LOCUS       NM_022474               5468 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein associated with LIN7 1, MAGUK p55 family
            member (PALS1), transcript variant 1, mRNA.
ACCESSION   NM_022474
VERSION     NM_022474.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Harms,J., Luttgenau,S.M., Emming,C., Guske,J., Weber,K., Wagner,T.,
            Schowe,L., Nedvetsky,P. and Krahn,M.P.
  TITLE     Pals1 functions in redundancy with SMAP1 to inhibit Arf6 in order
            to prevent Rac1-dependent colorectal cancer cell migration and
            invasion
  JOURNAL   Cancer Gene Ther 30 (3), 497-506 (2023)
   PUBMED   36494580
  REMARK    GeneRIF: Pals1 functions in redundancy with SMAP1 to inhibit Arf6
            in order to prevent Rac1-dependent colorectal cancer cell migration
            and invasion.
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Javorsky,A., Humbert,P.O. and Kvansakul,M.
  TITLE     Structural basis of coronavirus E protein interactions with human
            PALS1 PDZ domain
  JOURNAL   Commun Biol 4 (1), 724 (2021)
   PUBMED   34117354
  REMARK    GeneRIF: Structural basis of coronavirus E protein interactions
            with human PALS1 PDZ domain.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5468)
  AUTHORS   Chai,J., Cai,Y., Pang,C., Wang,L., McSweeney,S., Shanklin,J. and
            Liu,Q.
  TITLE     Structural basis for SARS-CoV-2 envelope protein recognition of
            human cell junction protein PALS1
  JOURNAL   Nat Commun 12 (1), 3433 (2021)
   PUBMED   34103506
  REMARK    GeneRIF: Structural basis for SARS-CoV-2 envelope protein
            recognition of human cell junction protein PALS1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5468)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5468)
  AUTHORS   Luttgenau,S.M., Emming,C., Wagner,T., Harms,J., Guske,J., Weber,K.,
            Neugebauer,U., Schroter,R., Panichkina,O., Petho,Z., Weber,F.,
            Schwab,A., Wege,A.K., Nedvetsky,P. and Krahn,M.P.
  TITLE     Pals1 prevents Rac1-dependent colorectal cancer cell metastasis by
            inhibiting Arf6
  JOURNAL   Mol Cancer 20 (1), 74 (2021)
   PUBMED   33941200
  REMARK    GeneRIF: Pals1 prevents Rac1-dependent colorectal cancer cell
            metastasis by inhibiting Arf6.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5468)
  AUTHORS   Makarova,O., Roh,M.H., Liu,C.J., Laurinec,S. and Margolis,B.
  TITLE     Mammalian Crumbs3 is a small transmembrane protein linked to
            protein associated with Lin-7 (Pals1)
  JOURNAL   Gene 302 (1-2), 21-29 (2003)
   PUBMED   12527193
REFERENCE   7  (bases 1 to 5468)
  AUTHORS   Roh,M.H., Makarova,O., Liu,C.J., Shin,K., Lee,S., Laurinec,S.,
            Goyal,M., Wiggins,R. and Margolis,B.
  TITLE     The Maguk protein, Pals1, functions as an adapter, linking
            mammalian homologues of Crumbs and Discs Lost
  JOURNAL   J Cell Biol 157 (1), 161-172 (2002)
   PUBMED   11927608
  REMARK    GeneRIF: Pals1 functions as an adapter, linking mammalian
            homologues of Crumbs and Discs Lost.
REFERENCE   8  (bases 1 to 5468)
  AUTHORS   Tseng,T.C., Marfatia,S.M., Bryant,P.J., Pack,S., Zhuang,Z.,
            O'Brien,J.E., Lin,L., Hanada,T. and Chishti,A.H.
  TITLE     VAM-1: a new member of the MAGUK family binds to human Veli-1
            through a conserved domain
  JOURNAL   Biochim Biophys Acta 1518 (3), 249-259 (2001)
   PUBMED   11311936
REFERENCE   9  (bases 1 to 5468)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 5468)
  AUTHORS   Kamberov,E., Makarova,O., Roh,M., Liu,A., Karnak,D., Straight,S.
            and Margolis,B.
  TITLE     Molecular cloning and characterization of Pals, proteins associated
            with mLin-7
  JOURNAL   J Biol Chem 275 (15), 11425-11431 (2000)
   PUBMED   10753959
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI245706.1, BC053366.1, AL832936.1, AL832578.2 and AL139785.5.
