LOCUS       NM_001386393            8020 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens pantothenate kinase 2 (PANK2), transcript variant 11,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001386393
VERSION     NM_001386393.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Saini,A., Holla,V.V., Kalikavil Puthanveedu,D., Mehta,S.,
            Elavarasi,A., Pillai,K.S., Mohapatra,P., Kumari,R., Bari,S.,
            Singh,I., Cherian,A., Krishnan,S., Radhakrishnan,D.M., Agarwal,A.,
            Garg,D., Garg,K., Singh,M., Garg,A., Muthusamy,B., Lal,V.,
            Kishore,A., Pal,P.K., Srivastava,A., Faruq,M. and Rajan,R.
  TITLE     Novel PANK2 Variant in Asian Indians with Atypical Pantothenate
            Kinase Associated Neurodegeneration
  JOURNAL   Mov Disord 39 (5), 920-923 (2024)
   PUBMED   38506547
  REMARK    GeneRIF: Novel PANK2 Variant in Asian Indians with Atypical
            Pantothenate Kinase Associated Neurodegeneration.
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Hayflick,S.J.
  TITLE     Defective pantothenate metabolism and neurodegeneration
  JOURNAL   Biochem Soc Trans 42 (4), 1063-1068 (2014)
   PUBMED   25110003
  REMARK    Review article
REFERENCE   3  (bases 1 to 8020)
  AUTHORS   Polster,B.J., Yoon,M.Y. and Hayflick,S.J.
  TITLE     Characterization of the human PANK2 promoter
  JOURNAL   Gene 465 (1-2), 53-60 (2010)
   PUBMED   20603201
  REMARK    GeneRIF: findings validate expression of the short PANK2 isoform
            and enable predictions about potentially deleterious sequence
            variants in the regulatory region of this human disease gene
REFERENCE   4  (bases 1 to 8020)
  AUTHORS   Kotzbauer,P.T., Truax,A.C., Trojanowski,J.Q. and Lee,V.M.
  TITLE     Altered neuronal mitochondrial coenzyme A synthesis in
            neurodegeneration with brain iron accumulation caused by abnormal
            processing, stability, and catalytic activity of mutant
            pantothenate kinase 2
  JOURNAL   J Neurosci 25 (3), 689-698 (2005)
   PUBMED   15659606
  REMARK    GeneRIF: These results suggest that neurodegeneration with brain
            iron accumulation (NBIA; formerly Hallervorden-Spatz disease) is
            caused by altered neuronal mitochondrial lipid metabolism caused by
            mutations disrupting PanK2 protein levels and catalytic activity.
            GeneRIF: Demonstrated that the mitochondrial isoform is
            sequentially cleaved at two sites by the mitochondrial processing
            peptidase, generating a long-lived 48 kDa mature protein localized
            to mitochondria of neurons in human brain.
REFERENCE   5  (bases 1 to 8020)
  AUTHORS   Johnson,M.A., Kuo,Y.M., Westaway,S.K., Parker,S.M., Ching,K.H.,
            Gitschier,J. and Hayflick,S.J.
  TITLE     Mitochondrial localization of human PANK2 and hypotheses of
            secondary iron accumulation in pantothenate kinase-associated
            neurodegeneration
  JOURNAL   Ann N Y Acad Sci 1012, 282-298 (2004)
   PUBMED   15105273
  REMARK    GeneRIF: An unconventional translational start codon, CUG, which is
            polymorphic in the general population is proposed. PANK2 is
            predicted to localize to mitochondria, with a 29 amino acid
            mitochondrial targeting sequence identified.
REFERENCE   6  (bases 1 to 8020)
  AUTHORS   Zhou,B., Westaway,S.K., Levinson,B., Johnson,M.A., Gitschier,J. and
            Hayflick,S.J.
  TITLE     A novel pantothenate kinase gene (PANK2) is defective in
            Hallervorden-Spatz syndrome
  JOURNAL   Nat Genet 28 (4), 345-349 (2001)
   PUBMED   11479594
REFERENCE   7  (bases 1 to 8020)
  AUTHORS   Gregory,A. and Hayflick,S.
