LOCUS       NM_031989               3159 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens poly(rC) binding protein 2 (PCBP2), transcript variant
            2, mRNA.
ACCESSION   NM_031989
VERSION     NM_031989.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Jia,W., Yu,L., Xu,B., Feng,Y., Wang,J., Zhu,D., Xu,C., Liang,L.,
            Zhou,Y., Kong,L. and Ding,W.
  TITLE     HNF4A-AS1 inhibits the progression of hepatocellular carcinoma by
            promoting the ubiquitin-modulated degradation of PCBP2 and
            suppressing the stability of ARG2 mRNA
  JOURNAL   Int J Biol Sci 20 (13), 5087-5108 (2024)
   PUBMED   39430249
  REMARK    GeneRIF: HNF4A-AS1 inhibits the progression of hepatocellular
            carcinoma by promoting the ubiquitin-modulated degradation of PCBP2
            and suppressing the stability of ARG2 mRNA.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Qu,T., Cha,L., Liu,H., Tian,L., Hu,X., Zou,H., Feng,Y., Sun,C.,
            Cao,J., Guo,W., Qiu,F. and Zhou,B.
  TITLE     Circ_0005397 inhibits ferroptosis of pancreatic cancer cells by
            up-regulating PCBP2 through KAT6A/H3K9Ac
  JOURNAL   FASEB J 38 (17), e70028 (2024)
   PUBMED   39235355
  REMARK    GeneRIF: Circ_0005397 inhibits ferroptosis of pancreatic cancer
            cells by up-regulating PCBP2 through KAT6A/H3K9Ac.
REFERENCE   3  (bases 1 to 3159)
  AUTHORS   Li,Y. and Liu,L.
  TITLE     UKLF/PCBP2 axis governs the colorectal cancer development by
            transcriptionally activating SLC39A4
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (6), 119755 (2024)
   PUBMED   38768927
  REMARK    GeneRIF: UKLF/PCBP2 axis governs the colorectal cancer development
            by transcriptionally activating SLC39A4.
REFERENCE   4  (bases 1 to 3159)
  AUTHORS   Mi,D., Yanatori,I., Zheng,H., Kong,Y., Hirayama,T. and Toyokuni,S.
  TITLE     Association of poly(rC)-binding protein-2 with sideroflexin-3
            through TOM20 as an iron entry pathway to mitochondria
  JOURNAL   Free Radic Res 58 (4), 261-275 (2024)
   PUBMED   38599240
  REMARK    GeneRIF: Association of poly(rC)-binding protein-2 with
            sideroflexin-3 through TOM20 as an iron entry pathway to
            mitochondria.
REFERENCE   5  (bases 1 to 3159)
  AUTHORS   Scott,S., Li,Y., Bermek,O., Griffith,J.D., Lemon,S.M. and Choi,K.H.
  TITLE     Binding of microRNA-122 to the hepatitis C virus 5' untranslated
            region modifies interactions with poly(C) binding protein 2 and the
            NS5B viral polymerase
  JOURNAL   Nucleic Acids Res 51 (22), 12397-12413 (2023)
   PUBMED   37941151
  REMARK    GeneRIF: Binding of microRNA-122 to the hepatitis C virus 5'
            untranslated region modifies interactions with poly(C) binding
            protein 2 and the NS5B viral polymerase.
REFERENCE   6  (bases 1 to 3159)
  AUTHORS   Blyn,L.B., Towner,J.S., Semler,B.L. and Ehrenfeld,E.
  TITLE     Requirement of poly(rC) binding protein 2 for translation of
            poliovirus RNA
  JOURNAL   J Virol 71 (8), 6243-6246 (1997)
   PUBMED   9223526
REFERENCE   7  (bases 1 to 3159)
  AUTHORS   Blyn,L.B., Swiderek,K.M., Richards,O., Stahl,D.C., Semler,B.L. and
            Ehrenfeld,E.
  TITLE     Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus
            RNA 5' noncoding region: identification by automated liquid
            chromatography-tandem mass spectrometry
  JOURNAL   Proc Natl Acad Sci U S A 93 (20), 11115-11120 (1996)
   PUBMED   8855318
REFERENCE   8  (bases 1 to 3159)
  AUTHORS   Tommerup,N. and Leffers,H.
  TITLE     Assignment of human KH-box-containing genes by in situ
            hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13,
            and PCBP2 to 12q13.12-q13.13, distal to FRA12A
  JOURNAL   Genomics 32 (2), 297-298 (1996)
   PUBMED   8833161
REFERENCE   9  (bases 1 to 3159)
  AUTHORS   Kiledjian,M., Wang,X. and Liebhaber,S.A.
