tatagcctgc
      421 ttatggggga aggtgacgcc ttctgggccc catctgtcct tcctcacagc accctcagca
      481 ccttaagcca ccaccctcag ccacaatttg gcagaaggat ggagtccaag gtctcagaag
      541 gtggcctgaa tgtgaccctc accatccgcc tgctgatgca tggaaaggaa gttggaagca
      601 tcatcgggaa gaaaggagaa actgtgaaga agatgcgtga ggagagtggt gcaaggatca
      661 acatctcaga gggaaactgc ccagagagga ttgtgaccat cacaggcccc acagacgcca
      721 tcttcaaggc ctttgccatg atcgcataca agtttgagga ggatatcatc aactccatga
      781 gcaacagccc tgccaccagc aagcccccag tgacgctgag gctggtggtg cctgccagcc
      841 agtgtgggtc cctgatcggc aaaggaggct ccaagatcaa ggagatcagg gagtccacag
      901 gtgcccaggt gcaggtggct ggggacatgc tgcccaactc cacggagcga gcggtgacca
      961 tctcggggac cccagatgcc atcatccagt gcgtcaagca gatctgtgtg gtcatgctgg
     1021 agtccccacc gaaaggtgcc accattccct accgcccaaa gcccgcctcc acccctgtca
     1081 tttttgcagg tggtcaggcc tacacaatcc agggacagta tgccatccct cacccggatc
     1141 agttgaccaa gctccaccag ttggccatgc agcaaacccc ctttcctccc ctcggacaga
     1201 ccaaccccgc tttccccgga gaaaagctgc ctttacactc ctccgaagaa gctcaaaatc
     1261 tgatgggcca gtcatcaggt ctggacgcca gcccaccggc cagcactcat gagctcacca
     1321 ttcccaatga tctaataggc tgcataattg gacgccaagg gaccaaaatc aatgaaattc
     1381 gacagatgtc tggagctcag atcaaaatcg ccaacgccac ggaagggtcc tcagagcgtc
     1441 agatcaccat cacggggacc ccggccaaca tcagccttgc ccagtatctc atcaacgcca
     1501 ggctgacgtc cgaggtcacc gggatgggca cgctgtaatc ctacccagca cccttccccc
     1561 gcgtcaccca cctgccagag cctaaggccc ccggctctcg cactctgtac agcccacctt
     1621 ccctgcctca cagataccaa tagagaggtt ttcttaatta acaaaaggac gtatgccatg
     1681 gagaaacaca cccgcgcaca cagctgctct ctacagaggc tgcaggctcc gccgagtccc
     1741 ccctcagtgt tattttattt atgacttacg ctcccgtctg cccatgcacc ggcatgcagt
     1801 ggtaattatt ttagaaatat tgttccttgg tgtcagcgta gctgtctgtc ttaggagctg
     1861 ggtcggcgtt ccgacagcac ttcctgtccg cccttctcct ctgccatcca gaaccgtcca
     1921 gaactgttgc ctgagacccc tcctctctca cacagccctg ccatgctgac tcggtttccc
     1981 ctcagagcca ttgttgtctg ggctcgagtt tctgccccag gttgtgtgct ggaatcgggg
     2041 ggtggctctc ctgccaccca tggggagcgc caggagagga gggtcatgga ggatgttggg
     2101 gctctgaccc caggagtggg gtggagggcg gagcctgctg ggggccctgc cttcacagag
     2161 atgccgcgtg ctgggaaggc tcttggggtc ccctgagcgt cttccagggt ggctggagag
     2221 cacagacgcg ccagggagcc ccctctgtgc tcctcagagt tcaataaatg tcgtggcccc
     2281 tc
//