  TITLE     Neurodegeneration with Brain Iron Accumulation Disorders Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23447832
REFERENCE   8  (bases 1 to 8020)
  AUTHORS   Gregory,A. and Hayflick,S.J.
  TITLE     Pantothenate Kinase-Associated Neurodegeneration
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301663
REFERENCE   9  (bases 1 to 8020)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 8020)
  AUTHORS   Robishaw,J.D. and Neely,J.R.
  TITLE     Coenzyme A metabolism
  JOURNAL   Am J Physiol 248 (1 Pt 1), E1-E9 (1985)
   PUBMED   2981478
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353194.13 and AL031670.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a protein belonging to the pantothenate
            kinase family and is the only member of that family to be expressed
            in mitochondria. Pantothenate kinase is a key regulatory enzyme in
            the biosynthesis of coenzyme A (CoA) in bacteria and mammalian
            cells. It catalyzes the first committed step in the universal
            biosynthetic pathway leading to CoA and is itself subject to
            regulation through feedback inhibition by acyl CoA species.
            Mutations in this gene are associated with HARP syndrome and
            pantothenate kinase-associated neurodegeneration (PKAN), formerly
            Hallervorden-Spatz syndrome. Alternative splicing, involving the
            use of alternate first exons, results in multiple transcripts
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (11) uses a downstream
            transcription start site and a downstream non-AUG translation start
            site, compared to variant 1. The encoded isoform (7) is also
            mitochondrially localized but has a shorter N-terminus, compared to
            isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.145483.1,
                                           SRR14038191.1793347.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000610179.7/
                                                  ENSP00000477429.2
            non-AUG initiation codon           :: PMID: 11479594, 15105273,
                                                  20603201
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-342               AL353194.13        28253-28594
            343-695             AL353194.13        46792-47144
            696-949             AL353194.13        49443-49696
            950-1126            AL353194.13        51324-51500
            1127-1250           AL353194.13        55793-55916
            1251-1376           AL031670.6         129072-129197       c
            1377-8020           AL031670.6         117981-124624       c
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..8020
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="pantothenate kinase 2"
                     /db_xref="GeneID:80025"
                     /db_xref="HGNC:HGNC:15894"
                     /db_xref="MIM:606157"
     exon            1..342
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..1427
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /EC_number="2.7.1.33"
