LOCUS       NM_001405607            7991 bp    mRNA    linear   PRI 27-MAY-2024
DEFINITION  Homo sapiens polybromo 1 (PBRM1), transcript variant 49, mRNA.
ACCESSION   NM_001405607 XM_005265280
VERSION     NM_001405607.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Bursch,K.L., Goetz,C.J., Jiao,G., Nunez,R., Olp,M.D., Dhiman,A.,
            Khurana,M., Zimmermann,M.T., Urrutia,R.A., Dykhuizen,E.C. and
            Smith,B.C.
  TITLE     Cancer-associated polybromo-1 bromodomain 4 missense variants
            variably impact bromodomain ligand binding and cell growth
            suppression
  JOURNAL   J Biol Chem 300 (4), 107146 (2024)
   PUBMED   38460939
  REMARK    GeneRIF: Cancer-associated polybromo-1 bromodomain 4 missense
            variants variably impact bromodomain ligand binding and cell growth
            suppression.
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Ho,P.J., Kweon,J., Blumensaadt,L.A., Neely,A.E., Kalika,E.,
            Leon,D.B., Oh,S., Stringer,C.W.P., Lloyd,S.M., Ren,Z. and Bao,X.
  TITLE     Multi-omics integration identifies cell-state-specific repression
            by PBRM1-PIAS1 cooperation
  JOURNAL   Cell Genom 4 (1), 100471 (2024)
   PUBMED   38190100
  REMARK    GeneRIF: Multi-omics integration identifies cell-state-specific
            repression by PBRM1-PIAS1 cooperation.
REFERENCE   3  (bases 1 to 7991)
  AUTHORS   Demirel,E. and Dilek,O.
  TITLE     Relationship between body composition and PBRM1 mutations in clear
            cell renal cell carcinoma: a propensity score matching analysis
  JOURNAL   Rev Assoc Med Bras (1992) 69 (5), e20220415 (2023)
   PUBMED   37222312
  REMARK    GeneRIF: Relationship between body composition and PBRM1 mutations
            in clear cell renal cell carcinoma: a propensity score matching
            analysis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7991)
  AUTHORS   De Silva,S.M., Dhiman,A., Sood,S., Mercedes,K.F., Simmons,W.J.,
            Henen,M.A., Vogeli,B., Dykhuizen,E.C. and Musselman,C.A.
  TITLE     PBRM1 bromodomains associate with RNA to facilitate chromatin
            association
  JOURNAL   Nucleic Acids Res 51 (8), 3631-3649 (2023)
   PUBMED   36808431
  REMARK    GeneRIF: PBRM1 bromodomains associate with RNA to facilitate
            chromatin association.
REFERENCE   5  (bases 1 to 7991)
  AUTHORS   Yao,X., Hong,J.H., Nargund,A.M., Ng,M.S.W., Heng,H.L., Li,Z.,
            Guan,P., Sugiura,M., Chu,P.L., Wang,L.C., Ye,X., Qu,J., Kwek,X.Y.,
            Lim,J.C.T., Ooi,W.F., Koh,J., Wang,Z., Pan,Y.F., Ong,Y.S.,
            Tan,K.Y., Goh,J.Y., Ng,S.R., Pignata,L., Huang,D., Lezhava,A.,
            Tay,S.T., Lee,M., Yeo,X.H., Tam,W.L., Rha,S.Y., Li,S., Guccione,E.,
            Futreal,A., Tan,J., Yeong,J.P.S., Hong,W., Yauch,R., Chang,K.T.,
            Sobota,R.M., Tan,P. and Teh,B.T.
  TITLE     PBRM1-deficient PBAF complexes target aberrant genomic loci to
            activate the NF-kappaB pathway in clear cell renal cell carcinoma
  JOURNAL   Nat Cell Biol 25 (5), 765-777 (2023)
   PUBMED   37095322
  REMARK    GeneRIF: PBRM1-deficient PBAF complexes target aberrant genomic
            loci to activate the NF-kappaB pathway in clear cell renal cell
            carcinoma.
REFERENCE   6  (bases 1 to 7991)
  AUTHORS   Horikawa,I. and Barrett,J.C.
