LOCUS       NM_015148               4540 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens PAS domain containing serine/threonine kinase (PASK),
            transcript variant 2, mRNA.
ACCESSION   NM_015148
VERSION     NM_015148.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Karakkat,J.V., Kaimala,S., Sreedharan,S.P., Jayaprakash,P.,
            Adeghate,E.A., Ansari,S.A., Guccione,E., Mensah-Brown,E.P.K. and
            Starling Emerald,B.
  TITLE     The metabolic sensor PASK is a histone 3 kinase that also regulates
            H3K4 methylation by associating with H3K4 MLL2 methyltransferase
            complex
  JOURNAL   Nucleic Acids Res 47 (19), 10086-10103 (2019)
   PUBMED   31529049
  REMARK    GeneRIF: we show that the nuclear PASK associates with the
            mammalian H3K4 MLL2 methyltransferase complex and enhances H3K4 di-
            and tri-methylation. We also show that PASK is a histone kinase
            that phosphorylates H3 at T3, T6, S10 and T11.
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Kikani,C.K., Wu,X., Fogarty,S., Kang,S.A.W., Dephoure,N.,
            Gygi,S.P., Sabatini,D.M. and Rutter,J.
  TITLE     Activation of PASK by mTORC1 is required for the onset of the
            terminal differentiation program
  JOURNAL   Proc Natl Acad Sci U S A 116 (21), 10382-10391 (2019)
   PUBMED   31072927
  REMARK    GeneRIF: MTORC1-PASK signaling is required for the rise of
            myogenin-positive committed myoblasts.
REFERENCE   3  (bases 1 to 4540)
  AUTHORS   DeMille,D., Pape,J.A., Bikman,B.T., Ghassemian,M. and Grose,J.H.
  TITLE     The Regulation of Cbf1 by PAS Kinase Is a Pivotal Control Point for
            Lipogenesis vs. Respiration in Saccharomyces cerevisiae
  JOURNAL   G3 (Bethesda) 9 (1), 33-46 (2019)
   PUBMED   30381292
  REMARK    GeneRIF: we provide the first evidence for a role of USF1 in
            respiration since it appeared to complement Cbf1 in vivo as
            determined by respiration phenotypes. In addition, we confirmed
            USF1 as a substrate of human PAS kinase (hPASK) in vitro Combined,
            our data supports a model in which Cbf1/USF1 functions to partition
            glucose toward respiration and away from lipid biogenesis
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4540)
  AUTHORS   Semache,M., Zarrouki,B., Fontes,G., Fogarty,S., Kikani,C.,
            Chawki,M.B., Rutter,J. and Poitout,V.
  TITLE     Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1
            protein stability via phosphorylation of glycogen synthase kinase
            3beta in pancreatic beta-cells
  JOURNAL   J Biol Chem 288 (34), 24825-24833 (2013)
   PUBMED   23853095
  REMARK    GeneRIF: PASK phosphorylates and inactivates GSK3beta, thereby
            preventing PDX-1 serine phosphorylation and alleviating
            GSK3beta-mediated PDX-1 protein degradation in pancreatic
            beta-cells.
REFERENCE   5  (bases 1 to 4540)
  AUTHORS   da Silva Xavier,G., Farhan,H., Kim,H., Caxaria,S., Johnson,P.,
            Hughes,S., Bugliani,M., Marselli,L., Marchetti,P., Birzele,F.,
            Sun,G., Scharfmann,R., Rutter,J., Siniakowicz,K., Weir,G.,
            Parker,H., Reimann,F., Gribble,F.M. and Rutter,G.A.
  TITLE     Per-arnt-sim (PAS) domain-containing protein kinase is
            downregulated in human islets in type 2 diabetes and regulates
            glucagon secretion
  JOURNAL   Diabetologia 54 (4), 819-827 (2011)
   PUBMED   21181396
  REMARK    GeneRIF: PASK is involved in the regulation of glucagon secretion
            by glucose and may be a useful target for the treatment of type 2
            diabetes.
REFERENCE   6  (bases 1 to 4540)
  AUTHORS   Wilson,W.A., Skurat,A.V., Probst,B., de Paoli-Roach,A., Roach,P.J.
            and Rutter,J.
  TITLE     Control of mammalian glycogen synthase by PAS kinase
  JOURNAL   Proc Natl Acad Sci U S A 102 (46), 16596-16601 (2005)
   PUBMED   16275910
REFERENCE   7  (bases 1 to 4540)
  AUTHORS   da Silva Xavier,G., Rutter,J. and Rutter,G.A.