                     /note="isoform 7 precursor is encoded by transcript
                     variant 11; non-AUG (CUG) translation initiation codon;
                     Hallervorden-Spatz syndrome; pantothenate kinase 2,
                     mitochondrial; pantothenic acid kinase 2; pantothenate
                     kinase-associated neurodegeneration"
                     /codon_start=1
                     /product="pantothenate kinase 2, mitochondrial isoform 7
                     precursor"
                     /protein_id="NP_001373322.1"
                     /db_xref="CCDS:CCDS93004.1"
                     /db_xref="GeneID:80025"
                     /db_xref="HGNC:HGNC:15894"
                     /db_xref="MIM:606157"
                     /translation="MGGLLGRQRLLLRMGGGRLGAPMERHGRASATSVSSAGEQAAGD
                     PEGRRQEPLRRRASSASVPAVGASAEGTRRDRLGSYSGPTSVSRQRVESLRKKRPLFP
                     WFGLDIGGTLVKLVYFEPKDITAEEEEEEVESLKSIRKYLTSNVAYGSTGIRDVHLEL
                     KDLTLCGRKGNLHFIRFPTHDMPAFIQMGRDKNFSSLHTVFCATGGGAYKFEQDFLTI
                     GDLQLCKLDELDCLIKGILYIDSVGFNGRSQCYYFENPADSEKCQKLPFDLKNPYPLL
                     LVNIGSGVSILAVYSKDNYKRVTGTSLGGGTFFGLCCLLTGCTTFEEALEMASRGDST
                     KVDKLVRDIYGGDYERFGLPGWAVASSFGNMMSKEKREAVSKEDLARATLITITNNIG
                     SIARMCALNENINQVVFVGNFLRINTIAMRLLAYALDYWSKGQLKALFSEHEGYFGAV
                     GALLELLKIP"
     exon            343..695
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     exon            696..949
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1126
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1250
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1376
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..8020
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1931
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="major polyA site"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="hexamer: AATGAA"
     polyA_site      2024
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
     regulatory      7994..7999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="hexamer: AATAAA"
     regulatory      7999..8004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="hexamer: AATGAA"
     polyA_site      8015
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
     polyA_site      8020
                     /gene="PANK2"
                     /gene_synonym="C20orf48; HARP; HSS; NBIA1; PKAN"
                     /note="major polyA site"
ORIGIN      
        1 gctctggctg gactgccgcg gaggaggcga gaaggaatcc gacgctgggg ggcttgctcg
       61 ggcggcagcg actgctgctg cggatgggag ggggccggct cggcgcgccc atggagcgcc
      121 acggcagggc ttccgccacc tccgtctcgt cggctgggga gcaggcggcc ggggaccccg
      181 aagggcggcg gcaggagcca ctgcggcgcc gggcgagcag cgcgtcggtg cccgcggtcg
      241 gggcctcggc tgagggcacg aggcgggatc gactgggctc ttacagcggc cccacctcgg
      301 tctcccgcca gcgcgtcgaa agcctgagga aaaagcggcc gctttttcca tggtttggac
      361 tggatatcgg tggaactctg gtcaagctgg tatattttga acccaaagac atcactgctg
      421 aagaagaaga ggaagaagtg gaaagtctta aaagcattcg gaagtacctg acctccaatg
      481 tggcttatgg gtctacaggc attcgggacg tgcacctcga gctgaaggac ctgactctgt