  TITLE     cDNA cloning of the human polybromo-1 gene on chromosome 3p21
  JOURNAL   DNA Seq 13 (4), 211-215 (2002)
   PUBMED   12487023
  REMARK    GeneRIF: cDNA cloning; the hPB1 gene is located on chromosome 3p21,
            where the tumor suppressor genes for breast, lung and kidney
            cancers have been mapped
REFERENCE   7  (bases 1 to 7991)
  AUTHORS   Phelan,M.L., Sif,S., Narlikar,G.J. and Kingston,R.E.
  TITLE     Reconstitution of a core chromatin remodeling complex from SWI/SNF
            subunits
  JOURNAL   Mol Cell 3 (2), 247-253 (1999)
   PUBMED   10078207
REFERENCE   8  (bases 1 to 7991)
  AUTHORS   Reyes,J.C., Muchardt,C. and Yaniv,M.
  TITLE     Components of the human SWI/SNF complex are enriched in active
            chromatin and are associated with the nuclear matrix
  JOURNAL   J Cell Biol 137 (2), 263-274 (1997)
   PUBMED   9128241
REFERENCE   9  (bases 1 to 7991)
  AUTHORS   Wang,W., Cote,J., Xue,Y., Zhou,S., Khavari,P.A., Biggar,S.R.,
            Muchardt,C., Kalpana,G.V., Goff,S.P., Yaniv,M., Workman,J.L. and
            Crabtree,G.R.
  TITLE     Purification and biochemical heterogeneity of the mammalian SWI-SNF
            complex
  JOURNAL   EMBO J 15 (19), 5370-5382 (1996)
   PUBMED   8895581
REFERENCE   10 (bases 1 to 7991)
  AUTHORS   Wang,W., Xue,Y., Zhou,S., Kuo,A., Cairns,B.R. and Crabtree,G.R.
  TITLE     Diversity and specialization of mammalian SWI/SNF complexes
  JOURNAL   Genes Dev 10 (17), 2117-2130 (1996)
   PUBMED   8804307
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104446.2 and AC112215.3.
            
            On Apr 28, 2022 this sequence version replaced XM_005265280.4.
            
            Summary: This locus encodes a subunit of ATP-dependent
            chromatin-remodeling complexes. The encoded protein has been
            identified as in integral component of complexes necessary for
            ligand-dependent transcriptional activation by nuclear hormone
            receptors. Mutations at this locus have been associated with
            primary clear cell renal cell carcinoma. [provided by RefSeq, Feb
            2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.841145.1,
                                           SRR14038195.706006.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000707071.1/ ENSP00000516722.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC104446.2         138514-138678       c
            166-318             AC104446.2         132339-132491       c
            319-416             AC104446.2         131265-131362       c
            417-564             AC104446.2         121263-121410       c
            565-708             AC104446.2         114898-115041       c
            709-825             AC104446.2         110964-111080       c
            826-894             AC104446.2         104507-104575       c
            895-993             AC112215.3         198260-198358       c
            994-1079            AC112215.3         194620-194705       c
            1080-1175           AC112215.3         193164-193259       c
            1176-1267           AC112215.3         191870-191961       c
            1268-1481           AC112215.3         184518-184731       c
            1482-1623           AC112215.3         178810-178951       c
            1624-1721           AC112215.3         177189-177286       c
            1722-1766           AC112215.3         174813-174857       c
            1767-2043           AC112215.3         167178-167454       c
            2044-2149           AC112215.3         165267-165372       c
            2150-2792           AC112215.3         159229-159871       c
            2793-3004           AC112215.3         153437-153648       c
            3005-3190           AC112215.3         138986-139171       c
            3191-3348           AC112215.3         137269-137426       c
            3349-3612           AC112215.3         136341-136604       c
            3613-3758           AC112215.3         128970-129115       c
            3759-3916           AC112215.3         126457-126614       c