            
            On May 17, 2019 this sequence version replaced NM_022474.3.
            
            Summary: This gene encodes a member of the p55-like subfamily of
            the membrane-associated guanylate kinase (MAGUK) gene superfamily.
            The encoded protein participates in the polarization of
            differentiating cells, has been shown to regulate myelinating
            Schwann cells (PMID: 20237282), and is one of the components of the
            Crumbs complex in the retina. Mice which express lower levels of
            the orthologous protein have retinal degeneration and impaired
            vision (PMID: 22114289). Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Feb 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.214390.1,
                                           SRR1660803.241404.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261681.9/ ENSP00000261681.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AI245706.1         141-196
            57-1632             BC053366.1         132-1707
            1633-5104           AL832936.1         494-3965
            5105-5226           AL832578.2         4883-5004
            5227-5468           AL139785.5         24446-24687
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.3"
     gene            1..5468
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="protein associated with LIN7 1, MAGUK p55 family
                     member"
                     /db_xref="GeneID:64398"
                     /db_xref="HGNC:HGNC:18669"
                     /db_xref="MIM:606958"
     exon            1..99
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            100..182
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            183..702
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     CDS             336..2363
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="isoform 1 is encoded by transcript variant 1; MAGUK
                     p55 subfamily member 5; stardust; protein associated with
                     lin seven 1; membrane protein, palmitoylated 5 (MAGUK p55
                     subfamily member 5); protein associated with Lin-7 1;
                     membrane palmitoylated protein 5; protein PALS1; protein
                     associated with LIN7 1, MAGUK family member"
                     /codon_start=1
                     /product="protein PALS1 isoform 1"
                     /protein_id="NP_071919.2"
                     /db_xref="CCDS:CCDS9779.1"
                     /db_xref="GeneID:64398"
                     /db_xref="HGNC:HGNC:18669"
                     /db_xref="MIM:606958"
                     /translation="MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLG
                     TRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNP
                     MFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQ
                     NAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTALLNTPHIQ
                     ALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMD
                     SVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIP
                     SQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQA
                     YREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLY
                     NANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFA
                     SAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQV
                     INSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREI
                     IEKTREMEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR"
     exon            703..911
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            912..989
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1136
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1298
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1376
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1560