      541 gtggacgcaa aggcaatctg cactttatac gctttcccac tcatgacatg cctgctttta
      601 ttcaaatggg cagagataaa aacttctcga gtctccacac tgtcttttgt gccactggag
      661 gtggagcgta caaatttgag caggattttc tcacaatagg tgatcttcag ctttgcaaac
      721 tggatgaact agattgcttg atcaaaggaa ttttatacat tgactcagtc ggattcaatg
      781 gacggtcaca gtgctattac tttgaaaacc ctgctgattc tgaaaagtgt cagaagttac
      841 catttgattt gaaaaatccg tatcctctgc ttctggtgaa cattggctca ggggttagca
      901 tcttagcagt atattccaaa gataattaca aacgggtcac aggtactagt cttggaggag
      961 gaactttttt tggtctctgc tgtcttctta ctggctgtac cacttttgaa gaagctcttg
     1021 aaatggcatc tcgtggagat agcaccaaag tggataaact agtacgagat atttatggag
     1081 gggactatga gaggtttgga ctgccaggct gggctgtggc ttcaagcttt ggaaacatga
     1141 tgagcaagga gaagcgagag gctgtcagta aagaggacct ggccagagcg actttgatca
     1201 ccatcaccaa caacattggc tcaatagcaa gaatgtgtgc ccttaatgaa aacattaacc
     1261 aggtggtatt tgttggaaat ttcttgagaa ttaatacgat cgccatgcgg cttttggcat
     1321 atgctttgga ttattggtcc aaggggcagt tgaaagcact tttttcggaa cacgagggtt
     1381 attttggagc tgttggagca ctccttgagc tgttgaagat cccgtgatca ttacctgggg
     1441 aggggttcct gaaaccttcc acaatgggat ctgtggactt tcattttttt aagagactta
     1501 ctcaatttca tgactgtact acctgaaaca aagtgagaaa ggacaggtgt atttttctaa
     1561 gtcatcaaga taaatcctta agaattcagt ctaaattagc aaccaggaag gaaaaatata
     1621 ttaaaaacaa caaaaaagtg gcacatgtcc aggcagtgtg aggatttgct gtatataagt
     1681 tgcctgcttt gtatttttga aatctctgca tcactcattg gaagtgcttc tgaagagagc
     1741 tgctctgtgt tcagttgact ggttttgtgt cctgtttgaa cttgctgaat gtaaggcagg
     1801 ctactatgcg ttataatcta atcacaattt gtcaatatgg tcttggcaat catctgtgca
     1861 ttactctggt ttgcattaag cctgtgtgtg aacttactgt aaaacatgtt ttatttcaag
     1921 gttctgcaaa attaattggg caggttaatt gtgtacctga aacttaacaa gcagtttttg
     1981 gaagggcagt tccacgttac tttacactaa atcctgggaa tgaatgtctg gagagacagg
     2041 gcttcttttc ttggcagaat ttccttgggt agtgggcgga ctgttctgat gcaagtgggt
     2101 acactggcag aagcagcaca atctggagga ctaacatgag tcagcagggc cactagaatt
     2161 gacaccccct caggggatca gggagaatct ggtttttctt tcaggctaag tagaaaagga
     2221 agaactgaag tgtctgtcaa agaactcatg gggagtgggg ggtccttcag ggcactgtgg
     2281 cacttgggag gtgagtctca gaagactgcc tgaggagagt gccaggctgc aggtgctctt
     2341 ggtaggggct gaagcttttc tttctgcttt gcagaataat ttcaaaagcc tttcccaagg
     2401 agagaatgca ccttttatat ctgaggtctt aagtggtgcc catattgctg ttgaaggcaa
     2461 ggatgggcct gtacaaagca gccttggacc aggagggaaa accccaaggc tctgcagcct
     2521 ccctgctccc ctccgtttaa ggctgtggtc agggagggat gggcagggtg ccagccgcat
     2581 ggaggcagca gagacacccg tgttccagct ggggcaaggg gaactgatat gtgggctgtg
     2641 ggctgttcca ctaggcgtgg ccacctctgc acatgggcct tggcacttag gaaatatggg
     2701 tgctgctgca ttgggacctt gtgtgggtgc agagcaggat ggctgctgcg tggagggtaa
     2761 ttttgtcccc tggagagtga ttggtgaccc tcttccctgc tgctgctgtt tgcaggctcc
     2821 tggcctatac tacctctgac gccctgatcc tgagttcctc tgccctcctc tgctgccagc
     2881 agcgctgttg gtggcctcac cctcctccag tacccaggca ccaaagatgg caccaccatc
     2941 ctcctgcttg taccacaggg tgctgctccc tgggccaact cctgtgaact tgctcacttc