            3917-4100           AC112215.3         113966-114149       c
            4101-4311           AC112215.3         113199-113409       c
            4312-4513           AC112215.3         111683-111884       c
            4514-4678           AC112215.3         108165-108329       c
            4679-4834           AC112215.3         104640-104795       c
            4835-4905           AC112215.3         100663-100733       c
            4906-5122           AC112215.3         100337-100553       c
            5123-7991           AC112215.3         95283-98151         c
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1"
     gene            1..7991
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /note="polybromo 1"
                     /db_xref="GeneID:55193"
                     /db_xref="HGNC:HGNC:30064"
                     /db_xref="MIM:606083"
     exon            1..165
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            166..318
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..5295
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /note="isoform 48 is encoded by transcript variant 49;
                     polybromo-1D; BRG1-associated factor 180"
                     /codon_start=1
                     /product="protein polybromo-1 isoform 48"
                     /protein_id="NP_001392536.1"
                     /db_xref="GeneID:55193"
                     /db_xref="HGNC:HGNC:30064"
                     /db_xref="MIM:606083"
                     /translation="MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTV
                     DPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLK
                     MEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDE
                     DDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYP
                     DYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSI
                     KKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNK
                     RAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDT
                     VRSCRNNQGQLIAEPFYHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLEC
                     DLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTG
                     SAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPS
                     KKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYN
                     DAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYE
                     AVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMV
                     EDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIH
                     NLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQ
                     EHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLH
                     NDVEKERKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSM
                     YHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEK
                     EVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKI
                     KLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEK
                     EDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAA
                     YFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPT
                     EIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLL
                     EKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQS
                     TPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLE
                     TAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQ
                     LTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPG
                     VMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFV
                     APPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWL
                     KSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV"
     exon            319..416
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            417..564
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..708
