LOCUS       NM_014871               4523 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens poly(A) specific ribonuclease subunit PAN2 (PAN2),
            transcript variant 3, mRNA.
ACCESSION   NM_014871
VERSION     NM_014871.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Reuter,M.S., Zech,M., Hempel,M., Altmuller,J., Heung,T.,
            Polsler,L., Santer,R., Thiele,H., Trost,B., Kubisch,C.,
            Scherer,S.W., Rudnik-Schoneborn,S., Bassett,A.S. and Lessel,D.
  TITLE     Biallelic PAN2 variants in individuals with a syndromic
            neurodevelopmental disorder and multiple congenital anomalies
  JOURNAL   Eur J Hum Genet 30 (5), 611-618 (2022)
   PUBMED   35304602
  REMARK    GeneRIF: Biallelic PAN2 variants in individuals with a syndromic
            neurodevelopmental disorder and multiple congenital anomalies.
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Zhu,M., Zhang,H., Lu,F., Wang,Z., Wu,Y., Chen,H., Fan,X., Yin,Z.
            and Liang,F.
  TITLE     USP52 inhibits cell proliferation by stabilizing PTEN protein in
            non-small cell lung cancer
  JOURNAL   Biosci Rep 41 (10) (2021)
   PUBMED   34533198
  REMARK    GeneRIF: USP52 inhibits cell proliferation by stabilizing PTEN
            protein in non-small cell lung cancer.
REFERENCE   3  (bases 1 to 4523)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4523)
  AUTHORS   Gao,M., Guo,G., Huang,J., Kloeber,J.A., Zhao,F., Deng,M., Tu,X.,
            Kim,W., Zhou,Q., Zhang,C., Yin,P., Luo,K. and Lou,Z.
  TITLE     USP52 regulates DNA end resection and chemosensitivity through
            removing inhibitory ubiquitination from CtIP
  JOURNAL   Nat Commun 11 (1), 5362 (2020)
   PUBMED   33097710
  REMARK    GeneRIF: USP52 regulates DNA end resection and chemosensitivity
            through removing inhibitory ubiquitination from CtIP.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4523)
  AUTHORS   Yang,S., Liu,L., Cao,C., Song,N., Wang,Y., Ma,S., Zhang,Q., Yu,N.,
            Ding,X., Yang,F., Tian,S., Zhang,K., Sun,T., Yang,J., Yao,Z., Wu,S.
            and Shi,L.
  TITLE     USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A
            stabilization
  JOURNAL   Nat Commun 9 (1), 1285 (2018)
   PUBMED   29599486
  REMARK    GeneRIF: USP52 is a bona fide ubiquitin-specific protease, and
            USP52 promotes ASF1A deubiquitination and stabilization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4523)
  AUTHORS   Funakoshi,Y., Doi,Y., Hosoda,N., Uchida,N., Osawa,M., Shimada,I.,
            Tsujimoto,M., Suzuki,T., Katada,T. and Hoshino,S.
  TITLE     Mechanism of mRNA deadenylation: evidence for a molecular interplay
            between translation termination factor eRF3 and mRNA deadenylases
  JOURNAL   Genes Dev 21 (23), 3135-3148 (2007)
   PUBMED   18056425
REFERENCE   7  (bases 1 to 4523)
  AUTHORS   Ezzeddine,N., Chang,T.C., Zhu,W., Yamashita,A., Chen,C.Y.,
            Zhong,Z., Yamashita,Y., Zheng,D. and Shyu,A.B.
  TITLE     Human TOB, an antiproliferative transcription factor, is a
            poly(A)-binding protein-dependent positive regulator of cytoplasmic
            mRNA deadenylation
  JOURNAL   Mol Cell Biol 27 (22), 7791-7801 (2007)
   PUBMED   17785442
REFERENCE   8  (bases 1 to 4523)
  AUTHORS   Yamashita,A., Chang,T.C., Yamashita,Y., Zhu,W., Zhong,Z., Chen,C.Y.
            and Shyu,A.B.
  TITLE     Concerted action of poly(A) nucleases and decapping enzyme in
            mammalian mRNA turnover
  JOURNAL   Nat Struct Mol Biol 12 (12), 1054-1063 (2005)
   PUBMED   16284618
REFERENCE   9  (bases 1 to 4523)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   10 (bases 1 to 4523)
  AUTHORS   Uchida,N., Hoshino,S. and Katada,T.
  TITLE     Identification of a human cytoplasmic poly(A) nuclease complex
            stimulated by poly(A)-binding protein
  JOURNAL   J Biol Chem 279 (2), 1383-1391 (2004)
   PUBMED   14583602
  REMARK    GeneRIF: hPan2 and hPan3 have roles in mRNA decay and exhibit
            cytoplasmic co-localization
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025574.19 and AC073896.41.