     3001 ccatggagac cgaggccagg gcctgcccct ctgaggcccc tgttttgtgg tcctgctgcc
     3061 tctgctgtgc tgcgttcagg aggttgtcca ctgctcgacg tgggtcacca tgcagccctt
     3121 gctgttccca caatttaaca gaaaaggaaa ctgaacggat gagtaaggct gcccagggtc
     3181 actcagctgg aaaagagtag agctggactc aaacccggga ctgtctgtct ctggtgcctg
     3241 agttgggaag acgctgttga tgtccccttc tgcctcgctt tgagcttcct cctccgtggg
     3301 ccctccccct actctagaga aactagtcca ttccctcatg ttccaccttg tgctcagtaa
     3361 accctgtcac tccacagcag ggtttttgag acagtctcgc tgtgtcaccc aggtggagtg
     3421 cagtggggcg atctcagctc actgcaacct ctgccttcca ggctcaagca attctcctgc
     3481 cttagcctcc tgcgtagctg agactacagg catgtgccac catggctggc taatttttat
     3541 atttttagta gagatggggt ttcaccatga tggccaggct ggtctcgaac tccagacccc
     3601 aggtgatccg cctgccttgg cctcccaaag tgctgggaat acaggtgtga gcccctgcgc
     3661 ccggctcaca gcagggttct tgataggcca ccagtgaatc ccatgccacc gaatctggca
     3721 ttcatttcac agcctcatct cagttctcac tcagcagagc cctcttgcct gtcctgccac
     3781 ccaggctact aatgttctgg ttcattctct gctgacagtt agctgtggct gtcctcagcc
     3841 tcagtcctgg gtctccccaa gcagtcatta gcatcacctt gactgatttc cagggaacac
     3901 ttcaaccacc ccttacctct gagctccagc cccgagacac tgccttctct ctgcatttgt
     3961 tgatctctgt gcagcagtgc ccacatcctc atcccagctt actgccgtct caccaccttc
     4021 aggttgtgag gtgcccataa actctgctgt taccattgcc acttccctgt cttagcagct
     4081 gtccctttca gtctgccctc cactagtggc catgaagggg gctgtgaccg tggtccaggt
     4141 gagaaaggaa ggcctgaact aagatgggag gtgggtggcc agaatggaga ggggagaagt
     4201 ggatggccag aatggagaga tgcttagagg gctgtgttgg gaagtggtga ctggatacag
     4261 gggagggagg agagatacaa gagggcacct gggctcattt gtggactacg tgattttgag
     4321 actccaaata agcactgggt atttgggttg gagatacaga gctgagaatg atatgcatgt
     4381 aggtgggcaa agcaaggggg atttcatgag ttctaaggta gggtgagaaa aataaccgag
     4441 agttgagcag tgacggagga agctgcaaag aaaacgactg ctcagggaga ggctggccag
     4501 gggcctgtgg ctcaggggac tgagaagagg agctctctag gcatctgaat tgaggttgcc
     4561 cacaattggt tgcaagatcg tggctggcct tgatggaagc tgggaagcca ctcggtccta
     4621 gtgaggggtg gtgaggagag cagcaacggc cacacaggaa ggctttggac gttttttctc
     4681 ctgacactag ttatttccag gcctgggata tagctagggg cttggggtgg gggcaactct
     4741 ttgcagggtg gtgtggcctg tggaggaaac aggctcagtt ccactacttt ggccaggtgc
     4801 aatggctcat gcctgtaatc ccagcacttt ggaaggccaa ggcgggtgga tcacctgagg
     4861 tcaggagttc aagaccagcc tggacaacat ggtgaaaccc cgtctctact gaaaatacaa
     4921 aaataattag ccgggaatgg tggtgcgtgc ctgtaatccc aggtactcga gaggctgagg
     4981 cagacagttg cttgaacctg ggaggcagag gttgctgcag tgagccgaga ttgctgcact
     5041 gcactccagc ctgggtgaca gcaagactgt ctcaaaaaaa aaaaaaaaaa aaaatccgct
     5101 atttcaggtc accctggtgc tggcgttcac gtggaagact gcttatctgt atccagctgt
     5161 ttgcagagct cagggaggcc agggggcttc agactccact gtactctgca ttccaggatt
     5221 ctctgttctc tggctgtggt ggcctgacac actcttcacc actgacccca cccccgcttg
     5281 cacactatgc cacaggaagg gcaggggctt ccatcacaat cactcagtac tagttaccag
     5341 ctctgataga attactcacc ttgagtttcc aagtcttgct gaaggtgctt gcgtaagtgg
     5401 cacatggatg ttccctctgt tcaaaggcat atgcacaaca ctatttttca aaatttgctt
     5461 ttatttacac gtttgagtca attacatgca tgttttattt ttgaggagtg attagcaaaa