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            709..825
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..894
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..993
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1079
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1175
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1267
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1481
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1623
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1721
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1766
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..2043
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2149
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2792
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..3004
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3190
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3348
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3349..3612
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3613..3758
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3916
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            3917..4100
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..4311
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4312..4513
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4514..4678
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4679..4834
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4835..4905
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            4906..5122
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            5123..7991
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7851..7856
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /note="hexamer: ATTAAA"
     polyA_site      7872
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
     regulatory      7971..7976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /note="hexamer: AATACA"
     polyA_site      7991
                     /gene="PBRM1"
                     /gene_synonym="BAF180; PB1; RCC; SMARCH1"
                     /note="major polyA site"
ORIGIN      
        1 ggttttgcgg cggcaccggg aggggtaagg gaggtgaggg cggcgggtgc cgaagcgacg
       61 gcagcggccg cggccggagg agcaatagca gcagccgtgg cggccacggg gcggggcgcg
      121 gcggtcggtg accgcggccg gggctgcagg cggcggagcg gctggaagaa gttggattcc
      181 atgggttcca agagaagaag agctacctcc ccttccagca gtgtcagcgg ggactttgat
      241 gatgggcacc attctgtgtc aacaccaggc ccaagcagga aaaggaggag actttccaat
      301 cttccaactg tagatcctat tgccgtgtgc catgaactct ataataccat ccgagactat
      361 aaggatgaac agggcagact tctctgtgag ctcttcatta gggcaccaaa gcgaagaaat
      421 caaccagact attatgaagt ggtttctcag cccattgact tgatgaaaat ccaacagaaa
      481 ctaaaaatgg aagagtatga tgatgttaat ttgctgactg ctgacttcca gcttcttttt
      541 aacaatgcaa agtcctatta taagccagat tctcctgaat ataaagccgc ttgcaaactc
      601 tgggatttgt accttcgaac aagaaatgag tttgttcaga aaggagaagc agatgacgaa
      661 gatgatgatg aagatgggca agacaatcag ggcacagtga ctgaaggatc ttctccagct
      721 tacttgaagg agatcctgga gcagcttctt gaagccatag ttgtagctac aaatccatca
      781 ggacgtctca ttagcgaact ttttcagaaa ctgccttcta aagtgcaata tccagattat
      841 tatgcaataa ttaaggagcc tatagatctc aagaccattg cccagaggat acagaatgga
      901 agctacaaaa gtattcatgc aatggccaaa gatatagatc tcctcgcaaa aaatgccaaa
      961 acttataatg agcctggctc tcaagtattc aaggatgcaa attcaattaa aaaaatattt
     1021 tatatgaaaa aggctgaaat tgaacatcat gaaatggcta agtcaagtct tcgaatgagg
     1081 actccatcca acttggctgc agccagactg acaggtcctt cacacagtaa aggcagcctt
     1141 ggtgaagaga gaaatcccac tagcaagtat taccgtaata aaagagcagt acaaggaggt
     1201 cgtttatcag caattacaat ggcacttcaa tatggctcag aaagtgaaga agatgctgct
     1261 ttagctgctg cacgctatga agagggagag tcagaagcag aaagcatcac ttcctttatg
     1321 gatgtttcaa atccttttta tcagctttat gacacagtta ggagttgtcg gaataaccaa
     1381 gggcagctaa tagctgaacc tttttaccat ttgccttcaa agaaaaaata ccctgattat
     1441 taccagcaaa ttaaaatgcc catatcacta caacagatcc gaacaaaact gaagaatcaa
