LOCUS       NM_020144               4293 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens poly(A) polymerase beta (PAPOLB), mRNA.
ACCESSION   NM_020144
VERSION     NM_020144.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Le,Y.J., Kim,H., Chung,J.H. and Lee,Y.
  TITLE     Testis-specific expression of an intronless gene encoding a human
            poly(A) polymerase
  JOURNAL   Mol Cells 11 (3), 379-385 (2001)
   PUBMED   11459229
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Lee,Y.J., Lee,Y. and Chung,J.H.
  TITLE     An intronless gene encoding a poly(A) polymerase is specifically
            expressed in testis
  JOURNAL   FEBS Lett 487 (2), 287-292 (2000)
   PUBMED   11150526
REFERENCE   3  (bases 1 to 4293)
  AUTHORS   Weichs an der Glon,C., Ashe,M., Eggermont,J. and Proudfoot,N.J.
  TITLE     Tat-dependent occlusion of the HIV poly(A) site
  JOURNAL   EMBO J 12 (5), 2119-2128 (1993)
   PUBMED   8491200
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092610.1, BC036653.2 and AF218840.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020144.4.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC036653.2, AF218840.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404991.2/ ENSP00000384700.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AC092610.1         117152-117187       c
            37-115              BC036653.2         1-79
            116-2146            AF218840.1         1-2031
            2147-2400           BC036653.2         2111-2364
            2401-2755           AC092610.1         114433-114787       c
            2756-4293           BC036653.2         2719-4256
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..4293
                     /gene="PAPOLB"
                     /gene_synonym="PAPT; TPAP"
                     /note="poly(A) polymerase beta"
                     /db_xref="GeneID:56903"
                     /db_xref="HGNC:HGNC:15970"
                     /db_xref="MIM:607436"
     exon            1..4293
                     /gene="PAPOLB"
                     /gene_synonym="PAPT; TPAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..2134
                     /gene="PAPOLB"
                     /gene_synonym="PAPT; TPAP"
                     /EC_number="2.7.7.19"
                     /note="testis-specific poly(A) polymerase; PAP-beta;
                     polynucleotide adenylyltransferase beta; poly(A)
                     polymerase beta (testis specific)"
                     /codon_start=1
                     /product="poly(A) polymerase beta"
                     /protein_id="NP_064529.4"
                     /db_xref="CCDS:CCDS78202.1"
                     /db_xref="GeneID:56903"
                     /db_xref="HGNC:HGNC:15970"
                     /db_xref="MIM:607436"
                     /translation="MMPFPVTTQGPPQPAPPPNRYGVSSPISLAVPKETDCLLTQRLI
                     ETLRPFGVFEEEEELQRRILVLEKLNNLVKEWIREISESKSLPQSVIENVGGKIFTFG
                     SYRLGVHTKGADIDALCVAPSHVDRSDFFTSFYAKLKLQEEVKDLRAVEEAFVPVIKL
                     CFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNI
                     DNFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAVASTLVRKFFLV
                     FSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSIST
                     RMVMIEEFKQGLAITHEILLSKAEWSKLFEAPSFFQKYKHYIVLLASASTEKQHLEWV
                     GLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDMEEFRTMWVIGLGLKKPDNS
                     EILSIDLTYDIQSFTDTVYRQAVNSKMFEMGMKITAMHLRRKELHQLLPHHVLQDKKA
                     HSTEGRRLTDLNDSSFDLSAGCENSMSVPSSTSTMKTGPLISSSQGRNSPALAVMTAS
                     VANIQATEFSLQQVNTNESSGVALNESIPHAVSQPAISPSPKAMVARVVSSTCLISHP
                     DLQETQQQTYLIL"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAPOLB"
                     /gene_synonym="PAPT; TPAP"
                     /note="hexamer: AATAAA"
     polyA_site      4293
                     /gene="PAPOLB"
                     /gene_synonym="PAPT; TPAP"
                     /note="major polyA site"
ORIGIN      
        1 aggttctgga acgcagccgc tgaggggtct ggcgccatgt tggggctgag gggaaggagg
       61 agaagctcct gaagcggcgg gcgcgcagcg ggagtgggag tggggtcgga ccagggcagc
      121 tagaagccct gcccttccgc ctcagcggat catgacccgg gcggcggccg cgaaggtcgc
      181 ggtggtgggg gacgtgccct ggcggggcgg gcgctgaaag atgatgccgt ttccggtgac
      241 aacccaggga ccaccgcagc cggcgccgcc gccgaatcgc tacggcgtct cctcgcctat
      301 cagtctagcg gtccccaagg agacggactg cctcctcacc cagaggctaa tagaaaccct
      361 caggcccttc ggggtcttcg aagaggaaga ggaactgcag cgcaggattt tagttttgga
      421 aaaattaaat aatctggtaa aggaatggat acgcgaaatc agtgaaagca agagtcttcc
      481 ccagtctgta attgaaaacg ttggaggaaa gatttttacg tttggctctt acagattagg
      541 agtacatacg aaaggcgcag atattgacgc cttgtgcgtt gcaccaagtc atgtggatcg
      601 aagcgacttt ttcacctcat tctatgctaa actgaaacta caggaggaag tgaaagattt
      661 aagggctgtc gaggaggcat ttgtgccagt tatcaaactg tgttttgatg ggatagagat
      721 tgatatttta tttgcaagat tagcactaca gactattcca gaagatttgg acttaagaga
      781 tgacagtcta cttaaaaatt tagacattag atgcataaga agccttaatg gttgccgggt
      841 aaccgatgaa attttacatc tagtgccaaa cattgacaac ttcaggctga ctctgagagc
      901 catcaaactg tgggccaagt gccacaatat ctattccaat atattaggtt tcctcggagg
      961 tgtttcctgg gccatgctag tagcaagaac ttgtcagctt tatccaaatg cagtagcgtc
     1021 aactcttgta cggaaattct tcttggtatt ttcagaatgg gaatggccaa acccagtgtt
     1081 actgaaggag cctgaagaac ggaatcttaa tttgcctgta tgggacccaa gagtaaatcc
     1141 cagtgatagg taccatctta tgcctatcat cacaccagca tacccacagc agaactccac
     1201 atacaacgtg tctatttcaa ccaggatggt catgattgag gagtttaaac aggggcttgc
     1261 tatcacacac gagattttgc taagtaaggc agagtggtcc aaactctttg aagctccaag
     1321 cttctttcaa aagtacaagc attatattgt acttctggca agtgcatcaa cagaaaaaca
     1381 acatttagaa tgggtgggct tggtggaatc aaagatccga atcctggttg ggagcttgga
     1441 gaagaatgaa tttattacac tggcacatgt gaatccacag tcatttccag cacccaaaga
     1501 aaatcctgat atggaagaat ttcgtacaat gtgggtgatt gggttagggc taaaaaagcc
     1561 agataattct gaaattctca gcattgatct cacctatgat atccagtctt tcacagatac
     1621 tgtttatagg caagcagtga atagtaagat gtttgagatg ggtatgaaaa ttactgcaat
     1681 gcatttaaga agaaaggaac ttcaccagct gctgcctcat catgtgcttc aggacaagaa
     1741 agcacactca acagaaggta gaagattgac agatttgaac gacagcagct ttgacttgtc