            
            On May 1, 2021 this sequence version replaced NM_014871.5.
            
            Summary: This gene encodes a deadenylase that functions as the
            catalytic subunit of the polyadenylate binding protein dependent
            poly(A) nuclease complex. The encoded protein is a magnesium
            dependent 3' to 5' exoribonuclease that is involved in the
            degradation of cytoplasmic mRNAs. Alternate splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the coding region, compared to variant 1. The
            encoded isoform (3) is shorter than isoform 1. Variants 3 and 5
            both encode the same isoform (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024043.1, SRR14038194.2603517.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000440411.8/ ENSP00000388231.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               AC025574.19        57935-58073
            140-535             AC073896.41        574-969
            536-705             AC073896.41        5141-5310
            706-826             AC073896.41        5424-5544
            827-904             AC073896.41        5710-5787
            905-1172            AC073896.41        6151-6418
            1173-1515           AC073896.41        6823-7165
            1516-1612           AC073896.41        7373-7469
            1613-1732           AC073896.41        8328-8447
            1733-1852           AC073896.41        8654-8773
            1853-1981           AC073896.41        9073-9201
            1982-2181           AC073896.41        9289-9488
            2182-2318           AC073896.41        9597-9733
            2319-2425           AC073896.41        9869-9975
            2426-2524           AC073896.41        10184-10282
            2525-2598           AC073896.41        10398-10471
            2599-2746           AC073896.41        10573-10720
            2747-2890           AC073896.41        11024-11167
            2891-2950           AC073896.41        11300-11359
            2951-3041           AC073896.41        11614-11704
            3042-3199           AC073896.41        13761-13918
            3200-3343           AC073896.41        14018-14161
            3344-3523           AC073896.41        14295-14474
            3524-3617           AC073896.41        14601-14694
            3618-3815           AC073896.41        15348-15545
            3816-4523           AC073896.41        16139-16846
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..4523
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /note="poly(A) specific ribonuclease subunit PAN2"
                     /db_xref="GeneID:9924"
                     /db_xref="HGNC:HGNC:20074"
                     /db_xref="MIM:617447"
     exon            1..139
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            140..535
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..3850
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /EC_number="3.1.13.4"
                     /note="isoform 3 is encoded by transcript variant 3;
                     PABP-dependent poly(A) nuclease 2; ubiquitin specific
                     peptidase 52; ubiquitin specific protease 52;
                     PAB-dependent poly(A)-specific ribonuclease subunit 2;
                     inactive ubiquitin carboxyl-terminal hydrolase 52; PAN2
                     poly(A) specific ribonuclease subunit homolog;
                     PAB1P-dependent poly(A)-nuclease; PAN deadenylation
                     complex catalytic subunit 2; PAN deadenylation complex
                     subunit 2; PAB-dependent poly(A)-specific ribonuclease
                     subunit PAN2; PAB1P-dependent poly(A)-specific
                     ribonuclease; poly(A)-nuclease deadenylation complex
                     subunit 2; PAN2-PAN3 deadenylation complex catalytic
                     subunit PAN2"
                     /codon_start=1
                     /product="PAN2-PAN3 deadenylation complex catalytic
                     subunit PAN2 isoform 3"
                     /protein_id="NP_055686.4"
                     /db_xref="CCDS:CCDS8915.1"
                     /db_xref="GeneID:9924"
                     /db_xref="HGNC:HGNC:20074"
                     /db_xref="MIM:617447"
                     /translation="MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPSLLQNVELDPEGV
                     ALEALPVQESVHIMEGVYSELHSVVAEVGVPVSVSHFDLHEEMLWVGSHGGHATSFFG