  TITLE     Involvement of Per-Arnt-Sim (PAS) kinase in the stimulation of
            preproinsulin and pancreatic duodenum homeobox 1 gene expression by
            glucose
  JOURNAL   Proc Natl Acad Sci U S A 101 (22), 8319-8324 (2004)
   PUBMED   15148392
  REMARK    GeneRIF: Results suggest that elevated glucose concentrations
            rapidly increase Per-Arnt-Sim kinase activity in pancreatic islet
            beta cells, followed by up-regulation by glucose of preproinsulin
            and pancreatic duodenum homeobox 1 gene expression.
REFERENCE   8  (bases 1 to 4540)
  AUTHORS   Amezcua,C.A., Harper,S.M., Rutter,J. and Gardner,K.H.
  TITLE     Structure and interactions of PAS kinase N-terminal PAS domain:
            model for intramolecular kinase regulation
  JOURNAL   Structure 10 (10), 1349-1361 (2002)
   PUBMED   12377121
REFERENCE   9  (bases 1 to 4540)
  AUTHORS   Hofer,T., Spielmann,P., Stengel,P., Stier,B., Katschinski,D.M.,
            Desbaillets,I., Gassmann,M. and Wenger,R.H.
  TITLE     Mammalian PASKIN, a PAS-serine/threonine kinase related to
            bacterial oxygen sensors
  JOURNAL   Biochem Biophys Res Commun 288 (4), 757-764 (2001)
   PUBMED   11688972
  REMARK    GeneRIF: molecular cloning, gene expression, and comparison with
            mouse protein
REFERENCE   10 (bases 1 to 4540)
  AUTHORS   Rutter,J., Michnoff,C.H., Harper,S.M., Gardner,K.H. and
            McKnight,S.L.
  TITLE     PAS kinase: an evolutionarily conserved PAS domain-regulated
            serine/threonine kinase
  JOURNAL   Proc Natl Acad Sci U S A 98 (16), 8991-8996 (2001)
   PUBMED   11459942
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA104684.1 and BC063585.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_015148.3.
            
            Summary: This gene encodes a member of the serine/threonine kinase
            family that contains two PAS domains. Expression of this gene is
            regulated by glucose, and the encoded protein plays a role in the
            regulation of insulin gene expression. Downregulation of this gene
            may play a role in type 2 diabetes. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Nov 2011].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region, but maintains the reading frame, compared
            to variant 1. Variants 2 and 3 encode the same isoform (2), which
            is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063585.1, AF387103.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000234040.9/ ENSP00000234040.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                DA104684.1         33-57
            26-4540             BC063585.1         1-4515
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..4540
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /note="PAS domain containing serine/threonine kinase"
                     /db_xref="GeneID:23178"
                     /db_xref="HGNC:HGNC:17270"
                     /db_xref="MIM:607505"
     exon            1..59
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            60..297
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..4073
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2; PAS
                     domain-containing serine/threonine-protein kinase;
                     per-arnt-sim (PAS) domain kinase"
                     /codon_start=1
                     /product="PAS domain-containing serine/threonine-protein
                     kinase isoform 2"
                     /protein_id="NP_055963.2"
                     /db_xref="CCDS:CCDS2545.1"
                     /db_xref="GeneID:23178"
                     /db_xref="HGNC:HGNC:17270"
                     /db_xref="MIM:607505"
                     /translation="MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSA
                     HRHLSRRNGLSRLCQSRTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSEPR
                     GSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAKTTEILVANDKACGLLGYSSQ
                     DLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEKIPVSVWMK
                     RMRQERRLCCVVVLEPVERVSTWVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITD
                     LIPSVQLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSG
                     YRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLIPGFYSYM
                     DLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVP
                     RDEIRKLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKD
                     QQITALGREEPVAIESPGQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCG
                     LCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQLAGGSLLMHCPCYGSEWGLWWRSQ
                     DLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQLSQLSLAGALDVPHAEL
                     VPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVDVNSFS
                     WNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELL
                     LLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPE
                     DTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRR
                     VELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAELTGPSLVEVLRA
                     RPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFI
                     KKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGL
                     DLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLID
                     FGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFC
                     ELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTW
                     EEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRL
                     LTS"
     exon            298..530
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            531..701
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            702..842
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            843..977
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1238
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1407
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1564
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..2820
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..3005
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3173
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3299
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3434
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3634
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3635..3768
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3769..3915
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     exon            3916..4540
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4321..4326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /note="hexamer: ATTAAA"
     polyA_site      4344
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /note="major polyA site"
     regulatory      4512..4517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
                     /note="hexamer: ATTAAA"
     polyA_site      4540
                     /gene="PASK"
                     /gene_synonym="PASKIN; STK37"
ORIGIN      
        1 gcggaagggc tcctttccgt ggtgtgtagc cggcttggcg tgaccctcgc ctgatccagt
       61 tgttagagtt ggaagcttgg cagttggcct cccttcttcc catggaggac gggggcttaa
      121 cagcctttga agaggaccag agatgccttt cccagagcct ccccttgcca gtgtcagcag
      181 agggcccagc tgcacagacc actgctgagc ccagcaggtc gttttcctca gcccacagac
      241 acctgagcag aaggaatggg ctttccagac tctgccagag caggacagcg ctctctgaag
      301 acagatggag ctcctattgt ctatcatcac tggctgccca gaatatttgt acaagtaaac
      361 tgcactgccc tgctgcccct gagcacacgg acccgtccga accgcggggc agtgtgtcct
      421 gctgctccct gctgcgggga ctgtcctcag ggtggtcctc acctctgctt ccggcccctg
      481 tgtgcaaccc taacaaggcc atcttcacgg tggatgccaa gaccacagag atcctggttg
      541 ctaacgacaa agcttgcggg ctcctggggt acagcagcca ggacctgatt ggccagaagc
      601 tcacgcagtt ctttctgagg tcagattctg atgtggtgga ggccctcagc gaggagcaca
      661 tggaggccga cggccacgct gcggtggtgt ttggcacggt ggtggacatc atcagccgta
      721 gtggggagaa gattccagtg tctgtgtgga tgaagaggat gcggcaggag cgccgcctat
      781 gctgcgtggt ggtcctggag cccgtggaga gggtctcgac ctgggtcgct ttccagagcg
      841 atggcaccgt cacgtcatgt gacagtctct ttgctcatct tcacgggtac gtgtctgggg
      901 aggacgtggc tgggcagcat atcacagacc tgatcccttc tgtgcagctc cctccttctg
      961 gccagcacat cccaaagaat ctcaagattc agaggtctgt tggaagagcc agggacggta
     1021 ccaccttccc tctgagctta aagctgaaat cccaacccag cagcgaggag gcgaccaccg
     1081 gtgaggcggc ccctgtgagc ggctaccggg catctgtctg ggtgttctgc accatcagtg