  TITLE     Identification of two KH domain proteins in the alpha-globin mRNP
            stability complex
  JOURNAL   EMBO J 14 (17), 4357-4364 (1995)
   PUBMED   7556077
REFERENCE   10 (bases 1 to 3159)
  AUTHORS   Leffers,H., Dejgaard,K. and Celis,J.E.
  TITLE     Characterisation of two major cellular poly(rC)-binding human
            proteins, each containing three K-homologous (KH) domains
  JOURNAL   Eur J Biochem 230 (2), 447-453 (1995)
   PUBMED   7607214
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB188306.1, AK292141.1,
            AK130583.1, AC023509.42 and BF509278.1.
            
            On Jun 2, 2019 this sequence version replaced NM_031989.4.
            
            Summary: The protein encoded by this gene appears to be
            multifunctional. Along with PCBP-1 and hnRNPK, it is one of the
            major cellular poly(rC)-binding proteins. The encoded protein
            contains three K-homologous (KH) domains which may be involved in
            RNA binding. Together with PCBP-1, this protein also functions as a
            translational coactivator of poliovirus RNA via a sequence-specific
            interaction with stem-loop IV of the IRES, promoting poliovirus RNA
            replication by binding to its 5'-terminal cloverleaf structure. It
            has also been implicated in translational control of the
            15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and
            hepatitis A virus RNA. The encoded protein is also suggested to
            play a part in formation of a sequence-specific alpha-globin mRNP
            complex which is associated with alpha-globin mRNA stability. This
            multiexon structural mRNA is thought to be retrotransposed to
            generate PCBP-1, an intronless gene with functions similar to that
            of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and
            PCBP4) which are thought to have arisen as a result of duplication
            events of entire genes. This gene also has two processed
            pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jan 2018].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site compared to variant 1. The resulting isoform (b) has
            the same N- and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.36783.1,
                                           SRR3476690.641470.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000546463.6/ ENSP00000448762.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-223               AB188306.1         1-223
            224-1680            AK292141.1         1-1457
            1681-2804           AK130583.1         813-1936
            2805-2855           AC023509.42        141912-141962       c
            2856-3159           BF509278.1         17-320              c
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..3159
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="poly(rC) binding protein 2"
                     /db_xref="GeneID:5094"
                     /db_xref="HGNC:HGNC:8648"
                     /db_xref="MIM:601210"
     exon            1..275
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            276..419
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             351..1439
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="isoform b is encoded by transcript variant 2;
                     alpha-CP2; heterogenous nuclear ribonucleoprotein E2;
                     hnRNP E2; heterogeneous nuclear ribonucleoprotein E2;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="poly(rC)-binding protein 2 isoform b"
                     /protein_id="NP_114366.1"
                     /db_xref="CCDS:CCDS8859.1"
                     /db_xref="GeneID:5094"
                     /db_xref="HGNC:HGNC:8648"
                     /db_xref="MIM:601210"
                     /translation="MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESG
                     ARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRL
                     VVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVK
                     QICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDL
                     EGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWA
                     GLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTI
                     TGSAASISLAQYLINVRLSSETGGMGSS"
     exon            420..443
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            444..476
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..593
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            594..725
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..854
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            855..929
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1022
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1064
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1137
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1176
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1232