     1801 tgcaggctgt gaaaacagca tgtctgtgcc ttcatctact agcactatga agacaggccc
     1861 attgattagc agttctcagg gtagaaacag tcctgccttg gctgtaatga cagcatctgt
     1921 ggctaacata caggctactg aattttcctt gcaacaggtg aataccaatg aaagttcagg
     1981 ggttgcatta aacgaaagta ttcctcacgc tgtctctcag cctgccattt ctccatcacc
     2041 aaaggccatg gtcgccagag ttgtttcttc aacatgtctc ataagccatc cagaccttca
     2101 ggaaactcag caacaaacat atctaatcct atagttggag tctagaggat attcttgcct
     2161 cataaagaag aaaggaccaa gaaaaccaaa acggaagagg agaaactcaa tacaaagaca
     2221 agtacaactc cattagaaaa attcagacag catcttctct attggcctct cagggaacat
     2281 cagtatagat ctttccggta tccctgttct ccctgcaaat ccaattcctg ttatcaagaa
     2341 ttcagtaaaa caggttgaat ggttaaaaac aacctcaagg gtccatgaac aatatctgcc
     2401 aactgaacct gttgtcttca aatactaagg aaggagaatg aggggtacgg gactagatat
     2461 gacttgaaat agattgaggc tcatttggta acttccttta ctgagccagt cttgatcaga
     2521 agtccacatt agtatgtgac agctagaggt actgttatta atgtatcagc aacagttaca
     2581 ttaactattt caaaggacta ctgcccttaa aatgaaaaaa cctttcagct atgtttgtac
     2641 ccgtgactga catctggact ggatttatgc ttgatgcatt gttgggaaaa tatgcaatac
     2701 aaactggtat cagaattcct tatactgatg aggcacttta tattttttat tagaaagtag
     2761 aactaatttt agatttttca aattggtgaa ttttattttt ccctgaagag gtttcttcac
     2821 tattaatctt aaaattggat accattgtgc agcggtgtac tgtacttcag agaaagcata
     2881 aaagtacatc tagctcagtc cctatgaggt agctgtaacc ctttaaaatg aaatgtcaac
     2941 tttaaggtat gtttttgaca tcaaccatta gtaggtcttt ggttttgatg gcacactatg
     3001 gagaaaggac acactggttt taatctgtag aactgtttta gagtaattag caatcaaaca
     3061 ttttctttga gctatcaaag ctccatattc tcttgtcctc aatcattagt cctaaaacaa
     3121 aaaagctttt tgcctccctc tcttctcttt gcctgtatgc acacagtatg cttatgtgtc
     3181 atttcacaag aaaaaaggaa taatattctc taaaccatga tgtgaaactg acaatcctgt
     3241 ggccacatgg cacaaaacac caaatattgg ttatgatgaa acaccacatt ttagcaaaac
     3301 tttactaaat gtaatgtcta tgagatatgt ttctgatctt ggatgaccat ttgatcactg
     3361 catatgagaa gttttgttca ttccacagaa tacctcctta taggaaagtt aatttccaat
     3421 tgcacacata agcaaaaagg cattttcttc aagaatttta aacactttta tttttaaacg
     3481 atttatcaaa attcatctct aaattttaca tgtagaaaca taaaaaagta atttctagca
     3541 agcacttgac atcttactgg ctttctgctc ttttagtcta caaaagatca gcaatacttt
     3601 aagttaaaaa aaatctcata attaagcagt agagcatcta tgattaggaa atgtgagggg
     3661 caacagatgt gaatgaatga aagttgagtc tttcttaatc tagacagcaa acttaacagg
     3721 ctttccaatt agccttgctt taacccctcc cgtccctcct ctaactctgc acttttcatg
     3781 ttaaactgca attacttaaa ctcttttcct gtcctgcatt aattttccaa agttggaata
     3841 tagtcatgtc cttttatgct gtagagactt ttctttcacc attactttct gatatctagt
     3901 acacagtatt tctttttctc acatctttgc cgtttcacca caataataaa tcgggtgaag
     3961 tgtgcacaat atctgtattt tgaatggcta tggcgttagg ttgaaatggc caggcacaaa
     4021 tttagtctgg tcattttgtt tacacattgg gaaaaaaatt agtttattaa acattacctg
     4081 taactgtgtc tgttttacca agctggaacc ttgggacttt ttatttctaa tttttattac
     4141 ctgaatgtca gaccattttt tcatgtgatg tacctagtga gtggagtttg ctttgtttta
     4201 tggtatctgt actcctggta tattcaagca ctgttttgaa aacagatgac ttatttctcc
     4261 attaaaaatg caataaaaat aaatggcaaa caa
//