     1501 gaatatgaaa ctttagatca tttggagtgt gatctgaatt taatgtttga aaatgccaaa
     1561 cgctataatg tgcccaattc agccatctac aagcgagttc taaaattgca gcaagttatg
     1621 caggcaaaga agaaagagct tgccaggaga gacgatatcg aggacggaga cagcatgatc
     1681 tcttcagcca cctctgatac tggtagtgcc aaaagaaaaa ggaacactca tgacagtgag
     1741 atgttgggtc tcaggaggct atccagtaaa aagaacataa gaaagcagcg aatgaaaatc
     1801 ttattcaatg ttgttcttga agctcgagag ccaggttcag gcagaagact ttgtgaccta
     1861 tttatggtta aaccatccaa aaaggactat cctgattatt ataaaatcat cttggagcca
     1921 atggacttga aaataattga gcataacatc cgcaatgaca aatatgctgg tgaagaggga
     1981 atgatagaag acatgaagct gatgttccgg aatgccaggc actataatga ggagggctcc
     2041 caggtttata atgatgcaca tatcctggag aagttactca aggagaaaag gaaagagctg
     2101 ggcccactgc ctgatgatga tgacatggct tctcccaaac tcaagctgag taggaagagt
     2161 ggcatttctc ctaaaaaatc aaaatacatg actccaatgc agcagaaact aaatgaggtc
     2221 tatgaagctg taaagaacta tactgataag aggggtcgcc gcctcagtgc catatttctg
     2281 aggcttccct ctagatctga gttgcctgac tactatctga ctattaaaaa gcccatggac
     2341 atggaaaaaa ttcgaagtca catgatggcc aacaagtacc aagatattga ctctatggtt
     2401 gaggactttg tcatgatgtt taataatgcc tgtacataca atgagccgga gtctttgatc
     2461 tacaaagatg ctcttgttct acacaaagtc ctgcttgaaa cacgcagaga cctggaggga
     2521 gatgaggact ctcatgtccc aaatgtgact ttgctgattc aagagcttat ccacaatctt
     2581 tttgtgtcag tcatgagtca tcaggatgat gagggaagat gctacagcga ttctttagca
     2641 gaaattcctg ctgtggatcc caactttcct aacaaaccac cccttacatt tgacataatt
     2701 aggaagaatg ttgaaaataa tcgctaccgt cggcttgatt tatttcaaga gcatatgttt
     2761 gaagtattgg aacgagcaag aaggatgaat cggacagatt cagaaatata tgaagatgca
     2821 gtagaacttc agcagttttt tattaaaatt cgtgatgaac tctgcaaaaa tggagagatt
     2881 cttctttcac cggcactcag ctataccaca aaacatttgc ataatgatgt ggagaaagag
     2941 agaaaggaaa aattgccaaa agaaatagag gaagataaac taaaacgaga agaagaaaaa
     3001 agagaagctg aaaagagtga agattcctct ggtgctgcag gcctctcagg cttacatcgc
     3061 acatacagcc aggactgtag ctttaaaaac agcatgtacc atgttggaga ttacgtctat
     3121 gtggaacctg cagaggccaa cctacaacca catatcgtct gtattgaaag actgtgggag
     3181 gattcagctg gtgaaaaatg gttgtatggc tgttggtttt accgaccaaa tgaaacattc
     3241 cacctggcta cacgaaaatt tctagaaaaa gaagttttta agagtgacta ttacaacaaa
     3301 gttccagtta gtaaaattct aggcaagtgt gtggtcatgt ttgtcaagga atactttaag
     3361 ttatgcccag aaaacttccg agatgaggat gtttttgtct gtgaatcacg gtattctgcc
     3421 aaaaccaaat cttttaagaa aattaaactg tggaccatgc ccatcagctc agtcaggttt
     3481 gtccctcggg atgtgcctct gcctgtggtt cgcgtggcct ctgtatttgc aaatgcagat
     3541 aaaggtgatg atgagaagaa tacagacaac tcagaggaca gtcgagctga agacaatttt
     3601 aacttggaaa aggaaaaaga agatgtccct gtggaaatgt ccaatggtga accaggttgc
     3661 cactactttg agcagctcca ttacaatgac atgtggctga aggttggcga ctgtgtcttc
     3721 atcaagtccc atggcctggt gcgtcctcgt gtgggcagaa ttgaaaaagt atgggttcga
     3781 gatggagctg catattttta tggccccatc ttcattcacc cagaagaaac agagcatgag
     3841 cccacaaaaa tgttctacaa aaaagaagta tttctgagta atctggaaga aacctgcccc
     3901 atgacatgta ttctcggaaa gtgtgctgtg ttgtcattca aggacttcct ctcctgcagg
     3961 ccaactgaaa taccagaaaa tgacattctg ctttgtgaga gccgctacaa tgagagcgac
     4021 aagcagatga agaaattcaa aggattgaag aggttttcac tctctgctaa agtggtagat
     4081 gatgaaattt actacttcag aaaaccaatt gttcctcaga aggagccatc acctttgctg
     4141 gaaaagaaga tccagttgct agaagctaaa tttgccgagt tagaaggtgg agatgatgat
     4201 attgaagaga tgggagaaga agatagtgag gtcattgaac ctccttctct acctcagctt
     4261 cagacccccc tggccagtga gctggacctc atgccctaca cacccccaca gtctacccca
     4321 aagtctgcca aaggcagtgc aaagaaggaa ggctccaaac ggaaaatcaa catgagtggc
     4381 tacatcctgt tcagcagtga gatgagggct gtgattaagg cccaacaccc agactactct
     4441 ttcggggagc tcagccgcct ggtggggaca gaatggagaa atcttgagac agccaagaaa
     4501 gcagaatatg aagagcgggc agctaaagtt gctgagcagc aggagagaga gcgagcagca
     4561 cagcaacagc agccgagtgc ttctccccga gcaggcaccc ctgtgggggc tctcatgggg
     4621 gtggtgccac caccaacacc aatggggatg ctcaatcagc agttgacacc tgttgcaggc
     4681 atgatgggtg gctatccgcc aggccttcca cctttgcagg gcccagttga tggccttgtt