     1141 gcctcatcac cctcctgccg gatgggacca tccacggcat caaccacagc ttcgcgctga
     1201 cactgtttgg ttacggaaag acggagctcc tgggcaagaa tatcactttc ctgattcctg
     1261 gtttctacag ctacatggac cttgcgtaca acagctcatt acagctccca gacctggcca
     1321 gctgcctgga cgtcggcaat gagagtgggt gtggggagag aaccttggac ccgtggcagg
     1381 gccaggaccc agctgagggg ggccaggatc caaggattaa tgtcgtgctt gctggtggcc
     1441 acgttgtgcc ccgagatgag atccggaagc tgatggaaag ccaagacatc ttcaccggga
     1501 ctcagactga gctgattgct ggaggccagc tcctttcctg cctctcacct cagcctgctc
     1561 caggggtgga caatgtccca gaaggaagcc tgccagtgca cggtgaacag gcgctgccca
     1621 aggaccagca aatcactgcc ttggggagag aggaacctgt ggcaatagag agccccggac
     1681 aggatcttct gggagaaagc aggtctgaac cagtggatgt gaagccattt gcttcctgcg
     1741 aagattctga agctccagtc ccagctgagg atgggggcag tgatgctggc atgtgtggcc
     1801 tgtgtcagaa ggcccagcta gagcggatgg gagtcagtgg tcccagcggt tcagaccttt
     1861 gggctggggc tgccgtggcc aagccccagg ccaagggtca gctggcgggg ggcagcctcc
     1921 tgatgcactg cccttgctat gggagtgaat ggggcttgtg gtggcgaagc caggacttgg
     1981 cccccagccc ctctgggatg gcaggcctct cgtttgggac acctactcta gatgagccgt
     2041 ggctgggagt ggaaaacgac cgagaagagc tgcagacctg cttgattaag gagcagctgt
     2101 cccagttgag ccttgcagga gccctggatg tcccccacgc cgaactcgtt ccgacagagt
     2161 gccaggctgt caccgctcct gtgtcgtcct gcgatctggg aggcagagac ctgtgcggtg
     2221 gctgcacggg cagctcctca gcctgctatg ccttggccac ggacctccct gggggcctgg
     2281 aagcagtgga ggcccaggag gttgatgtga attcgttttc ctggaacctc aaggaactct
     2341 ttttcagtga ccagacagac caaacgtcat caaattgttc ctgtgctacg tctgaactca
     2401 gagagacacc ctcttccttg gcagtgggct ccgatccaga tgtaggcagt ctccaggaac
     2461 aggggtcgtg tgtcctggat gacagggagc tgttactact gaccggcacc tgtgttgacc
     2521 ttggccaagg ccgacggttc cgggagagct gtgtgggaca tgatccaaca gaaccgcttg
     2581 aggtttgttt ggtgtcctct gagcattatg cagcaagcga cagagaaagc ccaggacacg
     2641 ttccttccac gttggatgct ggccctgagg acacgtgccc atcagcagag gagccaaggc
     2701 tgaacgtcca ggtcacctcc acgcccgtga tcgtgatgcg cggggctgct ggcctgcagc
     2761 gggagatcca ggagggtgcc tactccggga gctgctacca tcgagatggc ttacggctga
     2821 gtatacagtt tgaggtgagg cgggtggagc tccagggccc cacacctctg ttctgctgct
     2881 ggctggtgaa agacctcctc cacagccaac gcgactcagc cgccaggacc cgcctgttcc
     2941 ttgccagcct gcccggctcc acccactcta ccgctgctga gctcaccgga cccagcctgg
     3001 tggaagtgct cagagccaga ccctggtttg aggagccccc caaggctgtg gaactggagg
     3061 ggttggcggc ctgtgagggc gagtactccc aaaagtacag taccatgagc ccgctgggca
     3121 gtggggcctt cggcttcgtg tggactgctg tggacaagga aaaaaacaag gaggtggtgg
     3181 tgaagtttat taagaaggag aaggtcttgg aggattgttg gattgaggat cccaaacttg
     3241 ggaaagttac tttagagatc gcaattctat ccagggtgga gcacgccaat atcatcaagg
     3301 tattggatat atttgaaaac caagggttct tccagcttgt gatggagaag cacggctccg
     3361 gcctagacct cttcgctttc atcgaccgcc accccaggct ggatgagccc ctggcgagct
     3421 acatcttccg acaactagtg tcagcagtgg gatacctgcg cttgaaggac atcatccacc
     3481 gtgacatcaa ggatgagaac atcgtgatcg ccgaggactt cacaatcaag ctgatagact
     3541 ttggctcggc cgcctacttg gaaaggggaa aattatttta tactttttgt gggaccatcg
     3601 agtactgtgc accggaagtt ctcatgggga atccctacag agggccggag ctggagatgt
     3661 ggtctctggg agtcactctg tacacgctgg tctttgagga gaaccccttc tgtgagctgg
     3721 aggagaccgt ggaggctgcc atacacccgc catacctggt gtccaaagaa ctcatgagcc
     3781 ttgtgtctgg gctgctgcag ccagtccctg agagacgcac caccttggag aagctggtga
     3841 cagacccgtg ggtaacacag cctgtgaatc ttgctgacta tacatgggaa gaggtgtttc
     3901 gagtaaacaa gccagaaagt ggagttctgt ccgctgcgag cctggagatg gggaacagga
     3961 gcctgagtga tgtggcccag gctcaggagc tttgtggggg ccccgttcca ggcgaggctc
     4021 ctaatggcca aggctgtttg catcccgggg atccccgtct gctgaccagc taaacaccaa
     4081 tttcttcctg cttttctcca cttggtttgg aaaatcacac agttttcagg ctccatctgt
     4141 ttggagaaaa tacattctga agcatcccca attcaccttc taaaaactca tgtgcaggtt
     4201 tgataaacac cagaacagaa gacagtgatg ctgtattatt ttagatttat tacatagatt
     4261 tggaattcac ttttttcatg acctagaaaa aaacattcca gtgttcaact gttttatatt
     4321 attaaagggc ttttaatttg tgaacttctg aaggcatgag tgttttctct ttctactttt
     4381 gtatatgtgc atgttttgtt tcctctgact tggtatatgc tcatctgagt gacggatatg
     4441 tgaaatttgt agaactggtt agtcaaatgg ccagactatt tcattaattt atttcctcaa
     4501 atgcttttca aattaaagca cctttgttag taaacagtta
//