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1632
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1704
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1872
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2075
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2186
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..5468
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3347..3352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="hexamer: AATAAA"
     polyA_site      3371
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="major polyA site"
     regulatory      5196..5201
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="hexamer: AGTAAA"
     regulatory      5202..5207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="hexamer: ATTATA"
     polyA_site      5214
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="major polyA site"
     polyA_site      5226
                     /gene="PALS1"
                     /gene_synonym="MPP5"
                     /note="major polyA site"
ORIGIN      
        1 attctttatc cggaatttca agggccgccg gagggctgtc gcttctgcag tgcgtaggag
       61 cggccggggc gggaggctcc gcggagccga ggcgtggaga ataagaaagc cttgagtttt
      121 gtgaaaaacc ggagaagaga aactaaaagg acagtagaaa aggcttttcc agtttgcata
      181 atatttcctt catggatact ttttcatagc attattatgt gatgtgagaa gttttttttt
      241 ttgaagtaac atggatttta tactacagaa tcaagagaat tggcttatag gaaaaattga
      301 tttataaaaa gtggtacagg ttttcataga taaccatgac aacatcccat atgaatgggc
      361 atgttacaga ggaatcagac agcgaagtaa aaaatgttga tcttgcatca ccagaggaac
      421 atcagaagca ccgagagatg gctgttgact gccctggaga tttgggcacc aggatgatgc
      481 caatacgtcg aagtgcacag ttggagcgta ttcggcaaca acaggaggac atgaggcgta
      541 ggagagagga agaagggaaa aagcaagaac ttgaccttaa ttcttccatg agacttaaga
      601 aactagccca aattcctcca aagaccggaa tagataaccc tatgtttgat acagaggaag
      661 gaattgtctt agaaagtcct cattatgctg tgaaaatatt agaaatagaa gacttgtttt
      721 cttcacttaa acatatccaa catactttgg tagattctca gagccaggag gatatttcac
      781 tgcttttaca acttgttcaa aataaggatt tccagaatgc atttaagata cacaatgcca
      841 tcacagtaca catgaacaag gccagtcctc catttcctct tatctccaac gcacaagatc
      901 ttgctcaaga ggtacaaact gttttgaagc cagttcatca taaggaagga caagaactaa
      961 ctgctttgct gaatactcca catattcagg cacttttact ggcccacgat aaggttgctg
     1021 agcaggaaat gcagctagag cccattacag atgagagagt ttatgaaagt attggccagt
     1081 atggaggaga aactgtaaaa atagttcgta tagaaaaggc tcgtgatatt ccgttgggtg
     1141 ctacagttcg taatgaaatg gactctgtca tcattagccg gatagtaaaa gggggtgctg
     1201 cagagaaaag tggtctgttg catgaaggag atgaagttct agagattaat ggcattgaaa
     1261 ttcgggggaa agatgtcaat gaggtttttg acttgttgtc tgatatgcat ggtactttga
     1321 cttttgtcct gattcccagt caacagatca agccgcctcc tgccaaggaa acagtaatcc
     1381 atgtaaaagc tcattttgac tatgacccct cagatgaccc ttatgttcca tgtcgagagt
     1441 taggtctgtc ttttcaaaaa ggtgatatac ttcatgtgat cagtcaagaa gatccaaact
     1501 ggtggcaggc ctacagggaa ggggacgaag ataatcaacc tctagccggg cttgttccag
     1561 ggaaaagctt tcagcagcaa agggaagcca tgaaacaaac catagaagaa gataaggagc
     1621 cagaaaaatc aggaaaactg tggtgtgcaa agaagaataa aaagaagagg aaaaaggttt
     1681 tatataatgc caataaaaat gatgattatg acaacgagga gatcttaacc tatgaggaaa
     1741 tgtcacttta tcatcagcca gcaaatagga agagacctat catcttgatt ggtccacaga
     1801 actgtggcca gaatgaattg cgtcagaggc tcatgaacaa agaaaaggac cgctttgcat
     1861 ctgcagttcc tcatacaacc cggagtaggc gagaccaaga agtagccggt agagattacc
     1921 actttgtttc gcggcaagca ttcgaggcag acatagcagc tggaaagttc attgagcatg
     1981 gtgaatttga gaagaatttg tatggaacta gcatagattc tgtacggcaa gtgatcaact
     2041 ctggcaaaat atgtctttta agtcttcgta cacagtcatt gaagactctc cggaattcag
     2101 atttgaaacc atatattatc ttcattgcac ccccttcaca agaaagactt cgggcattat
     2161 tggccaaaga aggcaagaat ccaaagcctg aagagttgag agaaatcatt gagaagacaa
     2221 gagagatgga gcagaacaat ggccactact ttgatacggc aattgtgaat tccgatcttg
     2281 ataaagccta tcaggaattg cttaggttaa ttaacaaact tgatactgaa cctcagtggg