     4741 agcatgggca gcatgcagcc acttcaccct ggggggcctc caccccacca tcttccgcca
     4801 ggtgtgcctg gcctcccggg catcccacca ccgggtgtga tgaaccaagg agtggcccct
     4861 atggtaggga ctccagcacc aggtggaagt ccatatggac aacaggtggg agttttgggg
     4921 cctccagggc agcaggcacc acctccatat cccggcccac atccagctgg accccctgtc
     4981 atacagcagc caacaacacc catgtttgta gctcccccac caaagaccca gcggcttctt
     5041 cactcagagg cctacctgaa atacattgaa ggactcagtg cggagtccaa cagcattagc
     5101 aagtgggatc agacactggc agctcgaaga cgcgacgtcc atttgtcgaa agaacaggag
     5161 agccgcctac cctctcactg gctgaaaagc aaaggggccc acaccaccat ggcagatgcc
     5221 ctctggcgcc ttcgagattt gatgctccgg gacaccctca acattcgcca agcatacaac
     5281 ctagaaaatg tttaatcaca tcattacgtt tcttttatat agaagcataa agagttgtgg
     5341 atcagtagcc attttagtta ctgggggtgg ggggaaggaa caaaggagga taatttttat
     5401 tgcattttac tgtacatcac aaggccattt ttatatacgg acacttttaa taagctattt
     5461 caatttgttt gttatattaa gttgacttta tcaaatacac aaagattttt ttgcatatgt
     5521 ttccttcgtt taaaaccagt ttcataattg gttgtatatg tagacttgga gttttatctt
     5581 tttacttgtt gccatggaac tgaaaccatt agaggttttt gtcttggctt ggggtttttg
     5641 ttttcttggt tttgggtttt tttatatata tatataaaag aacaaaatga aaaaaaacac
     5701 acacacacaa gagtttacag attagtttaa attgataatg aaatgtgaag tttgtcctag
     5761 tttacatctt agagagggga gtatacttgt gtttgtttca tgtgcctgaa tatcttaagc
     5821 cactttctgc aaaagctgtt tcttacagat gaagtgcttt ctttgaaagg tggttattta
     5881 ggttttagat gtttaataga cacagcacat ttgctctatt aactcagagg ctcactacag
     5941 aaatatgtaa tcagtgctgt gcatctgtct gcagctaatg tacctcctgg acaccaggag
     6001 gggaaaaagc actttttcaa ttgtgctgag ttagacatct gtgagttaga ctatggtgtc
     6061 agtgattttt gcagaacacg tgcacaaccc tgaggtatgt ttaatctagg caggtacgtt
     6121 taaggatatt ttgatctatt tataatgaat tcacaattta tgcctataaa tttcagatga
     6181 tttaaaattt taaacctgtt acattgaaaa acattgaagt tcgtcttgaa gaaagcatta
     6241 aggtatgcat ggaggtgatt tatttttaaa cataacacct aacctaacat gggtaagaga
     6301 gtatggaact agatatgagc tgtataagaa gcataattgt gaacaagtag attgattgcc
     6361 ttcatataca agtatgtttt agtattcctt atttccttat tatcagatgt attttttctt
     6421 ttaagtttca atgttgttat aattctcaac cagaaattta atactttcta aaatattttt
     6481 taaatttagc ttgtgctttt gaattacagg agaagggaat cataatttaa taaaacgctt
     6541 actagaaaga ccattacaga tcccaaacac ttgggtttgg tgaccctgtc tttcttatat
     6601 gaccctacaa taaacatttg aaggcagcat aggatggcag acagtaggaa cattgtttca
     6661 cttggcggca tgtttttgaa acctgcttta tagtaactgg gtgattgcca ttgtggtaga
     6721 gcttccactg ctgtttataa tctgagagag ttaatctcag aggatgcttt tttcctttta
     6781 atctgctatg aatcagtacc cagatgttta attactgtac ttattaaatc atgagggcaa
     6841 aagagtgtag aatggaaaaa agtctcttgt atctagatac tttaaatatg ggaggccctt
     6901 taacttaatt gcctttagtc aaccactgga tttgaatttg catcaagtat tttaaataat
     6961 attgaattta aaaaaatgta ttgcagtagt gtgtcagtac cttattgtta aagtgagtca
     7021 gataaatctt caattcctgg ctatttgggc aattgaatca tcatggactg tataatgcaa
     7081 tcagattatt ttgtttctag acatccttga attacaccaa agaacatgaa atttagttgt
     7141 ggttaaatta tttatttatt tcatgcattc attttatttc ccttaaggtc tggatgagac
     7201 ttctttgggg agcctctaaa aaaatttttc actgggggcc acgtgggtca ttagaagcca
     7261 gagctctcct ccaggctcct tcccagtgcc tagaggtgct ataggaaaca tagatccagc
     7321 caggggcttc cctaaagcag tgcagcaccg gcccagggca tcactagaca ggccctaatt
     7381 aagttttttt taaaaagcct gtgtatttat tttagaatca tgtttttctg tatattaact
     7441 tgggggatat cgttaatatt taggatataa gatttgaggt cagccatctt caaaaaagaa
     7501 aaaaaaattg actcaagaaa gtacaagtaa actatacacc tttttttcat aagttttagg
     7561 aactgtagta atgtggctta gaaagtataa tggcctaaat gttttcaaaa tgtaagttcc
     7621 tgtggagaag aattgtttat attgcaaacg gggggactga ggggaacctg taggtttaaa
     7681 acagtatgtt tgtcagccaa ctgatttaaa aggcctttaa ctgttttggt tgttgttttt
     7741 tttttaagcc actctcccct tcctatgagg aagaattgag aggggcacct atttctgtaa
     7801 aatccccaaa ttggtgttga tgattttgag cttgaatgtt ttcatacctg attaaaactt
     7861 ggtttattct aatttctgta tcatatcatc tgaggtttac gtggtaacta gtcttataac
     7921 atgtatgtat cttttttttg ttgttcatct aaagcttttt aatccaaata aatacagagt
     7981 ttgcaaagtg a
//