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1402
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..3159
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1775..1780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="hexamer: ATTAAA"
     polyA_site      1845
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="major polyA site"
     regulatory      2783..2788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="hexamer: AGTAAA"
     polyA_site      2805
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="major polyA site"
     regulatory      2877..2882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="hexamer: TATAAA"
     polyA_site      2899
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
     regulatory      3140..3145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
                     /note="hexamer: TATAAA"
     polyA_site      3159
                     /gene="PCBP2"
                     /gene_synonym="hnRNP-E2; HNRNPE2; HNRPE2"
ORIGIN      
        1 cccagaccag cagaggcagc agccggagca gccgcagcct gcgccctctc ccgcccgccc
       61 gccctccgcc cgcccgcccg ccctccgccg ccctccaccc gccccggggt ctctttcccc
      121 cttcctcctc ctcctcctcc accccccctt cctcctccgc ccgcccgcgg ggcccccctc
      181 gccttcccgc ccgcccctat tgttccgccc ccggcctccc gcccttcccc ttcccgcccg
      241 ctcccctttt cccctcagtc gcctcgcgcc tgcagttttt ggctttcacc cccaaccagt
      301 gaccaaagac ttgaccactc aaagtccagc tccccagaac actgctcgac atggacaccg
      361 gtgtgattga aggtggatta aatgtcactc tcaccatccg gctacttatg catggaaagg
      421 aagttggcag tatcatcgga aagaaaggag aatcagttaa gaagatgcgc gaggagagtg
      481 gtgcacgtat caacatctca gaagggaatt gtcctgagag aattatcact ttggctggac
      541 ccactaatgc catcttcaaa gcctttgcta tgatcattga caaactggaa gaggacataa
      601 gcagctctat gaccaatagc acagctgcca gtagaccccc ggtcaccctg aggctggtgg
      661 tccctgctag tcagtgtggc tctctcattg gaaaaggtgg atgcaagatc aaggaaatac
      721 gagagagtac aggggctcag gtccaggtgg caggggatat gctacccaac tcaactgagc
      781 gggccatcac tattgctggc attccacaat ccatcattga gtgtgtcaaa cagatctgcg
      841 tggtcatgtt ggagtccccc ccgaagggcg tgaccatccc gtaccggccc aagccgtcca
      901 gctctccggt catctttgca ggtggtcagg acaggtacag cacaggcagc gacagtgcga
      961 gctttcccca caccaccccg tccatgtgcc tcaaccctga cctggaggga ccacctctag
     1021 aggcctatac cattcaagga cagtatgcca ttccacagcc agatttgacc aagctgcacc
     1081 agttggcaat gcaacagtct cattttccca tgacgcatgg caacaccgga ttcagtggca
     1141 ttgaatccag ctctccagag gtgaaaggct attgggcagg tttggatgca tctgctcaga
     1201 ctacttctca tgaactcacc attccaaacg atttgattgg ctgcataatc gggcgtcaag
     1261 gcgccaaaat caatgagatc cgtcagatgt ctggggcgca gatcaaaatt gcgaacccag
     1321 tggaaggatc tactgatagg caggttacca tcactggatc tgctgccagc attagcctgg
     1381 ctcaatatct aatcaatgtc aggctttcct cggagacggg tggcatgggg agcagctaga
     1441 acaatgcaga ttcatccata atccctttct gctgttcacc accacccatg atccatctgt
     1501 gtagtttctg aacagtcagc gattccaggt tttaaatagt ttgtaaattt tcagtttcta
     1561 cacactttat catccactcg tgatttttta attaaagcgt tttaattcct ttctctgttc
     1621 agctgttgat gctgagatcc atatttagtt ttataagctt ctccctggtt tttttttttt
     1681 ggctcatgaa tttttctgtt tgtcatggaa atgtaagagt ggaatattaa tacatttcag
     1741 tttagttctg taatgtcagg aatttttcaa aaaaattaaa agatggactg gagctttttc
     1801 tttgtgaata gaaactggat gccacagtga ttcatgtggg ttttattcct cttgtcttgc
     1861 tgttattttt gtacctttta tccctcaaag gacccttctt gggttttgaa tggaagcctt
     1921 tattccggtt aagatgtttt cttctatttt accacttcca tctttttttg tggccctcga
     1981 tcctattttt ccctgactcc atgcttggtt ggcccttata aaacttgtgc ccaaaagatt
     2041 gaggattaga ctttccgagg acttacctgt cctaggggag taggcaagca cttccactag
     2101 ggagggggtg ggggaaagga atgacacatg acatacatgg catacacatt aagcagttga
     2161 tcatatgtct gactgggttc cagtttcttg ggaatgttgg tccccttgtt caggcttgca
     2221 tattttaaac taaaaatttc agtctattgt ttttagtaac ttcatttata gtcctccata
     2281 acaagttaga aggatgtatc tgctaccatt tattcctata attttagaaa gttggggctt
     2341 gacattatac tcatttagtg agagtagatg caaaaaagtg gaggggcagg agaacttctc
     2401 cagacacctc agataaagtc cggagcccaa ggctttatct taaccatgta tggtacccca
     2461 ttcattcatc aagaaaaccc tcaacagctg ggcctgcatg gagtgttata tttcaaggtt
     2521 tttcacaggg gttacagtag gacagtcccc accccaatca ggcaccagga taaaagcagg
     2581 gacttaaaca gcaccccggt tcttcagcct gagccatcac atgctatcag tctcctaacc
     2641 tccccctggg ccttaagaca gggcttgggc agagaagata aatggtggga caaaaaaatg
     2701 agttacattg ccacctgaga aacctcagag gggaggaccc agccttagcc tccctcctcc
     2761 caagtgcaaa atgtgtaaac agagtaaacg gaacagaaaa gtgcagtcta agtggttttc
     2821 tctcctgccc ctcccaccgc ccctcccccc accccctatt atttggggat aaagaatata
     2881 aagacaaccc tggcttttct attgccttgt tgcttgctga atataaggaa tggggtgggg
     2941 caggaagggg cttgccctta gccacagctc tacggctgtg cctcattcat ttccacagct
     3001 gccagtgtcc ctagagttta tcaggtgaat tggtcagggg atcagtctcc ctcgagcctg
     3061 acttacggct gggacagccc catctttctg ttgattatgt ggcgcatata tatatatata
     3121 tgtatatata tataatttat ataaatattt ctctatgta
//