                     PALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYMARGGLIIFDYLLDENEDMHS
                     LLLTDSSTLLVGGLQNHIIEIDLNTVQETQKYAVETPGVTIMRQTNRFFFCGHTSGKV
                     SLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMR
                     AITPLQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLM
                     TFDVSASKQALAFGDSEGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDL
                     LPLSLIPVPLTTDTLLSDWPAANSAPAPRRAPPVDAEILRTMKKVGFIGYAPNPRTRL
                     RNQIPYRLKESDSEFDSFSQVTESPVGREEEPHLHMVSKKYRKVTIKYSKLGLEDFDF
                     KHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACELGFLFHM
                     LDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQL
                     HQDMQELEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLF
                     TLSYPDDKTGKNYDFAQVLKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVIN
                     CEVNSSKEADFWRMQAEVAFKMAVKKHGGEISKNKEFALADWKELGSPEGVLVCPSIE
                     ELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEEHGVYVYDLMATVVHIL
                     DSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAI
                     LYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVG
                     LDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQ
                     VVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVIN
                     LMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKY
                     LELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVLAL"
     exon            536..705
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            706..826
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            827..904
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1172
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1515
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1612
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1732
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1852
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1981
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2181
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2318
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2425
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2524
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2598
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2746
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2890
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..2950
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3041
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3199
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3200..3343
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3523
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3524..3617
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3618..3815
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     exon            3816..4523
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4490..4495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /note="hexamer: AATAAA"
     polyA_site      4523
                     /gene="PAN2"
                     /gene_synonym="USP52"
                     /note="major polyA site"
ORIGIN      
        1 gccgtagtgg ttaggttgag ccctaggcgt gggggagaac tggggaaact ggaatttccc
       61 gcggagctga cagcgcttgc gctcccccta ctcgttctaa ttccacgcgc tccaaaatat
      121 ccgccatgga gaaatcttgg ccaggatgtc cattctaggc ccatcggtgc tgtcttgctg
      181 aaggttgggt caggcatcta aagggactgt ggtaagggag ggtgtgacac aggtgtaagc
      241 tgccatcgtc atcatgaact ttgagggtct ggaccctgga ctggcagaat atgccccagc
      301 catgcattct gccctggacc ctgtcttgga tgcccacctg aacccaagtc tgctacagaa
      361 tgtggagctg gacccagagg gagtggcctt ggaggctctt cccgtccagg aatcagtgca
      421 cataatggaa ggtgtctact ctgaattgca cagcgtggtg gctgaagtgg gtgtacctgt
      481 ttccgtctcc cactttgact tgcacgagga gatgctgtgg gtggggagcc acgggggcca
      541 tgccacttca ttttttggcc cagccttgga gcgctactca tcctttcaag tcaatggcag
      601 tgatgatatt cggcagatcc agagcctgga gaatggtatc ctttttctca ccaagaacaa
      661 cctcaagtat atggcccgtg ggggcctcat tatatttgat tacctgctgg atgagaatga
      721 ggatatgcac agtctcctac tgactgacag cagcactcta ctcgttggtg ggctgcagaa
      781 tcacataata gagattgatc ttaacactgt ccaggagact cagaagtatg cagtagagac
      841 gcctggagtc accatcatga gacagacaaa tcgcttcttc ttctgcggcc acacgtctgg
      901 caaggtttcc ctgagagacc tccgtacttt taaggtggaa catgagtttg atgccttctc