     2341 taccatccac ttggctgagg tgaaagaaac atccattctg tggcatgttg gacttgatct
     2401 ggcaaaaact gccaatagga ggactgcccg acactgcagc aagattgagg ataagatgga
     2461 aggcagcagt ataagctgta gatctgttct tagatctctt gaattagtga gacgacagtt
     2521 cccttaggca gtttgtgcat ggcatccttt attctctata catggcttta gcggttcttg
     2581 cctcattttg ggattctaaa tggaagcttt caacagagca ttccattttg tcctgttaaa
     2641 accttttgtt ttcacctaaa ccctttctgc ttagttgtat ctctgtgaaa aacttgtata
     2701 cacaagcgtc catgtctcac acaaatattg atgtgattat tcttaagtgt taaatcatta
     2761 acacttaaat gacttcattg ggaatattga gcagagggac tgtgcttcta tgcactgggc
     2821 aaggcagtat ttgcttagga aactaattta gtcatcagag atactttcct aaaaaggaaa
     2881 aataaaaaac aaaatggtgc cactttgggt tgaagctact ttgttaggct tgaattcatt
     2941 tatatgtctt ttgattctta aaaaaacaaa aaacattcca ttagaagcac cagttttttt
     3001 gctcagactt tgtggatcag actctacact caacacactc taatctactt aaaggtatac
     3061 aaaatatgct gatctttttt aaattatgat ttcctgaatt tttttcttaa gtcgtctcaa
     3121 ctgatttact cacttagctt cccttccctc atcagcatag tataatagaa tgtatgttac
     3181 atttttatga atggcaggtg ttcattataa tctgtattga cttaaaaagt ttcttcctca
     3241 tgatgctaat agttttttgt atacatggga ggatagcaca tttgacagtt tttgcatttt
     3301 tatgtatgag cacagtatcc tatgactgtg ctacgtatat ataggtaata aactggaatt
     3361 ctgttgatga atatagctgc tgtactgtat attaatattt aatagatcaa caaatggtca
     3421 ttgaaaacac ttgtttagca ttagaataaa attatatatg tccttgggaa atattatgac
     3481 agttgacttt aagatcaaaa ggaagggaag acctgaaagt catttgaaca ttttaggaaa
     3541 agaatattgg agagaaaaag gtattaaata tatagaaata ggtttttaac ctaacaaggt
     3601 ctgcctctta tgacgagaat gcaacagctt ggtaaatcat aaaagaaaca tttaagctaa
     3661 taggattttc gtactgtctc tatagctgta gctttaaaat tcaacgtata taattggcat
     3721 ggaaacttaa tttgcagtct tttcaagcct ttaggatagt gtgatgtgta acaaacaacc
     3781 tcaaatgtga atgccttgat tttattttta tggtgacttt agctacagca tttcctatac
     3841 ccagagctaa acactggaat aatactgaca tcatttaatt taacataagc aattatgttt
     3901 aaggagtaat ttgtgtcatg tacatatttg attgattttt tttcttctac ataattttat
     3961 ttgaacaaat gtagacagtt tatatgtcgc ctttttctgt tcaaatttgc atggcctatt
     4021 aagttggctg gagagtgttt tatgtggaaa tattttcaag ataatgttcc ttaggaagaa
     4081 aataacattc ttgggttgag ggaaggaatg ccatacacta ctgtctcttc agatctgaaa
     4141 tactccagtt tagagccagg aaatttcaca ggtcacaccg atttttagca ttaaaaacta
     4201 aggaatatac ttagcactta cttaatcttt tcagttttcc agtttacgtc tcaggaatga
     4261 agtgtagtct atggttgaca atggagtttt gtgatcctgc ttattgtaac tgacaactgt
     4321 tttcaactcc aagagctaaa ctattggcag ttcatgttaa gttagagtga gggtgtaggt
     4381 agtgtcagtg agtggctctt gtgcctgctg tagacattag gcctgcacta gggccatgtg
     4441 ctgtcaagat tcaggaacat ggctttaaca agcagatctt gtatcaaggc agaggtgatg
     4501 ccatgccata cttttaggaa gtctgagatg ataaatattt caaggtcagt gaagtctatc
     4561 aatcattctc cccttcctca tcagcaatgg tagatagaaa tgtcctaaac ttttctaaat
     4621 cctagtgatg aggatgtgct gatattcaac atagtcctta aagtgaaaac tgagttgttg
     4681 ctgacctcca caaaagaata tggaaaaaag ccttgctgta cacctagttg tacagccact
     4741 ctggccaatt ccatttcctg tccctctgtg gttctgactg gagaccccag tgtgggggag
     4801 gtcttaccat ttaatataga aatgatatca ataactaatg ctatgtactt ggaaaatcca
     4861 aataaggaag ttttaggttg gtgcataact ttgtttctca aattttcgtt gtcagaacaa
     4921 atggaaggag aatattattt agactaatcc agatttgctt tctatgaaaa tctaatgtct
     4981 ggattatctt ccttttctca tggcctaaga aataaggatc aataaggaat gatttgaatg
     5041 taattttgtg aatgtgtgga aaatataaag cagggattta gccttaataa aggtaacctt
     5101 ctgacatctg ttgttaatcc ccctttgtac tcttttcctg tatctgcact gttattttga
     5161 gatgtcatac tgtacactgt attgtaaaaa taaaaagtaa aattatattt caaattttaa
     5221 aagccactaa gcaatttctc ttgctatttg ctcggccact caaggtttcc acacatgtaa
     5281 agaccaccct cctcttcaag tttttgccaa aagcaagacc attggatcct ccagctacaa
     5341 cacaaaatac tttttgattt gttctttaca gagggatagt gccccctgtt ggcacaggta
     5401 taggtaggtc ctgtttcctt aattattaaa accaacaact aagtgtatta aagatggaca
     5461 acgcaagg
//