     5521 tgcattaaaa ttttaaaata gcaattaaat atacaaaaat atagcttaca aaaaactgcg
     5581 aatgtttaaa aatattctgc tgcagaattt ttagtgtaca aagacgtcta tgaaacctga
     5641 ggttcagcat ttcataaacc tttttttgag ggagttacac aatacctagt gaggaaaccc
     5701 gagggccaaa ctacaagacg aaaaagtgcc ccgttcctgg caatgatgtc agaagaccaa
     5761 gaggcgatag ttaccagcaa cactctagga agaccagctg tcaccagaag ggagttccta
     5821 acacttgcca cacctgctcc cccgaccggg ggcctgggag ggaagggcag atggagagga
     5881 acggtgtgca gtgggcgtga gatgcagatc caccaggctt gggactggca aagggagctg
     5941 agaagagagt tcccccaaag cacaaacaag tctgctgaca ctggtggggc ccttacagtc
     6001 acagaagtaa ggactggatg gtaagccaaa ggtgctgatg cttggtcaaa aagtggagta
     6061 catctttgca tcttgtaaca atttgggctc tacagctgaa ttggcaaagt aaggtttact
     6121 acacaagccc ctctttttct tctgatatcc ccactcaaat ggaagcacac tccccagcac
     6181 atggggatcc cttattaatc tttactaaag aaccgtgaac cagcattttc cagttatcaa
     6241 ctggatattt aggcatgaat ctggacatgt ccatctgctt aagaaatctg cttaagaaat
     6301 ctgtaacagc tacaggaaaa gacacaccca gggccagaga ttctacaaat gccagaagag
     6361 tggatgtgat agagaatttt ttctcaagtt ttgggttagg gcaccagttg caattttaat
     6421 ccaggggagg atctgggctg ctgctgtagg gggcaagtac accagggagg tgggtgtgga
     6481 cagaggcact gggcagctgc tgggaagcga ggcacaagtc tgtgccccta ctcccagggc
     6541 tgcctggagg aagcagctct aactttcatc agcggggaca aaaatgaact taaactaaaa
     6601 aaattattgg ccgggcgcag tggctcacgc ctgtaatccc agcactttgg gaggtcaagg
     6661 caggtgcatc acgaggtcag gagatcgaga ccatcctggc taacacggtg aaactccgtc
     6721 tgtactaaaa atacaaaaaa ttagccgagc gtggtggcgg gcacctgttg tcccagctcc
     6781 cgaggcggga gaatggcgtg aacctgggag gcggagcttg cagtgggcca agatcgtgcc
     6841 actgcattcc agcctgggtg acagagcgag actccgtctc aaaaaaaaaa aaaaaaaaaa
     6901 aaaaaaaaaa aattgttggg gtaatttctg ttgtcaaatg gaaaacaagg cataaaggga
     6961 aaattctgta ggaagcattt tcttttcttg tgtgtttttt tgagacggag tctcgctctg
     7021 ttgcccaggc tggagtgcag tggggatctt ggctcactgc aacttctgtc tcccgggttc
     7081 aatcaattct cctgtcccag cctcctgagt ggctgggatt acaggcatgc gtcaccacac
     7141 ccggcaaatt tttgtatttt tagtagaggg ggtttcacca tattggtcag gctggtcttg
     7201 aactcttgac ctaaggtgat ccgcctgcct cggcctccca aagtgctggg atgttgggat
     7261 tacaggcgtg agccatcgcg cccgaccaga agcattttct tttcaaagtg tgtgcacaga
     7321 gtgaggaggc cagcctgggc aacatagtga gttgagacct gtctctacaa aaaaataatt
     7381 acgaaaatta gccaggtgtg ctggtgtgca cctgtagacg cagctacaga ctgaggcggg
     7441 aggatcactt gagtccagga agttgaggct gcagtgagct acaatggtgc cactgcactc
     7501 cagtctgggt aacaagaccc tgtctcaaca acaacgaaaa gttgggaggg aaagggagta
     7561 gctccatgaa ctgacgctgg acattcaggc atatccacat gagactcact gtgcacttgc
     7621 tcagtagcta agatgtctgc cccacaaaat agaagccttc agttgctgct gctcaaactc
     7681 ctgcatcaaa gacacaagag cttgcctaag tgaccacaac agttgcagcc acacatcacc
     7741 actggtcctc acttggaact ggccaaggta ggggaggggc cagcagctgt ccaggcacct
     7801 gcagaggctg ctcagagctg ggagaggcct gtgccactgc tgtcagctac agagcctgat
     7861 cttgagcatc ctgtcgcaga tagaaagtca atcagaaaaa tctggttgtg ctcttggatt
     7921 agctgggcat ttggggtaag ggagggactg cagattctga tttgtacaga aaactaaaat
     7981 ttcagtatgt tgcaataaaa tgaaaatatg tcttactcaa
//