      961 aggaagtctg tcagactttg atgtgcatgg caacctgcta gctgcctgtg gcttctccag
     1021 ccgcctcact ggcctggcct gcgaccgttt cctcaaggtg tatgatttgc gcatgatgcg
     1081 tgccatcaca ccacttcaag tacatgtgga tcctgccttc ttgcgcttca ttcctacata
     1141 tacttctcgt cttgctatca tctctcagtc agggcagtgc caattctgtg aacccacagg
     1201 cctggccaac ccagccgata tctttcatgt gaatcctgtg gggcctctgc taatgacatt
     1261 tgatgtgtca gccagcaagc aggctctggc ctttggggat tctgagggct gtgtgcacct
     1321 ctggactgat tccccggagc cttccttcaa cccctactcc cgtgagactg agtttgcttt
     1381 gccgtgtctc gtggactcac tgcctcctct ggactggagc caggacctgc tgcctctttc
     1441 cctcatccct gtcccactca ccactgacac acttctctct gattggcctg ctgccaactc
     1501 tgctccagct cccaggcgag caccacccgt ggatgcagag attctgcgca ccatgaagaa
     1561 ggtgggcttc attggctatg cgcccaatcc ccgcaccagg ctgcgcaatc agatacccta
     1621 cagactcaag gagtcagaca gtgaatttga cagcttcagc caggtcactg agtcaccagt
     1681 aggacgagaa gaggaaccac atctccacat ggtttctaag aaataccgca aggtgaccat
     1741 caaatattcc aagctagggc tggaggactt tgacttcaaa cactacaata agaccttgtt
     1801 tgctggatta gagccccaca ttcccaacgc ctactgtaac tgcatgatcc aggtgctcta
     1861 tttcctggag cctgtacgct gtctaattca aaaccacctt tgccagaagg agttctgtct
     1921 ggcatgtgag ctgggcttcc tgtttcacat gttggacctc tctcgtggtg acccttgcca
     1981 gggcaataat tttcttcggg cattccgtac tattcctgag gcctcagccc tcggtctaat
     2041 cctggctgac tcagatgagg cctcaggcaa gggcaatctg gccaggctca ttcagaggtg
     2101 gaatcgcttc attctcactc aactgcatca agatatgcag gagctggaaa taccacaggc
     2161 ttatcgaggt gctggaggca gcagcttttg ctcatcgggg gactctgtta ttgggcagct
     2221 cttcagctgt gagatggaga actgcagcct ctgccgctgt ggcagtgaga ccgtgcgagc
     2281 ctcatccact ctgcttttca cactctccta ccctgatgat aaaactggga agaactatga
     2341 ctttgctcag gtgctgaagc gaagcatctg cctggaccag aatacacagg cctggtgtga
     2401 cacctgtgaa aagtaccagc ccacgattca gacccgcaac atccgccatc tgccagatat
     2461 tcttgtcatc aattgtgagg tgaacagctc aaaagaggct gatttctgga gaatgcaggc
     2521 tgaggttgcc ttcaagatgg cagtaaagaa acacggtggg gaaatctcca agaacaagga
     2581 atttgctttg gctgattgga aggaactagg gagtccagag ggtgtgctgg tgtgtccctc
     2641 cattgaggag ttgaagaacg tctggcttcc tttctccatt cgcatgaaga tgaccaaaaa
     2701 caaagggctg gatgtttgca attggactga tggggatgag atgcagtggg gcccagccag
     2761 ggcagaggag gagcatggtg tctatgtgta tgacctgatg gctactgtgg tacacatcct
     2821 ggactcacgc acagggggca gcctggtggc tcacatcaaa gttggagaga cctaccacca
     2881 gcgcaaggag ggcgttactc accagcagtg gtatctgttc aatgactttc ttattgaacc
     2941 tattgataag catgaagctg tgcagtttga catgaattgg aaagtacctg caatccttta
     3001 ttatgtcaaa cggaatctca attccagata caacctgaac atcaagaacc ctattgaggc
     3061 aagtgtcttg ctggctgaag cctcgctggc acggaagcag cggaaaacac atactacctt
     3121 tattccactg atgctgaatg agatgccaca gattggggac ctggtgggtc tggatgctga
     3181 gtttgtcacc cttaatgagg aggaagcaga gttacgcagt gatggtacca agtctaccat
     3241 taaaccaagc cagatgtcag tagccaggat tacctgtgtt cggggccagg gacccaatga
     3301 gggtatcccc ttcattgatg actacatctc tacccaggag caggtggtgg attacttgac
     3361 tcaatactcg ggtataaagc ctggtgacct cgatgccaaa atttcctcca agcacctaac
     3421 aactctcaag tctacctact taaagcttcg ttttctcatt gacattggag tcaagtttgt
     3481 gggtcatggc ctgcagaagg acttccgggt catcaacctg atggtgccca aggaccaagt
     3541 ccttgacact gtctacctgt tccatatgcc ccgaaaacga atgatttccc tgcgattcct
     3601 tgcttggtac tttctggacc tgaagattca aggggaaacc catgacagta ttgaggatgc
     3661 ccgcacagcc cttcagctgt accgaaagta tctggagcta agcaaaaatg gcactgagcc
     3721 tgagtctttc cacaaggtgc tcaagggtct ttatgagaag ggcagaaaga tggactggaa
     3781 ggtgcctgag cctgagggcc aaacaagtcc caagaatgca gctgtcttct cctcagtgct
     3841 ggcgctctga ctacccttcc caaagaacca cggccctctc cctttactgt tctatagccc
     3901 cagaactggg agatggcttc ctaagttggc tataccttgt ccacttccag tactggacgt
     3961 gctcagggtc tagggtcaca gatggtgcta ttaattgaac tggaacacag cagaattgtt
     4021 gcaaaggttc taggagccag attcattcct tcttcattct ttgcaaaaca gtggtacaga
     4081 catggagtct agaattgacc cagatggaaa gtaattggta ttcttaatat cctgggtgac
     4141 taatatccag gcagagaagc tcctggaacc ataactgtaa gttcctagct ggctagggat
     4201 tgaagtcctg gacagtgaca gaggatacca cagtagttca agacttagca caagtcacca
     4261 actgcttcag ggatacctgg aggggccagc aagtagagtg ttggtggccc aagcaaacca
     4321 gtgttgccaa taccattgcc aaaagggcct ttggatcctg gacaaagctt ggctgccggc
     4381 ttcatttatt cctgctgatg gctgagaagc atctgtcttc catcccactt gcctgtccca
     4441 agttttgttc cattttttaa aaatttgttg taaactgcat gttttataaa ataaaaataa
     4501 aatatcgttt gttatttatc tca
//
